Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| plasmid transformation of bacillus cereus on cellophane membranes. | a simple approach to test the ability of bacteria to undergo natural genetic transformation is suggested. the basic feature of the approach is the cultivation of bacterial cells in the presence of exogenous (plasmid) dna on cellophane membranes placed successively on nutrient and selective agar. using this approach the ability of bacillus cereus for "natural" genetic transformation was detected. transformation frequencies varied from 10(-8) to 10(-6). | 1990 | 2126512 |
| forces involved in adhesion of bacillus cereus spores to solid surfaces under different environmental conditions. | the adhesion of bacillus cereus spores (nctc 2599) to hydrophobic and hydrophilic glass surfaces was studied when environmental conditions were varied. the spores were exposed in media of different polarities as well as different ph and ionic concentrations. with increasing ethanol concentrations, the polarity of the medium was decreased and the predominant force of attraction was found to be hydrophobic. the spore surface was uncharged at a ph around 3, at which value the spore was most adhesiv ... | 1990 | 2127266 |
| in vitro activity of sparfloxacin (ci-978; at-4140) against clinical isolates from cancer patients. | the in vitro activity of sparfloxacin, a new quinolone, was compared with those of ciprofloxacin and fleroxacin against gram-positive and gram-negative bacteria, greater than 90% of which were isolated from blood culture specimens of cancer patients. sparfloxacin was extremely active against acinetobacter species, aeromonas hydrophila, citrobacter diversus, enterobacter species, escherichia coli, klebsiella species, proteus vulgaris, and serratia marcescens (inhibiting greater than 90% of these ... | 1990 | 2127348 |
| mercury resistance determined by a self-transmissible plasmid in bacillus cereus 5. | inducible mercuric reductase activity in bacillus cereus 5 was plasmid-encoded. plasmid analysis revealed three plasmids with molecular masses of 2.6, 5.2 and 130 mda. a mating system permitted transfer of the resistance determinant among strains of b. cereus and b. thuringiensis. transfer of mercury resistance from b. cereus 5 to b. cereus 569 and b. thuringiensis occurred during mixed culture incubation on agar surfaces. the 130-mda plasmid (pgb130) was responsible for transfer; frequencies ra ... | 1990 | 2127369 |
| secondary structure of sphingomyelinase from bacillus cereus. | of the total of 306 amino acids in the sequence of sphingomyelinase (smplc) from bacillus cereus, almost half (150) are expected to be involved in the formation of loop or turn structure, while 65 and 73 residues may participate in the formation of alpha-helix and beta-structure, respectively. the helix content of smplc was calculated to be 0-5%, based on the cd spectra. the addition of divalent metal ions such as mg2+ or both ca2+ and mg2+ had no effect on the cd spectra of smplc, although the ... | 1990 | 2127932 |
| metabolism of progesterone and testosterone by bacillus cereus strain socransky 67 and streptococcus mutans strain ingbritt. | bacillus cereus strain socransky 67 and streptococcus mutans strain ingbritt were grown overnight in complex medium and then in fresh medium flasks for 5 h. the bacterial cells in the medium were centrifuged and resuspended; 4-14c-progesterone or 4-14c-testosterone was added, and the samples were incubated for 2 h at 37 degrees c in a shaking water bath. the metabolites were analyzed with column and thin layer chromatography and radioautography and quantified by liquid scintillation counting. on ... | 1990 | 2127957 |
| degranulation of human neutrophils after exposure to bacterial phospholipase c. | because an endogenous phospholipase c (plc) participates in neutrophil activation and because many bacterial pathogens produce plcs, these studies examined the effect of plc from bacillus cereus on the release of the granule enzyme lysozyme from human neutrophils. bacillus cereus plc caused dose-dependent lysozyme release, and combined stimulation of neutrophils with plc and fluoride led to increased secretion. stimulation of neutrophil degranulation is a potential contributing factor for tissue ... | 1990 | 2128277 |
| [sensitivity to antibiotics and selected compounds of bacillus cereus isolated from food products and cases of food poisoning]. | the study was done on 60 strains of bacillus cereus isolated from cases of food poisoning and from food products tested in current supervision. the highest sensitivity was found to the antibiotics: streptomycin, neomycin, gentamicin, amikacin, oxytetracycline, and sulphonamides: negram and sulfathiazole . all strains were resistant to colistin and cloxacillin, nearly all were resistant to penicillin, ampicillin and optochine. | 1990 | 2129276 |
| asymmetric distribution of the phosphatidylinositol-linked phospho-oligosaccharide that mimics insulin action in the plasma membrane. | we have investigated the topography of a glycosyl-phosphatidylinositol implicated in insulin action by a combination of two complementary methods: (a) chemical labelling with a non-permeable (isethionyl acetimidate) and a permeable (ethyl acetimidate) probe; and (b) enzymatic modifications with beta-galactosidase (ec 3.2.1.23) or phosphatidylinositol-specific phospholipase c (ec 3.1.4.3). using the first approach the majority of the glycosyl-phosphatidylinositol is found in the outer surface of ... | 1990 | 2138537 |
| identification and characterization of glycosylphosphatidylinositol-linked schistosoma mansoni adult worm immunogens. | metabolic radiolabeling of adult worms of schistosoma mansoni with [3h]myristic acid has revealed that the fatty acid is incorporated into more than 15 proteins. we have shown that two of these proteins, a 200-kda glycoprotein known to be exposed on the surface of the adult worm following praziquantel treatment and a 22-kda glycoprotein that shows an enhanced immune reactivity with sera of vaccinated mice, are anchored to the adult worm membrane via a glycosylphosphatidylinositol (gpi) linkage. ... | 1990 | 2139172 |
| in vitro activity of ly264826, a new glycopeptide antibiotic, against gram-positive bacteria isolated from patients with cancer. | the in vitro activity of ly264826, a novel glycopeptide antibiotic produced by amycolatopsis orientalis, was compared with those of vancomycin, teicoplanin, and oxacillin against 311 gram-positive clinical isolates from patients with cancer, ly264826 had lower mics for 90% of isolates (mic90) than vancomycin for all species tested. it was active against oxacillin-resistant isolates including staphylococcus aureus (mic90, 0.5 micrograms/ml), staphylococcus haemolyticus (mic90, 2.0 micrograms/ml), ... | 1990 | 2149921 |
| [the effect of glutaraldehyde on mycobacteria and on selected types of bacteria]. | the author tested the action of 2% alkalinized glutaraldehyde on mycobacterium tuberculosis, m. kansasii, m. avium, m. xenopi and m. fortuitum, as well as staphyloccocus aureus, escherichia coli, pseudomonas aeruginosa, proteus vulgaris, bacillus cereus, b. megatherium and b. subtilis. after 5-minute exposure devitalization of m. tuberculosis, m. kansasii and vegetative forms of the tested bacteria occurred. a sporicide effect was recorded already after 30 minutes or after more than 120 minutes. ... | 1990 | 2150610 |
