Publications

[mycoplasmas and pregnancy. preliminary study].	the prevalence of genital mycoplasmas was studied among 191 pregnant women followed up at diaconesses hospital, paris. ureaplasma urealyticum was recovered from 65% of patients, alone (54%) or in association with mycoplasma hominis (11%). the relationship to pregnancy outcome, the effect of erythromycin treatment, the interest of biological markers of infection (mycoplasmal quantitation and serologic tests, c-reactive protein) and the role of other known genital pathogens were investigated. vagi ...	1992	1624724
detection of mycoplasma contamination through modulation (stimulation or inhibition) of thymidine incorporation by unstimulated mouse spleen cells.	in the present report we describe a rapid and sensitive assay for mycoplasma detection in cell cultures. the assay is based on the ability of contaminated culture supernatants to modulate [3h]tdr incorporation by unstimulated mouse splenocytes. several mycoplasma species (mycoplasma orale, culturable and non-culturable strains of mycoplasma hyorhinis) inhibited [3h]tdr incorporation and permitted the detection of some contaminated cell cultures that would otherwise have escaped detection in assa ...	1992	1640109
mycoplasma hominis infection of perihepatic hematomas in a liver transplant recipient.	we report the case of a recipient of liver transplantation in whom postoperative perihepatic hematomas were infected by mycoplasma hominis. etiologic diagnosis was delayed because this organism is a rare cause of postoperative infection and usually does not grow on standard bacteriologic media. the role of m. hominis in postoperative infections and the diagnostic problems of this organism are discussed.	1992	1728082
septic arthritis and bacteremia due to mycoplasma resistant to antimicrobial therapy in a patient with systemic lupus erythematosus.	we report a case of septic arthritis caused by mycoplasma hominis in a patient with systemic lupus erythematosus. the infection started as monarthritis but spread to at least four joints despite apparently suitable therapy with various antimicrobial agents, including doxycycline, clindamycin, and ciprofloxacin; subsequent bacteremia was documented. control was ultimately achieved with use of the experimental fluoroquinolone temafloxacin in combination with doxycycline administration, arthroscopi ...	1992	1520784
[epidemic investigation of genital mycoplasma hominis infection in women].	the genital mycoplasma hominis infection in women was investigated at xiaguan district, nanjin in june, 1990. leucorrheas from 722 women were tested with elisa for the antigen of m. hominis. the prevalences of genital m. hominis were not statistically different among age and occupation groups. women having pregnancy more than three times had a significantly higher infection rate, it is suggested that induced abortion might provide chances of the infection. the prevalence was significantly differ ...	1992	1394388
[significant bacteriospermia. value and limits of sperm count in andrology].	the data concerning bacteriospermia--are very different in the literature. asymptomatic andrologic patients, and as control group--patients of the outpatient department for male adnexitis--were studied. cultivation of aerobic bacteria and mycoplasmas has been recently accomplished by quantitative bacteriological analysis and chlamydia diagnostic. the microbiological screening of 210 andrologic patients ejaculate has given the following results: 19.6% were sterile, 21.0% were colonised by apathog ...	1992	1408103
in vitro activity of several antimicrobial agents against genital mycoplasmas.	the increasing resistance of genital mycoplasmas to tetracyclines is a serious problem, since this group of antibiotics is one of the few that is effective against virtually all species of mycoplasmas. tetracyclines are also used to treat many sexually transmitted diseases. in this study, we assessed the susceptibility of mycoplasma hominis and ureaplasma urealyticum to macrolides, tetracyclines, spectinomycin, and trospectomycin by the agar dilution method. for m hominis, trospectomycin was the ...	1992	1468088
high incidence of asymptomatic urogenital infection in patients with uveitis anterior.	acute anterior uveitis (aau) may be associated with systemic infectious or inflammatory disease. we examined 92 patients with the first attack of acute anterior uveitis; all patients were free of any extraocular symptoms. a thorough clinical examination did not reveal any systemic underlying disease. in the course of microbiological examination, however, a high incidence of asymptomatic infection of the urethra and/or cervix with ureaplasma urealyticum, chlamydia trachomatis and mycoplasma homin ...	1992	1303857
prerequisite work-up of the couple before in-vitro fertilization.	prerequisite examinations, including immune status for rubella and hepatitis b antigens, cultures for chlamydia trachomatis and mycoplasmas, pap smear and hystersalpingogram were performed in 227 couples before their enrollment into an in-vitro fertilization (ivf) programme. the examinations were completed in 187 couples. immune status for rubella had already been documented in 45% of the women: of the remainder, 11 patients were not immune (6%). a screening test for hepatitis b antigens had alr ...	1992	1522189
the prevalence and clinical significance of intraamniotic infection with candida species in women with preterm labor.	intraamniotic infection is considered a major etiologic factor of preterm birth. positive amniotic fluid cultures are rarely contaminated with candida species. the presence of this microorganism is associated with a poor pregnancy outcome. out of 773 transabdominal amniocenteses performed in women presenting with preterm labor and intact membranes, 77 patients (9.9%) had positive amniotic fluid cultures and in 5 women (6.5%) candida species were identified. on the other hand, 625 amniocenteses w ...	1992	1550392
[immunoreactivity of mycoplasma hominis pg21 antigens and human sperm antigens].	the authors selected from the who bank for reproductive immunology ten sera containing concurrently antispermatozoal antibodies and antibodies against mycoplasma hominis pg21. by absorption examination of selective sera with a different concentration (10(9)/ml, 3.10(8)/ml, 10(8)/ml) of human spermatozoa and a rinsed sonicate of mycoplasma hominis pg21 in dilutions of 1:20, 1:80, 1:320 the authors did not find a crossed immunoreactivity between sperm antigens and mycoplasmic antigens.	1992	1473166
