Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the phage t4 restriction endoribonuclease regb: a cyclizing enzyme that requires two histidines to be fully active. | the regb gene, from the bacteriophage t4, codes for an endoribonuclease that controls the expression of a number of phage early genes. the regb protein cleaves its mrna substrates with an almost absolute specificity in the middle of the tertranucleotide ggag, making it a unique well-defined restriction endoribonuclease. this striking protein has no homology to any known rnase and its catalytic mechanism has never been investigated. here, we show, using 31p nuclear magnetic resonance (nmr), that ... | 2003 | 12771201 |
| hazard rates of disease progression after external beam radiotherapy for clinically localized carcinoma of the prostate. | we established hazard rates for disease progression at different intervals after external beam radiotherapy alone in patients with clinically localized prostate cancer. we determined the likelihood of biochemical failure in those free of disease 5 years after radiotherapy and identified those at risk for early versus late biochemical failure. | 2003 | 12771739 |
| role of clinically determined depth of tumor invasion in the treatment of esophageal carcinoma. | we sought to evaluate the effectiveness of clinical staging of depth of tumor invasion (ct), the relationship of ct to survival, the benefits of downstaging ct, and the role of ct in treatment decisions. | 2003 | 12771883 |
| effects of selenium compounds on induction of dna damage by broadband ultraviolet radiation in human keratinocytes. | background ultraviolet radiation (uvr), a ubiquitous environmental genotoxin for the skin, produces dna damage. the trace element selenium induces synthesis of the glutathione peroxidase and thioredoxin reductase enzyme families. these selenoenzymes detoxify a range of toxic compounds generated by free radicals. objectives to assess the effects of pretreatment of primary human keratinocytes with selenium on uvr-induced dna damage. methods cells were irradiated with uvr from fs-20 lamps and were ... | 2003 | 12786833 |
| thyroxine downregulates sox9 and promotes chondrocyte hypertrophy. | thyroid hormones exert a profound effect on development, growth, and metabolism of skeleton. in the present study, we evaluated the effects of thyroxine (t4) and growth hormone (gh) on the terminal differentiation of rib growth plate chondrocytes in three-dimensional pellet culture. t4 (30ng/ml) stimulated the expressions of type ii and x collagens, alkaline phosphatase (alp) activity. on the other hand, the expression of chondrogenic transcription factor sox9 in the t4 treatment group decreased ... | 2003 | 12788086 |
| long-term changes in pulmonary function tests after definitive radiotherapy for lung cancer. | to evaluate the long-term changes in pulmonary function tests (pfts) in patients surviving at least 2 years after definitive radiotherapy (rt) for unresectable lung cancer. | 2003 | 12788165 |
| concurrent cisplatin, 5-fluorouracil, leucovorin, and radiotherapy for invasive bladder cancer. | to investigate the tolerance and efficacy of a modified concurrent chemoradiation (ccrt) protocol for patients with invasive bladder cancer "unfit" for radical cystectomy. | 2003 | 12788178 |
| accelerated superfractionated radiotherapy with concomitant boost for invasive bladder cancer. | to determine the toxicity and clinical effectiveness of accelerated superfractionated radiotherapy with delayed concomitant boost (ascbrt) in locally invasive carcinoma of the bladder. | 2003 | 12788179 |
| contribution of conformal therapy in the treatment of anal canal carcinoma with combined chemotherapy and radiotherapy: results of a phase ii study. | to evaluate the feasibility of delivering conformal radiotherapy (rt) and concurrent chemotherapy without a mandatory break in patients with anal canal carcinoma. | 2003 | 12788191 |
| hypothalamic-pituitary-thyroid system activity during lithium augmentation therapy in patients with unipolar major depression. | lithium augmentation is an established strategy in the treatment of refractory depression, but little is known about predictors of response and its mode of action. there is increasing evidence that low thyroid function indices within the normal range are associated with a poorer treatment response to antidepressants, but previous studies on the hypothalamic-pituitary-thyroid (hpt) system during lithium augmentation provide inconclusive results and have methodological limitations. this study aime ... | 2003 | 12790161 |
| new system for positive selection of recombinant plasmids and dual expression in yeast and bacteria based on the restriction ribonuclease regb. | by coupling the toxic restriction endoribonuclease regb, from the bacteriophage t4, to the prokaryotic t7 and the eukaryotic gal1 promoters, we constructed a two-function plasmid called ptoxr-1. this plasmid is a zero-background cloning vector. it allows an efficient positive selection of recombinant plasmids without the need to completely digest, dephosphorylate, or purify the vector prior to the ligation step. the ptoxr-1 positive selection system requires no special escherichia coli strains, ... | 2003 | 12790631 |
| several salmonella enterica subsp. enterica serotype 4,5,12:i:- phage types isolated from swine samples originate from serotype typhimurium dt u302. | pulsed-field gel electrophoresis, plasmid profiling, and phage typing were used to characterize and determine possible genetic relationships between 48 salmonella enterica subsp. enterica isolates of pig origin collected in catalonia, spain, from 1998 to 2000. the strains were grouped into 23 multidrug-resistant fljb-lacking s. enterica serovar 4,5,12:i:- isolates, 24 s. enterica serovar typhimurium isolates, and 1 s. enterica serovar 4,5,12:-:- isolate. after combining the xbai and blni macrore ... | 2003 | 12791855 |
| influence of identification and preservation of pelvic autonomic nerves in rectal cancer surgery on bladder dysfunction after total mesorectal excision. | given the improvement in oncologic outcome after the introduction of total mesorectal excision for the treatment of rectal cancer, the objective of the present study was to determine the frequency of identification and preservation of the pelvic autonomic nerves and to identify a possible link between postoperative micturition disturbances and the extent of the radical resection. | 2003 | 12792438 |
| neonatal hyperbilirubinemia and its association with thyroid hormone levels and urinary iodine excretion. | to investigate, if, urinary iodine contents as a marker of iodine deficiency and hypothyroidism are associated with the incidence of neonatal hyperbilirubinemia. | 2003 | 12793308 |