| [products of bacillus cereus contributing to its pathogenicity]. | in a review the author describes the most important exoproducts of strains of bacillus cereus which contribute to its pathogenicity. the toxic product are evaluated with regard to their physical, chemical and biological properties as well with regard to mechanism of their pathogenic action. | 1990 | 2150614 |
| beta-lactamases as fully efficient enzymes. determination of all the rate constants in the acyl-enzyme mechanism. | the rate constants for both acylation and deacylation of beta-lactamase pc1 from staphylococcus aureus and the rtem beta-lactamase from escherichia coli were determined by the acid-quench method [martin & waley (1988) biochem. j. 254, 923-925] with several good substrates, and, for a wider range of substrates, of beta-lactamase i from bacillus cereus. the values of the acylation and deacylation rate constants for benzylpenicillin were approximately the same (i.e. differing by no more than 2-fold ... | 1990 | 2158301 |
| phospholipids chiral at phosphorus. stereochemical mechanism of reactions catalyzed by phosphatidylinositide-specific phospholipase c from bacillus cereus and guinea pig uterus. | (rp)- and (sp)-1,2-dipalmitoyl-sn-glycero-3-thiophosphoinositol (dppsi) were synthesized as a mixture and their configurations assigned on the basis of the stereospecific hydrolysis catalyzed by phospholipase a2 (pla2) from bee venom. pla2 is known to be stereospecific to the rp isomer of 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) and 1,2-dipalmitoyl-sn-glycero-3-thiophosphoethanolamine (dppse). since the configurations of (rp)- and (sp)-dppsi correspond to those of (sp)- and (rp)-d ... | 1990 | 2161255 |
| physical map of the bacillus cereus chromosome. | a physical map of the bacillus cereus chromosome has been constructed by aligning 11 noti fragments, ranging in size from 200 to 1,300 kilobases. the size of the chromosome is about 5.7 megabases. this is the first bacillus genome of which a complete physical map has been described. | 1990 | 2163389 |
| molecular analysis of a sphingomyelinase c gene from leptospira interrogans serovar hardjo. | a thermolabile hemolysin from leptospira interrogans serovar hardjo, strain sponselee, was shown to specifically degrade sphingomyelin. nucleotide sequence determination revealed that sphingomyelinase activity was encoded by an open reading frame of 1,668 nucleotides. although a putative signal sequence could be identified, no evidence for protein export in either l. interrogans or escherichia coli was obtained. the apparent molecular mass of the expression product in e. coli minicells was 41.2 ... | 1990 | 2163985 |
| phosphatidylinositol-specific phospholipase c from bacillus cereus combines intrinsic phosphotransferase and cyclic phosphodiesterase activities: a 31p nmr study. | the inositol phosphate products formed during the cleavage of phosphatidylinositol by phosphatidylinositol-specific phospholipase c from bacillus cereus were analyzed by 31p nmr. 31p nmr spectroscopy can distinguish between the inositol phosphate species and phosphatidylinositol. chemical shift values (with reference to phosphoric acid) observed are 0.41, 3.62, 4.45, and 16.30 ppm for phosphatidylinositol, myo-inositol 1-monophosphate, myo-inositol 2-monophosphate, and myo-inositol 1,2-cyclic mo ... | 1990 | 2175645 |
| determination of phosphatidylcholine in a flow injection system using immobilized enzyme reactors. | two alternative procedures are described for the quantitative determination of phosphatidylcholine in a flow-injection system utilizing immobilized enzymes. phospholipase c from bacillus cereus and phospholipase d from cabbage were covalently bound to the surface of controlled-pore glass beads and the enzyme-derivatized beads were packed in small columns. in the first procedure, the phospholipase c column was connected with a second column containing coimmobilized alkaline phosphatase and cholin ... | 1990 | 2200305 |
| isolation and characterization of a beta-lactamase-inhibitory protein from streptomyces clavuligerus and cloning and analysis of the corresponding gene. | culture filtrates of streptomyces clavuligerus contain a proteinaceous beta-lactamase inhibitor (blip) in addition to a variety of beta-lactam compounds. blip was first detected by its ability to inhibit bactopenase, a penicillinase derived from bacillus cereus, but it has also been shown to inhibit the plasmid puc- and chromosomally mediated beta-lactamases of escherichia coli. blip showed no inhibitory effect against enterobacter cloacae beta-lactamase, and it also showed no activity against a ... | 1990 | 2203736 |
| expression of the blasticidin s deaminase gene (bsr) in tobacco: fungicide tolerance and a new selective marker for transgenic plants. | blasticidin s (bs), a fungicide of microbial origin, is used for the practical control of rice blast disease. it has broad antimicrobial activity but occasionally exhibits adverse phytotoxic effects on some dicot plants. an inactivating enzyme, bs deaminase, was discovered in the bs resistant strain, bacillus cereus k55-s1, and the structural gene, bsr, for the enzyme has been cloned. we introduced the bsr gene into tobacco plants using the ti plasmid vector system and demonstrated that the bsr ... | 1990 | 2250657 |
| asymmetric short-chain phosphatidylcholines: defining chain binding constraints in phospholipases. | several short-chain asymmetric lecithins with a total of 14 carbons in the acyl chains (ranging from 1-lauroyl-2-acetylphosphatidylcholine to 1-hexanoyl-2-octanoylphosphatidylcholine) have been synthesized and characterized. the specific activities of phospholipase a2 from cobra venom, phospholipase a2 from porcine pancreas, and phospholipase c from bacillus cereus toward these lecithins as micelles have been determined. the results of these kinetic studies allow the definition of hydrophobic bi ... | 1990 | 2271632 |
| toxicological, nutritional and microbiological evaluation of tempe fermentation with rhizopus oligosporus of bitter and sweet apricot seeds. | bitter and sweet apricot seeds are by-products of the apricot processing industry. bitter seeds, in particular, contain toxic levels of the cyanogenic substance amygdalin. tempe was made from both kinds of seeds. the bitter seeds contain antimicrobial substances which must be removed by leaching and boiling prior to tempe fermentation. apricot seed tempe had an agreeable taste. it contained approx. 21% (w/w) crude protein, 52% (w/w) crude fat, 1.5% (w/w) crude fibre and 25.5% (w/w) carbohydrates ... | 1990 | 2282289 |
| rapid methods for differentiating gram-positive from gram-negative aerobic and facultative anaerobic bacteria. | different tests based on lysis by koh and on reaction with fluorogenic and chromogenic substrates, l-alanine-4-nitroanilide (lana); l-alanine-4-methoxy- beta-naphthylamide (mna); 4-alanine-2-amidoacridone (aaa); l-alanine-7-amido- 4-methylcoumarin (aamc); 8-anilino-1-naphthalene-sulphonic acid (ans) were compared for their suitability to distinguish gram-positive from gram-negative bacteria. a concentration of 100 micrograms/ml was chosen for incorporating lana, aaa, aamc and ans into the growth ... | 1990 | 2286581 |
