Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| structure of bacteriophage t4 endonuclease ii mutant e118a, a tetrameric giy-yig enzyme. | coliphage t4 endonuclease ii (endoii), encoded by gene dena, is a small (16 kda, 136 aa) enzyme belonging to the giy-yig family of endonucleases, which lacks a c-terminal domain corresponding to that providing most of the binding energy in the structurally characterized giy-yig endonucleases, i-tevi and uvrc. in vivo, it is involved in degradation of host dna, permitting scavenging of host-derived nucleotides for phage dna synthesis. endoii primarily catalyzes single-stranded nicking of dna; 5- ... | 2010 | 20156453 |
| late occurrence of cervicothoracic ossification of posterior longitudinal ligaments in a surgically treated thoracic opll patient. | ossification of the posterior longitudinal ligament (opll) in the thoracic spine is rare, even in the far east. a 45-year-old female presented with a 4-month history of progressive motor weakness in the lower extremities, numbness below the midthoracic area, and spastic gait disturbance. neuroradiological examinations revealed massive oplls at the t4-t6 levels with severe anterior compression of the spinal cord. anterior decompressive corpectomies with bone grafts were performed from t4 to t6 us ... | 2010 | 20157380 |
| modeling the electrostatic signature of single enzyme activity. | charge sensors based on nanoscale field-effect transistors are a promising new tool to probe the dynamics of individual enzymes. however, it is currently unknown whether the electrostatic signals associated with biological activity exceed detection limits. we report calculations of electrostatic signatures of two representative enzymes, deoxyribonuclease i and t4 lysozyme. our simulations reveal that substrate binding to deoxyribonuclease and internal dynamics of lysozyme are detectable at the s ... | 2010 | 20163162 |
| management of sinonasal inverted papillomas and comparison of classification staging systems. | the aim of this study was to describe treatment results in patients with sinonasal inverted papilloma and to compare recent classification staging systems. | 2010 | 20109330 |
| potential to use ultraviolet-treated bacteriophages to control foodborne pathogens. | the use of replication-deficient uv-treated bacteriophages, or phages, presents an alternative to viable phages for food biocontrol applications. nontransducing uv-treated phages, if used correctly, are unlikely to produce viable progeny phages, which might otherwise mediate undesirable horizontal gene transfer events. phage t4 and escherichia coli were used as a model system to examine this possibility. uv-treated phages were able to cause a reduction in the optical density of outer membrane-fr ... | 2010 | 20113208 |
| predictors of occult nodal metastasis in colon cancer: results from a prospective multicenter trial. | the relationship between primary colon cancer and occult nodal metastases (oms) detected by cytokeratin immunohistochemistry (ck-ihc) is unknown. we sought to investigate the correlation of clinicopathologic features of colon cancer with oms and to identify predictors of om. | 2010 | 20116081 |
| preoperative therapy with trastuzumab and oral vinorelbine (+/- endocrine therapy) in patients with her2-positive breast cancer. | combined trastuzumab and intravenous vinorelbine yielded high clinical activity as preoperative treatment in patients (pts) with her 2/neu positive breast cancer. | 2010 | 20117001 |
| eeg activity during the verbal-cognitive stage of motor skill acquisition. | this study examined changes in eeg activity associated with motor performance during the verbal-cognitive stage of skill learning. participants (n=14) were required to practice a sequential finger tapping task. eeg activity was recorded both before and after short-term practice, during finger tapping and during two control conditions. eeg power (fz, cz, pz, t3, t4) and coherence (t3-fz, t4-fz, fz-cz, fz-pz) were computed for the theta (4-8 hz), slow alpha (8-10 hz), fast alpha (10-12 hz), slow b ... | 2010 | 20117168 |
| is the mechanisms of fipronil-induced thyroid disruption specific of the rat: re-evaluation of fipronil thyroid toxicity in sheep? | in rat, fipronil treatment increases the elimination of thyroid hormones (th). relevance of this mechanism for the risk of fipronil for human health is subject to controversy because of the specificities of adult rat for th plasma binding properties which often lead to the assumption that rats are more sensitive than human to thyroid disruption. this study aimed at determining if the mechanism of fipronil-induced thyroid disruption is altered in sheep a species more relevant to human from the st ... | 2010 | 20117195 |
| [changes of thyroxin and monocyte human leukocyte antigen-dr expression in senior patients with sepsis]. | o investigate the changes of thyroxin and monocyte human leukocyte antigen-dr expression in senior patients with sepsis and explore their clinical significance. | 2010 | 20118006 |
| effects of epidural fentanyl on speed and quality of block for emergency cesarean section in extending continuous epidural labor analgesia using ropivacaine and fentanyl. | we performed a prospective, randomized, and double-blind study comparing the top-up effects of 2% lidocaine/100 microg fentanyl/epinephrine (n=31) and 2% lidocaine/saline/epinephrine (n=30) when extending an epidural labor analgesia using low-dose ropivacaine and fentanyl. survival analysis for the sensory blocks to the t4 level showed no statistically significant differences in onset time to t4 between the 2 groups. onset times (min) to t4-sensory blocks for cold and pinprick were not different ... | 2010 | 20119585 |
| the use of sugammadex in a patient with myasthenia gravis. | myasthenia gravis, affecting neuromuscular transmission, leads to a large variability in sensitivity to depolarising and non-depolarising neuromuscular blocking drugs. we report the successful use of the modified mu-cyclodextrin sugammadex in a myasthenic patient to reverse a rocuronium-induced deep level of neuromuscular block. after spontaneous neuromuscular recovery of t2 (second twitch of the train-of-four series), we administered 2 mg.kg(-1) of sugammadex intravenously, reversing neuromuscu ... | 2010 | 20105151 |
| hypopituitarism is uncommon after aneurysmal subarachnoid haemorrhage. | aneurysmal subarachnoid haemorrhage (sah) has recently been reported as a common cause of chronic hypopituitarism, and introduction of routine neuroendocrine screening has been advocated. we aimed at estimating the risk of hypopituitarism after sah using strict criteria including confirmatory testing in case of suggested insufficiency. | 2010 | 20105184 |
