Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| use of a whole chromosomal probe for identification of treponema hyodysenteriae. | a whole chromosomal dna probe labelled with photobiotin was used in a dot blot hybridisation to identify dna from isolates of treponema hyodysenteriae, the aetiological agent of swine dysentery. the probe was evaluated using dna from 13 isolates of t hyodysenteriae and 13 isolates of non-t hyodysenteriae spirochaetes recovered from pigs. the initial test had both a sensitivity and specificity of 92.3 per cent, although when it was repeated the specificity fell to 84.6 per cent. the test was help ... | 1991 | 1882134 |
| analysis of outer membrane ultrastructure of pathogenic treponema and borrelia species by freeze-fracture electron microscopy. | we analyzed the outer membrane (om) ultrastructure of four pathogenic members of the family spirochaetaceae by freeze fracture. the om of treponema pallidum subsp. pertenue contained a low intramembranous particle concentration, indicating that it contains few om transmembrane proteins. the concave om fracture faces of treponema hyodysenteriae and borrelia burgdorferi contained dense populations of particles, typical of gram-negative organisms. a relatively low concentration of particles which w ... | 1991 | 1885536 |
| slide-agglutination for rapid serological typing of treponema hyodysenteriae. | a slide agglutination (sa) test was developed to determine the serogroup of isolates of treponema hyodysenteriae of serogroups a to f. rabbit antisera which are normally used for serogrouping t. hyodysenteriae in an agarose gel double-diffusion precipitation test (agdp) were not suitable for sa because they agglutinated isolates from more than one serogroup. the agglutination reaction was made serogroup-specific by cross-absorbing the typing sera for serogroups a to f with whole treponemes from ... | 1991 | 1904826 |
| identification of treponema hyodysenteriae and treponema innocens using two four-hour identification systems. | two 4-hour identification systems, the rapid-anaii (innovative diagnostic systems) and the ani card (vitek systems), were used to identify isolates of treponema hyodysenteriae and t. innocens. twenty-one isolates of t. innocens and 53 isolates of t. hyodysenteriae were tested with both systems. with the ani system, alpha-galactosidase was the only test that differentiated the two species. with the rapid-anaii system, alpha-galactosidase and indole tests allowed differentiation of the two species ... | 1991 | 1911990 |
| phylogenetic analysis of the spirochetes. | the 16s rrna sequences were determined for species of spirochaeta, treponema, borrelia, leptospira, leptonema, and serpula, using a modified sanger method of direct rna sequencing. analysis of aligned 16s rrna sequences indicated that the spirochetes form a coherent taxon composed of six major clusters or groups. the first group, termed the treponemes, was divided into two subgroups. the first treponeme subgroup consisted of treponema pallidum, treponema phagedenis, treponema denticola, a thermo ... | 1991 | 1917844 |
| role of intestinal excretion in the effect of subcutaneously administered sedecamycin on cecal infection caused by treponema hyodysenteriae in mice. | the therapeutic effects of subcutaneously administered sedecamycin on experimental treponema hyodysenteriae infection in mice were evaluated. sedecamycin was more active than tiamulin and lincomycin. the efficacy of sedecamycin upon subcutaneous administration was similar to that upon oral administration. sedecamycin given subcutaneously provided similar degrees of protection in bile duct-ligated and intact mice. pharmacokinetic studies utilizing a liquid chromatographic technique were carried o ... | 1991 | 1929332 |
| application and evaluation of enzyme-linked immunosorbent assay and immunoblotting for detection of antibodies to treponema hyodysenteriae in swine. | an enzyme-linked immunoassay (elisa) has been developed to detect serum immunoglobulin antibodies g and m to treponema hyodysenteriae in vaccinated, experimentally infected and naturally infected swine. naturally infected swine gave elisa titres that were similar to experimentally infected swine, but were significantly less than the titres of vaccinated swine. when serum from naturally infected swine was used to probe nitrocellulose blots of sodium dodecyl sulphate-polyacrylamide gel electrophor ... | 1991 | 1936151 |
| isolation of treponema hyodysenteriae from a wild rat living on a piggery. | 1991 | 1953567 | |
| a cytotoxic haemolysin from treponema hyodysenteriae--a probable virulence determinant in swine dysentery. | the haemolysin from a virulent strain of treponema hyodysenteriae was extracted and injected into ligated loops of the ileum and colon of germ-free pigs. it caused severe epithelial damage, especially to the differentiated cells at the tips of the villi in the ileum and the cells in the intercrypt zones of the colon; goblet cells were less affected. the changes in the colon were similar to those seen in natural cases of swine dysentery. the ligated loop offers a means of investigating pathogenic ... | 1991 | 1990146 |
| molecular characterization of serpulina (treponema) hyodysenteriae isolates representing serotypes 8 and 9. | the study described here was carried out to further characterize reference strains of serpulina (treponema) hyodysenteriae representing serotypes 8 and 9. results obtained from restriction fragment length polymorphism analysis, enteropathogenicity testing, and endotoxin profiles confirmed their identifications. electron microscopy indicated that both strains were covered with a thin layer of capsule-like material. immunoblot analysis indicated that an antigen in the 19-kda region of proteinase k ... | 1992 | 1280646 |
| morphologic analysis of enteric lesions in conventional and streptomycin-treated inbred c3h/hen mice infected with serpulina (treponema) hyodysenteriae. | oral administration of streptomycin is known to enhance the susceptibility of mice to enteric pathogens by altering the indigenous flora. we examined the effect of oral streptomycin treatment on the susceptibility of inbred c3h/hen mice to infection with serpulina (treponema) hyodysenteriae. a total of 56 mice were randomly divided into four groups (a-d) of 14 each. from days 0 to 7, mice in groups a and b received streptomycin in their drinking water and mice in groups c and d served as control ... | 1992 | 1316514 |
| characterization of dutch porcine serpulina (treponema) isolates by restriction endonuclease analysis and dna hybridization. | genomes of 55 dutch porcine serpulina (treponema) hyodysenteriae and non-pathogenic serpulina isolates were characterized by restriction endonuclease analysis (rea) and dna hybridization. the dutch porcine isolates were compared with american type culture collection (atcc) strains of s. hyodysenteriae and s. innocens and isolates of s. hyodysenteriae with known serotypes (reference strains). rea of the dutch s. hyodysenteriae isolates resulted in two main patterns, while the non-pathogenic isola ... | 1992 | 1328472 |
