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phenotypic and genotypic diversity of streptococcus sanguis in infants.streptococcus sanguis comprises a heterogeneous group of oral streptococci indigenous to the oral cavity of humans. a total of 289 isolates from an infant population (n=37) were tentatively identified as s. sanguis on the basis of the distinctive colony morphology as shown on mm10-sucrose non-selective medium. these isolates were divided into four biovars of s. sanguis as determined by an extended panel of biochemical attributes. chromosomal dna was extracted from each isolate, and an ap-pcr fin ...200111442849
endocarditis caused by penicillin-resistant viridans streptococci: 2 cases and controversies in therapy.although penicillin-resistant viridans streptococci have been isolated from samples from the mouth, blood, and wounds in increasing numbers, viridans streptococci isolated from patients with endocarditis have remained sensitive to penicillin for the past 5 decades. we report the cases of 2 patients with penicillin-resistant viridans streptococcal endocarditis, review 6 other cases from the literature, and summarize 2 studies that used an animal model of penicillin-resistant viridans streptococca ...200111462199
do viridans streptococci cause pneumonia in children? 200111465856
enterococcus faecalis--a mechanism for its role in endodontic failure.the aim of this study was to identify a possible mechanism that would explain how e. faecalis could survive and grow within dentinal tubules and reinfect an obturated root canal.200111482724
mannosidase production by viridans group streptococci.the production of mannosidase activity by all currently recognized species of human viridans group streptococci was determined using an assay in which bacterial growth was dependent on the degradation of the high-mannose-type glycans of rnase b and subsequent utilization of released mannose. rnase b is an excellent substrate for the demonstration of mannosidase activity since it is a glycoprotein with a single glycosylation site which is occupied by high-mannose-type glycoforms containing five t ...200111230417
binding of oral streptococci to human fibrinogen.the interaction of oral streptococci with human fibrinogen was investigated. streptococcus gordonii was chosen as a representative species to study the binding to fibrinogen. s. gordonii dl1 adhered to immobilized fibrinogen and bovine serum albumin. binding to immobilized fibrinogen was saturable, concentration and temperature dependent. the binding of s. gordonii dl1 to fibrinogen was inhibited by anti-fibrinogen antibody. heating of the bacteria for 1 h at 95 degrees c resulted in 90% inhibit ...200111240861
ph-regulated secretion of a glyceraldehyde-3-phosphate dehydrogenase from streptococcus gordonii fss2: purification, characterization, and cloning of the gene encoding this enzyme.streptococcus gordonii and other viridans streptococci (vs) are primary etiologic agents of infective endocarditis, despite being part of the normal oral microflora. recently, a surface-bound glyceraldehyde-3-phosphate dehydrogenase (gapdh) has been found on the cells of all tested streptococcal species, where it has been implicated as a virulence factor. in contrast, we observed that a soluble extracellular gapdh was the major secreted protein from s. gordonii fss2, an endocarditis strain. the ...200111269731
oral colonization and cariogenicity of streptococcus gordonii in specific pathogen-free tan:spfom(om)br rats consuming starch or sucrose diets.the significance of streptococcus gordonii in dental caries is undefined, as is that of other alpha-amylase-binding bacteria (abb) commonly found in the mouth. to clarify the ecological and cariological roles of s. gordonii our specific pathogen-free osborne-mendel rats, tan:spfom(om)br, were fed either diet 2000 (containing 56% confectioner's sugar, most of which is sucrose) or diet 2000cs (containing 56% cornstarch, in lieu of confectioner's sugar) and inoculated with s. gordonii strains. unin ...200111269866
monocytes augment bacterial species- and strain-dependent induction of tissue factor activity in bacterium-infected human vascular endothelial cells.in bacterial endocarditis (be), intravascular infection with staphylococcus aureus, streptococcus sanguis, or staphylococcus epidermidis can lead to formation of a fibrin clot on the inner surface of the heart and cause heart dysfunction. the events that start the coagulation in the early stage of the disease are largely unknown. we have recently shown that human endothelial cells (ec) upon binding and internalization of s. aureus, but not s. sanguis or s. epidermidis, express tissue factor (tf) ...200111292691
interleukin-10 regulates the tissue factor activity of monocytes in an in vitro model of bacterial endocarditis.monocytes are important effector cells in the pathogenesis of bacterial endocarditis since they provide the tissue factor that activates the coagulation system and maintains established vegetations. monocytes secrete cytokines that can modulate monocyte tissue factor activity (tfa), thereby affecting the formation and maintenance of vegetations. in this study, we show that monocytes cultured for 4 h on a streptococcus sanguis-infected fibrin matrix mimicking the in vivo vegetational surface expr ...200111292741
utilization and acid production of beta-galactosyllactose by oral streptococci and human dental plaque.beta-galactosyllactose is a trisaccharide containing the beta-galactosidic linkage at the nonreducing end. the purpose of this study was to determine whether certain oral streptococci could utilize four kinds of beta-galactosyllactoses. three of four beta-galactosyllactoses were unable to support growth of the oral streptococci and to be a substrate for producing acid from the cell suspensions and dental plaque. 4'-beta-galactosyllactose supported growth of streptococcus sanguis atcc 35105, atcc ...200111169141
properties of streptococcus sanguinis glucans formed under various conditions.the aim of our study was to determine whether the structure of glucans formed by glucosyltransferase from streptococcus sanguinis (gtfss) on a surface differ from those formed in solution and to explore the effects of antiserum to gtfs, control normal rabbit serum, starch hydrolysates (sth) and dextran on s. sanguinis (gtfss) glucan. linkage analyses showed that solution-formed glucans are predominantly alpha-1,6-linked and have a small amount of alpha-1,3-linked glucose. surface-formed glucans ...200111125200