| childhood minimally invasive follicular carcinoma: clinical features and immunohistochemistry analysis. | to report on two cases of childhood thyroid minimally invasive follicular carcinoma (mifc) to highlight the clinical features, laboratory findings and diagnosis of this rare disease. | 2010 | 20105250 |
| complications after sphincter-saving resection in rectal cancer patients according to whether chemoradiotherapy is performed before or after surgery. | the aim of the present study was to compare the influence of preoperative chemoradiotherapy (crt) with postoperative crt on the incidence and types of postoperative complications in rectal cancer patients who underwent sphincter-saving resection. | 2010 | 20106604 |
| [paraplegia after prolonged cardiac arrest and extracorporeal life support]. | paraplegia by medullar ischaemia after cardiac arrest is rarely reported, especially without an oxo-ischaemic cerebral lesions. we report the case of a 17-year-old woman who underwent a 70 minute cardiac arrest with recovery of consciousness, but a symptomatic medullar ischaemia (paraplegia under t4). | 2010 | 20106627 |
| reduced stratifin expression can serve as an independent prognostic factor for poor survival in patients with esophageal squamous cell carcinoma. | stratifin plays an important role in cancer biology by interfering with intracellular signalling pathways and cell-cycle checkpoints. decreased expression of stratifin gene has been reported to be a poor prognostic indicator in a variety of human malignant tumors. | 2010 | 20108042 |
| the star chart to ta bladder cancer: an unsophisticated analysis of two-dimensional gel electrophoresis proteome maps. | to explore the use of two-dimensional gel electrophoresis (2de) for analysing the proteome of clinically relevant tissue samples such as biopsies from transurethral resections of the bladder (turb), by generating a ta proteome map, possibly identifying technical or biological artefacts, and searching for biological subgroups associated with clinical data. | 2010 | 20059407 |
| influence of class m1 glutathione s-transferase (gst mu) polymorphism on gst m1 gene expression level and tumor size in oral squamous cell carcinoma. | glutathione s-transferases (gst) are antioxidant enzymes and oxidative stress markers in oral carcinogenesis. they present a system of polymorphic proteins. some variants are associated with increased sensitivity to toxic compounds, as it is known for the gstm1-null variant allele. however, the influence of the gstm1 allele variant genotype on gstm1-mrna quantity in oral squamous cell carcinoma (oscc) and normal mucosa as well as the impact on prognosis remains unclear. the genotype for gstm1 (m ... | 2010 | 20060357 |
| novel multifunctional pharmaceutical excipients derived from microcrystalline cellulose-starch microparticulate composites prepared by compatibilized reactive polymer blending. | the choice of excipients remains a critical factor in pharmaceutical formulations. microcrystalline cellulose-maize starch composites (mcc-mst) have been prepared by mixing colloidal dispersions of microcrystalline cellulose (mcc) with 10% (w/w) of chemically gelatinized maize starch (mst) at controlled temperature conditions for use as multifunctional excipients with direct compression and enhanced disintegration abilities. the novel excipient was evaluated for its direct compression and enhanc ... | 2010 | 20060448 |
| dna crunching by a viral packaging motor: compression of a procapsid-portal stalled y-dna substrate. | many large double-stranded dna viruses employ high force-generating atp-driven molecular motors to package to high density their genomes into empty procapsids. bacteriophage t4 dna translocation is driven by a two-component motor consisting of the procapsid portal docked with a packaging terminase-atpase. fluorescence resonance energy transfer and fluorescence correlation spectroscopic (fret-fcs) studies of a branched (y-junction) dna substrate with a procapsid-anchoring leader segment and a sin ... | 2010 | 20060554 |
| parental bonding and self-esteem as predictors of severe depressive symptoms: a 10-year follow-up study of norwegian physicians. | elevated rates of suicide and depression among physicians have been reported. the associations between perceived parental bonding and depressive symptoms have yet to be studied longitudinally in this occupational group. in a nationwide cohort, we sought to study parental bonding as a predictor for severe depressive symptoms and to determine whether self-esteem mediates this relationship. after graduation (t1), medical students (n = 631) were followed-up after 1 (t2), 4 (t3), and 10 (t4) years. t ... | 2010 | 20061865 |
| outcomes after stereotactic lung radiotherapy or wedge resection for stage i non-small-cell lung cancer. | purpose to compare outcomes between lung stereotactic radiotherapy (sbrt) and wedge resection for stage i non-small-cell lung cancer (nsclc). patients and methods one hundred twenty-four patients with t1-2n0 nsclc underwent wedge resection (n = 69) or image-guided lung sbrt (n = 58) from february 2003 through august 2008. all were ineligible for anatomic lobectomy; of those receiving sbrt, 95% were medically inoperable, with 5% refusing surgery. mean forced expiratory volume in 1 second and diff ... | 2010 | 20065181 |
| thyroid hormone prevents cognitive deficit in a mouse model of alzheimer's disease. | this study aimed to examine the feasibility of using thyroid hormone (th) as a therapeutic agent for alzheimer's disease (ad). mice were injected intra-hippocampally aggregated amyloid beta-peptide (abeta) to produce ad animal model. intraperitoneal administration of l-thyroxine (l-t4) into abeta-induced ad model mice prevented their cognitive impairment and improved their memory function. the mechanisms of l-t4 treating ad might be associated with regulating cholinergic function, protecting the ... | 2010 | 20045708 |
| the enzymatic activity of type 1 iodothyronine deiodinase (d1) is low in liver hemangioma: a preliminary study. | type 1 iodothyronine deiodinase (d1) is a crucial enzyme which converts the prohormone thyroxine (t4) into active tri-iodothyronine (t3). there has been strong evidence that the metabolism of thyroid hormones is disturbed in some neoplastic tissues such as thyroid, renal, and breast cancer. however, there are few available data about d1 enzyme activity in benign tumors such as hemangioma, which is the most common primary liver tumor. hence this study aimed to determine the enzymatic activity of ... | 2010 | 20049650 |