| characterization of serpulina (treponema) hyodysenteriae and related intestinal spirochetes by ribosomal rna gene restriction patterns. | serpulina (treponema) hyodysenteriae strain a-1 partially purified rrna, labelled with photobiotin, was used as a non-radioactive probe to identify the rrna gene restriction patterns of s. hyodysenteriae strains and other spirochetes. sau3a restriction enzyme digests resulted in similar rrna gene restriction patterns in s. hyodysenteriae strains from five different countries. some s. hyodysenteriae strains could be differentiated by variations in their rrna gene restriction patterns after cleava ... | 1992 | 1354196 |
| the serological grouping system for serpulina (treponema) hyodysenteriae. | lipopolysaccharide from serostrains of serpulina (treponema) hyodysenteriae for serogroups a to i was characterized using sodium dodecylsulphate polyacrylamide gel electrophoresis and silver staining. all strains had lipopolysaccharide components ranging from 10 to 16 kda that represented lipid a-core polysaccharide regions, and short o-antigen side chain were also recognized in certain immunoblots. serological reactions between lipopolysaccharide and antisera against each of these serostrains w ... | 1992 | 1397114 |
| virginiamycin susceptibility of serpulina hyodysenteriae, in vitro and in vivo. | 1992 | 1441115 | |
| virulent serpulina hyodysenteriae from a pig in a herd free of clinical swine dysentery. | 1992 | 1441137 | |
| spirochete chemotaxis, motility, and the structure of the spirochetal periplasmic flagella. | spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (pf) rotate in several spirochetal species. this rotation generates thrust. as shown for one spirochete, spirochaeta aurantia, motility is driven by a proton motive force. spirochete chemotaxis has been most thoroughly studied in s. aurantia. this spiroch ... | 1992 | 1475520 |
| interaction of spirochetes with the host. | the success of an invading organism must depend on several cytoplasmic, surface-associated and secreted factors. the technical difficulties in handling pathogenic spirochetes like treponema pallidum and borrelia burgdorferi have made it difficult to define specific factors involved in entry and long-term survival. the problem of defining virulence factors has been attacked by several strategies: t. pallidum secretes a number of immunogenic low molecular mass proteins. the most predominant are of ... | 1992 | 1475523 |
| a camp-like phenomenon in serpulina (treponema) spp. | a camp-like phenomenon was observed in 40 serpulina (treponema) isolates from the intestines of pigs, dogs, mice and rats. the strains examined included reference strains of the serotypes 1-4 of serpulina (treponema) hyodysenteriae and serpulina (treponema) innocens. the possible pathogenic importance of this phenomenon is discussed. | 1992 | 1486230 |
| the periplasmic flagella of serpulina (treponema) hyodysenteriae are composed of two sheath proteins and three core proteins. | the major components of the periplasmic flagella of the spirochaete serpulina (treponema) hyodysenteriae strain c5 were purified and characterized. we demonstrate that the periplasmic flagella are composed of five major proteins (molecular masses 44, 37, 35, 34 and 32 kda) and present their location, n-terminal amino acid sequence and immunological relationship. the 44 kda and the 35 kda protein are on the sheath of the periplasmic flagellum, whereas the 37, 34 and 32 kda protein reside in the p ... | 1992 | 1487733 |
| comparison of six commercially available transport media for maintenance of serpulina (treponema) hyodysenteriae. | two anaerobic (a1 and a2), 1 selective (s1), and 3 conventional (c1, c2, and c3) transport media formulations were compared for their capacity to maintain the viability of serpulina (treponema) hyodysenteriae. initial experiments compared the recovery of s. hyodysenteriae from pure cultures held in each transport medium for 0.5, 1, 2, 3, 5, and 7 days at -40 c, 4 c, 25 c, and 36 c. subsequent experiments compared each transport medium for maintenance of s. hyodysenteriae in fecal specimens obtai ... | 1992 | 1515490 |
| monoclonal antibodies to a 16-kda antigen of serpulina (treponema) hyodysenteriae. | monoclonal antibodies (mabs) were produced to an outer-envelope preparation from serpulina (treponema) hyodysenteriae, the aetiological agent of swine dysentery. three mabs (isotype igg1) were obtained. all three recognised a 16-kda antigen that was common to most strains of s. hyodysenteriae of different serotypes but was absent from nonpathogenic, porcine intestinal spirochaetes. immunofluorescence and immunogold labelling studies showed that the 16-kda antigen was exposed on the surface of in ... | 1992 | 1518039 |
| cp-82,009, a potent polyether anticoccidial related to septamycin and produced by actinomadura sp. | a new polyether antibiotic cp-82,009 (c49h84o17) was isolated by solvent extraction from the fermentation broth of actinomadura sp. (atcc 53676). following purification by column chromatography and crystallization, the structure of cp-82,009 was elucidated by spectroscopic (nmr and ms) methods. the absolute stereochemistry was determined by a single crystal x-ray analysis of the corresponding rubidium salt. cp-82,009 is among the most potent anticoccidial agents known, effectively controlling th ... | 1992 | 1577662 |
| a serological survey to determine the prevalence of infection with treponema hyodysenteriae in western australia. | a serological survey to detect antibody titres against treponema hyodysenteriae was conducted on pigs from 106 herds in western australia. titres indicating a positive result in the tests were determined by examining 400 sera from 4 herds known to be free of swine dysentery, and sera from immunised or experimentally infected pigs. samples of serum from 40 bacon-weight pigs from each of the 106 herds were then collected at 2 abattoirs. each serum was tested in enzyme-linked immunosorbent assays ( ... | 1992 | 1605788 |
| cloning and dna sequence analysis of a serpulina (treponema) hyodysenteriae gene encoding a periplasmic flagellar sheath protein. | a serpulina (treponema) hyodysenteriae expression library was constructed in vector lambda zap and screened with a polyclonal antiserum raised against s. hyodysenteriae periplasmic flagella. a single immunoreactive plaque was chosen for further analysis. the recombinant phage from this plaque contained a gene encoding the 44-kda protein that is on the outer layer (or sheath) of the periplasmic flagella. dna sequence analysis showed that the gene encodes a protein of 320 amino acids. the protein ... | 1992 | 1612759 |
| serodiagnosis of leptospirosis in pigs using an axial filament enzyme-linked immunosorbent assay. | the axial filament (af) from leptospira interrogans serovar canicola was isolated by cesium chloride density gradient centrifugation of 2% sarcosyl treated whole cells. isolation of af was confirmed by electron microscopic examination, by protein-a immunogold labelling, sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page), and immunoblotting. analysis by sds-page of the purified preparation showed relatively weak bands of molecular size 41 kda and 21 kda, and strong bands of 35 ... | 1992 | 1615635 |
| inactivation of a serpula (treponema) hyodysenteriae hemolysin gene by homologous recombination: importance of this hemolysin in pathogenesis in mice. | 1992 | 1624107 | |
