Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| The Nature of an In Vivo Anti-Capsular Polysaccharide Response Is Markedly Influenced by the Composition and/or Architecture of the Bacterial Subcapsular Domain. | In vivo anti-polysaccharide Ig responses to isolated polysaccharide (PS) are T cell independent, rapid, and fail to generate memory. However, little is known regarding PS-specific Ig responses to intact Gram-positive and Gram-negative extracellular bacteria. We previously demonstrated that intact heat-killed Streptococcus pneumoniae, a Gram-positive bacterium, elicited a rapid primary pneumococcal capsular PS (PPS) response in mice that was dependent on CD4(+) T cells, B7-dependent costimulation ... | 2011 | 22156342 |
| Phasevarion Mediated Epigenetic Gene Regulation in Helicobacter pylori. | Many host-adapted bacterial pathogens contain DNA methyltransferases (mod genes) that are subject to phase-variable expression (high-frequency reversible ON/OFF switching of gene expression). In Haemophilus influenzae and pathogenic Neisseria, the random switching of the modA gene, associated with a phase-variable type III restriction modification (R-M) system, controls expression of a phase-variable regulon of genes (a "phasevarion"), via differential methylation of the genome in the modA ON an ... | 2011 | 22162751 |
| phase i study of a neisseria meningitidis liposomal vaccine containing purified outer membrane proteins and detoxified lipooligosaccharide. | purified outer membrane proteins and purified deacylated lipooligosaccharide (dlos) were formulated for use as a vaccine in three formulations for clinical use. the three vaccine formulations included (1) purified outer membrane proteins (omps) and l8-5 dlos adsorbed to aluminum hydroxide; (2) purified omps and l8-5 dlos incorporated into liposomes; and (3) purified omps and l7 dlos incorporated into proteoliposomes. the vaccines were compared for immunogenicity and safety in a phase 1clinical s ... | 2011 | 22138211 |
| Efficacy of a capsule conjugate vaccine against inhalational anthrax in rabbits and monkeys. | Bacillus anthracis, the causative agent of anthrax, is recognized as one of the most serious bioterrorism threats. The current human vaccines are based on the protective antigen component of the anthrax toxins. Concern about possible vaccine resistant strains and reliance on a single antigen has prompted the search for additional immunogens. Bacterial capsules, as surface-expressed virulence factors, are well-established components of several licensed vaccines. In a previous study we showed that ... | 2011 | 22172509 |
| Targeting of Neisserial PorB to the mitochondrial outer membrane: an insight on the evolution of ß-barrel protein assembly machines. | Mitochondria originated from Gram-negative bacteria through endosymbiosis. In modern day mitochondria, the Sorting and Assembly Machinery (SAM) is responsible for eukaryotic ß-barrel protein assembly in the mitochondrial outer membrane. The SAM is the functional equivalent of the ß-barrel assembly machinery found in the outer membrane of Gram-negative bacteria. In this study we examined the import pathway of a pathogenic bacterial protein, PorB, which is targeted from pathogenic Neisseria to the ... | 2011 | 22032638 |
| A novel metal transporter mediating manganese export (MntX) regulates the Mn to Fe intracellular ratio and Neisseria meningitidis virulence. | Neisseria meningitidis (Nm) and N. gonorrhoeae (Ng) are adapted to different environments within their human host. If the basis of this difference has not yet been fully understood, previous studies (including our own data) have reported that, unlike Ng, Nm tolerates high manganese concentrations. As transition metals are essential regulators of cell growth and host pathogen interactions, we aimed to address mechanisms of Nm Mn²? tolerance and its pathogenic consequences. Using bioinformatics, g ... | 2011 | 21980287 |
| Differing Neisseria gonorrhoeae Bacterial Loads in the Pharynx and Rectum in Men Who Have Sex with Men: Implications for Gonococcal Detection, Transmission, and Control. | The bacterial loads for gonococcal infections of the pharynx and rectum were determined among men with male sexual partners. The median bacterial load for rectal infections (18,960 copies/swab) was significantly higher than the load for pharyngeal infections (2,100 copies/swab; P = 0.001). Bacterial loads among men with symptomatic proctitis were strikingly high (median, 278,000 copies/swab). | 2011 | 21956992 |
| scent recognition of infected status in humans. | introduction. there is a body of experimental evidence that mice and rats use chemical signals to avoid sexual contact with infected conspecifics. in contrast to animals, body scent of sick humans is employed only in medical diagnostics. a modification of human body odor, due to an infection, has not been studied as a potential signal for choice of a sexual partner. it might, however, be especially important for sexually transmitted infections (sti) because many such infections have no obvious ... | 2011 | 22145868 |
| changes in serogroup and genotype prevalence among carried meningococci in the united kingdom during vaccine implementation. | herd immunity is important in the effectiveness of conjugate polysaccharide vaccines against encapsulated bacteria. a large multicenter study investigated the effect of meningococcal serogroup c conjugate vaccine introduction on the meningococcal population. | 2011 | 21881120 |
| lps from neisseria meningitidis is crucial for inducing monocyte- and microparticle-associated tissue factor activity but not for tissue factor expression. | neisseria meningitidis causes sepsis with coagulopathy. the present study evaluated the tissue factor (tf)-inducing capacity of bacterial lps in different presentation forms, i.e. membrane-bound lps versus purified lps, and of non-lps components of n. meningitidis. by using a wild-type n. meningitidis, a mutant n. meningitidis lacking lps (lps-deficient n. meningitidis), purified lps from n. meningitidis and escherichia coli, we measured tf-expression and tf-activity on human monocytes and micro ... | 2011 | 22180561 |
| Distribution of serogroups and sequence types in disease-associated and carrier strains of Neisseria meningitidis isolated in China between 2003 and 2008. | SUMMARYGiven the unpredictability of Neisseria meningitidis outbreaks and the increased prevalence of serogroup C strains following the introduction of serogroup A-based vaccines, we conducted an analysis of serogroups and sequence types (STs) in disease-associated and carrier N. meningitidis isolates that have emerged in China since 2003. We used multilocus sequence-typing techniques to investigate 371 N. meningitidis strains isolated from patients with meningitis and healthy carriers. Two line ... | 2011 | 21929839 |
