Publications

ulcer risk factors: interactions between helicobacter pylori infection, nonsteroidal use, and age.	to evaluate the influence of helicobacter pylori, nonsteroidal anti-inflammatory drug (nsaid) use, tobacco and alcohol use, age, gender, ethnic group, and the indication for endoscopy on the frequency of gastric and duodenal ulcers in patients referred for upper endoscopy.	1993	8475935
pathogenicity of helicobacter pylori: a perspective.		1993	8478048
cloning and expression of a high-molecular-mass major antigen of helicobacter pylori: evidence of linkage to cytotoxin production.	a high-molecular-mass (120- to 128-kda) helicobacter pylori antigen has been associated with peptic ulcer disease. we created a bank of 40,000 random chromosomal fragments of h. pylori 84-183 by using lambda zapii. screening of this bank in escherichia coli xl1-blue with absorbed serum from an h. pylori-infected person permitted the isolation and purification of a clone with a 3.5-kb insert. subcloning of this insert (pmc3) permitted the expression of a recombinant h. pylori protein that had a m ...	1993	8478069
in-vitro activity of dirithromycin in comparison with other new and established macrolides.	improvements with regard to the in-vitro activity of new macrolides are marginal and apply mainly to haemophilus spp., moraxella catarrhalis and neisseria gonorrhoeae (e.g. azithromycin is two to eight times more active than erythromycin) and to non-enterococcal streptococci (e.g. clarithromycin is two to four times more active than erythromycin). the increase in activity against staphylococci is even less striking, being restricted to a few species and limited to clarithromycin (twice as active ...	1993	8478311
radiation dose estimates for the carbon-14-labeled urea breath test.	the use of the 14c-urea breath test for diagnosis of helicobacter pylori infection in gastric mucosa has gained widespread acceptance and utilization. in order to obtain regulatory approval for this procedure, new dose estimates were required. previous radiation dose equivalent estimates for males only were based upon data published in 1975 for bicarbonate metabolism. since that time, calculational techniques for dose estimation have been significantly improved and the organ masses of reference ...	1993	8478718
helicobacter pylori prevalence in acquired immunodeficiency syndrome.	helicobacter pylori is consistently reported with high prevalence in hiv-negative patients with chronic gastritis and active ulcer disease. this study is an evaluation of the prevalence of h. pylori in aids patients, and the association with chronic gastritis, erosions, and ulcer disease. seventy-three aids patients referred for the evaluation of gastrointestinal symptoms underwent upper endoscopy and antral gastric biopsy. histologic gastritis was diagnosed and degree of activity graded on hema ...	1993	8480733
successful helicobacter pylori eradication: a systemic effect of antibiotics?		1993	8480762
helicobacter pylori.		1993	8481148
local cellular and immune response by antral mucosa in patients undergoing treatment for eradication of helicobacter pylori.	thirty-six patients with nonhealing or recurrent duodenal ulcers (du) were treated with omeprazole; 20 mg/day for one month followed by triple therapies (metronidazole, 400 mg three times a day, tetracycline, 500 mg four times a day with either colloidal bismuth, 120 mg four times a day or sucralfate 1 g four times a day. at least two gastric mucosal samples were collected from the antral portion of the stomach and from the duodenum before and immediately after omeprazole therapy and four weeks ...	1993	8482195
ethnic differences in helicobacter pylori infection.		1993	8482295
menetrier's disease: a form of hypertrophic gastropathy or gastritis?	menetrier's disease is characterized by giant gastric folds and foveolar hyperplasia. the extent of mucosal inflammation and glandular atrophy, however, is controversial. the aim of this study was to test the hypothesis that patients previously diagnosed with menetrier's disease actually represent at least two diseases: (1) hypertrophic lymphocytic gastritis (hlg) and (2) massive foveolar hyperplasia and minimal inflammation (mfh).	1993	8482445
two-week eradication regimen for metronidazole-resistant helicobacter pylori.	at present there is no generally accepted treatment regimen for eradicating metronidazole-resistant helicobacter pylori. this study determines the eradication rate after treatment with 40 mg omeprazole o.m. and 500 mg amoxycillin q.d.s. for 14 days, with 120 mg tripotassium dicitrato bismuthate q.d.s. for the first week (days 1-7) and 750 mg ciprofloxacin b.d. for the second week (days 8-14). thirty patients (16 male, mean age 45 years, range 16-80 years) with duodenal ulcers (n = 18) or non-ulc ...	1993	8485267
[antigens for the elisa test for serodiagnosis of helicobacter pylori infection].	semipurified urease and outer membranes of helicobacter pylori were isolated and used as antigens for the construction of new elisa tests for the serodiagnosis of infections caused by this microorganism. the highest discriminating capacity between infected and not infected subjects was obtained by the test detection of igg antibodies against the urease antigen which reached a 100% sensitivity, 90% specificity, a 100% negative and 91% positive predictive value. examination of sera of healthy chil ...	1993	8485766
[igg antibodies to gastrospirillum hominis and helicobacter pylori].	in conjunction with an investigation extending over several years, focused on the understanding of the importance of helicobacter pylori in inflammatory gastric disease, the authors examined a group of 1242 patients. in fifteen they detected by microscopic examination the presence of helical bacteria described in the literature as gastrospirillum hominis. the cultivation test was not positive. in three patients in addition to these bacteria also the presence of helicobacter pylori was detected. ...	1993	8485767