| typing of australian isolates of treponema hyodysenteriae by serology and by dna restriction endonuclease analysis. | a total of 91 isolates of treponema hyodysenteriae which were obtained from 62 piggeries located around australia were typed by serology and by dna restriction endonuclease analysis (rea). the isolates fell into eight serogroups, of which groups b and d were the most common. isolates with different rea patterns were recognised within serogroups, whilst a few isolates with the same rea pattern were placed into different serogroups. some of the latter isolates were either from the same piggery or ... | 1992 | 1626376 |
| a 16-kilodalton lipoprotein of the outer membrane of serpulina (treponema) hyodysenteriae. | serpulina (treponema) hyodysenteriae p18a and vs1 were extracted by using the detergent triton x-114 and separated into detergent and aqueous phases. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western immunoblot analysis confirmed that a membrane-associated 16-kda antigen was hydrophobic, since it was found in the detergent phase. a 45-kda antigen partitioned into the aqueous phase, suggesting that it was hydrophilic and may be of periplasmic origin. when spirochetes were grow ... | 1992 | 1639479 |
| an enhanced murine model for studies of serpulina (treponema) hyodysenteriae pathogenesis. | a defined diet was used to increase the susceptibility of mice to serpulina hyodysenteriae. balb/cbyj, c3h/hen, and c3h/hej mice, when fed the defined diet 7 to 14 days prior to and throughout the challenge period, consistently showed higher incidences of disease than mice maintained on normal rodent chow. the use of this defined diet will increase the consistency of in vivo studies following infection with s. hyodysenteriae in the mouse model. | 1992 | 1639511 |
| molecular cloning and characterization of dna from human intestinal spirochetes. | hindiii cleaved human intestinal spirochete genomic dna was cloned into the plasmid vector pbluescript. the inserts from three of these clones (prb.c33, prb.c47 and prb.f11) successfully hybridized with dna from the nine human intestinal spirochetes used in this study. the fourth insert (from clone prb.a8) failed to hybridize with dna from two of these isolates (hrm4 and hrm9), and, all four of the inserts hybridized with the m1 strain of swine intestinal spirochete treponema innocens. hybridiza ... | 1992 | 1644136 |
| cloning and expression of a serpula (treponema) hyodysenteriae hemolysin gene. | serpula (treponema) hyodysenteriae, the etiologic agent of swine dysentery, produces a hemolysin which is thought to be an important factor in the pathogenesis of the disease. we report the cloning, sequencing, and expression of a hemolysin gene (tly) from s. hyodysenteriae b204. a puc19 gene bank of strain b204 was constructed in the escherichia coli k-12 strain dh5 alpha, and hemolytic recombinants were identified by plating the library on blood agar plates. from the hemolytic recombinants, a ... | 1992 | 1730486 |
| comparison of six different culture media for isolation of treponema hyodysenteriae. | fecal material from pigs experimentally infected with treponema hyodysenteriae was collected and inoculated on six different selective media to compare their abilities for recovery of t. hyodysenteriae. additionally, various types of samples were compared to find the most appropriate for submitting material to laboratories. isolation rates for t. hyodysenteriae were 89.7% on bj medium, 88.3% on tsa-bj medium, 76.6% on svc medium, 76.6% on ba2-bj medium, 75.2% on ba2-svc medium, and 52.4% on tsa- ... | 1992 | 1734063 |
| proposal to change the genus designation serpula to serpulina gen. nov. containing the species serpulina hyodysenteriae comb. nov. and serpulina innocens comb. nov. | the bacterial genus serpula stanton et al. 1991 is illegitimate due to the existence of a fungal genus serpula pers. ex s. f. gray. consequently, a new genus designation, serpulina, is proposed for this spirochete genus. serpula hyodysenteriae, the type species, and serpula innocens stanton et al. 1991, therefore, become serpulina hyodysenteriae comb. nov. and serpulina innocens comb. nov. | 1992 | 1736966 |
| the use of 16s rdna sequence analysis to investigate the phylogeny of leptospiraceae and related spirochaetes. | the 16s rdna sequences from 15 leptospiraceae were determined by automated pcr-directed cycle sequencing. nucleotide comparisons, including those from published sequences for leptospira canicola moulton and serpulina spp., were used to construct phylogenetic trees. serpulina hyodysenteriae and s. innocens were related to each other but were distinct from the leptospiraceae comprising leptospira parva incertae sedis (turneria parva h), leptonema illini and leptospira spp. the pathogenic and the s ... | 1993 | 7506293 |
| genetic similarity of intestinal spirochetes from humans and various animal species. | the chromosomal dna of spirochetes isolated from human, swine, dog, mouse, rat, and chicken intestine or feces was subjected to restriction enzyme analysis and hybridization with three different dna probes, derived from a flagellin gene, a hemolysin gene, and the 16s rdna sequence of the pathogenic swine intestinal spirochete serpulina hyodysenteriae. this genetic analysis showed that intestinal spirochetes represent a heterogeneous but related population of bacteria. in general, unique genotype ... | 1993 | 8096218 |
| comparison of serpulina hyodysenteriae b78, the type strain of the species, with other s. hyodysenteriae strains using enteropathogenicity studies and restriction fragment length polymorphism analysis. | the enteropathogenicity of serpulina hyodysenteriae b78, the type strain of the species, was compared with s. hyodysenteriae b204, a known pathogenic strain, in 7 week-old pigs. clinical signs of swine dysentery were observed in 11/18 pigs (61.1%) inoculated with s. hyodysenteriae strain b204. however, in pigs inoculated with s. hyodysenteriae b78, only 1/21 (4.8%) of the pigs became infected. the 21 pigs inoculated with strain b78 included four pigs which received 5-fold higher numbers of s. hy ... | 1993 | 7903832 |
| 16 kda envelope proteins in non-serpulina hyodysenteriae spirochaetes isolated from pigs. | spirochaetes isolated from field samples of diarrhoea, 'colitis' and mucoid diarrhoea from pigs were examined by a series of cultural, biochemical and serological tests. in addition sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to determine whether the organisms possessed a 16 kda protein thought to distinguish serpulina hyodysenteriae from s innocens. spirochaetal isolates which differed culturally and biochemically from s hyodysenteriae were found to possess a 16 kda prot ... | 1993 | 8236639 |
| cross-reactivity between b. burgdorferi and other spirochetes affects specificity of serotests for detection of antibodies to the lyme disease agent in dogs. | western immunoblots, the kinetics-based enzyme-linked immunosorbent assay (kela), and the microagglutination test were used to evaluate cross-reactivity among antibodies to serovars of leptospira interrogans (leptospiral serovars), and b. burgdorferi from naturally infected dogs, and to serpulina (treponema) hyodysenteriae from vaccinated rabbits. whole-cell lysates from borrelia spp., leptospiral serovars, and serpulina spp. were used for sds-page, western blots, and kela. crossreactivity occur ... | 1993 | 8236777 |
