Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [helicobacter pylori associated gastritis: a primary study of ammonia and urea in gastric juice and mucus in gastric mucosa]. | the mucosa of 46 patients with histological chronic gastritis were cultured for helicobacter pylori (hp) and the ammonia and urea in their gastric juice were also determined. the results showed the average ammonia concentration (1.22 +/- 0.23 mmol/l) in hp positive patients was higher than that of hp negative patients (0.72 +/- 0.25 mmol/l), (p < 0.05). but the average urea concentration (0.45 +/- 0.29 mmol/l) in hp positive patients was lower than that of hp negative patients (2.71 +/- 1.20 mmo ... | 1993 | 8244308 |
| helicobacter pylori infection in japanese patients with adenocarcinoma of the stomach. | to examine the association of helicobacter pylori infection with adenocarcinoma of the stomach in japanese patients, we studied 29 patients and 58 matched controls. ascertainment of h. pylori status was based on the presence of specific igg to h. pylori. for the entire group, an association of this infection with gastric adenocarcinoma was suggested but not statistically significant. for patients in a putatively high-risk subgroup (non-cardia tumors and age < or = 70 years), the association was ... | 1993 | 8244577 |
| direct dna amplification and restriction pattern analysis of helicobacter pylori in patients with duodenal ulcer and their families. | sera from 14 patients with duodenal ulcer and their families were tested for igg antibodies to helicobacter pylori. fourteen serologically negative patients and their families served as controls. index patients and their family members who were serologically positive were advised to undergo endoscopic biopsy. gastric biopsy tissues were subjected to haeiii restriction analysis of nested polymerase chain reaction products of the urease gene. by serology, 28 (49.0%) of 57 in index families and 11 ... | 1993 | 8245543 |
| diagnosis of helicobacter pylori infection in a developing country: comparison of two elisas and a seroprevalence study. | serology to detect antibodies to helicobacter pylori is not frequently used as a diagnostic tool in developing countries. when compared to a commercial elisa, an elisa constructed and validated in thailand had a higher sensitivity (98% vs. 85%), specificity (76% vs. 66%), and negative predictive value (97% vs. 76%) for the detection of h. pylori infection among 104 patients with dyspepsia evaluated by endoscopy. the positive predictive value was 88% for both tests. serum antibody levels fell sig ... | 1993 | 8245544 |
| [new development on the study of etiological pathology of gastritis, peptic ulcer and stomach cancers in relationship to helicobacter pylori infection]. | 1993 | 8245642 | |
| determination of the optimal transport system for helicobacter pylori cultures. | a range of solid and liquid media was evaluated for the ability to maintain survival of helicobacter pylori strains under different conditions. chocolate agar slopes maintained survival of most strains for longer than 3 days, some strains surviving for up to 9 days, despite a decreased number of viable cells. temperature and atmosphere did not significantly influence the performance of these slopes. the bbl campy pouch system also achieved a considerable recovery rate of h. pylori after storage ... | 1993 | 8246249 |
| evaluation of the polymerase chain reaction for detecting the urease c gene of helicobacter pylori in gastric biopsy samples and dental plaque. | a polymerase chain reaction (pcr) assay with oligonucleotide primers homologous to a portion of the urease c gene of helicobacter pylori was evaluated for specificity with pure dna and biopsy material. the assay was used to test for the presence of the organism in dental plaque. the species specificity of detection was confirmed by ensuring that the primers did not amplify dna extracts from h. cinaedi, h. felis, h. fennelliae, h. mustelae and h. nemestrinae. sixty-two gastric biopsy samples coll ... | 1993 | 8246250 |
| [should helicobacter pylori infections be treated?]. | 1993 | 8247715 | |
| gastritis associated with gastrospirillum hominis in children. comparison with helicobacter pylori and review of the literature. | interest in possible microbiological causes of gastritis has significantly increased since the discovery of helicobacter pylori. recently a spiral bacterium named gastrospirillum hominis was described in association with chronic gastritis in adult patients. here, we present the finding of gastrospirillum hominis in the gastric biopsies of two children who underwent upper endoscopy for gastrointestinal symptoms. the frequency of gastrospirillum hominis (0.3%) in our pediatric population was simil ... | 1993 | 8248105 |
| an evaluation of factors affecting helicobacter pylori prevalence in tibetans exiled in india. | helicobacter pylori infection is found world-wide although the epidemiology of infection has not been well defined in many geographical areas. the aims of this study were to determine the prevalence of h. pylori infection and chronic gastritis and the demographic correlates of infection in a single racial group in southern india. the sample population was selected randomly from the male population register of a tibetan refugee settlement. demographic data and results of endoscopy with antral muc ... | 1993 | 8249063 |
| [incidence of helicobacter pylori infections in patients with duodenogastric reflux]. | the purpose of the work was a comparison of the incidence of helicobacter pylori (hp) infections in persons with dyspeptic symptoms of non-ulcer dyspepsia (nud) character in relation to the presence or absence of duodenogastric reflux (dgr). the study included 80 patients aged from 17 to 80 years, 21 males and 59 females. in all studied patients endoscopy of upper digestive tract segment was performed with biopsy specimen taking for microbiological investigations. from each patient two biopsy sp ... | 1993 | 8249413 |
| [gastric hypersecretion, helicobacter pylori, and duodenal ulcer]. | 1993 | 8249528 | |
| acid secretion and serum gastrin in normal subjects and patients with duodenal ulcer: the role of helicobacter pylori. | to compare gastric secretory function in patients with duodenal ulcer and in healthy volunteers with and without helicobacter pylori infection. | 1993 | 8249971 |
| [helicobacter pylori infection and peptic ulcer]. | 1993 | 8253034 | |
| [helicobacter pylori infection and peptic ulcer]. | 1993 | 8253035 | |