| is there an association between life events, postnatal depression and thyroid dysfunction in thyroid antibody positive women? | postnatal depression is more common in women positive for thyroid autoantibodies, independent of thyroid hormone dysfunction, but the basis of this association is unclear. | 2003 | 12793517 |
| thyroid hormone synthesis and storage in the thyroid gland of human neonates. | the aim of this study was to evaluate the morphological and biochemical maturation of the thyroid gland in human neonates. the mean iodine concentration in the thyroid gland of very premature infants (less than 32 weeks gestational age, 0-3 days survival, n = 12) was significantly lower than in the older group (34-41 weeks gestational age, 0-30 days survival; n = 15; p < 0.05). for the whole group of neonates there was a statistically significant linear correlation between duration of life, i.e. ... | 2003 | 12793603 |
| [two cases of intramedullary spinal cord metastasis of lung cancer detected with mri]. | we report two cases of intramedullary spinal cord metastasis of lung cancer detected by mri. case 1: a 77-year-old man underwent chemotherapy and left lower lung lobectomy for squamous cell carcinoma of the lung (t2n0m0). about one year later, he complained of paresthesia of the lower extremities and claudication on walking, and then of weakness of the lower limbs and bladder dysfunction. magnetic resonance imaging (mri) revealed an enhanced mass in the dural sac at the level of the spines of l1 ... | 2003 | 12795190 |
| the endopolygalacturonase 1 from botrytis cinerea activates grapevine defense reactions unrelated to its enzymatic activity. | a purified glycoprotein from botrytis cinerea (strain t4), identified as endopolygalacturonase 1 (t4bcpg1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (vitis vinifera cv. gamay). these reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. most of these defense reactions were also activated in grapevine in response to ... | 2003 | 12795381 |
| five-year maintenance follow-up of early-onset periodontitis patients. | the purpose of this study was to evaluate the clinical and microbiological status of patients with early-onset or aggressive periodontitis (eop) who had received supportive periodontal care (spc) every 3-6 months for a period of 5 years, following active periodontal treatment. | 2003 | 12795796 |
| clinical characteristics of pneumonic-type adenocarcinoma of the lung. | to analyze diagnostic approaches, survival predictors, and treatment efficacy in pneumonic-type adenocarcinoma (p-adc). | 2003 | 12796162 |
| neoadjuvant doxil chemotherapy prior to androgen ablation plus radiotherapy for high-risk localized prostate cancer: feasibility and toxicity. | patients with clinical t3 or t4 prostate cancer or with elevated serum prostate-specific antigen (psa) levels greater than 40 ng/ml are at high risk of failure with primary treatment. chemotherapy administered at the time of diagnosis may decrease the risk of recurrence. patients with high risk localized prostate cancer were treated with two cycles of liposomal doxorubicin (doxil) at 50 mg/m2 every 28 days. patients were assessed for response by digital rectal examination (dre), psa, and endorec ... | 2003 | 12796607 |
| evaluation of rat thyroid gland morphophysiological status after three months exposure to 50 hz electromagnetic field. | objective of our study was to use morphophysiological criteria in order to determine the sensitivity of male rat thyroid gland to an extremely low frequency electromagnetic field (elf-emf) influence and the ability of the gland to repair after period of exposure. animals were exposed to 50hz, 50-500 microt elf-emf for 3 months when a part of them (group i) were sacrificed, while the rest of animals were subjected to recovery evaluation of the gland and sacrificed after 1 (group ii), 2 (group iii ... | 2003 | 12798131 |
| the diversity and evolution of the t4-type bacteriophages. | recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect eubacteria and archaea, constitute a substantial fraction of this population. in spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is known about them. for the last 10 years our research has focused on an extremely widespread group of phages, the t4-type. it has now become evident that phage t4 has a myriad of relat ... | 2003 | 12798230 |
| canonical nucleosides can be utilized by t4 dna ligase as universal template bases at ligation junctions. | t4 dna ligase catalyzes the template-dependent ligation of dna. using t4 dna ligase under specific experimental conditions, we demonstrate that each of the four canonical nucleosides, centrally located on a template molecule such that they flank the site of ligation, can direct the ligation of nucleic acids regardless of the identity of the terminal nucleosides being covalently joined. this universal templating capability extends to those positions adjacent to the ligation junction. this is the ... | 2003 | 12799448 |
| long-term results of intrathoracic chemohyperthermia (itch) for the treatment of pleural malignancies. | there is no standard treatment for patients with pleural malignancies. the aim of this prospective study was to investigate the toxicity and long-term results of a multimodality treatment consisting of surgery and intrathoracic chemohyperthermia (itch) for the treatment of patients with pleural malignancies. from january 1990 to august 2000, 24 patients with mesothelioma (n=17), fibrosarcoma (n=3), pleural adenocarcinoma (n=3) and thymoma (n=1) were included. the mesothelioma stages were t1 or t ... | 2003 | 12799624 |
| nonaqueous synthesis and selective crystallization of gallium sulfide clusters into three-dimensional photoluminescent superlattices. | a low-temperature, nonaqueous synthesis approach is described that produces a series of gallium sulfide and polysulfide three-dimensional superlattices from binary ga-s and ternary zn-ga-s supertetrahedral clusters. the diversity of superlattices is achieved by modifying the cluster size, the cluster composition, and the inter-cluster linkage mode. both pure t3 (ga10s186-) (denoted as ucr-7gas) and pure t4 (zn4ga16s3310-) (denoted as ucr-5zngas) superlattices with ring sizes of 18 and 24 tetrahe ... | 2003 | 12553794 |
| seasonal change and fate of coliphages infected to escherichia coli o157:h7 in a wastewater treatment plant. | seasonal change of virulent phage infected to two e. coli o157:h7 strains (o:157-phage) in the influent of a domestic wastewater treatment plant in the central part of japan and fate of o:157-phage in the plant were monitored almost monthly from march 2001 to february 2002. coliphage infected to nonpathogenic e. coli o157:h7 atcc43888 (43888-phage) was detected for 1 year. on the other hand, phage infected to pathogenic e. coli o157:h7 edl933 (edl-phage) was detected intermittently. concentratio ... | 2003 | 12553989 |