| a species-specific periplasmic flagellar protein of serpulina (treponema) hyodysenteriae. | we have previously reported that a 46-kda protein present in an outer membrane protein preparation seemed to be a species-specific antigen of serpulina hyodysenteriae (z. s. li, n. s. jensen, m. bélanger, m.-c. l'espérance, and m. jacques, j. clin. microbiol. 30:2941-2947, 1992). the objective of this study was to further characterize this antigen. a western blot (immunoblot) analysis and immunogold labeling with a monospecific antiserum against this protein confirmed that the protein was presen ... | 1993 | 8253687 |
| identification and partial characterization of a group of weakly beta-hemolytic intestinal spirochetes of swine distinct from serpulina innocens isolate b256. | comparative analyses of a group of 16 weakly beta-hemolytic spirochetes isolated from feces and mucosal scrapings of intestines of swine in the midwestern united states, and eastern canada revealed the existence of a phenotypically and genotypically related group of 7 isolates. although isolates in this group differed from all known reference isolates of intestinal spirochetes of swine, partial similarity was detected with s. joneseae isolate 16, a newly identified weakly beta-hemolytic intestin ... | 1993 | 8296452 |
| the role of hemolysin(s) in the pathogenesis of serpulina hyodysenteriae. | serpulina (treponema) hyodysenteriae, an anaerobic beta hemolytic spirochaete, is the etiologic agent of swine dysentery. not much is known at present about the virulence factors of s. hyodysenteriae. however, the hemolysin production of this bacterium is generally accepted to be a virulence factor. to study the exact role of hemolysin production in the pathogenesis of swine dysentery, the gene encoding a hemolysin, tly, was cloned and its nucleotide sequence determined. after inactivation of th ... | 1993 | 8347935 |
| characterization of two dna probes specific for serpulina hyodysenteriae. | two dna probes, one 1.1- and one 0.75-kb probe, specific for serpulina hyodysenteriae were isolated from a genomic library generated from virulent s. hyodysenteriae 5380. these probes are highly specific and react with all s. hyodysenteriae strains tested. under stringent conditions, the dna probes did not react with the nonpathogenic species serpulina innocens or with other species of enteric bacteria, including escherichia coli. both probes are able to detect s. hyodysenteriae in colony blot h ... | 1993 | 8349750 |
| comparison of outer-membrane fractions of serpulina (treponema) hyodysenteriae. | sarkosyl-insoluble fractions (outer-membrane proteins) and endoflagella (ef) fractions of serpulina hyodysenteriae serotypes 1-7 were examined for protein differences using sds-page. both the outer-membrane proteins (omp) and endoflagella were also examined for antigenicity using porcine sera from acutely infected and convalescent swine. seven major staining proteins were resolved in outer-membrane enriched fractions ranging in molecular weight between 42 and 32 kda. a comparison of purified ef ... | 1993 | 8362492 |
| genetic relationships between isolates of serpulina (treponema) hyodysenteriae, and comparison of methods for their subspecific differentiation. | multilocus enzyme electrophoresis (mee) was used to examine the extent of genetic diversity amongst 98 isolates of serpulina (treponema) hyodysenteriae. the species contained four major genetic divisions (a, b, c and d) and 29 electrophoretic types (ets). division d was relatively distinct, being separated from the other three divisions by fixed allelic differences at an average of 6.6 of 15 enzyme loci. electrophoretic differences were compared with results of dna restriction endonuclease analy ... | 1993 | 8383375 |
| molecular analysis of a flagellar core protein gene of serpulina (treponema) hyodysenteriae. | the flab2 gene encoding a protein located in the core of the periplasmic flagella of serpulina hyodysenteriae was cloned and sequenced. the flab2 protein consists of 285 amino acids and has a calculated molecular mass of 31.1 kda. southern blot analysis indicated that at least one, and possibly two genes related to flab2 are present in the genome of s. hyodysenteriae. comparison of the amino acid sequence of flab2 to sequences present in data banks showed significant similarity with the core fla ... | 1993 | 8409912 |
| human intestinal spirochetes are distinct from serpulina hyodysenteriae. | twenty-nine intestinal spirochetes isolated from australian aboriginal children and six strains from italian adults (hrm1, -2, -4, -5, -7, and -14) were genetically examined at 15 enzyme loci by using multilocus enzyme electrophoresis. results were compared with those previously obtained for 188 porcine intestinal spirochetes. dna from human strain hrm7 and porcine strain serpulina hyodysenteriae p18a were also radioactively labeled and hybridized with dna from 12 other human and porcine intesti ... | 1993 | 8417025 |
| molecular cloning, expression, and dna sequence analysis of the gene that encodes the 16-kilodalton outer membrane lipoprotein of serpulina hyodysenteriae. | the gene (smpa) that encodes the 16-kda outer membrane lipoprotein of serpulina hyodysenteriae was cloned in escherichia coli, and its primary structure was determined by nucleotide sequencing. the putative open reading frame encodes a prolipoprotein of 16.8 kda which in its fully acylated and cleaved form is 15.1 kda. analysis of the n-terminal amino acid sequence derived from the dna sequence revealed the presence of a signal sequence and a putative acylation and signal peptidase ii cleavage s ... | 1993 | 8432595 |
| experiences with a vaccine being developed for the control of swine dysentery. | a prototype vaccine that is being developed for the control of swine dysentery (sd) was tested in two groups of experimental pigs. vaccination induced high circulating antibody titres against the aetiological agent, serpulina (treponema) hyodysenteriae. pigs in the first trial were vaccinated twice before being challenged orally with the bacteria. five of 6 unvaccinated animals developed dysentery within a fortnight of challenge, but only 1 of 6 vaccinated pigs showed signs of disease at this ti ... | 1993 | 8460983 |
| purification and characterization of nadh oxidase from serpulina (treponema) hyodysenteriae. | nadh oxidase (ec 1.6.99.3) was purified from cell lysates of serpulina (treponema) hyodysenteriae b204 by differential ultracentrifugation, ammonium sulfate precipitation, and chromatography on anion-exchange, dye-ligand-affinity, and size-exclusion columns. purified nadh oxidase had a specific activity 119-fold higher than that of cell lysates and migrated as a single band during denaturing gel electrophoresis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis [sds-page]). the enzyme wa ... | 1993 | 8491717 |
| identification of a novel group of serpulina hyodysenteriae isolates by using a lipopolysaccharide-specific monoclonal antibody. | a monoclonal antibody to serpulina hyodysenteriae 8930 was produced and was used to probe pronase-treated cell lysates of s. hyodysenteriae isolates in immunblots. the results showed that the monoclonal antibody was specific for only five closely related s. hyodysenteriae isolates: 8930, 5380, 70a, rmit 88, and rmit 97. | 1993 | 8501237 |