bacterial detachment from salivary conditioning films by dentifrice supernates.this study compared the detachment by supernates of nine different dentifrices of four oral bacterial strains adhering to a salivary pellicle in a parallel plate flow chamber. ultra-thin bovine enamel slabs were coated for 1.5 h with human whole saliva. following buffer rinsing, a bacterial suspension of streptococcus oralis, streptococcus sanguis, streptococcus mutans or actinomyces naeslundii was perfused through the flow chamber at a shear rate of 30 s-1 for four hours, and the number of adhe ...200211507932
vitality status of microorganisms in infected human root dentine.this experimental study was initiated to establish a method for characterizing the vitality status of bacteria in infected human root dentine by differentiating between viable and dead microorganisms.200211843972
a protocol for polymerase chain reaction detection of enterococcus faecalis and enterococcus faecium from the root canal.the present study was set up to develop a protocol for detection of enterococcus faecalis and enterococcus faecium from the root canal.200211853234
streptococcus sanguis secretes cd14-binding proteins that stimulate cytokine synthesis: a clue to the pathogenesis of infective (bacterial) endocarditis?streptococcus sanguis is the major causative organism of infective (bacterial) endocarditis but, surprisingly, almost nothing is known about how it induces endocardial inflammation. in earlier studies we have shown that many bacteria secrete potent cytokine-inducing or -inhibiting proteins. we have therefore isolated the material secreted by s. sanguis grown on blood agar or in broth culture and have tested its ability to induce human peripheral blood monocytes to synthesize pro-inflammatory cyt ...200211855941
initial characterization of the streptococcus gordonii htpx gene.examination of the streptococcus gordonii chromosomal region, which lies immediately upstream of the glucosyltransferase positive regulatory determinant rgg, revealed two open reading frames. based on nucleotide sequences, these genes were similar to the listeria monocytogenes lema gene, which is involved in antigen presentation, and the escherichia coli htpx heat shock gene, which has an unknown function. northern hybridization analysis indicated that s. gordonii lema and htpx genes were associ ...200211860552
the effect of tissue molecules on bacterial invasion of dentine.bacterial invasion of dentinal tubules is a critical step in the pathogenesis of dental caries and pulp and periapical disease. the purpose of this study was to determine the effect some molecules commonly found in saliva and dentinal tubule fluid may have on the bacterial invasion of dentine. the results showed that invasion of streptococcus gordonii or enterococcus faecalis cells was inhibited when the bacterial cells were in solution with mucin, immunoglobulin g (igg) and serum, and this was ...200211860553
streptococcus sinensis sp. nov., a novel species isolated from a patient with infective endocarditis.a bacterium was isolated from the blood culture of a patient with infective endocarditis. the cells were facultative anaerobic, nonsporulating, gram-positive cocci arranged in chains. the bacterium grows on sheep blood agar as alpha-hemolytic, gray colonies of 0.5 to 1 mm in diameter after 24 h of incubation at 37 degrees c in ambient air. growth also occurs in 10 or 40% bile and on bile esculin agar but not in 6% nacl. no enhancement of growth is observed in 5% co(2). it is nongroupable with la ...200211880397
subgingival microbiota of indigenous indians of central america.to define the subgingival microbial profiles of adult subjects from a previously identified rural community of indigenous indians in guatemala, central america.200211895544
fluoride and organic weak acids as respiration inhibitors for oral streptococci in acidified environments.oxygen metabolism (respiration) of streptococcus mutans gs-5 involving nadh oxidases, mainly of the h(2)o-producing type, was found to be acid sensitive, as was nadh oxidase activity of cell extracts. respiration of intact cells in acidified media was also highly sensitive to fluoride, with a 50% inhibitory concentration of about 0.02 mm at ph 4. in contrast, nadh oxidases in cell extracts were fluoride insensitive. fluoride inhibition of respiration of intact cells was related to weak-acid effe ...200211929560
[therapeutic impact of streptococcal and enterococcal bacteremia in hematology patients].from january 1999 to may 2000 (17 months), 21 strains of streptococci and four strains of enterococci have been isolated from 74 blood cultures in 25 infectious episodes in hematologic patients. they concerned 21 patients, of 21 to 77 years old. these patients suffered from acute leukaemia (14 cases), chronic lymphoid leukaemia (two cases), non-hodgkin's lymphoma (two cases) or myeloma (three cases). seventeen patients displayed a single streptococcal or enterococcal episode, two had two episode ...200211980330
asymmetric primary ringless repair of mitral valve endocarditis: a case report.the case is presented of a patient with a large mitral valve vegetation. a technique of mitral valve repair involving ringless annuloplasty repair following bileaflet resection of the infected regions of the valve is described. no pledgets or prosthetic material were used in the repair. the patient recovered uneventfully, with trivial mitral regurgitation documented on post-repair echocardiography. this technique may suggest a method for early intervention in mitral endocarditis.200212000175
particulate bioglass reduces the viability of bacterial biofilms formed on its surface in an in vitro model.45s5 bioglass is a bioactive implant material which, in its particulate form, is used in the repair of periodontal defects. the surface reactions undergone by this material in an aqueous environment may exert an antibacterial effect that would be beneficial to periodontal surgical treatment. biofilms of streptococcus sanguis, an early plaque former, and mixed species biofilms from a salivary inoculum grown under conditions similar to those associated with periodontal implants, were grown on part ...200212005145
