Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| vasopressin stimulates the phosphorylation of an 83,000 mr protein in the superior cervical ganglion. | 1. 32p-labeled proteins from the superior cervical ganglion of the rat were separated by two-dimensional gel electrophoresis and visualized by autoradiography. 2. the most heavily labeled phosphoprotein in the ganglion had a relative molecular weight of 83,000 and a pi of 4.5. phosphorylation of this protein was increased by phorbol 12,13-dibutyrate, an activator of the ca2+/phospholipid-dependent protein kinase, protein kinase c. this protein appears to be similar or identical to a specific pro ... | 1987 | 3451798 |
| surface-active agents from two bacillus species. | two bacillus species were studied which produced bioemulsifiers; however, they were distinctly different compounds. bacillus sp. strain iaf 343 produced unusually high yields of extracellular biosurfactant when grown on a medium containing only water-soluble substrates. the yield of 1 g/liter was appreciably better than those of most of the biosurfactants reported previously. this neutral lipid product, unlike most lipid biosurfactants, had significant emulsifying properties. it did not apprecia ... | 1987 | 16347271 |
| isolation and characterization of a bacillus cereus mutant strain hyperproductive of exo-beta-amylase. | starting with a strain of bacillus cereus excreting about 40-fold more beta-amylase than does the original wild-type strain, we isolated, after mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine, a strain designated bq10-s1 spoiii which showed under optimal conditions a further 5.5-fold increase in beta-amylase activity. the amylase production of this strain was observed to increase in the presence of 0.5% glucose or 1% maltose and, more markedly, in the presence of 2% soluble starch in the c ... | 1987 | 16347320 |
| a new bioassay system for screening high berberine-producing cell colonies of thalictrum minus. | a new method of estimating the amount of berberine released from minute cell colonies of thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. in this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and the concentration of berberine which has been released from the cells into the agar piece is assayed by the antibacterial activity against bacillus cereus mt2026. screening of 1000 ... | 1987 | 24248650 |
| effects of lactic acid bacteria and organic acids on growth and germination of bacillus cereus. | growth and germination of vegetative cells and endospores of bacillus cereus were affected by streptococcus lactis, streptococcus thermophilus, lactobacillus acidophilus, and lactobacillus bulgaricus in nonfat milk medium and by salts of organic acids in broth medium. growth of the lactic acid bacteria was not affected by b. cereus. b. cereus increased rapidly to about 10 cfu/ml when cells were added at the beginning of growth of lactic acid bacteria; it was inactivated slowly when added after 2 ... | 1988 | 16347729 |
| the relationship between the structures of four beta-lactamases obtained from bacillus cereus. | bacillus cereus has proved to be one of the most interesting microorganisms in the study of beta-lactamases. it secrets these enzymes very efficiently and, frequently, in multiple forms. three different forms are produced by strain 569/h; mutant 5/b of the same microorganism is constitutive for the secretion of beta-lactamases i and ii. the present study, based on secondary structure prediction by two independent methods, states the relationship among the structures of beta-lactamases i, ii and ... | 1988 | 3155326 |
| the biosynthetic implications of acetate and glutamate incorporation into (3r,5r)-carbapenam-3-carboxylic acid and (5r)-carbapen-2-em-3-carboxylic acid by serratia sp. | two new beta-lactams have been isolated from strains of serratia and erwinia sp. and identified as (3r,5r)- and (3s,5r)-carbapenam-3-carboxylic acid. these novel carbapenams lack antibacterial activity, are resistant to both beta-lactamases i and ii from bacillus cereus and are not detected by the lactamase induction assay. radiolabelled and stable isotope experiments have established that both metabolites together with the antibiotic 5r-carbapenem-3-carboxylic acid are glutamate and acetate der ... | 1988 | 3182403 |
| bacillus species isolates from cerebrospinal fluid in patients without shunts. | of 849 csf cultures done at hartford hospital, nine were positive for nonanthrax bacillus species. differentiation of true nonanthrax bacillus species infection from contamination requires careful consideration of the clinical findings, the clinical course, and the laboratory data. in seven patients the nonanthrax bacillus species represented contamination. in two patients the nonanthrax bacillus species represented true infection. in one of these infected patients, nonanthrax bacillus species c ... | 1988 | 3186383 |
| five unique temperate phages from a polylysogenic strain of bacillus thuringiensis subsp. aizawai. | five temperate phages were isolated from strain 4042b of bacillus thuringiensis subsp. aizawai. the phages, which were heteroimmune, could also be distinguished by their host ranges, plaque and particle morphologies, serological specificities, and locations of restriction endonuclease cleavage sites on their chromosomes. besides maintaining a stable lysogenic relationship with the 4042b host strain, each phage formed a stable lysogen with bacillus cereus. | 1988 | 3221199 |
| microorganisms associated with mouldiness of dried yam chips and their prevention. | the broad objective of this study was to isolate and identify the microorganisms causing mouldiness of stored yam chips and to look for ways of preventing the problem. microorganisms isolated included aspergillus flavus, a. glaucus, a. nidulans, a. niger, a. ochraceous, a. tamarii, a. candidus, penicillium oxalicum, trichoderma longibrachyatum, rhizopus nigricans, cylindrocarpon radicicola, neurospora crassa, botryodiplodia theobromae, bacillus subtilis, bacillus cereus, erwinia carotovora and s ... | 1988 | 3231261 |
| on the kinetic interaction between ceftriaxone and some beta-lactamases. | the activity of beta-lactamases from citrobacter diversus ula-27 on ceftriaxone, a widely recognized third-generation cephalosporin, has been examined and compared to the activity of various other beta-lactamases from different sources. ceftriaxone (roche s.p.a. milan) was found to be resistant to hydrolysis by beta-lactamases from enterobacter cloacae and bacillus cereus, but susceptible to beta-lactamases from mycobacterium fortuitum strain cow 18 and, mostly, to beta-lactamases from various s ... | 1988 | 3260552 |
| imipenem as substrate and inhibitor of beta-lactamases. | the interaction between imipenem, a carbapenem antibiotic, and two representative beta-lactamases has been studied. the first enzyme was beta-lactamase i, a class-a beta-lactamase from bacillus cereus; imipenem behaved as a slow substrate (kcat. 6.7 min-1, km 0.4 mm at 30 degrees c and at ph 7) that reacted by a branched pathway. there was transient formation of an altered species formed in a reversible reaction; this species was probably an acyl-enzyme in a slightly altered, but considerably mo ... | 1988 | 3263117 |
