Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the genome of bovine herpesvirus 1 (bhv-1) strains exhibiting a neuropathogenic potential compared to known bhv-1 strains by restriction site mapping and cross-hybridization. | bovine herpesvirus 1 (bhv-1) strains can be differentiated by their dna and polypeptide patterns, and by antigenic properties as demonstrated by monoclonal antibodies. we classified the bhv-1 strains according to these data as bhv-1.1, bhv-1.2 (a/b) and bhv-1.3 (a/b). bhv-1.1 and bhv-1.2 correspond to the well known 'common' bhv-1 strains, whereas bhv-1.3 has only recently been recognized and exhibits a neuropathogenic potential. in the present paper we describe the structural genome characteris ... | 1986 | 3026111 |
| restriction endonuclease patterns of bovine herpesvirus type 1 isolated from bovine mammary glands. | restriction endonuclease analysis was used, in conjunction with viral neutralization and growth-curve experiments, to compare a bovine herpesvirus type 1 (bhv-1) isolate, originally obtained from bovine mammary gland lesions, with a standard bhv-1 strain, infectious bovine rhinotracheitis virus. although differences were not detected by viral neutralization or growth-curve experiments, restriction fragment patterns generated by bam hi, eco ri, hind iii, and hpa i, revealed definite differences b ... | 1986 | 3026212 |
| diagnosis of bovine respiratory syncytial virus infections improved by virus detection in lung lavage samples. | the potential of lung lavage to diagnose and study infections with bovine respiratory syncytial virus (brsv) was investigated. the technique was simple to perform under field conditions, and even in calves with respiratory tract disease, serious drawbacks were not observed. in 9 epizootics of respiratory tract disease, brsv infection was diagnosed in 1 or more calves. the brsv was detected from a total of 21 of 32 calves by immunofluorescence in cells recovered from lavage fluids, and the virus ... | 1986 | 3511803 |
| study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. | bovine respiratory syncytial virus (brsv) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. a microtiter serum-virus neutralization test proved to be the best means of identifying involvement of brsv; seroconversion (fourfold or greater rise in titer) was demonstrated in 10 of the 14 epizootics. only limited involvement of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and bovine adenovirus type 3 was recogni ... | 1986 | 3733504 |
| colonization of the nasal passages of calves with pasteurella haemolytica serotype 1 and regeneration of colonization after experimentally induced viral infection of the respiratory tract. | healthy nonstressed calves were inoculated intranasally with or subjected to aerosol exposure to pasteurella haemolytica serotype 1. only 4 of 28 calves harbored the bacterium in enough numbers to be isolated from the nasal passages for more than 7 days. after apparent clearing from the nasal passages, 8 calves were inoculated intranasally with infectious bovine rhinotracheitis virus; 2 of these calves shed the p haemolytica during clinical illness due to the virus. the remaining 20 calves were ... | 1986 | 3752678 |
| cytotoxicity of bovine lymphocytes after treatment with lymphokines. | cytotoxic lymphocytes were generated from bovine peripheral blood mononuclear leukocytes after in vitro stimulation with lymphokines that contained interleukin-2. lymphokine-stimulated cultures were cytotoxic to k562 cells (human natural killer [nk] targets) and yac-1 cells (mouse nk targets), but not to hsb-2 cells (human nk targets) in a 4-hour, 51cr-release assay. cells generated after lymphokine activation also mediated antibody-dependent cellular cytotoxicity to hsb-2 cells. appearance of e ... | 1986 | 3488701 |
| bovine peripheral blood leukocyte subpopulation dynamics following a primary bovine herpesvirus-1 infection. | population dynamics of bovine peripheral blood leukocyte subpopulations were quantitated following a primary bovine herpesvirus-1 (bhv-1) infection. percoll isolated peripheral blood mononuclear cell (pbmc) subpopulations were analyzed using flow cytometry (fc) and cytochemical stains. between days two to eight post-infection (pi) there was a significant decrease in the percentage of t-cells and nont/nonb cells which was accompanied by an increased percentage of b-cells and monocytes. these perc ... | 1987 | 3509948 |
| t lymphocyte population dynamics and function following a primary bovine herpesvirus type-1 infection. | following a primary bovine herpesvirus-1 (bhv-1) infection the concanavalin a (con a) induced proliferative responses of peripheral blood t lymphocytes were suppressed. this suppression occurred in the absence of detectible serum suppressor factors, suppressor cell activity or decreased accessory cell function. however, regression analysis demonstrated a significant correlation between the percentage of t lymphocytes present within the peripheral blood mononuclear cell (pbmc) population and the ... | 1987 | 3509949 |
| efficacy of a streptomycin-dependent, live pasteurella haemolytica vaccine against challenge exposure to pasteurella haemolytica in cattle. | a streptomycin-dependent, live pasteurella haemolytica vaccine was given in 1 or 2 doses to 2 groups of weaned calves; 2 other groups of calves were not vaccinated. all calves in the vaccinated groups and calves in 1 of the nonvaccinated groups were stressed by transport, intratracheally inoculated with bovine herpesvirus type-1 (cooper strain), and then intratracheally inoculated with p haemolytica type a1. the 4th group of calves (nonvaccinated controls) was not stressed and were not intratrac ... | 1987 | 3296883 |
| vaccination studies against experimental bovine pasteurella pneumonia. | vaccination-challenge experiments were conducted in colostrum-deprived calves to evaluate the efficacy of pasteurella bacterins and vaccines against experimental pneumonic pasteurellosis. calves were vaccinated with formalin-killed bacterins and live vaccines, then challenge exposed intratracheally with p. haemolytica or p. multocida. infectious bovine rhinotracheitis virus was inoculated intranasally three to four days prior to p. haemolytica challenge-exposure. all calves were examined for mac ... | 1987 | 3300921 |
| pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. | nasal passages of calves with a virus-induced respiratory tract disease became colonized by pasteurella haemolytica serotype 1 after they were inoculated intranasally with p haemolytica. inoculation with infectious bovine rhinotracheitis virus caused a more severe clinical illness and resulted in a greater degree of colonization with p haemolytica than developed after inoculation with parainfluenza-3 virus. nasal passages of parainfluenza-3 virus-inoculated calves were colonized to a greater deg ... | 1987 | 2829666 |
| generation of il-2 dependent bovine cytotoxic t lymphocyte clones reactive against bhv-1 infected target cells: loss of genetic restriction and virus specificity. | to gain insight into the mechanisms of immunity to bovine herpesvirus type-1 (bhv-1) in particular the importance of the t-cell response, we attempted to clone bovine cytotoxic t lymphocytes that were specific for bhv-1 infected autologous target cells. a number of bovine t cell clones were generated by limiting dilution in the presence of bovine recombinant il-2 and bhv-1 infected target cells as feeder layers. these clones were maintained in culture on crude il-2 containing supernatants. in fu ... | 1987 | 2855701 |