| determination of serum thyroxine enantiomers in patients by liquid chromatography with a chiral mobile phase. | a chromatographic method for the separation and determination of d- and l-thyroxine enantiomers (d-, and l-t4) in human serum with a chiral ligand ion-exchange system using a chiral mobile phase additive and a silica column was established. an aqueous eluent containing l-proline (l-pro) sufficiently complexed copper ii ions and triethylamine (tea) was used. it was monitored with a uv detector. the separation was completed in 12 min. the method has acceptable sensitivity, precision and accuracy f ... | 2003 | 12554149 |
| defining the active site of schizosaccharomyces pombe c-terminal domain phosphatase fcp1. | fcp1 is an essential protein serine phosphatase that dephosphorylates the c-terminal domain (ctd) of rna polymerase ii. by testing the effects of serial n- and c-terminal deletions of the 723-amino acid schizosaccharomyces pombe fcp1, we defined a minimal phosphatase domain spanning amino acids 156-580. we employed site-directed mutagenesis (introducing 24 mutations at 14 conserved positions) to locate candidate catalytic residues. we found that alanine substitutions for arg(223), asp(258), lys( ... | 2003 | 12556522 |
| definitive chemoradiotherapy for patients with malignant stricture due to t3 or t4 squamous cell carcinoma of the oesophagus. | we retrospectively investigated the efficacy and feasibility of concurrent chemoradiotherapy for patients with severe dysphagia caused by oesophageal squamous cell carcinoma. concurrent chemoradiotherapy was performed in 57 patients with t3 or t4 disease containing m1 lymph node (lym) disease. chemotherapy consisted of protracted infusion of 5-fluorouracil (5-fu) 400 mg m(-2) 24 h(-1) on days 1-5 and 8-12, combined with 2-h infusion of cisplatin (cddp) 40 mg m(-2) on days 1 and 8. radiation trea ... | 2003 | 12556953 |
| examination of the role of the clamp-loader and atp hydrolysis in the formation of the bacteriophage t4 polymerase holoenzyme. | transient kinetic analyses further support the role of the clamp-loader in bacteriophage t4 as a catalyst which loads the clamp onto dna through the sequential hydrolysis of two molecules of atp before and after addition of dna. additional rapid-quench and pulse-chase experiments have documented this stoichiometry. the events of atp hydrolysis have been related to the opening/closing of the clamp protein through fluorescence resonance energy transfer (fret). in the absence of a hydrolysable form ... | 2003 | 12559912 |
| thyroid hormone supplementation in sepsis: an experimental study. | the levels of thyroid hormones decrease in septic, critically ill, and major trauma patients who show a bad prognosis. the aim of this study was to investigate the effects of thyroid hormone supplementation on the sepsis criteria and mortality in an experimental sepsis model. | 2003 | 12560903 |
| austrian moderate altitude study (amas 2000) - fluid shifts, erythropoiesis, and angiogenesis in patients with metabolic syndrome at moderate altitude (congruent with 1700 m). | it was hypothesized that subjects with metabolic syndrome (hypertension, obesity, hyperlipidemia, diabetes mellitus): (1) develop measurable peripheral edema at moderate altitude and (2) might show differences on erythropoiesis, iron status and vascular endothelial growth factor (vegf) in comparison to healthy subjects during and after a long-term stay (3-week exposure) at moderate altitude (congruent with 1700 m). twenty-two male subjects with metabolic syndrome were selected. baseline investig ... | 2003 | 12560947 |
| [clinical and pathological related factors of occult cervical lymph node metastasis in squamous cell carcinoma of tongue]. | some clinical and histopathological features of squamous cell carcinoma of tongue (tscc) were closely associated with occult cervical lymph node metastasis (oclnm). this study was designed to investigate the correlation between occult cervical lymph node metastasis in squamous cell carcinoma of tongue and the clinicopathological indexes of primary tumor tissue. | 2003 | 12561440 |
| [relationship between the outcome and the frequency of micronuclei in cells of patients with oral and oropharyngeal carcinoma]. | to verify the correlation between the micronucleus frequency and the presence of local recurrences or second primary lesion in patients with carcinoma of the oral cavity. | 2003 | 12563459 |
| [treatment of autonomous and cystic thyroid nodules with intranodular ethanol injection]. | intranodular ethanol injection has been used for the past 10 years as an efficient modality for treating patients with thyroid nodules. several studies have reported the success of this therapy in autonomous and cystic nodules and, more recently, in cold benign nodules. | 2003 | 12563462 |
| new algorithm to control a cycle ergometer using electrical stimulation. | data were collected from four male subjects to determine the relationships between load, speed and muscle use during cycle ergometry. these data were then used to construct equations to govern the stimulation of muscle in paralysed individuals, during cycle ergometry induced by functional electrical stimulation (fes) of the quadriceps, gluteus maximus and hamstring muscles. the algorithm was tested on four subjects who were paralysed owing to a complete spinal cord injury between t4 and t11. usi ... | 2003 | 12572743 |
| activation of the regb endoribonuclease by the s1 ribosomal protein is due to cooperation between the s1 four c-terminal modules in a substrate-dependant manner. | the regb protein, encoded by the t4 bacteriophage genome, is a ribonuclease involved in the inactivation of the phage early messenger rnas. its in vitro activity is very low but can be enhanced up to 100-fold in the presence of the ribosomal protein s1. the latter is made of six repeats of a conserved module found in many other proteins of rna metabolism. considering the difference between its size (556 amino acids) and that of several regb substrates (10 nucleotides), we wondered whether all si ... | 2003 | 12576472 |
| disruption of 3d tissue integrity facilitates adenovirus infection by deregulating the coxsackievirus and adenovirus receptor. | the human coxsackievirus and adenovirus receptor (car) represents the primary cellular site of adenovirus attachment during infection. an understanding of the mechanisms regulating its expression could contribute to improving efficacy and safety of adenovirus-based therapies. we characterized regulation of car expression in a 3d cell culture model of human breast cancer progression, which mimics aspects of the physiological tissue context in vitro. phenotypically normal breast epithelial cells ( ... | 2003 | 12576544 |
| lymphoma of the nasal cavity and paranasal sinuses: treatment and outcome of early-stage disease. | the records of 23 patients diagnosed and treated at the massachusetts general hospital for extranodal non-hodgkin's lymphoma of the paranasal sinus and nasal cavity were reviewed. the majority of patients were ann arbor stage i and approximately evenly divided in t1 or t2 (n = 10) and t3 or t4 (n = 13). eight patients had nasal-type nk/t cell and 15 patients had diffuse large b-cell lymphoma (dlbcl). the patients with nasal-type nk/t cell lymphoma predominately involved the nasal cavity (5/8), w ... | 2003 | 12576916 |