| adolescent female with urinary symptoms: a diagnostic challenge for the pediatrician. | the objective of this study was to determine the accuracy with which physicians diagnose sexually transmitted infections (stis) and urinary tract infections (utis) in adolescent females with urinary symptoms. secondary aims were to determine the prevalence of stis and utis in this subset of patients and to identify variables associated with a physician diagnosis of sti or uti. | 2011 | 21878833 |
| conjugate meningococcal vaccines development: gsk biologicals experience. | meningococcal diseases are serious threats to global health, and new vaccines specifically tailored to meet the age-related needs of various geographical areas are required. this paper focuses on the meningococcal conjugate vaccines developed by gsk biologicals. two combined conjugate vaccines were developed to help protect infants and young children in countries where the incidence of meningococcal serogroup c or serogroup c and y disease is important: hib-menc-tt vaccine, which offers protecti ... | 2011 | 21991444 |
| Comparing Cerebrospinal Fluid Values in newbornsand infants until two months old with or without meningitis." | Background/Objective: To evaluate the characteristics and accuracy of cerebrospinal fluid (CSF) values of neonatal meningitis, we compared CSF values in newborns and infants until 2 months old with or without meningitis. Methods. This case-control study was performed on 120 newborns and infants until 2 months old. 60 patients with meningitis were considered as case group and 60 suspected meningitis were as control group. Each of two groups was divided into 0-1 months and 1- 2 months old. CSF* ... | 2011 | 22192569 |
| Porphyromonas gingivalis: a clonal pathogen?: Diversities in housekeeping genes and the major fimbriae gene. | The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been established at www.pubmlst.org/pgingivalis, and data from the database indicate a high degree of genetic diversi ... | 2011 | 22125739 |
| Structure of the C-terminal Domain of Neisseria Heparin Binding Antigen (NHBA), One of the Main Antigens of a Novel Vaccine against Neisseria meningitidis. | Neisseria heparin binding antigen (NHBA), also known as GNA2132 (genome-derived Neisseria antigen 2132), is a surface-exposed lipoprotein from Neisseria meningitidis that was originally identified by reverse vaccinology. It is one the three main antigens of a multicomponent vaccine against serogroup B meningitis (4CMenB), which has just completed phase III clinical trials in infants. In contrast to the other two main vaccine components, little is known about the origin of the immunogenicity of t ... | 2011 | 21965688 |
| pre-hospital antibiotics for meningococcal disease remains low. | 2011 | 21964019 | |
| From agonist to antagonist: structure and dynamics of innate immune glycoprotein MD-2 upon recognition of variably acylated bacterial endotoxins. | The human immune response to an infection by Gram-negative bacteria involves detection of lipopolysaccharides (LPS), also known as endotoxins, which comprise the bacterial outer cell wall. Distinct from mammalian glycolipid structures, LPS have a conserved chemical pattern that is recognized by the pattern recognition receptor complex formed by myeloid differentiation protein 2 (MD-2) and toll-like receptor 4 (TLR4). A remarkable immune-mediated structure-toxicity relationship has been defined t ... | 2011 | 21924775 |
| safety, immunogenicity and preliminary efficacy of multiple-site vaccination with an epidermal growth factor (egf) based cancer vaccine in advanced non small cell lung cancer (nsclc) patients. | abstract: the prognosis of patients with advanced non small cell lung (nsclc) cancer remains dismal. epidermal growth factor receptor is over-expressed in many epithelial derived tumors and its role in the development and progression of nsclc is widely documented. cimavax-egf is a therapeutic cancer vaccine composed by human recombinant epidermal growth factor (egf) conjugated to a carrier protein, p64k from neisseria meningitides. the vaccine is intended to induce antibodies against self egf th ... | 2011 | 22024351 |
| post-genomics of neisseria meningitidis: an update. | neisseria meningitidis infection still remains a major life-threatening bacterial disease worldwide. the availability of bacterial genomic sequences generated a paradigm shift in microbiological and vaccines sciences, and post-genomics (comparative genomics, functional genomics, proteomics and a combination/evolution of these techniques) played important roles in elucidating bacterial biological complexity and pathogenic traits, at the same time accelerating the development of therapeutic drugs ... | 2011 | 22087663 |
| Acute bacterial meningitis in infants and children: epidemiology and management. | Acute bacterial meningitis (ABM) continues to be associated with high mortality and morbidity, despite advances in antimicrobial therapy. The causative organism varies with age, immune function, immunization status, and geographic region, and empiric therapy for meningitis is based on these factors. Haemophilus influenzae type b (Hib), Streptococcus pneumoniae, and Neisseria meningitidis cause the majority of cases of ABM. Disease epidemiology is changing rapidly due to immunization practices an ... | 2011 | 21999651 |
| [Early diagnosis and correlations of sexually transmitted infections among women in primary care health services]. | Sexually Transmitted Infections (STIs) in women remain a public health challenge due to high prevalence, difficulties to implement early diagnosis strategies and high rates of complications. | 2011 | 22069014 |
| Factors associated with HIV among female sex workers in a high HIV prevalent state of India. | Abstract The study was carried out to assess the factors associated with HIV seropositivity among female sex workers (FSWs) in Dimapur, Nagaland, a high HIV prevalence state of India. A total of 426 FSWs were recruited into the study using respondent driven sampling (RDS). Data on demographic characteristics, sexual and injecting risk behaviours were collected from them and were tested for HIV, Syphilis, Neisseria gonorrhoeae and Chlamydia trachomatis. RDS-weighted univariate and multivariate lo ... | 2011 | 21902571 |
| Meningococcal vaccines: WHO position paper, November 2011. | 2011 | 22128384 | |
| Cloning, expression and purification of outer membrane protein PorA of Neisseria meningitidis serogroup B. | Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N. meningitidis serogroup B. PorA is a major component of the outer membrane of N. meningitidis and functions as a cationic porin. This study aimed to clone and determine the expression of PorA. | 2011 | 22169784 |