ureaplasma urealyticum and mycoplasma hominis in women with systemic lupus erythematosus.	to determine the prevalence of genitourinary mycoplasma infection in women with systemic lupus erythematosus (sle).	1992	1567492
adverse outcome in pregnancy following amniotic fluid isolation of ureaplasma urealyticum.	infections in pregnancy with ureaplasma urealyticum have been associated with a wide range of adverse outcomes, such as early abortion, stillbirth, prematurity, and neonatal morbidity and mortality. causality has been difficult to demonstrate secondary to the high prevalence of asymptomatic lower genital tract (lgt) colonization and culture data from inaccessible or potentially contaminated sites. between 1985 and 1989, 2461 second-trimester genetic amniocenteses were evaluated at the cytogeneti ...	1992	1553356
nucleotide sequence of the arginine deiminase gene of mycoplasma hominis.	the arginine deiminase gene of mycoplasma hominis was amplified by the polymerase chain reaction, and its entire nucleotide sequence was determined. this gene consists of 1227 base pairs encoding 409 amino acids, and has 35.2% guanine plus cytosine content. nucleotide sequence homologies of the arginine deiminase gene between m. hominis and m. arginini, and between m. hominis and m. orale were 82.1 and 80.8%, respectively, suggesting that this gene is highly conserved among arginine-utilizing my ...	1992	1522817
rapid detection of tetm in mycoplasma hominis and ureaplasma urealyticum by pcr: tetm confers resistance to tetracycline but not necessarily to doxycycline.	tetracycline resistance in mycoplasma hominis and ureaplasma urealyticum has been associated with the tetm determinant and has recently been increasing in incidence. we report here a rapid method for detection of the tetm determinant based on the use of the polymerase chain reaction (pcr) to amplify a 397-bp dna fragment from the tetm gene and verification of specificity using the restriction enzyme taqi. analysis of 42 u. urealyticum and 49 m. hominis isolates indicates that the pcr method may ...	1992	1526460
lumbar pain caused by mycoplasma infection.	a 45-year-old woman was admitted to hospital following acute onset of lower back pain. clinical and laboratory investigations established a lumbar paraspinal soft tissue infection with mycoplasma hominis associated with severe spondylarthrosis at l5/s1. a relationship to a recently performed hysterectomy must be considered.	1992	1533852
physical and genetic mapping of the genomes of five mycoplasma hominis strains by pulsed-field gel electrophoresis.	we present the complete maps of five mycoplasma hominis genomes, including a detailed restriction map and the locations of a number of genetic loci. the restriction fragments were resolved by field inversion gel electrophoresis or by the contour-clamped homogeneous-electric-field system of pulsed-field gel electrophoresis. all the apai, smai, bamhi, xhoi, and sali restriction sites (total of 21 to 33 sites in each strain) were placed on the physical map, yielding an average resolution of 26 kb. ...	1992	1551841
[role of mycoplasmas in the last month of pregnancy and postpartum pathology. prospective study of 577 pregnancies].	the authors report the findings of a prospective study of 577 pregnancies. the purpose of this study was to determine the influence of mycoplasma on peri-post-natal illness arising during the last month of gestation and during the immediate post-partum period. the incidence of contamination was 29% of ureaplasma urealyticum and 2.3% for mycoplasma hominis. this showed little change post partum. the rate of transmission to the fetus or placenta is low. no difference in end-of-term fetal weight wa ...	1992	1579798
comparison of commercially available media for detection and isolation of ureaplasma urealyticum and mycoplasma hominis.	the mycotrim triphasic flask system (irvine scientific, irvine, calif.) was compared with a system composed of mycotrim gu broth (irvine scientific) and a7 or a8 agar (remel, lenexa, kans.) for the ability to detect ureaplasma urealyticum and mycoplasma hominis from 129 genital specimens. of the 64 specimens positive for u. urealyticum, 25, 98, and 100% were detected on mycotrim triphasic agar and a7 and a8 agars, respectively. all 18 specimens that grew m. hominis were detected by a7 and a8 aga ...	1992	1583143
ovarian actinomycosis presenting as acute peritonitis.	a patient with acute peritonitis due to ovarian actinomycosis and the association of this disease with the intrauterine contraceptive device is discussed. because adequate treatment requires prolonged antibiotics the diagnosis should be sure. unfortunately if antibiotics are given pre-operatively culture is usually unsuccessful and thus careful examination of all removed material is essential. as many of these women are of child bearing age surgical intervention should aim to preserve fertility ...	1992	1586308
[ureaplasma urealyticum and mycoplasma hominis: incidence and clinical significance of their isolation in the perinatal period].	in order to known the clinical significance of the colonization of the maternal genitalia and infant with genital mycoplasmas, we studied prospectively 219 pairs of mothers and newborns admitted for delivery to a general public hospital. u. urealyticum and m. hominis were isolated from 72 (32.9%) and 10 (4.6%) of the mothers, respectively. during the first 4 days of life. u. urealyticum and m. hominis were isolated from 23 (10.5%) and 1 (0.5%), of the newborn infants respectively. maternal colon ...	1992	1605412
comparison of ofloxacin and metronidazole for the treatment of bacterial vaginosis.	the efficacy and safety of ofloxacin, 200 mg twice daily for 7 days, was compared with metronidazole, 400 mg twice daily for 7 days, for the treatment of bacterial vaginosis (bv). diagnosis of bv was confirmed by at least 3 of the following 4 criteria: the presence of an abnormal vaginal discharge on examination, clue cells on microscopy of vaginal specimens, vaginal ph greater than 5.0 and a positive amine test. vaginal specimens were examined for mobiluncus spp, analysed for the succinate/lact ...	1992	1616967