| monitoring experimental swine dysentery: rectal swab blood test and serpulina (treponema) hyodysenteriae detection. | in two experiments, ten postweaning piglets were inoculated intragastrically with cultures of serpulina (treponema) hyodysenteriae strain b204 and were individually monitored for swine dysentery. eight control animals received sterile culture medium. between the day of inoculation and 35 days later, rectal swab samples were assayed for fecal blood by means of a commercial test kit and for s. hyodysenteriae cells by direct (phase contrast) microscopy and selective culture methods. optimal detecti ... | 1993 | 8506612 |
| comparison of the fatty acid profiles of borrelia, serpulina and leptospira species. | fatty acid methyl ester (fame) derivatives were examined as a means of characterizing borrelia burgdorferi isolates and distinguishing them from other spirochaetes. analysis was performed using a gas liquid chromatography column in conjunction with microbial identification system (mis) software. reproducible fame profiles were produced which distinguished borrelia species, serpulina hyodysenteriae and leptospira icterohaemorrhagiae. furthermore, the fame profiles of four recognized borrelia spec ... | 1993 | 8515244 |
| identification and characterization of serpulina hyodysenteriae by restriction enzyme analysis and southern blot analysis. | chromosomal dna restriction enzyme analysis and southern blot hybridization were used to characterize serpulina hyodysenteriae strains. when chromosomal dnas from selected strains (reference serotypes) of s. hyodysenteriae were digested with the restriction endonuclease sau3a and hybridized with a 1.1-kb s. hyodysenteriae-specific dna probe, a common 3-kb band was always detected in s. hyodysenteriae strains but was absent from serpulina innocens strains. when the chromosomal dna was digested wi ... | 1994 | 7914209 |
| production of an inducible sucrase activity by serpulina hyodysenteriae. | strains of serpulina hyodysenteriae and serpulina innocens produced a cell-associated sucrase activity when grown in a medium containing sucrose. s. hyodysenteriae b204 sucrase activity cleaved sucrose and, to a lesser extent, raffinose and had a ph optimum of 5.7 to 6.2. this is the first report of an inducible enzyme produced by either s. hyodysenteriae or s. innocens. | 1994 | 7944375 |
| antimicrobial susceptibility testing of serpulina hyodysenteriae. | the macrobroth dilution technique was used to test the in-vitro effectiveness of 4 commonly used antimicrobial agents against 23 australian isolates and 7 overseas strains of serpulina hyodysenteriae. minimum inhibitory concentrations and minimum bactericidal concentrations were determined. the growth of 90% of isolates was inhibited by dimetridazole at a concentration of 4 micrograms/ml, and by tiamulin at 8 micrograms/ml. australian isolates resistant to both antimicrobial agents were identifi ... | 1994 | 7945100 |
| characterization of three putative serpulina hyodysenteriae hemolysins. | serpulina hyodysenteriae hemolysin is though to be an important virulence factor in swine dysentery. one gene, tlya, previously called tly, encoding a hemolysin in s. hyodysenteriae strain b204 has been characterized (muir et al. infect immun 1992; 60: 529-35). two other genes of s. hyodysenteria strain b204, designated tlyb and tlyc, encoding hemolytic proteins in escherichia coli strain dh5 alpha were cloned and sequenced. the tlyb and tlyc genes, when expressed in e. coli, encode heat-labile, ... | 1994 | 7968456 |
| evaluation of microagglutination test for differentiation between serpulina (treponema) hyodysenteriae and s. innocens and serotyping of s. hyodysenteriae. | swine dysentery is a mucohemorrhagic diarrheal disease caused by the anaerobic spirochete serpulina hyodysenteriae. at present, the serotyping is done by immunodiffusion testing with lipopolysaccharide (lps) extract as antigen and rabbit hyperimmune sera produced against different serotypes of s. hyodysenteriae. since the preparation of lps is time-consuming and requires a large quantity of bacteria, it is desirable to use a serotyping method which does not require the extraction of lps. in the ... | 1994 | 7989552 |
| chemotactic response to mucin by serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization. | chemotaxis of porcine spirochetes towards a variety of mucins was measured quantitatively by a capillary method. a chemotaxis buffer consisting of 0.01 m potassium phosphate buffer (ph 7.0) and 0.2 mm l-cysteine hydrochloride was necessary for chemotaxis of spirochetes. the optimum incubation time and incubation temperature were 1 h and 40 degrees c, respectively. the mucin concentration also affected the chemotaxis observed, and a concentration of 1% (wt/vol) was near the optimum. virulent serp ... | 1994 | 8063433 |
| rapid detection of serpulina hyodysenteriae in diagnostic specimens by pcr. | a pcr assay for the detection of serpulina hyodysenteriae in diagnostic specimens was developed on the basis of sequence analysis of a recombinant clone designated pred3c6. clone pred3c6, which contained a 2.3-kb dna fragment unique to s. hyodysenteriae, was identified by screening a plasmid library of s. hyodysenteriae isolate b204 genomic dna in escherichia coli by colony immunoblot with the mouse monoclonal antibody 10g6/g10, which was produced against cell-free supernatant antigens from the ... | 1994 | 8077394 |
| physical and genetic map of the serpulina hyodysenteriae b78t chromosome. | a combined physical and genetic map of the serpulina hyodysenteriae b78t genome was constructed by using pulsed-field gel electrophoresis and dna blot hybridizations. the s. hyodysenteriae genome is a single circular chromosome about 3.2 mb in size. the physical map of the chromosome was constructed with the restriction enzymes bsshii, eclxi, noti, sali, and smai. the physical map was used to constructed a linkage map for genes encoding rrna, flagellum subunit proteins, dna gyrase, nadh oxidase, ... | 1994 | 8106320 |
| exacerbated onset of dysentery in swine vaccinated with inactivated adjuvanted serpulina hyodysenteriae. | after oral challenge exposure with serpulina hyodysenteriae-infected diced colon, fewer swine vaccinated with an experimental vaccine adjuvanted with mineral oil died (8 of 25 [32%]) than did nonvaccinated controls (6 of 15 [40%]), although the difference was not significant. however, onset and exacerbation of dysentery were accelerated in vaccinated swine because: 5 of the 8 dead vaccinated swine died before any of the nonvaccinates, which was significant (p < 0.01); vaccinated swine that died ... | 1994 | 8141499 |
| reduced virulence of serpulina hyodysenteriae hemolysin-negative mutants in pigs and their potential to protect pigs against challenge with a virulent strain. | the role of the serpulina hyodysenteriae hemolysin encoded by the tlya gene in the pathogenesis of swine dysentery (sd) was studied. tlya mutants of two s. hyodysenteriae strains (b204 and c5) were tested for virulence in pigs. none of the animals developed sd. however, after infection with wild-type strain b204 or c5, the incidence of sd was 100 or 60%, respectively. thus, the tlya-encoded hemolysin of s. hyodysenteriae is an important virulence factor in sd. the potential of these mutants to p ... | 1994 | 8188345 |