[a study on acid resistance of helicobacter pylori].	physiological saline solutions, each adjusted to ph 1.0, ph 2.0 and ph 7.2, and added urea and urease inhibitor (acetohydroxamic acid), were inoculated with a culture solution of helicobacter pylori nctc11637 strain derived from man, and allowed to react for 10, 30, 60 and 120 minutes. after five days of incubation on a blood agar medium, remaining viable h. pylori was counted. survival of h. pylori was inadequate under the conditions of ph 1.0 and ph 2.0, but survival up to 120 min was observed ...	1993	8486976
ultrastructure and biochemical studies of the flagellar sheath of helicobacter pylori.	helicobacter pylori flagellar sheaths were isolated by sucrose density-gradient centrifugation and analysed by electronmicroscopy, sds-page and gas-liquid chromatography. electronmicroscopy of thin sections of flagella showed an internal electron-dense filament and a surrounding flagellar sheath with the typical bilayer structure of a membrane. the flagellar filaments could be disintegrated by acid treatment and the resulting isolated flagellar sheaths formed vesicles, sometimes with characteris ...	1993	8487294
lethal nature of ischemic gastropathy.	gastropathy on the basis of mesenteric arterial ischemia can be masked in presentation as the typically more benign entities of gastritis, gastric ulceration, or gastric atony. gastritis and ulceration are commonly associated with stress, hyperacidity, helicobacter pylori infection, or medication injury. gastric atony is less commonly seen and usually attributable to diabetes mellitus, vagotomy, or mechanical gastric outlet obstruction. gastric ischemia as a cause of gastropathy is an underappre ...	1993	8488953
use of autoradiography to assess viability of helicobacter pylori in water.	autoradiographic methods have been developed to detect metabolic activity of viable but nonculturable cells of helicobacter pylori in water. four strains of h. pylori were studied by using microcosms containing suspensions of 72-h cultures in water. the suspensions of aged, nonculturable cells of h. pylori were incubated with [3h]thymidine for 24 to 72 h, after which the cell suspensions were exposed to kodak ntb2 emulsion for 3 to 28 days. each sample was processed with three separate controls ...	1993	8489232
proton pump inhibitors, enterochromaffin-like cell growth and helicobacter pylori gastritis.	in both rodents and humans the development of gastrin-promoted gastric argyrophil enterochromaffin-like cell carcinoids requires the involvement of a genetic factor inherent to multiple endocrine neoplasia syndrome or of type a autoimmune chronic atrophic gastritis. prolonged severe hypergastrinaemia acting on non-gastritic mucosa, as in zollinger-ellison syndrome patients, results in diffuse argyrophil enterochromaffin-like cell hyperplasia but, as a rule, does not produce tumours. combination ...	1993	8490076
future prospects for proton pump inhibitors.	future prospects for proton pump inhibitors depend on their efficacy and safety relative to h2-receptor antagonists, the therapeutic standard for acid peptic disorders. as safety concerns diminish, efficacy considerations become more important as these may pertain to cost effectiveness. comparative, controlled trials show that omeprazole and lansoprazole are somewhat more effective than h2-blockers in healing duodenal and gastric ulcers, providing faster relief of symptoms. relapse after cessati ...	1993	8490082
characteristics of the gastric mucosa of direct relatives of patients with sporadic gastric carcinoma.	we compared the gastric mucosa lesions of 63 direct relatives of 24 patients with gastric carcinoma with those observed in 151 dyspeptic patients and in seven members of a family with familial gastric carcinoma of diffuse type. we found a higher prevalence of helicobacter pylori infection (96.8%) and gastritis (98.4%) in the relatives of patients with sporadic carcinoma than in the dyspeptic patients. one-third of the individuals of the former group displayed chronic atrophic gastritis and intes ...	1993	8490543
gastric screening prospects.	the incidence of gastric cancer is rapidly declining in the western world, but it remains high in the third world and in japan. systematic screening for gastric cancer has been undertaken in japan, where barium x-ray is used in people over the age of 40. evaluation data suggest a benefit in reduced mortality, but biases cannot be ruled out. a similar screening programme has been started in venezuela. currently, stomach cancer screening programmes cannot be recommended as public health policy, ex ...	1993	8490548
serological diagnosis of helicobacter pylori--evaluation of four tests in the presence or absence of non-steroidal anti-inflammatory drugs.	the host's humoral immune response to helicobacter pylori has been used in the diagnosis of active infection with these organisms. several commercial tests are available but there are few and unconfirmed reports of their efficacy. this study aimed to assess and compare the efficacy of the following h pylori serological tests in patients treated or not treated with non-steroidal anti-inflammatory drugs (nsaid): pyloriset latex, helico-g, biolab malakit, and bio-rad gap test igg. venous blood was ...	1993	8491390
short and long term outcome of helicobacter pylori positive resistant duodenal ulcers treated with colloidal bismuth subcitrate plus antibiotics or sucralfate alone.	thirty two patients with helicobacter pylori positive duodenal ulcers resistant to treatment were randomly assigned to 4 weeks' treatment with sucralphate 4 g/day or colloidal bismuth subcitrate 480 mg/day plus amoxycillin from days 1 to 7 and tinidazole from days 8 to 14. after 4 weeks, patients with unhealed ulcers were crossed over to the other form of treatment for a further 4 week period. patients with healed ulcers were followed up for 1 year without maintenance therapy with clinical and e ...	1993	8491391
[helicobacter pylori and gastric mucosa].		1993	8492035
gastric heterotopic mucosa in the rectum with helicobacter pylori-like organisms: a rare cause of rectal bleeding.	we report the case of a 9-year-old boy who presented with chronic rectal bleeding. on proctosigmoidoscopy, a polypoid elevation in the rectal ampulla measuring 5 x 3 cm was found. biopsies were reported to show gastric mucosa. the giemsa stain showed helicobacter pylori-like organisms. including our patient, there are now 30 similar cases reported in the medical literature. the usual manifestations of this rare entity are chronic bleeding and rectal pain. in one-half of cases there is chronic re ...	1993	8492046