| Sustainable antimicrobial surveillance programs essential for controlling Neisseria gonorrhoeae superbug. | 2011 | 21934559 | |
| Are we satisfied with the tools for the diagnosis of gonococcal infection in females? | Gonorrhea (Neisseria gonorrhoeae) is a common sexually transmitted infection in women, with a heavy burden on female and neonatal health, because sequelae occur, such as female infertility, ectopic pregnancy, neonatal ophthalmitis and infection, and chronic pelvic pain. Prompt and appropriate antibiotic treatment can cure infection and avoid complications. However, adequate treatment is not easy, because early and rapid identification of gonorrhea is interfered with by many factors, including th ... | 2011 | 22036133 |
| core groups, antimicrobial resistance and rebound in gonorrhoea in north america. | backgroundgenital tract infections caused by neisseria gonorrhoeae are a major cause of sexually transmitted disease worldwide. surveillance data suggest that incidence has increased in recent years after initially falling in the face of intensified control efforts.objectivesthe authors sought to evaluate the potential contribution of antimicrobial resistance to such rebound and to identify optimal treatment strategies in the face of resistance using a mathematical model of gonorrhoea.methodsthe ... | 2011 | 22169277 |
| biofouling characteristics and identification of preponderant bacteria at different nutrient levels in batch tests of a recirculating cooling water system. | understanding the influence of nutrient levels on biofouling control is an important requirement for management strategies in a recirculating cooling water system. nutrient limitation may be one way to control biofouling development without increasing biocide dosing. therefore, this study was carried out to investigate the effects of nutrient levels on biofouling characteristics and to identify the preponderant bacteria in the batch tests with a simulated cooling water system. the biofouling cha ... | 2011 | 21879565 |
| [Nationwide antimicrobial susceptibility survey of Neisseria gonorrhoeae isolates in Japan]. | In a nationwide antimicrobial susceptibility survey of 494 Nesseria gonorrhoeae isolates collected from February 2008 to December 2009 in 3 regions of Japan, 112 (22.7%) were collected from western Japan (Kinki, Chugoku, Shikoku, and Kyushu), 277 (56.1%) from mid-eastern Japan (Kanto), and 105 (21.1%) from eastern Japan (Tokai, Hokuriku, Koushinetsu, Tohoku, and Hokkaido). Resistance to ciprofloxacin (CPFX) was 72.8%, to penicillin G (PCG) 19.8%, and to tetracycline (TC) 18.2%. Intermediate resi ... | 2011 | 21861439 |
| the utility and cost of chlamydia trachomatis and neisseria gonorrhoeae screening of a male infertility population. | objective: to determine the utility and cost of chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) screening in infertile males. design: cohort study. setting: canadian tertiary-level male infertility clinic and university-affiliated laboratories. patient(s): 5,588 male infertility patients. intervention(s): ct and ng testing on 8,972 urine and semen samples. main outcome measure(s): prevalence of ct and ng infection in infertile males versus general male population in canada over 8 years ... | 2011 | 22192351 |
| enhanced bacteremia in human factor h transgenic rats infected by neisseria meningitidis. | neisseria meningitidis binds the complement down-regulating protein, factor h (fh), which enables the organism to evade host defenses. two fh ligands, fhbp and nspa, are known to bind specifically to human fh. we developed a human fh transgenic infant rat model to investigate the effect of human fh on meningococcal bacteremia. 18 hours after ip challenge with 560 cfu of group b strain h44/76, all 19 human fh-positive rats had positive blood cultures compared with 0 of 7 human fh-negative control ... | 2011 | 22104107 |
| neisserial correia repeat-enclosed elements do not influence the transcription of pil genes in neisseria gonorrhoeae and neisseria meningitidis. | two human-specific neisserial pathogens, neisseria gonorrhoeae and neisseria meningitidis, require the expression of type iv pili (tfp) for initial attachment to the host during infection. however, the mechanisms controlling the assembly and functionality of tfp are poorly understood. it is known that the gonococcal pile gene, encoding the major subunit, is positively regulated by ihf, a multifunctional dna binding protein. a neisserial specific repetitive dna sequence, termed the correia repeat ... | 2011 | 21856854 |
| nonhuman primate models used to study pelvic inflammatory disease caused by chlamydia trachomatis. | pelvic inflammatory disease (pid) is a global health concern that is associated with significant morbidity and is a major cause of infertility. throughout history animals have been used for anatomical studies and later as models of human disease. in particular, nonhuman primates (nhps) have permitted investigations of human disease in a biologically, physiologically, and anatomically similar system. the use of nhps as human pid models has led to a greater understanding of the primary microorgani ... | 2011 | 21869858 |
| epidemiology of invasive meningococcal disease in austria 2010. | neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. thus, monitoring the epidemiology of invasive meningococcal disease (imd) is an important public health measure. the national reference centre for meningococci at the austrian agency for health and food safety is operating the laboratory-based surveillance database. | 2011 | 21935643 |
| immediate outcomes of bacterial meningitis in childhood may benefit from slow initial β-lactam infusion and oral paracetamol. | evaluation of: pelkonen t, roine i, cruzeiro ml, pitkaranta a, kataja m, peltola h. slow initial β-lactam infusion and oral paracetamol to treat childhood bacterial meningitis: a randomised, controlled trial. lancet infect. dis. 11(8), 613-621 (2011). acute bacterial meningitis is a medical emergency that requires prompt management. despite effective antibiotic and adjunctive therapies, mortality is still unacceptably high in acute bacterial meningitis in children as this mortality did not subst ... | 2011 | 22004030 |
| cleavage of von willebrand factor by granzyme m destroys its factor viii binding capacity. | von willebrand factor (vwf) is a pro-hemostatic multimeric plasma protein that promotes platelet aggregation and stabilizes coagulation factor viii (fviii) in plasma. the metalloproteinase adamts13 regulates the platelet aggregation function of vwf via proteolysis. severe deficiency of adamts13 is associated with thrombotic thrombocytopenic purpura, but does not always correlate with its clinical course. therefore, other proteases could also be important in regulating vwf activity. in the presen ... | 2011 | 21909423 |