| short-term in-home intervention reduces depressive symptoms in early head start latina mothers of infants and toddlers. | depressive symptoms may compromise the ability of low-income latina mothers with limited english language proficiency to parent their infants or toddlers. eighty early head start latina mothers with limited english language proficiency were randomized to an advanced practice nurse-delivered, culturally tailored, in-home psychotherapy intervention, or to usual care. repeated measures regression analysis showed a significantly greater decrease in depressive symptoms for intervention mothers compar ... | 2010 | 20043296 |
| development of tefs for pcb congeners by using an alternative biomarker--thyroid hormone levels. | polychlorinated biphenyls (pcbs) are ubiquitous toxic contaminants. health risk assessment for this class of chemicals is complex: the current toxic equivalency factor (tef) method covers dioxin-like (dl-) pcbs, dibenzofurans, and dioxins, but excludes non-dl-pcbs. to address this deficiency, we evaluated published data for several pcb congeners to determine common biomarkers of effect. we found that the most sensitive biomarkers for dl-non-ortho-pcb 77 and pcb 126 are liver enzyme (e.g., ethoxy ... | 2010 | 20043972 |
| is elective irradiation to the lower neck necessary for n0 nasopharyngeal carcinoma? | to summarize our experience and treatment results in lymph node-negative nasopharyngeal carcinoma treated in a single institution. | 2010 | 20044215 |
| [prophylactic irradiation of cervical lymph nodes for stage-n0 nasopharyngeal carcinoma]. | it is controversial for the irradiation level and dose of the regional prevention for naspharyngeal cancer (npc) with one or both cervical lymph node-negative neck. the study was to analyze the proophylactic irradiation of cervical lymph nodes for stage -n0 npc patients. | 2010 | 20038321 |
| using the rate of respiration to monitor events in the infection of escherichia coli cultures by bacteriophage t4. | the growing interest in applications of bacteriophages creates a need for improvements in the production processes. continuous monitoring of the phage production is an essential aspect of any control strategy and, at present, there is no completely satisfactory option. the approach presented here uses ir-spectrometry to continuously measure the rate of respiration (co(2) released) of escherichia coli infected by phage t4 at various multiplicities of infection (moi). within the trends in these da ... | 2010 | 20039436 |
| optimal placement of a superior vena cava cannula in minimally invasive robot-assisted cardiac surgery. | minimally invasive robot-assisted cardiac surgery is generally performed nowadays. to avoid the interference of a superior vena cava (svc) cannula during surgery, it should be inserted before the operation. the position of this cannula is very important because it can cause poor venous drainage during operation. the proper position of the svc cannula was investigated in the present study. | 2010 | 20032562 |
| lipid profiles in the untreated patients with hashimoto thyroiditis and the effects of thyroxine treatment on subclinical hypothyroidism with hashimoto thyroiditis. | to evaluate the prevalence of dyslipidemia in the population of hashimoto thyroiditis, we reviewed medical records on the consecutive 1181 cases with adult hashimoto thyroiditis and 830 cases were adopted for the study. first, the serum tsh level increased and serum free t4 level decreased, slightly but significantly, with increasing age. there were significant positive correlations between serum tsh levels and lipid parameters such as total cholesterol (tc), triglyceride (tg), hdl-cholesterol ( ... | 2010 | 20032565 |
| optimum lymphadenectomy for esophageal cancer. | using worldwide esophageal cancer collaboration data, we sought to (1) characterize the relationship between survival and extent of lymphadenectomy, and (2) from this, define optimum lymphadenectomy. | 2010 | 20032718 |
| concurrent chemoradiation followed by adjuvant chemotherapy in patients with locoregionally advanced nasopharyngeal carcinoma in korea. | concomitant approach using cisplatin and 5-fluorouracil (5-fu) has shown an excellent local control rate and significantly reduced distant metastasis in patients with locally advanced nasopharyngeal carcinoma (npc). however, optimal schedule and dosing of chemotherapy still need to be developed to reduce distant metastasis. this retrospective study was conducted to evaluate the efficacy, toxicity, and tolerability of a concurrent chemoradiation therapy (ccrt) regimen using cisplatin and 5-fu fol ... | 2010 | 20033411 |
| radiation therapy for feline cutaneous squamous cell carcinoma using a hypofractionated protocol. | the objective of this paper was to evaluate the efficacy of a hypofractionated radiation protocol for feline facial squamous cell carcinoma (scc). twenty-five histologically confirmed sccs in 15 cats were treated with four fractions of 7.6-10gy each, with 1 week intervals. the equipment used was a linear accelerator clinac 2100 delivering electron beam of 4 or 6mev, and a bolus of 5 or 10mm was used in all lesions. of the lesions, 44% were staged as t4, 16% as t3, 8% as t2 and 32% as t1. of the ... | 2010 | 20034827 |
| comparison of video-assisted thyroidectomy and traditional thyroidectomy for the treatment of papillary thyroid carcinoma. | there is concern regarding the oncological effectiveness of minimally invasive video-assisted thyroidectomy (vat) for thyroid carcinoma. this study compared the surgical results of vat and traditional thyroidectomy in patients with small papillary thyroid carcinomas (ptc). | 2010 | 20035348 |
| video. endoscopic minimally invasive thyroidectomy: first clinical experience. | since theodor kocher reduced the mortality rate of thyroidectomy from the 40% reported by billroth to 0.2% in 1895, a collar incision with open removal of the thyroid gland is the standard procedure. in the past decade, efforts were made to reduce incision size and surgical access trauma by the use of endoscopic techniques. a first attempt was replacement of the central "kocher incision" with lateral neck incisions and endoscopic removal of a thyroid lobe by hüscher on 8 july 1996. this lateral ... | 2010 | 20035350 |
