Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antibacterial peptides derived from caprine whey proteins, by digestion with human gastrointestinal juice. | peptides in caprine whey were identified after in vitro digestion with human gastrointestinal enzymes in order to determine their antibacterial effect. the digestion was performed in two continuing steps using human gastric juice (ph 2·5) and human duodenal juice (ph 8) at 37°c. after digestion the hydrolysate was fractionated and 106 peptides were identified. from these results, twenty-two peptides, located in the protein molecules, were synthesised and antibacterial activity examined. strong a ... | 2011 | 21554806 |
| antioxidant, antihypertensive and antimicrobial properties of ovine milk caseinate hydrolyzed with a microbial protease. | background: bioactive peptides might be released from precursor proteins through enzymatic hydrolysis. these molecules could be potentially employed in health and food products. in this investigation, ovine milk caseinate hydrolysates obtained with a novel microbial protease derived from bacillus sp. p7 were evaluated for antioxidant, antimicrobial, and angiotensin i-converting enzyme (ace)-inhibitory activities. results: antioxidant activity measured by the 2,2'-azino-bis-(3-ethylbenzothiazolin ... | 2011 | 21560133 |
| characterization of bacilli isolated from the confined environments of the antarctic concordia station and the international space station. | abstract bacillus and related genera comprise opportunist and pathogen species that can threaten the health of a crew in confined stations required for long-term missions. in this study, 43 bacilli from confined environments, that is, the antarctic concordia station and the international space station, were characterized in terms of virulence and plasmid exchange potentials. no specific virulence feature, such as the production of toxins or unusual antibiotic resistance, was detected. most of th ... | 2011 | 21563959 |
| follow-up of the bacillus cereus emetic toxin production in penne pasta under household conditions using liquid chromatography coupled with mass spectrometry. | two outbreak-related bacillus cereus emetic strains were investigated for their growth and cereulide production potential in penne pasta at 4, 8 and 25 °c during 7-day storage. cereulide production was detected and quantified by lc-ms method (lod of 1 ng/ml, loq of 5 ng/ml) and growth was determined by culture-based enumeration. inoculated b. cereus strains (10(5) cfu/g) were able to reach counts of more than 10(8) cfu/g and cereulide production of about 500 ng/g already after 3 days of storage ... | 2011 | 21569959 |
| isolation of glucosidase and phospholipase positive bacillus circulans on aloa medium. | aim: to report the growth of glucosidase and phospholipase positive bacteria on agar listeria according to ottaviani and agosti (aloa) different from listeria monocytogenes, listeria ivanovii and bacillus cereus. methods and results: raw water-buffalo milk was analysed according to en iso 11290. streaking of fraser broth on aloa resulted in green colonies with an opaque halo after 48 h at 30°c. colonies were transferred onto tryptone soya yeast extract agar and showed cultural characteristics ... | 2011 | 21575023 |
| bacillus cereus g9241 makes anthrax toxin and capsule like highly virulent b. anthracis ames but behaves like attenuated toxigenic nonencapsulated b. anthracis sterne in rabbits and mice. | bacillus cereus g9241 was isolated from a welder with a pulmonary anthrax-like illness. the organism contains two megaplasmids, pbcxo1 and pbc218. these plasmids are analogous to the b. anthracis ames plasmids pxo1 and pxo2 that encode anthrax toxins and capsule, respectively. here we evaluated the virulence of b. cereus g9241 as well as the contributions of pbcxo1 and pbc218 to virulence. b. cereus g9241 was avirulent in new zealand rabbits after subcutaneous inoculation and attenuated 100-fold ... | 2011 | 21576337 |
| successful surgical drainage and aggressive medical therapy in a preterm neonate with bacillus cereus meningitis. | bacillus cereus meningitis is a rare disease with a very high mortality rate in neonates. the authors present the rare case of a premature infant with b. cereus bacteremia and subsequent intracranial abscesses. in addition to aggressive medical therapy, surgical drainage was performed via a left frontal mini-craniotomy. at 15 months of age, the patient had mild developmental delay, cortical blindness, and sensorineural hearing loss. the clinical case is described and difficulties in the manageme ... | 2011 | 21577046 |
| physiological parameters of bacillus cereus marking the end of acid-induced lag phases. | during lag phases microbial cells adapt to their environment and prepare to proliferate. physiological parameters of b. cereus cells upon exposure to near-growth-boundary acid stress were investigated and markers for the transition between lag phase and growth were identified using fluorescent probes combined with flow cytometry. determination of cell counts and optical density revealed lag phases of 1h, 2h and 5h, in cultures shifted to ph 7, ph 5.3 (set with lactic acid) and ph 4.9 (set with s ... | 2011 | 21592605 |
| synthesis of gold and silver nanoparticles using purified urak. | this study aims at developing a new eco-friendly process for the synthesis of silver nanoparticles (agnps) and gold nanoparticles (aunps) using purified urak. urak is a fibrinolytic enzyme produced by bacillus cereus nk1. the enzyme was purified and used for the synthesis of aunps and agnps. the enzyme produced agnps when incubated with 1mm agno3 for 24h and aunps when incubated with 1mm haucl4 for 60h. but when naoh was added, the synthesis was rapid and occurred within 5min for agnps and 12h f ... | 2011 | 21592748 |
| sulfamethoxazole biodegradation and biotransformation in the water-sediment system of a natural river. | in this study, the biodegradation of sulfamethoxazole (smx) as affected by temperature, humic acid (ha) and smx concentrations was investigated by hplc-ms/ms analysis based on water-sediment batch experiments. the first order decay model (c=c(0)×exp (-kt)) was best fitted for smx biodegradation. smx degradation significantly increased with elevated temperature (degradation rate was 82.9% at 25°c vs. 40.5% at 4°c in sediment), ha contents (30mg/l of ha facilitated smx degradation rate at 90.1% vs ... | 2011 | 21596556 |
| monitoring the wet-heat inactivation dynamics of single spores of bacillus species using raman tweezers, differential interference contrast and nucleic acid dye fluorescence microscopy. | dynamic processes during wet heat treatment at 80-90°c of individual spores of bacillus cereus, bacillus megaterium and bacillus subtilis were investigated using dual-trap raman spectroscopy, differential interference contrast (dic) microscopy and nucleic acid stain (syto 16) fluorescence microscopy. during spore wet heat treatment, while spores' 1:1 chelate of ca(2+) with dipicolinic acid (cadpa) was released rapidly at a highly variable time t(lag), the levels of spore nucleic acids remained n ... | 2011 | 21602365 |