| cutaneous gonococcal abscess of the abdomen in a child. | virtually all pediatric cases of neisseria gonorrhoeae originate from contact with an infected adult. a cutaneous abscess caused by n. gonorrhoeae in a child is extremely rare, especially outside the genital area. we report a case of a 22-month-old boy with a gonococcal cutaneous abscess on the abdominal wall and suggest that n. gonorrhoeae should be included in the differential diagnosis of skin and soft tissue infections in children. | 2011 | 21926887 |
| a snapshot of a pathogenic bacterium mid-evolution: neisseria meningitidis is becoming a nitric oxide-tolerant aerobe. | members of the neisseria genus typically display the ability to carry out denitrification of nitrite to nitrous oxide as an alternative to oxygen respiration when oxygen is depleted. the key enzymes nitrite and nitric oxide reductase are found across the neisseria genus. within neisseria meningitidis, however, a number of research groups have found that a significant proportion of strains lack a functional nitrite reductase. it appears that n. meningitidis is on an evolutionary trajectory toward ... | 2011 | 22103546 |
| neisseria base: a comparative genomics database for neisseria meningitidis. | neisseria meningitidis is an important pathogen, causing life-threatening diseases including meningitis, septicemia and in some cases pneumonia. genomic studies hold great promise for n. meningitidis research, but substantial database resources are needed to deal with the wealth of information that comes with completely sequenced and annotated genomes. to address this need, we developed neisseria base (nbase), a comparative genomics database and genome browser that houses and displays publicly a ... | 2011 | 21930505 |
| vaccine-preventable diseases and their impact on latin american children. | a joint meeting of the latin american society of pediatric infectious diseases, the dominican society of pediatrics and the dominican society of vaccinology was held in the dominican republic. this report highlights the most relevant issues that were presented and discussed about vaccine-preventable diseases, their epidemiology and impact in latin american children, the need to move forward and expand national immunization programs and the economical and political obstacles to introduce ?new? va ... | 2011 | 22085169 |
| genotyping of two neisseria gonorrhoeae fluroquinolone-resistant strains in the brazilian amazon region. | we report two ciprofloxacin and ofloxacin-resistant neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the alfredo da matta foundation (manaus, state of amazonas, brazil). the gonococci displayed minimal inhibitory concentrations (> 32.00 µg/ml) and three mutations in the quinolone resistance-determining region (s91f and d95g in gyra and s87r in parc). both isolates were genotyped using n. gonorr ... | 2011 | 21894387 |
| molecular analyses of tem genes and their corresponding penicillinase-producing neisseria gonorrhoeae isolates in bangkok, thailand. | neisseria gonorrhoeae is a major public health problem globally, especially because the bacterium has developed resistance to most antimicrobials introduced for first-line treatment of gonorrhea. in the present study, 96 n. gonorrhoeae isolates with high-level resistance to penicillin from 121 clinical isolates in thailand were examined to investigate changes related to their plasmid-mediated penicillin resistance and their molecular epidemiological relationships. a β-lactamase (tem) gene varian ... | 2011 | 22143532 |
| lower genital tract infections in hiv-seropositive women in india. | the presence of std facilitates shedding of hiv and increases hiv-1 disease progression, possibly by increasing plasma viremia. our aim was to study the presence of various associated sexually transmitted disease/reproductory tract infections in hiv-seropositive women in india. | 2011 | 22021972 |
| [meningitis and encephalitis in poland in 2009]. | in poland, 2 517 cases of neuroinfections were reported in 2009, of which 865 had bacterial aetiology, 1 244--viral aetiology, and 408-- other or unknown origin. the etiological agent was determined in 493 (57%) cases of bacterial neuroinfections. among them neisseria meningitidis was found in 190 cases, haemophilus influenzae type b (hib) in 13 cases and streptococcus pneumoniae in 163 cases. an increasing trend in pneumococcal infections incidence has been observed since 2005, and a substantia ... | 2011 | 21913462 |
| elevated soluble urokinase receptor values in csf, age and bacterial meningitis infection are independent and additive risk factors of fatal outcome. | the aim of the present study was to evaluate the potential role of cerebrospinal fluid soluble urokinase receptor (supar) level, infection and age as risk factors for fatal outcome in patients suspected of having meningitis and/or bacteraemia on admission to hospital. a total of 545 cerebrospinal fluid samples from patients with clinically suspected meningitis were sent to the hellenic national meningitis reference laboratory. ten of 545 (1.83%) patients died. analysis by receiver operating char ... | 2011 | 21971819 |
| [severe bacterial infections and sudden death in children less than 4-years-old: 3 case reports.] | infectious factors contribute to sudden infant death in about 1 case out of 3. we report 3 children less than 4-years-old who died suddenly of bacterial infection due to neisseria meningitidis, streptococcus pneumoniae, and staphylococcus aureus. the bacteria were isolated from central and peripheral samples. a bacterial origin should be sought in all cases of sudden death in children. when a bacterial origin is confirmed, the question of immunodeficiency should be raised. | 2011 | 22209380 |
| evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals. | the aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals. | 2011 | 21864643 |
| evaluation of predominant neisseria gonorrhoeae strain types and its correlation with fluoroquinolone resistance in pakistan. | to study the predominant neisseria gonorrhoeae strain types in pakistan and to evaluate their correlation with fluoroquinolone resistance. | 2011 | 22204176 |