serodiagnosis of helicobacter pylori infection in children by an indirect immunofluorescence test.	the objective of this study was to evaluate the accuracy of an indirect immunofluorescence (iif) test for serodiagnosis of helicobacter pylori infection in children and to determine how the test is affected by the presence of antibodies against campylobacter jejuni. we studied 65 consecutive children (two with endoscopically confirmed duodenal ulcer) and a series of 18 children with duodenal ulcer. thirty children were h. pylori negative, as determined by culture, by the preformed urease test, a ...	1993	8492250
the prevalence of helicobacter pylori-positive serology in asymptomatic children.	epidemiologic data are presented about the prevalence of helicobacter pylori positivity as determined by serology in a large series of asymptomatic children (n = 466). serology is now generally accepted as a valid noninvasive screening method for the detection of helicobacter pylori infection. blood samples were taken from 466 apparently healthy children (ages 2-14 years) who were admitted to our pediatric 1-day clinic for elective surgery. the children originated from different ethnic backgroun ...	1993	8492251
upper gastrointestinal endoscopy in recurrent abdominal pain of childhood.	over a 2.5-year period, 82 consecutive children complaining of recurrent abdominal pain underwent upper gastrointestinal endoscopy. gastroscopy confirmed pathology in 48 of the children (58.5%). four of the children, who also had undergone gastroscopy, had other diagnoses (lactose malabsorption, hydronephrosis, yersiniosis), and 30 of the children (36.6%) retained the initial diagnosis of recurrent abdominal pain syndrome. gastritis was found in 48 of the children, 18 of whom (37.5%) had positiv ...	1993	8492255
[investigation of gastric function and prevalence of helicobacter pylori in non-ulcer dyspepsia].	one hundred and fourty four patients with non-ulcer dyspepsia (nud), as defined by the working party of aga in 1987, (67 men and 77 women, 16-76 years, mean age 42.9 +/- 1.2 years) and 34 asymptomatic controls (25 men and 9 women, 17-75 years, mean age 50.6 +/- 2.4 years) parameters of gastrophysiological function (gastric acid secretion, postprandial gastric emptying-acetaminophen method, serum gastrin levels and cutaneous electrogastrography (egg)) and the prevalence of helicobacter pylori (hp ...	1993	8492467
potent inhibitory action of the gastric proton pump inhibitor lansoprazole against urease activity of helicobacter pylori: unique action selective for h. pylori cells.	the gastric proton pump inhibitor lansoprazole, its active analog ag-2000, and omeprazole dose dependently inhibited urease activity extracted with distilled water from helicobacter pylori cells; the 50% inhibitory concentrations were between 3.6 and 9.5 microm, which were more potent than those of urease inhibitors, such as acetohydroxamic acid, hydroxyurea, and thiourea. these compounds also inhibited urease activity in intact cells of h. pylori and helicobacter mustelae but did not inhibit ur ...	1993	8494373
the phylogenetic position of helicobacter nemestrinae.	comparison of the helicobacter nemestrinae 16s ribosomal dna with published homologous sequences from members of the genera helicobacter, wolinella, and campylobacter reveals a close relationship between h. nemestrinae, h. pylori, and h. acinonyx. this finding is unexpected since these species differ significantly in their dna guanine-plus-cytosine contents (24 to 38 mol%).	1993	8494747
evaluation of quickvue, a rapid enzyme immunoassay test for the detection of serum antibodies to helicobacter pylori.	quickvue is an enzyme immunoassay test for qualitative detection of serum immunoglobulin-g antibodies to helicobacter pylori. we evaluated its ability to predict infection by h. pylori in 100 adult and 49 pediatric patients referred for gastric endoscopy. a patient was defined as infected with h. pylori if either culture or histology was positive. of the 100 adult patients, 64 had h. pylori infection and quickvue correctly identified 59 of the 64. of 36 h. pylori-negative patients, 20 were corre ...	1993	8495587
a human cdna corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins.	a clone, designated pag, was isolated by differential screening of cdna libraries made from the untransformed and ras-transformed human mammary epithelial cell line hbl100. this cdna corresponds to a gene constitutively expressed in most human cells which is induced to higher levels upon serum stimulation in untransformed and ras-transformed hbl100 cells. however, the abundance of the pag transcript is approximately 3-fold higher in transformed as compared to untransformed cells after 7-15 h of ...	1993	8496166
antibacterial action of the urease inhibitor acetohydroxamic acid on helicobacter pylori.	the urease inhibitor acetohydroxamic acid (aha) was assessed for its bacteriostatic and bactericidal effects on helicobacter pylori. for eight isolates of h pylori, the minimum inhibitory concentration (mic) was either 200 mg/l or 400 mg/l. interactions between aha and antimicrobial drugs used to treat h pylori were also determined. for most isolates aha reduced the mic for colloidal bismuth subcitrate (cbs), tetracycline, metronidazole, and amoxicillin. in a few isolates, however, aha increased ...	1993	8496397
helicobacter pylori infection in adolescents with eating disorders and dyspeptic symptoms.	the potential role of helicobacter pylori infection of the antrum as a cause of symptoms of dyspepsia in patients with eating disorders was evaluated with an assay to detect h. pylori-specific igg in serum. h. pylori-specific optical-density readings were comparable for adolescents with eating disorders (0.133 +/- 0.063, mean +/- sd) and for two comparison groups, and were lower than readings for children with documented h. pylori infection. symptoms referable to the gastrointestinal tract did n ...	1993	8496771