| [follicular gastritis in adults. relations with helicobacter pylori, histological and endoscopic aspects]. | follicular gastritis (fg) is characterized by lymphoid follicle hyperplasia in the gastric mucosa. the aim of this prospective study was to determine the prevalence of fg in adults, their relation to helicobacter pylori infection, and their histological and endoscopic features. of 445 patients (379 men, 66 women), 36.4 years old (range: 18-86), fg was detected in 63 patients (14.2%). this was highly significantly associated with h. pylori infection: 49/138 infected patients (35.5%) versus 14/307 ... | 1993 | 8253308 |
| demonstration of a phospholipid-rich zone in the human gastric epithelium damaged by helicobacter pylori. | recently, a hydrophobic layer has been shown by the contact angle method in human gastric biopsy specimens. the aim of this study was to show the existence of a phospholipid-rich layer in the human gastric epithelium and to investigate the influence of helicobacter pylori on these structures. | 1993 | 8253346 |
| gastric ammonia has a potent ulcerogenic action on the rat stomach. | the pathophysiological mechanism by which helicobacter pylori induces mucosal injury has not been clarified. the aim of this study was to investigate the role of urea, urease, and ammonia in rat gastric mucosal lesions using an ex vivo chamber model. | 1993 | 8253347 |
| comparison of commercial diagnostic tests for helicobacter pylori antibodies. | a number of serological tests measuring the presence of helicobacter pylori-specific serum immunoglobulin g (igg) are now commercially available. the aim of this study was to evaluate the clinical accuracy of five commercial h. pylori antibody tests: gap-igg (biomerica), helptest (amrad, kew, victoria, australia), helico-g (porton cambridge), pyloriset (orion diagnostica), and roche (roche diagnostics). a total of 162 subjects presenting for routine upper endoscopy were studied. h. pylori was di ... | 1993 | 8253958 |
| isolation of helicobacter pylori from saliva. | helicobacter pylori was grown in low numbers from the saliva of one of nine patients who were positive for gastric h. pylori. the saliva-derived isolate from this patient was identical to the antral biopsy-derived isolate from the same patient and differed from isolates cultured from the antral biopsies of all other patients by soluble-protein electrophoresis, restriction endonuclease dna analysis, and southern blot hybridization. this is the first observation, to our knowledge, of the recovery ... | 1993 | 8253990 |
| gastric cancer and helicobacter pylori infection. | to identify differences in the prevalence of helicobacter pylori infection in different groups of patients with gastric cancer. | 1993 | 8254106 |
| transformation of helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. | most strains of helicobacter pylori are naturally competent for uptake of chromosomal dna. transformation frequencies for streptomycin resistance or rifampicin resistance markers ranged from 1 x 10(-4) to 1 x 10(-3) per viable cell using a plate transformation procedure. transformation of a metronidazole resistance marker (mtrr) was demonstrated when either a laboratory-derived mutant or a mtrr clinical isolate were used as the source of donor dna. mtrr was transformed at a frequency of 3 x 10(- ... | 1993 | 8254319 |
| use of endoscopic biopsies and microelectrodes to study human gastric acid secretion in vitro. | we have investigated the possibility of measuring acid secretion from human gastric mucosa in vitro as a potential pharmacological preparation. we used open-ended 10 microns-tip, lix-based glass microelectrodes to measure the ph of the mucus layer of gastric biopsies superfused with a hepes buffered solution in an organ bath. with no drugs added the ph of the mucus layer of biopsies from the body of the stomach stayed constant but the ph of antral biopsies fell slightly by a median of 0.12 ph un ... | 1993 | 8255402 |
| chronic gastritis, prostaglandins and helicobacter pylori. | 1993 | 8256482 | |
| [histological lesions and helicobacter pylori colonization in children]. | we have assessed the relationship between histologic findings and the helicobacter pylori (hp) colonization in 115 patients (age: 9.8 +/- 3.5 years) with hp infection. normal antral histology was found in 8.7% of the patients. signs of histological activity were observed in 58.2% of the patients and glandular lesions in 3.5%. a significantly greater hp colonization was observed in patients with signs of histological activity (p < 0.001). a significant correlation was found between the hp coloniz ... | 1993 | 8256947 |
| pepsinogens in health and disease. | pepsinogens, precursors of pepsins (potent and abundant digestive enzymes that are the primary products of the gastric chief cells), are members of the family of aspartic proteases. because of the heterogeneity of pepsinogens, several classifications have appeared in the literature. i describe the recommended classification and nomenclature of the aspartic proteases and discuss their genetics, biochemistry (structure, activation of zymogens, mechanism of proteolytic activity and inhibitors), and ... | 1993 | 8260073 |
| helicobacter pylori prevalence in a routine upper gastrointestinal endoscopy population. | the presence of helicobacter pylori (hp) in gastric biopsy specimens of 500 patients referred for routine upper gastrointestinal endoscopy for various abdominal complaints was investigated histologically and microbiologically. hp was detected in 429 of the 500 patients (86%). antral biopsy specimens revealed gastritis in 457 out of 500 cases (91.4%). in the 43 patients who had normal histological findings, only 3 had hp infection (7%). the prevalence of hp in the patients with gastric and duoden ... | 1993 | 8260335 |
| non-ulcer dyspepsia and gastritis--clinical aspects. | although patients with non-ulcer dyspepsia (nud) and peptic ulcer disease present similar symptoms, the two diseases are pathophysiologically completely different and should be treated accordingly. the diagnosis of nud is still based on negative criteria, i.e., organic diseases must be excluded. the majority of the patients with nud have a functional disorder possibly as a consequence of psychological stress which, by activating processes within the central nervous system, may evoke a sequence o ... | 1993 | 8260734 |