| enzymatic synthesis of isopropyl acetate by immobilized bacillus cereus lipase in organic medium. | selective production of fragrance fatty acid ester from isopropanol and acetic acid has been achieved using silica-immobilized lipase of bacillus cereus mtcc 8372. a purified thermoalkalophilic extracellular lipase was immobilized by adsorption onto the silica. the effects of various parameters like molar ratio of substrates (isopropanol and acetic acid; 25 to 100 mm), concentration of biocatalyst (25-125 mg/ml), reaction time, reaction temperature, organic solvents, molecular sieves, and initia ... | 2011 | 21603222 |
| screening of australian plants for antimicrobial activity against campylobacter jejuni. | campylobacter jejuni is the most common cause of acute enteritis in humans, with symptoms such as diarrhoea, fever and abdominal cramps. in this study, 115 extracts from 109 australian plant species were investigated for their antimicrobial activities against two c. jejuni strains using an in vitro broth microdilution assay. among the plants tested, 107 (93%) extracts showed activity at a concentration between 32 and 1024 µg/ml against at least one c. jejuni strain. seventeen plant extracts were ... | 2011 | 21604309 |
| [screening endophytic bacteria against plant-parasitic nematodes]. | plant-parasite nematode is one of the most important pathogens in plant. our objective is to screen endophytic bacteria against plant-parasitic nematodes from plant. | 2011 | 21604551 |
| antibacterial activity of chemically defined chitosans: influence of molecular weight, degree of acetylation and test organism. | chitosans, polysaccharides obtained from the exoskeleton of crustaceans, have been shown to exert antibacterial activity in vitro and their use as a food preservative is of growing interest. however, beyond a consensus that chitosan appears to disrupt the bacterial cell membrane, published data are inconsistent on the chemical characteristics that confer the antibacterial activity of chitosan. while most authors agree that the net charge density of the polymer (reflected in the fraction of posit ... | 2011 | 21605923 |
| efficacy of vitrectomy in improving the outcome of bacillus cereus endophthalmitis. | purpose:: to evaluate the efficacy of vitrectomy with vancomycin for the treatment of experimental bacillus cereus endophthalmitis. methods:: endophthalmitis was initiated in rabbits via intravitreal injection of 100 colony-forming unit b. cereus. treatment groups included 25-gauge transconjunctival sutureless vitrectomy with intravitreal vancomycin (1 mg) or vancomycin alone. groups were treated at 4, 5, or 6 hours after infection. at 48 hours (for 4-hour and 5-hour groups) or 36 hours (for the ... | 2011 | 21606892 |
| mutually exclusive distribution of the sap and eag s-layer genes and the lytb/lyta cell wall hydrolase genes in bacillus thuringiensis. | recently, two bacillus thuringiensis strains were reported to synthesize parasporal inclusion bodies made not of the expected crystal (cry) proteins but rather of the surface layer proteins (slp) sap (encoded by sap) and ea1 (encoded by eag), respectively. whether the presence of the sap and eag genes is restricted to these two b. thuringiensis strains or ubiquitous in b. thuringiensis is unknown. we report here the distribution of the sap and eag genes in b. thuringiensis. strains in the bacill ... | 2011 | 21611767 |
| bacterial load of fresh vegetables and their resistance to the currently used antibiotics in saudi arabia. | abstract this study was carried out to describe the bacterial load and the occurrence of some disease-causing enteric bacteria on raw vegetables sold in saudi markets. the study further aimed to analyze antibiotic resistance rates, production of extended-spectrum beta lactamase, and plasmid carriage among bacterial population of raw vegetables. results revealed that none of them contained bacillus cereus, salmonella, and escherichia coli o157:h7. however, staphylococcus aureus and shigella were ... | 2011 | 21612423 |
| antifungal quinazolinones from marine-derived bacillus cereus and their preparation. | two new quinazolinones alkaloids, r(+)-2-(heptan-3-yl)quinazolin-4(3h)-one (1) and (2r,3'r)+(2s,3'r)-2-(heptan-3-yl)-2,3-dihydroquinazolin-4(1h)-one (2) (a pair of epimers), as well as seven known analogues, 2-methylquinazolin-4(3h)-one (3), 2-benzylquinazolin-4(3h)-one (4), cyclo-(pro-ile), cyclo-(pro-leu), cyclo-(pro-val), cyclo-(pro-phe), and cyclo-(tyr-pro) were isolated from the n-butyl alcohol extract of the marine-derived bacterium bacillus cereus 041381. the new compounds were identified ... | 2011 | 21612927 |
| some like it hot: the effects of climate change on reproduction, immune function and disease resistance in the cricket gryllus texensis. | in many parts of the world, climate change is increasing the frequency and severity of heat waves. how do heat waves impact short-lived poikilotherms such as insects? in the cricket, gryllus texensis, 6 days of elevated temperatures (i.e. 7°c above the average field temperature and 5°c above their preferred temperature) resulted in increased egg laying, faster egg development and greater mass gain. the increased temperature also increased activity of phenoloxidase and lysozyme-like enzymes, two ... | 2011 | 21613515 |
| natural resistance of rose petals to microbial attack. | petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ... | 2011 | 21614712 |
| preparation of mucoadhesive oral patches containing tetracycline hydrochloride and carvacrol for treatment of local mouth bacterial infections and candidiasis. | the specific aim of this work was to prepare mucoadhesive patches containing tetracycline hydrochloride and carvacrol in an attempt to develop a novel oral drug delivery system for the treatment of mouth infections. the bilayered patches were prepared using ethyl cellulose as a backing layer and carbopol 934 as a matrix mucoadhesive layer. patches were prepared with different loading amounts of tetracycline hydrochloride and carvacrol. the antimicrobial activity was assessed for the prepared pat ... | 2011 | 21617783 |
| molecular weight change of polyhydroxyalkanoate (pha) caused by the phac subunit of pha synthase from bacillus cereus yb-4 in recombinant escherichia coli. | polyhydroxyalkanoate (pha)-producing bacillus strains possess class iv pha synthases composed of two subunit types, namely, phar and phac. in the present study, pha synthases from bacillus megaterium nbrc15308(t) (pharc(bm)), b. cereus yb-4 (pharc(yb4)), and hybrids (phar(bm)c(yb4) and phar(yb4)c(bm)) were expressed in escherichia coli jm109 to characterize the molecular weight of the synthesized poly(3-hydroxybutyrate) [p(3hb)]. pharc(bm) synthesized p(3hb) with a relatively high molecular weig ... | 2011 | 21618968 |
| reference genes for quantitative, reverse-transcription pcr in bacillus cereus group strains throughout the bacterial life cycle. | quantitative reverse-transcription pcr (rt-qpcr) has become a major tool to better understand the biology and pathogenesis of bacteria. one prerequisite of valid rt-qpcr data is their proper normalization to stably expressed reference genes. to identify and evaluate reference genes suitable for normalization of gene expression data in bacillus cereus group strains, mrna levels of eleven candidate reference genes (rpsu, nifu, udp (udp-n-acetylglucosamine 2-epimerase), bt9727_5154/bc_5475, bt9727_ ... | 2011 | 21620905 |