[helicobacter pylori infection and duodeno-gastric reflux].	we have studied and measured the duodenogastric reflux in 11 children with chronic abdominal pain and helicobacter pylori (hp) through 24-hours gastric ph-metric studies. all parameters evaluated: number of reflux episodes (nr 92.87 +/- 27.66), number of reflux episodes longer than 5.0 minutes (nr > 5m 11.45 +/- 4.68), longest reflux episode (lr 67.37 +/- 45.57) and alkaline reflux area (aa 604.89 +/- 443.54) had significative differences against a normal group (40 children). we concluded that d ...	1993	8499232
immunological activity of lipopolysaccharide of helicobacter pylori on human peripheral mononuclear blood cells in comparison to lipopolysaccharides of other intestinal bacteria.	lipopolysaccharide of helicobacter pylori was tested for its mitogenicity and for its ability to stimulate cytokine release in human peripheral blood mononuclear cells (pbmc) of healthy and h. pylori-infected blood donors. mitogenicity in pbmc induced by h. pylori lps was similar to that induced by campylobacter jejuni lipopolysaccharide, but lower than that induced by escherichia coli lipopolysaccharide in the h. pylori negative blood donor group. furthermore, h. pylori lps was able to induce t ...	1993	8499895
cell-associated haemolytic activity of helicobacter pylori.	helicobacter pylori cells cultured on solid medium were quantitatively tested for haemolytic activity against erythrocytes of man, sheep, the guinea pig and rabbit. using 4-day and 8-day cultures of two standard strains (atcc 43504, immi 676), human erythrocytes were not lysed by 10% bacterial suspensions. rabbit erythrocytes were the most sensitive to 8-day cultures. hot-cold incubation yielded the highest haemolysis titres. the extent of haemolysis strongly correlated with the number of bacter ...	1993	8500489
[validation and clinical and epidemiological use of a serologic test recommended in the diagnosis of helicobacter pylori infection].	a commercial serologic test using purified antigens of helicobacter pylori has been evaluated in the diagnosis of this infection. in a series of 250 patients undergoing endoscopy with antral biopsies for cytology, histology and culture, serology was positive in 68 of 71 patients with a positive culture (sensitivity: 96%) and negative in 67 of 69 patients with a normal mucosa and no microorganisms on biopsy (specificity: 97%). in the entire series, serology was positive in 33 patients with no inf ...	1993	8500715
antral g-cell and d-cell numbers in helicobacter pylori infection: effect of h. pylori eradication.	it has recently been recognized that helicobacter pylori infection is associated with abnormalities in the regulation of gastrin secretion. we investigated whether there was a relationship between h. pylori infection and g-cell and d-cell numbers.	1993	8500723
animal models for helicobacter pylori.		1993	8500753
receptor affinity purification of a lipid-binding adhesin from helicobacter pylori.	our previous work has shown that helicobacter pylori specifically recognizes gangliotetraosylceramide, gangliotriaosylceramide, and phosphatidylethanolamine in vitro. this binding specificity is shared by exoenzyme s from pseudomonas aeruginosa, and monoclonal antibodies against this adhesin prevent the attachment of h. pylori to its lipid receptors. we now report the use of a novel, versatile affinity matrix to purify a 63-kda exoenzyme s-like adhesin from h. pylori which is responsible for the ...	1993	8500882
expression of catalytically active recombinant helicobacter pylori urease at wild-type levels in escherichia coli.	the genes encoding helicobacter pylori urease, a nickel metalloenzyme, have been cloned and expressed in escherichia coli. enzymatic activity, however, has been very weak compared with that in clinical isolates of h. pylori. conditions under which near wild-type urease activity was achieved were developed. e. coli. se5000 containing recombinant h. pylori urease genes was grown in minimal medium containing no amino acids, nicl2 was added to 0.75 microm, and structural genes urea and ureb (php902) ...	1993	8500893
helicobacter mustelae and helicobacter pylori bind to common lipid receptors in vitro.	helicobacter pylori is a recently recognized human pathogen causing chronic-active gastritis in association with duodenal ulcers and gastric cancer. helicobacter mustelae is a closely related bacterium with similar biochemical and morphologic characteristics. h. mustelae infection of antral and fundic mucosa in adult ferrets causes chronic gastritis. an essential virulence property of both helicobacter species is bacterial adhesion to mucosal surfaces. the aim of this study was to determine whet ...	1993	8500901
iron acquisition by helicobacter pylori: importance of human lactoferrin.	helicobacter pylori is known to be an etiologic agent of gastritis and peptic ulcer disease in humans. however, the mechanism by which this organism acquires iron has not been studied. for this investigation, h. pylori was grown in iron-restricted medium. siderophore production was not detected by chemical assays, and the strains were unable to use enterochelin and pyochelin for growth in low-iron media. human lactoferrin supported full growth of the bacteria in media lacking other iron sources, ...	1993	8500909
cloning and genetic characterization of the helicobacter pylori and helicobacter mustelae flab flagellin genes and construction of h. pylori flaa- and flab-negative mutants by electroporation-mediated allelic exchange.	helicobacter pylori is one of the most common human pathogens. it causes chronic gastritis and is involved in the pathogenesis of gastroduodenal ulcer disease and possibly gastric carcinoma. helicobacter mustelae is a bacterium closely related to h. pylori that causes gastritis and ulcer disease in ferrets and is therefore considered an important animal model of gastric helicobacter infections. motility, even in a viscous environment, is conferred to the bacteria by several sheathed flagella and ...	1993	8501031
