Publications

analysis of preexistent vertebral rotation in the normal infantile, juvenile, and adolescent spine.	vertebral rotation was systematically analyzed in the normal, nonscoliotic thoracic spine of children aged 0 to 16 years. subgroups were created to match the infantile, juvenile, and adolescent age groups according to the criteria of the scoliosis research society.	2011	21240053
is neoadjuvant chemotherapy prior to radio-chemotherapy beneficial in t4 anal carcinoma?	this study retrospectively describes the outcome of a series of 38 patients (pts) with t4 anal carcinoma exclusively treated by radio and chemotherapy.	2011	21240983
induction chemotherapy for advanced squamous cell carcinoma of the paranasal sinuses.	to review the oncologic outcomes in patients with advanced (stage iii-iv) squamous cell carcinoma of the paranasal sinuses treated with induction chemotherapy prior to definitive local therapy.	2011	21242552
metformin decreases thyrotropin in overweight women with polycystic ovarian syndrome and hypothyroidism.	to assess the effect of metformin administration on thyroid function in overweight women with polycystic ovarian syndrome (pcos).	2011	21262871
thyroid hormone therapy modulates hypothalamo-pituitary-adrenal axis.	to observe the influence of thyroid hormone therapy on hypothalamo-pituitary-adrenal (hpa) axis, a group of 14 athyreotic women due to thyroid cancer treatment were studied before and after thyroid suppression therapy with thyroxine (t4). changes in plasma adrenocorticotropin (acth) and cortisol levels in response to human corticotropin-releasing hormone (hcrh; 100µg, i.v.) were estimated under hypothyroid conditions and after t4 suppression therapy with 2.5µg/kg/day for two months (n=14). a gro ...	2011	21263198
quantitative analysis of promoter methylation of the ednrb gene in gastric cancer.	hypermethylation has been shown in the promoter region of the endothelin receptor b (ednrb) gene in several human tumors. however, its role in gastric cancer formation is still unclear. in this study, the methylation status of the ednrb gene in paired gastric cancer tissues and adjacent normal tissues from 96 patients was detected quantitatively using pyrosequencing. the results showed the methylation of promoter of ednrb gene in gastric cancer (50.42 ± 9.03%) was significantly higher than in ad ...	2011	21264540
rna and dna bacteriophages as molecular diagnosis controls in clinical virology: a comprehensive study of more than 45,000 routine pcr tests.	real-time pcr techniques are now commonly used for the detection of viral genomes in various human specimens and require for validation both external and internal controls (ecs and ics). in particular, ics added to clinical samples are necessary to monitor the extraction, reverse transcription, and amplification steps in order to detect false-negative results resulting from pcr-inhibition or errors in the technical procedure. here, we performed a large scale evaluation of the use of bacteriophag ...	2011	21347398
risk factors and clinical courses of chemoradiation-related arterio-esophageal fistula in esophageal cancer patients with clinical invasion of the aorta.	although concurrent chemoradiotherapy (crt) is a standard treatment for esophageal cancer invading adjacent structures (t4-ec), arterio-esophageal fistula (aef) occurs occasionally as a critical adverse event of t4-ec with crt. the frequency, clinical course, and risk factors of aef related to crt are not well known.	2011	21347631
[horner syndrome and brachial plexus blockade after epidural anesthesia for obstetric labor and cesarean section].	a 30-year-old woman (asa ii, obese) in her 40th week of a first pregnancy required epidural analgesia for labor. when the cervix had dilated to 5 cm, the epidural infusion was started with a 9-ml bolus of 0.2% ropivacaine and 50 pg of fentanyl, after a negative test dose. an infusion of 0.2% ropivacaine and 1 microg/ml of fentanyl was started at a rate of 8 ml/h. a cesarean section was required after insufficient progress was made during 8 hours of labor. eight milliliters of 0.75% ropivacaine w ...	2011	21348219
predictors of the use of supplemental androgen suppression therapy and external beam radiation in men with high-risk prostate cancer undergoing brachytherapy in community practice.	we assessed clinical- and practice-related variables associated with the use of trimodality treatment (androgen suppression therapy, external beam radiation therapy, and brachytherapy) in a community-based cohort of men with high-risk prostate cancer.	2011	21349778
elevated thyroid-stimulating hormone level in a euthyroid neonate caused by macro thyrotropin-igg complex.	elevated thyroid-stimulating hormone (tsh) was discovered by routine neonatal screening in a newborn with no clinical symptoms. thyroid function tests were repeated and confirmed a high tsh value but normal total thyroxine (t4) and triiodothyronine (t3). however, the mother also had elevated serum tsh with normal levels of t4 and t3. the results suggested a transmitted maternal interfering factor, and no treatment was started while further investigation was performed. gel filtration chromatograp ...	2011	21352360
tumor length as a prognostic factor in esophageal squamous cell carcinoma.	tumor size is an important prognostic factor in many cancers, but its role in esophageal cancer remained undetermined. the aim of this study is to investigate the impact of tumor length on survival for patients with resected esophageal squamous cell carcinoma.	2011	21353021
thyroid-stimulating hormone (tsh): measurement of intracellular, secreted, and circulating hormone in xenopus laevis and xenopus tropicalis.	thyroid-stimulating hormone (tsh) is an important regulator of the hypothalamic-pituitary-thyroid (hpt) axis in xenopus laevis. to evaluate the role of this hormone on developing tadpoles, immunologically-based western blots and sandwich elisas were developed for measuring intracellular (within pituitaries), secreted (ex vivo pituitary culture), and circulating (serum) amounts. despite the small size of the tadpoles, these methods were able to easily measure intracellular and secreted tsh, and c ...	2011	21354158