| modified general affinity adsorbent for large-scale purification of penicillinases. | n-acetyl-d-(-)-penicillamine as a stable second-generation biospecific affinity ligand has previously been suggested for purification of bacillus cereus 569/h beta-lactamase i. a complex spacer arm is coupled with the matrix by using epichlorohydrin and phloroglucinol doubly activated with divinyl sulphone in the meta position. coupling of d-(-)-penicillamine ligand resulted in an active affigel. however, we found that two affinity ligands in close proximity prevents simultaneous binding of two ... | 1988 | 3265708 |
| study of the bacillus flora of nigerian spices. | bacteriological examination of 230 samples of five different unprocessed spices (aligator pepper, red pepper, black pepper, thyme and curry powder) collected randomly from port harcourt main markets revealed that the spices were highly contaminated, with bacterial counts ranging from 1.8 x 10(4) to 1.1 x 10(8) per gram. bacillus cereus was isolated in high numbers in the majority of the 230 samples examined. it was also observed that other bacillus spp. including b. subtilis, b. polymyxa and b. ... | 1988 | 3275301 |
| physiology of sporolactobacillus strains isolated from different habitats and the indication of in vitro antagonism against bacillus species. | in an ecological study only low numbers of sporolactobacillus were found in habitats such as the faeces of herbivores, the rumen of cattle and the final waste water of an abattoir. their presence in the final waste water of an abattoir indicates their possible association with food, and, more specifically, with meat. differences were found in some physiological characteristics. one isolate (l2404) differed from the authentic sporolactobacillus atcc 15538 by its inability to ferment inulin, its g ... | 1988 | 3275317 |
| effects of phospholipase c, a tissue thromboplastin inhibitor, on pulmonary microembolism after missile injury of the limb. | tissue thromboplastin probably plays an important role in the development of post-traumatic pulmonary microembolism. infusion of purified human tissue thromboplastin in animals resulted in an intravascular coagulation and respiratory insufficiency. this could be inhibited by previous infusion of phospholipase c (plc) from bacillus cereus. we have studied the effects of plc infusion on the course of post-traumatic pulmonary microembolism, induced by a high-energy (c. 700 j) missile trauma to the ... | 1988 | 3339692 |
| [surface changes of intravenous catheters after antineoplastic chemotherapy]. | during long-term venous catheter implantation, septic and thrombotic complications are quite frequent. in the case reported, the failure of systemic and local antibiotic therapy during repeated septicaemia due to bacillus cereus at the time of intensive chemotherapy led to a scanning electron microscopy study of the used silicone catheter. there were marked changes of the inner surface with a lot of cellular remains, in contrast with the usual non thrombogenic property of the silicone. an in vit ... | 1988 | 3348519 |
| in vitro susceptibility of bacillus spp. to selected antimicrobial agents. | although often dismissed as contaminants when isolated from blood cultures, bacillus spp. are increasingly recognized as capable of causing serious systemic infections. as part of a clinical-microbiological study, 89 strains of bacillus spp. isolated from clinical blood cultures between 1981 and 1985 had their species determined and were tested for antimicrobial agent susceptibility to 18 antibiotics. species of isolates were determined by the api 50ch and api 20e systems. bacillus cereus (54 st ... | 1988 | 3395100 |
| bacillus infections in patients with cancer. | eighteen febrile patients experienced 24 episodes of bacillus bacteremias from january 1978 to june 1986. bacillus species isolated included bacillus cereus (eight cases), bacillus circulans (three), bacillus subtilis (two), bacillus pumilus (two), bacillus licheniformis (one), bacillus sphaericus (one), bacillus coagulans (one), and six that could not be speciated. fifteen patients had lymphoma or leukemia and three had breast cancer. nine patients were neutropenic (polymorphonuclear neutrophil ... | 1988 | 3401098 |
| [isolation of bacillus cereus from fried rice from restaurants in costa rica]. | 1988 | 3140340 | |
| isolation, physico-chemical properties and biological activity of pamamycin-607, an aerial mycelium-inducing substance from streptomyces alboniger. | pamamycin-607, which showed aerial mycelium-inducing activity, has been isolated from streptomyces alboniger ifo 12738. at 0.1 microgram/disc it induces aerial mycelia in the aerial mycelium-negative strain of s. alboniger but inhibits growth of the substrate mycelia at 10 micrograms/disc. it also acts as an antibiotic against some fungi and bacteria. when kmno4 was partitioned with pamamycin-607 between benzene and water, mno4- but no k+ was transferred from the water to the benzene layer; pama ... | 1988 | 3141335 |
| [effect of the culture broth on the reactivation of refractile resting forms and on spore germination in bacillus cereus]. | the filtrate of the cultural broth taken at the lag phase of bacillus cereus 504 growth stimulated the germination of endospores and the reactivation of refractile resting forms. the energy processes were shown to be stimulated in the studied anabiotic cells. | 1988 | 3141749 |
| protection of cadmium toxicity to b. cereus, e. coli and a. niger by na+, mg++ and ca++ ions. | 10ppm of cadmium apparently seem to extend the lag phase of bacillus cereus and escherichia coli but in reality both 10 and 100ppm decreased the growth with differing intensity. 1.0ppm of cadmium decreased the growth of aspergillus niger, and it failed to grow at 500ppm. however, addition of calcium, magnesium and sodium gave increasing protection against cadmium toxicity for the growth of b. cereus, e. coli an a. niger. it is inferred that environment pollution by cadmium is related to the cati ... | 1988 | 3142161 |
| role of menaquinone in inactivation and activation of the bacillus cereus forespore respiratory system. | the respiratory systems of the bacillus cereus mother cell, forespore, and dormant and germinated spore were studied. the results indicated that the electron transfer capacity during sporulation, dormancy, and germination is related to the menaquinone levels in the membrane. during the maturation stages of sporulation (stages iii to vi), forespore nadh oxidase activity underwent inactivation concomitant with a sevenfold decrease in the content of menaquinone and without major changes in the cont ... | 1988 | 3142861 |