helicobacter pylori in children with acquired immunodeficiency syndrome.		1993	8502537
helicobacter pylori infection in elderly people: correlation between histology and serology.	a hundred elderly dyspeptic patients were studied to assess the prevalence of helicobacter pylori infection and the correlation between histological and serological findings. eighty-one per cent of the patients with gastritis and 63% with gastric ulcer were h. pylori positive. all patients who had h. pylori negative gastritis and gastric ulcers were on nonsteroidal anti-inflammatory drugs (nsaids). there were 24 patients who had evidence of h. pylori infection and were on nsaids. h. pylori posit ...	1993	8503319
helicobacter pylori and non-steroidal anti-inflammatory drugs: uncomfortable partners in peptic ulcer disease.		1993	8504954
gastric mucosal barrier: evidence for helicobacter pylori ingesting gastric surfactant and deriving protection from it.	ultrastructural examination by electron microscopy has been undertaken on human oxyntic mucosa from biopsy specimens obtained during diagnostic endoscopy from patients in whom infection by helicobacter pylori was subsequently confirmed. a novel fixation procedure was used that avoided conventional fixatives based upon glutaraldehyde, which can destroy the hydrophobic lining of surfaces such as gastric mucosa. the resulting electron micrographs show densely osmiophilic inclusions of varying sizes ...	1993	8504956
13c-urea breath test for diagnosis of experimental helicobacter pylori infection in barrier born pigs.	previous studies with helicobacter pylori infected barrier born pigs indicate that the infection has a patchy distribution, resulting in false negative culture results on endoscopic biopsy specimens. this study aimed to adapt the 13c-urea breath test as used in humans to diagnose h pylori infection in barrier born pigs. the breath test was also performed after bismuth as a single treatment and after triple therapy (bismuth, ampicillin, metronidazole). in control pigs the median excess of 13co2 i ...	1993	8504957
incidence of helicobacter pylori strains activating neutrophils in patients with peptic ulcer disease.	a total of 61 human gastric isolates of helicobacter pylori were studied for their ability to induce an oxidative burst in human neutrophils measured by luminol enhanced chemiluminescence. about one third of the strains induced strong and rapid chemiluminescence in neutrophils even without serum opsonins and agglutinated these cells on glass slides within two minutes. for other strains complement was required, although even then the reactions remained at a lower level. the activating and aggluti ...	1993	8504958
gastric lymphoid follicles in helicobacter pylori infection: frequency, distribution, and response to triple therapy.	to determine the prevalence and distribution of gastric lymphoid follicles in patients with helicobacter pylori infection, to evaluate their relationship with gastroduodenal pathology, and to assess their evolution after eradication of h pylori, mapped gastric biopsy specimens were obtained from 20 h pylori-negative normal volunteers, 25 asymptomatic volunteers with h pylori infection and no ulcer disease, 21 duodenal ulcer patients, and 16 patients with gastric ulcer. nine infected subjects wer ...	1993	8505036
helicobacter pylori infection in greece in healthy people and in patients with peptic ulcer and with dyspepsia without ulcer.		1993	8505502
ménétrier's disease and helicobacter pylori.		1993	8505952
[therapy of peptic ulcer].	after a historical review of therapeutic methods of peptic ulcer the author discusses contemporary views on therapy. as a basis he uses the idea that helicobacter pylori is the decisive factor for the development of peptic ulcers. therefore the basis of treatment should be its eradication by bismuth salts. despite this at present the basis of treatment are antagonists of h2 receptors. economic aspects are important. in repeatedly relapsing ulcers with evidence of helicobacter pylori the author r ...	1993	8506657
[helicobacter pylori in the etiology of ulcer disease and gastritis].	helicobacter pylori (hp) is unequivocally the most frequent cause of antral gastritis and most probably participates in the majority of cases in the aetiology of gastritis affecting the body of the stomach. the close relationship between antral gastritis and duodenal ulceration is documented by the high incidence of hp in these patients (90-100%). the problem of hp negative cases of duodenal ulceration was not finally resolved yet. the absence of hp in duodenal ulcer usually implies a different ...	1993	8506675
[role of helicobacter pylori in peptic ulcers].		1993	8507802
inhibition of helicobacter pylori colonization by an antiulcer agent, sulglycotide.	sulglycotide, a potent antiulcer agent derived from duodenal mucus glycopeptide through sulfation of the carbohydrate moieties, was evaluated with respect to its ability to interfere with h. pylori mucosal attachment. h. pylori cells were incubated with sulglycotide or human gastric mucin and then examined for their inhibitory capacity of h. pylori attachment to erythrocytes. titration data revealed that the mucin inhibitory activity was confined to its sulfomucin fraction, the titer of which wa ...	1993	8508147
dna-dna hybridization demonstrates apparent genetic differences between helicobacter pylori from patients with duodenal ulcer and asymptomatic gastritis.	we asked whether different clinical outcomes of helicobacter pylori infection might be a reflection of genetic differences in infecting organisms. using dna-dna hybridization we examined whether hybridization levels grouped h. pylori isolates corresponding to the type of disease (gastric ulcer, duodenal ulcer, asymptomatic gastritis) from which they were recovered. target dnas were prepared from h. pylori strains cultured from gastric biopsy specimens of 25 patients; 5 with gastric ulcers, 9 wit ...	1993	8508708