| nonthyroidal illness syndrome in off-pump coronary artery bypass grafting. | cardiopulmonary bypass (cpb) is an established cause of nonthyroidal illness syndrome (ntis). off-pump coronary artery bypass (opcab) has been reported to be less invasive than coronary artery bypass grafting (cabg) with cpb. we prospectively evaluated thyroid metabolism in opcab patients. | 2003 | 12537197 |
| relationship between the physical shape and the efficiency of oligomeric chitosan as a gene delivery system in vitro and in vivo. | chitosans of high molecular weights have emerged as efficient nonviral gene delivery systems, but the properties and efficiency of well-defined low molecular weight chitosans (<5 kda) have not been studied. we therefore characterized dna complexes of such low molecular weight chitosans and related their physical shape and stability to their efficiency as gene delivery systems in vitro and in vivo. | 2003 | 12539151 |
| collagen triple helix formation can be nucleated at either end. | the directional dependence of folding rates for rod-like macromolecules such as parallel alpha-helical coiled-coils, dna double-helices, and collagen triple helices is largely unexplored. this is mainly due to technical difficulties in measuring rates in different directions. folding of collagens is nucleated by trimeric non-collagenous domains. these are usually located at the cooh terminus, suggesting that triple helix folding proceeds from the cooh to the nh(2) terminus. evidence is presented ... | 2003 | 12540847 |
| evaluation of the effects of clomipramine on canine thyroid function tests. | to evaluate the effect of long-term clomipramine administration on the hypothalamic-pituitary-thyroid axis in healthy dogs, 14 healthy adult dogs were enrolled in a prospective study. clomipramine (3 mg/kg po q12h) was administered to all dogs beginning on day 0, and continued for 112 days. serum total thyroxine (t4), free thyroxine (ft4), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (reverse t3; rt3), and thyroid-stimulating hormone (tsh) were measured on days 0, 7, 28, 42, 56, and 11 ... | 2003 | 12564726 |
| total glossectomy without laryngectomy as first-line or salvage therapy. | advanced carcinoma of the tongue can require total glossectomy. although radiation therapy is of limited efficacy in t3 and t4 tumors involving the base of the tongue, many surgeons are reluctant to suggest highly mutilating surgery. | 2003 | 12567098 |
| elevated c-reactive protein and homocysteine values: cardiovascular risk factors in hypothyroidism? a cross-sectional and a double-blind, placebo-controlled trial. | hypothyroidism is associated with premature atherosclerosis and cardiovascular disease. recently, total homocysteine (thcy) and c-reactive protein (crp) emerged as additional cardiovascular risk factors. we first investigated crp and thcy in different severities of primary hypothyroidism and in a second study we evaluated the effect of l-thyroxine treatment in patients with subclinical hypothyroidism (sch) in a double-blind, placebo-controlled trial. one hundred and twenty-four hypothyroid patie ... | 2003 | 12535752 |
| methemoglobin formation in children with congenital heart disease treated with inhaled nitric oxide after cardiac surgery. | inhaled nitric oxide (no) is used as a therapy of pulmonary hypertension in children after cardiac surgery. hemoglobin binds to no with great affinity and forms methemoglobin by oxidation in the erythrocyte. once produced, methemoglobin is unable to transport and unload oxygen in the tissues. the amount of available hemoglobin in the body for oxygen transport is thereby reduced. anemia, acidosis, respiratory compromise and cardiac disease may render patients more susceptible than expected for a ... | 2003 | 12536266 |
| phenylthiourea disrupts thyroid function in developing zebrafish. | thyroid hormone (t4) can be detected in thyroid follicles in wild-type zebrafish larvae from 3 days of development, when the thyroid has differentiated. in contrast, embryos or larvae treated with goitrogens (substances such as methimazole, potassium percholorate, and 6-n-propyl-2-thiouracil) are devoid of thyroid hormone immunoreactivity. phenythiourea (pturea; also commonly known as ptu) is widely used in zebrafish research to suppress pigmentation in developing embryos/fry. pturea contains a ... | 2003 | 12536323 |
| cystic duct carcinoma: a proposal for a new "working definition". | farrar's criteria for cystic duct carcinoma (histopathological diagnosis of a carcinoma strictly limited to the cystic duct) have practical limitations. we propose new "working definition": a gallbladder tumor, the center of which is located in the cystic duct. | 2003 | 12536328 |
| recombinant human thyroid-stimulating hormone: pharmacology, clinical applications and potential uses. | the major functions of pituitary thyroid-stimulating hormone (tsh) are to maintain the biosynthesis and secretion of the thyroid hormones l-thyroxine (t4) and l-3,5,3'triidothyronine (t3). the tsh core contains two apoproteins, the alpha and beta subunits. the alpha subunit is identical to that of pituitary follitropin, pituitary lutropin and placental chorionic gonadotropin, whereas the beta subunit is unique. tsh is a glycoprotein; the glycoprotein components of the alpha and beta subunits acc ... | 2003 | 12534318 |
| combined-modality therapy in locally advanced primary rectal cancer. | patients with unresectable, locally advanced rectal cancer are reported to have a dismal prognosis. the aim of this study was to analyze the effect of combined-modality therapy on clinical outcome. | 2003 | 12544523 |
| human donor bone marrow cells induce in vitro "suppressor t cells" that functionally suppress autologous b cells. | we have reported a beneficial effect of donor vertebral body bone marrow cells (dbmc) infusions in cadaver renal allograft recipients in a 6-year follow-up, but with a transient increase in early (6 month) postoperative cmv infections and concomitant suppressed immunoglobulins (ig) production. we also found that although there was no difference between the dbmc-infused and non-infused (control) groups in the development of donor-specific antibody, we now describe an additional difference seen in ... | 2003 | 12507811 |
| the cell-layer- and cell-type-specific distribution of galnac-transferases in the ocular surface epithelia is altered during keratinization. | it has been hypothesized that the biosynthesis of o-linked glycans on proteins, particularly on the highly o-glycosylated mucins, by the corneal and conjunctival epithelium is necessary for the protection and maintenance of a healthy ocular surface. the initial step in o-glycosylation is the enzymatic addition of n-acetyl galactosamine (galnac) to serine and threonine residues by a large family of polypeptide galnac-transferases (galnac-ts). the purpose of this study was to determine the cellula ... | 2003 | 12506059 |