| bacterial aetiology of infantile diarrhoea in papua new guinea. | bacterial examination of stools of children with diarrhoea was carried out at port moresby, papua new guinea, where little information is available concerning the causative bacteria of diarrhoea. shigellae, enterotoxigenic escherichia coli, bacillus cereus, staphylococcus aureus, campylobacter jejuni, and aeromonas hydrophila were isolated with the decreasing frequency in that order. among these, enterotoxigenic e. coli and c. jejuni were isolated for the first time in this country. although vib ... | 1990 | 2362308 |
| biological control of damping-off of alfalfa seedlings with bacillus cereus uw85. | we explored the potential of biological control of alfalfa (medicago sativa l.) seedling damping-off caused by phytophthora megasperma f. sp. medicaginis by screening root-associated bacteria for disease suppression activity in a laboratory bioassay. a total of 700 bacterial strains were isolated from the roots of field-grown alfalfa plants by using trypticase soy agar. a simple, rapid assay was developed to screen the bacteria for the ability to reduce the mortality of iroquois alfalfa seedling ... | 1990 | 16348145 |
| the effect of microwave and conventional cooking on the temperature profiles and microbial flora of minced beef. | beef mince samples were cooked to the rare, medium and well done states by microwave and conventional oven methods. the raw samples all contained large numbers of contaminating organisms and low numbers of listeria monocytogenes were detected in the majority of samples. a substantial proportion of the contaminating flora survived in mince cooked by microwaves to the rare, medium and well done states, prior to a 30-min standing period. listeria monocytogenes survived in all samples cooked by micr ... | 1991 | 1742174 |
| microbiological analysis and starter culture growth in retentates. | pasteurized skim milk was concentrated by uf to 2-, 4-, and 5-fold. the retentates were evaluated for microbiological quality, heat treatments to inactivate microorganisms, and lactic acid bacterial starter culture activity. aerobic mesophilic bacterial counts in raw milk decreased from an initial 1.4 x 10(6) to 3.9 x 10(2) cfu/ml after pasteurization. during uf, counts increased from 3.9 x 10(2) cfu/ml uf, counts increased from 3.9 x 10(2) cfu/ml in pasteurized milk to 1.4 x 10(3), 1.4 x 10(4), ... | 1991 | 1744259 |
| amperometric monitoring of bacteria-induced milk acidity using a platinum disc microelectrode. | the acidity induced by the action of bacteria in milk samples was monitored amperometrically by using a platinum microelectrode. the measurements were performed directly on commercial packs of milk, stored at 32 degrees c, and were continued for 9-10 d after inoculation. the data were compared with those obtained by measuring the ph of the samples and the results are discussed on the basis of the metabolism of each bacterial species. the effects of the following bacteria were examined: staphyloc ... | 1991 | 1759720 |
| proline residues responsible for thermostability occur with high frequency in the loop regions of an extremely thermostable oligo-1,6-glucosidase from bacillus thermoglucosidasius kp1006. | the gene encoding for an extremely thermostable oligo-1,6-glucosidase from bacillus thermoglucosidasius kp1006 (dsm2542, obligate thermophile) was sequenced. the amino acid sequence deduced from the nucleotide sequence of the gene (1686 base pairs) corresponded to a protein of 562 amino acid residues with a mr of 66,502. its predicted amino acid composition, mr, and n-terminal sequence of 12 residues were consistent with those determined for b. thermoglucosidasius oligo-1,6-glucosidase. the dedu ... | 1991 | 1761534 |
| enhancement of soybean nodulation by bacillus cereus uw85 in the field and in a growth chamber. | seed treatments with bacillus cereus uw85 increased nodulation of soybeans in three field seasons and in three different sterilized soils in the growth chamber. in the field, 28 and 35 days after planting, uw85-treated plants had 31 to 133% more nodules than untreated plants. from 49 days after planting until seed harvest, there were no significant differences between nodulation of uw85-treated plants and untreated control plants. in the growth chamber, in sterilized soil-vermiculite mixtures, a ... | 1991 | 1768151 |
| [effects of sodium chloride on destruction of microorganisms by microwave heating in potatoes]. | to assess the destructive effect of different sodium chloride concentrations (0, 0.3, 1.5, and 3%) on microorganisms with microwave heating, strains of vibrio parahaemolyticus, staphylococcus aureus, salmonella enteritidis, escherichia coli and bacillus cereus were used to inoculate a mashed potato preparation. after microwave heating for 1 min at 800 w, resulted in temperatures and rate of destruction of bacteria significantly higher (p less than 0.05) in the core than on the surface of mashed ... | 1991 | 1773068 |
| purification and some properties of phospholipase c from achromobacter xylosoxidans. | a non-haemolytic phospholipase c (ec 3.1.4.3) was purified from the culture medium of achromobacter xylosoxidans with a 5% yield and a purification factor of 330. a combination of ultrafiltration, acetone precipitation and two subsequent affinity chromatographic steps was used. the affinity chromatography is a new application of 2-(4-aminophenylsulphonyl)ethyl-cellulose, a sorbent that has previously been used for the purification of phospholipase c from bacillus cereus. the purified enzyme gave ... | 1991 | 1783637 |
| [effect of escherichia coli on growth of bacillus cereus]. | the effect of 101 escherichia coli strains on growth of 90 bacillus cereus strains on solid media was investigated. only 9 e. coli strains (in particular the colicin +-generating ones) were antagonistic towards b. cereus, giving distinct growth-inhibition zones around the colonies. | 1991 | 1788515 |
| phospholipase c-sensitive factor vii complexes in dog plasma. | we report the presence of a phospholipase c-sensitive activated factor vii complex in canine plasma after feeding a special diet. low levels of complex was observed in the fasting state. the response to feeding in terms of activated factor vii varied markedly among the dogs investigated. | 1991 | 1788825 |
| a fluorescent substrate for the continuous assay of phosphatidylinositol-specific phospholipase c: synthesis and application of 2-naphthyl myo-inositol-1-phosphate. | a fluorescent water-soluble substrate for phosphatidylinositol-specific phospholipase c was synthesized. the diacylglycerol moiety of the natural substrate, phosphatidylinositol, was replaced by the fluorescent moiety, 2-naphthol, resulting in the synthetic substrate, racemic 2-naphthyl myo-inositol-1-phosphate. the synthetic substrate provided a continuous fluorometric assay for the phosphatidylinositol-specific phospholipase c from bacillus cereus. initial rates of the cleavage of the 2-naphth ... | 1991 | 1789410 |