| role played by exosporium glycoproteins in the surface properties of bacillus cereus spores and in their adhesion properties to stainless steel. | b. cereus spores are surrounded by a loose fitting layer, called the exosporium, whose distal part is mainly formed of glycoproteins. the role played by the exosporium glycoproteins of b. cereus atcc 14579 (bcla, exsh) was investigated considering hydrophobicity and charge, as well as adhesion properties of spores to stainless steel. the absence of bcla increased both the isoelectric point (iep) and hydrophobicity of whole spores, whilst simultaneously reducing the interaction between spores and ... | 2011 | 21622795 |
| phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth. | a local herb, kacip fatimah, is famous amongst malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of labisia pumila benth. total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. leaves of var. pumi ... | 2011 | 21623314 |
| examining the genetic variation of reference microbial cultures used within food and environmental laboratories using fluorescent amplified fragment length polymorphism analysis. | fluorescent amplified fragment length polymorphism (faflp) analysis was applied to genetically fingerprint 'working culture control strains' used by accredited food microbiology laboratories. a working culture control strain is defined as a sub-culture from a strain initially obtained from an authenticated source (such as the national collection of type cultures, nctc), that is maintained for use with routine testing within the laboratory. working culture control strains from eight food examinat ... | 2011 | 21623896 |
| a rapd-pcr method for the rapid detection of bacillus cereus. | distinction of bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. from our previous work on rapd profiles of bacilli, we found a possibility that b. cereus strains could be distinguished from other bacilli. in this work, rapd-pcr profiles of b. cereus strains were obtained using a 10-mer (s30) as a primer, and a b. cereus specific 0.91-kb band was produced from all tested strains. the rapd-pcr procedure also successfully detected ... | 2011 | 21464598 |
| rapid identification of emetic bacillus cereus by immunochromatography. | the immunochromatographic assay, which targets a marker protein co-expressed during the synthesis of cereulide by an emetic bacillus cereus strain, was used for easily, rapidly and specifically identifying the emetic strains among b. cereus strains from various materials associated with food poisonings. all 50 of the emetic strains showed a positive reaction to the assay, but all 50 diarrheal strains had a negative reaction. the bacterial counts of 108 cfu/ml in enrichment broth and 109 cfu/ml i ... | 2011 | 21467628 |
| crystal structure of a phosphorylation-coupled saccharide transporter. | saccharides have a central role in the nutrition of all living organisms. whereas several saccharide uptake systems are shared between the different phylogenetic kingdoms, the phosphoenolpyruvate-dependent phosphotransferase system exists almost exclusively in bacteria. this multi-component system includes an integral membrane protein eiic that transports saccharides and assists in their phosphorylation. here we present the crystal structure of an eiic from bacillus cereus that transports diacet ... | 2011 | 21471968 |
| resistance and biosorption mechanism of silver ions by bacillus cereus biomass. | biosorption of silver ions onto bacillus cereus biomass was investigated. overall kinetic experiments were performed for the determination of the necessary contact time for the attainment of equilibrium. it was found that the overall biosorption process was best described by pseudo second-order kinetic model. the crystals detected by scanning electron microscope and x-ray photoelectron spectroscopy suggested the precipitation was a possible mechanism of biosorption. the molecular genetics of sil ... | 2011 | 21476348 |
| phenolic composition and antimicrobial and antiquorum sensing activity of an ethanolic extract of peels from the apple cultivar annurca. | abstract this study investigated the phenolic composition and antioxidant capacity of an ethanolic extract from the peel of the annurca (malus domestica var. annurca), which is widespread in the campania region of southern italy. the antimicrobial effect of the extract on different pathogens was also examined. the potential antiquorum sensing activity of the extract was tested by using the microorganism chromobacterium violaceum. ultra-performance liquid chromatography revealed that rutin, epica ... | 2011 | 21476926 |
| growth characteristics and development of a predictive model for bacillus cereus in fresh wet noodles with added ethanol and thiamine. | response surface methodology was used to determine growth characteristics and to develop a predictive model to describe specific growth rates of bacillus cereus in wet noodles containing a combination of ethanol (0 to 2% [vol/wt]) and vitamin b(1) (0 to 2 g/liter). b. cereus f4810/72, which produces an emetic toxin, was used in this study. the noodles containing b. cereus were incubated at 10°c. the growth curves were fitted to the modified gompertz equation using nonlinear regression, and the g ... | 2011 | 21477484 |
| [epidemiological investigation for outbreak of food poisoning caused by bacillus cereus among the workers at a local company in 2010]. | in july 2 2010, a diarrhea outbreak occurred among the workers in a company in gyeungju city, korea. an epidemiological investigation was performed to clarify the cause and transmission route of the outbreak. | 2011 | 21483225 |
| purification and characterization of a new bacillus thuringiensis bacteriocin active against listeria monocytogenes, bacillus cereus and agrobacterium tumefaciens. | this study reports on the identification, characterization and purification of a new bacteriocin, named bacthuricin f103, from a bacillus thuringiensis strain bupm103. bacthuricin f103 production began in the early exponential phase and reached a maximum in the middle of the same phase. two chromatographic methods based on high performance liquid chromatography and fast protein liquid chromatography systems were used to purify bacthuricin f103. sodium dodecyl sulphate-polyacrylamide gel electrop ... | 2011 | 21487734 |
| antimicrobial activity of blumea balsamifera (lin.) dc. extracts and essential oil. | leaves of blumea balsamifera (lin.) dc. are used in traditional thai and chinese medicine for the treatment of septic wounds and other infections. in this study, the essential oil, hexane, dichloromethane and methanol extracts of these leaves were evaluated for antibacterial and antifungal activities using the disc diffusion assay and agar microdilution method. the essential oil was the most potent, with a minimum inhibitory concentration (mic) of 150 µg ml(-1) against bacillus cereus and an mic ... | 2011 | 21500092 |
| the proof of the pudding is in the eating: an outbreak of emetic syndrome after a kindergarten excursion, berlin, germany, december 2007. | an outbreak of food poisoning (emetic syndrome) occurred in three kindergartens (a, b and c) in berlin, germany, on 3 december 2007 after an excursion during which food was served. we conducted a retrospective cohort study among the kindergarten children and personnel who participated in the trip. the overall attack rate among the 155 participants was 30%. it was 31% among the 137 children (aged two to six years) and 17% among adults (n=18). the consumption of rice pudding was significantly asso ... | 2011 | 21507320 |