| intragastric distribution and gastric emptying of solids and liquids in functional dyspepsia. lack of influence of symptom subgroups and h. pylori-associated gastritis. | the relative contributions of altered gastric motor function and helicobacter pylori-associated active chronic gastritis to the pathogenesis of functional dyspepsia are controversial. we therefore evaluated scintigraphically the intragastric distribution and gastric emptying of a mixed solid-liquid meal in 75 patients with functional dyspepsia; patients were subdivided on the basis of both specific symptom clusters and the presence or absence of h. pylori gastritis. twenty-one (28%) patients dis ... | 1993 | 8261829 |
| different interdigestive antroduodenal motility patterns in chronic antral gastritis with and without helicobacter pylori infection. | fasting antroduodenal motor activity was studied in 15 dyspeptic patients with chronic superficial antral gastritis and helicobacter pylori infection (group a), 10 dyspeptic patients with chronic superficial antral gastritis without helicobacter pylori infection (group b), and eight healthy control subjects (group c) by manometric recording of phases of the interdigestive migrating motor complex (mmc) prolonged over 240 min. a significantly lower incidence of activity fronts (phase iii of mmc) s ... | 1993 | 8261830 |
| role of helicobacter pylori serology in evaluating treatment success. | duodenal ulcer recurrence and gastritis are reduced with successful helicobacter pylori treatment. serology is accurate in the diagnosis of h. pylori, but its value in determining eradication is unproved. to evaluate the usefulness of serology in monitoring treatment, we measured serial serum antibodies in three patient groups: eradication success (n = 57), eradication failure (n = 19), and untreated patients (n = 24). eradication was determined by warthin starry staining of antral biopsies and ... | 1993 | 8261831 |
| helicobacter pylori in gastroduodenal diseases: rapid identification by endoscopic brush cytology. | previous reports showed helicobacter pylori (h. pylori) in type b gastritis-affected stomachs. this study was carried out to compare h. pylori staining effectiveness on biopsy to brush cytology. tissue and brush parallel samples of gastric mucosa with abnormal or normal appearances were examined: 57.6% h. pylori-positive pieces from the antrum and 19.2% from the body were found, versus 65.3% and 25% h. pylori-positive brush smears, respectively. h. pylori resembling organisms were mainly related ... | 1993 | 8261849 |
| cell vacuolization induced by helicobacter pylori: inhibition by bafilomycins a1, b1, c1 and d. | all available bafilomycins (a1, b1, c1 and d) inhibit and revert macroscopic vacuolization induced by helicobacter pylori cell-free extracts. bafilomycin a1 displays the highest activity, followed by bafilomycin b1, c1 and d. the different potency of bafilomycins correlates with their ability to inhibit the vacuolar-type atpase (v-atpase) and to dissipate the membrane ph gradient of intracellular acidic organelles. these results suggest that bafilomycins should be considered as possible therapeu ... | 1993 | 8262366 |
| [drug resistance of helicobacter pylori strains isolated from patients with inflammation of stomach mucous membrane and peptic ulcer of stomach and duodenum]. | twelve strains of h. pylori were tested. they were isolated from biosamples from 23 patients with stomach inflammation (16 persons) and stomach (3) and duodenum peptic ulcer. by application of solid medium dilution of mic50 and mic90-16 antibiotics and antimicrobials were tested. values of mic50 and mic90 for antibiotics from the tetracycline group were, respectively 0.25-0.5 microgram/ml and 0.12-1.0 microgram/ml. similar results were obtained with macrolide antibiotics. all strains were sensit ... | 1993 | 8189807 |
| [helicobacter pylori in mucous membrane of stomach and duodenum of patients with symptoms of ulceration and dyspepsia]. | the material consisted of samples of mucous membrane of stomach and duodenum obtained during endoscopy in patients with clinical symptoms of peptic ulcer of stomach or the duodenum or with dyspeptic problems. samples were tested for presence of h. pylori by culture on brain-heart agar supplemented with 7% of horse blood. direct test for urease production was also performed. isolated strains were identified basing on morphology of growth, gram-stained preparation, mobility of microorganism, produ ... | 1993 | 8189827 |
| metronidazole uptake by sensitive and resistant isolates of helicobacter pylori. | the development of metronidazole resistance in helicobacter pylori has become an important factor in the clinical use of this drug in the treatment of gastritis and peptic ulcers. the mechanism for the development of resistance is not known but may involve factors which influence metronidazole uptake and metabolism. in this study, a simple and reliable hplc assay for metronidazole was developed and the uptake of the drug from broth cultures of both resistant and sensitive strains of h. pylori wa ... | 1993 | 8262861 |
| a simple and efficient continuous culture system for helicobacter pylori. | helicobacter pylori has been successfully maintained in a continuous culture system. the culture was grown in 0.6 litre of brain heart infusion broth with supplements in a 1 litre fermenter with a flow rate of 25 ml/h, an 80% dilution rate and a specific growth rate of 0.06/h. unlike the typical growth curve which occurred in batch culture, the growth pattern in continuous culture showed a short lag phase following which growth was maintained in exponential phase with > or = 10-fold increase in ... | 1993 | 8264435 |
| [evaluation of the elisa method for the diagnosis of helicobacter pylori]. | antibodies to h.pylori were investigated in the serum of 50 patients with dyspepsia and 25 healthy controls with elisa method. the antibody positivity was found in all of the 40 cases (100%) for whom h.pylori was demonstrated in at least two tests of rapid urease, gram stain, culture and silver staining and in eight of the ten cases (80%) whom h.pylori tests were all negative. twenty-two of the 25 healthy controls (88%) had also antibodies to h.pylori. the sensitivity of the test was found high ... | 1993 | 8264442 |