| microbial transformation of the pyrethroid insecticides: permethrin, deltamethrin, fastac, fenvalerate, and fluvalinate. | pure cultures of bacillus cereus, pseudomonas fluorescens, and achromobacter sp. were shown to transform five pyrethroid insecticides in the presence of tween 80. one of the major products, 3-phenoxybenzoic acid, was further transformed to 4-hydroxy-3-phenoxybenzoic acid. permethrin was the most rapidly transformed of the pyrethroids, with a half-life of less than 5 days. | 1988 | 3145715 |
| [the contamination of a hospital with bacillus cereus without an indication of cases of infection]. | a hygienic investigation of a hospital revealed b. cereus in 60.5% (66 out of 109) of swab specimens taken from walls, apparatuses, airing devices and bedclothes. these findings were not associated with evidence of human infection caused by the organism within the hospital. in a similar investigation three weeks later, only 22.5% of the environmental specimens were positive for b. cereus. the hospital contamination with b. cereus has thus been virtually temporary and epidemiologically silent. co ... | 1988 | 3146178 |
| [glycine-dependent cryotransformation of bacillus cereus by plasmid dna]. | a new technique for transformation of naturally noncompetent strains of bac. cereus is proposed. penetration of the dna into recipient cells is based on two-step effect. at the first step of the process bacilli are affected by glycine in the early logarithmic phase of growth of the common periodic culture. at the second step the mixed dna and recipient cells are frozen-thawed. the process permits the transforming dna penetration via the outer membrane layer of the recipient cells having the affe ... | 1988 | 3148860 |
| inhibitory effect of 1-methyldodecyldimethylamine oxide and n,n-bis(dodecyldimethyl)-1,2-ethanediammonium dibromide on the spores of bacillus cereus. | 1-methyldodecyldimethylamine oxide (mddo) and n,n'-bis(dodecyldimethyl)-1,2-ethanediammonium dibromide (bded) exhibit a significant affinity for the surface of bacillus cereus spores and adsorb very rapidly to the cells; they have a pronounced inhibitory effect on spore outgrowth. in order to alter the affinity of the spore surface for these inhibitors, the spores were pretreated with sodium dodecyl sulfate (sds), and with an electronegative (tween 80) and electropositive (histone) compound. in ... | 1988 | 3149252 |
| in vitro activity of fleroxacin (ro23-6240), a new fluorinated 4-quinolone against isolates from cancer patients. | the in vitro activity of fleroxacin (ro23-6240; am 833), a new fluorinated 4-quinolone, was compared to that of ciprofloxacin, enoxacin and a-56620, against 747 isolates from cancer patients. fleroxacin inhibited more than 90% of enterobacteriacea isolates at a concentration of less than or equal to 0.25 micrograms/ml. it was also extremely active against aeromonas hydrophila and haemophilus influenzae isolates with mic90 values of 0.12 and 0.06 micrograms/ml, respectively. the mic90 for acineto ... | 1988 | 3149568 |
| [laboratory study on an outbreak of bacillus cereus food poisoning]. | 1988 | 3150347 | |
| [amphoteric ion intermediates in the pyrazine series. the action of phenacyl bromide on pyrazine]. | 1988 | 3150600 | |
| functional relationships between l- and d-alanine, inosine and nh4cl during germination of spores of bacillus cereus t. | the results of a physiological study of the interaction between nh4cl, inosine, and the stereoisomers of alanine during germination of spores of bacillus cereus t are presented. detailed kinetics for the germination of unheated spores in moderate concentrations of l-alanine (in the absence of auto-inhibition due to alanine racemase) are established, as is the specificity of the stimulatory effect of nh4cl in relation to other salts, amines, and germinants. the results suggest that nh4cl and inos ... | 1988 | 3151208 |
| effect of reduced water activity on vegetative growth of cells and on germination of endospores of sporolactobacillus. | growth of vegetative cells and germination of endospores of sporolactobacillus at reduced water activity (aw) values were studied with nacl and glycerol as humectants, and compared with bacillus cereus and clostridium sporogenes. with nacl the highest aw for complete inhibition of both growth of vegetative cells and germinated spores of sporolactobacillus was 0.955. with glycerol, growth of vegetative cells was completely inhibited at 0.905 as compared to 0.880 for vegetative growth resulting fr ... | 1988 | 3152794 |
| critical control points for foods prepared in households whose members had either alleged typhoid fever or diarrhea. | hazard analysis of food preparation practices were conducted in four households and eleven others were visited to survey both food preparation practices and environmental conditions. households selected had members who were suffering from either diarrhea of unknown etiology or alleged typhoid fever. hazard analyses and sanitary surveys included gathering data on time-temperature exposures of foods, collecting samples of food and drinking water, sampling sewage or drains, and obtaining stool spec ... | 1988 | 3152803 |
| the inhibition of the growth of bacillus cereus in liver sausage. | the growth of bacillus cereus is a problem in liver sausage especially when the sausages are stored at high temperatures. even concentrations of greater than 10(6)/g have been detected. in this study we found that when combining glucono-delta-lactone, sodium erythorbate and citric acid with sodium nitrite and salt the growth of b. cereus could be delayed or totally inhibited. | 1988 | 3152808 |
| [polyvinylpyrrolidone as a protective agent during the drying of bacillus cereus by the sublimation method]. | 1988 | 2452296 | |
| release of proteins from the surface of bovine central nervous system myelin by salts and phospholipases. | incubation of bovine cns myelin with phospholipase c from bacillus cereus under conditions that lead to extensive phospholipid degradation caused 10% of the myelin protein to be released from the membrane. the myelin basic protein (mbp) was a major component of the dissolved protein. comparable incubations with phospholipase c from clostridium perfringens, phosphatidylinositol-specific phospholipase c from staphylococcus aureus, or cabbage phospholipase d removed little mbp. however, concentrati ... | 1988 | 2448423 |
| different phosphorylated forms of an insulin-sensitive glycosylphosphatidylinositol from rat hepatocytes. | labeling with [3h]galactose was employed to isolate a glycosylphosphatidylinositol from rat hepatocytes which might be involved in the action of insulin. the polar head group of this glycosylphosphatidylinositol was generated by phosphodiesterase hydrolysis with a phosphatidylinositol-specific phospholipase c from bacillus cereus. by dowex ag1 x 8 chromatography the polar head group could be separated into three radioactive peaks eluting at 100 mm (peak i), 200 mm (peak ii) and 500 mm (peak iii) ... | 1988 | 3042467 |