simultaneous measurement of the viability, aggregation, and live and dead adherence of streptococcus crista, streptococcus mutans and actinobacillus actinomycetemcomitans in human saliva in relation to indices of caries, dental plaque and periodontal disease.salivary proteins have multiple functions and many share similar functions, which may be why it has been difficult to relate variations in their concentrations to oral health and ecology. an alternative is to focus on variations in the major functions of saliva. an hydroxyapatite-coated microplate model has been developed that simultaneously measures saliva-promoted bacterial viability, bacterial aggregation, and live and dead bacterial adherence, while simulating oral temperature and shearing f ...200212015215
effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms.antimicrobial effects of fluoride in vivo remain contentious. previous studies suggested that 1 mm naf reduced acid production from glucose, and prevented the enrichment of bacteria associated with caries in a chemostat model. the present study examines the effects of a lower fluoride concentration (0.53 mm, 10 ppm naf) in both biofilm and planktonic microbial communities. nine oral species were grown at ph 7.0 and pulsed on 10 successive days with glucose; bacterial metabolism was allowed to re ...200212037363
[clinical and laboratory studies of bacterial adhesion to validate the choice of material for making provisional dentures for patients with periodontal diseases].adhesion of bacteria favoring the development of oral inflammations, including cariesogenic and periodontopathogenic (actinobacillus actinomycetemcommitans, streptococcus sanguis, fusobacterium nucleatum, staphylococcus warneri) and yeast fungi (candida albicans), to 13 materials used for making provisional dentures was studied. adhesion of all the studied bacteria and fungi to russian material esterfil foto was the minimum. clinical use of this material in patients with chronic generalized peri ...200212056141
mitral valve aneurysm associated with aortic valve endocarditis and regurgitation.mitral valve aneurysms are rare complications occurring most commonly in association with aortic valve infective endocarditis. [decroly 1989, chua 1990, northridge 1991, karalis 1992, roguin 1996, mollod 1997, vilacosta 1997, cai 1999, vilacosta 1999, teskey 1999, chan 2000, goh 2000, marcos- alberca 2000] while the mechanism of the development of this lesion is unclear, complications such as perforation can occur and lead to significant mitral regurgitation. [decroly 1989, karalis 1992, teskey ...200212538147
groesl sequence determination, phylogenetic analysis, and species differentiation for viridans group streptococci.the full-length sequences of the groesl genes (also known as cpn10/60) of streptococcus anginosus, streptococcus constellatus, streptococcus gordonii, and streptococcus sanguis and the near full-length sequence of the groesl genes of streptococcus intermedius, streptococcus bovis, streptococcus mitis, streptococcus mutans, streptococcus oralis, and streptococcus salivarius were determined. the lengths of the groes genes from the 10 species listed above ranged from 282 to 288 bp, and the full-len ...200212202549
enhanced neutrophil emigration and porphyromonas gingivalis reduction following pgg-glucan treatment of mice.periodontal disease is the consequence of a mixed gram-negative infection in the gingival sulcus and has been associated with deficits in the neutrophil response. a novel, and heretofore untested, alternative approach to therapy is the use of biological-response modulators that enhance the neutrophil response. poly-beta1-6-glucotriosyl-beta1-3-glucopyranose glucan (pgg-glucan) is an immunomodulator, derived from yeast, which specifically enhances neutrophil priming, phagocytosis and bacterial ki ...200212221019
[microbiological validation of the choice of basic plastic for removable dentures].adhesion of obligate and facultative anaerobic bacteria, favoring the development of oral inflammatory diseases, including the cariesogenic and periodontogenic bacteria and candida albicans fungi, isolated from patients with periodontitis, to 13 basic materials used in removable denture making, was studied. the adhesion of all bacteria (streptococcus sanguis, prevotella melangogenica, fusobacterium nucleatum, corynebacterium xerosis) and fungi to hot polymerization basic materials was the maximu ...200212224327
in vitro study of transmission of bacteria from contaminated metal models to stone models via impressions.the purpose of this study was to determine the risk of microbiological transmission into the dental laboratory via impressions and dental stone models. metal master models were contaminated with bacillus subtilis and streptococcus sanguis. impressions in alginate, polyvinylsiloxane, and polyether were used, and models were cast in two brands of dental stone. samples were taken from the impression surfaces before and after casting, and sections from the stone models were taken by a technique deve ...200212271350
streptococcus sanguis meningitis: report of a case.a 35-year-old woman who presented with acute purulent meningitis and hearing loss was reported. no bacteria was seen with gram' s stain of cerebrospinal fluid (csf). csf grew streptococcus viridans, which was subsequently identified to be s. sanguis. the patient improved after treatment, including hearing.200212296418
monocytes maintain tissue factor activity after cytolysis of bacteria-infected endothelial cells in an in vitro model of bacterial endocarditis.intravascular infection with staphylococcus aureus, staphylococcus epidermidis, or streptococcus sanguis can initiate fibrin formation on endocardial tissue, causing bacterial endocarditis. the ability of these bacteria to injure intact endothelial cells (ecs) and to aggravate tissue factor (tf)-dependent coagulation in the presence of blood leukocytes was investigated. cytolysis of ecs occurred after infection with s. aureus and, with membrane-bound monocytes or granulocytes present, also after ...200212355366