a randomized, comparative study of the efficacy and tolerance of roxithromycin and doxycycline in the treatment of women with positive endocervical cultures for chlamydia trachomatis and mycoplasma spp. in an in vitro fertilization program.	a total of 45 patients wishing to enter an in vitro fertilization program had positive endocervical cultures for chlamydia trachomatis, 86 had positive cultures for ureaplasma urealyticum, and 11 had positive cultures for mycoplasma hominis. these patients and their consorts were randomly allocated to treatment with either roxithromycin or doxycycline. both antibiotics were completely effective against c. trachomatis. in patients with u. urealyticum infection, roxithromycin was successful in 73% ...	1992	1617924
microbial causative agents of male urethritis.	the incidence of chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis was studied in men with urethritis. out of 150 examined men 48.7% had the positive isolation of u. urealyticum, 26.0% c. trachomatis, 22.7% n. gonorrhoeae, 18.7% m. hominis and in one (0.7%) patient t. vaginalis was found. none of the above mentioned microorganisms was detected in 24.7% of examined men. in 43.3% cases only one agent was isolated. in 23.3% of the men ...	1992	1619287
the prevalence of potential pathogenic micro-organisms in the endocervix of pregnant women at tygerberg hospital.	the prevalence of potential pathogenic organisms cultured from the endocervix in 206 pregnant patients was: ureaplasma urealyticum 63%, mycoplasma hominis 39%, candida albicans 20%, chlamydia trachomatis 11%, neisseria gonorrhoeae 7%, group b beta-haemolytic streptococcus 6% and listeria monocytogenes 1%. the most common complications of pregnancy found were: preterm labour 14%, urinary tract infection 8% and premature rupture of membranes 4%. significantly more patients in whom c. trachomatis o ...	1992	1734556
the relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women.	lactobacilli provide an important microbial defense against genital colonization by pathogens. the role of hydrogen peroxide (h2o2) in the control of genital microflora was explored in a cross-sectional study of 275 women in the second trimester of pregnancy. vaginal cultures were obtained for detection of h2o2-positive and h2o2-negative lactobacilli and other members of the genital microflora. compared with women with h2o2-negative lactobacilli, women colonized by h2o2-positive lactobacilli wer ...	1992	1738516
a longitudinal study of pelvic inflammatory disease.	to study the microbiology and long term prognosis of pelvic inflammatory disease (pid).	1992	1477024
gardnerella vaginalis: characteristics, clinical considerations, and controversies.	the clinical significance, gram stain reaction, and genus affiliation of gardnerella vaginalis have been controversial since gardner and dukes described the organism as the cause of "nonspecific vaginitis," a common disease of women which is now called bacterial vaginosis. the organism was named g. vaginalis when taxonomic studies showed that it was unrelated to bacteria in various genera including haemophilus and corynebacterium. electron microscopy and chemical analyses have elucidated the org ...	1992	1498765
mycoplasma hominis in women with bacterial vaginosis.	an association between m. hominis and bacterial vaginosis (bv) was looked for in total 205 women characterized as having bv. vaginal specimens from these patients were studied for the presence of amines, polymorphonuclear leukocytes, clue cells, and lactobacilli, and were also cultured to isolate gardnerella vaginalis, m. hominis, trichomonas vaginalis, candida albicans and anaerobes. g. vaginalis was found to be the most common isolate from 132 cases (64.3%). m. hominis was isolated from 90 cas ...	1992	1506065
fatal intrauterine infection associated with mycoplasma hominis.		1992	1420708
quinolones as an alternative treatment of chlamydial, mycoplasma and gonococcal urogenital infections.	we report the results of several trials aimed at evaluating the quinolones in urogenital infections. in chlamydia trachomatis infections, ofloxacin (200 mg b.i.d. for 10 days) gave a cure rate of 98% (n = 66), and fleroxacin (400 mg s.i.d. for 7 days) provided a cure rate of 89% (n = 19). a double-blind study comparing fleroxacin (600 mg s.i.d.) to doxycycline (100 mg b.i.d.) for 7 days showed similar high cure rates for both regimens (100%; n = 23). in mycoplasma hominis infections, ofloxacin ( ...	1992	1421625
[study on the infections of u. urealyticum, m. hominis, and c. trachomatis in patients with venereal diseases and healthy controls in three areas of china].	two hundred and thiry-nine patients with venereal diseases and 264 healthy controls, from yichang, liuzhou, and beijing, were detected for the antibodies to ureaplasma urealyticum (uu). mycoplasma hominis (mh), and chlamydia trachomatis (ct) by indirect hemagglutination (iha) in 1990. the results showed that: 1) in patients with venereal diseases, the positive rates was the highest in ct (27.62%), higher in uu (17.57%), and lower in mh (4.18%); 2) the levels of antibody to ct and uu were signifi ...	1992	1301266
[epidemiological study on mycoplasmas colonization and infection in the female genital tract].	1309 cervical or vaginal specimens from females in various physiological periods and women with multiple sexual partners were examined for ureaplasma urealyticum and mycoplasma hominis from feb. to may, 1990. significantly, low carriage rates of u.urealyticum were found in the neonatal (28.57%), puerperium (26.51%) and postmenopause (30.00%) groups, whereas, pregnant women (more than 36 weeks) showed the highest incidence (73.20%). the recovery rate of u. urealyticum in girls with no history of ...	1992	1303321
[uses of vaginal ph measurement for prevention of premature labor. results of a prospective study].	the influence of the microflora of the vagina and cervix has been undervalued for the induction of premature birth. examination of the microbiological problem by determination of the vaginal ph in combination with the microflora (chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum). report of the outcome of 161 prospective, ad the beginning normal pregnancies in comparison of 200 primary hospitalized pregnancies with symptoms of imminent premature birth. conclusion: the vaginal ph ...	1992	1290278
in vitro activity of sparfloxacin and other antimicrobial agents against genital pathogens.	the in vitro activity of sparfloxacin was determined for 60 strains of neisseria gonorrhoeae, 15 strains of chlamydia trachomatis and 40 strains each of gardnerella vaginalis, mycoplasma hominis, and ureaplasma urealyticum and compared with those of ampicillin, azithromycin, clarithromycin, erythromycin, ofloxacin, temafloxacin and tetracycline. sparfloxacin was active against all the strains studied and appeared to be the most potent quinolone tested. sparfloxacin had the lowest mics against n. ...	1992	1337506