| 3d postural balance with regard to gravity line: an evaluation in the transversal plane on 93 patients and 23 asymptomatic volunteers. | relevance of posture assessment has been reported in case of spine disorders. this study explores the interest in quantifying posture using 3d reconstruction from biplanar x-rays in free standing position and a force plate. 93 patients consulting for spine disorders were divided ('3d deformity', 'sagittal imbalance' and 'mild deformity') and compared with 23 asymptomatic volunteers. registration of the gravity line (gl) in reconstruction yielded transversal position of the center of acoustic mea ... | 2010 | 20035359 |
| mesotrypsin promotes malignant growth of breast cancer cells through shedding of cd109. | serine proteases have been implicated in many stages of cancer development, facilitating tumor cell growth, invasion, and metastasis, and naturally occurring serine protease inhibitors have shown promise as potential anticancer therapeutics. optimal design of inhibitors as potential therapeutics requires the identification of the specific serine proteases involved in disease progression and the functional targets responsible for the tumor-promoting properties. here, we use the hmt-3522 breast ca ... | 2010 | 20035377 |
| genetic variability of vascular endothelial growth factor and prognosis of head and neck cancer in a brazilian population. | vascular endothelial growth factor (vegf) is one of the most potent endothelial cell mitogens and plays a critical role in angiogenesis. polymorphisms in this gene have been evaluated in patients with several types of cancer. the objectives of this study were to determine if there was an association of the -1154g/a polymorphism of the vegf gene with head and neck cancer and the interaction of this polymorphism with lifestyle and demographic factors. additionally, the distribution of the vegf gen ... | 2010 | 20098841 |
| [care continuity for patients with congenital hypothyroidism during transition from childhood to adulthood]. | since the nation-wide neonatal screening programs for congenital hypothyroidism(ch) had been implemented in 1979, the diagnosis and treatment of ch are now provided in the first 2-3 weeks of birth in japan. the goals of treatment are to raise the serum t4 as rapidly as possible into the normal range, and maintain normal growth and development while avoiding overtreatment. because transient hypothyroidism may occur frequently, a reevaluation after 3 years is needed in such patients. patients with ... | 2010 | 20077804 |
| sympathetic nervous system effects in the hands following a grade iii postero-anterior rotatory mobilisation technique applied to t4: a randomised, placebo-controlled trial. | joint mobilisation to the t4 vertebra has been advocated as a treatment for t4 syndrome. to date no controlled studies have investigated the effects of thoracic spinal manual therapy (smt) applied to t4 on sympathetic activity in the hands. this study investigated whether a grade iii postero-anterior rotatory joint mobilisation technique applied to the t4 vertebra at a frequency of 0.5 hz had demonstrably greater effects than a validated placebo intervention on skin conductance (sc) in the hands ... | 2010 | 20093065 |
| oxidative stress: a required condition for thyroid cell proliferation. | goiter is associated with increased oxidative stress (os). we studied the effects of an anti-inflammatory agent, 15 deoxy-delta12,14-prostaglandin j2 (15dpgj2) and an antioxidant, n-acetylcysteine (nac), on os, thyroid function, and goiter expansion in a model of goiter induced by propylthiouracil (ptu) or perchlorate. os was assessed by the immunodetection of 4-hydroxynonenal, thyroid function by measuring thyroxin (t4) and thyrotropin (tsh) plasma levels and detecting t4-rich thyroglobulin (tg ... | 2010 | 20093493 |
| lessons from the lysozyme of phage t4. | an overview is presented of some of the major insights that have come from studies of the structure, stability, and folding of t4 phage lysozyme. a major purpose of this review is to provide the reader with a complete tabulation of all of the variants that have been characterized, including melting temperatures, crystallographic data, protein data bank access codes, and references to the original literature. the greatest increase in melting temperature (t(m)) for any point mutant is 5.1 degrees ... | 2010 | 20095051 |
| compensatory mechanisms to maintain blood pressure in paraplegic rats: implication of central tachykinin nk-1 and nk-3 receptors? | people with high level spinal cord injury (sci) suffer from both hypotension and spontaneous hypertension due to loss of supraspinal control of spinal sympathetic outflow. few reports have addressed whether any changes occur in central regulation of blood pressure (bp) and heart rat (hr) at the supraspinal level. central tachykinin nk-1 and nk-3 receptors are located in many cardiovascular areas in the brain and are known to modulate bp and hr. this study examined the intracerebroventricular (i. ... | 2010 | 20096457 |
| metabolic effects of 20-oh-ecdysone in ovariectomized rats. | postmenopausal women develop often obesity which may be prevented by 20-oh-ecdysone (ecd). this was investigated in ovariectomized (ovx) rats. they were orally treated with 3 doses of ecd (18, 56 or 116 mg/day/animal). positive controls received 159 microg estradiol (e2). quantitative computer tomography at the level of the abdomen and the metaphysis of the tibia allowed estimation of surface, fat depots and muscles. the highest dose of ecd resulted in serum concentrations of 0.4 x 10(-6)m. seru ... | 2010 | 20097286 |
| comparison of the molecular influences of no-induced lesions in dna strands on the reactivity of polynucleotide kinases, dna ligases and dna polymerases. | nitric oxide (no) causes dna damage, generating xanthine (xan, x) and oxanine (oxa, o) from guanine (gua, g) and hypoxanthine (hyp, h) from adenine (ade, a) by nitrosative oxidation. although these no-induced lesions have been thought to cause mutagenic problems in cellular systems, the influence of these lesions on enzymatic functions has not yet been compared systematically. in this study, we investigated the effect of no-induced lesions on the activities of dna-binding/recognizing enzymes suc ... | 2010 | 20097903 |
| effect of melatonin supplementation on plasma vasopressin response to different conditions in rats with hyperthyroidism induced by l-thyroxine. | the present study was performed to determine how basal, isotonic, hypertonic and hypovolemic conditions affect fluid-electrolyte balance and plasma arginine vasopressin (avp) levels in rats with experimental hyperthyroidism supplemented with melatonin. the rats were divided into four groups of twenty-four subjects each kept under the following treatments during one month: (1) controls; (2) treated with l-thyroxine; (3) treated with l-thyroxine and sham melatonin and (4) treated with l-thyroxine ... | 2010 | 20079381 |