| ph gradients through colonies of bacillus cereus and the surrounding agar. | ph-sensitive microelectrodes, constructed with a tip diameter of about 4 microns, were deployed through 24 h and 48 h colonies of bacillus cereus incubated on cys medium (casamino acids, yeast extract, salts), with and without glucose. measurements of ph were used to construct ph profiles through the colony and the surrounding agar. ph gradients could be detected for at least 800 microns into the agar beneath a 24 h colony, and to approximately 10 mm horizontally away from the edge of the colony ... | 1991 | 1791442 |
| results in the management of bacillus endophthalmitis. | eighteen cases of culture-proven bacillus endophthalmitis were reviewed. the majority (13/18) were secondary to penetrating ocular trauma. others were secondary to corneal ulcer (1), postoperative (1), and endogenous (3). bacillus cereus was the most commonly identified species (12/18). antibiotic sensitivities demonstrated uniform sensitivity to vancomycin and the aminoglycosides. visual prognosis was generally poor; only two patients maintained 20/200 or better in the affected eye. fourteen ey ... | 1991 | 1792034 |
| hydroxylation and glucoside conjugation in the microbial metabolism of the diterpene sclareol. | 1. the microbial metabolism of sclareol (1), a labdane diterpene ditertiary alcohol, was studied. preliminary screening identified a number of microorganisms capable of metabolizing sclareol. 2. preparative scale fermentation of growing cultures of bacillus cereus ui-1477 resulted in the production of seven metabolites which have been characterized as 3 beta-hydroxysclareol (2), 2 alpha-hydroxysclareol (3), 18-hydroxysclareol (4), 2 alpha,18-dihydroxysclareol (6), 8 alpha,13 beta-dihydroxy-labd- ... | 1991 | 1796608 |
| isolation and purification of bacterial proteinases by means of autofocusing. | autofocusing was used for the isolation and purification of neutral and alkaline proteinases from fermentation broth, after separation of cells. the yield of proteinases achieved was 19-78%, and was inversely proportional to the degree of purification, which varied from 3.0 to 7.9. because of considerable losses of enzyme activity and the long duration of the process, autofocusing seems to be a non-economic technique for industrial isolation of relatively cheap enzymes. | 1991 | 1804847 |
| a chromogenic substrate for phosphatidylinositol-specific phospholipase c: 4-nitrophenyl myo-inositol-1-phosphate. | a chromogenic water-soluble substrate for phosphatidylinositol-specific phospholipase c was synthesized starting from myo-inositol employing isopropylidene and 4-methoxytetrahydropyranyl protecting groups. in this analogue of phosphatidylinositol, 4-nitrophenol replaces the diacylglycerol moiety, resulting in synthetic, racemic 4-nitrophenyl myo-inositol-1-phosphate. using this synthetic substrate a rapid, convenient and sensitive spectrophotometric assay for the phosphatidylinositol-specific ph ... | 1991 | 1814635 |
| role of side chain moiety in the hydrolysis of penicillins by beta-lactamase. | various beta-lactam compounds and structurally related moieties were examined as substrates of beta-lactamase from bacillus cereus 5/b nctc 9946. the enzyme was specific for penicillins and none of the cephalosporins were hydrolysed. electronic environment of allylic carboxy group in dihydrothiazine ring restricts the acceptance of cephalosporins as substrates. the efficiency of hydrolysis of penicillins is dependent on dense resonating electronic environment of phenyl ring present in the side c ... | 1991 | 1814862 |
| hyperexpression in escherichia coli, purification, and characterization of the metallo-beta-lactamase of bacillus cereus 5/b/6. | we used site-directed mutagenesis to introduce both a ndei restriction endonuclease site and an initiator codon at the junction of the leader and structural gene sequences of the metallo-beta-lactamase of bacillus cereus 5/b/6. this construct allowed us to clone just the beta-lactamase structural gene sequence into an escherichia coli expression vector. e. coli cells were transformed with the recombinant plasmid, the b. cereus beta-lactamase was expressed, and these e. coli cells were disrupted ... | 1991 | 1821784 |
| fluid accumulation in mouse ligated intestine inoculated with the vascular permeability factor produced by bacillus cereus. | partially purified vascular permeability (vp) factor (vpf) of bacillus cereus induced fluid accumulation in the ligated intestinal loops of mouse (mil) and rabbit (ril), suggesting that the vp activity may correlate with fluid accumulation in ligated intestinal loops of these animals. fluid accumulation was observed at 6-8 hr in 55-67% of mouse intestinal loops inoculated with 40-50 immunodiffusion units (idu) of partially purified vpf, whereas it was found at 2 hr in all loops with 400-600 idu ... | 1991 | 1830786 |
| purification and characterization of the vascular permeability factor produced by bacillus cereus. | purification of an extracellular protein exhibiting the vascular permeability activity produced by bacillus cereus was performed by ammonium sulfate precipitation followed by chromatography on de-32 cellulose, sephadex g-100, and sephadex g-75. the purified protein was found to be electrophoretically and antigenically almost homogeneous although it contained a trace of contaminant. the molecular weight of the protein was calculated to be 45,000 by polyacrylamide gel electrophoresis in the presen ... | 1991 | 1830799 |
| in vitro stability in biological activity and antigenicity of the vascular permeability factor produced by bacillus cereus. | 1991 | 1830805 | |
| development of murine monoclonal antibodies against an enterotoxin produced by bacillus cereus. | three murine monoclonal antibodies (mabs) were prepared against an enterotoxin (et) produced by bacillus cereus. although these mabs were found to react with the et, their specificities appeared to be different in competitive binding assays. one of the mabs (d-8), which was highly reactive with the et, will be useful in developing immunological methods to detect crude et and to isolate the et in high yield. | 1991 | 1832030 |
| purification and some properties of a bacillus cereus mouse lethal toxin. | a mouse lethal toxin (mlt) produced by bacillus cereus isolated in vomiting-type food poisoning was purified by chromatography on deae-sephadex a-25 followed by gel filtration on sephadex g-75. purified mlt possessed a molecular weight of 33,000-34,000. it showed mouse lethality and hemolytic (hl) activity on sheep and rabbit erythrocytes; the latter erythrocytes were more weakly hemolyzed than the former ones. however, fluid accumulation in mouse ligated intestinal loops was not induced by puri ... | 1991 | 1832034 |
| adsorption on bacterial spores depending on the aggregation properties of antimicrobial tensides. | a change in interaction with spores of bacillus cereus occurred in the range of critical concentrations of micelle formation. with 1-methyldodecyldimethylamine-n-oxide and n,n'-bis(dodecyldimethyl)-1,2-ethanediammonium dibromide, the induced release of dipicolinic acid was blocked and the adsorption dynamics changed, respectively. | 1991 | 1841878 |