the effect of para-aminobenzoic acid on growth and metabolism of porphyromonas gingivalis.to study the effect of para-aminobenzoic acid (paba) on the growth and metabolism of porphyromonas gingivalis (p.g).200212411175
the relativity between streptococcus sanguis group and coronary heart disease.to study the relativity between streptococcus sanguis group (ssg) and coronary heart disease (chd).200212419151
a retrospective investigation of advanced periodontal disease as a risk factor for septicemia in hematopoietic stem cell and bone marrow transplant recipients.septicemia is a cause of death in hematopoietic stem cell transplant (hsct) recipients. extraction of teeth with advanced periodontitis has been advocated before hsct to prevent septicemia in myeloablated hosts. the primary aim of the present study was to determine impact of chronic periodontitis, as measured by radiographic alveolar bone loss, on septicemia and transplant mortality.200212424452
increased proliferative response of peripheral blood mononuclear cells and t cells to streptococcus mutans and glucosyltransferase d antigens in the exacerbation stage of recurrent aphthous ulcerations.cytotoxic t lymphocyte-induced lysis of virus-infected oral epithelial cells has been shown to be a cause of early ulcer formation in recurrent aphthous ulcerations (rau). to test whether bacteria and their associated antigens are involved in the disease process of rau, the proliferative response (pr) to different streptococcal species in peripheral blood mononuclear cells (pbmc) and t cells isolated from rau patients at the exacerbation stage was determined.200212440086
selective lethal photosensitization of methicillin-resistant staphylococcus aureus using an igg-tin (iv) chlorin e6 conjugate.the growing resistance of methicillin-resistant staphylococcus aureus (mrsa) to conventional antimicrobial agents necessitates the development of alternative approaches to preventing and treating infections. one such approach is photodynamic therapy, whereby target cells are treated with light-activated drugs (photosensitizers). this investigation aimed to determine whether the ability of mrsa to express the igg-binding protein, protein a, could be exploited to enable selective lethal photosensi ...200212461004
bactericidal effect of the er:yag laser on dental implant surfaces: an in vitro study.the aim of the in vitro study was to examine the bactericidal effect of an er:yag laser on common dental implant surfaces.200212479633
correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese.the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ...200212483235
henoch-schonlein purpura secondary to subacute bacterial endocarditis.henoch-schönlein purpura (hsp), a systemic, small-vessel vasculitic syndrome, is characterized by a nonthrombocytopenic purpuric rash, arthralgia, abdominal pain, and nephritis. these signs and symptoms may occur in any order, and not all are necessary for the diagnosis. although most common in 4- to 7-year-olds, hsp is well documented in adults and is often preceded by a history of mucosal-based infections, especially of the upper respiratory tract. we report a case of hsp that occurred coincid ...200212080945
a role for glycoprotein ib in streptococcus sanguis-induced platelet aggregation.numerous studies have implicated bacteria in cardiovascular disease, but there is a paucity of information on the mechanism involved. in this study we show how the common oral bacterium streptococcus sanguis can directly interact with platelets, resulting in activation and aggregate formation. platelet aggregation was dependent on glycoprotein iib/iiia (gpiib/iiia) and thromboxane. platelets could also directly bind to s sanguis, but this interaction was not inhibited by gpiib/iiia antagonists. ...200212091342
roles of salivary proteins in the adherence of oral streptococci to various orthodontic brackets.knowledge of salivary pellicles on orthodontic brackets provides a better understanding of microbial adherence. the aim of this study was to analyze the effects of bracket pellicles on the adherence of streptococcus gordonii and streptococcus mutans. bracket pellicles were formed by the incubation of 4 kinds of orthodontic brackets with unstimulated whole saliva for 2 hrs, and analyzed by electrophoresis, immunodetection, and amino acid analysis. binding assays were then performed by the incubat ...200212097434
septic arthritis due to streptococcus sanguis.we report an unusual case of septic arthritis due to streptococcus sanguis, a member of the viridans group of streptococci that are part of the normal flora of the mouth and upper respiratory tract. our patient had severe underlying periodontal disease, which likely contributed to his joint sepsis through hematogenous spread. although viridans streptococci are rare causes of septic arthritis in native joints, they should be considered in the setting of severe periodontal disease.200212108609
influence of secondary colonizers and human plasma on the adherence of porphyromonas gingivalis in vitro.the influence of secondary colonizers (fusobacterium nucleatum and actinomyces naeslundii) and the effect of human plasma on the adherence of porphyromonas gingivalis were investigated. hydroxyapatite (hap) discs coated with streptococcus sanguis were immersed in a 3h-labeled bacterial cell suspension of f. nucleatum or a. naeslundii and then in a 14c-labeled p. gingivalis cell suspension. bacterial cells on the discs were pyrolysed to quantify the radioisotopes released. the cell numbers of sec ...200212120708
intracellular and extracellular phs of streptococcus mutans after addition of acids: loading and efflux of a fluorescent ph indicator in streptococcal cells.a ph-sensitive fluorescent dye, 2', 7'-bis-(2-carboxyethyl)-5 and 6-carboxyfluorescein (bcecf), was used to determine intracellular ph (ph(in)). the efflux of bcecf loaded into oral streptococcal cells was determined after incubation of the cells at 35 degrees c for 20 min in the presence and absence of glucose. in the absence of glucose, the fluorescence of intracellular bcecf in streptococcus mutans, streptococcus sanguis, streptococcus salivarius and streptococcus sobrinus decreased only very ...200212121474