discrepancies between two alternative staging systems (european neuroendocrine tumor society 2006 and american joint committee on cancer/union for international cancer control 2010) of neuroendocrine neoplasms of the pancreas. a study of 50 cases.	the aim of our study was to identify and describe potential inconsistencies between two alternative staging systems of pancreatic neuroendocrine neoplasms (pnens)--the european neuroendocrine tumor society (enets) system (2006) and the american joint committee on cancer/union for international cancer control (ajcc-uicc) system (2010). to address this issue, we performed a retrospective clinico-pathological study of 50 cases of pnens. we found 9 (18%) cases of enets/ajcc-uicc discrepancies regard ...	2011	21354717
[reference values for thyroid hormones in the population of pregnant women in jaen (spain)].	to evaluate thyroid function in the three trimesters of pregnancy in healthy women taking iodine and to define the reference ranges of normality in this population.	2011	21354872
a promiscuous dna packaging machine from bacteriophage t4.	complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. during genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. the capsid expands after about 10%-25% of the genome is packaged. when the head is full, the motor cuts the concatemeric dna and dissociates from the head. conformational changes, particularly in the portal, are thought to drive these sequential tr ...	2011	21358801
factors influencing the outcome of breast cancer patients with 10 or more metastasized axillary lymph nodes.	background: the purpose of this study was to investigate prognostic factors in breast cancer patients with metastasis of ten or more lymph nodes (pathologic n3a). methods: we conducted a retrospective analysis of the cases of 304 breast cancer patients with pathologic n3a disease who had undergone definitive surgery between 1986 and 2006, and investigated the correlation between clinicopathologic characteristics and treatment outcomes. results: with a median follow-up period of 55 months, the 5- ...	2011	21360123
first trimester maternal serum papp-a and free ß-hcg levels in hyperemesis gravidarum.	to evaluate whether hyperemesis gravidarum (hg) affects first-trimester maternal serum papp-a and free ß-hcg levels.	2011	21360554
effects of propofol anesthesia and sevoflurane anesthesia on the differentiation of human t-helper cells during surgery.	surgical stress causes a helper t-cell type 2 (th2)-dominant status and disturbs the th1/th2 cytokine balance. anesthesia can suppress the stress response to surgery, therefore it may inhibit the imbalance in the th1/th2 ratio. in this study, we assessed if propofol anesthesia and sevoflurane anesthesia influence the th1/th2 cytokine balance, and which anesthesia method better attenuates this ratio.	2011	21362275
effect of intraoperative saline fill volume on perioperative outcomes in tissue expander breast reconstruction.	the effect of tissue expander saline fill volume on perioperative complications in breast reconstruction is unclear. the authors evaluated patients undergoing immediate breast reconstruction with tissue expanders with varying saline fill volumes to determine patient-, surgery-, and disease-related factors associated with complication risk.	2011	21364408
hes1 is required for appropriate morphogenesis and differentiation during mouse thyroid gland development.	notch signalling plays an important role in endocrine development, through its target gene hes1. hes1, a bhlh transcriptional repressor, influences progenitor cell proliferation and differentiation. recently, hes1 was shown to be expressed in the thyroid and regulate expression of the sodium iodide symporter (nis). to investigate the role of hes1 for thyroid development, we studied thyroid morphology and function in mice lacking hes1. during normal mouse thyroid development, hes1 was detected fr ...	2011	21364918
potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in tehran, iran.	this study investigated the occurrence of free-living amoebae (fla) in immunodeficiency wards of hospitals in tehran, iran. a total of 70 dust and biofilm samples from wards serving transplant, pediatric (malignancies), hiv, leukemia and oncology patients of five university hospitals were collected and examined for the presence of fla using culturing and molecular approaches. based on the morphology of the amoebae in plate cultures, primer sets were applied for molecular identification of acanth ...	2011	21365453
perinatal exposure to low-dose de-71 increases serum thyroid hormones and gonadal osteopontin gene expression.	polybrominated diphenyl ethers (pbdes) are flame retardants that have been widely used in manufacturing. they are major household and environmental contaminants that bioaccumulate. humans are exposed primarily through dust inhalation and dietary ingestion of animal products. in animal studies, high doses of penta-brominated diphenyl ethers (penta-bdes) in the mg/kg body weight (bw) range negatively impact brain development, behavior, memory, circulating thyroid hormone concentrations, the reprod ...	2011	21367881
transverse mesocolon invasion in advanced gastric cancer: should we reconsider current t staging?	according to the ajcc/uicc tnm classification, t mesocolon invasion in agc is classified as t2b or t3 according to the presence or the absence of serosa invasion. however, many authors have considered t mesocolon invasion in agc as t4. this study was performed to evaluate the appropriate t stage for t mesocolon invasion in agc.	2011	21369743
differential expression of the a2,3-sialic acid residues in breast cancer is associated with metastatic potential.	aberrant sialylation is closely associated with the malignant phenotype of cancer cells and metastatic potential. however, the precise nature of the molecules in breast cancers has not been unveiled. in this study, we investigated the expression levels of a2,3-sialic acid residues of 50 primary tumor cases, 50 pair-matched lymph node metastasis tumor samples and in the mda-mb-231, t-47d and mcf-7 breast cancer cell lines with different metastatic potential. the expression of a2,3-sialic acid res ...	2011	21344161
total thyroidectomy: a safe and effective treatment for graves' disease.	thyroidectomy as a first line treatment for graves' disease is rarely utilized in the us. the purpose of this study was to analyze the safety and efficacy of thyroid surgery among patients with graves' disease.	2011	21345453