| analysis of regions within the bacteriophage t4 asia protein involved in its binding to the sigma70 subunit of e. coli rna polymerase and its role as a transcriptional inhibitor and co-activator. | bacteriophage t4 asia, a protein of 90 amino acid residues, binds to the sigma(70) subunit of escherichia coli rna polymerase and inhibits host or t4 early transcription or, together with the t4 mota protein, activates t4 middle transcription. to investigate which regions within asia are involved in forming a complex with sigma(70) and in providing transcriptional functions we generated random mutations throughout asia and targeted mutations within the c-terminal region. we tested mutant protein ... | 2003 | 12527294 |
| guidelines on treatment of stage iiib non-small cell lung cancer. | stage iiib includes patients with t4, any n, m0, and any t, n3, m0. surgery may be indicated only for carefully selected t4n0m0 patients with or without neoadjuvant chemotherapy or chemoradiotherapy. patients with n3 lymph node involvement are not considered as surgical candidates. for patients with unresectable disease, good performance score, and minimal weight loss, treatment with combined chemotherapy and radiotherapy has resulted in better survival than treatment with radiotherapy alone. mu ... | 2003 | 12527581 |
| lung cancer. special treatment issues. | this chapter of the lung cancer guidelines addresses patients with particular forms of non-small cell lung cancer that require special considerations. this includes patients with pancoast tumors, t4n0,1m0 tumors, satellite nodules in the same lobe, synchronous and metachronous multiple primary lung cancers (mplc), and solitary metastases. for patients with a pancoast tumor, a multimodality approach, involving chemoradiotherapy and surgical resection, appears optimal provided appropriate staging ... | 2003 | 12527583 |
| resting thyroid and leptin hormone changes in women following intense, prolonged exercise training. | this study examined whether free (f) triidothyronine (t3), f thyroxine (t4), thyroid stimulating hormone (tsh), and leptin concentrations at rest changed in response to 20 weeks of exercise-training. two groups of women were recruited for participation in the study, collegiate athletes ( n=17) and sedentary controls (n=4). exercise training consisted of daily athletic activity such as rowing, running, and weight lifting. subjects were initially grouped into rowers and controls. however, earlier ... | 2003 | 12527982 |
| endoscopic transthoracic sympathectomy for upper limb hyperhidrosis: limited sympathectomy does not reduce postoperative compensatory sweating. | compensatory sweating is the most common and troublesome complication of thoracodorsal sympathectomy. whether the magnitude of compensatory sweating is related to the extent of sympathectomy is unclear. we investigated the association between the extent of sympathectomy and the occurrence and severity of compensatory sweating after endoscopic transthoracic sympathectomy for upper limb hyperhidrosis. | 2003 | 12514588 |
| mechanism of chaperone function in small heat-shock proteins. phosphorylation-induced activation of two-mode binding in alphab-crystallin. | the consequences of alphab-crystallin phosphorylation on its chaperone activity were investigated using a detailed analysis of the recognition and binding of destabilized t4 lysozyme (t4l) mutants by alphab-crystallin phosphorylation mimics containing combinations of serine to aspartate substitutions. the t4l site-directed mutants were selected to constitute an energetic ladder of progressively destabilized proteins having similar structures in the folded state. alphab-crystallin and its variant ... | 2003 | 12529319 |
| mechanisms underlying the cardiovascular responses to spinal dopamine receptor stimulation by apomorphine in anesthetized rats. | the present study investigated the mechanisms by which intrathecal (i.t.) apomorphine affects mean aortic pressure and heart rate in anesthetized rats. in saline-pretreated rats, upper thoracic (t2-t4) i.t. administration of apomorphine (48 microg/rat) induced immediate and significant hypotension and bradycardia. these responses were unaffected by intravenous (i.v.) methylatropine (1 mg/kg) or bilateral vagotomy, while they were prevented by i.t. lidocaine (25 microl at 1%) or i.v. hexamethoniu ... | 2003 | 12531464 |
| endoscopic ultrasonography in preoperative staging of gastric cancer: determination of tumor invasion depth, nodal involvement and surgical resectability. | current study was aimed to evaluate the usefulness of eus in tnm staging of gastric cancer by comparing eus preoperative staging with pathological findings, and the preliminary exploration of possible reasons for overstaging and understaging phenomenon was especially intended. | 2003 | 12532442 |
| sizing the holin lesion with an endolysin-beta-galactosidase fusion. | double-stranded dna phages require two proteins for efficient host lysis: the endolysin, a muralytic enzyme, and the holin, a small membrane protein. in an event that defines the end of the vegetative cycle, the lambda holin s acts suddenly to permeabilize the membrane. this permeabilization enables the r endolysin to attack the cell wall, after which cell lysis occurs within seconds. a c-terminal fusion of the r endolysin with full-length beta-galactosidase (beta-gal) was tested for lytic compe ... | 2003 | 12533453 |
| escherichia coli endoribonucleases involved in cleavage of bacteriophage t4 mrnas. | the dmd mutant of bacteriophage t4 has a defect in growth because of rapid degradation of late-gene mrnas, presumably caused by mutant-specific cleavages of rna. some such cleavages can occur in an allele-specific manner, depending on the translatability of rna or the presence of a termination codon. other cleavages are independent of translation. in the present study, by introducing plasmids carrying various soc alleles, we could detect cleavages of soc rna in uninfected cells identical to thos ... | 2003 | 12533474 |
| diagnosis of neck recurrences in patients with differentiated thyroid carcinoma. | the follow-up of patients with differentiated thyroid carcinoma (dtc) is traditionally carried out with (131)i whole body scan ((131)i wbs) and serum thyroglobulin (tg) measurement. neck ultrasonography (us) is also used. | 2003 | 12491509 |