the relationship between odontogenic bacteraemia and orthodontic treatment procedures.the purpose of this research was to estimate the prevalence and intensity of bacteraemia associated with orthodontic treatment procedures. the four procedures investigated were: an upper alginate impression, separator placement, band placement, and adjustment of an archwire on a fixed appliance. eighty-one children undergoing general anaesthesia (ga) for dento-alveolar surgery related to their orthodontic treatment were randomly allocated to the impression or separator group. a further 61 childr ...200212143093
streptococcus sanguis modulates type ii collagen-induced arthritis in dba/1j mice.native type ii collagen is tolerogenic when given orally or i.p. to dba/1j mice and induces autoimmune arthritis when given s.c. in cfa. the tolerogenic epitope is contained in cyanogen bromide fragment 11 (cb11) and is structurally mimicked by pgeqgpk within the platelet aggregation-associated protein (paap) on streptococcus sanguis. to learn whether s. sanguis modulates transmucosally the ag-specific development of autoimmune arthritis, dba/1j pups were given live s. sanguis, cb11, or type ii ...200212165549
in vitro testing of xylitol as an anticariogenic agent.a number of studies involving xylitol chewing gum have demonstrated that xylitol is both noncariogenic and anticariogenic. the ability of xylitol to act as an anticariogenic agent most likely is due to its ability to be transported into caries-causing oral bacteria and inhibiting fermentation either by depleting the cell of high-energy phosphate or by poisoning the glycolytic system. in vitro tests were conducted to determine the concentration of xylitol required to inhibit the growth of three s ...200212640850
[the influence of two implant materials on the growth of three subgingival predominant bacteria].commercially pure titanium and titanium alloy as dental implants have show impressive clinical results. despite the high success rates, some implants do fail. compared to those studies on osseointegration of implants, the information pertaining to their failure is little. further studies on the relationship between implant material and micro-organism are needed. the purpose of this investigation is to study the effect of two commonly used implant materials titanium (ta2) and ti-6ai-4v alloy (tc4 ...200212599430
[influence of different dna extractions on the identification of streptococcus sanguis group by arbitrary primed polymerase chain reaction]objective: to assess the influence of different dna extractions on the identification of streptococcus sanguis group (ssg) species by arbitrary primed polymerase chain reaction (ap-pcr). methods: ap-pcr was used to distinguish ssg species by designing 25bp arbitrary primer 5'aag aga gga gct agc tct tct tgg a 3', and the genomic dna was extracted by 3 methods. results: there were great differences in the main band of dna polymorphism among ssg species. the similar band could be got from the diffe ...200212601867
[the role of dead bacteria in streptococcus sanguis biofilm reformation].to investigate the role of dead bacteria in streptococcus sanguis biofilm reformation.200214983332
[the in vitro study on polistes mandarinus' effects on cariogenic bacteria].to study the role of polistes mandarinus on the growth,acid generation and water insoluble glucan(wig) production of streptococcus mutans, streptococcus sanguis and actinomyces viscosus as a preparation for screening an effective agent ot medicate the balance of oral microflora.200214983338
efficacy of naocl/h2o2 irrigation and gaalas laser in decontamination of root canals in vitro.to investigate the bactericidal effect of an 809 nm semiconductor laser alone, and in combination with naocl/h(2)o(2) irrigation in root canals in vitro.200312605425
a rapid solid-phase fluorimetric assay for measuring bacterial adherence, using dna-binding stains.in this report, we describe the validation of a rapid, single-step, microtiter plate method for quantifying bacterial adherence, based on fluorescent labeling of microorganisms with cell-permeable fluorescent dna-binding probes. we have tested the binding to saliva-coated microtiter plates of bacteria, including helicobacter pylori and viridans streptococci (s. mitis, s. gordonii, s. sanguis), known to interact with salivary components. furthermore, we tested the short-term and longer-term tempo ...200312609723
bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy.this study was an attempt to clarify whether the bactericidal effects of photodynamic therapy (pdt) are wavelength or dose-dependent. we also attempted to create an optimised protocol for a light-based bactericidal modality to eliminate periodontal pathogens. cultures of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphromonas gingivalis, prevotella intermedia, and streptococcus sanguis, were exposed to a he-ne laser (632.8 nm) with a 30 mw power output, a 100 mw diode laser a ...200312627274
the diagnosis and management of recurrent aphthous stomatitis: a consensus approach.recurrent aphthous stomatitis, or ras, is a common oral disorder of uncertain etiopathogenesis for which symptomatic therapy only is available. this article reviews the current data on the etiopathogenesis, diagnosis and management of ras in a primary care setting.200312636124
the anti-adherence effect of piper betle and psidium guajava extracts on the adhesion of early settlers in dental plaque to saliva-coated glass surfaces.the aqueous extracts of piper betle and psidium guajava were prepared and tested for their anti-adherence effect on the adhesion of early plaque settlers (strep. mitis, strep. sanguinis and actinomyces sp.). the saliva-coated glass surfaces were used to simulate the pellicle-coated enamel surface in the oral cavity. our results showed that the anti-adherence activities of piper betle and psidium guajava extracts towards the bacteria were different between the bacterial species. psidium guajava w ...200314763515
identification of streptococcus sanguinis with a pcr-generated species-specific dna probe.the objective of the present study was to design a pcr-generated dna probe and determine the specificity of the probe for the identification of clinical isolates of streptococcus sanguinis. to do this, we examined over 200 arbitrarily primed pcr (ap-pcr) amplicon patterns obtained with dna from clinical isolates of s. sanguinis. a 1.6-kb dna amplicon that was common to all ap-pcr profiles was extracted from agarose gels and then cloned and sequenced. a search for a similar sequence in the genban ...200312904342