| mechanism of inhibition of adenylate cyclase by phospholipase c-catalyzed hydrolysis of phosphatidylcholine. involvement of a pertussis toxin-sensitive g protein and protein kinase c. | the phospholipase c-mediated hydrolysis of phosphatidylcholine has been shown recently to be activated by a number of agonists. muscarinic receptors, which trigger various signal transduction mechanisms including inhibition of adenylate cyclase through gi, have been shown to be potent stimulants of this novel phospholipid degradative pathway. we demonstrate here, by exogenous addition of bacillus cereus phosphatidylcholine-hydrolyzing phospholipase c, that phosphatidylcholine breakdown mimics th ... | 1991 | 1845988 |
| purification and characterization of two-subunit cytochrome aa3 from bacillus cereus. | cytochrome c-oxidase type aa3 (ec 1.9.3.1) was purified to homogeneity from vegetative bacillus cereus by ion-exchange and hydroxylapatite chromatography in the presence of triton x-100. gel filtration analysis suggested a dimeric structure apparently 172 kda in size; however, only a monomer of 81 kda was detected when analysed by non-denaturing gel electrophoresis. denaturing gel electrophoresis analysis of the protein showed the presence of two subunits (51 and 30 kda). atomic absorption and v ... | 1991 | 1849607 |
| the aeromonas hydrophila cpha gene: molecular heterogeneity among class b metallo-beta-lactamases. | an aeromonas hydrophila gene, named cpha, coding for a carbapenem-hydrolyzing metallo-beta-lactamase, was cloned in escherichia coli by screening an aeromonas genomic library for clones able to grow on imipenem-containing medium. from sequencing data, the cloned cpha gene appeared able to code for a polypeptide of 254 amino acids whose sequence includes a potential n-terminal leader sequence for targeting the protein to the periplasmic space. these data were in agreement with the molecular mass ... | 1991 | 1856163 |
| travelers' diarrhea among united states military personnel during joint american-egyptian armed forces exercises in cairo, egypt. | a study was conducted of travelers' diarrhea in a united states military population on deployment in cairo, egypt, during july and august 1987. acute diarrhea requiring medical attention developed in 183 (4%) of 4,500 troops. a possible etiologic agent was identified in 49% of all diarrhea cases. enteric pathogens associated with cases of diarrhea included: enterotoxigenic escherichia coli (17% st-producers, 13% lt-producers, and 3% lt/st-producers); shigella (9%); campylobacter spp. (2%); salmo ... | 1991 | 1900113 |
| production of 2-methyl-4[3h]-quinazolinone, an inhibitor of poly(adp-ribose) synthetase, by bacterium. | 1991 | 1900501 | |
| periodic acid-schiff-positive organisms in primary cutaneous bacillus cereus infection. case report and an investigation of the periodic acid-schiff staining properties of bacteria. | primary cutaneous bacillus cereus infection frequently presents as a single necrotic bulla on the extremity of an immunocompromised patient. in lesional biopsy specimens and smears, the large gram-positive rods of b cereus may be mistaken for clostridium species. this is a potentially serious error, as bacillus species are resistant to penicillin and other beta-lactam antibiotics. we studied a case in which large periodic acid-schiff-staining organisms were seen in the biopsy specimen from a nec ... | 1991 | 1900984 |
| in vitro susceptibilities of ocular bacillus cereus isolates to clindamycin, gentamicin, and vancomycin alone or in combination. | a broth dilution assay was used to determine the in vitro susceptibilities of 10 ocular isolates of bacillus cereus to clindamycin, gentamicin, and vancomycin both alone and in combination. the checkerboard technique was used to determine fractional inhibitory and bactericidal concentration indices for combinations of clindamycin-gentamicin and vancomycin-gentamicin. | 1991 | 1901697 |
| structural and stereospecific requirements for the nucleoside-triggered germination of bacillus cereus spores. | a selection of adenosine analogues was tested for their ability to trigger germination of bacillus cereus ncib 8122 spores. the germination-inducing activity was governed by the structural properties of the sugar rather than the base moieties of the nucleosides. among the sugar-modified analogues, only those containing a 2'-deoxy-d-ribose moiety promoted spore germination. requirements for a specific molecular structure of the base were not clearly identified, although the highest activity was o ... | 1991 | 1901905 |
| further observations on enhancement of the infectivity of fusobacterium necrophorum by other bacteria. | it had already been shown with a single virulent strain (a42) of fusobacterium necrophorum that suspension of the fusobacteria in sub-lethal doses of broth cultures of other bacteria reduced the minimum infective dose (greater than 10(6) organisms) for mice by subcutaneous inoculation, sometimes to less than 10 organisms. the present study extended the known range of bacteria with strong infectivity-enhancing properties to include bacillus cereus, klebsiella oxytoca and staphylococcus aureus; an ... | 1991 | 1902184 |
| characterization of the components of hemolysin bl from bacillus cereus. | previously we described the partial purification of a novel hemolysin from bacillus cereus and showed that hemolytic activity required the combined action of at least two components, called b and l to signify their cell-binding and cell-lytic roles in this activity. on further purification, as described in the present article, a combination of anion-exchange chromatography and polyacrylamide gel electrophoresis separated three proteins, b, l1, and l2 (35, 36, and 45 kda, respectively). individua ... | 1991 | 1902196 |
| hemolytic and sphingomyelinase activities of clostridium perfringens alpha-toxin are dependent on a domain homologous to that of an enzyme from the human arachidonic acid pathway. | the n-terminal domain of clostridium perfringens alpha-toxin, homologous with the nontoxic phospholipase c of bacillus cereus, was expressed in escherichia coli and shown to retain all of the phosphatidylcholine hydrolyzing activity of the alpha-toxin, but not the sphingomyelinase, hemolytic, or lethal activities. the c-terminal domain of alpha-toxin showed sequence and predicted structural homologies with the n-terminal region of arachidonate 5-lipoxygenase, an enzyme from the human arachidonic ... | 1991 | 1902199 |
| mureidomycin a, a new inhibitor of bacterial peptidoglycan synthesis. | mureidomycin a (mrd), a novel peptidylnucleoside antibiotic with antipseudomonal activity, inhibited not only peptidoglycan synthesis but also lipid-intermediate formation from udp-n-acetylmuramyl (murnac)-pentapeptide and udp-n-acetylglucosamine in an in vitro peptidoglycan-synthesizing system, using ether-treated cells of pseudomonas aeruginosa. both types of inhibition by mrd disappeared when udp-murnac-pentapeptide was preincubated with ether-treated cells. moreover, mrd completely inhibited ... | 1991 | 1902646 |
| inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase c. | the effects of phorbol 12-myristate 13-acetate (pma), a potent activator of protein kinase c (pkc), on high-affinity na(+)-dependent gamma-aminobutyric acid (gaba) uptake were investigated in primary cultures of neurons and glial cells from rat brain cortex. incubation of glial cells with pma led to concentration- and time-dependent decreases in the gaba transport in glial cells. this effect could be completely suppressed by addition of the pkc inhibitor h7. the pma effects could be mimicked by ... | 1991 | 1902665 |
| bacillus cereus: a significant pathogen in postoperative and post-traumatic wounds on orthopaedic wards. | over a period of 19 months, bacillus species, most frequently b. cereus, were isolated from postoperative and post-traumatic wounds in 24 patients on orthopaedic wards in a swedish hospital. clinical signs of infection were found in all but 3 patients: in 9 patients, mild signs with increased secretion from the wounds; in 10 patients, moderate signs with fever and/or significant local reaction around the wounds; and in 2 patients, severe signs with necrotic infections. bacillus spp. were isolate ... | 1991 | 1902983 |
| successful treatment of bacillus cereus infection with ciprofloxacin. | bacillus cereus is rarely a pulmonary pathogen but may cause pneumonia in immunocompromised patients. a patient with bronchiectasis and no recognisable immunodeficiency had this organism isolated during two infective exacerbations, once from respiratory secretions and once by blood culture. ciprofloxacin treatment was effective on both occasions. | 1991 | 1902995 |
| effects of salt concentration on bacterial growth on plates with gradients of ph and temperature. | a temperature gradient device has been used to produce ph-temperature gradient plates. the response of salmonella typhimurium and bacillus cereus to continuous gradients of ph and temperature has been mapped. a third variable, sodium chloride, was investigated by incorporating it at different concentrations in a series of gradient plates. both organisms exhibited an optimum temperature and ph range for maximum salt tolerance. | 1991 | 1903754 |
| envelope alteration of escherichia coli hb101 carrying peap31 caused by kil peptide and its involvement in the extracellular release of periplasmic penicillinase from an alkaliphilic bacillus. | the plasmid peap31 contains the colicin e1 kil gene. peptidoglycan and outer-membrane components (lipopoly-saccharide, proteins and phosphatidylethanolamine) decreased concurrently in the envelope fraction from escherichia coli hb101 carrying peap31 during the stationary phase of growth. at almost the same time. d-alanine residues in peptidoglycan decreased. the kil peptide is suggested to affect, directly or indirectly, the turnover of peptidoglycan in stationary phase and, as a result, to caus ... | 1991 | 1903920 |
| phosphonate monoester inhibitors of class a beta-lactamases. | phosphonate monoesters with the general structure: [formula: see text] are inhibitors of representative class a and class c beta-lactamases. this result extends the range of this type of inhibitor to the class a enzymes. compounds where x is an electron-withdrawing substituent are better inhibitors than the unsubstituted analogue (x = h), and enzyme inhibition is concerted with stoichiometric release of the substituted phenol. slow turnover of the phosphonates also occurs. these observations sup ... | 1991 | 1903928 |
| a continuous fluorescence-displacement assay for triacylglycerol lipase and phospholipase c that also allows the measurement of acylglycerols. | a new continuous fluorescence-displacement assay for enzymes that release long-chain fatty acids [wilton (1990) biochem. j. 266, 435-439] is described in detail for pig pancreatic triacylglycerol lipase. the assay involves the displacement of the highly fluorescent fatty acid probe 11-(dansylamino)undecanoic acid from rat liver fatty acid-binding protein by long-chain fatty acids released as a result of enzyme activity. the assay is surprisingly effective for triacylglycerol lipase, allowing the ... | 1991 | 1903930 |
| one-step purification procedure for udp-n-acetylmuramyl-peptide murein precursors from bacillus cereus. | a method is described for the rapid isolation of the activated murein precursors udp-n-acetyl-muramyl-pentapeptide (udp-murnac-pentapeptide) and udp-murnac-tripeptide from bacillus cereus. after accumulation of the precursors by inhibition of murein synthesis either in the presence of vancomycin (for the pentapeptide precursor) or d-cycloserine (for the tripeptide precursor) the cells were extracted with boiling water. prior to high pressure liquid chromatography the material was freed from acid ... | 1991 | 1904044 |
| nonanthrax bacillus infections of the central nervous system. | 1991 | 1904148 | |
| effect of phenolic compounds and oleuropein on the germination of bacillus cereus t spores. | the phenolic compounds extracted from olives with ethyl acetate inhibited germination and outgrowth of bacillus cereus t spores. purified oleuropein, a well-characterized component of olive extract, inhibited these processes also. the addition of oleuropein and olive extracts 3 or 5 min after germination began, immediately decreased the rate of change of phase bright to phase dark spores and delayed significantly outgrowth. | 1991 | 1904245 |
| site-directed mutagenesis of dicarboxylic acids near the active site of bacillus cereus 5/b/6 beta-lactamase ii. | an amino acid residue functioning as a general base has been proposed to assist in the hydrolysis of beta-lactam antibiotics by the zinc-containing bacillus cereus beta-lactamase ii [bicknell & waley (1985) biochemistry 24, 6876-6887]. oligonucleotide-directed mutagenesis of cloned bacillus cereus 5/b/6 beta-lactamase ii was used in an 'in vivo' study to investigate the role of carboxy-group-containing amino acids near the active site of the enzyme. substitution of asparagine for the wild-type a ... | 1991 | 1904717 |
| assays of phospholipases on short-chain phospholipids. | short-chain phospholipids are extremely useful compounds for analysis of interfacial and substrate requirements of water-soluble phospholipases. a wide variety of pcs are easily prepared and can be characterized in detail to the point where at a given pc concentration, the micelle size distribution is known, and an area per head group can be estimated. since they can be presented to enzymes as monomers, micelles, or in bilayer structures, short-chain phospholipids are ideal for examining kinetic ... | 1991 | 1904976 |
| differentiation of dairy strains of the bacillus cereus group by phage typing, minimum growth temperature, and fatty acid analysis. | a total of 130 bacillus strains were isolated from dairy products, the dairy environment and from packaging boards and board-producing machines. ninety-eight of these were members of the b. cereus group (b. cereus, b. mycoides and b. thuringiensis) as determined by whole cell fatty acid composition. fatty acid composition did not differentiate between the three species. of the 98 strains, which were indistinguishable by biochemical tests, 87 could be assigned into 21 different phage types (11 st ... | 1991 | 1905282 |