[robotic-assisted laparoscopic radical cystectomy: evaluation of functional and oncological results].	radical cystectomy remains the most effective treatment for patients with localized, invasive bladder cancer and recurrent noninvasive disease. we report our experience with 84 consecutive cases of robotic assisted laparoscopic radical cystectomy with regard to perioperative results, pathological outcomes and surgical complications.	2011	21345519
effect of subzero-balanced ultrafiltration on lung gas exchange capacity after cardiopulmonary bypass in adult patients with heart valve disease.	this study was conducted to evaluate the effect of a new ultrafiltration technique--the subzero-balanced ultrafiltration (sbuf)--on lung gas exchange capacity after cardiopulmonary bypass (cpb) in adult patients with heart valve disease.	2011	21345773
correlation between iodine intake and thyroid function in subjects with normal thyroid function.	excessive iodine intake is known to induce hypothyroidism in people who have underlying thyroid disorders. however, few studies have been performed on subjects with normal thyroid function without a history of autoimmune thyroid disease. we hypothesized that high iodine intake may cause a subtle change in thyroid function even in subjects with normal thyroid function. we analyzed 337 subjects (64 men and 273 women; mean age, 49 years) who showed normal levels of thyroid peroxidase antibodies (tp ...	2011	21340678
localized tongue amyloidosis in a patient with neurofibromatosis type ii.	localized amyloidosis (al) may rarely involve oral mucosa. this is the first known reported case describing the development of tongue al in a 30-year-old patient with neurofibromatosis (nf) type-2.	2011	21340708
prognostic significance of adverse events associated with preoperative radiotherapy for rectal cancer.	adverse events may occur in patients receiving preoperative radiotherapy (prt) for rectal cancers. the aim of this study is to clarify the clinical and pathological features of the patients with prt-related adverse events, and the significance of the adverse events on the clinical outcome.	2011	21340716
oncological outcomes in patients undergoing radical nephrectomy and vena cava thrombectomy for renal cell carcinoma with venous extension: a single-centre experience.	to report on the effectiveness of the surgical management of renal cell carcinoma (rcc) in patients with a neoplastic thrombus of the vena cava.	2011	21330093
a randomized phase ii study of 5-fluorouracil, hydroxyurea, and twice-daily radiotherapy compared with bevacizumab plus 5-fluorouracil, hydroxyurea, and twice-daily radiotherapy for intermediate-stage and t4n0-1 head and neck cancers.	introduction: we conducted a randomized phase ii study to evaluate the impact of adding bevacizumab (b) to 5-fluorouracil (5-fu), hydroxyurea (hu), and radiotherapy (fhx) for intermediate-stage and select t4 head and neck squamous cell cancers (hnscc). patients and methods: eligible patients had newly diagnosed hnscc. randomization was 2:1 in favor of bfhx. all patients received 500 mg hu p.o. b.i.d., 600 mg/m(2)/day continuous infusion 5-fu, and b.i.d. radiotherapy with or without bevacizumab 1 ...	2011	21330337
intravitreal recombinant tissue plasminogen activator without and with additional gas injection in patients with submacular haemorrhage associated with age-related macular degeneration.	purpose: to compare intravitreal recombinant tissue plasminogen activator (rt-pa) treatment with and without gas injection in patients with submacular haemorrhage associated with age-related macular degeneration. methods: we conducted a retrospective, non-randomized comparative case study of 110 eyes from 76 women and 34 men (mean age 78.1 ± 6.9 years). evaluations of the data were performed for baseline visit (t1), and visits 1-3 weeks (t2), 3 months (t3) and 6 months after (t4). the patients ...	2011	21332673
thoracoscopic resection of symptomatic herniated thoracic discs: clinical results in 121 patients.	abstract: study design: retrospective review of a prospectively maintained surgical database.objective: report the indications for, surgical procedures performed, and outcomes of the largest series of thoracoscopically treated herniated thoracic discs (htds). we also compared approach-related complications to an unmatched cohort undergoing thoracotomy for htd.summary of background data: symptomatic htds are rare and their surgical management is technically challenging.methods: a prospectively ma ...	2011	21336237
inactivation of escherichia coli and bacteriophage t4 by high levels of dissolved co2.	little information is available regarding the effectiveness of water disinfection by co(2) at low pressure. the aim of this study was to evaluate the use of high levels of dissolved co(2) at 0.3-0.6 mpa for the inactivation of microorganisms. bacteriophage t4 was chosen as the model virus and escherichia coli was selected as the representative bacterium. the results of the study showed a highly effective log inactivation of e. coli and bacteriophage t4 at low and medium initial concentrations by ...	2011	21336924
mycobacterium tuberculosis expresses ftse gene through multiple transcripts.	bacterial ftse gene codes for the atp-binding protein, ftse, which in complex with the transmembrane protein, ftsx, participates in diverse cellular processes. therefore, regulated expression of ftse and ftsx might be critical to the human pathogen, mycobacterium tuberculosis, under stress conditions. although ftsx gene of m. tuberculosis (mtftsx) is known to be transcribed from a promoter inside the upstream gene, ftse, the transcriptional status of ftse gene of m. tuberculosis (mtftse) remains ...	2011	21336990
influence of organic selenium supplementation on the accumulation of toxic and essential trace elements involved in the antioxidant system of chicken.	the aim of the study was to investigate the interactions between selenium (se) and various trace elements, both toxic and essential, involved in the antioxidant system. a total of 128 day-old chicks (gallus gallus, broilers) were used to investigate the effect of se yeast supplementation on the accumulation of cadmium (cd), copper (cu) iron (fe) and zinc (zn). there were four replicates of four dietary treatments: t1 (basal diet with no added se, analyzed to contain 0.21 mg kg(-1)), t2 (t1 with ...	2011	21337231