| pathogenicity of porcine intestinal spirochetes in gnotobiotic pigs. | twelve intestinal spirochete strains of porcine origin were characterized on the basis of their phenotypic properties, by multilocus enzyme electrophoresis, and by pathogenicity testing in gnotobiotic pigs. the spirochetes used included two strains of serpulina hyodysenteriae (b204 and p18a), two strains of serpulina innocens (b256 and 4/71), one strain from the proposed new genus and species "anguillina coli" (p43/6/78), and seven non-s. hyodysenteriae strains recently isolated from united king ... | 1994 | 8188364 |
| effect of divalent cations on hemolysin synthesis by serpulina (treponema) hyodysenteriae: inhibition induced by zinc and copper. | serpulina hyodysenteriae produces an oxygen-stable heat-labile hemolysin that may be an important virulence factor in the pathogenesis of swine dysentery. we examined the effect of ca2+, co2+, cu2+, fe2+, mg2+, mn2+, ni2+, and zn2+ on the hemolytic activity of cell-free supernatant (cfs) from s. hyodysenteriae, isolate b204. cells harvested from late logarithmic phase cultures were incubated in phosphate-buffered saline containing glucose and rna-core (pbs-gr) with or without cations and the hem ... | 1994 | 7801526 |
| serogroups of australian isolates of serpulina hyodysenteriae. | 1994 | 7848186 | |
| morphometric analysis of enteric lesions in c3h/hen mice inoculated with serpulina hyodysenteriae serotypes 2 and 4 with or without oral streptomycin pretreatment. | the segmental distribution and sequential progression and the role of the indigenous bacterial flora in the development of enteric lesions associated with serpulina hyodysenteriae infection in laboratory mice have not been defined. we examined the distribution and sequential morphometric changes in the large intestine of mice orally inoculated with s. hyodysenteriae serotypes 2 and 4. to determine the role of colonization resistance conferred by the indigenous bacterial flora, 40 female c3h/hen ... | 1994 | 7889460 |
| characterization of serpulina hyodysenteriae isolates of serotypes 8 and 9 from quebec by restriction endonuclease fingerprinting and ribotyping. | this study was undertaken to assess the discriminatory value of restriction endonuclease fingerprinting (ref) analysis and ribotyping of 21 serpulina hyodysenteriae isolates of serotypes 8 and 9. for ref analysis, dnas were digested with the bglii restriction enzyme and the resultant fragments were separated by polyacrylamide gel electrophoresis. for ribotyping, hybridization of bglii genomic fragments with a probe of rrnb operon using an escherichia coli rdna probe was performed on all isolates ... | 1994 | 7534207 |
| evaluation of different serological tests for detection of antibodies against serpulina hyodysenteriae in pig sera. | swine dysentery is a mucohemorrhagic diarrheal disease caused by s. hyodysenteriae. the detection of asymptomatic carriers in herds is possible by serological tests. however, cross-reactions between s. hyodysenteriae and s. innocens pose a major problem in serological diagnosis. several serological tests were evaluated for detection of antibodies to s. hyodysenteriae such as: indirect hemagglutination, passive hemolysis, conglutination and microagglutination tests. among the tests used, only the ... | 1995 | 7554822 |
| production and characterization of monoclonal antibodies specific for lipooligosaccharide of serpulina hyodysenteriae. | serpulina (treponema) hyodysenteriae is the causative agent of swine dysentery, a contagious mucohemorrhagic disease of the colon. diagnosis of swine dysentery is extremely difficult because of the presence of cross-reactive antibodies to the proteins of s. hyodysenteriae and serpulina innocens, a nonpathogenic inhabitant of the porcine large intestine. therefore, monoclonal antibodies (mabs) against the serotype-specific lipooligosaccharide (los) antigens of s. hyodysenteriae were produced to r ... | 1995 | 7559965 |
| certain canine weakly beta-hemolytic intestinal spirochetes are phenotypically and genotypically related to spirochetes associated with human and porcine intestinal spirochetosis. | four canine weakly beta-hemolytic intestinal spirochetes associated with intestinal spirochetosis (is-associated wbhis) were compared with is-associated human and porcine wbhis and the type species for serpulina hyodysenteriae and s. innocens by using phenotypic and genotypic parameters. the is-associated canine, human, and porcine wbhis belonged to a phyletic group distinct from but related to previously described serpulina type species. | 1995 | 7559984 |
| inactivation of serpulina hyodysenteriae flaa1 and flab1 periplasmic flagellar genes by electroporation-mediated allelic exchange. | serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class a and class b polypeptides. to examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the importance of motility in the etiology of this pathogen, we constructed specific periplasmic flagellar mutations in s. hyodysenteriae b204. the cloned flaa1 and flab1 genes were disrupt ... | 1995 | 7592350 |
| dna probe and polymerase chain reaction procedure for the specific detection of serpulina hyodysenteriae. | serpulina (treponema) hyodysenteriae, a gram-negative anaerobic spirochete, is the causative agent of swine dysentery, a mucohaemorrhagic diarrheal disease in which lesions are confined to the large intestine of pigs. a dna probe and polymerase chain reaction (pcr) amplification procedures which are specific, rapid , and sensitive for the detection of s.hyodysenteriae have been developed. clone pf12 from a plasmid library of s.hyodysenteriae b204 genomic dna was identified as a clone specific fo ... | 1995 | 7603470 |
| growth of serpulina (treponema) hyodysenteriae under iron-restricted conditions. | reference strains of serpulina hyodysenteriae expressed at least three iron-regulated proteins with apparent molecular masses of > 200, 134, and 109 kda when grown under iron-restricted conditions. cells of s. hyodysenteriae grown under these conditions also showed increased outer membrane bleb formation when examined by electron microscopy after negative staining. s. hyodysenteriae did not use the 2 most common types of siderophore, namely catechol and hydroxamate. western blotting with serum f ... | 1995 | 7648528 |
| the cytotoxic properties of serpulina hyodysenteriae. | examination of colonic enterocytes inoculate with pure culture of s. hyodysenteriae by phase-contrast microscopy revealed that only few spirochaetes adhere to epithelial cells. s. hyodysenteriae was observed to be highly motile, showed corkscrew-like movement which might suggest that bacteria were trying to penetrate and damaged the host cells. the pattern of motility provide evidence of a chemotaxis. supernatant of s.hyodysenteriae lysate were found to cause cte in cho, vero and pk-15 culture. ... | 1995 | 7660860 |