vaginal microbial flora as a cofactor in the pathogenesis of uterine cervical intraepithelial neoplasia.	the vaginal microbial flora of 106 women with histopathologically confirmed cervical intraepithelial neoplasia and 79 women without disease, was evaluated for gardnerella vaginalis, trichomonas vaginalis, candida albicans and other yeasts. flora morphology was assessed by gram staining of secretions. cervical cultures were examined for herpes simplex virus, cytomegalovirus and neisseria gonorrhoeae. chlamydia trachomatis antigens in cervical secretions were detected by enzyme immunoassay. human ...	1992	1351005
lack of activity of zidovudine against ureaplasma urealyticum and mycoplasma hominis.		1992	1354810
decreased metabolism and viability of mycoplasma hominis induced by monoclonal antibody-mediated agglutination.	monoclonal antibodies (mabs) were generated against lysates of clinical mycoplasma hominis isolates. three of these, designated bg2, ba10, and fe6, recognized an integral membrane protein of m. hominis with an apparent molecular weight of 50,000 (p50). electron microscopy studies demonstrated that this protein is distributed evenly over the cell surface. these anti-p50 mabs were species specific for m. hominis; they reacted with 42% of 126 tested clinical m. hominis isolates and showed no reacti ...	1992	1370272
[epidemiological factors and vaginal flora changes in vaginal bacteriosis (bacterial vaginosis)].	the aim of this study was to know the modifications of the vaginal bacterial flora that occurs in bacterial vaginosis and to know the involvement of these microorganisms and the influence of several epidemiologic factors in the etiology of this disease.	1992	1391016
sexually transmitted diseases (std) in infertile males attending the andrology clinic at ga-rankuwa hospital.	sexually transmitted diseases (stds) affect the physiology of male/female reproduction. chronic bacterial infection of semen is uncommon, but may be a cause of male infertility. antibacterial treatment results in improvement in sperm quality, once the infection is eradicated. little is known about how infection with mycoplasma hominis affects semen quality, but treatment with antibiotics improves motility and decreases the percentage of coiled tails. chlamydia trachomatis is not frequently i ...	1992	12286085
presence of chlamydia, mycoplasma, ureaplasma, and other bacteria in the upper and lower genital tracts of fertile and infertile populations.	the genital mycoplasmas (mycoplasma hominis and ureaplasma urealyticum) and chlamydia trachomatis have been implicated as possible etiologic factors in infertility. their role in patients with infertility needs to be further defined.	1993	18475323
the normal vaginal flora, h2o2-producing lactobacilli, and bacterial vaginosis in pregnant women.	in this study of the vaginal flora of 171 pregnant women in labor at term, the flora was categorized as normal (lactobacillus predominant), intermediate, or representative of bacterial vaginosis (bv) on the basis of a vaginal smear. bv was diagnosed in 39 women (23%); the vaginal flora was classified as normal in 50% of cases and as intermediate in 27%. h2o2-producing lactobacilli were recovered from 5% of women with bv, 37% of those with an intermediate flora, and 61% of those with a normal flo ...	1993	8324131
microbial invasion of the amniotic cavity during term labor. prevalence and clinical significance.	the purpose of this study was to determine the prevalence and clinical significance of microbial invasion of the amniotic cavity during spontaneous parturition at term. amniotic fluid was retrieved by transabdominal amniocentesis from 90 women in spontaneous term labor with intact membranes. fluid was cultured for aerobic and anaerobic bacteria, including mycoplasma. the prevalence of positive amniotic fluid cultures was 18.8% (17/90). the most common microbial isolates were ureaplasma urealytic ...	1993	8410850
sexual intercourse during pregnancy and preterm delivery: effects of vaginal microorganisms. the vaginal infections and prematurity study group.	our aim was to investigate the influence of vaginal colonization with specific microorganisms on the relationship between sexual intercourse during pregnancy and preterm delivery.	1993	8438920
susceptibility of mycoplasmas to antimicrobial agents: clinical implications.	mycoplasmas are susceptible to antimicrobial agents that affect dna, rna, protein synthesis, or the integrity of the cell membrane. mycoplasmas are not susceptible to agents that interfere with synthesis of folic acid or that act on the cell wall. tetracyclines, erythromycin, clindamycin, chloramphenicol, aminoglycosides, and fluoroquinolones have been shown to have activity against one or more mycoplasmal species. tetracycline-resistant isolates of mycoplasma hominis and ureaplasma urealyticum ...	1993	8399916
extragenital mycoplasma hominis infections in adults: emphasis on immunosuppression.	mycoplasma hominis, a commensal organism that is potentially pathogenic both in maternal perinatal and in neonatal infections, also causes nongenitourinary infections in adults. we reviewed the clinical features of cases from the literature and emphasized recent cases. infection sites were classified as blood, vascular sites, wounds, central nervous system, joints, and respiratory tract. twenty-one of 31 newly summarized cases and 32 of 67 overall cases were associated with immunosuppression and ...	1993	8399923
identification of mycoplasmas in urine from persons infected with human immunodeficiency virus.	voided urine samples from persons with and without human immunodeficiency virus (hiv) infection were examined for mycoplasmas. mycoplasma hominis organisms were identified in cultures of urine from 32 (18%) of 180 hiv-positive individuals and from 8 (21%) of 38 hiv-negative individuals. in contrast, glucose-utilizing mycoplasmas were identified in the urine of 30 (17%) of the hiv-positive individuals and in none of those who were hiv-negative. assays of growth inhibition around disks containing ...	1993	8399927