| helicobacter pylori eradication: efficacy and side effect profile of a combination of omeprazole, amoxycillin and metronidazole compared with four alternative regimens. | we evaluated eradication of helicobacter pylori infection in 263 patients by a new 14-day regimen of omeprazole 40 mg mane (a gastric secretory inhibitor) plus two antibiotics: amoxycillin 500 mg three-times daily (tds) plus metronidazole 400 mg tds. the comparative groups included updated results of our previous work with a 14-day course of either standard triple therapy (stt, colloidal bismuth subcitrate 120 mg four times daily (qds) plus tetracycline 500 mg qds and metronidazole 400 mg tds), ... | 1993 | 8265776 |
| an in vitro study of enhanced h+ diffusion by urease action on urea. implications for helicobacter pylori-associated peptic ulceration. | the in vitro effect of urea and hydrolysis of urea by urease on mucus h+ permeability is reported here. the effective dhcl values indicate a strong ph dependence for h+ diffusion in both water and mucus layers, with no apparent trend at concentrations between 1 and 50 mm urea. however, the estimated dhcl at near-neutral and alkaline ph are 4- to 10-fold lower through mucus than through aqueous films. moreover, the pka values of hco3- and nh3 (generated by urease action on urea) had a profound ef ... | 1993 | 8266022 |
| intestinal metaplasia, not atrophy or achlorhydria, creates a hostile environment for helicobacter pylori. | a 54-year-old man with dyspepsia, helicobacter pylori-associated chronic active atrophic gastritis without intestinal metaplasia, and hyperplastic gastric polyps was followed up for 1 year after h. pylori eradication with sequential endoscopic gastric mucosal mapping and gastric function tests. eradication of h. pylori by triple therapy resulted in the histologic resolution of gastritis. however, the patient's condition, gastric function, and morphology of the polyps were not affected. this case ... | 1993 | 8266024 |
| evaluation of therapies in the treatment of helicobacter pylori associated non-ulcer dyspepsia. | 1993 | 8266168 | |
| [hemoptysis as manifestation of a helicobacter pylori infection]. | a 14-year-old girl was transferred to our unit after 6 weeks of repeating episodes of what was thought to be 'hemoptysis'. apart from discrete nausea, she did not have any other complaints. the episodes of 'hemoptysis' occurred once every 3 days, mostly in the late evening. the physical examination was strictly normal. a thorough investigation revealed a chronic active gastritis and helicobacter pylori infection. no lung disease could be confirmed. treatment consisted of a combination therapy wi ... | 1993 | 8266314 |
| [mechanisms in hypergastrinemia in autoimmune atrophic gastritis and helicobacter pylori infection]. | the authors review recent progresses made in the understanding of the disturbed gastrin homeostasis in helicobacter pylori infection and in pernicious anaemia. regulation of gastrin release in a complex mechanism involving inhibition by a low gastric ph and several peptides including somatostatin, and stimulation by different factors, mainly alimentary peptides and amino-acids. the hypergastrinaemia observed in patients with helicobacter pylori infection occurs despite a normal intraluminal ph. ... | 1993 | 8266764 |
| the role of helicobacter pylori in acid-peptic disease. | acid peptic disease is common, and its management is costly. less than a decade ago, the traditional theories regarding the etiology and pathogenesis of acid peptic disease were upset by the discovery of helicobacter pylori infection in association with chronic active gastritis. a substantial body of investigation after that discovery has established this infection as the major cause of human chronic active gastritis and has defined a critical role for h. pylori in the etiology, pathophysiology, ... | 1993 | 8266980 |
| helicobacter pylori: a decade later. | 1993 | 8266981 | |
| evaluation of eight commercial kits for helicobacter pylori igg antibody detection. | eight commercial kits and an in-house elisa for detection of igg antibodies against helicobacter pylori were evaluated for their use in diagnosis of h. pylori infection and in epidemiological research: helico-gtm (porton-cambridge), g. a. p. test (bio-rad), h. pylori antibodies elisa (biometra), anti-h. pylori igg eia (roche), 2nd generation h. pylori eia (roche), anti-h. pylori mtp-assay (roche), pylori stat test kit (whittaker), pyloriset latex agglutination kit (orion), and the in-house elisa ... | 1993 | 8267957 |
| antral helicobacter pylori infection, hypergastrinemia and peptic ulcers: effect of eradicating the organism. | a randomized prospective study on the response of fasting serum gastrin concentrations in peptic ulcer patients was performed in order to test the hypothesis that h. pylori infection in the gastric antrum increases gastrin release, and to examine whether the high fasting serum gastrin concentrations respond to treatment that eradicates h. pylori. | 1993 | 8268142 |
| comparison of the urease test and of direct smear examination in the control of treatment of helicobacter pylori-induced infection. | the sensitivity and specificity of the preformed urease test and of carbolfuchsin-stained smears for the diagnosis of the presence of helicobacter pylori in gastric mucosa were evaluated before and after antimicrobial treatment. the results obtained by culture were used as the reference point. we studied 41 patients with endoscopically diagnosed duodenal ulcer. twenty-five of these were treated with furazolidone (100 mg t.i.d.), amoxicillin (500 mg t.i.d.) and metronidazole (250 mg t.i.d.) for 5 ... | 1993 | 8268818 |
| [an urease enzyme linked immunosorbent assay for detection of helicobacter pylori infection]. | a sensitive and specific serological diagnostic test for helicobacter pylori infection has been developed and validated in 120 patients with dyspeptic symptoms undergoing endoscopy. this test is to use urease, a protein unique to h. pylori, as the basis for the enzyme linked immunosorbent assay (elisa) that detects serum h. pylori urease antibodies. the elisa mean optical density (od) in h. pylori-positive group is higher than that in h. pylori-negative group (0.57 +/- 0.23 vs 0.24 +/- 0.15, p < ... | 1993 | 8269756 |
| the coccoid forms of helicobacter pylori. criteria for their viability. | the fact that helicobacter pylori can revert to a coccoid form has stimulated speculation about its role in transmission and as a possible cause of reinfection in duodenal ulcer disease. bismuth subcitrate (32 micrograms/ml), bismuth subsalicylate (64 micrograms/ml), amoxicillin (0.05 micrograms/ml) and erythromycin (4 micrograms/ml) inhibited the growth of h. pylori and stimulated the formation of basically respiring but non-culturable coccoid structures. the presence of polyphosphates as energ ... | 1993 | 8270008 |