| an aspartic proteinase of erythrocyte membranes. proposed mechanism for activation and further molecular properties. | an aspartic proteinase associated with human erythrocyte membranes was shown to be responsible for autodegradation of the membrane proteins at ph values below 5.0. when the membrane was treated with phospholipase c (bacillus cereus) or trypsin, and simply heated at 40 degrees c, the membrane-bound latent enzyme was activated, with this being accompanied by dissociation of the enzyme from the membrane. divalent cations such as ca2+ and mg2+ had an inhibitory effect on the dissociation of the memb ... | 1988 | 3060144 |
| acetylcholinesterases from musca domestica and drosophila melanogaster brain are linked to membranes by a glycophospholipid anchor sensitive to an endogenous phospholipase. | the sensitivity of acetylcholinesterases (aches) from musca domestica and from drosophila melanogaster to the phosphatidylinositol-specific phospholipase c from bacillus cereus and to the glycosylphosphatidylinositol-specific phospholipase c from trypanosoma brucei was investigated. b. cereus phospholipase c solubilizes membrane-bound ache, and both phospholipases convert amphiphilic aches into hydrophilic forms of the enzyme. the lipases uncover an immunological determinant that is found on oth ... | 1988 | 2831298 |
| endovesiculation of human erythrocytes exposed to sphingomyelinase c: a possible explanation for the enzyme-resistant pool of sphingomyelin. | when human erythrocytes are treated with staphylococcus aureus sphingomyelinase c at 37 degrees c they become susceptible to cold lysis and appear to endovesiculate. endovesiculation has been confirmed by showing that in parallel with sphingomyelin breakdown, the cells accumulate [3h]inulin or [14c]sucrose (without losing intracellular k+) and also experience a loss of cell-surface acetylcholinesterase activity into a latent intracellular pool which can be revealed by treatment with detergent. o ... | 1988 | 2831979 |
| inactivation of blasticidin s by bacillus cereus. ii. isolation and characterization of a plasmid, pbsr8, from bacillus cereus. | 1988 | 2833485 | |
| bcefi, a new type iis restriction endonuclease. | a new type iis restriction endonuclease was identified, partially purified and characterized from a bacillus cereus subsp. fluorescens strain. the enzyme recognizes the nonpalindromic sequence acggc and cleaves at a distance from it. the cleavage appears to occur with a +/- 1 basepair uncertainty. thus the cleavage and recognition site is as shown below: acggc(n)11-13 tgccg(n)12-14. | 1988 | 2835751 |
| near stoichiometric, irreversible inactivation of bacterial collagenases by o-chloranil (3,4,5,6-tetrachloro-1,2-benzoquinone). | the hydrogen-abstracting quinone derivative 3,4,5,6-tetrachloro-1,2-benzoquinone (o-chloranil) caused a strong, near stoichiometric, irreversible inactivation of the collagenases from bacillus cereus, clostridium histolyticum and achromobacter iophagus. p-chloranil was a weaker inactivator. o-chloranil reacted rapidly with a site that affected substrate binding. amino acid analyses of native and totally inactivated enzymes, and the ph-profile of inactivation suggest that the dissociated form of ... | 1988 | 2837215 |
| nucleotide sequence and expression in escherichia coli of the gene coding for sphingomyelinase of bacillus cereus. | bacillus cereus secretes phospholipases c, which hydrolyze phosphatidylcholine, sphingomyelin and phosphatidylinositol. a 7.5-kb hindiii fragment of b. cereus dna cloned into escherichia coli, with puc18 as a vector, directed the synthesis of the sphingomyelin-hydrolyzing phospholipase c, sphingomyelinase. nucleotide sequence analysis of the subfragment revealed that it contained two open reading frames in tandem. the upstream truncated open reading frame corresponds to the carboxy-terminal port ... | 1988 | 2841128 |
| the solubilization of tetrameric alkaline phosphatase from human liver and its conversion into various forms by phosphatidylinositol phospholipase c or proteolysis. | when membrane-bound human liver alkaline phosphatase was treated with a phosphatidylinositol (pi) phospholipase c obtained from bacillus cereus, or with the proteases ficin and bromelain, the enzyme released was dimeric. butanol extraction of the plasma membranes at ph 7.6 yielded a water-soluble, aggregated form that pi phospholipase c could also convert to dimers. when the membrane-bound enzyme was solubilized with a non-ionic detergent (nonidet p-40), it had the mr of a tetramer; this, too, w ... | 1988 | 2844768 |
| bacillus cereus strain se-1: nucleotide sequence of the sphingomyelinase c gene. | 1988 | 2848222 | |
| egg yolk paste for determining some food poisoning bacteria. | egg yolk, aseptically prepared from fresh eggs, was partially dehydrated with a 40% high fructose corn syrup solution, and 10% salt was added. this salted yolk paste was added to mannitol salt agar for the detection of staphylococcus aureus, to nacl-glycine kim and goepfert medium for detection of bacillus cereus, to clostridium welchii agar for detection of c. perfringens, and to gifu anaerobic medium for detection of c. botulinum. these food poisoning bacteria showed the same lecithovitellin ( ... | 1988 | 2898139 |
| [microbial contamination of traditional herbal drugs in indonesia]. | 1988 | 2907976 | |
| cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase c of bacillus cereus. | a synthetic oligodeoxynucleotide probe was used to clone the gene encoding the phosphatidylcholine-preferring phospholipase c of bacillus cereus. the sequence of a 2050-bp restriction fragment containing the gene was determined. analysis of the gene-derived amino acid (aa) sequence showed that this exoenzyme is probably synthesized as a 283-aa precursor with a 24-aa signal peptide and a 14-aa propeptide. the mature, secreted enzyme comprises 245 aa residues. sonicates of escherichia coli hb101 c ... | 1988 | 3137122 |
| synthesis of some 5-azo(4'-substituted benzene-sulphamoyl)-8-hydroxyquinolines with antidotal and antibacterial activities. | 5-azo(4'-substituted benzenesulphamoyl)-8-hydroxyquinolines(iii) have been prepared by coupling of the appropriate p-substituted benzenesulphamoyldiazonium acetates with 8-hydroxyquinoline. the corresponding copper chelates(iv) and iron chelates(v) were also prepared in a 1:2 metal to ligand ratio. structures of iii, iv and v were confirmed by some representative uv, ir, and nmr spectrometry in addition to microanalysis. antidotal activity of four ligands (iiia, iiid, iiif, and iiii) has been ev ... | 1988 | 3137314 |
| plasmid, serotypic, and enterotoxin analysis of bacillus cereus in an outbreak setting. | bacillus cereus is a recognized agent of food-borne disease. in this report we describe an outbreak of b. cereus gastroenteritis associated with consumption of beef stew among patients and staff at a rhode island nursing home. the beef had been improperly stored after preparation. the predominant symptoms of the illness were cramps and diarrhea; it lasted an average of 16 h. no deaths occurred. the organism was recovered from 10 of 23 stools collected from ill patients and 1 of 21 stools collect ... | 1988 | 3139708 |