immunopathogenesis and prevention of uveitis with the behçet's disease-specific peptide linked to cholera toxin b. 200312918685
the role of infectious agents in the pathogenesis of behçet's disease. 200312918686
detection of microbial dna in skin lesions from patients with behçet's disease. 200312918687
antimicrobial activity of synthetic human cap18 peptides to streptococcus sanguis isolated from patients with behçet's disease. 200312918689
cytokine production of peripheral blood mononuclear cells stimulated with streptococcus sanguis antigen in patients with behçet's disease. 200312918702
the antimicrobial efficacy of 'mgp' gutta-percha in vitro.to determine whether 'mgp' gutta-percha (westport, ct, usa), a commercially available gutta-percha containing iodoform, inhibits the growth of potential endodontic pathogens.200312950576
comparative antistreptococcal activity of photobactericidal agents.in order to establish a comparative order of efficacy among established photosensitising compounds currently under investigation, the in vitro photobactericidal activities of six commercially available photosensitisers were investigated at equal concentration against streptococcus sanguis using a helium neon (hene) laser (632.8 nm). of the photosensitisers used, the four phenothiazinium compounds were efficient photobactericidal agents as was the protoporphyrin ix salt. however, the zinc phthalo ...200312962360
comparison of antiadhesive and antibacterial effects of antiseptics on streptococcus sanguinis.three antiseptic (chlorhexidine, olaflur, octenisept) and one putative antiadhesive (chitosan) agent were investigated for their effect on viable planktonic and attached streptococcus sanguinis cells. the bacterial pretreatment with each chemotherapeutic was performed in two steps: (i) after the exposure of planktonic streptococci to the antiseptics, the cells were suspended in human sterile saliva and allowed to attach to human enamel for 60 min; (ii) after 60 min in the flow chamber system, in ...200312648266
kuwanon g: an antibacterial agent from the root bark of morus alba against oral pathogens.kuwanon g was isolated from the ethyl acetate fraction of methanol extract of morus alba and its structure was elucidated by 13c-nmr, 1h-nmr and fab-ms. antibacterial activity of kuwanon g was investigated by the minimum inhibitory concentration (mic) test and the viable cell count method. mic of kuwanon g against streptococcus mutans causing dental caries was determined to be 8.0 microg/ml. the bactericidal test showed that kuwanon g completely inactivated s. mutans at the concentration 20 micr ...200312648813
prevalence of csh-like fibrillar surface proteins among mitis group oral streptococci.the prevalence of csh-like fibrillar surface proteins among oral streptococci was investigated by elisa and by immunoelectron microscopy using antiserum raised to recombinant fragments of csha of streptococcus gordonii dl1. the majority of s. gordonii, streptococcus sanguis and streptococcus oralis strains tested elaborated short (ca. 50-80 nm long) surface fibrils and reacted with antiserum to the amino acid repeat region of csha, demonstrating the widespread nature of csh-like proteins among t ...200312654102
homograft valve repair for recurrent prosthetic valve endocarditis. 200312658218
will triclosan in toothpaste select for resistant oral streptococci?the aims of the study were to determine the in vitro sensitivity of triclosan against viridans streptococci and the impact of triclosan on the normal oral microflora. nine subjects used a triclosan-containing toothpaste for 2 weeks. saliva samples were collected on days 0 and 14 and were analyzed quantitatively. the minimum inhibitory concentrations of triclosan and of several antimicrobial agents were determined for the streptococci isolated on days 0 and 14. no major changes occurred in the no ...200312667241
pathogenesis of adamantiades-behçet's disease.the aetiology of adamantiades-behçet's disease remains unknown and its pathogenesis is not fully understood. linked intrinsic and extrinsic factors are thought to contribute to the development of the disease, which probably occurs by environmental triggering of a genetically determined disorder. transmission is solely vertical, indicating that the disease is not contagious. genetic factors have been investigated and a significant link of hla-b51, especially of hla-b5101, has been identified. how ...200312684757
in vitro assay for the screening of the plaque-reducing activity of antimicrobial agents.bacteria grown in biofilms are less susceptible to antimicrobial agents than planktonic bacteria. one of the most common biofilms in humans is dental plaque. to investigate the antimicrobial activity against dental bacteria grown in biofilms, biofilms were generated with streptococcus sanguis on hydroxyapatite disks to mimic the tooth surface. after 48 h of aerobic incubation at 37 degrees c in a continuous flow culture, a biofilm consisting of streptococcus sanguis became visible on the surface ...200312705173
susceptibility of planktonic versus attached streptococcus sanguinis cells to chlorhexidine.the effect of chlorhexidine (chx) on the viability of streptococcus sanguinis was investigated in a preclinical biofilm model separately on cells in the planktonic or attached life form. saliva-coated human enamel and glass slides were exposed to the streptococci suspended in sterile saliva for 30 min and 60 min in the flow chamber system. the chx exposition was performed in two parts: pretreatment of the planktonic bacteria before their attachment to enamel or glass, and treatment of bacteria a ...200312709846
pcr method is essential for detecting mycobacterium tuberculosis in oral cavity samples.tuberculosis is a re-emerging infectious disease, and infection by mycobacterium tuberculosis has been increasing in immunocompromised hosts, including elderly persons. m. tuberculosis-infected persons may receive dental treatment. to evaluate the risk of m. tuberculosis infection in dental clinics, we examined the detection rates of m. tuberculosis in sample of mixed saliva, dental plaque, extracted teeth, caries lesions, and denture plaque by nested polymerase chain reaction (pcr). the detecti ...200312753466