intra-operative radiotherapy of rectal cancer: results of the french multi-institutional randomized study.	to assess efficacy and tolerance of intra-operative radiation therapy (iort) in patients suffering from locally advanced rectal cancer, treated with preoperative radiotherapy followed by surgical resection.	2011	21339010
human papillomavirus-related squamous cell carcinoma of the oropharynx: a comparative study in whites and african americans.	to evaluate the frequency of human papillomavirus-related oropharyngeal squamous cell carcinoma in african americans and whites and to examine patient outcomes in these 2 groups.	2011	21339403
syringomyelia associated with syphilitic spinal meningitis: real complication or possible association?	the study has been designed as a case report.	2011	21339760
lack of action of exogenously administered t3 on the fetal rat brain despite expression of the monocarboxylate transporter 8.	mutations of the monocarboxylate transporter 8 gene (mct8, slc16a2) cause the allan-herndon-dudley syndrome, an x-linked syndrome of severe intellectual deficit and neurological impairment. mct8 transports thyroid hormones (t4 and t3), and the allan-herndon-dudley syndrome is likely caused by lack of t3 transport to neurons during critical periods of fetal brain development. to evaluate the role of mct8 in thyroid hormone action in the fetal brain we administered t4 or t3 to thyroidectomized pre ...	2011	21303950
skeletal identification by radiographic comparison: blind tests of a morphoscopic method using antemortem chest radiographs.	this study investigated the value of antemortem (am) and postmortem (pm) radiographs of the claviculae and c3-t4 vertebrae to identify skeletons of missing u.s. soldiers from past military operations. in total, 12 field-recovered skeletons and am chest radiographs of 1460 individuals were used. for each skeleton, examiners analyzed an array of am chest radiographs (up to 1000 individuals) and attempted to identify the correct pm/am radiographic match. when examiners were able to compare all imag ...	2011	21306373
immunomarker studies of fine-needle cytopuncture cell blocks for tumor response prediction after preoperative chemotherapy and prognosis in operable nonmetastatic primary breast carcinoma.	neo-adjuvant chemotherapy of breast cancer provides an opportunity to evaluate predictive factors at initial tumor biopsy. we evaluated these factors on cell blocks obtained by diagnostic fine-needle cytopuncture (fnc), with respect to tumor regression and outcome. a prospective study (1996-2003, median follow-up 82 months) involved 163 patients with breast carcinoma (t2 = 3 cm, t3, t4 noninflammatory) diagnosed by means of fnc. malignancy, cytologic grade, and the presence of lymphocytes were d ...	2011	21306468
right pneumonectomy for t4 non-small cell lung cancer invading intrapericardial pulmonary vein in a patient with a right aortic arch.	a 59 year-old man with a right-sided aortic arch who had a t4 right lung cancer invading the proximal superior pulmonary vein underwent an intrapericardial-pneumonectomy with partial left atrium resection and a radical lymphadenectomy. the presence of a right-sided aortic arch required particular attention during dissection of the lymph nodes. this is the first case of a right-pneumonectomy for t4-lung cancer in a patient with a right-sided aortic arch.	2011	21306950
pott disease in a 13-month-old: case report.	tuberculosis (tb) is a common disease worldwide that is caused by mycobacterium tuberculosis. tb of the spine is the most common site of bony infection and is often referred to as pott disease. to the best of our knowledge, our case represents the youngest patient with naturally acquired pott disease in the united states.	2011	21307787
high sensitivity 5-hydroxymethylcytosine detection in balb/c brain tissue.	dna hydroxymethylation is a long known modification of dna, but has recently become a focus in epigenetic research. mammalian dna is enzymatically modified at the 5(th) carbon position of cytosine (c) residues to 5-mc, predominately in the context of cpg dinucleotides. 5-mc is amenable to enzymatic oxidation to 5-hmc by the tet family of enzymes, which are believed to be involved in development and disease. currently, the biological role of 5-hmc is not fully understood, but is generating a lot ...	2011	21307836
external validation of the proposed t and n categories of squamous cell carcinoma of the penis.	objectives: the aim of this study was to validate recently proposed modifications to the current tnm classification of penile squamous cell carcinoma (pscc) by using data from four german urological centers. methods: we identified 89 patients treated for histologically confirmed pscc between 1996 and 2008 and reclassified them according to the proposed tnm staging revisions. the proposed changes restricted t2 to tumoral invasion of the corpus spongiosum, whereas invasion of the corpus cavernos ...	2011	21309862
comparison of the prognostic accuracy of the sixth and seventh editions of the tnm classification for intrahepatic cholangiocarcinoma.	the seventh tnm edition introduced a new, specific staging structure for intrahepatic cholangiocarcinoma (ihc).	2011	21309938
prediction of protein-protein interactions between human host and a pathogen and its application to three pathogenic bacteria.	molecular understanding of disease processes can be accelerated if all interactions between the host and pathogen are known. the unavailability of experimental methods for large-scale detection of interactions across host and pathogen organisms hinders this process. here we apply a simple method to predict protein-protein interactions across a host and pathogen organisms. we use homology detection approaches against the protein-protein interaction databases, dip and ipfam in order to predict int ...	2011	21310175
lipid profile, thyroid function, and serum magnesium level in type i diabetic children.	this study aim was to investigate the changes of lipid profile, thyroid function and magnesium (mg) level in type i diabetic children.	2011	21311427
effects of methylphenidate on performance of a practical pistol shooting task: a quantitative electroencephalography (qeeg) study.		2011	21294887