| growth of bacillus cereus in fermenting tempeh made from various beans and its inhibition by lactobacillus plantarum. | counts of bacillus cereus reached ca 10(8) cfu/g within 40 h in fermenting unacidified horsebean tempeh and resulted in complete spoilage of the product. in fermenting unacidified pea, chickpea and soybean tempeh, b. cereus counts reached 10(6)-10(7) cfu/g, although the products were not spoiled. inoculation of these unacidified beans with lactobacillus plantarum decreased the final count of b. cereus by 2 log units, but had no effect on its growth in unacidified horsebean tempeh and its subsequ ... | 1991 | 1905283 |
| identity of hemolysins produced by bacillus thuringiensis and bacillus cereus. | a hemolysin (bt-hemolysin) produced by bacillus thuringiensis var. kurstaki hd-1 producing crystalline toxin(s) was purified by successive treatments of ammonium sulfate (45-65%) and column chromatography using deae-cellulose, sephadex g-75 and kb-002 (a hydroxyapatite column for fast protein liquid chromatography). a hemolysin (bc-hemolysin) produced by b. cereus hg-6a was also purified by the same procedure. the purified bt-hemolysin and bc-hemolysin, both of which are thiol-activated hemolysi ... | 1991 | 1905656 |
| irreversible inactivation of beta-lactamase i from bacillus cereus by chlorinated 6-spiroepoxypenicillins. | on incubation of the chlorinated 6-spiroepoxypenicillin anilides (i) and (ii) [formula: see text] with beta-lactamase 1 from bacillus cereus, three distinct processes are observed. the inhibitors act as (a) substrates, the turnover of which respectively results in a single product, namely 6-substituted 2(h)-3,4-dihydro-1,4-thiazine, (b) a transiently inhibited enzyme complex, and finally (c) an irreversibly inactivated enzyme complex. although differing only in their stereochemistry at one centr ... | 1991 | 1905929 |
| antimicrobial activity of microgard against food spoilage and pathogenic microorganisms. | microgard, a commercially available fermented milk product containing antimicrobial metabolites, was a potent inhibitor for gram-negative bacteria such as pseudomonas, salmonella, and yersinia when 1% concentration was incorporated into agar media. gram-positive bacillus cereus, staphylococcus aureus, and listeria monocytogenes were insensitive to microgard. kluyveromyces marxianus, an unidentified black yeast, and penicillium expansum were partially suppressed, whereas aspergillus niger and a y ... | 1991 | 1906486 |
| bacillus cereus pneumonia with empyema complicating aplastic anemia--a case report. | 1991 | 1906515 | |
| improved methods for purification of an enterotoxin produced by bacillus cereus. | an enterotoxin (et) produced by bacillus cereus was purified by ammonium sulfate precipitation followed by chromatography on sp-sephadex c-25, chromatofocusing, and gel filtration on sephacryl s-300. purified et was electrophoretically homogeneous with a molecular mass of 45,000 and with an isoelectric point of 5.5. it showed vascular permeability activity in rabbits, was lethal to mice, and caused fluid accumulation in mouse ligated intestinal loops. it showed cytotoxicity to vero cells, but di ... | 1991 | 1906824 |
| [the effect of a cationic surface-active substance (catamine ab) on the physiological-morphological properties of b. cereus and e. coli]. | catamine ab (0.05-0.5%) promotes transfer of b. cereus st 96, and e. coli st. 906 cell cultures into metabolic rest. detergent-treated cells have no energetic metabolism and autolytic processes, have high light-scattering coefficient, and peculiar ultrastructural organization. viable cells can be observed in the detergent-treated cell suspension after 1 year of incubation. difference in action of different catamine ab concentrations on stationary and exponential b. cereus cells has been revealed ... | 1991 | 1906901 |
| inhibition of clostridium tyrobutyricum by bacteriocin-like substances produced by lactic acid bacteria. | lactic acid bacteria were selected for their inhibitory activity against clostridium tyrobutyricum under conditions that eliminate the effects of lactic acid and hydrogen peroxide. four strains were isolated belonging to the species lactococcus lactis ssp. lactis. the sensitivity of the inhibitory substances to pronase and trypsine indicates that they are proteins or peptides different from nisin. their resistance to phospholipase d indicates that they are also different from lactostrepcin. the ... | 1991 | 1907300 |
| influence of cellular differentiation on ultraviolet induced dna damage and its repair mechanisms in b. cereus. | susceptibility to uv irradiation of b. cereus bis-59 spores undergoing germination at various stages-dormant spores to vegetative cell stage and their ability to recover from radiation damage were studied. for a given dose of radiation, the number of spore photoproducts (spp) formed in the dna of dormant spores was about 5-times greater than that of thymine dimers (tt) formed in the dna of vegetative cells. at intermediate stages of the germination cycle, there was a rapid decline in the uv radi ... | 1991 | 1908819 |
| differentiation of bacillus anthracis from bacillus cereus by gas chromatographic whole-cell fatty acid analysis. | three strains of bacillus anthracis and seven strains of bacillus cereus were grown on complex medium and on synthetic medium. gas chromatographic analysis of whole-cell fatty acids of strains grown on complex medium gave nearly identical fatty acid patterns. fatty acid patterns of strains grown on synthetic medium showed a high content of branched-chain fatty acids. significant differences between the fatty acid patterns of the two species were found. odd iso/anteiso fatty acid ratios were abou ... | 1991 | 1909348 |
| the occurrence and growth of microorganisms during the fermentation of fish sausage. | minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ... | 1991 | 1909546 |
| microbial conversion of fungicide vinclozolin. | an ecological safety study of using vinclozolin in field and laboratory experiments showed that the effect of the preparation led to a decrease in the abundance of actinomycetes and mycelial fungi and an enhancement of nitrification. the residual amounts of vinclozolin in soil after 12 months were 6-12% of the dose introduced. the persistent chlorinated derivatives of the toxicant were found. microbial strains pertaining to the genera pseudomonas and bacillus were isolated that utilized vinclozo ... | 1991 | 1910065 |
| overproduction, purification and crystallization of bacillus cereus oligo-1,6-glucosidase. | the gene coding for oligo-1,6-glucosidase from bacillus cereus atcc7064 has been overexpressed in escherichia coli mv1184 cells under the control of the lac promoter in the genetically engineered plasmid pbce4-2. oligo-1,6-glucosidase was purified in large quantities and was crystallized at 25 degrees c by using a hanging drop vapor diffusion method with 53% saturated ammonium sulfate. the crystals have the shape of hexagonal bipyramids and belong to the space group p6(2) or p6(4) with lattice c ... | 1991 | 1915879 |