| bacillus species. | 1988 | 3139745 | |
| attachment of human placental-type alkaline phosphatase via phosphatidylinositol to syncytiotrophoblast and tumour cell plasma membranes. | phosphatidylinositol anchors human placental-type alkaline phosphatase (plap) to both syncytiotrophoblast and tumour cell plasma membranes. plap activity was released from isolated human placental syncytiotrophoblast plasma membranes and the surface of tumour cells with a phospholipase c from bacillus cereus. this was a specific event, not the result of proteolysis or membrane perturbation, but the action of a phosphatidylinositol-specific phospholipase c in the preparation. soluble plap, releas ... | 1988 | 3127211 |
| crystal structure of neutral protease from bacillus cereus refined at 3.0 a resolution and comparison with the homologous but more thermostable enzyme thermolysin. | neutral protease from bacillus cereus exhibits a 73% amino acid sequence homology to thermolysin, for which an accurate crystal structure exists. the b. cereus enzyme is, however, markedly less thermostable. the neutral protease was crystallized and diffraction data to 3.0 a resolution were recorded by oscillation photography. the crystal structure was solved by molecular replacement methods using thermolysin as a trial molecule. the solution was improved by rigid-body refinement and model rebui ... | 1988 | 3127592 |
| bacillus cereus bacteremia in an adult with acute leukemia. | bacillus cereus, which used to be considered non-pathogenic, was isolated from the blood of a patient with acute leukemia who was receiving intensive chemotherapy. fatal bacteremia developed with a clinical syndrome of acute gastroenteritis, followed by both meningoencephalitis with subarachnoid hemorrhage and multiple liver abscesses probably caused by infective vasculitis. surveillance stool cultures revealed colonization with the organism prior to the onset of diarrhea, and repetitive blood c ... | 1988 | 3127617 |
| transfer of spores, bacteria and yeast into toluene containing phospholipids and low amounts of water: preservation of the bacterial respiratory chain. | a method that allows the transfer of spores, bacteria and yeasts into a ternary system composed of toluene, phospholipids and low amounts of water is described. initially an emulsion is formed by sonication of cells suspended in water in presence of toluene and phospholipids. the emulsion formed was subsequently clarified by blowing n2 on its surface and transparency was achieved when the water content of the system was reduced to 1-3 microliter/ml of organic solvent. the cells in ternary system ... | 1988 | 3128288 |
| acquired b antigen associated with infection by bacillus cereus: in vivo and in vitro transformation of a1 red cells. | 1988 | 3130909 | |
| cloning, nucleotide sequence, and expression of the bacillus cereus 5/b/6 beta-lactamase ii structural gene. | two forms of heat-stable, zinc-containing beta-lactamase ii have been described for strains of bacillus cereus and have been shown to differ in substrate specificity (r. b. davies, e. p. abraham, j. fleming, and m. r. pollock, biochem. j. 145: 409-411, 1975). we report here the nucleotide sequence, inferred amino acid sequence, and expression of beta-lactamase ii from b. cereus 5/b/6 and compare our results with those for its homolog characterized in b. cereus 569/h (m. hussain, c. anthony, m. j ... | 1988 | 3131315 |
| incidence and characterization of bacillus cereus isolates contaminating dairy products. | a total of 293 dairy products purchased from local markets were examined to determine the incidence of and characterize bacillus cereus. isolations were made on mannitol-egg yolk-polymyxin b agar medium and confirmed by several staining and biochemical tests. b. cereus occurred in 17% of fermented milks, 52% of ice creams, 35% of soft ice creams, 2% of pasteurized milks and pasteurized fruit- or nut-flavored reconstituted milks, and 29% of milk powders, mostly in fruit- or nut-flavored milk mixe ... | 1988 | 3132097 |
| high-resolution solid-state 13c nuclear magnetic resonance of bacterial spores: identification of the alpha-carbon signal of dipicolinic acid. | natural-abundance solid-state 13c nuclear magnetic resonance spectra were obtained for bacterial spores for the first time by using the technique of cross-polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. a resonance at about 150 ppm, detectable in spore samples having a mn content of less than 0.05%, was consistent with an identification as the alpha-carbon signal of calcium dipicolinate; this signal was missing from a spore sample treated with acid to release dipicolin ... | 1988 | 3132103 |
| investigation of the bacillus cereus phosphonoacetaldehyde hydrolase. evidence for a schiff base mechanism and sequence analysis of an active-site peptide containing the catalytic lysine residue. | reaction of bacillus cereus phosphonoacetaldehyde hydrolase (phosphonatase) with phosphonoacetaldehyde or acetaldehyde in the presence of nabh4 resulted in complete loss of enzymatic activity. treatment of phosphonatase with nabh4 in the absence of substrate or product had no effect on catalysis. inactivation of phosphonatase with [3h]nabh4 and phosphonoacetaldehyde, nabh4 and [14c]acetaldehyde, or nabh4 and [2-3h]phosphonoacetaldehyde produced in each instance radiolabeled enzyme. the nature of ... | 1988 | 3132206 |
| false positive legionella pneumophila direct immunofluorescent monoclonal antibody test caused by bacillus cereus spores. | direct immunofluorescent monoclonal antibody stain testing for legionella pneumophila in oklahoma lake water yielded an unknown bacillus with fluorescence intensity equal to that of l. pneumophila stock strains. the organism in question was identified as bacillus cereus, a ubiquitous bacterium. when b. cereus cultures were studied, fluorescence was seen in spores but not in vegetative cells. since a positive immunofluorescent monoclonal antibody test (alone) might be considered by some individua ... | 1988 | 3133155 |
| microbial spoilage of pre-cooked potato-topped pies. | the ecological succession of bacteria which developed in pre-cooked potato-topped pies stored at two different temperatures was examined. bacillus, streptococcus and staphylococcus-micrococcus spp. were the predominant organisms isolated from freshly prepared pies and those stored at 4 degrees and 37 degrees c. none of these groups of bacteria caused significant biodeterioration of pies held at 4 degrees c, but all groups grew well in pies stored at 37 degrees c and achieved counts of ca 10(8)/g ... | 1988 | 3133345 |