| [quantification of helicobacter pylori in the gastric mucus by competitive pcr using synthetic dna fragments]. | 1993 | 8271474 | |
| [does no helicobacter pylori mean no duodenal ulcer?]. | 1993 | 8272133 | |
| chronic gastritis associated with infection due to helicobacter pylori in southern appalachian veterans with dyspepsia. | a prospective study of infection due to helicobacter pylori in 104 southern appalachian veterans with upper gastrointestinal symptoms revealed a prevalence of 67%. there was no age difference observed between those with and without h pylori. the organism was shown to be present in 12 of 13 patients with duodenal ulcer (92%), 6 of 10 with gastric ulcer (60%), and 52 of 81 with nonulcer dyspepsia (64%). using culture as standard, the urease test showed a sensitivity of 76% and specificity of 100%, ... | 1993 | 8272911 |
| fumarate catabolism in helicobacter pylori. | the metabolism of fumarate by helicobacter pylori was investigated employing one- and two-dimensional 1h and 13c nuclear magnetic resonance spectroscopy. metabolically competent cells generated malate and succinate from fumarate as the sole substrate indicating the presence of fumarase and fumarate reductase activities in the bacterium. in incubations of fumarate with cell lysates accumulation of lactate, acetate, formate and alanine was observed after the initial production of malate and succin ... | 1993 | 8275020 |
| helicobacter pylori and peptic ulcer disease. | medical therapy for duodenal or gastric ulcer disease has traditionally involved gastric acid antisecretory therapy for 4 to 8 weeks to promote initial healing and indefinitely to prevent recurrences of ulcer. the discovery of helicobacter pylori in most patients with peptic ulcer disease has led to a change in this approach. therapy designed to eradicate h pylori may facilitate ulcer healing with acid antisecretory agents and, more important, may greatly reduce the incidence of ulcer recurrence ... | 1993 | 8279151 |
| non-cardia gastric adenocarcinoma and helicobacter pylori infection. | the seroprevalence of igg antibodies to helicobacter pylori (hp-ab) was studied in 47 patients (29m, 18f, mean age +/- sd: 62.44 +/- 12.63 years) with non-cardia gastric carcinoma using an enzyme-linked immunosorbent assay. controls were: a) 50 healthy people well-matched with the cancer patients; b) 50 patients with dyspepsia well matched with the cancer patients. hp-ab were detected in 72.3% of patients with gastric carcinoma, in 68% of healthy people and in 88% of dyspeptics. no significant a ... | 1993 | 8280897 |
| special issue. the microbiology of helicobacter pylori. assessment of recent experimental evidence on the pathophysiology, cancerogenic potential, immunity and chemotherapy of h. pylori infections. | 1993 | 8280929 | |
| adherence of haemagglutinating helicobacter pylori to five cell lines. | helicobacter pylori causes gastritis and is an important factor for the development of peptic ulcer disease in man. we used two different methods to examine the adhesion of nine h. pylori strains, with different haemagglutinating properties, to five cell lines, hela s3, hfi, vero, sw1222 and wehi cells. the adhesion studies were performed a) as bacterial adhesion to a monolayer of tissue culture cells, visualizing bacteria with fluorescein-isothiocyanate-labelled antibodies, b) as cell agglutina ... | 1993 | 8280930 |
| identification and characterization of helicobacter pylori phospholipase c activity. | we analyzed 11 h. pylori isolates from humans using the artificial chromogenic substrate paranitrophenylphosphorylcholine to detect phospholipase c (plc) activity. the range of plc in sonicates was 8.8-92.3 (mean 56.9 +/- 6.5) nmol of substrate hydrolysed min-1 mg-1 protein; the amount of activity was not associated with urease or cytotoxin levels. addition of sorbitol or glycerol enhanced plc activity of h. pylori sonicate and purified plc from c. perfringens (plc1) but not purified plc from b. ... | 1993 | 8280931 |
| siderophore production and iron-regulated envelope proteins of helicobacter pylori. | all helicobacter pylori strains tested produced extracellular siderophores, detected by a modified universal detection medium, but growth on this medium was poor. by using the iron chelating compound, 2,2'-dipyridyl, outer membrane proteins of 78 and 40 kda were detected in some, but not all strains examined. no direct binding of lactoferrin or transferrin (the mechanism used by neisseria to obtain iron) could be demonstrated for h. pylori. some other techniques for the study of iron-limitation ... | 1993 | 8280932 |
| chromosomal heterogeneity of helicobacter pylori isolates by pulsed-field gel electrophoresis. | the genome of 24 helicobacter pylori (h. pylori) isolates was analyzed by restriction endonuclease digestion and pulsed-field gel electrophoresis (pfge). on pfge profiles of noti, 12 isolates among 24 yielded four to nine fragments which differed among them. the other 12 isolates could not be digested with noti. the total genome size calculated from individual fragments ranged from 1.04 x 10(3) to 1.80 x 10(3) kb. this remarkable uneveness in the total genome size among isolates is unusual if h. ... | 1993 | 8280933 |
| helicobacter pylori infection within families. | although h. pylori is now well established as the aetiological agent of acute or chronic gastritis and a predisposing factor in peptic ulceration knowledge regarding the transmission of this organism is unclear. the aim of this study was to examine the prevalence of h. pylori infection in the family members of index children infected with this organism and to examine the role of ethnic background and the relationship of hepatitis a infection to h. pylori infection. the h. pylori status of three ... | 1993 | 8280934 |
| helicobacter pylori and gastric malignancy. | 1993 | 8280935 | |
| is helicobacter pylori a causal agent in gastric carcinoma? | the aim of this study is to clarify the relationship between helicobacter pylori (h. pylori) infection and gastric carcinoma. 94 patients with gastric carcinoma and 111 patients with chronic gastritis were involved in this study. they were classified into 3 age groups: group a (40 years and under), group b (41-59), group c (60 years and over). serum samples were tested for h. pylori igg antibodies by elisa and for pepsinogen (pg) by ria. the ratio of pg i/pg ii was used as a marker for atrophic ... | 1993 | 8280936 |