| serologic survey of viral antibodies in the peruvian alpaca (lama pacos). | sera from more than 100 alpacas (lama pacos) from the peruvian southern sierra were examined for antibodies to 8 viruses known to infect other domestic animals. on the basis of these serologic findings and previously published serologic or clinical data, it is now known that the alpaca can be infected with the following viruses: parainfluenza-3, bovine respiratory syncytial virus, bovine herpesvirus-1, bluetongue virus, border disease virus, influenza a virus, rotavirus, rabies virus, vesicular ... | 1987 | 3826854 |
| effects of infectious bovine rhinotracheitis virus infection on bovine airway reactivity. | the response of isolated tracheal and bronchial strips to isoproterenol in vitro was studied in eleven male jersey calves. clinical, microbiological and pathological evaluations of the calves were carried out. in calves exposed once or twice to infectious bovine rhinotracheitis virus, the relaxation threshold of the trachealis muscle to isoproterenol was significantly (p less than 0.05) impaired (threshold 5.0 x 10(-7) m, single exposure and 1.0 x 10(-7) m, double exposure), when compared with u ... | 1987 | 3651887 |
| effect of dexamethasone on bovine leukocyte functions and bovine herpesvirus type-1 replication. | in an attempt to elucidate the mechanism whereby dexamethasone could reactivate bovine herpesvirus type-1 the effect of dexamethasone on virus replication and leukocyte functions was assessed. no effect was detectable on either virus yield or in vitro replication kinetics. in contrast, dexamethasone influenced several leukocyte functions thought to be of importance in antiviral defense and maintenance of latency. in vitro exposure of peripheral blood polymorphonuclear neutrophilic granulocytes o ... | 1987 | 3651888 |
| early embryonic death in heifers after inoculation with bovine herpesvirus-1 and reactivation of latent virus in reproductive tissues. | thirteen crossbred heifers seronegative for bovine herpesvirus-1 (bhv-1) were bred naturally to a seronegative bull. eight heifers were inoculated with bhv-1, iv, on postbreeding day (pbd) 7 or 14. viremia was detected in heifers 1 through 7, and virus also was isolated from nasal and vaginal secretions of heifers 2, 3, 4, 6, and 7. the pregnancy status of all heifers was monitored from pbd 14 to pbd 35 by determining plasma progesterone concentrations at 1- to 3-day intervals. decreased progest ... | 1987 | 3434897 |
| comparative efficacy of three 2'-fluoropyrimidine nucleosides and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (bw b759u) against pseudorabies and equine rhinopneumonitis virus infection in vitro and in laboratory animals. | the three 2'-fluoropyrimidine nucleosides 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), showed high activity in rk13 monolayers against equine rhinopneumonitis virus, (ehv-1, ic50 range 0.02-0.18 microm), aujeszky's disease virus (shv-1, pseudorabies, ic50 range 0.25-7 microm) and infectious bovine rhinotracheitis virus (1br, bhv-1, ic50 ... | 1987 | 3026244 |
| intracellular localization and transport of three different bovine herpesvirus type 1 glycoproteins involved in neutralization. | monoclonal antibodies against 3 different glycoproteins of bovine herpesvirus type 1 (bhv-1) involved in virus neutralization were used in indirect immunofluorescence (iif) tests to characterize the appearance and transport to the plasma membrane of virus antigens in the infected cells. antibodies against gp 117 and gp 71 glycoproteins first showed pronounced ring-like nuclear fluorescence at 4 hours post-infection (pi), followed by staining of the perinuclear region, presumably the golgi appara ... | 1987 | 3026288 |
| natural cell-mediated cytotoxicity to cells infected with infectious bovine rhinotracheitis virus. | cell-mediated cytotoxicity against viral-infected cells was demonstrated in a 6-hr 51cr release assay. peripheral blood mononuclear leukocytes from both infectious bovine rhinotracheitis virus (ibrv)-infected and noninfected cattle exhibited preferential lysis against ibrv-infected primary bovine embryonic kidney (bek) cells compared to cells infected with pseudorabies virus and noninfected bek cells. addition of specific antibody to the assay did not enhance cytotoxicity. the effector cell was ... | 1987 | 3029944 |
| inheritance and interaction of immune traits in beef calves. | three sets of blood samples were obtained from beef calves of two experimental populations and assayed for various immunological measurements. the first set of samples was taken between 24 and 48 h after birth and quantified for igg1 concentration. a second set was taken immediately prior to vaccination for infectious bovine rhinotracheitis virus (ibrv; at an average age of 164 d) and a third set taken 60 d post-vaccination. these later samples were quantified for antibodies specific to ibrv. le ... | 1987 | 3030992 |
| hemagglutination inhibition and virus neutralizing response of rabbits inoculated with bovine herpesvirus 1 subunit vaccine. | the immunogenicity of bovine herpesvirus type 1 (bhv-1) hemagglutinin has been investigated. both live and nonionic detergent solubilized vaccines were prepared and 5000 hemagglutinating units (hau) were injected subcutaneously into rabbits. both types of vaccine induced a good antibody response but live virus was four times more efficient in inducing hemagglutination inhibiting and neutralizing antibodies than either triton x-100- or octylglucoside-solubilized subunit vaccine. blotting analysis ... | 1987 | 3031871 |
| highly sensitive antigen detection procedures for the diagnosis of infectious bovine rhinotracheitis: amplified elisa and reverse passive haemagglutination. | the sensitivity of an enzyme-linked immunosorbent assay (elisa) for the detection of bovid herpesvirus 1 antigen was increased by up to 50-fold using the biotin-avidin interaction to amplify the reaction, when compared with a simple sandwich elisa. an alternative immunoassay, reverse passive haemagglutination (rpha), had a similar sensitivity to the amplified elisa, and was technically simpler to perform. both the amplified elisa and the rpha could detect viral antigen in the nasal secretions of ... | 1987 | 3031873 |
| reactivation in calves of latent infection by bovid herpesvirus-4. | nine calves, six of which had been infected with strain 85/bh 16tv and three with strain 85/bh 232tn of bovid herpesvirus-4 (bhv-4), were treated with dexamethasone (dms) three months after infection. dms administration did not induce any clinical signs of disease, but bhv-4 was isolated from the nasal swabbings of all calves for a maximum of 8 days after the start of dms treatment. the virus was also isolated from the nerve tissues, nasal mucosa, lymph nodes, lung and spleen of 4 calves that we ... | 1987 | 3033450 |
| reactivity of monoclonal antibodies to proteins of a neurotropic bovine herpesvirus 1 (bhv-1) strain and to proteins of representative bhv-1 strains. | 15 monoclonal antibodies (mcabs) were induced with the neuropathogenic strain n 569 of bovine herpesvirus 1 type 3 (bhv-1.3). nine of them could be shown by radioimmunoprecipitation assay to react with viral glycoproteins and two of these mcabs were able to neutralize strain n 569. the reactivity of these 15 monoclonals was compared with 11 monoclonal antibodies induced with a bhv-1.1 strain. the available monoclonal antibodies made it possible to characterize bhv-1.3 and to classify bhv-1 into ... | 1987 | 3034198 |