| risk evaluation and management to reaching a suggested fso in a steam meal. | steam meals are ready-to-eat meals composed of raw and semi-cooked ingredients, which get cooked while microwave heating. in this study, an indian style meal was selected, chicken tandoori, from two different producers. these meals were first evaluated with the risk ranger® to identify the main foodborne pathogens risks, which were listeria monocytogenes, salmonella typhimurium and bacillus cereus. thereafter, quantitative microbiology was applied using different models and verified with growth ... | 2011 | 21511122 |
| the effects of glutaraldehyde on the control of single and dual biofilms of bacillus cereus and pseudomonas fluorescens. | glutaraldehyde (glut) was evaluated for control of single and dual species biofilms of bacillus cereus and pseudomonas fluorescens on stainless steel surfaces using a chemostat system. the biofilms were characterized in terms of mass, cell density, total and matrix proteins and polysaccharides. the control action of glut was assessed in terms of inactivation and removal of biofilm. post-biocide action was characterized 3, 7, 12, 24, 48 and 72 h after treatment. tests with planktonic cells were a ... | 2011 | 21512918 |
| crystal structure of pduo-type atp:cob(i)alamin adenosyltransferase from bacillus cereus in a complex with atp. | atp:cobalamin adenosyltransferases catalyze the transfer a 5'-deoxyadenosyl moiety from atp to cob(i)alamin in the synthesis of the co-c bond of coenzyme b(12). there are three types of adenosyltransferases, coba, pduo and eutt. among these adenosyltransferases, the pduo-type adenosyltransferases is the most widely distributed enzyme. structural comparisons between apo bcpduo and bcpduo in complex with mgatp revealed that the n-terminal strands of both structures were ordered, which is in contra ... | 2011 | 21514283 |
| antibacterial activity of different honeys against pathogenic bacteria. | to study the antimicrobial activity of honey, 60 samples of various botanical origin were evaluated for their antimicrobial activities against 16 clinical pathogens and their respective reference strains. the microbiological quality of honeys and the antibiotic susceptibility of the various isolates were also examined. the bioassay applied for determining the antimicrobial effect employs the well-agar diffusion method and the estimation of minimum active dilution which produces a 1mm diameter in ... | 2011 | 21524711 |
| effect of benzoic acid and combination of benzoic acid with a probiotic containing bacillus cereus var. toyoi in weaned pig nutrition. | the purpose of this study was to assess the efficacy of a probiotic containing bacillus cereus var. toyoi spores (toyocerin) and benzoic acid (vevovitall) on growth performance and diarrhoea in weaning pigs, against negative controls. the trial groups were as follows: (a) nc group (negative controls): no treatment (b) toyo group: same feed as in the controls plus toyocerin at a dose of 1 x 10(9) bacillus cereus var. toyoi spores/kg feed, (c) ba group: same feed as in the controls plus vevovitall ... | 2011 | 21528721 |
| the nudix hydrolase cdp-chase, a cdp-choline pyrophosphatase, is an asymmetric dimer with two distinct enzymatic activities. | a nudix enzyme from bacillus cereus (accession no. np_831800) catalyzes the hydrolysis of cdp-choline to produce cmp and phosphocholine. here we show that in addition, the enzyme has a 3'→5' rna exonuclease activity. the structure of the free enzyme, determined to 1.8 å resolution, shows that the enzyme is an asymmetric dimer. each monomer consists of two domains, an n-terminal helical domain and a c-terminal nudix domain. the n-terminal domain is placed relative to the c-terminal domain in such ... | 2011 | 21531795 |
| synthesis, antibacterial activity and quantum-chemical studies of novel 2-arylidenehydrazinyl-4-arylthiazole analogues. | a new series of 2-arylidenehydrazinyl-4-arylthiazole derivatives (2a-k) was designed and synthesized through a rapid, simple, and efficient methodology in excellent isolated yield. these compounds were screened for in vitro antimicrobial activities against eight bacteria, e.g. bacillus cereus, staphylococcus aureus, bacillus subtilis, bacillus megaterium, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhi, escherichia coli, and three fungi e.g. aspergillus oryzae, candida albicans, a ... | 2011 | 21532194 |
| synthesis, characterization and in vitro antimicrobial evaluation of new compounds incorporating oxindole nucleus. | new compounds incorporating with the oxindole nucleus were synthesized via the reaction of substituted isatins [5-methyl-, 5-chloro- and 1-hydroxymethyl isatins] with different nucleophiles. the structures of the newly compounds were elucidated on the basis of ftir, (1)h nmr, (13)cmr spectral data, gc/ms and chemical analysis. investigation of antimicrobial activity of the new compounds was evaluated using broth dilution technique in terms of minimal inhibitory concentration (mic) count against ... | 2011 | 21534861 |
| effect of olive powder and high hydrostatic pressure on the inactivation of bacillus cereus spores in a reference medium. | the aim of this work was to study the effect of olive powder combined with high hydrostatic pressure (hhp) on the inactivation of bacillus cereus spores, to use it as an additional control hurdle in beverages pasteurised by this technology. with this purpose, reference medium prepared at different concentrations of olive powder was inoculated with b. cereus spores and subjected to different pressure treatments. the outgrowth capacity of the treated spores was then determined at 20-¦c and 32-¦c. ... | 2011 | 21381901 |
| emetic disease caused by bacillus cereus after consumption of tuna fish in a beach club. | several vomiting episodes were reported in december 2007 by the management of a beach club in calvi+á. the subsequent case investigation confirmed tuna fish consumption a few hours before onset of emesis in all cases. microbiological analyses detected high bacterial levels in ready-to-eat fish samples, indicating inappropriate cooking procedures. more important, elevated levels of bacillus cereus were present both in raw and cooked fish. no other pathogens were detected, indicating b. cereus as ... | 2011 | 21381943 |
| maklamicin, an antibacterial polyketide from an endophytic micromonospora sp. | a new spirotetronate-class polyketide, maklamicin (1), was isolated from the culture extract of an endophytic actinomycete of the genus micromonospora. the structure and relative configuration of 1 were elucidated by interpretation of nmr and other spectroscopic data, and the absolute configuration was determined using the modified mosher method. maklamicin (1) showed strong to modest antimicrobial activity against gram-positive bacteria. | 2011 | 21388191 |
| partial characterization of bacteriocins produced by two lactobacilus strains with probiotic properties. | the probiotic characteristics of lactobacillus brevis bg18 and lb. plantarum bg33, isolated from traditional turkish tulum cheese were assessed. these two bacteriocinproducer strains exhibited good probiotic characteristics such as resistance in media containing 0.3% bile salt, pepsin (3 mg mlôü+-¦), and pancreatine (1 mg mlôü+-¦) as well as acid resistance at ph 2. they were also adhered to caco-2 epithelial cells in a manner comparable to escherichia coli lmg3083 (etec) and salmonella typhimur ... | 2011 | 21388923 |