prospective, randomized, investigator-blind trial of helicobacter pylori infection treatment in patients with refractory duodenal ulcers. healing and long-term relapse rates.	in this study, 26 patients with duodenal ulcers refractory to treatment with h2-receptor antagonists for 8-12 weeks were randomly assigned to eight weeks of treatment with colloidal bismuth subcitrate (120 mg four times a day) alone (n = 12) or in combination with tetracycline hydrochloride (500 mg four times a day, days 0-14) and metronidazole (500 mg three times a day, days 15-28). symptoms were scored and endoscopy, histology, and clo tests were performed before, on completion of treatment, a ...	1993	8508709
helicobacter pylori serology in patients with gastric carcinoma.	helicobacter pylori-associated gastritis has been put forward as a distinct risk factor for gastric cancer. furthermore, among h. pylori-positive individuals a correlation between a high serum level of h. pylori antibodies and the risk of gastric cancer has been found in two different studies. other studies have challenged this hypothesis. we therefore studied the presence and level of h. pylori serum antibodies, using an enzyme-linked immunosorbent assay technique in 116 gastric cancer patients ...	1993	8511504
prevention of duodenal ulcer recurrence with penicillin. a double-blind, placebo-controlled trial.	eradication of helicobacter pylori is associated with a reduced recurrence of duodenal ulcer (du). the relationship between h. pylori and du has been interpreted as causal, but the evidence has been criticized for methodologic reasons. to ascertain whether an antibiotic with no effect on epithelial-cell integrity prevents du recurrence, we conducted a randomized double-blind trial of phenoxymethylpenicillin (pen), 2.4 twice daily, and placebo (pla). patients with an active du and positive h. pyl ...	1993	8511505
helicobacter pylori in 205 consecutive endoscopy patients.	the presence of helicobacter pylori in the gastric, antral mucosa of 205 consecutive, unselected gastroscopy patients was investigated by 1-3 biopsies for urea broth test and culture, 1 biopsy for histological examination and 1 blood sample for serology by elisa. overall, 41% were positive for h. pylori by culture, 32% by urea broth test, 24% by histological staining and 67%, 56% and 49% for the 3 cut-off limits applied to serology. culture and serology indicated the presence of h. pylori in 79- ...	1993	8511512
[iga-antibodies against helicobacter pylori in gastric secretions: gastric secretory immune response or salivary contamination?].	iga anti-hp concentrations in both gastric juice and saliva samples of 40 hp+ patients (15 f, 25 m) randomly referred for upper gi-endoscopy were measured. the presence of hp was investigated using histology (h&e and giemsa stain), rapid urease test (clo) and serology (cobas core anti-h. pylori eia, f. hoffmann-la roche). iga anti-hp in gastric juice and saliva was determined by elisa; the same hp antigen was used as for serology (fplc-purified native cell surface antigens including urease, free ...	1993	8511543
helicobacter pylori prevalence in non-ulcer dyspepsia--ethnic and socio-economic differences.	helicobacter pylori is an important cause of gastritis and a number of therapeutic trials suggest that it may be important in the genesis of duodenal ulcer recurrence. the reported prevalence of gastric colonisation by the organism varies considerably. the aim of this cross-sectional survey was to determine its prevalence in non-ulcer dyspeptics and to determine whether this is influenced by age, race, sex, socio-economic status, educational level and the number of persons sharing accommodation. ...	1993	8511682
evaluation of helicobacter pylori sensitivity to amoxycillin and metronidazole in dyspeptic patients.	we evaluated the "in vitro" sensitivity to amoxycillin and metronidazole of 193 helicobacter pylori strains isolated from the gastric mucosa of dyspeptic patients. susceptibility was determined by disc diffusion on agar plates. all the isolates were found to be sensitive to amoxycillin. on the contrary, 12% of the strains isolated from patients never treated for helicobacter pylori infection and 73% of those isolated from patients who had previously received unsuccessful treatment for helicobact ...	1993	8513165
absence of helicobacter pylori in dental plaques in helicobacter pylori positive dyspeptic patients.	to test the validity of reports on detection of helicobacter pylori in the mouth, samples were obtained simultaneously from the gastric mucosa and dental plaques for culture in 94 patients examined consecutively by endoscopy. histological examinations and serological tests were also performed. helicobacter pylori was not found in the mouth of any of the patients including 52 who had culture-positive gastric biopsies. thus earlier results could not be confirmed, however, other techniques such as ...	1993	8513816
enhanced h+ diffusion by nh4+/hco3-: implications for helicobacter-pylori-associated peptic ulceration.	the in vitro effect of ammonium bicarbonate buffer on mucus h+ permeability is reported here. the diffusional resistance of mucus and water was demonstrated to be dependent on buffer concentration, and the contrast between the two types of layers was most pronounced for low buffer concentration near neutrality. moreover, the pka values of hco3- and nh3 had a profound effect on measured dhcl. these in vitro studies suggest that a potentially damaging high local concentration of nh3 and hco3- with ...	1993	8513985
helicobacter pylori: current status.		1993	8514046
contact of lymphocytes with helicobacter pylori augments natural killer cell activity and induces production of gamma interferon.	we studied the capacity of glutaraldehyde-fixed helicobacter pylori to stimulate natural killer (nk) cell activity. bacteria were incubated overnight with peripheral blood lymphocytes enriched for large granular lymphocytes (lgl), the mediators of non-major histocompatibility complex-restricted cellular cytotoxicity. then, the cytolytic activity of lgl was tested against various tumor target cells. we observed that efficient cytolytic activity was generated against resistant and nonresistant tum ...	1993	8514408