| characterizing the in vitro hepatic biotransformation of the flame retardant bde 99 by common carp. | polybrominated diphenyl ethers (pbdes) are a class of flame retardant chemicals known to biomagnify in aquatic foodwebs. however, significant biotransformation of some congeners via reductive dehalogenation has been observed during in vivo and in vitro laboratory exposures, particularly in fish models. little information is available on the enzyme systems responsible for catalyzing this metabolic pathway in fish. this study was undertaken to characterize the biotransformation of one primary bde ... | 2010 | 20080306 |
| micron-scale holes terminate the phage infection cycle. | holins are small phage-encoded proteins that accumulate harmlessly in the cytoplasmic membrane during the infection cycle until suddenly, at an allele-specific time, triggering to form lethal lesions, or "holes." in the phages lambda and t4, the holes have been shown to be large enough to allow release of prefolded active endolysin from the cytoplasm, which results in destruction of the cell wall, followed by lysis within seconds. here, the holes caused by s105, the lambda-holin, have been captu ... | 2010 | 20080651 |
| should direct mesocolon invasion be included in t4 for the staging of gastric cancer? | one of the sites most frequently invaded by gastric cancer is the mesocolon; however, the uicc does not mention this anatomical site as an adjacent structure involved in gastric cancer. the purpose of this study was to characterize and classify mesocolon invasion from gastric cancer. | 2010 | 20082356 |
| evaluation of isotope-coded protein labeling (icpl) in the quantitative analysis of complex proteomes. | an evaluation of the icpl (isotope-coded protein labeling) non-isobaric labeling technique was performed using two different biological models. two samples containing phage t4 capsids were mixed in a 1:1 ratio after being labeled with the light or heavy versions of the icpl reagent. the analysis of this proteome demonstrated the feasibility of this approach for differential quantitative proteomics and was employed to optimize the experimental parameters of the icpl workflow. icpl-mediated analys ... | 2010 | 20082807 |
| genetics and phenomics of selenoenzymes--how to identify an impaired biosynthesis? | expression of selenoenzymes relies on the se supply and specific cis- and trans-acting factors. two inherited defects are caused by dysfunctional selenoproteins or insufficient biosynthesis, i.e., selenoprotein n-related myopathy and impaired selenoprotein translation secondary to hypomorphic mutations in sbp2. the latter affects biosynthesis of many selenoenzymes including the deiodinase activities. a strategy to identify individuals carrying a mutation in a specific selenoprotein gene can be b ... | 2010 | 20083162 |
| association between a high number of isolated lymph nodes in t1 to t4 n0m0 colorectal cancer and the microsatellite instability phenotype. | stage i or ii colorectal carcinomas with microsatellite instability (msi) are characterized by more isolated lymph nodes in the resected specimen than their counterparts with microsatellite stability (mss). | 2010 | 20083749 |
| thyroid profile of term appropriate for gestational age neonates in nigeria: a forerunner to screening for congenital hypothyroidism. | thyroid hormones in normal quantity are vital for physical growth and mental development during fetal and postnatal life. the available publications in sub-saharan africa on the thyroid function of newborn infants was reported in babies delivered in moderate to severe goitre zones; utilizing the hormone levels from these studies may have a measure of bias. newborn screening for congenital hypothyroidism (ch) in sub-saharan africa is still a mirage, the technicality of the best timing for collect ... | 2010 | 20085966 |
| systemic thyroid hormone is necessary and sufficient to induce ultraviolet-sensitive cone loss in the juvenile rainbow trout retina. | rainbow trout possess ultraviolet-sensitive (uvs) cones in their retina that degenerate naturally during development. this phenomenon can be induced with exogenous thyroxine [t4, a thyroid hormone (th)] treatment. however, the previous t4 exposure experiments employed static water immersion; a method that could introduce confounding stress effects on the fish. because of this, it was uncertain if t4 alone was sufficient to induce retinal changes or if stress-related hormones were also necessary ... | 2010 | 20086135 |
| advances in the relationship between lymph node status and prognosis: comment on "association between a high number of isolated lymph nodes in t1 to t4 n0m0 colorectal cancer and the microsatellite instability". | 2010 | 20088097 | |
| reaction mechanisms of the multicopper oxidase cueo from escherichia coli support its functional role as a cuprous oxidase. | cueo from escherichia coli is a multicopper oxidase (mco) involved in copper tolerance under aerobic conditions. it features four copper atoms that act as electron transfer (t1) and dioxygen reduction (t2, t3; trinuclear) sites. in addition, it displays a methionine-rich insert which includes a helix that blocks physical access to the t1 site and which provides an extra labile site t4 adjacent to the t1 center. this t4 site is required for cueo function. like many mcos, cueo exhibits phenol oxid ... | 2010 | 20088522 |
| thyroid autoimmunity and treatment response to escitalopram in major depression. | there is evidence that immune alterations play an important part in the pathogenesis of major depression. thyroid autoimmunity has been found in association with major depression in several studies. | 2010 | 20088751 |
| analytic review: thyrotoxic periodic paralysis: a review. | thyrotoxic periodic paralysis (tpp) is an unusual complication of hyperthyroidism that frequently presents in a dramatic fashion, necessitating treatment in an emergency department or admission to an intensive care unit. thyrotoxic periodic paralysis is characterized by transient, recurrent episodes of flaccid muscle paralysis affecting proximal more severely than distal muscles. thyrotoxic periodic paralysis is most commonly a complication of graves' disease in asian males, although in recent d ... | 2010 | 20089526 |