| the coenzyme thiamine pyrophosphate inhibits the self-splicing of the group i intron. | effects of the coenzyme thiamine pyrophosphate and its analogs on the inhibition of self-splicing of primary transcripts of the phage t4 thymidylate synthase gene (td) were investigated. of all compounds tested, the coenzyme thiamine pyrophosphate was the most potent inhibitor and the order of inhibitory efficiency for compounds tested was as follows: thiamine pyrophosphate>thiamine monophosphate>thiamine>thiochrome. increasing guanosine concentration overcame the suppression of self-splicing by ... | 2003 | 12479866 |
| chemical activation of cardiac receptors affects activity of superficial and deeper t3-t4 spinal neurons in rats. | the purposes of this study were to examine responses of superficial (depth <300 microm) and deeper thoracic spinal neurons to chemical stimulation of cardiac afferents and effects of descending influences on these neurons. extracellular potentials of single t(3)-t(4) neurons were recorded in pentobarbital anesthetized, paralyzed and ventilated male rats. a catheter was placed in the pericardial sac to administer 0.2 ml of a mixture of algogenic chemicals that contained adenosine (10(-3) m), brad ... | 2003 | 12480160 |
| the tail lysozyme complex of bacteriophage t4. | the tail baseplate of bacteriophage t4 contains a structurally essential, three-domain protein encoded by gene 5 in which the middle domain possesses lysozyme activity. the gene 5 product (gp5) undergoes post-translational cleavage, allowing the resultant n-terminal domain (gp5*) to assemble into the baseplate as a trimer. the lysozyme activity of the undissociated cleaved gp5 is inhibited until infection has been initiated, when the c-terminal portion of the molecule is detached and the rest of ... | 2003 | 12467643 |
| sucrose feeding effects inhibition of gamma-glutamyltranspeptidase in the liver of the rat: possible mediation by thyroid hormone. | the effect of sucrose on fischer 344 rat liver gamma-glutamyltranspeptidase (gammagt) was studied: in adults fed sucrose for 3 weeks; and rats exposed to sucrose from the 18th day of gestation to the 40th day after birth. rats fed regular rodent chow served as controls. sucrose caused mild lipemia; and in the liver an increase in size and fat build-up without damage. in adult sucrose-fed rats, compared to controls, plasma glucose levels were increased: 1.12-, 1.40- and 1.13-fold after 24, 48h an ... | 2003 | 12467647 |
| biochemical characterization of interactions between dna polymerase and single-stranded dna-binding protein in bacteriophage rb69. | the organization and proper assembly of proteins to the primer-template junction during dna replication is essential for accurate and processive dna synthesis. dna replication in rb69 (a t4-like bacteriophage) is similar to those of eukaryotes and archaea and has been a prototype for studies on dna replication and assembly of the functional replisome. to examine protein-protein interactions at the dna replication fork, we have established solution conditions for the formation of a discrete and h ... | 2003 | 12458197 |
| interaction of double-stranded t4 dna with cationic gel of poly(diallyldimethylammonium chloride). | interaction between duplex t4 dna and a slightly cross-linked cationic gel of poly(diallyldimethylammonium chloride) in aqueous media was studied by fluorescent microscopy. while short dna chains such as plasmid dnas penetrate into the gel and form a phase of polyelectrolyte complex with the cationic network, the genomic giant dna chains of t4 phages form complexes only on local areas of the gel surface. the dna/gel complex exhibited different characteristic morphologies depending on the conditi ... | 2003 | 12523843 |
| transoral laser microsurgery for squamous cell carcinoma of the base of the tongue. | to determine the role of transoral laser microsurgery for base of tongue squamous cell carcinoma. | 2003 | 12525192 |
| thyroxine and tri-iodothyronine differently affect uncoupling protein-1 content and antioxidant enzyme activities in rat interscapular brown adipose tissue. | the activity of the antioxidant enzymes copper-zinc superoxide dismutase (cuznsod), manganese superoxide dismutase (mnsod) and catalase (cat), as well as mitochondrial glycerol-3-phosphate dehydrogenase (mgpdh) activity, uncoupling protein-1 (ucp1) content, catecholamine degrading enzyme monoamine oxidase (mao) activity and malonyl dialdehyde (mda) concentration were studied in rat interscapular brown adipose tissue (ibat). rats were treated with either thyroxine (t4) or tri-iodothyronine (t3) f ... | 2003 | 12525247 |
| primary chemotherapy in resectable oral cavity squamous cell cancer: a randomized controlled trial. | prognosis of patients with advanced oral cavity cancer is worth improving. chemotherapy has been reported to be especially active in oral cavity tumors. here we repeat the results of a randomized, multicenter trial enrolling patients with a resectable, stage t2-t4 (> 3 cm), n0-n2, m0 untreated, squamous cell carcinoma of the oral cavity. | 2003 | 12525526 |
| response to preoperative chemoradiation increases the use of sphincter-preserving surgery in patients with locally advanced low rectal carcinoma. | although controversial, some believe that preoperative chemoradiation increases the use of sphincter-preserving surgery in low rectal carcinoma patients. this article investigates the relationship between objective tumor response and sphincter preservation in low rectal carcinoma patients. | 2003 | 12518377 |
| a population-based study of thyroid function after radiotherapy and chemotherapy for a childhood brain tumor. | the effect of craniospinal irradiation (csi) vs. cranial irradiation (cir) only with or without chemotherapy (ct) on the hypothalamus/pituitary (hp) thyroid axis was assessed in a population-based study of patients treated for a childhood brain tumor not directly involving the hp axis. thyroid function was evaluated and compared with that in healthy controls (n = 27), measuring tsh, free t4, total t4, total t3, and trh. the biological effective dose (bed) of radiotherapy, determined for the hp r ... | 2003 | 12519842 |
| cardiovascular safety of acute recombinant human thyrotropin administration to patients monitored for differentiated thyroid cancer. | eleven patients who had undergone total thyroidectomy for differentiated thyroid cancer and who were on chronic tsh-suppressive therapy with levothyroxine (l-t4), underwent 24-h holter electrocardiogram and doppler-echocardiography before and after acute recombinant human tsh (rhtsh) administration for disease staging. the treatment, which was generally well tolerated, did not affect circulating thyroid hormones levels, nor did it have measurable effects on heart rate, rhythm, left ventricular m ... | 2003 | 12519854 |
| luteal phase deficiency in recreational runners: evidence for a hypometabolic state. | exercising women with amenorrhea exhibit a hypometabolic state. the purpose of this study was to evaluate the relationship of luteal phase deficient (lpd) menstrual cycles to metabolic hormones, including thyroid, insulin, human gh (hgh), leptin, and igf-i and its binding protein levels in recreational runners. menstrual cycle status was determined for three consecutive cycles in sedentary and moderately active women. menstrual status was defined as ovulatory or lpd. subjects were either sedenta ... | 2003 | 12519874 |