the cohort effect and helicobacter pylori.	a total of 631 serum samples collected in 1969, 1979, and 1989 from adults and children were screened for helicobacter pylori by western blot analysis. results showed that h. pylori seroprevalence has become less frequent over the 20-year period. by studying seropositivity by year of birth, the magnitude of a cohort effect of h. pylori seropositivity was estimated. the odds of being seropositive decreased by 26% per decade, p = .008 (95% confidence interval, 8%-41%). estimates of seroprevalence ...	1993	8515114
seroprevalence of helicobacter pylori in chile: vegetables may serve as one route of transmission.	helicobacter pylori infection is very common in chilean adults, but the age-related prevalence, risk factors for infection, and mode of transmission in chilean children are unknown. an elisa was used to test for h. pylori antibodies in 1815 chileans < 35 years of age. seropositivity was > 60% in lower socioeconomic groups. h. pylori seropositivity correlated with increased age, low socioeconomic status, and consumption of uncooked vegetables by use of a logistic regression analysis. risk factors ...	1993	8515115
molecular characterization of the 128-kda immunodominant antigen of helicobacter pylori associated with cytotoxicity and duodenal ulcer.	helicobacter pylori has been associated with gastritis, peptic ulcer, and gastric adenocarcinoma. we report the nucleotide sequence and expression of an immunodominant antigen of h. pylori and the immune response to the antigen during disease. the antigen, named caga (cytotoxin-associated gene a), is a hydrophilic, surface-exposed protein of 128 kda produced by most clinical isolates. the size of the caga gene and its protein varies in different strains by a mechanism that involves duplication o ...	1993	8516329
in vitro susceptibility of helicobacter pylori to several antimicrobial combinations.	synergy between metronidazole and its hydroxymetabolite and between each compound and amoxicillin or tetracycline-hcl was determined against helicobacter pylori. metronidazole plus its hydroxymetabolite and either compound combined with amoxicillin showed synergism against all 10 strains of h. pylori tested. metronidazole plus tetracycline-hcl or the hydroxymetabolite plus tetracycline-hcl acted synergistically against seven and six strains, respectively, acted additively against three strains, ...	1993	8517712
helicobacter pylori and gastric cancer.	this review focuses on the similarities between the epidemiology of gastric cancer and the epidemiology of helicobacter pylori. their demographic patterns and the results of studies regarding familial and environmental risk factors are described. the association of gastric cancer and h. pylori infection with both gastric ulcer and chronic atrophic gastritis is also characterized and the possibility that a h. pylori infection could lead to gastric cancer is discussed.	1993	8518402
helicobacter pylori toxin inhibits growth and proliferation of cultured gastric cells-kato iii.	the presence of hp is strongly associated with chronic type b gastritis and peptic ulcer disease. the pathogenic mechanisms of helicobacter pylori (hp) infection are still unclear. hp produces toxins which are capable of exerting cytotoxic effects. whether these changes result in decreased cell proliferation has not been previously demonstrated. our results show that a 2 hour incubation of gastric kato iii cells with hp cytotoxin produces a 60% decrease in cell proliferation. in conjunction with ...	1993	8518421
glycosulfatase activity of helicobacter pylori towards gastric sulfomucin: effect of nitecapone.	a glycosulfatase activity towards human gastric sulfomucin was identified in the extracellular material elaborated by h. pylori, a pathogen implicated in the etiology of gastric disease. the purified enzyme exhibited maximum activity at ph 5.7 in the presence of 0.3% triton x-100 and 100mm cacl2, and displayed on sds-page an apparent molecular weight of 30kda. the h. pylori glycosulfatase effectively caused desulfation of n-acetylglucosamine-6-sulfate and galactose-6-sulfate of the carbohydrate ...	1993	8518427
[significance of helicobacter pylori infection in the pathogenesis and natural history of peptic ulcer].	recent studies strongly suggest that helicobacter pylori might have a significant role in the pathogenesis and rate of recurrence of peptic duodenal ulcer. various factors, such as the existence of ulcerogenic strains of the bacteria and susceptible subjects, might explain the fact that not more than 10% of the patients infected with h pylori actually have duodenal ulcer. its association with gastric ulcer and gastric carcinoma is not well defined.	1993	8235170
[helicobacter pylori: clinical pictures of an infectious disease with pathogen persistence].	it is the aim of this paper to provide survey of the very fast developing field of medical research on helicobacter pylori which includes as different branches as gastroenterology, pharmacology or microbiology. after examination of the several diseases, pathological mechanisms, the diagnostical techniques and the therapeutical regimens, the authors of this study favorize an early indication for eradication of helicobater pylori. since research on helicobacter pylori is divided into many fields, ...	1993	8237024
[the role of helicobacter pylori in development of ulcers].		1993	8237063
clarithromycin as monotherapy for eradication of helicobacter pylori: a randomized, double-blind trial.	current regimens to eradicate helicobacter pylori usually consist of metronidazole plus a bismuth compound, as well as a third agent such as tetracycline. such regimens are not ideal because organisms may be metronidazole-resistant, side-effects occur, and compliance is often poor. this randomized, double-blind study was designed to assess the ability of clarithromycin, a new macrolide antimicrobial, as monotherapy to eradicate h. pylori. thirty-seven healthy volunteers who were h. pylori positi ...	1993	8237933
minimum analysis requirements for the detection of helicobacter pylori infection by the 13c-urea breath test.	in an attempt to identify the minimum criteria for scoring the 13c-urea breath test, we examined test results from 96 healthy subjects, 270 duodenal ulcer patients undergoing treatment to eradicate helicobacter pylori, and 1000 consecutive breath tests analyzed in our laboratory.	1993	8237934