| reactivation of infectious bovine rhinotracheitis virus by transport. | transport was studied as a cause of reactivation of infectious bovine rhinotracheitis virus (bovine herpesvirus-1; bhv-1) in heifers vaccinated 2-6 months before transport, using a double dose of the thermosensitive (ts) vaccine strain (tracherine). eight out of 19 animals showed ts strain re-excretion over a period of 1-3 days, beginning, in 5 out of the 8 heifers, the day after transport. in 14 other heifers, only sera were examined by sero-neutralisation: only 1 out of these 14 animals showed ... | 1987 | 3034502 |
| comparison of stationary and roller cultures for the isolation of herpesviruses affecting livestock. | the effect of rotating cultures on the isolation and cultivation of pseudorabies, infectious bovine rhinotracheitis and equine rhinopneumonitis viruses was studied. serial ten-fold dilutions of laboratory strains of these viruses and field strains of pseudorabies virus were inoculated in two each of roller and stationary tubes of appropriate cell cultures. all tubes were examined daily for the appearance of cytopathic effects. roller cultures were found to slightly enhance the speed and extent o ... | 1987 | 3035348 |
| viral-bacterial pneumonia in calves: effects on plasma eicosanoids and long chain fatty acids. | in the pathogenesis of bovine pneumonic pasteurellosis, immunodepression induced by stress or respiratory viral infection permits superinfection of the lungs with pasteurella hemolytica, which results in exudative fibrinous pneumonia. therefore, bovine pneumonic pasteurellosis was induced by sequential inoculations of calves with bovine herpes virus-1 (bhv-1, 3 x 10(7) tissue culture infectious dose 50 (tcid50)/nostril), followed 3 days later by challenge with p. hemolytica (15 x 10(9) colony-fo ... | 1987 | 3036736 |
| the genome of caprine herpesvirus 1: genome structure and relatedness to bovine herpesvirus 1. | caprine herpesvirus 1 (caphv-1) dna was examined by electron microscopy, restriction site mapping and homology studies with bovine herpesvirus 1 (bhv-1) dna. although the restriction site maps differed significantly, we showed that the genome structures of caphv-1 and bhv-1 were identical and that the dnas shared a high degree of base sequence homology. | 1987 | 3037021 |
| protection of cattle from bovine herpesvirus type i (bhv-1) infection by immunization with individual viral glycoproteins. | the major glycoproteins gi, giii, and giv of bovine herpesvirus-1 (bhv-1) were found to induce high levels of antibody in cattle which could neutralize virus and participate in antibody-dependent cell cytotoxicity of bhv-1-infected cells. immunized animals were fully protected from disease, using a bhv-1/pasteurella haemolytica aerosol challenge model but not from infection with the virus. thus, virus could still replicate in the nasal passages of immunized animals, although to a lesser extent t ... | 1987 | 3037783 |
| detection of bovine herpesvirus-1 nucleic acid sequences, using a dot-blot hybridization procedure. | a sensitive and specific procedure for the detection of argentine isolates of bovine herpesvirus-1 was developed. the procedure was based on a dot-blot, nucleic acid hybridization, using 32p, nick-translated, plasmidic probes. the probes contained cloned bam h1 restriction fragments in the left half of the viral genome. the detection limit of the procedure was 10 pg of viral dna. | 1987 | 3037953 |
| use of recombinant bovine alpha 1 interferon in reducing respiratory disease induced by bovine herpesvirus type 1. | intranasal or intramuscular treatment of calves with recombinant bovine alpha 1 interferon before challenge with bovine herpesvirus type 1 and pasteurella haemolytica reduced clinical signs, number of sick days, lung lesions, and weight loss. the effective dose was determined to be relatively broad within the range of 1 to 50 mg per animal. no adverse effects were observed even at high doses of interferon (50 mg per animal). administration before virus infection was more effective than administr ... | 1987 | 3038008 |
| elisa for bovine herpesvirus 1 (bhv1) antibody with hirt supernatant. | detailed studies were made of the ability of hirt supernatant (hs) from bovine herpesvirus 1 (bhv1) infected cultures to sensitize plates for enzyme-linked immunosorbent assay (elisa). the ultracentrifuged pellet of hs had less sensitizing activity than the supernatant, but the antigen was removed completely by 0.22 micron filters and to some extent by 0.45 micron filters; it was minimally affected by sonication; it was destroyed by the action of pronase but not by dnaase i when a kallikrein ina ... | 1987 | 3038916 |
| [bhv-1 infection of cattle: stabilization by active immunization of seropositive animals with inactivated vaccine--a field trial]. | 1987 | 3040371 | |
| comparison of five cell lines for the propagation of bovine viral diarrhea and infectious bovine rhinotracheitis viruses. | a comparative study was carried out to determine the susceptibility of 5 different cell types to bovine viral diarrhea (bvd) and infectious bovine rhinotracheitis (ibr) viruses. the cell systems used were swine testicle (st), mink lung (ml), bovine turbinate (bt), porcine kidney (pk15) and equine dermal (ed) cells. for bvd virus, the titers obtained on day 8 post-infection were 10(1.13), 10(3.25), 10(4.13), 10(0.00) and 10(0.00), in st, ml, bt, pk15 and ed cells, respectively. for the ibr virus, ... | 1987 | 3041184 |
| [evaluation of synthetic activity of lymphocyte nucleoli and phagocytic activity in peripheral blood leukocytes in heifers after infection with infectious bovine rhinotracheitis virus]. | the biosynthetic activity of the nucleoli of peripheral blood lymphocytes in calves, and the proportion of lymphocytes with the micronucleoli, with compact and ring-shaped nucleoli, were evaluated after experimental intranasal and intratracheal infections with the infectious bovine rhinotracheitis (ibr) virus. ibr virus belongs to the group of herpes viruses, the control of which is dominated by cellular immunity. the results are compared with the values for phagocytizing neutrophils, phagocytic ... | 1987 | 3122402 |
| protection of newborn calves against fatal multisystemic infectious bovine rhinotracheitis by feeding colostrum from vaccinated cows. | to determine whether consumption of colostrum with high levels of serum neutralizing antibody to bovine herpesvirus 1 would protect neonatal calves from the frequently fatal multisystemic form of infectious bovine rhinotracheitis, holstein calves were fed for 48 h after birth with either pooled colostrum from seropositive vaccinated cows or colostrum from seronegative unvaccinated cows. the serum neutralizing antibody achieved in the former calves was between 64 and 256 and the titer in the latt ... | 1987 | 2839276 |
| isolation of infectious bovine rhinotracheitis virus from a latently infected feral pig. | infectious bovine rhinotracheitis (ibr) virus was isolated from the trigeminal ganglion of a feral pig after dexamethasone treatment. three pigs inoculated intranasally with the ibr virus did not respond clinically or serologically. the virus was re-isolated from tonsillar swabs from two animals on post-infection day (pid) 3. | 1987 | 2821673 |
| the inhibition of bovine herpesvirus-1 by methyl 2-pyridyl ketone thiosemicarbazone and its effects on bovine cells. | methyl 2-pyridyl ketone thiosemicarbazone (mpkt) was found to inhibit bovine herpesvirus-1 (bhv-1) at an ed50 concentration of approximately 5-10 microm. several virus strains were shown to have similar sensitivity to the drug and serial passage of virus in the presence of mpkt failed to yield resistant progeny. there was evidence for toxic effects on cells at drug concentrations similar to those required to inhibit virus and passage of cells in low concentrations of mpkt gave results suggesting ... | 1987 | 2821894 |