| acute phase effects of atp-mgcl2 on experimental spinal cord injury. | the purpose of this study was to study the acute phase effects of adenosine triphosphate (atp)-mgcl2 on experimental spinal cord clip compression injury. spinal cord clip compression injury was performed on 36 albino wistar rats. the rats were divided into five groups. t4-t8 total laminectomy was performed on all rats. group 1: sham-operated group. group 2: clip compression group. in group 3, atp-mgcl2 (100 micro mol/kg) was given 2 min before the "clip compression injury." in group 4, atp-mgcl2 ... | 2003 | 12520320 |
| complementation of bacteriophage induction and recombination defects in escherichia coli reca(-) mutants by expression of the cloned t4 bacteriophage uvsx gene. | previous workers reported that the t4 bacteriophage uvsx protein could promote neither reca-lexa-mediated dna repair nor induction of lysogenized bacteriophage, only recombination. reexamination of these phenotypes demonstrated that, in contrast to these prior studies, when this gene was cloned into a medium but not a low-copy-number vector, it stimulated both a high frequency of spontaneous induction and mitomycin c-stimulated bacteriophage induction in a strain containing a reca13 mutation, bu ... | 2003 | 12520361 |
| mutational and functional analysis of a segment of the sigma family bacteriophage t4 late promoter recognition protein gp55. | bacteriophage t4 late promoters, which consist of a simple 8-base pair tata box, are recognized by the gene 55 protein (gp55), a small, highly diverged member of the sigma family proteins that replaces sigma(70) during the final phase of the t4 multiplication cycle. a 16-amino acid segment of gp55 that is proposed to be homologous to the sigma(70) region 2.2 has been subjected to alanine scanning and other mutagenesis. the corresponding proteins have been examined in vitro for binding to escheri ... | 2003 | 12496274 |
| bacteriophage t4 dam dna-(n6-adenine)-methyltransferase. processivity and orientation to the methylation target. | we carried out steady state and pre-steady state (burst) kinetic analyses of the bacteriophage t4 dam dna-(n(6)-adenine)-methyltransferase (mtase)-mediated methyl group transfer from s-adenosyl-l-methionine (adomet) to ade in oligonucleotide duplexes containing one or two specific gatc sites with different combinations of methylated and unmodified targets. we compared the results for ligated 40-mer duplexes with those of the mixtures of the two unligated duplexes used to generate the 40-mers. th ... | 2003 | 12501249 |
| sites of local recurrence after surgery, with or without chemotherapy, for rectal cancer: implications for radiotherapy field design. | to examine the sites of pelvic recurrence in patients with rectal cancer previously untreated with radiotherapy to determine the relative frequency and location of recurrence within the pelvis. | 2003 | 12504046 |
| time course of gag protein assembly in hiv-1-infected cells: a study by immunoelectron microscopy. | recent biochemical studies have identified high molecular complexes of the hiv gag precursor in the cytosol of infected cells. using immunoelectron microscopy we studied the time course of the synthesis and assembly of a hiv gag precursor protein (pr55gag) in sf9 cells infected with recombinant baculovirus expressing the hiv gag gene. we also immunolabeled for pr55gag human t4 cells acutely or chronically infected with hiv-1. in sf9 cells, the time course study showed that the first gag protein ... | 2003 | 12504555 |
| shortened irradiation scheme, continuous infusion of 5-fluorouracil and fractionation of mitomycin c in locally advanced anal carcinomas. results of a phase ii study of the european organization for research and treatment of cancer. radiotherapy and gastrointestinal cooperative groups. | the european organization for research and treatment of cancer (eortc) 22861 randomised trial established that combined radiochemotherapy is the standard treatment for locally advanced anal cancer. this eortc phase ii study (#22953) tests the feasibility of reducing the gap between sequences to 2 weeks, to deliver mitomycin c (mmc) in each radiotherapy sequence and 5-fu continuously during the treatment. the first sequence consisted of 36 gy over 4 weeks. 5-fu 200 mg/m(2)/days 1-26, mmc 10 mg/m( ... | 2003 | 12504657 |
| multiple methionine substitutions are tolerated in t4 lysozyme and have coupled effects on folding and stability. | in order to further explore the tolerance of proteins to amino acid substitutions within the interior, a series of core residues was replaced by methionine within the c-terminal domain of t4 lysozyme. by replacing leucine, isoleucine, valine and phenylalanine residues a total of 10 methionines could be introduced, which corresponds to a third of the residues that are buried in this domain. as more methionines are incorporated the protein gradually loses stability. this is attributed in part to a ... | 2003 | 12646375 |
| surgical therapy in hashimoto's thyroiditis. | hashimoto's thyroiditis (ht) is usually considered to be manageable by levothyroxine (l-t4) administration, which can reduce the thyroid volume and supplement the lack of hormone. however, we sometimes encounter a huge goiter that has not shrunk in response to l-t4 therapy. these goiters continue to produce symptoms of compression and an unsightly appearance. here we discuss the surgical indication and procedure for ht. thirteen patients with clinically diagnosed hashimoto's thyroiditis involvin ... | 2003 | 12646974 |
| rna chaperone activity of the sm-like hfq protein. | the escherichia coli sm-like host factor i (hfq) protein is thought to function in post-transcriptional regulation by modulating the function of small regulatory rnas. hfq also interferes with ribosome binding on e. coli ompa messenger rna, indicating that hfq also interacts with mrnas. in this study, we have used stimulation of group i intron splicing in vivo and a modified in vitro toeprinting assay to determine whether hfq acts as an rna chaperone. hfq was able to rescue an rna 'folding trap' ... | 2003 | 12634847 |
| immunohistochemical expression of c-reactive protein in squamous cell carcinoma of the esophagus - significance as a tumor marker. | c-reactive protein (crp) is a prototype acute phase protein which has been known to be synthesized in hepatocytes. although serum elevation of crp has been reported to be an indicator of the unfavorable outcome of the patients in some malignant tumors, the expression of the protein in carcinoma cells has not been investigated. the aim of the current study was to assess the immunohistochemical expression of crp in squamous cell carcinoma (scc) of the esophagus and to find its biological significa ... | 2003 | 12637157 |