| expression of the smpa outer membrane lipoprotein of serpulina hyodysenteriae strain p18a in vivo. | an elisa has been developed using a monoclonal antibody (f325 ac4) to the smpa surface lipoprotein of serpulina hyodysenteriae strain p18a when grown in vitro. the lower level of detection of the elisa was approximately 5 x 10(6) spirochaetes/ml when spirochaetes were either resuspended in phosphate buffered saline or in pig faeces. when pigs were challenged with s. hyodysenteriae strain p18a the lipoprotein was detected in the faeces of pigs by elisa when the numbers of spirochaetes excreted wa ... | 1995 | 7667904 |
| serpulina hyodysenteriae challenge of fattening pigs vaccinated with anadjuvanted bivalent bacterin against swine dysentery. | an experimental fattening period was performed with 36 pigs in order to evaluate the safety and efficacy of a bivalent adjuvanted serpulina hyodysenteriae bacterin. the pigs originated from three different farms and were split into six groups. eighteen pigs were vaccinated twice, two weeks apart and the rest served as controls. one unvaccinated pig in each group was inoculated intragastrically with 4 x 10(8) active s. hyodysenteriae spirochaetes, b 78 strain. weight evolution, clinical symptoms, ... | 1995 | 7668035 |
| susceptibility of porcine ileal enterocytes to the cytotoxin of serpulina hyodysenteriae and the resolution of the epithelial lesions: an electron microscopic study. | the cytotoxin from serpulina hyodysenteriae was injected into ileal loops of eight germ-free pigs, and the effects on the villi were observed after 1, 3, and 18 hours of exposure. the mature vacuolated villus enterocytes of the proximal part of the absorptive villi were most susceptible to the lethal effects of the cytotoxin and were extensively exfoliated. the enterocytes at the base of the villi, the goblet cells, and the follicle-associated epithelium of the dome villi, particularly the m cel ... | 1995 | 7725595 |
| comparative study of the enzyme activities of borrelia burgdorferi and other non-intestinal and intestinal spirochaetes. | comparative analysis of the enzymatic profiles of 58 spirochaetal isolates clearly differentiated borrelias from leptospires, serpulinas and a treponeme. strains of both borrelia burgdorferi and borrelia hermsii characteristically produced significant amounts of leucine arylamidase. this enzyme activity was not unique to borrelias but was also detected amongst pathogenic and non-pathogenic leptospira serovars. this fact, however, did not hamper a correct differentiation of borrelias from these s ... | 1995 | 7760753 |
| prophylactic effect of dietary zinc in a laboratory mouse model of swine dysentery. | reduced prevalence of diarrhea and mortality has been reported after dietary supplementation with zinc compounds in swine with naturally acquired colibacillosis and those challenge-exposed with serpulina hyodysenteriae; however, the usefulness of this approach for control of enteric diseases of swine remains to be determined. to examine the effect of dietary zinc-containing compounds on the colonization and development of cecal lesions associated with s hyodysenteriae infection, a defined diet a ... | 1995 | 7771701 |
| use of commercial enzyme kits and fatty acid production for the identification of serpulina hyodysenteriae: a potential misdiagnosis. | the accuracy of identification of serpulina hyodysenteriae strains grown in a complex medium was 90% when 2 commercial test kits were used. unlike the other s. hyodysenteriae strains, s. hyodysenteriae strain p35/2 was unusual in being indole negative. the nonpathogenic intestinal spirochete pws/a, which is from a different species, was indole positive and alpha-galactosidase negative. identification of these spirochetes on the basis of these kits alone would have been incorrect. the analysis of ... | 1995 | 7779972 |
| survival of serpulina hyodysenteriae in an effluent lagoon. | to determine the survival of serpulina hyodysenteriae in an infected lagoon that received effluent from a confinement building housing swine dysentery-infected swine. | 1995 | 7493879 |
| production and characterization of monoclonal antibodies against serpulina hyodysenteriae and s. innocens and their use in serotyping. | murine monoclonal antibodies (mabs) directed against serotypes 1, 2, 8, and 9 of serpulina hyodysenteriae and strain b256 of serpulina innocens were produced and characterized. a serological classification of 96 field strains of s. hyodysenteriae and 28 field strains of s. innocens isolated from pigs showing clinical signs of swine dysentery was performed by rapid dot enzyme-linked immunosorbent assay (elisa) with the mabs. the results indicated that the majority of the field strains of s. hyody ... | 1995 | 7494066 |
| distribution of the smpa gene from serpulina hyodysenteriae among intestinal spirochaetes. | forty intestinal spirochaete strains were investigated for nucleotide sequences related to the smpa locus from serpulina hyodysenteriae by southern hybridization of chromosomal dna using the smpa locus from s. hyodysenteriae strain p18a as a probe and by pcr using primers internal to the smpa gene. the intensity of the hybridization signal at high stringency and positive pcr results suggested that 12 s. hyodysenteriae strains possessed a similar nucleotide sequence. pcr was negative for another ... | 1995 | 7496514 |
| isolation of extracytoplasmic proteins from serpulina hyodysenteriae b204 and molecular cloning of the flab1 gene encoding a 38-kilodalton flagellar protein. | extracytoplasmic proteins were released from serpulina (treponema) hyodysenteriae (strain b204) by treatment of whole cells with a nonionic detergent (tween 20). centrifugation of the tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, 42, 39, 38, 34, 33.5, 33, 31, and 29 kda. treatment of the sedimented fraction with 6 m urea solubilized all of the proteins except the 39-kda protein. peptide sequences were obtain ... | 1995 | 7806350 |
| identification of a new intestinal spirochete with pathogenicity for chickens. | two intestinal spirochete isolates obtained from chickens with diarrhea were examined by electron microscopy, biochemical tests, rrna gene restriction pattern analysis, and multilocus enzyme electrophoresis. one isolate (strain 91-1207/c1) was pathogenicity tested in vivo in chickens. the chicken spirochetes were morphologically indistinguishable from serpulina innocens and serpulina hyodysenteriae and phenotypically similar to s. innocens. however, the chicken spirochetes could be distinguished ... | 1995 | 7822007 |
| phenotypical characterisation of intestinal spirochaetes isolated from pigs. | a combined evaluation of the phenotypical properties of five serpulina type or reference strains and 163 swedish isolates of spirochaetes from pigs and two from birds was made. the porcine isolates were collected from herds with a history of dysentery or severe diarrhoea and from herds chosen at random. on the basis of beta-haemolysis, indole production, hippurate hydrolysis, and alpha-galactosidase, alpha-glucosidase and beta-glucosidase activity, the isolates could be divided into four main gr ... | 1995 | 8525077 |
| swine dysentery: more unknown than known. | swine dysentery (sd) is an economically important disease. it is caused by the spirochete serpulina hyodysenteriae. in order to minimize the economic damage of sd, several methods to control this disease are recommended. whereas hygienic measures and use of antimicrobials are actually practised for prevention, detection and exclusion of carriers of s. hyodysenteriae and vaccination against the disease still needs further attention. the last two methods require reliable and sensitive diagnostic t ... | 1995 | 8560732 |