| vaccination of rabbits with a bovine herpesvirus type 1 subunit vaccine: adjuvant effect of iscoms. | bovine herpesvirus type 1 has two major immunogenic surface glycoproteins: a 90 kda haemagglutinin and the 130 (74 + 54) kda glycoprotein. these proteins were purified by rate zonal sucrose density gradient centrifugation after extraction with nonionic detergent triton x-100. for the preparation of iscoms, the glycoproteins were further adsorbed during a second rate zonal centrifugation, to micelles of quil a glycoside, already added to the gradient. haemagglutinating peaks were collected and us ... | 1987 | 2823496 |
| specificity of the indirect enzyme-linked immunosorbent assay for detection of pseudorabies virus antibodies in pigs exposed to bovine herpesvirus-1. | six 5-week-old pigs were inoculated intranasally (in) with 10(7.6) tcid50 of bovine herpesvirus-1 (bhv-1). three of the pigs also were inoculated iv with a similar dose of bhv-1. clinical responses were not observed in these 6 pigs before oronasal challenge exposure with 10(7.8) tcid50 of virulent pseudorabies virus (prv) at postinoculation day 42. two pigs inoculated in with bhv-1 and challenge exposed with prv remained healthy, whereas the remaining 4 pigs developed severe clinical signs of ps ... | 1987 | 2823651 |
| mapping bovine herpesvirus type 1 latency-related rna in trigeminal ganglia of latently infected rabbits. | here we have used the bovine herpesvirus type 1 (bhv-1) rabbit model together with in situ nucleic acid hybridization to identify and map viral rna present in latently infected neurons. radioactively labeled cloned hindiii fragments representing most of the bhv-1 genome (cooper strain) were individually hybridized to sections of trigeminal ganglia taken from rabbits during acute and latent stages of infection. whereas all viral genomic fragments hybridized to lytically infected tissue culture ce ... | 1987 | 2824817 |
| map location of the thymidine kinase gene of bovine herpesvirus 1. | bovine herpesvirus 1 has been reported to contain a thymidine kinase (tk) gene which is nonessential for virus replication. we have isolated a thymidine kinase-negative mutant of the virus and localized the mutation by marker rescue experiments to a 1.1-kilobase bglii-sali fragment which maps at 0.47 to 0.48 on the bovine herpesvirus 1 genomic map. a thymidine kinase-negative bovine cell line isolated in our laboratory was used in these studies. | 1987 | 2824831 |
| bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination. | the 87,000-dalton glycoprotein (gp87) of bovine herpesvirus type 1 (bhv-1) was found to selectively attach to susceptible cells. the attachment of gp87 to the cells was markedly decreased by the prior adsorption of intact virions. anti-gp87 (site ia) monoclonal antibody, which inhibited bhv-1 adsorption to the cells and neutralized the virus without complement [okazaki et al., virology 250: 260-264], was effective in inhibiting the adsorption of gp87. only the same antibody was able to inhibit t ... | 1987 | 2827610 |
| rapid, sensitive, competitive serologic enzyme-linked immunosorbent assay for detecting serum antibodies to bovine herpesvirus type 1. | an enzyme-linked immunosorbent assay (elisa) for the detection of cattle antibodies to bovine herpesvirus type 1 was developed on the basis of competition between serum antibody and a virus-neutralizing mouse monoclonal antibody. the assay showed improved sensitivity over the virus neutralization (vn) test and over an enhanced vn test in which incubation of antibody-virus mixtures was carried out for 24 h. with the elisa, antibodies in sera from experimentally infected cattle were detected earli ... | 1987 | 2828419 |
| [cellular and humoral immune responses in calves after experimental infection with bovine herpesvirus 1]. | studied were some of the humoral and cellular factors of immunity with calves experimentally infected with herpes virus 1. the clinical signs manifested by the infected calves were followed up for 10 to 12 days. the shedding of the virus through the nasal mucosa was demonstrated up to the eighth day, the peak values reached being between the 3rd and 5th day of infection. demonstrated was blastic transformation in vitro with a phytohemagglutinin of the lymphocytes obtained from the peripheral blo ... | 1987 | 2830708 |
| temporal control of bovine herpesvirus 1 glycoprotein synthesis. | the gi, giii, and giv glycoproteins are major bovine herpesvirus 1 antigens involved in virus neutralization. results indicate that the gi and giv glycoproteins were expressed as beta proteins, whereas the giii glycoprotein was expressed strictly as a gamma protein. these findings suggest that gi and giv may be superior to giii as vaccine candidates. | 1987 | 2442412 |
| influence of isoprinosine on bovine herpesvirus type-1 infection in cattle. | a study was conducted to determine the in vivo efficacy of isoprinosine (iso) in calves infected with bovine herpesvirus type-1 (bhv-1). calves were infected with bhv-1 on day 0 and received iso daily for 14 days. clinical signs of disease, shedding of bhv-1, lymphocyte proliferative responses to mitogens, interleukin-2 production, and alveolar macrophage bactericidal activity were monitored during the study. rectal temperatures were increased (p less than 0.05) in bhv-1 and iso-bhv-1 calves at ... | 1987 | 2442882 |
| the in vitro exposure to bovine rhinotracheitis virus of zona pellucida-micromanipulated bovine embryos with the zona pellucida damaged or removed. | one hundred and eighty-five embryos were collected from 29 superovulated donors 6 to 8 d post estrus. the zona pellucida (zp) of these embryos was either cracked, removed mechanically or removed with acidified tyrode's solution, or left intact. forty-eight of 103 (47%) zp-cracked and zp-free embryos, exposed for 24 h to infectious bovine rhinotracheitis virus (ibrv), survived. no significant difference was found in the embryonic survival of the zp-cracked embryos exposed to ibrv and control embr ... | 1987 | 16726332 |
| inactivation of bovine herpesvirus-1 (bhv-i) from in vitro infected bovine semen. | frozen-thawed bovine semen, experimentally infected with bovine herpesvirus-1 (bhv-1) at levels of 10(3) tcid(50)/ml and 10(4) tcid(50)/ml, was treated with a 0.3% trypsin solution to determine the effect of trypsin on the virus and on fertilization using superovulated animals. virus was not isolated from any trypsin-treated samples using a cell culture assay system. nor did two calves develop antibodies to bhv-1 following inoculation with trypsin-treated semen pooled from six bulls. nonsurgical ... | 1988 | 16726507 |
| clinical and pathological evaluation of sulbactam/ampicillin for treatment of experimental bovine pneumonic pasteurellosis. | an experiment was conducted to evaluate the efficacy of sulbactam/ampicillin for treatment of bovine pneumonic pasteurellosis. twenty-one hereford calves were experimentally infected with bovine herpesvirus-1 and an ampicillin-resistant strain of pasteurella haemolytica, then treated for three days with either sulbactam/ampicillin, chloramphenicol, or a placebo. the treatments were evaluated by comparing clinical illness scores, total sick days, weight changes, mortality rates, and postmortem lu ... | 1988 | 17422967 |