| characterization of bacillus cereus strains isolated from drugs and evaluation of their toxins. | the microbial contamination of 68 samples of topical and 324 samples of oral medicaments has been studied. the most common group of contaminants was members of the genus bacillus (34.4%). because of the pathogenic significance of b. cereus, 39 strains were characterized by morphology and biochemical properties. all except three showed most of the characteristics of the type strain. they were highly resistant to lincomycin, polymyxin b and penicillin g-cephalosporin and were susceptible to strept ... | 1988 | 3133346 |
| the influence of pyrithione on the growth of micro-organisms. | pyrithione was active against a range of micro-organisms, the most resistant being gram-negative bacteria. the growth curves for klebsiella pneumoniae and bacillus licheniformis showed a drug-dependent lag phase. candida albicans grew with a drug-dependent growth rate. edta antagonized the effects of pyrithione. | 1988 | 3133347 |
| results of a prospective statewide reporting system for infective endocarditis. | to better understand the microbiology, anatomy, and demography of infectious endocarditis, we devised a prospective statewide reporting system to study these infections. because our study design required accurate diagnosis, reliable case reporting, and a high probability of physician-to-case exposure, we enlisted the help of cardiologists, cardiovascular surgeons, and infectious disease specialists throughout louisiana. all louisiana members of the american college of cardiology and the infectio ... | 1988 | 3133947 |
| beta-lactamase inhibitors. the inhibition of serine beta-lactamases by specific boronic acids. | many beta-lactamases have active-site serine residues, and are competitively inhibited by boronic acids. hitherto, the boronic acids used have lacked any structural resemblance to the substrates of beta-lactamases. phenylacetamidomethaneboronic acid, trifluoroacetamidomethaneboronic acid and 2,6-dimethoxybenzamidomethaneboronic acid have now been synthesized. the first of these contains the side-chain moiety of penicillin g, and the last that of methicillin. the ph-dependence of binding of the f ... | 1988 | 3135799 |
| [mechanisms of induction of germination of bacillus cereus spores using various initiators]. | 1988 | 3135990 | |
| 1-palmitoyl-2-thiopalmitoyl phosphatidylcholine, a highly specific chromogenic substrate of phospholipase a2. | 1-palmitoyl-2-thiopalmitoyl phosphatidylcholine (2-thiopc), a structurally modified phospholipid analog is specifically hydrolyzed by phospholipase a2 to liberate 2-thiolysophosphatidylcholine and palmitic acid. the sulfhydryl group of the product is readily trapped by 5,5'-dithiobis (2-nitrobenzoic acid) allowing continuous spectrophotometric monitoring of the enzymatic reaction. the rates of hydrolysis by bee-venom phospholipase a2 have been determined in a series of triton x-100 containing mi ... | 1988 | 3124835 |
| bacillus keratitis associated with contaminated contact lens care systems. | we examined two soft contact lens wearers who developed keratitis associated with bacillus contamination of their contact lens care systems. patient 1 developed a corneal ulcer caused by b. subtilis, and patient 2 demonstrated multiple, diffuse, punctate corneal epithelial opacities associated with b. cereus contamination in the contact lens and lens case compartment. the contact lens cases of both patients contained bacillus spores that survived multiple heat disinfection treatments. three diff ... | 1988 | 3124625 |
| excretion of tetracycline and chlortetracycline in eggs after oral medication of laying hens. | after dosing laying hens orally with tetracycline (tc) through either drinking water (0.25 and 0.5 g/l for 5 days) or feed (300 and 600 ppm for 7 days), and chlortetracycline (ctc) through feed (600 ppm) residues were determined by an agar plate diffusion technique in cylinders with bacillus cereus as test-organism, separately for albumen and for yolk. the sensitivity threshold was 0.07 micrograms/g in albumen and 0.15 micrograms/g in yolk for tc and 0.01 micrograms/g in albumen and 0.06 microgr ... | 1989 | 2912795 |
| structure and glycosylation of lipoteichoic acids in bacillus strains. | the occurrence, structure, and glycosylation of lipoteichoic acids were studied in 15 bacillus strains, including bacillus cereus (4 strains), bacillus subtilis (5 strains), bacillus licheniformis (1 strain), bacillus polymyxa (2 strains), and bacillus circulans (3 strains). whereas in the cells of b. polymyxa and b. circulans neither lipoteichoic acid nor related amphipathic polymer could be detected, the cells of other bacillus strains were shown to contain lipoteichoic acids built up of poly( ... | 1989 | 2914853 |
| specificity of bacillus thuringiensis for lepidopteran larvae: factors involved in vivo and in the structure of a purified protoxin. | the relative ld50 values in two test lepidoptera of bacillus thuringiensis subspecies kurstaki hd1, which contains three cryla protoxin genes, was the same as a plasmid-cured derivative or a bacillus cereus transcipient containing only one of the three genes. differential rates of transcription of these genes in the original strain could account, at least partly, for this result. strains containing only the single protoxin gene (cryla(b] produced inclusions when grown at 25 degrees c but not 32 ... | 1989 | 2615654 |
| isolation and fundamental properties of a phospholipase a2 inhibitor from the blood plasma of trimeresurus flavoviridis. | phospholipase a2 inhibitor was purified from the blood plasma of habu, trimeresurus flavoviridis, by sephadex g-200 gel filtration, deae-cellulose chromatography, and blue-sepharose cl-6b column chromatography. the purified inhibitor was shown to be a glycoprotein with a molecular weight of about 100k. it was found to consist of four subunits whose molecular weights were around 20-24k. in order to examine the inhibition mechanism of the inhibitor, the interaction of the inhibitor with a phosphol ... | 1989 | 2628432 |
| the nucleotide sequence of the lipo-penicillinase gene of alkalophilic bacillus sp. strain 170. | the lipo-penicillinase (lipen) gene from an alkalophilic bacillus sp. strain 170 was cloned in escherichia coli using the vector phsg399. a plasmid, pfap121, was isolated from an ampicillin resistant transformant and the cloned lipen gene was found to be in a 2.2 kb dna fragment. the nucleotide sequence of a 1.9 kb segment encoding the lipen was determined. this segment showed an open reading frame which would encode a polypeptide of 310 amino acids. the amino acid sequence of this lipen gene pr ... | 1989 | 2655551 |
| screening of isoquinoline alkaloids and their derivatives for antibacterial and antifungal activities. | a screening test of antimicrobial activities for some of the isoquinoline alkaloids and their first and second hofmann elimination products was conducted in this study. results showed that (+)-actinodaphnine (1), (+)-n-me-actinodaphnine (2), (+)-anonaine (17), (-)-xylopine (19) and (-)-n-me-xylopine mei (20), had the strongest inhibitory activities against three g(+) bacteria (bacillus cereus, micrococcus sp. and staphylococcus aureus) mic greater than or equal to 50 micrograms/ml). whereas anhy ... | 1989 | 2733073 |