two-year results from a swedish study on conventional versus accelerated radiotherapy in head and neck squamous cell carcinoma - the artscan study.	studies on accelerated fractionation (af) in head and neck cancer have shown increased local control and survival compared with conventional fractionation (cf), while others have been non-conclusive. in 1998 a national swedish group decided to perform a randomised controlled clinical study of af.	2011	21295880
a rare case of anterior thoracic myelomeningocele with scoliosis--case report and review of the literature.	spinal deformities associated with spinal dysraphism are a challenging problem. myelomeningocele has been reported in the lumbar spine. however, it is rare in the thoracic spine.	2011	21296287
recombinant human thyroid stimulating hormone in thyroid remnant ablation with 1.1 gbq 131iodine in low-risk patients.	objective: to evaluate the efficacy of recombinant human thyroid stimulating hormone [rhtsh (versus hypothyroidism)] in thyroid ablation with an activity of 1.1 gbq (30 mci) i. methods: a total of 102 patients with thyroid cancer who fulfilled the following criteria were studied: submitted to total thyroidectomy with complete tumor resection; tumor =4 cm without extrathyroid invasion or lymph node metastases; negative anti-thyroglobulin (anti-tg) antibodies. thirty-two patients (group a) receive ...	2011	21297429
discovering conformational sub-states relevant to protein function.	internal motions enable proteins to explore a range of conformations, even in the vicinity of native state. the role of conformational fluctuations in the designated function of a protein is widely debated. emerging evidence suggests that sub-groups within the range of conformations (or sub-states) contain properties that may be functionally relevant. however, low populations in these sub-states and the transient nature of conformational transitions between these sub-states present significant c ...	2011	21297978
clinicopathologic features and prognosis analysis of mucinous gastric carcinoma.	mucious gastric carcinoma (mgc) is a subtype of gastric carcinoma and its clinicopathologic features and prognosis still remain unclear. to investigate the clinical significance and surgical outcomes of mucinous gastric carcinoma, 2,769 patients with gastric carcinoma were analyzed in a case control study. we reviewed the records of 196 patients with mucinous gastric carcinoma and 2,573 with nonmucinous gastric carcinoma (ngc). clinicopathologic features and survival rate of patients were analyz ...	2011	21298368
enhancer of zeste homolog 2 overexpression in nasopharyngeal carcinoma: an independent poor prognosticator that enhances cell growth.	purpose: as a key component of polycomb-repressive complex 2, enhancer of zeste homolog 2 (ezh2) represses target genes through histone methylation and is frequently overexpressed and associated with poor prognosis in common carcinomas. for the first time, we reported ezh2 expression and its biological and clinical significance in nasopharyngeal carcinoma (npc). methods and materials: in npc cell lines and specimens, endogenous expression of ezh2 mrna and protein was determined by semiquantitati ...	2011	21300475
a novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic dna.	recently, 5-hydroxymethylcytosine (5hmc) was identified in mammalian genomic dna. the biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in elucidating its function. we describe a method for the rapid and inexpensive identification of genomic regions containing 5hmc. this method involves the selective glucosylation of 5hmc residues by the ß-glucosyltransferase from t4 bacteriophage creating ß-glucosyl-5-hydroxymethylc ...	2011	21300643
ossification of the midpalatal suture after surgically assisted rapid maxillary expansion.	the purpose of this study was to evaluate ossification of the midpalatal suture in adult patients immediately after surgically assisted rapid maxillary expansion (sarme) until 120 days post-surgery. the sample comprised 126 standardized occlusal radiographs of 21 adults (14 females and 7 males; mean age: 25.33 years) taken pre-expansion (t1), immediately after expansion (t2), and post-expansion (30, 60, 90, and 120 days of retention, respectively: t3, t4, t5, and t6) who had undergone sarme. the ...	2011	21300726
clinical evaluation of the function of hypothalamo-pituitary-thyroid axis in children with central nervous system infections.	it is well known that certain non-thyroidal critical illness may lead to euthyroid sick syndrome(ess). there are little reports about the change of thyroid hormone in the children's central nervous system (cns) infections.	2011	21314949
marginal iodide deficiency and thyroid function: dose-response analysis for quantitative pharmacokinetic modeling.	severe iodine deficiency (id) results in adverse health outcomes and remains a benchmark for understanding the effects of developmental hypothyroidism. the implications of marginal id, however, remain less well known. the current study examined the relationship between graded levels of id in rats and serum thyroid hormones, thyroid iodine content, and urinary iodide excretion. the goals of this study were to provide parametric and dose-response information for development of a quantitative model ...	2011	21315791
features of time-intensity curve parameters of colorectal adenocarcinomas evaluated by double-contrast enhanced ultrasonography: initial observation.	purpose: this study is to investigate the value of double contrast-enhanced ultrasonography (dceu) in assessing microcirculation of colorectal adenocarcinomas and to describe the perfusion features of the tumours. material and methods: dceus was performed in 42 patients with adenocarcinoma. the time-intensity curve parameters (arrival time (at), time-to-peak (ttp), peak intensity (pi) and area under the curve (auc)) within the tumours were extracted. the parameters were compared among the tumour ...	2011	21316889
t4 or mi?		2011	21317744
association of the ugt1a1-53(ta)n polymorphism with l-thyroxine doses required for thyrotropin suppression in patients with differentiated thyroid cancer.	there is a considerable interindividual variation in l-thyroxine [3,5,3',5'-tetraiodo-l-thyronine (t4)] dose required for thyrotropin (thyroid-stimulating hormone) suppression in patients with differentiated thyroid cancer. to investigate whether uridine diphosphate-glucuronosyl transferase 1a1 (ugt1a1)-mediated t4 glucuronidation in liver affects t4 dose, we genotyped 101 patients for the common ugt1a1-53(ta)n polymorphism and compared t4 doses among patients having zero (5/6 and 6/6 genotypes) ...	2011	21317830