| serology in helicobacter pylori infection. | 1993 | 8280937 | |
| immunisation against gastric infection with helicobacter species: first step in the prophylaxis of gastric cancer? | the discovery of the gastric bacterium, helicobacter pylori and the demonstration of its role in the pathogenesis of gastroduodenal disease, has been one of the major microbiological advances in the last decade. recent demonstration of long term infection with this bacterium as a risk factor in gastric carcinoma suggests that intervention in a disease of major morbidity and mortality is possible. using a model of helicobacter infection in mice it has been shown that oral immunisation with a soni ... | 1993 | 8280938 |
| stimulatory effects of helicobacter pylori on human peripheral blood mononuclear cells of h. pylori infected patients and healthy blood donors. | the ability of 23 different strains of helicobacter pylori to induce proliferative response of human peripheral blood mononuclear cells (pbmc) was investigated. all tested strains stimulated the dna synthesis of pbmc from both healthy and h. pylori infected blood donors, but with lower stimulation of pbmc of infected donors. using different bacterial antigen preparations, such as crude membranes, cytoplasmic proteins, and urease, a significantly lower induction of the proliferative response of p ... | 1993 | 8280939 |
| role of the systemic cellular immune response in the pathogenesis of helicobacter pylori-associated duodenal ulcer. | 20 patients colonized by h. pylori (9 with duodenal ulcer, 11 with antral gastritis) were examined for the presence of a systemic cellular immune response to h. pylori using an in vitro lymphocyte transformation assay. infiltration of the antral mucosa by myelomonocytic cells was assessed by immunohistology as a parameter of local cellular immune response. a systemic h. pylori-induced cellular reaction could be demonstrated in 11 patients all of whom showed an intense myelomonocytic infiltration ... | 1993 | 8280940 |
| humoral and cellular immunity in hiv positive and hiv negative helicobacter pylori infected patients. | the prevalence of h. pylori associated gastritis seems to be different in hiv positive and hiv negative patients. therefore a correlation to immunodeficiency can be postulated. the histology of gastritis, status of h. pylori infection and parameters of humoral and cellular immune response were investigated in 41 hiv positive and 47 hiv negative patients, who were subjected to upper endoscopy for the evaluation of gastrointestinal symptoms. in hiv positive patients 37% had active chronic gastriti ... | 1993 | 8280941 |
| neutralising antibodies to the vacuolating toxin of helicobacter pylori in gastritis only and peptic ulcer patients. | sera from 36 patients with gastritis only and 26 patients with peptic ulcer were tested for the presence of neutralising antibodies against h. pylori vacuolating toxin. the frequency with which sera did neutralise the vacuolating toxin was not significantly different among the groups of patients with gastroduodenal ulcer infected either with toxigenic or nontoxigenic h. pylori strains (9/14 vs 4/12; ns) or in patients with gastritis only harbouring toxigenic h. pylori strains (8/12). by contrast ... | 1993 | 8280942 |
| performance of helicobacter pylori acid extract and urease enzyme-linked immunosorbent assays in relation to 14c-urea breath test. | the 14c-urea breath test has been shown to be a reliable non-invasive method to detect the presence or absence of h. pylori infection. alternatively, a number of techniques have been devised to detect circulating antibodies against h. pylori in serum, the most commonly used being enzyme-linked immunosorbent assays (elisa). in the present study we compared the value of two elisa antigen preparations, an acid glycine extract and a urease preparation, in relation to the results achieved in a 14c-ur ... | 1993 | 8280943 |
| helicobacter pylori antibodies in sera of children suffering from chronic abdominal pain. | 107 pediatric patients aged 9 to 18 with persistent gastric complaints were examined serologically and bacteriologically for helicobacter pylori. helicobacter was identified in 48 (45%) of individuals. 51 (48%) of children were found to be seropositive when h. pylori antibodies were detected by the elisa; 56 (52%) when the passive haemagglutination test was used, and 41 (38%) in the latex agglutination test. 25% of culture-negative patients were found to be seropositive. the percentage of raised ... | 1993 | 8280944 |
| comparison of four second generation kits for detection of igg antibodies against helicobacter pylori in adults. | four serological kits for detection of igg antibodies against h. pylori were tested on 132 adult patients with dyspeptic symptoms. presence of h. pylori infection was established when either culture or microscopy of gastric biopsies were positive. the prevalence of h. pylori infection was 55% in the test population. with anti-h. pylori mtp-assay (roche), pylori stat test kit (whittaker), hm-cap (enteric products inc.) and pyloriset eia-g (orion) the sensitivities were 97%, 95%, 81% and 68% respe ... | 1993 | 8280945 |
| in vitro susceptibility testing of helicobacter pylori to antimicrobial agents: basis for treatment or microbiologists' obsession? | 1993 | 8280946 | |
| antibacterial activity of teprenone, a non water-soluble antiulcer agent, against helicobacter pylori. | teprenone, a non water-soluble antiulcer agent, is a derivative of terpene. the antibacterial activity of teprenone against helicobacter pylori was examined by the disc method and survival test in albumin broth including teprenone. growth of h. pylori around the disc charged with teprenone was inhibited on brucella agar supplemented with bovine serum albumin fraction v, charcoal, or beta-cylodextrin, but not with blood or egg yolk emulsion. furthermore, when h. pylori was exposed in broth includ ... | 1993 | 8280947 |
| therapeutics used to alleviate peptic ulcers inhibit h. pylori receptor binding in vitro. | treatment with bismuth-containing remedies has been long associated with the alleviation of minor gastric ailments. bismuth salts have a potent antimicrobial activity, and are part of the current standard regime used to treat helicobacter pylori infection. h. pylori is considered to be the major etiological factor in the development of peptic ulcer disease. earlier efficacious treatments for peptic ulcer included the oral administration of tween detergents. we have found that these agents have a ... | 1993 | 8280948 |