helicobacter pylori is a risk factor for hepatic encephalopathy in acute alcoholic hepatitis: the ammonia hypothesis revisited. the veterans administration cooperative study group no. 275.	to determine whether infection with helicobacter pylori is a risk factor for portosystemic encephalopathy in patients with acute, moderate or severe alcoholic hepatitis.	1993	8237940
acid inhibition and amoxicillin activity against helicobacter pylori.		1993	8237959
combined intravenous and oral omeprazole/amoxicillin therapy in patients with helicobacter pylori-positive acute peptic ulcer bleeding.		1993	8237963
evaluation of rapid urease test for detection of helicobacter pylori in gastric biopsy specimens.	using 80 gastric biopsy specimens from patients with various gastroduodenal diseases, the isolation rate of h. pylori and urease activity of the biopsy specimens were examined. the sensitivity and specificity of the rapid urease test was 84.6% and 75.9%, respectively, for the cultivation of h. pylori. the mean number of h. pylori detected in urease-positive and negative biopsy specimens was 10(6.1 +/- 0.9) and 10(4.5 +/- 1.2) cfu/g specimens, respectively.	1993	8243603
non-steroidal anti-inflammatory drug-induced gastropathy: a comparative endoscopic and histopathological evaluation of the effects of tenoxicam and diclofenac.	a 4-week double-blind study compared the potential for 20 mg/day tenoxicam or 100 mg/day diclofenac sodium to induce gastropathy in 36 patients with joint disease and assessed the influence of gastric colonization by helicobacter pylori. endoscopic assessment at the end of 4 weeks indicated that the mucosa was normal in 79% of tenoxicam-treated patients and 59% of diclofenac-treated patients. only 5% of patients in the tenoxicam group developed severe gastroduodenitis (> 11 haemorrhages or erosi ...	1993	8243794
diagnostic value of serum pepsinogen c in patients with raised serum concentrations of pepsinogen a.	hypopepsinogenaemia a is often found in patients with gastric atrophy and gastric surgery. in these conditions serum pepsinogen c provides additional diagnostic information, especially when expressed as pepsinogen a:c ratio. hyperpepsinogenaemia a has been shown in patients with duodenal ulcer disease, zollinger-ellison syndrome, hypertrophic gastropathy, chronic renal failure, and during omeprazole treatment. as patients with hyperpepsinogenaemia a often overlap in symptoms, endoscopical findin ...	1993	8244094
systemic and mucosal humoral responses to helicobacter pylori in gastric cancer.	the systemic igg response to helicobacter pylori was examined in 70 patients with gastric cancer. h pylori igg antibodies were assayed by enzyme linked immunosorbent assay (elisa), and serological recognition of h pylori antigens was characterised by western blotting. a percentage of 78.5 were seropositive by elisa. two of five patients under age 50 were seronegative. positivity was unrelated to age, sex, tumour type, or site. ninety one per cent of elisa positive cancer patients recognised the ...	1993	8244098
acute perforated duodenal ulcer is not associated with helicobacter pylori infection.	most patients with chronic duodenal ulcer disease have helicobacter pylori infection and eradicating it considerably reduces the relapse rate. the prevalence of h pylori in 80 patients (mean age = 52 years, range 17-85) presenting with acute perforated duodenal ulcer was examined and compared with age and sex matched hospital control patients. h pylori state was assessed by serum anti-h pylori igg (helico-g kit, porton) using a titre of 18 or less as negative with a specificity of 89% and sensit ...	1993	8244099
helicobacter pylori: comparison of dna fingerprints provides evidence for intrafamilial infection.	although a high prevalence of antibodies to helicobacter pylori has been documented within families, culture and dna typing of strains from infected children and their parents has not been evaluated. this study aimed to analyse h pylori infection within family groups. endoscopy, gastric biopsy, and h pylori culture were performed on all eight parents of four children who presented with dyspepsia and who had a positive h pylori culture. all biopsy specimens were cultured on columbia based blood a ...	1993	8244100
serological diagnosis of helicobacter pylori.		1993	8244124
ultrastructural patterns of helicobacter pylori.	ultrastructural morphology of the bacterial bodies was studied in 40 helicobacter pylori positive cases. two bacterial patterns were identified, which were associated with different modes of contact with the epithelial cells and possibly with different stages of the natural history of the infection.	1993	8244133
gastric metaplasia and helicobacter pylori infection.	duodenal and antral mucosal biopsy specimens were obtained from 139 patients with dyspeptic complaints to study the prevalence and extent of gastric metaplasia in the duodenal bulb in relation to helicobacter pylori (h pylori) infection and duodenal ulcer disease. on logistic regression, the presence and extent of gastric metaplasia was not significantly associated with h pylori infection. the prevalence of gastric metaplasia, however, was found to be higher in patients with current or past evid ...	1993	8244134
helicobacter pylori positive resistant duodenal ulcers.		1993	8244159
helicobacter pylori positive resistant duodenal ulcers.		1993	8244160
helicobacter pylori positive resistant duodenal ulcers.		1993	8244161
[helicobacter pylori associated gastritis: a primary study of ammonia and urea in gastric juice and mucus in gastric mucosa].	the mucosa of 46 patients with histological chronic gastritis were cultured for helicobacter pylori (hp) and the ammonia and urea in their gastric juice were also determined. the results showed the average ammonia concentration (1.22 +/- 0.23 mmol/l) in hp positive patients was higher than that of hp negative patients (0.72 +/- 0.25 mmol/l), (p < 0.05). but the average urea concentration (0.45 +/- 0.29 mmol/l) in hp positive patients was lower than that of hp negative patients (2.71 +/- 1.20 mmo ...	1993	8244308