| high-frequency phage-mediated gene transfer in freshwater environments determined at single-cell level. | lateral gene transfer by phages has contributed significantly to the genetic diversity of bacteria. to accurately determine the frequency and range of phage-mediated gene transfer, it is important to understand the movement of dna among microbes. using an in situ dna amplification technique (cycling primed in situ amplification-fluorescent in situ hybridization; cprins-fish), we examined the propensity for phage-mediated gene transfer in freshwater environments at the single-cell level. phage p1 ... | 2010 | 20090786 |
| painless thyroiditis complicated by acromegaly. | the serum thyroid stimulating hormone (tsh) level is decreased in acromegalic patients. although this phenomenon is thought to be caused by the enhanced secretion of somatostatin which suppresses tsh production, it has not yet been proven. we describe a 60-year-old woman with acromegaly who showed a low concentration of tsh. we diagnosed her as painless thyroiditis based on an increased level of thyroglobulin, depressed radioactive iodine uptake (raiu), normal vascularity and mild swelling of th ... | 2010 | 20075583 |
| loss of udp-galnac:polypeptide n-acetylgalactosaminyltransferase 3 and reduced o-glycosylation in colon carcinoma cells selected for hepatic metastasis. | o-glycosylation of mucin is initiated by the attachment of n-acetyl-d-galactosamine (galnac) to serine or threonine residues in mucin core polypeptides by udpgalnac:polypeptide n-acetylgalactosaminyltransferases (ppgalnac-ts). it is not well understood how galnac attachment is regulated by multiple ppgalnac-ts in each cell. in the present study, the expression levels of murine ppgalnac-ts (mgalnac-ts), t1, t2, t3, t4, t6, and t7 were compared between mouse colon carcinoma colon 38 cells and vari ... | 2010 | 20077002 |
| the effect of alcohol consumption on later obesity in early adulthood--a population-based longitudinal study. | the study aimed to determine whether alcohol use during late adolescence contributes to the weight gain from adolescence to young adulthood or risk of obesity or waist circumference at young adulthood. | 2010 | 20071348 |
| bacteriophage adsorption efficiency and its effect on amplification. | existing models for bacteriophage adsorption are modified with the addition of a new term, adsorption efficiency, and applied to a t4-escherichia coli system. the adsorption efficiency is the fraction of phage that adsorbs irreversibly to the host. adsorption kinetics were modeled using the adsorption rate constant(k) and the adsorption efficiency(epsilon). experimental data demonstrated that the adsorption rate constant depends strongly on the condition of the host while the adsorption efficien ... | 2010 | 20066440 |
| phenotypic plasticity in number of glomeruli and sensory innervation of the antennal lobe in leaf-cutting ant workers (a. vollenweideri). | in the leaf-cutting ant atta vollenweideri, the worker caste exhibits a pronounced size-polymorphism, and division of labor is dependent on worker size (alloethism). behavior is largely guided by olfaction, and the olfactory system is highly developed. in a recent study, two different phenotypes of the antennal lobe of atta vollenweideri workers were found: mg- and rg-phenotype (with/without a macroglomerulus). here we ask whether the glomerular numbers are related to worker size. we found that ... | 2010 | 20029932 |
| post-treatment evaluation of a magnetic activator device in class ii high-angle malocclusions. | summary: the purpose of this study was to evaluate changes in dentofacial growth before, during, and after use of a magnetic activator device (mad) ii in high-angle class ii division 1 patients. lateral cephalometric and hand-wrist radiographs of 10 patients (six girls and four boys) with an average chronological age of 10 years 5 months were obtained at the beginning of the observation period (t1, 9.5 months), beginning of mad ii application (t2), at the end of the treatment (t3), and approxima ... | 2010 | 20053717 |
| frequency of hypothyroidism in patients of beta-thalassaemia. | to determine the frequency of hypothyroidism in patients suffering from homozygous beta-thalassaemia. | 2010 | 20055273 |
| exploration of composted cereal waste and poultry manure for soil restoration. | poultry manure and fungal consortium developed by including fungi of genus aspergillus, trichoderma and phanerochaete were evaluated separately to degrade high silica paddy straw. the quality evaluation of composted organic wastes after two months of decomposition revealed that poultry manure amended paddy straw compost had a c:n ratio of 13.06 and germination index of 132%, compared to 15.29 and 114% of its fungal inoculated counterpart. supplementation of paddy straw with poultry manure was as ... | 2010 | 20056413 |
| genotyping of acanthamoeba isolates and clinical characteristics of patients with acanthamoeba keratitis in china. | acanthamoeba keratitis (ak) is a sight-threatening corneal infection, the epidemiology of which is related to the specific genotype of acanthamoeba. in this study, the genotypes of 14 acanthamoeba isolates, each from a patient with ak, were identified according to the highly variable df3 region in the 18s rrna gene at shandong eye institute, pr china, from 2000 to 2009, and the clinical characteristics of these patients were analysed. all 14 amoebae were genotype t4, representing nine different ... | 2010 | 20056772 |
| spinal cord anaplastic oligodendroglioma with 1p deletion: report of a relapsing case treated with temozolomide. | primary spinal cord oligodendroglial tumor is very rare, and in only one patient with spinal cord oligodendroglioma has a deletion of chromosome 1p/19q been reported. we present the case of an 18-year-old girl, who had one-year lower back pain and one-month lower limb weakness. magnetic resonance images of the spinal cord showed an intramedullary mass from level t8 to t10, which was then radically removed. histology revealed an anaplastic oligodendroglioma. the patient was treated with radiother ... | 2010 | 21190059 |
| overexpression of cullin1 is associated with poor prognosis of patients with gastric cancer. | cullin1 (cul1) is a scaffold protein of the ubiquitin e3 ligase skp1/cullin1/rbx1/f-box protein complex, which ubiquitinates a broad range of proteins involved in cell-cycle progression, signal transduction, and transcription. to investigate the role of cullin1 in the development of gastric cancer, we examined the expression of cullin1 in primary gastric cancer tissues and analyzed the correlation between cullin1 expression and clinicopathologic variables and patients survival. we constructed a ... | 2010 | 21190721 |