| effects of substances inhibiting or uncoupling respiratory-chain phosphorylation of helicobacter pylori. | the effects of electron transport inhibitors and uncoupling agents as well as of bismuth compounds on the respiratory activity and oxidative phosphorylation of helicobacter pylori were investigated. bismuth gallate and bismuth subsalicylate reduced the respiratory chain-dependent phosphorylation. inhibition was of the same magnitude as that observed with other known inhibitors. it is concluded that bismuth displays an antibacterial effect by inhibiting the respiratory chain of h. pylori. | 1993 | 8280949 |
| coccoid like forms (clf) of helicobacter pylori. enzyme activity and antigenicity. | in this study we attempted to transform "helical" forms of helicobacter pylori to "coccoid like forms" (clf) by induction with the following substances in vitro: bismuth subcitrate, bismuth subsalicylate, ampicillin, amoxicillin, erythromycin, ursodeoxycholic acid and glycochenodeoxycholic acid. some liquid cultures were incubated for 24 days to induce clf by aging. changes in the protein pattern, urease enzyme activity and in the serological response against specific antigens were investigated. ... | 1993 | 8280950 |
| influence of omeprazole on urease activity of helicobacter pylori in vitro. | the influence of omeprazole on urease activity of 13 helicobacter pylori strains was assessed in vitro employing different inocula of the bacteria and various concentrations of omeprazole. bacteria were grown in liquid culture supplemented with omeprazole for 48 h. afterwards, bacterial numbers were assessed and urease activity was measured in a spectrophotometric assay. in 10 strains, omeprazole had no influence on urease activity at concentrations up to 8 mg/l; higher concentrations had a bact ... | 1993 | 8280951 |
| activity of antibiotics and azole antimycotics against helicobacter pylori. | the bacteristatic and bactericidal activities of six antibiotics from different substance classes against helicobacter pylori were determined. ampicillin, imipenem, tetracycline, and amikacin inhibit the growth of all isolates at concentrations achievable in serum. cefpirome and ofloxacin are ineffective against three and two of 41 strains, respectively. however, the minimum bactericidal concentrations (mbc) of the substances are two- to sixteen-fold higher than the minimum inhibitory concentrat ... | 1993 | 8280952 |
| prevalence of helicobacter pylori in gastrointestinal disorders and concentrations of ciprofloxacin in serum and gastric mucosa. | endoscopic antral biopsies were obtained from 30 patients with several gastrointestinal disorders and analysed for the presence of h. pylori. this bacterium was present in 80% and 58%, respectively, of patients with either gastric and duodenal ulcers and gastric neoplasm. in none of the healthy individuals, h. pylori could be found. another 14 patients with various diseases of the stomach, undergoing a major gastric resection, had been treated with 200 mg ciprofloxacin intravenously immediately ... | 1993 | 8280954 |
| long term infection of the gastric mucosa with helicobacter species does induce atrophic gastritis in an animal model of helicobacter pylori infection. | gastric atrophy is a precursor lesion in the development of gastric cancer. it has been proposed that atrophy is part of a natural progression of inflammatory changes that result from long term infection with the bacterium helicobacter pylori. the aim of this study was to test this hypothesis using an animal model of human helicobacter infection. conventional mice were infected with either a cat isolate of helicobacter felis or a human isolate of "gastrospirillum hominis". all infected mice show ... | 1993 | 8280955 |
| serial change of gastric mucosa after challenging with helicobacter pylori in the cynomolgus monkey. | the aim of this study was to clarify the change of the gastric mucosa following inoculation with helicobacter pylori (h. pylori). two pairs of cynomolgus monkeys received either h. pylori of human origin (group a) or h. pylori of monkey origin (group b) by intragastric inoculation at a dose of 10(9) cfu. after inoculation, endoscopical observation and biopsies were done every 7 days for one month. the bacteria in the biopsy samples were cultured quantitatively. the content of intracellular pas-a ... | 1993 | 8280957 |
| interaction of helicobacter pylori (strain 151) and campylobacter coli with human peripheral polymorphonuclear granulocytes. | helicobacter pylori associated gastritis is characterized by dense mucosal inflammatory infiltrations with predominantly neutrophilic granulocytes, together with a local and systemic immune response. nevertheless, the natural course of the infection is chronic in nature, and active phagocytosis of h. pylori by mucosal granulocytes was only rarely observed. the aim of the present study was to investigate with electronmicroscopic methods the interaction of h. pylori with freshly harvested human pe ... | 1993 | 8280958 |
| the evangelism of helicobacter pylori: how to convince the non-believers and curb the believers. | 1993 | 8280959 | |
| in situ localization of the 60 k protein of helicobacter pylori, which belongs to the family of heat shock proteins, by immuno-electron microscopy. | the groel homologue of helicobacter pylori was isolated and characterized by means of immunoelectron microscopy, after cryosectioning. the 60 k protein was isolated from helicobacter pylori by treatment of the cells with 2-butanol and purified by anion exchange chromatography. the native molecular weight of the 60 k protein was estimated to be 420 k by size exclusion chromatography. the purified 60 k protein showed the typical rotational symmetry of chaperonins when analyzed by electron microsco ... | 1993 | 8280960 |
| activation of human phagocytes by helicobacter pylori. a novel interaction with neutrophils and monocytes distinct from that of n-formylated oligopeptides. | sonicate proteins from helicobacter pylori activate human neutrophils and monocytes for chemotactic and oxidative burst responses. the molecular nature of this activity and its interaction with the phagocyte membrane is unknown. cross-stimulation experiments were performed with human neutrophils and monocytes preincubated in h. pylori sonicate and subsequently stimulated with the established and characterised stimuli n-f-methionyl-leucyl-phenylalanine (fmlp), the complement split product c5a (in ... | 1993 | 8280961 |