| pseudo-central hypothyroidism. | central hypothyroidism is an exceedingly rare form of hypothyroidism that results from a variety of conditions affecting the hypothalamus and the pituitary gland. the classic biochemical abnormality seen in these patients includes a low serum level of circulating thyroxine (t4) concomitant with an inappropriately low level of thyrotropin. because patients with isolated triiodothyronine (t3) toxicosis also present with this biochemical pattern, it is important to measure t3 levels in such patient ... | 2003 | 12630650 |
| sensitive time-resolved fluoroimmunoassay for simultaneous detection of serum thyroid-stimulating hormone and total thyroxin with eu and sm as labels. | based on a novel cocoating strategy and dissociation enhancement lanthanide fluorescence immunoassay technique, a sensitive time-resolved fluoroimmunoassay (trfia) has been developed for simultaneous quantification of human serum thyroid-stimulating hormone (tsh) and thyroxin (t4) in a one-and-the-same assay procedure. the new cocoating strategy for preparing highly active surface anti-tsh and anti-t4 monoclonal antibodies (mcabs) was performed by a three-step protocol. namely, anti-tsh mcab at ... | 2003 | 12633606 |
| kinetic regulation of single dna molecule denaturation by t4 gene 32 protein structural domains. | bacteriophage t4 gene 32 protein (gp32) specifically binds single-stranded dna, a property essential for its role in dna replication, recombination, and repair. although on a thermodynamic basis, single-stranded dna binding proteins should lower the thermal melting temperature of double-stranded dna (dsdna), gp32 does not. using single molecule force spectroscopy, we show for the first time that gp32 is capable of slowly destabilizing natural dsdna. direct measurements of single dna molecule den ... | 2003 | 12634053 |
| blocking-type anti-tsh receptor antibodies and relation to responsiveness to antithyroid drug therapy and remission in graves' disease. | antithyroid drugs are effective in some patients with graves' disease but not in others. the factors responsible for this difference are still unknown. we examined the relationship between the nature of anti-tsh receptor (tsh-r) antibodies and responsiveness to drugs in graves' disease. | 2003 | 12641621 |
| two novel mutations in the gene encoding thyroxine-binding globulin (tbg) as a cause of complete tbg deficiency in taiwan. | thyroxine-binding globulin (tbg) encoded by the tbg gene on chromosome xq22 is the major transport protein, carrying approximately 75% of circulating t4. inherited defects in tbg are associated with three phenotypes based on the level of tbg in serum of affected hemizygous males: complete tbg deficiency (tbg-cd), partial tbg deficiency (tbg-pd) and tbg excess (tbg-e). in this study, we report two unrelated han chinese males with complete tbg deficiency who carry different mutations in the tbg ge ... | 2003 | 12641622 |
| effects of therapeutic doses of 131i in thyroid papillary carcinoma patients with elevated thyroglobulin level and negative 131i whole-body scan: comparative study. | previous studies have shown a high rate of visualization of uptake and a decrease in serum thyroglobulin (tg) after therapeutic doses of 131i in well-differentiated thyroid cancer patients with elevated thyroglobulinaemia but negative diagnostic 131i whole-body scan (dxwbs), but its therapeutic effect remains controversial. we evaluate the effect of therapeutic doses of 131i in patients with elevated thyroglobulin level but negative dxwbs. | 2003 | 12641624 |
| preoperative lanreotide treatment for gh-secreting pituitary adenomas: effect on tumour volume and predictive factors of significant tumour shrinkage. | the objective of this open study of 104 patients was to determine whether the somatostatin analogue lanreotide shrinks gh-secreting adenomas and to identify the predictive factors of a significant tumour volume reduction (> 20%). | 2003 | 12641631 |
| type 2 deiodinase expression is stimulated by growth factors in human vascular smooth muscle cells. | type 2 deiodinase (d2) catalyzes the conversion of the prohormone t4 to the biologically active t3. d2 is expressed in human aortic smooth muscle cells (hasmcs). in this study, we demonstrated that the d2 mrna and activity in hasmcs were up-regulated by platelet-derived growth factor-bb (pdgf-bb) and basic fibroblast growth factor (bfgf). the induction of d2 mrna by pdgf-bb and bfgf was dependent on de novo rna and protein synthesis. pd98059, a specific inhibitor of the upstream kinase that acti ... | 2003 | 12644304 |
| monitoring of neuromuscular block after administration of vecuronium in patients with diabetes mellitus. | we studied the supramaximal current for ulnar nerve stimulation during electromyographic monitoring of onset and recovery of neuromuscular block using a neuromuscular transmission module (m-nmt module, datex-ohmeda) in patients with type 2 diabetes undergoing anaesthesia with nitrous oxide, oxygen, isoflurane and fentanyl. | 2003 | 12644421 |
| thyroid hormone production rates in rat liver and intestine in vivo: a novel graph theory and experimental solution. | we develop a novel method for finding sufficient experimental conditions for discriminating and quantifying individual biomolecule production sources in distributed, inhomogeneous multisource systems in vivo, and we apply it experimentally to a complex, unsolved problem in endocrinology. the majority of hormonal triiodothyronine (t3) is produced from prohormone thyroxine (t4) in numerous nonthyroidal organs and, with one exception, the t3 production rate has not been fully resolved in any single ... | 2003 | 12644447 |
| 3'-exonuclease resistance of dna oligodeoxynucleotides containing o6-[4-oxo-4-(3-pyridyl)butyl]guanine. | tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk), is a chemical carcinogen thought to be involved in the initiation of lung cancer in smokers. nnk is metabolically activated to methylating and pyridyloxobutylating species that form promutagenic adducts with dna nucleobases, e.g. o(6)-[4-oxo-4-(3-pyridyl)butyl]guanine (o(6)-pob-dg). o(6)-pob-dg is a strongly mispairing dna lesion capable of inducing both g-->a and g-->t base changes, suggesting its importance in ... | 2003 | 12655016 |
| internal 32p-labeling of l-deoxyoligonucleotides. | a general two step procedure for the internal labeling of l-deoxyoligonucleotides, spiegelmers, has been developed. through radioactive labeling oligonucleotides can easily be detected and monitored in biological samples. t4 polynucleotide kinase is shown to efficiently phosphorylate strands of l-nucleic acids which allows the labeling with phosphorous isotopes such as (32)p. in order to protect the terminal phosphate label against unspecific phosphatases, one of two fragments of a spiegelmer is ... | 2003 | 12655022 |