| mortality in adult tsetse, glossina morsitans morsitans, caused by entomopathogenic bacteria. | mortality in adult tsetse, glossina morsitans morsitans, caused by pseudomonas aeruginosa, serratia marcescens, bacillus sphaericus, bacillus cereus, bacillus thuringiensis h-14, b. thuringiensis 1, b. thuringiensis 5, b. thuringiensis var. insraelensis, and providentia rettgeri was determined. when bacteria were smeared on rabbit skin and tsetse allowed to feed only once on the contaminated area, mortality 8 days postingestion was significantly higher (p less than 0.01) in tsetse fed on p. aeru ... | 1989 | 2738417 |
| effect of exposure of pieris brassicae larvae to 2,4,5-trichlorophenoxyacetic acid on the natural antibacterial activity of serum. | larvae of the cabbage white butterfly, pieris brassicae, were reared on a semisynthetic diet with or without 20 ppm of the herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) and using three assays the sera were subsequently tested for natural antibacterial activity against bacillus cereus, escherichia coli k12, and micrococcus luteus. these assays showed that exposure of larvae to 2,4,5-t lowered the antibacterial activity of the serum against e. coli and m. luteus compared with control anima ... | 1989 | 2738419 |
| demonstration of functional acetylcholinesterase on the soma of individual neurones of aplysia by in vivo microspectrophotometry. | the presence of functional acetylcholinesterase is demonstrated in vivo on somatic membranes of single ganglionic neurones of aplysia using concurrently microspectrophotometry and electrophysiology. the similarity of the effects of an irreversible blocker of acetylcholinesterase and of phospholipase c from bacillus cereus suggests that acetylcholinesterase is anchored in the membrane via phosphatidylinositol. | 1989 | 2765894 |
| isolation of a staphylococcus aureus beta-lactamase-dicloxacillin complex and kinetic studies on the reactivation of the enzyme. | exposure of the beta-lactamase from staphylococcus aureus to the slowly reacting substrates cloxacillin or dicloxacillin results in time-dependent inactivation of the enzyme. methods for the rapid separation of a beta-lactamase-dicloxacillin complex from excess inhibitor, using centrifuged columns of sephadex g-25 or deae-sephadex g-25, are described. the enzyme-dicloxacillin complex releases active enzyme, with specific activity identical to that of untreated enzyme, after storage at ph 7.5 at ... | 1989 | 2783544 |
| [l-forms of aerobic spore-forming bacteria isolated from urologic patients]. | new data are presented on the ability of different aerobic spore-forming bacteria isolated from the organism of urological patients to produce l-forms of these microorganisms in the presence of penicillin and ampicillin. bacillus cereus is shown to be the most resistant to these antibiotics. | 1989 | 2796790 |
| [structure and biological features of the temperate phage of bacillus thuringiensis var. galleriae 69/9, sensitive to chloroform]. | a new temperate bacteriophage designated px1 has been isolated from the culture of bacillus thuringiensis var. galleriae 69/6 producing enthobacterin. the bacteriophage belongs to morphological group b1 in accordance with the classification by d. reanney and h. ackerman. the bacteriophage head has an isometric multifaceted form with 40 nm diameter. the length of its noncontractile transversely lined tail is 130 nm. high sensitivity to chloroform is peculiar of the phage. the lytical specter of t ... | 1989 | 2811909 |
| bacereutin, an antifungal antibiotic isolated from metabolites of bacillus cereus chu 130. | an antifungal metabolite, bacereutin, was isolated from culture filtrate of bacillus cereus chu 130. the bacterium was isolated from soils collected in changhwa county, taiwan, and was grown in soybean meal-mannitol broth for production of the antibiotic metabolite. the antibiotic metabolite was isolated by adsorption column chromatography of amberite xad-2 and was purified by passing through the chromatographic columns packed with dowex 50w-x8, sephadex lh 20 and biogel p-2. the antibiotic meta ... | 1989 | 2484044 |
| bacillus cereus panophthalmitis associated with intraocular gas bubble. | it has become increasingly apparent that bacillus cereus can cause a severe and devastating form of endophthalmitis following penetrating trauma by a metallic object. b. cereus is an uncommon aetiological agent in non-clostridial gas-forming infections. the patient studied in this single case report showed evidence of intraocular gas mimicking gas gangrene infection. the physiology of non-clostridial bacteria producing gas from anaerobic metabolic conditions is reviewed. further intraocular and ... | 1989 | 2493262 |
| high-resolution (1.5 a) crystal structure of phospholipase c from bacillus cereus. | both the phosphatidylinositol-hydrolysing and the phosphatidylcholine-hydrolysing phospholipases c have been implicated in the generation of second messengers in mammalian cells. the phosphatidylcholine-hydrolysing phospholipase c (plc) from bacillus cereus, a monomeric protein containing 245 amino-acid residues, is similar to some of the corresponding mammalian proteins. this, together with the fact that the bacterial enzyme can mimic the action of mammalian plc in causing, for example, enhance ... | 1989 | 2493587 |
| the migration of bacteria through gels in the presence of iucd monofilament tails. | an in vitro model was developed to investigate the migration of a variety of bacteria of different characteristics through a gel system in the presence or absence of a wide range of polymer monofilament threads. the bacteria were unable to migrate through the gel from the point of inoculation in the absence of a solid substrate. migration occurred along all thread types tested, including those used as iucd marker tails and the extent of bacterial migration appeared to be determined primarily by ... | 1989 | 2495894 |
| phosphatidylinositol-specific phospholipase c from bacillus cereus: improved purification, amino acid composition, and amino-terminal sequence. | phosphatidylinositol-specific phospholipase c was purified in a 27% yield from the culture medium of bacillus cereus by a combination of ammonium sulfate precipitation and ion-exchange and hydrophobic interaction chromatography. the purified enzyme was free of other phospholipase c-type activities and exhibited a high specific activity of approximately 1,300 units/mg. amino acid composition analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated a molecular weight of abo ... | 1989 | 2496136 |