| [thyroid dysfunction in primary care medicine]. | thyroid function tests include the measuring of the thyroid stimulating hormone (tsh) and free thyroxine (t4) in the case of abnormal tsh. these tests are frequently performed in primary care medicine since many clinical situations can be suggestive of dysthyroidism, as for example fatigue, depressive states or cardiac arthmia. in the case of subclinical thyroid dysfunction, the indications for treatment are controversial there being a lack of significant randomised studies. for primary care phy ... | 2010 | 21207724 |
| beyond translational research from t1 to t4: beyond “separate but equal” to integration (ti). | 2010 | 21207873 | |
| [total thyroidectomy: indications and complications]. | to investigate the indications and complications of the total thyroidectomy in the management of thyroid cancer. | 2010 | 21215203 |
| age aspects of demography, pathology and survival assessment in colorectal cancer. | the aim of this study was to assess how age is related to differences in stage, tumour differentiation and treatment in colorectal cancer. | 2010 | 21187518 |
| novel bacterial nad+-dependent dna ligase inhibitors with broad-spectrum activity and antibacterial efficacy in vivo. | dna ligases are indispensable enzymes playing a critical role in dna replication, recombination, and repair in all living organisms. bacterial nad+-dependent dna ligase (liga) was evaluated for its potential as a broad-spectrum antibacterial target. a novel class of substituted adenosine analogs was discovered by target-based high-throughput screening (hts), and these compounds were optimized to render them more effective and selective inhibitors of liga. the adenosine analogs inhibited the liga ... | 2010 | 21189350 |
| cetuximab in the treatment of head and neck cancer: preliminary results outside clinical trials. | the purpose of this study was to evaluate the clinical efficacy in our daily practice, outside clinical trials, of cetuximab plus radiotherapy in a majority of treatment-naive patients with locoregionally advanced head and neck squamous cell carcinomas. | 2010 | 21188107 |
| hypothyroidism in noninterferon treated-hcv infected individuals is associated with abnormalities in the regulation of th17 cells. | hcv-ag-specific th17 cells secrete il17, a cytokine involved in autoimmune diseases and regulated by il10 and tgf-b. 5-12% of patients with chronic hcv infection have hypothyroidism. we evaluated the role of these cytokines in this patients by determining serum concentration of tsh, t3, free t4, il2, il10, il12, il17, tgf-b, anti-tg, tpo, ccp, gbm, and cardiolipin antibodies in 87 chronically noninterferon treated hcv-infected patients. 20 patients (group a) had elevated tsh values (>5 μui/ml) w ... | 2010 | 21188205 |
| preoperative radiotherapy with capecitabine and mitomycin c in locally advanced rectal carcinoma. | purpose: to evaluate the efficacy and safety of preoperative radiotherapy with capecitabine and mitomycin c in patients with locally advanced rectal cancer. methods: a prospective, open-label, non-randomized, phase ii study was performed on 49 patients with locally advanced rectal cancer. preoperative radiotherapy was conducted on linear accelerators (15 or 18 mv) with a tumor dose of 45 gy in 25 fractions over 5 weeks, combined with mitomycin c 7 mg/m(2) on days 1 and 29 and oral capecitabine 8 ... | 2010 | 21188380 |
| serum free triiodothyronine (t3) to free thyroxine (t4) ratio in treated central hypothyroidism compared with primary hypothyroidism and euthyroidism. | the standard treatment of hypothyroidism (central and primary) consists of thyroxine (t4) administration alone. however, the normal thyroid gland produces a small proportion of triiodothyronine (t3) directly into the circulation. | 2010 | 21195684 |
| sentinel lymph node biopsy status is a key parameter to stratify the prognostic heterogeneity of malignant melanoma in high-risk tumors >4.0 mm. | the value of sentinel lymph node biopsy (slnb) as a useful strategy to assess the risk of future metastasis in high-risk melanomas (>4.0 mm) is controversially discussed. | 2010 | 21196701 |
| [operable colon cancer: initial strategy]. | individual screening of patients with personal or family history of colon cancer or polyps, or patients with an inflammatory condition of the digestive tract, combined with the generalization of mass screening using hemoccult, have modified the way colon cancer is diagnosed. the optimization of colon surgery, together with adjuvant chemotherapy, has improved the 5- and 10-year survival. the five-year survival rate is now comprised between 90 percent in patients with stage-i colon cancer, and 65- ... | 2010 | 21197740 |
| prognostic factors for elderly breast cancer patients in university malaya medical centre, malaysia. | information about elderly breast cancer patients' outcome is limited. this study aimed to evaluate the treatment outcomes in women aged 70 and above with specific analysis on prognostic clinicopathological features and treatment modalities. | 2010 | 21198264 |
| [relationship between beta amyloid protein 1-40 and post-operative delirium after oral and maxillofacial surgery in elderly patients]. | to determine the incidence of post-operative delirium after oral and maxillofacial surgery under general anesthesia in elderly patients, and to examine its association with plasma concentrations of beta amyloid protein 1-40 (abeta1-40). | 2010 | 21179683 |
| vulnerability of healthy vertebrae in patients with and without previous vertebral fracture. | vertebral deformities are associated with a marked increase in morbidity, mortality, and burden in terms of sanitary expenditures. patients with vertebral fractures have a negative impact in their health, less quality of life, and loss of functional capacity and independence. the purpose of this study was to explore the vulnerability of healthy vertebrae in patients who have sustained already a compression fracture and in patients who do not have prevalent fractures in the thoracic spine; and to ... | 2010 | 21185414 |
| delayed colo-anal anastomosis is an alternative to prophylactic diverting stoma after total mesorectal excision for middle and low rectal carcinomas. | after total mesorectal excision (tme), a low colorectal or colo-anal anastomosis is usually performed. a prophylactic covering stoma is often used, especially for patients receiving neoadjuvant chemo-radiotherapy. however, morbidity is high, mainly due to anastomotic leakage. | 2010 | 21186091 |