| improving the ethanol yield by reducing glycerol formation using cofactor regulation in saccharomyces cerevisiae. | to increase ethanol yield and decrease glycerol production in saccharomyces cerevisiae, the strategies of direct cofactor-regulation were explored. during anaerobic batch fermentations, the yeast expressing bacillus cereus gapn gene, encoding non-phosphorylating nadp(+)-dependent glyceraldehyde-3-phosphate dehydrognease, produced 73.8 g ethanol l(-1), corresponding to 96% of theoretical maximum yield compared to 92% for the wild type. the yeast expressing escherichia coli frda gene encoding the ... | 2011 | 21400237 |
| effectiveness of chitosan against mature biofilms formed by food related bacteria. | chitosan has proven antimicrobial properties against planktonic cell growth. little is known, however, about its effects on already established biofilms. oriented for application in food industry disinfection, the effectiveness of both medium molecular weight (mmw) chitosan and its enzymatically hydrolyzed product was tested against mature biofilms of four pathogenic strains, listeria monocytogenes, bacillus cereus, staphylococcus aureus and salmonella enterica, and a food spoilage species, pseu ... | 2011 | 21340015 |
| synthesis and structure of [(?(6)-p-cymene)ru(2-anthracen-9-ylmethylene-n-ethylhydrazinecarbothioamide)cl]cl; biological evaluation, topoisomerase ii inhibition and reaction with dna and human serum albumin. | we have synthesized and evaluated the biological properties of a compound of the type [?(6)-p-cymene)ru(etatsc)cl]cl (1) where etatsc = 2-anthracen-9-ylmethylene-n-ethylhydrazinecarbothioamide, a thiosemicarbazone. the complex has been characterized by elemental analysis, spectroscopically (nmr, uv-vis, and ir) and structurally by xrd. the in vitro anticancer activity of 1 has been evaluated against two human colon cancer cell lines. the ic(50) value for activity against hct-116 was 224 ± 7 µm a ... | 2011 | 21347491 |
| characterization of a novel temperate phage originating from a cereulide-producing bacillus cereus strain. | a novel temperate bacteriophage was isolated from a bacillus cereus cereulide-producing strain and named vb_bces-iebh. vb_bces-iebh belongs to the siphoviridae family. the complete genome sequence (53 kb) was determined and annotated. eighty-seven orfs were detected and for 28, a putative function was assigned using the aclame database. vb_bces-iebh replicates as a plasmid in the prophage state. accordingly, a 9-kb plasmid-like region composed of 13 orfs was identified. a fragment of around 2000 ... | 2011 | 21349326 |
| hot water surface pasteurisation of lamb carcasses: microbial effects and cost-benefit considerations. | although hot water pasteurisation of carcasses is accepted as a general intervention in usa, this is not the case in europe. the aims of this study were (i) to evaluate the microbiological effects of hot water pasteurisation of lamb carcasses, both after slaughtering and dressing and following subsequent chilling and storage; (ii) to discuss hot water pasteurisation from a public health and cost-benefit perspective; (iii) to discuss the benefits of hot water pasteurisation compared with use of s ... | 2011 | 21356564 |
| comparative analysis of antimicrobial activities of valinomycin and cereulide, the bacillus cereus emetic toxin. | cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. cereulide, produced by members of the bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. a comparative analysis of the antimicrobial activity of valinomycin produced by streptomyces spp. and cereulide was performed at a ph range of ph 5.5 to ph 9.5, under anaerobic and aerobic conditions. both compounds display ph-dependent activity agai ... | 2011 | 21357430 |
| pathogen detection using a liquid array technology. | low concentrations of microbial pathogens in pure and mixed samples were detected using a bead-based, liquid array technology. a 20-bp sequence in the 23s rrna gene, rrl, was amplified in four microorganisms: bacillus cereus, escherichia coli, salmonella enterica and staphylococcus aureus. pcr products were positively identified with the luminex(®) 100™ system. the system could detect very low amounts of dna and the instrument response was proportional to the input concentration. the lower limit ... | 2011 | 21361936 |
| substrate specificity of spoiiga, a signal-transducing aspartic protease in bacilli. | spoiiga is a novel type of membrane-associated aspartic protease that responds to a signal from the forespore by cleaving pro-s(e) in the mother cell during sporulation of bacillus subtilis. very little is known about how spoiiga recognizes pro-s(e). by co-expressing proteins in escherichia coli, it was shown that charge reversal substitutions for acidic residues 24 and 25 of pro-s(e), and for basic residues 245 and 284 of spoiiga, impaired cleavage. these results are consistent with a model pre ... | 2011 | 21362630 |
| multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in bacillus cereus batch cultures. | based on two staining protocols, dioc(6)(3)/propidium iodide (pi) and redoxsensor green (an indicator of bacterial reductase activity)/pi, multi-parameter flow cytometry and cell sorting has identified at least four distinguishable physiological states during batch cultures of bacillus cereus. furthermore, dependent on the position in the growth curve, single cells gave rise to varying numbers of colonies when sorted individually onto nutrient agar plates. these growing colonies derived from a s ... | 2011 | 21365289 |
| osmoadaptative accumulation of n?-acetyl-ß-lysine in green sulfur bacteria and bacillus cereus cect 148t. | the compatible solute n(?)-acetyl-ß-lysine (neabl), thus far considered unique to methanogenic archaea, has been found to accumulate in several strains of green sulfur bacteria (gsb) and bacillus cereus cect 148(t) under salt stress. a similar mixture of compatible solutes including trehalose, a-glutamate, ß-glutamate and neabl has been detected in salt-tolerant gsb strains of different phylogenetic branches. the ability of b. cereus to synthesize this compound was predicted from available genom ... | 2011 | 21371089 |
| two capsular polysaccharides enable bacillus cereus g9241 to cause anthrax-like disease. | bacillus cereus g9241 causes an anthrax-like respiratory illness in humans; however, the molecular mechanisms of disease pathogenesis are not known. genome sequencing identified two putative virulence plasmids proposed to provide for anthrax toxin (pbcxo1) and/or capsule expression (pbc218). we report here that b. cereus g9241 causes anthrax-like disease in immune-competent mice, which is dependent on each of the two virulence plasmids. pbcxo1 encodes paga1, the homologue of anthrax protective a ... | 2011 | 21371137 |
| comparing the mannitol-egg yolk-polymyxin agar plating method with the three-tube most-probable-number method for enumeration of bacillus cereus spores in raw and high-temperature, short-time pasteurized milk. | the u.s. food and drug administration's bacteriological analytical manual recommends two enumeration methods for bacillus cereus: (i) standard plate count method with mannitol-egg yolk-polymyxin (myp) agar and (ii) a most-probable-number (mpn) method with tryptic soy broth (tsb) supplemented with 0.1% polymyxin sulfate. this study compared the effectiveness of myp and mpn methods for detecting and enumerating b. cereus in raw and high-temperature, short-time pasteurized skim (0.5%), 2%, and whol ... | 2011 | 21375885 |