| epidemiology of, and risk factors for, helicobacter pylori infection among 3194 asymptomatic subjects in 17 populations. the eurogast study group. | the cross sectional study describes the prevalence of infection with helicobacter pylori as determined by a serodiagnostic assay in over 3000 asymptomatic subjects, in two age groups 25-34 years and 55-64 years, from 17 geographically defined populations in europe, north africa, north america, and japan, using a common protocol for blood collection and serological testing. in all populations combined, the prevalence of infection was higher in the older age group (62.4%) than in the younger age g ... | 1993 | 8282253 |
| prevalence of helicobacter pylori infection and its effect on symptoms and non-steroidal anti-inflammatory drug induced gastrointestinal damage in patients with rheumatoid arthritis. | non-steroidal anti-inflammatory drugs (nsaids) and helicobacter (h pylori) are both associated with an increased risk of peptic ulceration and gastropathy. it is not known, however, if there is an interaction between these two agents, and thus whether or not screening for h pylori before nsaid treatment is of value. the aim of this study was to find out if h pylori potentiates the damaging effects of nsaids. fifty two patients with rheumatoid arthritis requiring longterm nsaid treatment were stu ... | 1993 | 8282254 |
| when is helicobacter pylori infection acquired? | cross sectional surveys have shown an increasing prevalence of helicobacter pylori (h pylori) infection with increasing age in western populations. the aim of this study was to examine the pattern of acquisition of h pylori infection over a 21 year period in a group of 141 adults who had blood samples and serum stored in 1969, 1978, and 1990. a prevalence of h pylori antibody of 39% in 1969 serum samples, 40.9% in 1978, and 34.8% in 1990 was found when assessed by an enzyme linked immunosorbent ... | 1993 | 8282255 |
| lipid analysis of helicobacter pylori. | lipids from helicobacter pylori were extracted, isolated by conventional deae-sephadex and silica gel column chromatography, and then purified by preparative thin-layer chromatography. simple and phospholipids were analyzed by hptlc and quantitatively determined by densitometry scanning. the fatty acid compositions of simple lipids were estimated by gas-liquid chromatography. the simple lipid composition of h. pylori consisted of wax ester (2.5%), triglycerides (4.9%), free fatty acids (30.0%), ... | 1993 | 8283008 |
| significance of serum markers pepsinogen i and ii for chronic atrophic gastritis, peptic ulcer, and gastric cancer. | chronic atrophic gastritis (cag) is closely correlated with gastric cancer and is predominant in japan. epidemiologically, food habits are the primary factor in both cag and gastric cancer. two potential serum markers for cag have recently been investigated, i.e., the concentration of serum pepsinogen (pg) and the presence of serum antibodies against helicobacter pylori. serum pg i and ii and the pg i:pg ii ratio have been reported to be useful as indicators of recurrent peptic ulcer and for scr ... | 1993 | 8283010 |
| [histology of helicobacter pylori (hp) infection: gastric local immunity to hp]. | 1993 | 8283613 | |
| [helicobacter pylori and gastroduodenal diseases: current status]. | 1993 | 8283614 | |
| [bacteriological status, serotypes, and biotypes of helicobacter pylori]. | to divide h. pylori strains into subgroup for an etiological survey, some attempts have been made to determine serotypes or biotypes. a variety of serogroups was observed with a slide agglutination test between s. aureus sensitized with antisera and cells of h. pylori (danielson et al, 1988). three or more serotypes were obtained by using antisera absorbed with heterogeneous strains (tanaka et al, 1990). furthermore, the possible existence of serological subgroups or subtypes by elisa was observ ... | 1993 | 8283615 |
| [lipid composition of helicobacter pylori]. | in h. pylori, we examined the composition of total lipids. in thin-layer chromatography, three kinds of glycolipids were detected, which were named as g-1, g-2 and g-3. g-3 contained phosphates in addition to sugars. total lipids consisted of 6.0% neutral lipids (weight %), 20.6% glycolipids (g-1, g-2) and 73.4% phospholipids (including g-3). total glycolipids were approximately 25% of total lipids. after methanolysis, cholesterol was detected in the hexane extractable fraction and glucose was d ... | 1993 | 8283616 |
| [epidemiological studies and mode of transmission of helicobacter pylori infection]. | recent epidemiological reports of helicobacter pylori (hp) reveal that hp is distributed among all populations in the world. the prevalence rate in the developed countries is generally, lower than that in the developing countries. the prevalence of hp increases with age both in the developed and the developing countries, reflecting a socioeconomic status; the poorer a population, the earlier is the age of infecting hp, resulting in the higher prevalence rate of hp. the epidemiological evidence i ... | 1993 | 8283617 |
| [the possibility of transendoscopic transmission of helicobacter pylori and the method of disinfection of endoscopic instruments]. | although helicobacter pylori (hp) infection has been considered to have an etiologic role in the development of antral gastritis and recurrence of duodenal ulcer, the source and normal route of transmission of hp remains unknown. ironically, iatrogenic infection was the first route of transmission established. through many investigations using hp dna analysis and anti-hp antibody change, the possibility of transendoscopic transmission of hp has been determined. from the fundamental analysis of t ... | 1993 | 8283618 |
| [experimental infection of the cynomolgus monkey with helicobacter pylori]. | to better understand the pathogenesis of helicobacter pylori (h. pylori) associated gastritis and to evaluate the effectiveness of potential therapies in ulcer healing, we established an experimental animal model of h. pylori infection. a strain of h. pylori was isolated from the stomach of a cynomolgus monkey, cultured and used to inoculate as a group of h. pylori negative monkeys. h. pylori-induced changes in the gastric mucosa was monitored both endoscopically and by histological evaluation o ... | 1993 | 8283619 |