concurrent cisplatin, continuous infusion fluorouracil and radiotherapy followed by tailored consolidation treatment in non metastatic anal squamous cell carcinoma.	to evaluate efficacy and feasibility of chemo-radiotherapy in patients with non-metastatic anal squamous-cell-cancer.	2011	21291546
cdna library generation from ribonucleoprotein particles.	most, if not all, known noncoding rnas (ncrnas) are associated with rna binding proteins, thus forming ribonucleoprotein particles or rnps. here we describe a protocol for the generation of a specialized cdna library from rnps, thereby increasing the proportion of functional ncrna species in the library. to that end, cellular extracts are fractionated on 10-30% glycerol gradients. subsequently, rnp-derived ncrnas are isolated and 3'-tailed by cytidine triphosphate and poly(a) polymerase; this is ...	2011	21293458
fluorescence amplified fragment length polymorphism for subtyping of genotypes of acanthamoeba isolated from patients with keratitis.	acanthamoeba keratitis (ak) is a painful and vision-threatening ocular infection. the differentiation of acanthamoeba at the species and subspecies level is complicated. nearly all the ak isolates have been shown to belong to t4 genotype when analysed by ribosomal rna gene sequences and there is no universally acceptable method for differentiation of different subtypes of t4. the purpose of this study was to attempt further discrimination of t4 genotypes.	2011	21321424
persistence of goitre in the post-iodization phase: micronutrient deficiency or thyroid autoimmunity?	despite years of salt iodization, goitre continues to be a major public health problem worldwide. we examined the prevalence of goitre in the post-iodization phase and the relationship of goitre with micronutrient status and thyroid autoimmunity in school children of chandigarh, north india.	2011	21321427
megalin and androgen receptor gene expression in young and old human skeletal muscle before and after three sequential exercise bouts.	androgen signaling occurs primarily via the androgen receptor. megalin, a low-density lipoprotein endocytic receptor located in various mammalian tissues, has been recently shown to facilitate sex hormone–binding globulin (shbg) steroid complexes across cell membranes. the purpose of this investigation is to determine if the megalin gene is expressed in human skeletal muscle and if present to determine how megalin and androgen receptor mrna expression change in response to sequential exercise bo ...	2011	21322835
type 2 iodothyronine deiodinase is expressed in human preadipocytes.	type 2 iodothyronine deiodinase (d2) is an enzyme that catalyzes the production of triiodothyronine (t3) from thyroxine (t4) and plays a critical role in providing the local intracellular t3. although d2 is highly expressed in brown adipose tissue, it was thought that d2 is hardly expressed in white adipose tissue. in the present study, we examined whether d2 is expressed in human preadipocytes, using human mesenteric and subcutaneous preadipocytes (hmpa and hscpa, respectively).	2011	21323585
type of skin eruption is an independent prognostic indicator for adult t-cell leukemia/lymphoma.	cutaneous involvement is seen in ~ 50% of adult t-cell leukemia/lymphoma (atll) patients. we investigated the association between skin eruption type and prognosis in 119 atll patients. atll eruptions were categorized into patch (6.7%), plaque (26.9%), multipapular (19.3%), nodulotumoral (38.7%), erythrodermic (4.2%), and purpuric (4.2%) types. when the t stage of the tumor-node-metastasis-blood (tnmb) classification of mycosis fungoides/sézary syndrome was applied to atll staging, 16.0% were t1, ...	2011	21325600
myogenic mrna markers in young and old human skeletal muscle prior to and following sequential exercise bouts.	this study examined how multiple bouts of conventional resistance training affected the mrna expression of transcripts and a protein associated with satellite cell activity in human skeletal muscle. ten younger men (means ± se; age, 21.0 ± 0.5 years; body mass, 82.3 ± 4.2 kg; height, 178.4 ± 2.2 cm; percent body fat, 15.4% ± 2.9%) and 10 older men (age, 66.4 ± 1.6 years; body mass, 94.2 ± 3.7 kg; height, 180.9 ± 2.2 cm; percent body fat, 27.4% ± 1.8%) completed 3 lower-body workouts (monday, wed ...	2011	21326383
the incidence of thyroid dysfunction following radiotherapy for early stage carcinoma of the larynx.	external beam radiotherapy is currently the commonest form of treatment for early laryngeal cancer (t1/2) and thyroid dysfunction is a well recognised complication of this treatment. overt hypothyroidism is a specific clinical disorder which in most patients will be recognized and treated. the problems associated with subclinical hypothyroidism (elevated tsh with normal t4), however, are only now being recognized and their management is to some extent still controversial. the aims of our study a ...	2011	21328003
a comparison of different initialization protocols to obtain statistically independent molecular dynamics simulations.	we study how the results of molecular dynamics (md) simulations are affected by various choices during the setup, e.g., the starting velocities, the solvation, the location of protons, the conformation of his, asn, and gln residues, the protonation and titration of his residues, and the treatment of alternative conformations. we estimate the binding affinity of ligands to four proteins calculated with the mm/gbsa method (molecular mechanics combined with a generalized born and surface area solva ...	2011	21132839
husbands' and wives' alcohol use disorders and marital interactions as longitudinal predictors of marital adjustment.	in this longitudinal study, the relationships among wives' and husbands' lifetime alcoholism status, marital behaviors, and marital adjustment were tested. participants were 105 couples from the michigan longitudinal study (mls), an ongoing multimethod investigation of substance use in a community-based sample of alcoholics, nonalcoholics, and their families. at baseline (t1), husbands and wives completed a series of diagnostic measures, and lifetime diagnosis of alcohol use disorder (aud, as de ...	2011	21133510