| sexually transmitted infections and risk behaviors among african american women who have sex with women: does sex with men make a difference? | we sought to determine the prevalence of infection with chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, mycoplasma genitalium, syphilis, and hiv among african american women who have sex with women (aawsw), and compare sociodemographics, sexual risk behavior characteristics, and sti diagnoses among women reporting sex exclusively with women (exclusive aawsw) to women reporting sex with both women and men (aawswm) during the past 12 months. | 2011 | 22082722 |
| a case of meningitis due to neisseria subflava after ventriculostomy. | surgical site infection (ssi) is an emerging problem in all fields of surgery, as well as in neurosurgery, with numerous strategies against ssi initiated in many countries. in this report, we describe a case of ssi caused by neisseria subflava a month after ventriculostomy placement for acute hydrocephalus. a 53-year old man was referred to our hospital, and was diagnosed with a thalamic hemorrhage with ventricular rupture. bilateral ventriculostomies were performed on the day of presentation. a ... | 2011 | 21894453 |
| neisseria gonorrhoeae with high-level resistance to azithromycin: case report of the first isolate identified in the united states. | we report on the first neisseria gonorrhoeae isolate in the united states identified with high-level resistance to azithromycin. this report discusses the epidemiologic case investigation, the molecular studies of resistance-associated mutations and n. gonorrhoeae multiantigen sequence typing, and challenges posed by emerging gonococcal antimicrobial resistance. | 2011 | 22184617 |
| antimicrobial resistance in neisseria gonorrhoeae in the uk: surveillance and management. | successful antimicrobial therapy is fundamental to the public health control of gonorrhea, in the absence of a protective immune response. neisseria gonorrhoeae, the causative agent, has presented a constant challenge for the provision of such therapy as it has demonstrated the ability to become resistant to successive highly active agents chosen for first-line treatment. acquisition of plasmids from other bacteria and long-term use of a single agent has selected both single step high-level and ... | 2011 | 21973299 |
| crystal structures of two solute receptors for l-cystine and l-cysteine, respectively, of the human pathogen neisseria gonorrhoeae. | atp-binding cassette (abc) transporters are integral membrane proteins that carry a variety of substrates across biological membranes at the expense of atp. the here considered prokaryotic canonical importers consist of three entities: an extracellular solute receptor, two membrane-intrinsic proteins forming a translocation pathway, and two cytoplasmic atp-binding subunits. the ngo0372-74 and ngo2011-14 gene clusters from the human pathogen neisseria gonorrhoeae were predicted by sequence homolo ... | 2011 | 22138345 |
| substrate specificity of low-molecular mass bacterial dd-peptidases. | the bacterial dd-peptidases or penicillin-binding proteins (pbps) catalyze the formation and regulation of cross-links in peptidoglycan biosynthesis. they are classified into two groups, the high-molecular mass (hmm) and low-molecular mass (lmm) enzymes. the latter group, which is subdivided into classes a-c (lmma, -b, and -c, respectively), is believed to catalyze dd-carboxypeptidase and endopeptidase reactions in vivo. to date, the specificity of their reactions with particular elements of pep ... | 2011 | 22029692 |
| phenotypic characterisation of a copa mutant of neisseria gonorrhoeae identifies a link between copper and nitrosative stress. | ngo0579 is annotated as copa in the neisseria gonorrhoeae chromosome, suggesting that it encodes for a cation-transporting atpase specific for copper ions. when compared to wild type cells, a copa mutant was more sensitive to killing by copper ions, but not to other transition metals. the mutant also accumulated a greater amount of copper, which is consistent with the predicted role of copa as a copper efflux pump. the copa mutant showed a reduced ability to invade and survive within human cervi ... | 2011 | 22184419 |
| human heat shock protein (hsp) 90 interferes with neisseria meningitidis adhesin a (nada)-mediated adhesion and invasion. | nada (n eisseria meningitidisadhesin a), a meningococcal surface protein, mediates adhesion to and invasion of human cells, an activity in which host membrane proteins have been implicated. while investigating these host factors in human epithelial cells by affinity chromatography, we discovered an unanticipated interaction of nada with heat shock protein (hsp) 90, a molecular chaperone. the specific in vitro interaction of recombinant soluble nada and hsp90 was confirmed by co-immunoprecipitati ... | 2011 | 22066472 |
| Vaccines of the future. | Vaccines of the future can be divided into three broad groups, namely those of the near future (<10 years); the medium-term future (10-19 years); and the long-term future (20-50 years). For the near future, there is some "low hanging fruit" which is clearly on the horizon, such as a Vi-conjugate vaccine for typhoid or a protein-based vaccine for Neisseria meningitidis serogroup B. Just slightly more distant will be vaccines for shigellosis and a common protein vaccine for Streptococcus pneumonia ... | 2011 | 22185835 |
| Neisseria gonorrhoeae NspA induces specific bactericidal and opsonic antibodies in mice. | Neisseria gonorrhoeae surface protein A (NspA) is a highly conserved gonococcal antigen. To explore the potential of NspA in vaccine development against gonorrhea, BALB/c mice were immunized with pcNspA containing the NspA gene from N. gonorrhoeae strain WHO-A via intramuscular (i.m.) injection, intranasal (i.n.) immunization, or intravaginal (i.vag.) immunization. Following the last DNA immunization, mice were boosted with recombinant NspA (rNspA). Enzyme-linked immunosorbent assays (ELISAs) in ... | 2011 | 21918113 |
| applications of pcr (real-time and masstag) and enzyme-linked immunosorbent assay in diagnosis of respiratory infections and diarrheal illness among deployed u.s. military personnel during exercise balikatan 2009, philippines. | laboratory-based surveillance for diarrheal and respiratory illness was conducted at the 2009 republic of the philippines-united states balikatan exercise to determine the presence of specific pathogens endemic in the locations where the military exercises were conducted. ten stool and 6 respiratory specimens were obtained from individuals meeting case definitions for diarrhea or respiratory illness. stool specimens were frozen in dry ice and remotely tested using enzyme-linked immunosorbent ass ... | 2011 | 22128641 |
| [anti-cytokine activity of microorganisms]. | development of a method of determination of anti-cytokine activity (aca) of microorganisms, study of the prevalence and intensity of aca to pro- and anti-inflammatory cytokines in pathogenic and opportunistic bacteria. | 2011 | 21913393 |