models of infection due to mycoplasmas, including mycoplasma fermentans, in the genital tract and other sites in mice.	progesterone induces susceptibility in murine genital tracts to mycoplasma pulmonis, mycoplasma pneumoniae, and mycoplasma genitalium when each is given intravaginally. in contrast, estradiol, but not progesterone, induced such susceptibility to ureaplasma urealyticum, mycoplasma hominis, and mycoplasma fermentans, the "incognitus" and pg18 strains of the latter behaving similarly. none of the mice that had been given m. fermentans intravaginally died, whereas others that had been given large nu ...	1993	8399930
[the definition and epidemiology of bacterial vaginosis].	bacterial vaginosis is a condition with a flora change in the vaginal where a lactobacilli-dominated flora is exchanged with an abundant complex flora dominated by strict and facultative anaerobic bacteria. the condition seldom occurs in prepubertal girls and post-menopausal women, suggesting an hormonal component in its etiology. recurrent episodes of bacterial vaginosis are frequent. three out of four criteria should be fulfilled for establishing the diagnosis of bacterial vaginosis, i.e., an ...	1993	8484086
[recent bacteriologic data: from physiopathology to treatment].	bacterial vaginosis is characterized by a change in the vaginal ecosystem in which lactobacillus spp, the dominant members of the normal flora, are replaced by an association of various bacterial species including gardnerella vaginalis, anaerobes (bacteroides spp, prevotella spp, porphyromonas spp, peptostreptococcus spp and mobiluncus spp) and mycoplasma hominis. the reasons for this imbalance are unknown, although the loss of lactobacilli that produce hydrogen peroxide (which is toxic for g. v ...	1993	8484089
mycoplasmalike organisms (mlos) and distinguish them from other mlos.	dna of 10 lines of rice yellow dwarf (ryd) mycoplasmalike organisms (mlos) from japan, the phillippines, and thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal dna of a tochigi (japan) line of ryd mlo. one chromosomal probe (ryd9) and all six extrachromosomal probes hybridized with various other mlos (sugarcane white leaf, onion yellows, cineraria witches'-broom, japanese hornwort witches'-broom, water dropwort wiches'-broom, gentian witches'-b ...	1993	8489230
characteristics of mycoplasma hominis adhesion.	mycoplasma hominis, a human pathogen, has previously been observed to bind to sulfatide separated on thin-layer chromatograms. it has not been demonstrated, however, that the binding is not simply a nonspecific ionic interaction. the ability of a low-passage patient isolate of m. hominis to adhere to glycoconjugates other than sulfatide and the characteristics of its binding to sulfatide were studied. mycoplasmas were found to bind strongly and specifically in a temperature- and dose-dependent m ...	1993	8491739
efficacy of intravaginal 0.75% metronidazole gel for the treatment of bacterial vaginosis.	to assess the efficacy of 0.75% metronidazole vaginal gel, formulated at ph 4.0, for the treatment of bacterial vaginosis in a double-blind, placebo-controlled crossover trial.	1993	8497364
prevalence of cervical pathogens in women with and without inflammatory changes on smear testing.	to assess correlation between nonspecific cervicitis, inflammation, or exudate on cervical smears tests and confirmed presence of known cervical pathogens.	1993	8499824
mycoplasma hominis sternal wound infection and bacteraemia.	mycoplasma hominis is a rare cause of bacteraemia in adult males. we believe this report to be the first of mycoplasma hominis bacteraemia and wound infection complicating cardiac surgery. because of difficulties in isolating the organism, cases may be missed. review of the literature on m. hominis bacteraemia in adult males reveals that infection is often mild and most often associated with urethral catheterisation. m. hominis is resistant to many antibiotics including erythromycin. if treatmen ...	1993	8505567
etiology of acute pelvic inflammatory disease proven by laparoscopy.	only a few studies have investigated the etiology of acute pelvic inflammatory disease (pid) in laparoscopy-proven cases using pelvic samples for culture. most of these studies were conducted in north america and scandinavia.	1993	8511710
cytoadhesins of mycoplasma hominis.	the mechanism of mycoplasma hominis adherence to host cells of the urogenital tract was investigated with monoclonal antibodies (mabs) directed against antigenic surface-localized polypeptides p50, p60, p80, and p100 of cytoadherent m. hominis fbg. a cell enzyme-linked immunosorbent assay was established allowing quantification of cytoadherent mycoplasmas detected by one of the following mabs: four mabs directed against p100 (molecular weight, about 100,000), three mabs against p80, one mab agai ...	1993	8514399
failure of tracheal aspirate cultures to define the cause of respiratory deteriorations in neonates.	the spectrum of organisms responsible for lower respiratory tract infection in chronically ventilated neonates is poorly defined. during an 18-month period 63 infants with a respiratory deterioration defined as an increase in fractional inspired o2 concentration > or = 20% and/or mean airway pressure > or = 3 cm h2o were evaluated for pulmonary infection. these infants were compared with 58 stable control ventilated infants. tracheal aspirates for culture and gram stain were taken from both grou ...	1993	8345996
mycoplasma hominis and ureaplasma urealyticum in neonates with suspected infection.	the role of genital mycoplasmas in the pathogenesis of neonatal infection is incompletely understood. we performed nasopharyngeal, blood and cerebrospinal fluid (csf) cultures for mycoplasma hominis and ureaplasma urealyticum in 69 neonates who underwent a diagnostic workup for suspected sepsis. the mean gestational age was 35.9 weeks (range, 25 to 42 weeks) with a mean birth weight of 2386 g (range, 652 to 4420 g). twenty-seven infants (39.1%) had positive nasopharyngeal cultures; 6 were positi ...	1993	8345998
the microbiology of bacterial vaginosis.	a group of microorganisms are present concurrently in high concentrations in the vaginas of women with bacterial vaginosis. the major members of the group are gardnerella vaginalis, anaerobic gram-negative rods belonging to the genera prevotella, porphyromonas and bacteroides, peptostreptococcus species, mycoplasma hominis, ureaplasma urealyticum, and often mobiluncus species. facultative species of lactobacillus are present in lower concentrations and are less prevalent in bacterial vaginosis t ...	1993	8357043