| the plant growth-promoting rhizobacterium bacillus cereus ar156 induces systemic resistance in arabidopsis thaliana by simultaneously activating salicylate- and jasmonate/ethylene-dependent signaling pathways. | bacillus cereus ar156 is a plant growth-promoting rhizobacterium that induces resistance against a broad spectrum of pathogens including pseudomonas syringae pv. tomato dc3000. this study analyzed ar156-induced systemic resistance (isr) to dc3000 in arabidopsis ecotype col-0 plants. compared with mock-treated plants, ar156-treated ones showed an increase in biomass and reductions in disease severity and pathogen density in the leaves. the defense-related genes pr1, pr2, pr5, and pdf1.2 were conc ... | 2011 | 21198361 |
| synergistic effects of sodium hypochlorite and ultraviolet radiation in reducing the levels of selected foodborne pathogenic bacteria. | the purpose of this study was to determine whether combined treatment would produce synergistic effects to facilitate the sterilization of food products during production relative to single treatment. to assess this hypothesis, we investigated the bactericidal effects of ultraviolet (uv) irradiation and a commercial chemical disinfectant, sodium hypochlorite (naclo), on bacillus cereus f4810/72, cronobacter sakazakii kctc 2949, staphylococcus aureus atcc 35556, escherichia coli atcc 10536, and s ... | 2011 | 21204702 |
| optimization of bioassay for tetracycline detection in milk by means of chemometric techniques. | in this study, a microbiological method of dichotomous response using bacillus cereus was designed and optimized to detect tetracyclines (tcs) at concentrations near to the maximum residue limits (mrls). | 2011 | 21204876 |
| metallo-β-lactamase-catalyzed hydrolysis of cephalosporins: some mechanistic insights into the effect of heterocyclic thiones on enzyme activity. | the hydrolysis of β-lactam antibiotics using zinc-containing metallo-β-lactamases (mβl) is one of the major bacterial defense systems. these enzymes can catalyze the hydrolysis of a variety of antibiotics including the latest generation of cephalosporins, cephamycins, and imipenem. it is shown in this paper that the cephalosporins having heterocyclic -sr side chains are less prone to mβl-mediated hydrolysis than the antibiotics that do not have such side chains. this is partly due to the inhibit ... | 2011 | 21210647 |
| the effect of lemongrass oil and its major components on clinical isolate mastitis pathogens and their mechanisms of action on staphylococcus aureus dmst 4745. | the aims of this study were to investigate the antibacterial activity of lemongrass oil (lg) and its major components which were citral, geraniol and myrcene, against four strains of clinically isolated bovine mastitis pathogens, including staphylococcus aureus, streptococcus agalactiae, bacillus cereus and escherichia coli by the broth microdilution method, as well as their activity on s. aureus biofilm formation. attempts to clarify their mechanisms of action by investigation of the effects on ... | 2011 | 21316719 |
| inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (mangifera indica l.). | this study investigated the antimicrobial activities and modes of action of penta-, hexa-, hepta-, octa-, nona-, and deca-o-galloylglucose (gallotannins) isolated from mango kernels. the mics and minimum bactericidal concentrations (mbcs) against food-borne bacteria and fungi were determined using a critical dilution assay. gram-positive bacteria were generally more susceptible to gallotannins than were gram-negative bacteria. the mics of gallotannins against bacillus subtilis, bacillus cereus, ... | 2011 | 21317249 |
| isolation and characterization of cold-active family viii esterases from an arctic soil metagenome. | functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, estm-n1 and estm-n2, from a metagenomic dna library of arctic soil samples. estm-n1 and estm-n2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class c β-lactamases. however, they shared a relatively low level of sequence similarity (30%) with each other. phylogenetic analysis of bacterial lipolytic enzymes confirmed that estm-n1 ... | 2011 | 21318360 |
| phenotypic & genetic characterization of bacillus cereus isolated from the acute diarrhoeal patients. | bacillus cereus is one of the pathogens responsible for human diarrhoea, mainly due to consumption of contaminated food. the present study was undertaken to determine the occurrence of b. cereus among diarrhoeal patients and its phenotypic and genetic characteristics that determine the virulence and clonal features. | 2011 | 21321425 |
| a flagellin-producing lactococcus strain: interactions with mucin and enteropathogens. | bacillus cereus ch is a probiotic strain used in human nutrition whose adhesion to mucin is dependent on its surface-associated flagellin. flagellins from the surface of several probiotic bacillus strains were efficiently extracted with 5 m licl and identified by peptide fingerprinting. based on the proteomic analysis, cloning of the gene coding for the flagellin of b. cereus ch was performed in the lactococcal vector pnz8110 under the control of a nisin-inducible promoter. the resulting strain, ... | 2011 | 21323981 |
| [fatal bacillus cereus sepsis and rhabdomyolysis in a patient with hodgkin's disease]. | 2011 | 21324561 | |
| meta-analysis of endophthalmitis after intravitreal injection of anti-vascular endothelial growth factor agents: causative organisms and possible prevention strategies. | : to report the rates of endophthalmitis and the spectrum of causative organisms after intravitreal injection of anti-vascular endothelial growth factor agents and possible prevention strategies. | 2011 | 21330939 |
| current physical and sds extraction methods do not efficiently remove exosporium proteins from bacillus anthracis spores. | biochemical studies of the outermost spore layers of the bacillus cereus family are hindered by difficulties in efficient dispersal of the external spore layers and difficulties in dissociating protein complexes that comprise the exosporium layer. detergent and physical methods have been utilized to disrupt the exosporium layer. herein we compare commonly used sds extraction buffers used to extract spore proteins and demonstrate the incomplete extractability of the exosporium layer by these meth ... | 2011 | 21338631 |
| synergistic antibacterial activity of the essential oil of cuminum cyminum l. seed and nisin in a food model. | aims: to investigate effects of various concentrations of the essential oil of cuminum cyminum l. seed alone and in combination with nisin on survival of vegetative forms of bacillus cereus and bacillus subtilis in a food model (commercial barley soup) and their ultrastructure. methods and results: gas chromatography-mass spectrometry analysis indicated that cumin aldehyde (29·02%) and α-terpinen-7-al (20·70%) constituted the highest amount of the essential oil. the lowest concentration of the ... | 2011 | 21226797 |
| the effects of korean propolis against foodborne pathogens and transmission electron microscopic examination. | this study was performed to evaluate the effects of korean propolis against foodborne pathogens and spores of bacillus cereus and to investigate the antimicrobial activity against b. cereus structure by transmission electron microscopy (tem). the antimicrobial effects of the korean propolis were tested against foodborne pathogens including gram-positive (b. cereus, listeria monocytogenes and staphylococcus aureus) and gram-negative (salmonella typhimurium, escherichia coli and pseudomonas fluore ... | 2011 | 21232643 |