classification of oral streptococci by two-dimensional gel electrophoresis with direct activity stain for glycosyltransferases.thirty eight strains of oral streptococci were divided into six types by two-dimensional gel electrophoresis (2-de) followed by glycosyltransferase (glt) activity stain: type 1, streptococcus mutans; type 2, streptococcus rattus; type 3, streptococcus sobrinus and streptococcus downei; type 4, streptococcus cricetus; type 5, streptococcus salivarius; and type 6, streptococcus sanguis, streptococcus oralis and streptococcus gordonii. in types 1, 2 and 5, two glucosyltransferases synthesizing wate ...200312753469
glucan-binding proteins of the oral streptococci.the synthesis of extracellular glucan is an integral component of the sucrose-dependent colonization of tooth surfaces by species of the mutans streptococci. in investigators' attempts to understand the mechanisms of plaque biofilm development, several glucan-binding proteins (gbps) have been discovered. some of these, the glucosyltransferases, catalyze the synthesis of glucan, whereas others, designated only as glucan-binding proteins, have affinities for different forms of glucan and contribut ...200312764072
non-mutans streptococci in patients receiving radiotherapy in the head and neck area.to study mutans and non-mutans streptococci in patients after radiotherapy of the head and neck.200312771501
real-time interaction of oral streptococci with human salivary components.oral streptococci are present in large numbers in dental plaque and several types interact with the enamel salivary pellicle to form a biofilm on tooth surfaces. the respective affinity of individual streptococci for salivary components has an influence on the etiologic properties of oral biofilm in the development of dental caries. we studied real-time biospecific interactions between oral streptococci and salivary components utilizing biosensor technology to analyze surface plasmon resonance. ...200312823797
brain abscess due to streptococcus sanguis. 200312865554
stress as a determinant of saliva-mediated adherence and coadherence of oral and nonoral microorganisms.the mucosal secretory proteins, such as the salivary proteins, play a key role in the acquisition and regulation of the mucosal microflora. most notably, some microorganisms utilize the host's secretory proteins to adhere to the mucosa; a first step in colonization and infection. the secretory proteins also influence colonization by affecting the binding among microorganisms, a process denoted as coadherence. previously we reported that acute stressors cause specific changes in saliva compositio ...200312883111
echocardiographic presentations of endocarditis, and risk factors for rupture of a sinus of valsalva in childhood.in recent years, the diagnosis of infective endocarditis has been enhanced by the use of echocardiography. we sought, therefore, to review its effect on the management of endocarditis in children.200312887073
enthalpy of interaction between coaggregating and non-coaggregating oral bacterial pairs--a microcalorimetric study.bacterial adhesion and coaggregation are involved in the development of oral biofilms, called dental plaque. although various techniques have already been used to study different aspects of these bacterial interactions, microcalorimetry has not yet been applied. this paper describes how isothermal reaction calorimetry can be employed to determine the enthalpy of coaggregation between two oral bacterial pairs. for most biological processes, the enthalpy tends to reach a minimum value, reflecting ...200314500015
the effect of passive ultrasonic activation of 2% chlorhexidine or 5.25% sodium hypochlorite irrigant on residual antimicrobial activity in root canals.ninety-four single-canal roots were prepared using the step-down technique. forty-two canals were irrigated with 2% chlorhexidine, 42 canals with 5.25% sodium hypochlorite (naocl), and 10 control canals with phosphate-buffered saline (pbs). the chlorhexidine and naocl groups were each then equally divided into a final irrigation group and a 1-min passive ultrasonic irrigation group. canals were enlarged with a parapost drill. the apical 3-5 mm was covered with nail polish. canals were rinsed wit ...200314503827
activity of human beta-defensin 3 alone or combined with other antimicrobial agents against oral bacteria.the in vitro activities of human beta-defensin 3 (hbd-3) alone or combined with lysozyme, metronidazole, amoxicillin, and chlorhexidine were investigated with the oral bacteria streptococcus mutans, streptococcus sanguinis, streptococcus sobrinus, lactobacillus acidophilus, actinobacillus actinomycetemcomitans, and porphyromonas gingivalis. hbd-3 showed bactericidal activity against all of the bacterial species tested. the bactericidal effect was enhanced when the peptide was used in combination ...200314506056
[construction of plant expression plasmid of chimera sbr-ct delta a1].the purpose of this study is to construct plant expression plasmid containing the gene encoding chimera sbr-ct delta a1.200314513580
[scanning electron microscope observation of morphological influence p-aminobenzoic acid (paba) on porphyromonas gingivalis].in order to investigate the effect of paba produced by streptococcus sanguis on microecological balance of subgingival plaque, different concentrations of paba were applied to see if it can influence the form and adherence of p. gingivalis.200314513582
[evaluation of the cario-static effect of nidus vespae on biofilm model in vitro].to evaluate the nidus vespae's cario-static effect on biofilm model in vitro.200314513591
[brain abscesses after extracranial infections of the head and neck area].the authors report on 20 immunocompetent patients with brain abscess after 12 cases of middle ear, seven tooth and a single frontal sinus infection. the clinical aspects, hematochemical and microbiological data, the role of imaging diagnostics (ct, mr) and the type of treatment are analysed. neurosurgery was performed on 17 patients (85%), eight of whom subsequently underwent evacuation of the primary source of infection (four mastoidectomies, two timpanoplasties, two tooth extractions). mastoid ...200314523535