| estimating the prevalence of gonococcal resistance to antimicrobials in england and wales. | gonorrhoea can rapidly develop resistance to antimicrobials and treatment options are becoming depleted. treatment guidelines require robust estimates of the prevalence of resistance but sentinel surveillance systems may not be representative nationally. | 2011 | 21917698 |
| influence of conserved and hypervariable genetic markers on genotyping circulating strains of neisseria gonorrhoeae. | presently there is no vaccine against neisseria gonorrhoeae and therefore accurate information on gonococcal transmission plays a crucial role for interventions designed to limit the spread of infections caused by this microorganism. we evaluated the impact of two different categories of genetic markers, (i) concatenated sequences of 10 housekeeping genes and (ii) hypervariable porb dna sequences, on the genetic relatedness and subsequently on genotyping analysis of this human pathogen. eighty g ... | 2011 | 22163288 |
| increasing trend of resistance to penicillin, tetracycline, and fluoroquinolone resistance in neisseria gonorrhoeae from pakistan (1992-2009). | emergence and spread of drug resistant neisseria gonorrhoeae is global concern. we evaluated trends of antimicrobial resistance in neisseria gonorrhoeae over years 1992-2009 in pakistan. resistance rates were compared between years (2007-2009) and (1992-2006). antimicrobial susceptibility testing was performed and interpreted according to clinical laboratory standards institute (clsi) criteria using the disk diffusion methodology against penicillin, ceftriaxone, tetracycline and ofloxacin. addit ... | 2011 | 21941568 |
| structural bioinformatics of neisseria meningitidis ld-carboxypeptidase: implications for substrate binding and specificity. | neisseria meningitidis, a gram negative bacterium, is the leading cause of bacterial meningitis and severe sepsis. neisseria meningitidis genome contains 2,160 predicted coding regions including 1,000 hypothetical genes. re-annotation of n. meningitidis hypothetical proteins identified nine putative peptidases. among them, the nmb1620 protein was annotated as ld-carboxypeptidase involved in peptidoglycan recycling. structural bioinformatics studies of nmb1620 protein using homology modeling and ... | 2011 | 21983752 |
| response to 'comparative performance of culture using swabs transported in amies medium and the aptima combo 2 nucleic acid amplification test in detection of neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study' | 2011 | 22194583 | |
| penicillin binding proteins as danger signals: meningococcal penicillin binding protein 2 activates dendritic cells through toll-like receptor 4. | neisseria meningitidis is a human pathogen responsible for life-threatening inflammatory diseases. meningococcal penicillin-binding proteins (pbps) and particularly pbp2 are involved in bacterial resistance to β-lactams. here we describe a novel function for pbp2 that activates human and mouse dendritic cells (dc) in a time and dose-dependent manner. pbp2 induces mhc ii (logec50 = 4.7 µg/ml ± 0.1), cd80 (logec50 = 4.88 µg/ml ± 0.15) and cd86 (logec50 = 5.36 µg/ml ± 0.1). this effect was abolishe ... | 2011 | 22046231 |
| first neisseria gonorrhoeae strain with resistance to cefixime causing gonorrhoea treatment failure in austria, 2011. | we describe the first cefixime-resistant neisseria gonorrhoeae strain in austria that caused treatment failure.it follows the first five cases in europe of cefixime treatment failure, reported in norway in 2010 and the united kingdom in 2011. effective treatment of gonorrhoea is crucial for public health control and, at present, requires substantially enhanced awareness, more frequent test-of-cure, interaction with experts after therapeutic failure, tracing and therapy of contacts, and surveilla ... | 2011 | 22085601 |
| interventions to prevent sexually transmitted infections, including hiv infection. | the centers for disease control and prevention (cdc) sexually transmitted disease (std) treatment guidelines were last updated in 2006. to update the "clinical guide to prevention services" section of the 2010 cdc std treatment guidelines, we reviewed the recent science with reference to interventions designed to prevent acquisition of stds, including human immunodeficiency virus (hiv) infection. major interval developments include (1) licensure and uptake of immunization against genital human p ... | 2011 | 22080271 |
| patterns of chlamydia/gonorrhea positivity among voluntarily screened new york city public high school students. | chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) are common sexually transmitted infections that disproportionately affect adolescents. annual screening for ct for sexually active female adolescents is recommended. in 2006, new york city began conducting ct/gc education, screening, and treatment in public high schools. we examine 3-year programmatic outcomes and the relationship between sexual activity, screening, and ct/gc positivity. | 2011 | 21856516 |
| acute bacterial meningitis in infants and children: epidemiology and management. | acute bacterial meningitis (abm) continues to be associated with high mortality and morbidity, despite advances in antimicrobial therapy. the causative organism varies with age, immune function, immunization status, and geographic region, and empiric therapy for meningitis is based on these factors. haemophilus influenzae type b (hib), streptococcus pneumoniae, and neisseria meningitidis cause the majority of cases of abm.disease epidemiology is changing rapidly due to immunization practices and ... | 2011 | 21960054 |
| evaluation of the siemens versant® ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae. | the siemens versant kpcr system is an automated system which combines extraction of nucleic acids from 96 samples with subsequent real-time pcr. the versant ct/gc dna 1.0 (kpcr) assay detects chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) in a multiplex real-time pcr on this system. we compared this assay with the bd probete™ et system (pt) and the roche cobas amplicor (ca). three different sets of samples were tested in the kpcr: pt pre-treated samples, prospectively collected urine ... | 2011 | 21925547 |
| vascular colonization by neisseria meningitidis. | bacterial infection of human vasculature can lead to unregulated systemic activation of coagulation and innate immunity and rapidly becomes life threatening. neisseria meningitidis is a vascular pathogen that causes fatal sepsis and meningitis. post-mortem histological analysis of tissues from individuals infected with n. meningitidis show large bacterial aggregates in close association with the vascular wall of small vessels. the ability of this bacterium to colonize blood vessel endothelium is ... | 2011 | 22185907 |