thyroid storm in a patient with fulminant hepatic failure.	this manuscript describes a 28-year-old patient with a history of graves' disease who was transferred to tulane university hospital with fulminant hepatic failure. he reported associated nausea, vomiting, anorexia, as well as jaundice and abdominal discomfort for a period of 3 weeks prior to his admission. his thyroid function tests on admission were tsh, 0.013 μu/ml; t3, 94.9 μu/ml; t4, 9.37 μu/ml; free t4, >6 μu/ml. his liver function tests were characteristic of hepatic failure. the patient u ...	2011	21120833
local response to chemoradiation in t4 larynx cancer with cartilage invasion.	patients with cartilage invasion were excluded from organ preservation protocols treating laryngeal and hypopharyngeal cancer. treatment choice between chemoradiotherapy (crt) and total laryngectomy (tl) remains controversial for these patients.	2011	21120838
uncalibrated arterial pulse cardiac output measurements in patients with moderately abnormal left ventricular function.	the aim of the study was to evaluate the accuracy and precision of the vigileo/flotrac system (edwards lifesciences, irvine, ca) when compared with the intermittent cardiac output and continuous cardiac output measurements obtained from pulmonary arterial catheters in patients with moderately abnormal left ventricular function undergoing elective coronary artery bypass graft surgery.	2011	20850989
lymph node ratio provides prognostic information in addition to american joint committee on cancer n stage in patients with melanoma, even if quality of surgery is standardized.	to investigate whether lymph node ratio (lnr) gives additional prognostic information to american joint committee on cancer (ajcc) n stage in a melanoma treatment center where regional lymph node dissection (rlnd) techniques are standardized.	2011	21119509
accuracy of free-hand pedicle screws in the thoracic and lumbar spine: analysis of 6816 consecutive screws.	pedicle screws are used to stabilize all 3 columns of the spine, but can be technically demanding to place. although intraoperative fluoroscopy and stereotactic-guided techniques slightly increase placement accuracy, they are also associated with increased radiation exposure to patient and surgeon as well as increased operative time.	2011	21150762
long-term aspirin use and the risk of total, high-grade, regionally advanced and lethal prostate cancer in a prospective cohort of health professionals, 1988-2006.	experimental studies suggest a role for aspirin in the chemoprevention of prostate cancer and epidemiological evidence supports a modest inverse association between regular aspirin use and prostate cancer risk, especially for advanced disease. in a prospective cohort study of 51,529 health professionals aged 40-75 years at baseline, we evaluated long-term aspirin use and the incidence of total, high-grade (gleason 8-10, n = 488), regionally advanced (t3b-t4 or n1, n = 228) and lethal prostate ca ...	2011	21128233
bacterial secretion chaperones.	many gram-negative bacteria are able to invade hosts by translocation of effectors directly into target cells in processes usually mediated by two very complex secretion systems (sss), named type iii (t3) and type iv (t4) sss. these syringe-needle injection devices work with intervention of specialized secretion chaperones that, unlike traditional molecular chaperones, do not assist in protein folding and are not energized by atp. controversy still surrounds secretion chaperones primary role, bu ...	2011	21121896
utility of the novel bladder preservation therapy, boai-cddp-radiation (omc-regimen), for elderly patients with invasive bladder cancer.	in this study, we investigated the novel bladder preservation therapy, the balloon-occluded arterial infusion (boai) of cisplatin/gemcitabine, concomitantly with hemodialysis, along with concurrent irradiation [the 'osaka medical college (omc)-regimen'] in patients >70 years of age with muscle-invasive bladder cancer. eighty-three such patients were assigned to receive either the omc-regimen (n=56) or cystectomy (n=27). the omc-regimen patients who failed to achieve complete response (cr) underw ...	2011	21109921
isolation and characterization of acanthamoeba spp. from air-conditioners in kuala lumpur, malaysia.	during a study on the quality of the indoor environment, acanthamoeba spp. were detected in 20 out of 87 dust samples collected from air-conditioners installed in a four-story campus building located in kuala lumpur, malaysia. twenty-one cloned acanthamoeba isolates designated as imu1 to imu21 were established from the positive primary cultures. five species were identified from the 16 isolates according to the morphological criteria of pussard and pons; i.e. a. castellanii, a. culbertsoni, a. g ...	2011	20858455
overstaging of cartilage invasion by multidetector ct scan for laryngeal cancer and its potential effect on the use of organ preservation with chemoradiation.	the two currently acceptable treatment options for locally advanced laryngeal cancer are total laryngectomy and organ preservation using chemoradiation. to facilitate therapeutic decision making, the accurate pre-treatment evaluation of cartilage invasion is of paramount importance. the purpose of this study was to evaluate the positive predictive value (ppv) and negative predictive value (npv) of detecting neoplastic cartilage invasion in laryngeal cancer patients using fast-speed multidetector ...	2011	20858661
injections of clostridium botulinum neurotoxin a may cause thyroid complications in predisposed persons based on molecular mimicry with thyroid autoantigens.	a woman with hashimoto's thyroiditis, under replacement l-t4, repeatedly experienced, over a 10-year period, elevations of serum tsh after eyelid injections of clostridium botulinum neurotoxin a (btx). we hypothesized a link between btx injections and tsh elevations via molecular mimicry, and aimed to verify our hypothesis. using an in silico approach, we searched first for amino acid sequence homology between btx and thyroid autoantigens, and next for hla binding motifs within homologous segmen ...	2011	21061092