chorioamnionitis and bacterial vaginosis.	an improved understanding of bacterial vaginosis and of clinical intraamniotic infection and histologic chorioamnionitis has produced data showing strong associations among these conditions. it has recently been shown that the microorganisms in both bacterial vaginosis and clinical intraamniotic infection are similar, of which anaerobes, gardnerella vaginalis, and mycoplasma hominis are the predominant organisms in both. furthermore, in the amniotic fluid of women with intraamniotic infection, s ...	1993	8357045
posthysterectomy wound infection. a review.	we reviewed the microorganisms isolated from gynecologic surgery wounds showing signs of infection, from january to december 1990. a total of 88 samples of abdominal wall exudates from as many patients who had undergone abdominal hysterectomy were studied. in 54 women (61.3%), cultures were positive and, in 26 specimens (48.1%), mixed aerobic-anaerobic flora were isolated. in 28 cases (51.9%), a single microorganism was isolated, the most frequent being staphylococcus aureus, followed by mycopla ...	1993	8359004
[chlamydia and mycoplasma infections in men of couples with involuntary sterility].	there is conflicting evidence on the influence of infections with chlamydia trachomatis and mycoplasma hominis on male and female infertility. we studied the prevalence of chlamydia and mycoplasma in male partners of 165 infertile couples. 25% of couples with tubal and/or andrological sources of infertility showed positive cultures for chlamydia and/or mycoplasma compared with 10% of couples with other causes of infertility. our data suggest, that screening for chlamydia and mycoplasma in infert ...	1993	8375633
erythromycin versus metronidazole in the treatment of bacterial vaginosis.	of 101 women, 15-50 years of age, presenting with vaginal discharge, 34 had bacterial vaginosis and were randomly assigned to a seven-day course of oral treatment with either erythromycin (0.5 g b.i.d.) or metronidazole (0.4 g b.i.d.) in a single-blind, cross-over study. treatment failure (> or = three clinical signs of bacterial vaginosis) occurred in 13 (81%) of 16 patients given erythromycin, as compared with three (17%) of 18 women treated with metronidazole (p < 0.001). persistence of gardn ...	1993	8394627
[do infections with mycoplasma hominis and ureaplasma urealyticum no longer count as sexually transmissible diseases?].		1993	8276597
susceptibilities of mycoplasma hominis, mycoplasma pneumoniae, and ureaplasma urealyticum to a new quinolone, opc 17116.	the susceptibilities of human mycoplasmas to opc 17116 (otsuka america pharmaceutical, inc., rockville, md.) and temafloxacin (abbott laboratories, chicago, ill.) were determined by the agar dilution method and were compared with those to sparfloxacin and ofloxacin. the mics of opc 17116 for 90% of mycoplasma pneumoniae (0.25 microgram/ml) and mycoplasma hominis (0.125 micrograms/ml) isolates tested were closely similar to those of sparfloxacin and were four- to eightfold greater than those of o ...	1993	8215294
genital mycoplasmas revisited--an evaluation of a new culture medium.	the role of genital mycoplasmas as pathogens causing severe neonatal respiratory and central nervous system disease has been highlighted recently following publication of new data. these organisms are generally neglected by diagnostic laboratories in the united kingdom, possibly due to the lack of a suitable commercially-available culture medium. we have evaluated the biomerieux mycoplasma-lyo system using qualitative and quantitative studies and have found it to have suitable qualities and prac ...	1993	8219924
[multicenter study of the in vitro sensitivity of genital mycoplasmas to antibiotics].	the in vitro susceptibility of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) was evaluated in a multicentric study performed in seven hospitals from different geographic areas in france. during a three month period, 324 uu and 72 mh clinical isolates were tested using a system ready for use, sir mycoplasma (sanofi diagnostics pasteur). for uu, the percentage of strains intermediate (i) or resistant (r) was as follows: doxycycline (3), minocycline (2.5), lymecycline (6.7), erythromycin ...	1993	8233624
[a prospective study of mycoplasma infection in a neonatal unit].	ureaplasma urealyticum and mycoplasma hominis were recovered from nasopharyngeal aspirates from 25% of 63 infants admitted to a neonatal unit; this proportion is significantly higher than that seen in a control population of maternity ward babies (0%). birth by cesarean section was associated with a reduced risk of recovery of mycoplasmas. no specific diseases were significantly associated with recovery of mycoplasmas; furthermore, no obstetrical factors were associated with recovery of mycoplas ...	1993	8442648
mycoplasma hominis pneumonia.		1993	8474392
spermicidal and antiviral properties of cholic acid: contraceptive efficacy of a new vaginal sponge (protectaid) containing sodium cholate.	cholic acid (sodium cholate) exhibits a strong spermicidal and antiviral [anti-human immunodeficiency virus (hiv)-1] activity. the same effects are observed for f-5 gel, the active mixture of a new contraceptive sponge (protectaid), which contains sodium cholate in association with low concentrations (0.5%) of nonoxynol-9 and benzalkonium chloride. both cholic acid and the f-5 gel exert a dose-dependent, in-vitro inhibitory effect (i) on the activity of hiv-1 associated reverse transcriptase in ...	1993	7688380
mycoplasma fermentans--hela cell interactions.	a survey of previous evidence for the intracellular localization of mycoplasmas within nonphagocytic cells indicated that it was insufficient to conclude unequivocally that such localization occurred. illustrations of the seemingly intracellular existence of mycoplasma fermentans in the tissues of patients with aids and other patients rekindled interest in the topic of mycoplasmal entrance into epithelial cells. accordingly, the "incognitus" and pg18 strains of m. fermentans were sought and demo ...	1993	7691197
[mycoplasma hominis empyema following pleuropneumonia in late pregnancy].	mycoplasma hominis (m. hominis) frequently colonizes the genital tract, but is rarely isolated from the respiratory tract. dissemination of m. hominis has been documented in women with a febrile illness after delivery. spontaneous recovery is the rule. a 32-year-old woman in the 29th week of pregnancy was hospitalized for pneumonia. she had to be ventilated for respiratory insufficiency and thoracic empyema. the child was delivered by section due to signs of stress in cardiotocography. in the en ...	1993	8272796