| pasteurella multocida cmp-sialic acid synthetase and mutants of neisseria meningitidis cmp-sialic acid synthetase with improved substrate promiscuity. | cytidine 5'-monophosphate (cmp)-sialic acid synthetases (csss) catalyze the formation of cmp-sialic acid from ctp and sialic acid, a key step for sialyltransferase-catalyzed biosynthesis of sialic acid-containing oligosaccharides and glycoconjugates. more than 50 different sialic acid forms have been identified in nature. to facilitate the enzymatic synthesis of sialosides with diverse naturally occurring sialic acid forms and their non-natural derivatives, cmp-sialic acid synthetases with promi ... | 2011 | 21968653 |
| mining virulence genes using metagenomics. | when a bacterial genome is compared to the metagenome of an environment it inhabits, most genes recruit at high sequence identity. in free-living bacteria (for instance marine bacteria compared against the ocean metagenome) certain genomic regions are totally absent in recruitment plots, representing therefore genes unique to individual bacterial isolates. we show that these metagenomic islands (mis) are also visible in bacteria living in human hosts when their genomes are compared to sequences ... | 2011 | 22039404 |
| a bacterial prib with weak single-stranded dna binding activity can stimulate the dna unwinding activity of its cognate pria helicase. | bacterial dna replication restart pathways facilitate reinitiation of dna replication following disruptive encounters of a replisome with dna damage, thereby allowing complete and faithful duplication of the genome. in neisseria gonorrhoeae, the primosome proteins that catalyze dna replication restart differ from the well-studied primosome proteins of e. coli with respect to the number of proteins involved and the affinities of their physical interactions: the pria:prib interaction is weak in e. ... | 2011 | 21861872 |
| influence of substrate modification and c-terminal truncation on the active site structure of substrate-bound heme oxygenase from neisseriae meningitidis. a 1h nmr study. | heme oxygenase (ho), from the pathogenic bacterium n. meningitidis(nmho), which secures host iron, shares many properties with mammalian hos but also exhibits some key differences. the crystal structure appears more compact, and the crystal-undetected c-terminus interacts with substrate in solution. the unique nature of substrate-protein, specifically pyrrole-i/ii-helix-2, peripheral interactions in nmho are probed by 2d (1)h nmr to reveal unique structural features controlling substrate orienta ... | 2011 | 21870860 |
| the epidemiology of human papillomavirus infection in hiv-positive and hiv-negative high-risk women in kigali, rwanda. | abstract: | 2011 | 22136570 |
| phenotypic and molecular characterization of serogroup c neisseria meningitidis associated with an outbreak in bahia, brazil. | objective: to characterize meningococcal strains isolated from five cases of meningococcal disease (md) associated with an outbreak in trancoso - ba, occurred in october 2009. all cases, with the exception of a 39-year-old male, attended a dance party with approximately 1000 youngsters in a rural site. materials and methods: the epidemiological investigation was conducted by the epidemiological surveillance service of bahia state. meningococcal strains were characterized at adolfo lutz institute ... | 2011 | 22078548 |
| characterization of profile of multidrug-resistant neisseria gonorrhoeae using old and new definitions in india over a decade: 2000-2009. | the burden of "multidrug-resistant neisseria gonorrhoeae" (mdr-ng) was high considering the old definition (26.0%). according to the new definitions, no strain was mdr or extensively drug-resistant. the emergence of resistance to ceftriaxone and cefixime will lead to detection of mdr-ng and may be extensively drug-resistant ng, even according to the new definition. | 2011 | 21992984 |
| characterization of a large genomic deletion in four irish families with c7 deficiency. | inherited deficiency of the seventh complement component (c7) is associated with increased susceptibility to neisseria meningitidis infections. the disease is rare in most western countries. here we report new investigations of a large, but incompletely characterized genomic deletion of exons 8 and 9 [c.739-?_1093+?del], previously identified in three unrelated irish families with c7 deficiency. we have analysed dna from one individual, who is homozygous for the deletion, by pcr using primers pr ... | 2011 | 22206826 |
| carriage of neisseria meningitidis in europe: a review of studies undertaken in the region. | meningococcal conjugate vaccines induce herd protection by preventing nasopharyngeal meningococcal acquisition, which is a prerequisite for invasive disease. thus, meningococcal carriage epidemiology is important in understanding relationships between carriage and disease. a literature search traced information on meningococcal carriage in 27 eu countries. meningococcal carriage prevalence differed within and between countries, varying across age groups, serogroup distribution and over time. car ... | 2011 | 21905785 |
| implementation and impact of a meningococcal c conjugate vaccination program in 13- to 25-year-old individuals in galicia, spain. | background: in response to increased case numbers of meningococcal group c disease, catch-up vaccination strategies have been shown to be successful. this paper describes the results of a repeat vaccination program in galicia, spain, and the strategy used for it. methods and results: three vaccination waves were performed: first, in 1996/1997 with a meningococcal group a and c polysaccharide vaccine in individuals aged 18 months to 19 years; second, in 2000 with a conjugate serogroup c polysacc ... | 2011 | 21957332 |
| effects of multi-walled carbon nanotubes (mwcnt) under neisseria meningitidis transformation process. | this study aimed at verifying the action of multi-walled carbon nanotubes (mwcnt) under the naturally transformable neisseria meningitidis against two different dna obtained from isogenic mutants of this microorganism, an important pathogen implicated in the genetic horizontal transfer of dna, causing the escape of the principal vaccination measured worldwide by the capsular switching process. | 2011 | 22088149 |