| association of bovine viral diarrhea virus with multiple viral infections in bovine respiratory disease outbreaks. | we investigated eleven outbreaks of naturally occurring bovine respiratory diseases in calves and adult animals in the st-hyacinthe area of quebec. specific antibodies to bovine herpesvirus-1, bovine viral diarrhea virus, respiratory syncytial virus, parainfluenza type 3 virus, reovirus type 3, and serotypes 1 to 7 of bovine adenovirus were found in paired sera from diseased animals. several bovine viruses with respiratory tropism were involved concomitantly in herds during an outbreak of bovine ... | 1988 | 17423116 |
| cloned bovine cytolytic t cells recognize bovine herpes virus-1 in a genetically restricted, antigen-specific manner. | the inability to demonstrate bovine herpes virus-1 (bhv-1)-specific lymphocyte responses from bhv-1-infected cattle has been a major difficulty in confirming the importance of cellular effector mechanisms during bhv-1 infection. we have examined the capacity of bovine cytolytic t-cell clones to lyse bhv-1-infected, concanavalin a-stimulated blast cells. cytolysis was as high as 58% at an effector to target (e:t) ratio of 1:1. all cytolytic t-cell clones produced were genetically restricted in ki ... | 1988 | 2448231 |
| monoclonal antibody analysis of bovine herpesvirus-1 glycoprotein antigenic areas relevant to natural infection. | neutralizing antigenic areas on the glycoproteins of bovine herpesvirus-1 (bhv-1) were identified by reciprocal competition radioimmunoassays using monoclonal antibodies. three interrelated and two independent antigenic areas were identified on the 77-kda (k) giv envelope glycoprotein. antigenic analysis of this protein has not been previously described. four interrelated and one independent antigenic areas were found on the 97k giii envelope glycoprotein. a third group of monoclonal antibodies ... | 1988 | 2457277 |
| association of bovine respiratory syncytial virus with atypical interstitial pneumonia in feedlot cattle. | thirty-three cattle with fatal respiratory tract disease were examined for gross and histologic lesions and for the presence of viral and bacterial agents in the lungs. fifteen cattle had lesions characteristic of atypical interstitial pneumonia (aip), and 18 had other respiratory tract diseases, including infectious bovine rhinotracheitis, shipping fever pneumonia, bronchopneumonia, pulmonary abscess, and edema of the trachea. gross necropsy findings in the cattle with aip were uncollapsed and ... | 1988 | 2458690 |
| combined effects of fasting and diet on interferon production and virus replication in calves infected with a vaccine strain of infectious bovine rhinotracheitis virus. | a study was undertaken to investigate the combined effects of fasting and different diets on interferon (ifn) production and virus replication measured in nasal secretions of calves inoculated with a vaccine strain of infectious bovine rhinotracheitis virus. four groups of calves were inoculated intranasally with infectious bovine rhinotracheitis virus. two groups were inoculated 24 hours after onset of a 3-day fast; upon refeeding, 1 group was fed a maintenance diet (m diet) of hay, and the oth ... | 1988 | 2459999 |
| passively administered neutralizing monoclonal antibodies do not protect calves against bovine herpesvirus 1 infection. | monoclonal antibodies specific for defined epitopes on the gi, giii and giv envelope glycoproteins of bhv-1 were used individually or in glycoprotein-monospecific pools for passive immunization of young calves. although serum antibody titres comparable to those found in naturally infected and recovered calves were achieved, passive immunization failed to prevent the growth of bhv-1 in nasal and ocular mucosa and did not decrease the duration of viral shedding. | 1988 | 2461005 |
| epitope specificity and protective efficacy of the bovine immune response to bovine herpesvirus-1 glycoprotein vaccines. | bovine herpesvirus-1 (bhv-1) envelope glycoproteins gi, giii and giv were individually purified on monoclonal antibody affinity columns and injected intradermally into bhv-1 seronegative calves. the calves developed serum neutralizing antibodies that monospecifically precipitated the immunizing glycoprotein from a preparation of 125i-labelled bhv-1 envelope proteins. a competitive radioimmunoassay using the bovine antisera demonstrated that known functional epitopes had been retained in the glyc ... | 1988 | 2461006 |
| functional and topographical analyses of epitopes on bovine herpesvirus type 1 glycoprotein iv. | bovine herpesvirus type 1 (bhv-1) glycoprotein giv was purified by affinity chromatography. purified preparations showed two distinct components of 71 k and 140 k following electrophoresis in sodium dodecyl sulphate polyacrylamide gels. the polypeptides were separated, excised from the gel and used to immunize rabbits; the resulting antisera showed a high degree of cross reactivity indicating that these polypeptides represent monomeric and dimeric forms of the same glycoprotein. purified giv was ... | 1988 | 2463822 |
| characterization of anti-idiotype reagents to bovine herpesvirus-1 monoclonal antibody. | a monoclonal antibody (mab) to a neutralization epitope on the 97-kd glycoprotein of bovine herpesvirus-1 (bhv-1) was used to prepare an anti-idiotypic antibody in rabbits. purified f(ab')2 fragments of the mab were used to immunize the animals and the sera containing the greatest anti-idiotype activity were identified by elisa. after digestion of the immunoglobulins with pepsin and purification by affinity chromatography, anti-idiotype f(ab')2 fragments reacted specifically with the mab in elis ... | 1988 | 2467429 |
| specificity of cleavage in replicative-form dna of bovine herpesvirus 1. | the linear double-stranded dna genome of herpesvirus as it is present in infectious virions needs to be circularized after infection of host cells and before dna replication. replicative-form genomes have to be cleaved into linear unit-length molecules during virion maturation and are most probably the substrate for inversion of the short segment relative to the long segment of the bovine herpesvirus 1 (bhv-1) genome. those regions of the bhv-1 genome which are functionally involved in these pro ... | 1988 | 2831397 |
| serological investigations for some bacterial and viral pathogens in fallow deer (cervus dama) and wild boar (sus scrofa) of the san rossore preserve, tuscany, italy. | sera of 43 fallow deer (cervus dama) of the san rossore preserve (tuscany, italy) were examined for antibodies against eight pathogens; one proved positive for brucella sp., 21 for listeria monocytogenes, 34 for chlamydia psittaci, three for coxiella burnetii, one for infectious bovine rhinotracheitis virus, 11 for parainfluenza-3 virus, 25 for bovine viral diarrhea virus and six for bovine respiratory syncytial virus. no age and sex difference in the positivity rates and titers was evidenced, w ... | 1988 | 2832622 |
| occurrence of bovine herpesvirus-1 dna in nucleosomes and chromatin of bovine herpesvirus-1-infected cells: identification of a virion-associated protein in chromatin of infected cells. | during virus replication a fraction of the intranuclear dna of bovine herpesvirus-1 (bhv-1) was present in the nucleosomal structure of infected eukaryotic cells, and virion proteins were associated with the chromatin of virus infected cells. synthesis of bhv-1 dna in bovine embryonic lung (bel) cells was found to begin four to six hours post-infection (p.i.) and to continue until at least 24 hours p.i. chromatin isolated from infected cell nuclei at ten hours p.i. contained both bhv-1 viral and ... | 1988 | 2835948 |