wound and perinephric haematomata infection with mycoplasma hominis in a renal transplant recipient.		1993	8159314
successful treatment of mycoplasma mediastinitis after heart-lung transplantation.	mycoplasma hominis and ureaplasma urealyticum are members of the mycoplasma genus that can infect surgical wounds. these organisms are rarely cultured by conventional laboratory techniques, and they respond poorly to commonly used broad-spectrum antibiotics. including this case, five cases of mycoplasma mediastinitis have been reported, three of which followed heart-lung transplantation. successful treatment of mycoplasma mediastinitis after heart-lung transplantation was achieved in this patien ...	1993	8329428
chronic lung disease of preterm infants in finland is not associated with ureaplasma urealyticum colonization.	the occurrence of ureaplasma urealyticum and mycoplasma hominis in the airways and the association of these microorganisms with chronic lung disease was studied in preterm infants with a gestational age less than 30 weeks. tracheal aspirates from 49 preterm infants were cultured; 14 (29%) infants were positive for u. urealyticum, and 1 (2%) was positive for m. hominis. of the 16 patients who developed lung disease, 6 (38%) were positive for u. urealyticum, while the expected number of ureaplasma ...	1993	8477168
in vitro activity of azithromycin against chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis in comparison with erythromycin, roxithromycin and minocycline.	the in vitro activity of azithromycin against 40 strains of chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis was investigated in comparison with erythromycin, roxithromycin and minocycline. all c. trachomatis strains were inhibited by azithromycin at a concentration < or = 0.5 microgram/ml. the initial minimum inhibitory concentration (mic) of the drug for u. urealyticum was 4 microgram/ml, whereas some resistance against the drug was shown by m. hominis. erythromycin and rox ...	1993	8396626
mycoplasma hominis in liver transplantation.		1993	8441972
minocycline compared with doxycycline in the treatment of nongonococcal urethritis and mucopurulent cervicitis.	to compare the efficacy and tolerability of minocycline versus doxycycline in the treatment of nongonococcal urethritis and mucopurulent cervicitis.	1993	8498758
mycoplasmal pericarditis: evidence of invasive disease.	although the pathogenic mycoplasmas usually infect the respiratory and urogenital tracts, these organisms also can cause disease in remote sites. such infections are difficult to diagnose because of both the fastidious nature of the mycoplasmas and the failure to consider their presence. pericarditis is an uncommonly diagnosed and rarely confirmed example of invasive mycoplasmal infection. as part of a prospective study of large pericardial effusions, we discovered two cases with mycoplasma pneu ...	1993	8399939
evaluation of intraspecies genetic variation within the 16s rrna gene of mycoplasma hominis and detection by polymerase chain reaction.	mycoplasma hominis is a heterogeneous species with dna-dna hybridization values ranging from 51 to 100%. we report here the sequencing of the 16s rrna gene of a strain (183) that greatly differs from the type strain (pg21) of this species. comparison of 16s rdna sequences from these two strains showed limited differences, indicating that the two strains belong to the same rrna species complex. using these nucleotide sequence data, we established a rapid method for the detection of m. hominis by ...	1993	7684753
[mycoplasma hominis and ureaplasma urealyticum in pregnant and infertile women. differences in tubal pathology].	we studied the frequency of cervicovaginal bacteria in 47 pregnant women (16-41 weeks of gestational age), in 47 infertile patients and in 4 with gynecological diseases (not infections). when we compared the frequency of bacteria between pregnant and infertile women, we found that the biggest difference was with u. urealyticum. in fact the frequency of this germ was 61% in the total population studied, in pregnant women was of 72% which is statistically higher than the frequency in infertile wom ...	1993	7991855
[the presence of genital mycoplasmas in women of reproductive age].	the authors examined 70 original samples obtained from 70 women seen at the nuevo veranillo community health center because of suspected mycoplasma infection. 23 (33%) of the cultures were positive for mycoplasma. the microscopic and chemical characteristics identified 3 (13%) as ureaplasma urealyticum, 9 (39%) were mycoplasma hominis and 11 (48%) were positive for both organisms. the rate of positive cultures for mycoplasma was high, which could be an important finding since these organisms are ...	1993	8146353
miocamycin. a review of its antimicrobial activity, pharmacokinetic properties and therapeutic potential.	miocamycin is an orally administered 16-membered macrolide antimicrobial drug. it has a spectrum of in vitro activity similar to that of erythromycin, inhibiting a range of gram-positive and gram-negative organisms, atypical microbes and some anaerobes. importantly, miocamycin demonstrates greater in vitro potency than erythromycin against several pathogens including legionella pneumophila, mycoplasma hominis, and ureaplasma urealyticum. equally noteworthy is its activity against erythromycin-re ...	1993	7506653
sequencing analysis reveals a unique gene organization in the gyrb region of mycoplasma hominis.	the homolog of the gyrb gene, which has been reported to be present in the vicinity of the initiation site of replication in bacteria, was mapped on the mycoplasma hominis genome, and the region was subsequently sequenced. five open reading frames were identified flanking the gyrb gene, one of which showed similarity to that which encodes the lica protein of haemophilus influenzae. the organization of the genes in the region showed no resemblance to that in the corresponding regions of other bac ...	1994	7521872
[infected ovarian vein thrombosis in infection with ureaplasma urealyticum and mycoplasma hominis after cesarean section].	puerperal ovarian vein thrombophlebitis (povt) is a rare but severe complication of the post partum period. we report on a patient, who developed a povt based on an infection with ureaplasma urealyticum and mycoplasma hominis. problems of diagnostic procedures in cases of povt and the role of ureaplasma and mycoplasma in infectious obstetric complications are discussed.	1994	8150252