| mechanism of inactivation of alanine racemase by beta, beta, beta-trifluoroalanine. | the alanine racemases are a group of plp-dependent bacterial enzymes that catalyze the racemization of alanine, providing d-alanine for cell wall synthesis. inactivation of the alanine racemases from the gram-negative organism salmonella typhimurium and gram-positive organism bacillus stearothermophilus with beta, beta, beta-trifluoroalanine has been studied. the inactivation occurs with the same rate constant as that for formation of a broad 460-490-nm chromophore. loss of two fluoride ions per ... | 1989 | 2496744 |
| conformational states of beta-lactamase: molten-globule states at acidic and alkaline ph with high salt. | we present evidence that beta-lactamase is close to fully unfolded (i.e., random coil conformation) at low ionic strength at the extremes of ph and that the presence of salt causes a cooperative transition to a conformation with the properties of a molten globule, namely, a compact state with native-like secondary structure but disordered side chains (tertiary structure). the conformation of beta-lactamase i from bacillus cereus was examined over the ph 1.5-12.5 region by circular dichroism (cd) ... | 1989 | 2496758 |
| [isolation, purification and identification of protective substances from a culture broth of germinating bacillus cereus spores]. | a fraction increasing the resistance of resting spores to uv-irradiation and high temperature has been isolated from the culture medium at the stage of b. cereus st. 96 spore initiation. amino acid analysis, gas chromatography, electrophoresis, and tlc of the products of acidic and alkaline hydrolysis of the isolated fraction demonstrated that the active component of the fraction was the lipoteichoic acid. | 1989 | 2497164 |
| survival of beta-lactamase-producing and -nonproducing bacteria in intravenous solutions. | the survival of beta-lactamase-producing (beta-lac+) and non-beta-lactamase-producing (beta-lac-) bacillus and staphylococcus spp. has been investigated in dextrose 5% injection, nacl 0.9%, and dextrose 5% in nacl 0.9% solutions. tests were performed under static and turbulent conditions of incubation, with and without antibiotics added to the fluids, and with or without 1% citrated blood. all solutions were inoculated with about 1000 organisms/ml, and sampled for viable bacteria at specific tim ... | 1989 | 2497589 |
| [change in the ultrastructure of the cell wall of bacillus cereus, determined by rp4 mucts62 plasmid]. | the influence of plasmid rp4 mucts62, heterologous for b. cereus, on the growth rate of b. cereus strains ga 682 and 319 obtained in our earlier experiments and on changes in the ultrastructure of their cell walls in comparison with b. cereus initial strains gp 7 and dsm 318 has been studied. plasmid rp4 mucts62 with a wide spectrum of action has been found not only to determine the functional signs of resistance to antibiotics and thermal sensitivity in the heterologous host, but also to take p ... | 1989 | 2499144 |
| a thiono-beta-lactam substrate for the beta-lactamase ii of bacillus cereus. evidence for direct interaction between the essential metal ion and substrate. | an 8-thionocephalosporin was shown to be a substrate of the beta-lactamase ii of bacillus cereus, a zinc metalloenzyme. although it is a poorer substrate, as judged by the kcat./km parameter, than the corresponding 8-oxocephalosporin, the discrimination against sulphur decreased when the bivalent metal ion in the enzyme active site was varied in the order mn2+ (the manganese enzyme catalysed the hydrolysis of the oxo compound but not that of the thiono compound), zn2+, co2+ and cd2+. this result ... | 1989 | 2499308 |
| bacillus cereus septicemia associated with rhabdomyolysis and myoglobinuric renal failure. | a patient with acute myeloblastic leukemia (aml) developed septicemia due to bacillus cereus with subsequent rhabdomyolysis and myoglobinuric renal failure. he died despite intensive care. postmortem examination revealed diffuse muscle necrosis with infiltration of gram-positive bacilli and widespread bacterial microthrombi in various organs. septicemia associated with rhabdomyolysis has been described in 12 cases. this case represents the first reported case of b. cereus septicemia associated r ... | 1989 | 2499725 |
| clinical significance of bacillus species isolated from blood cultures. | to determine the clinical significance of blood isolates of bacillus, we reviewed all blood cultures obtained at north carolina memorial hospital between 1981 and 1985. over the five-year study period the number of patients (incidence per 10,000 hospital admissions) from whom bacillus was isolated increased from 4.97 in 1981 to 12.5 in 1985. the incidence per 1,000 blood cultures also increased from 1.12 in 1981 to 2.33 in 1985. review of the medical records of 78 of the 95 patients (82%) with p ... | 1989 | 2499933 |
| development of a fluorescent immunodot assay for bacillus cereus enterotoxin. | a fluorescent immunodot assay has been developed for rapid, specific detection of b. cereus enterotoxin. none of the other bacillus species tested showed cross-reactivity in the assay with antiserum to purified b. cereus enterotoxin. the assay can detect greater than or equal to 50 ng of purified enterotoxin. using this assay system, enterotoxin was found to be produced by 25 of 25 foodborne disease-related isolates and 22 of 25 isolates not related to foodborne disease (isolates from routine su ... | 1989 | 2500487 |
| [septic pneumonia caused by bacillus cereus]. | the authors describe the case of a 3-year old girl whose septic pneumonia was caused by b. cereus and developed following serial viral and bacterial infections, which produced assumably transient immunoppressed condition. attention is drawn to the possibility of serious diseases caused by faculative bacteria in certain cases. the increasing pathological significance of bacteria existing in the soil is pointed out and the importance of hemoculture examination is stressed. | 1989 | 2500633 |
| mutations affecting the catalytic activity of bacillus cereus 5/b/6 beta-lactamase ii. | random in vitro mutagenesis of a cloned bacillus cereus 5/b/6 beta-lactamase ii gene was used to select defective genes unable to confer ampicillin or cephalosporin c resistance to escherichia coli. dna sequencing of mutant genes identified histidine at position 28 as important to beta-lactamase ii function. in addition, the isolation of six identical frameshift mutants established that the carboxyl-terminal end of beta-lactamase ii is critical for enzyme function. random mutagenesis also reveal ... | 1989 | 2501295 |
| microbiological quality and public health significance of ice-cream. | fifty samples of ice-cream were subjected to microbiological examination. of these 72% had total viable count over 10(6)/g while 66% had coliform count between 10(2)-10(3)/g. the micro-organisms isolated were escherichia coli (46%), enterobacter aerogenes (34%), staphylococcus aureus (26%), proteus species (16%), streptococcus faecalis (12%), citrobacter species (10%) and bacillus cereus (4%). the results showed that commercially prepared ice-cream sold in the market was not satisfactory for hum ... | 1989 | 2501524 |