| serological survey of herpesvirus infections in wild ruminants of france and belgium. | the presence of antibodies against bovine herpesvirus 1 (bhv-1), bovid herpesvirus 6 (bhv-6), herpesvirus of cervidae type 1 (hvc-1), reindeer herpesvirus, bovine herpesvirus 2 (bhv-2) and bovid herpesvirus 4 (bhv-4) was investigated in wild ruminants of france and belgium between 1981 and 1986. there were no animals serologically positive for bhv-4. antibodies against bhv-2 were demonstrated in roe deer (cervus capreolus) (less than 1%) and chamois (rupicapra rupicapra) (1%) in france. animals ... | 1988 | 2836635 |
| evaluation of an antigen-capture elisa for the detection of bovine herpesvirus type 1 shedding from feedlot cattle. | a total of 457 nasal swab specimens from cases of respiratory disease in 2 feed lots were evaluated for the detection of bovine herpesvirus type 1 (bhv-1) by elisa. thirty-three were found to be positive for bhv-1 by the recovery of infectious virus and 21 of these were positive by elisa, yielding a sensitivity of 64%. fifteen other virus isolations were made and included bovine viral diarrhea viruses, rhinoviruses and parainfluenza type 3 viruses; none of these cases were positive with the bhv- ... | 1988 | 2836986 |
| identification of major antigenic proteins of bovine herpesvirus 1 and their correlation with virus neutralizing activity. | the polypeptides of an australian isolate of bovine herpesvirus 1 were analysed by polyacrylamide gel electrophoresis, and western blotting was used to identify those polypeptides which reacted most strongly with sera from infected animals. approximately 20 polypeptides ranging in molecular weight from 11,000 to 240,000 daltons (11-240k) were identified by 35s-methionine labelling of virus and approximately half of these classed as glycoproteins using 14c-mannose and 3h-glucosamine incorporation ... | 1988 | 2836987 |
| prevalence of antibodies to seven viruses in a flock of ewes in minnesota. | blood samples were collected from a flock of healthy ewes at a university of minnesota research station. sera from these blood samples were tested for antibodies against 7 viruses, using 3 tests (eg, virus-neutralization test for bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, bovine adenovirus type 3, and bovine respiratory syncytial virus; hemagglutination inhibition test for parainfluenza virus type 3; and agar-gel immunodiffusion test for lentivirus of ovine progressive ... | 1988 | 2837112 |
| effect of dietary selenium on the primary and secondary immune response in calves challenged with infectious bovine rhinotracheitis virus. | the present study was conducted to determine if a selenium deficiency affects the primary and secondary humoral immune response of calves challenged with infectious bovine rhinotracheitis virus (ibrv) and to evaluate changes in glutathione peroxidase (gsh-px) and creatine phosphokinase activities associated with selenium deficiency and viral stress. treatments included 1) +se (0.2 mg/kg diet) and 2) -se (0.03 mg/kg diet). calves were adapted to their assigned diets during an 84-d depletion perio ... | 1988 | 2828581 |
| bovine herpesvirus 1: rapid diagnosis of infection by direct filter hybridization. | direct filter hybridization (dfh) was applied as a simple method of nucleic acid hybridization to diagnose bovine herpesvirus 1 (bhv-1) infection without previous purification of nucleic acids from the specimens. the dna of bhv-1 was cleaved with the restriction endonuclease pst i and randomly cloned into pkh47 plasmids. the clones were labelled with 32p or biotin and selected on uninfected and infected cells for the highest specific activity to detect bhv-1 infection. two clones, which detected ... | 1988 | 2845258 |
| sequence of a bovine herpesvirus type-1 glycoprotein gene that is homologous to the herpes simplex gene for the glycoprotein gb. | the 130-kda bovine herpesvirus-1 (bhv-1) glycoprotein gvp 6 was found to cross-react immunologically with the herpes simplex glycoprotein gb. antibodies in polyclonal serum against gb immunoprecipitated gvp 6 and its cleavage products from a lysate of bhv-1-infected cells. conversely, polyclonal serum against gvp 6 precipitated gb from hsv-1-infected cell lysates. sera against the other glycoproteins did not demonstrate cross-reactivity. a 3.6-kb kpnl-hpal fragment of bhv-1 dna that hybridized t ... | 1988 | 2845660 |
| propagation and quantitation of animal herpesviruses in eight cell culture systems. | a comparative study was carried out to determine the relative sensitivities of eight different cell culture systems to six different herpesviruses of animals. the cells used were: ofl (ovine fetal lung), ml (mink lung), fk (ferret kidney), ptk-2 (potoroo kidney), tek (turkey embryo kidney), ed (equine dermal), bt (bovine turbinate), and pk15 (porcine kidney). the viruses tested were: prv (pseudorabies) of swine, cphv (caprine herpesvirus), ibrv (infectious bovine rhinotracheitis virus), dn-599 s ... | 1988 | 2846231 |
| effects of a bovine herpesvirus-1 isolate on reproductive function in heifers: classification as a type-2 (infectious pustular vulvovaginitis) virus by restriction endonuclease analysis of viral dna. | a bovine herpesvirus-1 (bhv-1) isolate (fi) from an aborted fetus was used to infect 9 heifers at various stages of gestation. two heifers were inoculated iv on postbreeding day (pbd) 1, 7, or 14, and 3 heifers were inoculated in the sixth month of pregnancy. plasma progesterone assays were used to monitor corpus luteum function in heifers inoculated during early pregnancy. low progesterone values and infertility were seen in the 2 heifers inoculated on pbd 1. luteal function remained normal in ... | 1988 | 2847601 |
| immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with bhv1 and pi3 viruses. | peripheral blood mononuclear cells (pbmc) from calves infected with bovine herpesvirus type 1 (bhv1) or parainfluenza 3 virus (pi3) were cultured in vitro in the presence of inactivated specific antigen presented on mdbk cells. in the presence of inactivated antigen, pbmc from both bhv1-infected and control calves produced interferon (ifn)-alpha in 24 hour cultures. altering the culture conditions did not result in the detection of immune-specific ifn produced by mononuclear cells from bhv1-infe ... | 1988 | 2848301 |
| serological comparisons of antigenically related herpesviruses in cattle, red deer and goats. | serological comparisons were made using related herpesviruses from cattle (bovid herpesvirus 1), red deer (herpesvirus of cervidae 1) and goats (bovid herpesvirus 6) by virus neutralization and enzyme-linked immunosorbent assays. the test samples comprised field sera from british cattle, red deer and goats and sera from experimentally infected or immunized animals. both the cervine and caprine viruses appeared to be more closely related to bovid herpesvirus 1 than they were to each other. cattle ... | 1988 | 2848356 |
| [acid-stable and acid-labile interferons inducible by the bovine infectious rhinotracheitis virus]. | attenuated and virulent strains of cattle infectious rhinotracheitis virus (irtv) induced in cattle leukocytes the production of both acid-stable and acid-labile interferon. the attenuated irtv strain induced the production of the acid-labile interferon in spleen and bone marrow cells of balb/c mice. the indication of the assignment of the acid-labile interferon to the leukocyte-type of interferon consists in the activity of bovine interferon in a heterologous culture of human diploid fibroblast ... | 1988 | 2848365 |