| carijodienone from the octocoral carijoa multiflora. a spiropregnane-based steroid. | two new steroids (1 and 2) and the known pregna-1,4,20-trien-3-one (3) have been isolated from the pacific octocoral carijoa multiflora. compound 1 possesses a novel spiropregnane-based steroidal skeleton. the photochemical transformation of 3 into 1 allowed the assignment of the absolute configuration at c-10 of 1. the antibacterial activities of compounds 1 and 3 were evaluated against a panel of bacterial strains. | 2011 | 21235217 |
| isolation and identification of soil bacteria growing at the expense of arbuscular mycorrhizal fungi. | soil-microorganism symbioses are of fundamental importance for plant adaptation to the environment. research in microbial ecology has revealed that some soil bacteria are associated with arbuscular mycorrhizal fungi (amf). however, these interactions may be much more complex than originally thought. to assess the type of bacteria associated with amf, we initially isolated spores of glomus irregulare from an agrostis stolonifera rhizosphere. the spores were washed with sterile water and plated on ... | 2011 | 21219415 |
| development of oscillation method for reducing foodborne pathogens on lettuce and spinach. | in this study, the efficacy of an oscillator for reducing the numbers of foodborne pathogens on lettuce and spinach was tested. a cocktail of three strains each of salmonella typhimurium, escherichia coli o157:h7 and listeria monocytogenes cells and of bacillus cereus spores was inoculated onto lettuce and spinach leaves and followed by oscillation at 10 hz and 20 hz for up to 30s. after treatment of inoculated lettuce leaf with an oscillator at 20 hz for 30s, 2.58, 2.82, 2.21 and 2.22 log(10)cf ... | 2011 | 21269718 |
| analysis of bacterial communities of traditional fermented west african cereal foods using culture independent methods. | in this study, the microbial composition of kunu-zaki and ogi, two popular foods in nigeria produced after natural, uncontrolled fermentation of cereals, was assessed by culture-independent molecular profiling methods. in particular, pcr-denaturing gradient gel electrophoresis and construction of 16s rrna gene clone libraries revealed the presence of diverse bacterial communities. dna sequencing of the highly variable v3 region of the 16s rrna genes obtained from pcr-dgge fingerprints identified ... | 2011 | 21272950 |
| using an insect model to assess correlation between temperature and virulence in bacillus weihenstephanensis and bacillus cereus. | the closely related bacterial species bacillus cereus and bacillus weihenstephanensis are adapted to the mesophilic and the psychrotrophic temperature range, respectively. while b. cereus strains are associated with foodborne diseases, b. weihenstephanensis strains are so far not, although similar virulence genes are found in both species. our investigations show that both species were virulent in the insect model, galleria mellonella, following infection via oral and haemocoel routes. however, ... | 2011 | 21276046 |
| antifungal activity displayed by cereulide, the emetic toxin produced by bacillus cereus. | in this study, the fungistatic activity of bacillus cereus cereulide-producing strains was demonstrated against nine fungal species. the role of cereulide was confirmed using plasmid-cured derivatives and ces knockout mutants. the fungistatic spectra of cereulide and valinomycin, a chemically related cyclododecadepsipeptide, were also compared and found to be similar but distinct. | 2011 | 21278263 |
| impact of sorbic acid on germinant receptor-dependent and -independent germination pathways in bacillus cereus. | amino acid- and inosine-induced germination of bacillus cereus atcc 14579 spores was reversibly inhibited in the presence of 3 mm undissociated sorbic acid. exposure to high hydrostatic pressure, ca-dipicolinic acid (dpa), and bryostatin, an activator of prkc kinase, negated this inhibition, pointing to specific blockage of signal transduction in germinant receptor-mediated germination. | 2011 | 21278268 |
| green pigment from bacillus cereus m(1)(16) (mtcc 5521): production parameters and antibacterial activity. | a bacterial strain, bacillus cereus m(1)(16) (mtcc 5521), isolated and identified in our laboratory produces a green pigment when grown in nutrient broth at stationary condition. optimum fermentation parameters for maximum pigment production are ph 7.0, temperature 30°c, time of incubation 72 h and inoculum volume 1% from 20 h grown cell suspension. magnesium ion enhances pigment production whereas calcium and zinc ions inhibit the process. the pigment is better extracted from the fermented brot ... | 2011 | 21279697 |
| biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. | in developing countries like india, there are many industrial areas discharging effluent containing large amount of polyaromatic hydrocarbon (pah) which causes hazardous effect on the soil-water environment. the objective of this study was to isolate and characterize high-efficiency pah-degrading microbial consortium from 3 decade old petrochemical refinery field located in nagpur, maharashtra with history of pah disposal. based on biochemical tests and 16s rdna gene sequence analysis the consor ... | 2011 | 21281999 |
| preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles. | the fabrication of carvacrol-loaded chitosan nanoparticles was achieved by a two-step method, i.e., oil-in-water emulsion and ionic gelation of chitosan with pentasodium tripolyphosphate. the obtained particles possessed encapsulation efficiency (ee) and loading capacity (lc) in the ranges of 14-31% and 3-21%, respectively, when the initial carvacrol content was 0.25-1.25 g/g of chitosan. the individual particles exhibited a spherical shape with an average diameter of 40-80 nm, and a positively ... | 2011 | 21296562 |
| lactate dehydrogenase a promotes communication between carbohydrate catabolism and virulence in bacillus cereus. | the diarrheal potential of a bacillus cereus strain is essentially dictated by the amount of secreted nonhemolytic enterotoxin (nhe). expression of genes encoding nhe is regulated by several factors, including the metabolic state of the cells. to identify metabolic sensors that could promote communication between central metabolism and nhe expression, we compared four strains of the b. cereus group in terms of metabolic and nhe expression capacities. we performed growth performance measurements, ... | 2011 | 21296961 |
| isolation and characterization of phages infecting bacillus cereus. | to isolate and characterize bacteriophages (phages) that infect the foodborne pathogen bacillus cereus. | 2011 | 21299576 |
| cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract. | cadmium (cd²(+)), a naturally occurring heavy metal, is an important environmental pollutant and a potent toxicant to bacteria. the gastrointestinal (gi) tract microflora has a marked capacity to cope with the increased load of ingested metals. however, heavy metals may have harmful effects on git microflora. under the conditions of experimental exposure to cadmium, changes in the population of intestinal microflora in healthy mice were examined. five experimental groups received 23 to 50 mg kg⁻ ... | 2011 | 20488844 |