| mitomycin c induction of bacteriophages from serpulina hyodysenteriae and serpulina innocens. | a prophage was induced from cells of the pathogenic spirochaete serpulina hyodysenteriae using mitomycin c. five to seven hours after mitomycin c was added (8 micrograms/ml, final concentration) to s. hyodysenteriae b204 cultures in bhis broth (od620 = 0.9) cell lysis was detected as a decrease in culture optical density. bacteriophage particles attached to whole cells and to cell debris were detected by electron microscopic analysis of negatively stained (2% pta, ph 7.0) bacteria harvested by c ... | 1995 | 8593962 |
| pigs experimentally infected with serpulina hyodysenteriae can be protected from developing swine dysentery by feeding them a highly digestible diet. | weaner pigs (n = 72) were fed 1 of 4 diets. these were based on either cooked rice and animal protein, cooked rice and lupin, wheat and lupin, or wheat and animal protein. twenty-six of the pigs were slaughtered after 1 month. those fed the highly digestible cooked rice and animal protein diet had drier colonic contents and faeces, lighter large intestines, and the contents of their large intestines had increased ph values and decreased total vfa concentrations. the other 46 were orally challeng ... | 1996 | 8620913 |
| sensitivity of strains of serpulina hyodysenteriae isolated in hungary to chemotherapeutic drugs. | the sensitivity of 332 strains of serpulina hyodysenteriae isolated in hungary between 1978 and 1992 was tested against seven chemotherapeutic drugs frequently used for the treatment of swine dysentery, and the changes in the patterns of resistance were also monitored. all the strains remained sensitive to carbadox, with minimum inhibitory concentrations (mic) of only 0.05 to 0.40 microgram/ml at present. the susceptibility of the strains to dimetridazole has gradually decreased, but about half ... | 1996 | 8677605 |
| identification and characterization of genus-specific epitopes of serpulina species using monoclonal antibodies. | four murine monoclonal antibodies (mabs) designated as c9e8, a10, g12, and g8 which recognized both serpulina hyodysenteriae and s. innocens were produced and characterized. the mabs reacted with whole cell antigens in elisa, indirect immunofluorescence and immunoblot assays. the mabs did not show any cross reactivity in rapid dot elisa or immunoblot assay with leptospira icterohemorrhagiae, campylobacter jejuni and escherichia coli. treatment of whole cell suspension with proteinase k and sodiu ... | 1996 | 8701579 |
| sensitivity of serpulina hyodysenteriae to tiamulin in the uk. | 1996 | 8711890 | |
| cell membrane permeability and mitochondrial dysfunction-inducing activities in cell-free supernatants from serpulina hyodysenteriae serotypes 1 and 2. | membrane permeability (mp) and mitochondrial dysfunction-inducing (mdi) activities were detected in cell-free supernatants (cfs) of serpulina hyodysenteriae, using either hemoglobin release from porcine red blood cells (rbc) or cytoplasmic lactate dehydrogenase release from porcine peripheral blood lymphocytes (pbl), and reduction of the 3-(4,5-dimethylthiazoyl-2-y1)-2,5-diphenyltetrazolium bromide dye by porcine pbl. the mp and mdi activities of cfs correlated with each other for serotype 1 and ... | 1996 | 8800549 |
| development of an experimental model allowing discrimination between virulent and avirulent isolates of serpulina (treponema) hyodysenteriae. | variation in virulence among different strains of serpulina hyodysenteriae was studied by oral inoculation of specific pathogen free piglets and cd-1 mice. piglets infected with serotype 2 reference strain b204 and an untypable field strain lhv-90-9-i had severe diarrhea tainted intermittently with mucus and fresh blood. the piglets inoculated with b169, b8044, b6933, and ack300-8 reference strains representing serotypes 3, 5, 6, and 7 respectively developed moderate diarrhea. however, reference ... | 1996 | 8825993 |
| motility and chemotaxis in serpulina hyodysenteriae. | chemotactic- or motility-regulated mucus association appears to be the predominant mechanism of mucosal association by the causative agent of swine dysentery, serpulina hyodysenteriae. in the present study, a modification of the adler capillary assay was used to evaluate the chemotactic responses of s. hyodysenteriae to a variety of potential stimuli. first, however, it became necessary to study factors that influenced motility of the spirochete in vitro, since standard cultivation methods produ ... | 1996 | 8861640 |
| differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16s rrna sequence comparisons. | multilocus enzyme electrophoresis (mee) analysis and comparisons of nearly complete 16s rrna gene sequences (1416 nucleotide positions) were used to evaluate phylogenetic relationships among serpulina hyodysenteriae strain b78t, s. innocens strain b256t, brachyspira aalborgi strain 513at, and eight uncharacterised strains of swine, avian, and human intestinal spirochaetes. from mee analysis, nine strains could be assigned to five groups containing other intestinal spirochaetes (genetic distances ... | 1996 | 8869502 |
| production and characterisation of a monoclonal antibody to serpulina hyodysenteriae. | a monoclonal antibody (mab) directed against serpulina hyodysenteriae, the causative agent of swine dysentery, was produced and characterised. the mab (bjl/sh1) reacted in western blots with a protein with a molecular mass of about 30 kda in outer membrane preparations from a range of s. hyodysenteriae isolates of different serotypes. it did not react with preparations made from a variety of non-s. hyodysenteriae intestinal spirochaetes. immunogold labelling was used to confirm the location of t ... | 1996 | 8869504 |
| the incidence of swine dysentery in pigs can be reduced by feeding diets that limit the amount of fermentable substrate entering the large intestine. | two experiments were conducted to test the hypothesis that feeding diets which limit the amount of fermentable substrate entering the large intestine would protect pigs against experimental infection with serpulina hyodysenteriae, the causative agent of swine dysentery. experiment 1 examined the effect of grain processing (hammer milling vs. steam flaking) and grain type (barley, groats, corn, sorghum and wheat) on indices of fermentation in the large intestine and the incidence of swine dysente ... | 1996 | 8914966 |
| dual flaa1 flab1 mutant of serpulina hyodysenteriae expressing periplasmic flagella is severely attenuated in a murine model of swine dysentery. | the motility imparted by the periplasmic flagella (pf) of serpulina hyodysenteriae is thought to play a pivotal role in the enteropathogenicity of this spirochete. the complex pf are composed of multiple class a and class b polypeptides. isogenic strains containing specifically disrupted flaal or flab1 alleles remain capable of expressing pf, although such mutants display aberrant motility in vitro. to further examine the role that these proteins play in the maintenance of periplasmic flagellar ... | 1996 | 8926083 |