laparoscopic vs open resection for patients with rectal cancer: comparison of perioperative outcomes and long-term survival.	the aim of the study is to assess the safety and oncologic feasibility of laparoscopic-assisted resection for rectal cancer vs open rectal resection as a phase ii pilot study for a planned randomized control trial.	2011	21160307
the burden of prevalent vertebral fractures is negatively associated with functional outcome in women with hip fracture.	the aim of this study was to investigate the association between the burden of prevalent vertebral fractures and functional outcome in women with hip fracture.	2011	21169744
effect of intravenous lidocaine associated with amitriptyline on pain relief and plasma serotonin, norepinephrine, and dopamine concentrations in fibromyalgia.	the objective of this study was to evaluate the effect of intravenous lidocaine combined with amitriptyline on pain relief and plasma serotonin, norepinephrine, and dopamine levels.	2011	21178598
effect of low levels of aflatoxin b₁ on performance, biochemical parameters, and aflatoxin b₁ in broiler liver tissues in the presence of monensin and sodium bentonite.	aflatoxins (af) are a major problem in broiler production and are significant economic and public health burdens worldwide. a commercial sodium bentonite (na-b) adsorbent was used to prevent the effect of af [50 µg of aflatoxin b₁ (afb₁)/kg of feed] in broiler productivity, biochemical parameters, macroscopic and microscopic liver changes, and afb₁ liver residues. the influence of na-b (0.3%) and monensin (mon, 100 mg/kg), alone or in combination, was investigated in depth. the dietary treatment ...	2011	21177443
perfluorooctane sulfonic acid exposure increases cadmium toxicity in early life stage of zebrafish, danio rerio.	exposure to perfluorooctane sulfonic acid (pfos) is known to induce thyroid-related adverse effects in aquatic organisms. because an antioxidant defense mechanism is one of the key functions of the thyroid gland, we examined whether preexposure to pfos could disrupt thyroid function and enhance cadmium (cd)-induced oxidative stress in fish. zebrafish embryos were exposed to control or 0.5 mg/l pfos for 7 d after fertilization and subsequently exposed to 0.038 mg/l of cd(2+) or a mixture of the p ...	2011	21184528
predicting thyroxine requirements following total thyroidectomy.	optimal thyroxine replacement following total thyroidectomy is critical to avoid symptoms of hypothyroidism. the aim of this study was to determine the best formula to determine the initiated replacement dose of levothyroxine immediately following total thyroidectomy.	2011	21198738
developmental trajectories of marijuana use from adolescence to adulthood: personal predictors.	to investigate the relationship between early adolescent personal characteristics and the developmental trajectories of marijuana use extending from early adolescence to adulthood.	2011	21199981
thymidylate synthase genotype-directed neoadjuvant chemoradiation for patients with rectal adenocarcinoma.	downstaging (ds) of rectal cancers is achieved in approximately 45% of patients with neoadjuvant fluorouracil (fu) -based chemoradiotherapy (crt). polymorphisms in the thymidylate synthase gene (tyms) had previously defined two risk groups associated with disparate tumor ds rates (60% v 22%). we conducted a prospective single-institution phase ii study using tyms genotyping to direct neoadjuvant crt for patients with rectal cancer.	2011	21205745
high-pressure epr reveals conformational equilibria and volumetric properties of spin-labeled proteins.	identifying equilibrium conformational exchange and characterizing conformational substates is essential for elucidating mechanisms of function in proteins. site-directed spin labeling has previously been employed to detect conformational changes triggered by some event, but verifying conformational exchange at equilibrium is more challenging. conformational exchange (microsecond-millisecond) is slow on the epr time scale, and this proves to be an advantage in directly revealing the presence of ...	2011	21205903
dual modification of a triple-stranded β-helix nanotube with ru and re metal complexes to promote photocatalytic reduction of co2.	we have constructed a robust β-helical nanotube from the component proteins of bacteriophage t4 and modified this nanotube with ru(ii)(bpy)(3) and re(i)(bpy)(co)(3)cl complexes. the photocatalytic system arranged on the tube catalyzes the reduction of co(2) with higher reactivity than that of the mixture of the monomeric forms.	2011	21206940
single-stranded dna binding proteins unwind the newly synthesized double-stranded dna of model miniforks.	single-stranded dna binding (ssb) proteins are essential proteins of dna metabolism. we characterized the binding of the bacteriophage t4 ssb, escherichia coli ssb, human replication protein a (hrpa), and human hssb1 proteins onto model miniforks and double-stranded-single-stranded (ds-ss) junctions exposing 3' or 5' ssdna overhangs. t4 ssb proteins, e. coli ssb proteins, and hrpa have a different binding preference for the ss tail exposed on model miniforks and ds-ss junctions. the t4 ssb prote ...	2011	21189045
serum triiodothyronine level as an indicator of inflammation in patients undergoing dialysis.	introduction. it has been shown that inflammation affects thyroid function. in patients with end-stage renal disease, low plasma triiodothyronine (t3) may be an unsuspected expression of the inflammatory state of these patients. this study evaluated the correlation between t3 and high-sensitivity c-reactive protein (hscrp) levels in patients on peritoneal dialysis (pd) and hemodialysis. materials and methods. this is a cross-sectional study aiming at the correlation between t3 and hscrp levels a ...	2011	21189433
association of paraoxonase 1 (pon1) polymorphisms with osteoporotic fracture risk in postmenopausal korean women.	there is increasing evidence of a biochemical link between lipid oxidation and bone metabolism. paraoxonase 1 (pon1) prevents the oxidation of low-density lipoprotein (ldl) and metabolizes biologically active phospholipids in oxidized ldls. here, we performed association analyses of genetic variation in pon1 to ascertain its contribution to osteoporotic fractures (ofs) and bone mineral density (bmd). we directly sequenced the pon1 gene in 24 korean individuals and identified 26 sequence variants ...	2011	21187701