effect of increasing silver content in phosphate-based glasses on biofilms of streptococcus sanguis.silver is a powerful antibacterial ion that may be useful for dealing with localized infections, such as periodontitis. however, the use of silver in this role could be significantly improved by the development of an effective means of delivery. phosphate-based glasses may provide a means of delivering the ions in a controlled manner. in our study, we have examined the effect of increasing silver content in phosphate-based glasses on biofilms of streptococcus sanguis. glasses of nominally the sa ...200314566780
the acid-tolerant microbiota associated with plaque from initial caries and healthy tooth surfaces.the intent of this study was to compare the inherent acid tolerance of bacteria in samples of dental plaque from tooth sites in subjects with and without initial caries. plaque was collected from approximal surfaces showing early enamel caries and from healthy tooth surfaces in the same subjects, as well as from enamel surfaces of caries-free individuals. in addition to plating on blood agar, the plaque samples were plated directly on non-selective solid agar medium buffered to ph 7.0, 6.0, 5.5, ...200314571116
synergistic inhibitory effect of cationic peptides and antimicrobial agents on the growth of oral streptococci.although chlorhexidine is one of the most efficacious antimicrobial agents used for the prevention of dental caries, side effects limit its application. the effects of gaegurin 6 (ggn6), an animal-derived cationic peptide, and its derivatives ptp6 and ptp12 on the growth of oral streptococci were investigated to assess the potential of these agents for use in the prevention of dental caries. the minimal inhibitory concentrations of the peptides for inhibition of the growth of oral streptococci ( ...200314571120
proteome analysis of oral pathogens.the oral environment contains diverse communities of micro-organisms including bacteria, fungi, protozoa, and viruses. studies of oral ecology have led to an appreciation of the complexity of the interactions that oral micro-organisms have with the host in both health and disease. despite this, diseases such as dental caries and periodontal diseases are still worldwide human ailments, resulting in a high level of morbidity and an economic burden to society. proteomics offers a new approach to th ...200314578497
review article: oral ulceration--aetiopathogenesis, clinical diagnosis and management in the gastrointestinal clinic.oral ulceration is a common complaint of patients attending out-patient clinics. the aim of this review is to provide the gastroenterologist with a differential diagnosis of oral ulceration, and a practical guide for the management of recurrent aphthous stomatitis, including topical and systemic therapy. the association of recurrent aphthous stomatitis with behçet's disease and other systemic disorders, including coeliac disease, is discussed. recent evidence concerning the immunopathogenesis of ...200314616160
a rapid procedure to ascertain the antimicrobial efficacy of oral care formulations.a rapid method examining the antimicrobial efficacy of oral care formulations with alamar blue, an oxidation-reduction dye with fluorescent end-points, is described. significant correlations between increasing viable plate counts of the oral bacteria actinomyces viscosus, streptococcus sanguis, streptococcus mutans and actinobacillus actinomycetemcomitans and increased alamar fluorescence were noted. metabolically active bacteria reduced alamar with the reduced dye found in the cell-free filtrat ...200314622343
susceptibility of oral bacteria to melaleuca alternifolia (tea tree) oil in vitro.the in vitro activity of melaleuca alternifolia (tea tree) oil against 161 isolates of oral bacteria from 15 genera was determined. minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) ranged from 0.003 to 2.0% (v/v). mic90 values were 1.0% (v/v) for actinomyces spp., lactobacillus spp., streptococcus mitis and streptococcus sanguis, and 0.1% (v/v) for prevotella spp. isolates of porphyromonas, prevotella and veillonella had the lowest mics and mbcs, and isolates ...200314622345
non-contact removal of coadhering and non-coadhering bacterial pairs from pellicle surfaces by sonic brushing and de novo adhesion.coadhesion between oral microbial pairs is an established factor in the spatiotemporal development and prevalence of mixed-species communities in early dental plaque in vivo. this study compares removal and de novo adhesion of pairs of coadhering and non-coadhering oral actinomyces and streptococci by sonic brushing on salivary pellicles in a non-contact mode as a function of the distance between the brush and the pellicle surface in vitro. first, actinomycetes were adhered to a pellicle surface ...200314632680
increased killing of staphylococci and streptococci by daptomycin compared with cefazolin and vancomycin in an in vitro peritoneal dialysate model.peritoneal dialysate fluid (pdf) is a bacteriostatic medium that compromises the antibacterial activity of cell wall-active agents. by use of an in vitro static model, methicillin-resistant staphylococcus aureus (mrsa), methicillin-susceptible s. aureus (mssa), methicillin-susceptible staphylococcus epidermidis (msse), and streptococcus sanguis were exposed to daptomycin at concentrations of 10, 30, and 100 mg/liter, cefazolin at 125 mg/liter, and vancomycin at 25 mg/liter in cation-adjusted mue ...200314638479
in vitro cariogenic potential of candida albicans.the adherence and dissociation of candida albicans, c. tropicalis, streptococcus mutans and s. sanguis to six substrates including hydroxylapatite (hap) which exhibit various hydrophobicity, was examined by the use of a bioluminescent adenosine triphosphate (atp) assay. dissolution of hap by c. albicans or s. mutans was determined spectrophotometrically by the use of o-cresolphthalein complexone. in the adherence of c. tropicalis, s. mutans and s. sanguis, the amount of adherent cells correlated ...200314641620
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