| effects of immunization with bovine herpesvirus-1 glycoproteins on bovine herpesvirus-1-induced alteration of bovine neutrophil chemotactic and anti-pasteurella haemolytica activities. | it has been reported previously that active bovine herpesvirus-1 (bhv-1) infection greatly enhances the susceptibility of cattle to secondary bacterial pneumonia involving pasteurella haemolytica. the present study examines the possibility that immunization of bhv-1 naive calves with purified bhv-1 glycoproteins would protect them against changes in neutrophil function that might compromise their ability to eliminate p. haemolytica during an active bhv-1 infection. the results show that circulat ... | 1988 | 2848379 |
| the effect of substrate on the production of infectious virus by cells in culture. | herpes simplex virus type i (hsv-1), infectious bovine rhinotracheitis virus (ibr) and turkey herpesvirus were examined for growth in cells cultured on three different substrates. the substrates were glass, deae-dextran and collagen gel. with two of the viruses, hsv-1 and ibr, there were no apparent differences in production as a function of substrate. in contrast, the amount of the turkey herpesvirus which was recovered varied greatly with the substrate. titers were highest on glass, followed b ... | 1988 | 2848844 |
| detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity. | a procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. wheat germ lectin, limulus polyphemus lectin and concanavalin a, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. these lectins have also been used for affinity-purification of sets of glycoproteins. the procedure is easy, rapid, cheap and, when biotin is used, non-radioactive. | 1988 | 2848857 |
| [serologic survey of rinderpest, ibr-ipv, rsb, pi3 and bvd-md viruses in cattle in shaba and western zaire]. | 1988 | 2851152 | |
| changing trends in infectious bovine rhinotracheitis in great britain. | the incidence and severity of infectious bovine rhinotracheitis in great britain increased markedly in the mid to late 1970s. data available in maff records have been used to analyse the changing trends in disease incidence and serological prevalence, with particular emphasis on the most recent decade. | 1988 | 2851204 |
| kinetics of large-scale production of bovine leukocyte interferon, using three viral inducers. | kinetics of large-scale production of naturally derived bovine leukocyte interferon (ifn) was investigated using sendai virus, newcastle disease virus, and infectious bovine rhinotracheitis virus inducers. cultures were tested for ifn production every 6 hours for 66 hours. the effect of varying the priming dose of sendai virus from 0 to 50% of total virus dose and the effect of varying the priming time from 0 to 4 hours before induction also were investigated. other factors explored were effects ... | 1988 | 2851950 |
| effect of respiratory infections caused by bovine herpesvirus-1 or parainfluenza-3 virus on bovine alveolar macrophage functions. | calves, 90 to 130 days old, were inoculated with bovine herpesvirus-1 (bhv-1) or parainfluenza-3 (pi-3) virus. pulmonary lavage specimens obtained from calves before virus inoculation contained 98% alveolar macrophages (am) and 1% neutrophils. six days after inoculation, the mean percentage of neutrophils in lavage specimens had significantly increased to 7.9 +/- 6.0% in bhv-1-inoculated calves and to 18.3 +/- 9.9% in pi-3 virus-inoculated calves, reflecting viral-induced pulmonary inflammation ... | 1988 | 2851951 |
| further biological, serological and biochemical characterization of north american, european and southeast asian strains of bovine herpesvirus 1 compared with other alphaherpesvirinae members. | hemagglutination activity, structural protein profiles and neutralization assays were used in a comparative study of bovine herpesvirus 1 strains from the u.s.a., canada, great britain, denmark and malaysia with equine, feline and human herpesviruses in order to further characterize the bovine herpesvirus 1 hemagglutinin. bovine herpesvirus 1 strains of different geographical origins all showed hemagglutinating activity for mouse erythrocytes; furthermore, feline herpesvirus 1 was also shown to ... | 1988 | 2852870 |
| effect of prostaglandins pge2 and pgf alpha 2 on the mean plaque size of bovine herpesvirus 1. | the effect of prostaglandins pge2 and pgf alpha 2 on infectious bovine rhinotracheitis (ibr) virus (bovine herpesvirus 1; bhv-1) was studied by using the measurement of the mean size of plaques produced in cell culture by ibr/cu5 strain. three concentrations (0.1, 1 and 10 micrograms/ml) of prostaglandins, pge2 and pgf alpha 2 were used. each prostaglandin provoked an increase in the mean plaque size when compared to control plaques, at the concentrations 1 and 10 micrograms/ml for pge2 and 10 m ... | 1988 | 2852912 |
| studies on the in vitro biological activities of recombinant bovine tumor necrosis factor (rbotnf) alpha. i. synergistic antiviral efficacy of rbotnf alpha, recombinant bovine interferon gamma (rboifn gamma) and their combination. | the recent demonstration of the antiviral activity of recombinant human tnf has launched an interest in the use of tnf alpha for antiviral therapy in veterinary medicine. in the precent report, we demonstrate that pretreatment of bovine cells with rbotnf alpha reduces the yield of bovine herpesvirus type-1 (bhv-1) from infected cells. reduction in yield was similar to that observed in the presence of rboifn gamma. similarly, rbotnf alpha was able to protect bovine cells from virus-induced cytopa ... | 1988 | 2852921 |
| enhancement of infectious bovine keratoconjunctivitis by modified-live infectious bovine rhinotracheitis virus vaccine. | the effects of a modified-live infectious bovine rhinotracheitis virus vaccine (administered ocularly or intranasally) on experimentally induced infectious bovine keratoconjunctivitis were evaluated. the modified-live infectious bovine rhinotracheitis virus vaccine was administered to 13 male holstein calves (intranasally in 4 and ocularly in 9; day 0). five calves were not vaccinated and served as controls. calves were examined daily and, starting on day 4, moraxella bovis was administered ocul ... | 1988 | 2854704 |
| preincubation of bovine blood neutrophils with bovine herpesvirus-1 does not impair neutrophil interaction with pasteurella haemolytica a1 in vitro. | in this study we examined the direct effects of bovine herpesvirus-1 on the interaction of bovine blood neutrophils with pasteurella haemolytica a1. preincubation of neutrophils for approximately 2 h in vitro with bhv-1 at a multiplicity of infection of 5:1 had no effect on neutrophil random migration and directed migration to zymosan-activated bovine serum. neutrophils also were unimpaired in their ability to ingest and kill p. haemolytica a1. preincubation of neutrophils with bhv-1 did not eli ... | 1988 | 2855289 |
| vaccination to protect calves against infectious bovine rhinotracheitis. | a commercially available inactivated vaccine against infectious bovine rhinotracheitis (bhv1) was tested to assess its ability to immunise young seronegative calves and protect them against challenge with a virulent strain of bhv1. calves showed seroconversion after one or two doses of vaccine. a two-dose and three-dose vaccination regimen each afforded calves significant protection against challenge as judged by the development of clinical symptoms. vaccinated calves were on average 7 to 10 kg ... | 1988 | 2839926 |