| bacteria in food packaging paper and board. | the bacteria of food packaging paper and board were studied. most of the aerobic strains were spore-formers; members of the genus bacillus with b. cereus group (b. cereus, b. mycoides, b. thuringiensis), b. polymyxa group (b. polymyxa, b. circulans, b. macerans, b. pabuli), b. brevis and b. licheniformis predominated. the main source of spore-forming bacteria in paper and board was the broke (rejected paper or board, which is repulped and recycled into the process). gram-negative bacteria were r ... | 1991 | 1917722 |
| symposium on microbiology update: old friends and new enemies. bacillus cereus. | bacillus cereus is an environmentally ubiquitous, gram-positive, spore-forming bacillus responsible for 2 distinct foodborne disease syndromes as well as other manifestations of pathogenicity. the rapid-onset, "emetic," foodborne-disease syndrome is associated with an emetic toxin; the delayed-onset, "diarrheal" syndrome is associated with elaboration of enterotoxin. the majority of methods for detection of these toxins have relied on in vivo testing. more recent work on purification of enteroto ... | 1991 | 1917820 |
| ultraviolet irradiation of dna complexed with alpha/beta-type small, acid-soluble proteins from spores of bacillus or clostridium species makes spore photoproduct but not thymine dimers. | uv irradiation of complexes of dna and an alpha/beta-type small, acid-soluble protein (sasp) from bacillus subtilis spores gave decreasing amounts of pyrimidine dimers and increasing amounts of spore photoproduct as the sasp/dna ratio was increased. the yields of pyrimidine dimers and spore photoproduct were less than 0.2% and 8% of total thymine, respectively, when dna saturated with sasp was irradiated at 254 nm with 30 kj/m2; in the absence of sasp the yields were reversed-4.5% and 0.3%, resp ... | 1991 | 1924287 |
| pericarditis due to bacillus cereus in an intravenous drug user. | 1991 | 1925304 | |
| comparative study of various hydrogen ion buffers to assay zn(2+)-dependent beta-lactamases. | the low zn2+ complex formation constants, the capacity to degrade penicillin g in combination with zn2+, and uv absorbance make 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 3-[n-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid, 1,3-bis[tris(hydroxymethyl)methylamino]propane, and 1,4-piperazinediethanesulfonic acid convenient buffers to study zn(2+)-dependent beta-lactamases. | 1991 | 1929321 |
| interaction of beta-lactamases i and ii from bacillus cereus with semisynthetic cephamycins. kinetic studies. | the influence of c-6 alpha- or c-7 alpha-methoxylation of the beta-lactam ring in the catalytic action of class a and b beta-lactamases has been investigated. for this purpose the kinetic behaviour of beta-lactamases i (class a) and ii (class b) from bacillus cereus was analysed by using several cephamycins, moxalactam, temocillin and related antibiotics. these compounds behaved as poor substrates for beta-lactamase ii, with high km values and very low catalytic efficiencies. in the case of beta ... | 1991 | 1930129 |
| mechanism of acyl transfer by the class a serine beta-lactamase of streptomyces albus g. | optimization by energy minimization of stable complexes occurring along the pathway of hydrolysis of benzylpenicillin and cephalosporin c by the streptomyces albus g beta-lactamase has highlighted a proton shuttle that may explain the catalytic mechanism of the beta-lactamases of class a. five residues, s70, s130, n132, t235 and a237, are involved in ligand binding. the gamma-oh group of t235 and, in the case of benzylpenicillin, the gamma-oh group of s130 interact with the carboxylate group, on ... | 1991 | 1930139 |
| metabolism of nitric oxide in denitrifying pseudomonas aeruginosa and nitrate-respiring bacillus cereus. | suspensions of pseudomonas aeruginosa and bacillus cereus were continuously sparged with nitrogen to remove gaseous products of nitrate reduction. under these conditions, p. aeruginosa denitrified nitrate to 4% no, 47% n2o and 49% n2. b. cereus reduced nitrate to 94% nitrite, 2% no and 5% n2o. b. cereus was unable to reduce no or n2o as sole electron acceptor, whereas p. aeruginosa reduced no stoichiometrically to n2o when the n2o reductase was inhibited by acetylene or when the formed n2o was i ... | 1991 | 1936933 |
| kinetics of the hydrolysis of monodispersed and micellar phosphatidylcholines catalyzed by a phospholipase c from bacillus cereus. | the phosphatidylcholine-hydrolyzing phospholipase c, so-called "phospholipase c" (plc), was isolated from the culture of bacillus cereus strain iam 1208. the amino-acid composition and partial n-terminal sequence of the purified enzyme were in good agreement with those expected from the nucleotide sequence for a plc of strain atcc 10987 [johansen et al. (1988) gene 65, 293-304]. the chain-length dependence of kinetic parameters for the plc-catalyzed hydrolysis of monodispersed short-chain phosph ... | 1991 | 1939031 |
| metabolism of 17 beta-estradiol by oral streptococcus mutans, streptococcus sanguis, bacillus cereus and candida albicans. | the ability of oral isolates of streptococcus mutans, streptococcus sanguis, bacillus cereus and candida albicans to metabolize 17 beta-estradiol was analyzed by thin-layer chromatography using 4-14c-estradiol as the substrate. all test microorganisms metabolized 17 beta-estradiol. estrone was the main metabolic product, except for b. cereus, which converted 17 beta-estradiol mainly to unidentified, more polar metabolites. s. sanguis and b. cereus had the most active metabolism but s. mutans and ... | 1991 | 1945489 |
| purification and some characteristics of a calcium-binding protein from bacillus cereus spores. | a novel calcium-binding protein has been purified from the dormant spores of bacillus cereus t. purity of this protein was verified by sds-page and reversed-phase hplc. its calcium-binding ability was verified by a competitive calcium-binding assay using chelex-100 resin and 45ca autoradiography. the protein is heat-stable and is retained by hydrophobic matrices (phenyl-sepharose) in a calcium-dependent manner. sds-page and amino acid composition indicate the molecular mass of the protein to be ... | 1991 | 1955855 |
| residues of doxycycline and oxytetracycline in eggs after medication via drinking water to laying hens. | doxycycline (dotc) and oxytetracycline (otc) were dissolved in drinking water (0.5 g/l) and supplied to laying hens for 7 consecutive days. eggs laid were collected daily during and after medication, and the antibiotic concentrations in the yolk and albumin were determined by the cup-plate method with bacillus cereus var. mycoides atcc 11778. the concentrations of both antibiotics were increased in yolk day by day with the advance in medication, reached peaks 2 days after withdrawal and then dec ... | 1991 | 2015932 |
| effects of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products. | pork slurries inoculated with various test microorganisms were prepared and subjected to high hydrostatic pressure at 1000 to 6000 atm for 10 min at 25 degrees c to examine for the pressure effects on characteristics of the slurries and the inactivation of the microorganisms associated with meat and meat products. pressure treatment at higher than 3000 atm caused coagulation and discoloration of the pork slurries. harder and more white coagulants were obtained by increasing the pressure. pressur ... | 1991 | 2049285 |