| identification of a conserved protein involved in anaerobic unsaturated fatty acid synthesis in neiserria gonorrhoeae: implications for facultative and obligate anaerobes that lack faba. | transcriptome analysis of the facultative anaerobe, neisseria gonorrhoeae, revealed that many genes of unknown function were induced under anaerobic conditions. mutation of one such gene, ngo1024, encoding a protein belonging to the 2-nitropropane dioxygenase-like superfamily of proteins, was found to result in an inability of gonococci to grow anaerobically. anaerobic growth of an ng1024 mutant was restored upon supplementation with unsaturated fatty acids (ufa), but not with the saturated fatt ... | 2011 | 21895795 |
| the prevalence of chlamydia trachomatis and neisseria gonorrhoeae infections among men with urethritis in kuwait. | chlamydial non-gonococcal urethritis and gonorrhoea are the most common sexually transmitted bacterial infections worldwide. data on these infections are scanty in the islamic world, especially kuwait. the objective of this study was to establish the prevalence of chlamydia trachomatis and neisseria gonorrhoeae among men with symptomatic urethritis in kuwait. | 2011 | 22000844 |
| hiv/std pattern and its associated risk factors among male std clinic attendees in china: a foci for hiv intervention. | abstract: | 2011 | 22200257 |
| a novel mechanism of high-level, broad-spectrum antibiotic resistance caused by a single base pair change in neisseria gonorrhoeae. | the mtrc-mtrd-mtre multidrug efflux pump of neisseria gonorrhoeae confers resistance to a diverse array of antimicrobial agents by transporting these toxic compounds out of the gonococcus. frequently in gonococcal strains, the expression of the mtrcde operon is differentially regulated by both a repressor, mtrr, and an activator, mtra. the mtrr gene lies 250 bp upstream of and is transcribed divergently from the mtrcde operon. previous research has shown that mutations in the mtrr coding region ... | 2011 | 21933917 |
| antibiotics for preventing meningococcal infections. | meningococcal disease is a contagious bacterial infection caused by neisseria meningitidis (n. meningitidis). household contacts have the highest risk of contracting the disease during the first week of a case being detected. prophylaxis is considered for close contacts of people with a meningococcal infection and populations with known high carriage rates. | 2011 | 21833949 |
| general metabolism of laribacter hongkongensis: a genome-wide analysis. | 2011 | 21711917 | |
| Deletion of one nucleotide within the homonucleotide tract present in the hsdS gene alters the DNA sequence specificity of type I restriction-modification system NgoAV. | As a result of a frameshift mutation, the hsdS locus of the NgoAV type IC restriction and modification (RM) system comprises two genes, hsdS(NgoAV1) and hsdS(NgoAV2). The specificity subunit, HsdS(NgoAV), the product of the hsdS(NgoAV1) gene, is a naturally truncated form of an archetypal specificity subunit (208 N-terminal amino acids instead of 410). The presence of a homonucleotide tract of seven guanines (poly[G]) at the 3' end of the hsdS(NgoAV1) gene makes the NgoAV system a strong candida ... | 2011 | 21984785 |
| testing men who have sex with men for urethral infection with chlamydia trachomatis and neisseria gonorrhoeae is only half the job, and we need the right tools. | 2011 | 21934566 | |
| emergence of multidrug-resistant neisseria gonorrhoeae strains circulating worldwide. | 2011 | 22168311 | |
| Age-related immunity to meningococcal serogroup C vaccination: an increase in the persistence of IgG2 correlates with a decrease in the avidity of IgG. | All children and adolescents between 1 and 19 years of age in The Netherlands received a single meningococcal serogroup C conjugate (MenCC) vaccine in 2002. During follow-up 4-5 years later, the persistence of MenC polysaccharide-specific IgG was found to be dependent on age of vaccination with higher IgG levels in the oldest immunized age categories. | 2011 | 21887261 |
| three-dimensional obstacles for bacterial surface motility. | twitching motility enables bacteria to move over surfaces using type iv pili as grappling hooks. here it is shown that the motility of the round neisseria gonorrhoeae as well as of rod-shaped myxococcus xanthus is guided by elevations with dimension and depth corresponding to the size of the bacteria. | 2011 | 22183854 |
| chlamydia trachomatis prevalence and risk behaviors in parturient women aged 15 to 24 in brazil. | chlamydia trachomatis (ct) is a sexually transmitted infection having repercussions on reproductive health and impact on the foetus. our goal was to estimate the prevalence of and risk factors for ct in young parturient women in brazil. | 2011 | 21934572 |
| Universal protein vaccines against Neisseria meningitidis serogroup B, Streptococcus pneumoniae and influenza. | Traditional vaccines can only be really effective when the antigenic diversity or variability of the micro-organisms in the same bacterial species or viral family is no more than marginally different, and when protection is mainly dependent from antibody-mediated immunity. One solution is to identify the antigens common to all of the strains in a species or family that are significantly capable of eliciting inactivating antibodies. A number of attempts have recently been made to develop vaccines ... | 2011 | 21865879 |
| comparative long-term adverse effects elicited by invasive group b and c meningococcal infections. | given the identity between neisseria meningitidis serogroup b (menb) capsular polysaccharide (polysialic acid; psa) and psa found on neural cell adhesion molecules, it has been proposed that infection with menb or vaccination with psa may be associated with subsequent autoimmune or neurological disease. | 2011 | 21946191 |
| Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. | Please cite this paper as: Allard et al. (2011) Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00297.x. Background Shared seasonal patterns, such as between influenza and some respiratory bacterial infections, can create associations between phenomena not causally related. Objectives To estimate the association of influenza with subsequent bacterial infections after ... | 2011 | 21985083 |
| clinical validation of multiplex real-time pcr assays for detection of bacterial meningitis pathogens. | neisseria meningitidis (nm), haemophilus influenzae (hi), and streptococcus pneumoniae (sp) are important causes of meningitis and other infections, and rapid, sensitive, and specific laboratory assays are critical for effective public health interventions. singleplex real-time pcr assays have been developed to detect nm ctra, hi hpd and sp lyta, and serogroup-specific genes in the cap locus for nm serogroups a, b, c, w135, x and y. however, the assay sensitivity for serogroups b, w135 and y is ... | 2011 | 22170919 |