| effects of infection with parainfluenza-3 virus and infectious bovine rhinotracheitis virus on neutrophil functions in calves. | calves were challenge exposed in separate experiments with parainfluenza-3 (pi-3) virus or infectious bovine rhinotracheitis (ibr) virus. blood neutrophils were assayed for functional activity every other day for at least 3 weeks by random migration, staphylococcus aureus ingestion, antibody-dependent cell-mediated cytotoxicity, cytochrome-c reduction, iodination, and native chemiluminescence. exposure to pi-3 virus resulted in a brief febrile response and no other clinical signs. alterations in ... | 1988 | 2840008 |
| [surveillance investigations of brucellosis-, leukosis- and bhv-free cattle herds. elisa-based bulk milk studies compared to single animal sample studies with traditional test systems. safety and cost]. | 1988 | 2840257 | |
| characterization of bovine herpesviruses isolated from six sheep and four goats by restriction endonuclease analysis and radioimmunoprecipitation. | viral dna from 10 herpesviruses isolated from 6 sheep and 4 goats were examined by restriction endonuclease analysis with respect to their relatedness to one another; to bovine herpesvirus type 6 (bhv-6), also known as caprine herpesvirus; and to 2 strains of bovine herpesvirus type 1 (bhv-1), known as infectious bovine rhinotracheitis virus (ibrv) and infectious pustular vulvovaginitis virus (ipvv). viral proteins from the isolates were examined by radioimmunoprecipitation with anti-bhv-1/ibrv ... | 1988 | 2840840 |
| comparison of the bovine herpesvirus 1 gi gene and the herpes simplex virus type 1 gb gene. | in a previous report, we localized the gene for a 130-kilodalton envelope glycoprotein (gi) of bovine herpesvirus 1 (bhv-1) to a 3.6-kilobase hpai-kpni restriction endonuclease fragment from the long unique region of the bhv-1 genome (map position 0.405 to 0.432) and showed that a herpes simplex virus 1 (hsv-1) glycoprotein b (gb) probe uniquely hybridized to this bhv-1 restriction fragment. here we present the complete nucleotide sequence of the bhv-1 gi gene and the predicted 932-amino-acid se ... | 1988 | 2841484 |
| associations between viral infection and respiratory disease in young beef bulls. | blood samples were taken from bull calves at two meat and livestock commission performance testing centres, just after weaning at six months of age and at six weekly intervals until the end of the performance test seven months later. sera were assayed by specific elisas for antibodies to bovine herpes virus 1 (bhv1), respiratory syncytial virus, parainfluenza 3 (pi3) and adenoviruses a and b. seroconversions between each sampling were related to the occurrence of clinical respiratory disease usi ... | 1988 | 2842921 |
| an attempt to establish a herd serologically negative for infectious bovine rhinotracheitis. | to improve his position as an exporter of pedigree animals, an owner wished to establish his herd as serologically negative for infectious bovine rhinotracheitis (ibr). after a test on all animals older than six months the herd was split into seronegative and seropositive groups. the seronegative group was tested four times during the first year, twice during the second year and given a final test 12 months later; any positive animals were removed. the results indicated that in the right circums ... | 1988 | 2842923 |
| expression of bovine herpesvirus 1 glycoproteins gi and giii in transfected murine cells. | genes encoding two of the major glycoproteins of bovine herpesvirus 1 (bhv-1), gi and giii, were cloned into the eucaryotic expression vectors prsvcat and psv2neo and transfected into murine lmtk- cells, and cloned cell lines were established. the relative amounts of gi or giii expressed from the two vectors were similar. expression of gi was cell associated and localized predominantly in the perinuclear region, but nuclear and plasma membrane staining was also observed. expression of gi was add ... | 1988 | 2845135 |
| prevalence and specificity of antibodies to bovine respiratory syncytial virus in sera from feedlot and range cattle. | the specificity of serum antibodies for the polypeptides of bovine respiratory syncytial virus (brsv) was examined, using sera obtained from feedlot and range cattle. test results in sera from feedlot cattle indicated a 60% rate of seroconversion and 95% seropositivity to brsv, associated with lack of clinical signs indicative of respiratory tract disease. exposure to other common respiratory tract viruses also was high (greater than or equal to 92% to bovine herpesvirus type 1, bovine viral dia ... | 1988 | 3178027 |
| reproductive difficulties in cattle with antibody titers to haemophilus somnus. | a herd of holstein cows was examined because of suspected embryonic death. four cows had embryonic loss, and 2 cows had aborted. paired serum samples were tested for antibodies to infectious bovine rhinotracheitis virus, bovine viral diarrhea virus, and haemophilus somnus. of 16 cows, 8 had antibody titers to h somnus greater than 1:1,024, and 3 had greater than or equal to four-fold changes in antibody titers to h somnus. haemophilus somnus infection was active in this herd and may have been re ... | 1988 | 3192475 |
| nitrogen kinetics of infectious bovine rhinotracheitis-stressed calves. | eight crossbred feeder steers were used in two consecutive n balance studies to investigate the effects of infectious bovine rhinotracheitis virus (ibrv) on n kinetics. balance study 1, which followed a 10-d acclimation phase, consisted of 7 d of sample collection referred to as the healthy phase (d -7 to d -1). study 2, the ibrv-infected phase, began 2 d after a nasal ibrv challenge and continued for 6 d (d 2 to d 8). a stable isotope, [15n]-glycine, was used to determine n kinetics in both stu ... | 1988 | 3209506 |
| activation of protein breakdown and prostaglandin e2 production in rat skeletal muscle in fever is signaled by a macrophage product distinct from interleukin 1 or other known monokines. | during sepsis or after injection of endotoxin into rats, there is a large increase in muscle protein breakdown and prostaglandin e2 (peg2) production. prior studies showed that partially purified interleukin 1 (il-1) from human monocytes can stimulate these processes when added to isolated rat muscles. the availability of pure recombinant il-1 and other monokines has allowed us to investigate the identity of the active agent in this process. incubation of muscles with recombinant human or murine ... | 1988 | 3284911 |
| de novo initiation of specific cell-mediated immune responsiveness in chickens by transfer factor (specific immunity inducer) obtained from bovine colostrum and milk. | transfer factors (tf) were prepared from colostrum and milk of bovines previously immunized with antigens obtained from coccidioides immitis, infectious bovine rhinotracheitis virus, or from the viral agents responsible for avian newcastle disease, laryngotracheitis disease or infectious bursal disease. the ability of bovine tf to transfer specific cell-mediated immune responsiveness to a markedly xenogenic species was studied using specific pathogen free (spf) and standard commercial (sc) chick ... | 1988 | 2897772 |
| genomic localization and sequence analysis of the putative bovine herpesvirus-1 dna polymerase gene. | the bovine herpesvirus-1 (bhv-1) genome was analysed by southern blot hybridization using the herpes simplex virus type 1 (hsv-1) dna polymerase gene as a probe. a 2.5 kilobase region which hybridized specifically to the hsv-1 dna polymerase gene was identified within the hind iii g fragment at approximate map units 0.334-0.352. in order to provide further evidence that this is the location of the bhv-1 dna polymerase gene, the 2.5 kilobase region was cloned and part of it sequenced. an uninterr ... | 1988 | 2829796 |