| thyroid function in pregnancy. | advances in understanding the physiology of thyroid function in normal pregnancy have highlighted the importance of the consequences of abnormal function on obstetric outcome and foetal well-being. pubmed search was done using the terms thyroid and pregnancy. areas of agreement are the following: gestational normative reference ranges for thyroid function tests are required for proper interpretation of any abnormalities. measurement of thyroid-stimulating antibodies and antithyroid peroxidase an ... | 2010 | 21186204 |
| effects of acute normovolemic hemodilution on perioperative coagulation and fibrinolysis in elderly patients undergoing hepatic carcinectomy. | to observe the effects of acute normovolemic hemodilution (anh) on coagulation function and fibrinolysis in elderly patients undergoing hepatic carcinectomy. | 2010 | 21180275 |
| prognostic analysis in node-negative gastric cancer patients in china. | gastric cancer patients with negative nodes were considered to have better outcomes, however, some of them still suffered from disease recurrences or distant metastases after radical resection. a total of 1,020 gastric carcinoma patients receiving treatment in our center between 2003 and 2008 were selected for the analysis. all patients received gastrectomy and d2 lymphadenectomy. survival analysis was performed with cox regression model. the final study includes 222 patients. the overall 5-year ... | 2010 | 21181465 |
| prevalence of depression in patients affected by subclinical hypothyroidism. | the aim of this study was to estimate the prevalence of depressive symptoms in patients affected by subclinical hypothyroidism by means of hamilton rating scale for depression (ham-d) and montgomery-asberg depression rating scale (madrs); to assess which depressive symptoms are prevalent in our population, with references to the factorialization of ham-d by cleary and guy (1977); to verify whether levothyroxine replacement therapy alone can induce total remission of depressive symptoms. | 2010 | 21183887 |
| postoperative radiotherapy in head and neck mucosal melanoma: a gettec study. | to report patterns of failure according to treatment modality, with an emphasis on the role of postoperative radiotherapy in patients with localized head and neck mucosal melanoma (hnmm) treated during a 28-year period in a multi-institutional setting. | 2010 | 21173371 |
| transoral robotic-assisted surgery for head and neck squamous cell carcinoma: one- and 2-year survival analysis. | to report 2-year survival outcomes for head and neck squamous cell carcinoma using transoral robotic-assisted resection. | 2010 | 21173375 |
| hormone replacement after thyroid and parathyroid surgery. | hypothyroidism and hypocalcemia are common after thyroid and parathyroid surgery. in this article, the authors provide clinically-oriented recommendations to help surgeons, general practitioners, internists, and endocrinologists give their affected patients adequate hormone replacement therapy. | 2010 | 21173898 |
| anticoccidial effects of coumestans from eclipta alba for sustainable control of eimeria tenella parasitosis in poultry production. | the aim of this study was to evaluate the anticoccidial efficacy of a product containing coumestans from eclipta alba. experimental conditions were set up as to reproduce the environment conditions for husbandry adopted in commercial broiler farms. broilers were raised in broiler chicken shed provided with feeders, drinkers, illumination and temperature control systems and floor covering to afford an adequate nourishing environment. male cobb broilers (240) were assigned to four experimental gro ... | 2010 | 21177038 |
| [recurrence of cancer of the paranasal sinuses after primary treatment--analysis of conditions and results of salvage treatment]. | the aim of this study: is to demonstrate epidemiological and clinical parameters of the group of patients with sinonasal malignancies and to analyze its impact on development of recurrences after primary surgical treatment conducted in head and neck surgery department of holly cross cancer center kielce during 7-years period 2001-2007. the retrospective analysis of the group of 42 patients with sinonasal malignancies was made, based on medical record and outpatient follow-up, considering: age, s ... | 2010 | 21171311 |
| [accelerated postoperative radiotherapy in patients with advanced larynx cancer]. | the aim of study was test efficacy of accelerated postoperative radiotherapy--concomitant boost in patients with advanced larynx cancer. | 2010 | 21171314 |
| persistence of bacteriophage t4 in a starved escherichia coli culture: evidence for the presence of phage subpopulations. | bacteriophage t4 is able to adjust its development to the growth parameters of the host cell. here, we present evidence for the production of two different subpopulations of phage particles, which differ in their ability to infect starved escherichia coli cells. the ability of phage t4 to produce a fraction of virions unable to infect starved cells is linked to the functions of genes ri and riii, as well as riia. this may represent the adaptation of phage t4 in order to persist in unfavourable e ... | 2010 | 21177930 |
| anchoring the type vi secretion system to the peptidoglycan: tssl, tagl, tagp... what else? | the recently identified bacterial type vi secretion system (t6ss) has rapidly become one of the most interesting areas of research in microbiology. in a relatively short period of time the relationship between the t6ss and the bacteriophage t4 tail and baseplate has been established. however, a number of questions concerning the t6ss remain the focus of a large number of researchers worldwide. key questions that need to be addressed include how this system assembles in the cell envelope and the ... | 2010 | 21178498 |
| sentinel node biopsy in colorectal cancer: must we believe it? | lymph node metastasis predicts survival and recurrence in colon cancer (cc), so decisions regarding adjuvant therapy are largely based on nodal status. chemotherapy is not a routine treatment for node-negative cc because its toxicity and expense exceed its limited benefit in patients without evidence of nodal involvement. the sentinel lymph node (sln) procedure is a selective sampling technique that can be used to ultrastage regional nodes. the real problem of sln biopsy in cc is the procedure s ... | 2010 | 21160827 |