| a multiplex real-time pcr for identifying and differentiating b. anthracis virulent types. | bacillus anthracis is closely related to the endospore forming bacteria bacillus cereus and bacillus thuringiensis. for accurate detection of the life threatening pathogen b. anthracis, it is essential to distinguish between these three species. here we present a novel multiplex real-time pcr for simultaneous specific identification of b. anthracis and discrimination of different b. anthracis virulence types. specific b. anthracis markers were selected by whole genome comparison and different se ... | 2011 | 20826037 |
| alkaline protease from bacillus cereus vitsn04: potential application as a dehairing agent. | the objective of this work is to use protease enzyme as an ecofriendly alternative to chemicals in dehairing. an alkaline protease producing bacterium was isolated from protein-rich soil sample. the bacterium was identified as bacillus cereus vitsn04 by 16s rrna gene sequencing method. growth characteristics and protease activity were studied in yeast, malt, beef, nutrient broth and soybean casein digest media and the enzyme secretion was found to correspond with growth. maximum protease product ... | 2011 | 20937566 |
| triplex pcr-based detection of enterotoxigenic bacillus cereus atcc 14579 in nonfat dry milk. | although many strains of bacillaceae are considered nonpathogenic, bacillus cereus is recognized worldwide as a bacterial pathogen in a variety of foods. the ability of b. cereus to cause gastroenteritis following ingestion of contaminated food is due to the production of enterotoxins. the ubiquity of this genus makes it a persistent problem for quality assurance in food processing environments. the primary objective of this study was to develop and apply a multiplex real-time pcr-based assay fo ... | 2011 | 20586065 |
| haemolysin ii is a bacillus cereus virulence factor that induces apoptosis of macrophages. | bacillus cereus is a gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. these infections are characterized by bacterial accumulation despite the recruitment of phagocytic cells. the precise mechanisms and the bacterial factors allowing b. cereus to circumvent host immune responses remain to be elucidated. we have previously shown that b. cereus induces macrophage cell death by an unknown mechanism. here we identified the toxic component from the b. ... | 2011 | 20731668 |
| evaluation of bacillus strains as model systems for the work on bacillus anthracis spores. | available strain collections of bacillus anthracis and bacillus cereus were screened for b. cereus strains sharing major genotypic characteristics with b. anthracis. based on the comparison of partial spoiiiab sequences, whole genome sequences and mlst, a strain set representing different lineages including candidate model strains for b. anthracis was compiled. spores from the selected strain set and two b. anthracis strains were prepared according to a newly optimized protocol transferable to b ... | 2011 | 20800917 |
| restriction analysis and partial sequencing of the 16s rrna gene as index for rapid identification of bacillus species. | restriction fragment length analysis of pcr amplified 16s rdna with alui revealed the presence of a 265 bp fragment in all species of bacillus with the exception of b. cereus and b. thuringiensis, which contains two restriction sites within this fragment which results in three smaller fragments totalling to 265 bp. some distant species of bacillus with no evidence of this fragment could be delineated into other genera based on phenotypic and genotypic parameters. blast search for homologous sequ ... | 2011 | 20694833 |
| [investigation of ampicillin effect on morphological and mechanical properties of escherichia coli and bacillus cereus cells with atomic force microscopy]. | the effect of subbacteriostatic concentrations of ampicillin on morphological and mechanical properties of gramnegative and grampositive cells of escherichia coli k12 tg1 and bacillus cereus ip 5832 respectively was studied with atomic force microscopy. significant heterogeneity of the bacterial populations was shown by the character of the response to the antibiotic effect. the common feature was increase of the cell size likely due to the effect of the inner osmotic pressure on the lowered cel ... | 2011 | 22359862 |
| evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based pcr genomic fingerprinting. | bacillus thuringiensis is classified into serovars on the basis of h-flagellar antigens. several alternative typing methods have been described. among them, a b. cereus group-specific repetitive extragenic palindromic (rep)-pcr fingerprinting technique was shown to be discriminative and able to identify b. thuringiensis serovars. the aim of this study was to investigate the genomic diversity and relationship among b. thuringiensis strains collected from different argentinean ecosystems. thirty-s ... | 2011 | 22286045 |
| multifunctional zno/nylon 6 nanofiber mats by an electrospinning-electrospraying hybrid process for use in protective applications. | zno/nylon 6 nanofiber mats were prepared by an electrospinning-electrospraying hybrid process in which zno nanoparticles were dispersed on the surface of nylon 6 nanofibers without becoming completely embedded. the prepared zno/nylon 6 nanofiber mats were evaluated for their abilities to kill bacteria or inhibit their growth and to catalytically detoxify chemicals. results showed that these zno/nylon 6 nanofiber mats had excellent antibacterial efficiency (99.99%) against both the gram-negative ... | 2011 | 27877442 |
| isolation and characterization of bacillus cereus ist105 from electroplating effluent for detoxification of hexavalent chromium. | electroplating industries are the main sources of heavy metals, chromium, nickel, lead, zinc, cadmium and copper. the highest concentrations of chromium (vi) in the effluent cause a direct hazards to human and animals. therefore, there is a need of an effective and affordable biotechnological solution for removal of chromium from electroplating effluent. | 2011 | 22351260 |
| isolation and growth characteristics of chromium(vi) and pentachlorophenol tolerant bacterial isolate from treated tannery effluent for its possible use in simultaneous bioremediation. | the bacterial strains resistant to pentachlorophenol (pcp) and hexavalent chromium [cr(vi)] were isolated from treated tannery effluent of a common effluent treatment plant. most of the physico-chemical parameters analyzed were above permissible limits. thirty-eight and four bacterial isolates, respectively were found resistant to >50 μg/ml concentration of [cr(vi)] and the same level of pcp. out of the above 42 isolates, only one was found simultaneously tolerant to higher levels of both pcp (5 ... | 2011 | 22282630 |
| characterization of two metal resistant bacillus strains isolated from slag disposal site at burnpur, india. | two strains of bacillus sp. resistant to arsenate and lead designated as assp9 and pbsp6, respectively were isolated from the slag disposal site. they were identified to be related to bacillus cereus cluster on the basis of 16s rdna based sequence analysis and phenotypic characteristics. both were rod-shaped (assp9, 2-5 microm and pbsp6, 2-4 microm), aerobic, salt tolerant (2-8% naci), endospore forming bacteria with minor differences like the assp9 showed sporangial bulging and pbsp6 had positi ... | 2011 | 22471215 |