changes in vaginal flora during pregnancy and association with preterm birth.	a prospective study of the vaginal flora changes between midtrimester and labor was undertaken in 560 women; 337 had intact membranes and were in labor when swabs were taken. of these, 45 delivered preterm. gardnerella vaginalis, ureaplasma urealyticum (both associated with preterm birth when present in midtrimester), and mycoplasma hominis commonly persisted between midtrimester and labor, particularly in the preterm cohort (24 [65%] of 37 midtrimester isolates), and these organisms were seldom ...	1994	8077737
analysis of a mycoplasma hominis membrane protein, p120.	the monoclonal antibody mab 26.7d generated against a clinical isolate of mycoplasma hominis 7488 was shown to react with a surface-exposed epitope on a 120-kda protein (p120). the gene encoding the protein was cloned and sequenced, and the transcriptional start point was determined by primer extension analysis. the gene contained an open reading frame of 3237 bp encoding a peptide of 1079 amino acids with a deduced molecular mass of 123 kda. a putative amino-terminal signal peptide and cleavage ...	1994	8082822
expectant management of premature rupture of membranes with amniotic fluid cultures positive for ureaplasma urealyticum alone.	our purpose was to determine maternal and neonatal outcome in patients with premature rupture of membranes and amniotic fluid cultures positive for ureaplasma urealyticum alone.	1994	8116718
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.	the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...	1994	8126176
detection of chlamydia trachomatis antigen & toxoplasma gondii (igm) & mycoplasma hominis (igg) antibodies by elisa in women with bad obstetric history.	a total of 365 non pregnant women with bad obstetrics history (boh) were studied with a control group of 100 women who had delivered full term clinically normal infants. the presence of c. trachomatis antigen and t. gondii (igm) and m. hominis (igg) antibodies was assessed by elisa test. c. trachomatis antigen was detected in 28.2 per cent of women with boh whereas t. gondii and m. hominis specific antibodies were found in 43.83 and 27.1 per cent respectively, these were highly significant (p < ...	1994	7927547
prevalence of genital chlamydia and mycoplasma infection in couples attending a male infertility clinic.	to investigate the prevalence of genital mycoplasma (ureaplasma urealyticum and mycoplasma hominis) and chlamydia trachomatis infections in infertile and fertile couples, 135 infertile couples from a male infertility clinic and 88 fertile couples from a prenatal care clinic were randomly screened. semen, urethral and cervical smears were cultured for genital mycoplasma and chlamydia. antichlamydia iga, igg and igm antibodies were tested in blood and semen. although the overall prevalence of urea ...	1994	7957794
in vitro antimycoplasmal activities of rufloxacin and its metabolite mf 922.	the in vitro activities of rufloxacin and its metabolite, mf 922, were compared with those of ofloxacin, ciprofloxacin, erythromycin, and minocycline against mycoplasma pneumoniae, mycoplasma hominis, mycoplasma fermentans, and ureaplasma urealyticum. rufloxacin, mf 922, and ciprofloxacin shared similar activities against all mycoplasmas tested. (mics for 90% of isolates tested [mic90s], 0.5 to 4 micrograms/ml. ofloxacin had the lowest mic90s for u. urealyticum, m. fermentans, and m. hominis (mi ...	1994	7872762
mycoplasma hominis septic arthritis: two case reports and review.	mycoplasma hominis is normally a commensal of humans. when the organism is pathogenic, it primarily causes disease in the genitourinary tract. septic arthritis caused by m. hominis is a rare condition that occurs chiefly in the postpartum period, in immunosuppressed hosts, or in patients who have recently undergone urinary tract manipulation. arthritis caused by m. hominis is clinically indistinguishable from septic arthritis caused by other bacteria. diagnosis is often delayed because infection ...	1994	7888535
prevalence and influence of mycoplasma hominis and ureaplasma urealyticum in 218 african pregnant women and their infants.	to estimate the prevalence of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) in the lower genital tract of pregnant women, their evolution during pregnancy, and the effect of these pathogens on the outcome of pregnancy in equatorial africa.	1994	7805974
mycoplasma hominis abscess secondary to respiratory tract infection.		1994	7806885
[genital microbial flora in a population of high obstetrical risk].	there is increasing evidence showing the association between maternal genital infection, prematurity and neonatal or maternal morbidity. to know the genital flora of women attending a high risk pregnancy service, 73 consecutive pregnant women attending the high risk pregnancy service at the hospital san borja-arriarán from may to august 1992 were included in this study, 75.3% of the vaginal discharges corresponded to fluids of normal appearance and 24.7% were altered. the microorganisms most fre ...	1994	7809430
mycoplasma hominis in pelvic inflammatory disease.	fifty one cases of pelvic inflammatory disease (pid) and sixty one control subjects were screened for the genital mycoplasmas. cervical swabs, urine & blood were collected from the patients and the control subjects and were processed by standard techniques for isolation and identification of bacteria, fungi and mycoplasmas. the rate of isolation of m. hominis from the pid and controls were 54.90% and 16.39% respectively, and this difference was statistically significant (p. < 0.001). metabolic i ...	1994	7814061
genital flora in pregnancy and its association with intrauterine growth retardation.	a study of risk factors for intrauterine growth retardation (iugr) was conducted among a cohort of 13,914 pregnant women enrolled in the multicenter vaginal infections and prematurity study. from 23 through 26 weeks of gestational age, cultures of specimens from the vagina and cervix were done for group b streptococci, neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, candida albicans, gardnerella vaginalis, mycoplasma hominis, ureaplasma urealyticum, and anaerobic gram-negati ...	1994	7814541
a longitudinal study of bacterial vaginosis during pregnancy.	to determine the longitudinal changes in the incidence of vaginosis in pregnancy.	1994	7826957