Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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comparative antimicrobial efficacy of eucalyptus galbie and myrtus communis l. extracts, chlorhexidine and sodium hypochlorite against enterococcus faecalis. | the aim of this study was to evaluate the antimicrobial effect of eucalyptusgalbie and myrtus communis l. methanolic extracts, chlorhexidine (chx) and sodium hypochlorite (naocl) on enterococcus faecalis (e. faecalis) as the predominant species isolated from infected root canals. | 2017 | 28512487 |
comparative evaluation of three herbal solutions on the disinfection of guttapercha cones: an in vitro study. | guttapercha (gp) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. supplementary decontamination of gp cones is critical. as they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. | 2017 | 28511502 |
rapid identification of vancomycin resistant enterococcus faecalis clinical isolates using a sugar fermentation method. | vancomycin resistant enterococci (vre) can be found all over the world. thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. | 2017 | 28511382 |
development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens. | to develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (rpa) assays for detection of common bacterial urinary tract infection (uti) pathogens. | 2017 | 28510991 |
antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in saudi arabia. | premna resinosa (hochst.) schauer (lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. | 2017 | 28508699 |
the effect of sodium hypochlorite concentration and irrigation needle extension on biofilm removal from a simulated root canal model. | to investigate the effect of sodium hypochlorite concentration and needle extension on removal of enterococcus faecalis biofilm, sixty root canal models were 3d printed. biofilms were grown on the apical 3 mm of the canal for 10 days. irrigation for 60s with 9 ml of either 5.25% or 2.5% naocl or water was performed using a needle inserted either 3 or 2 mm from the canal terminus and imaged using fluorescence microscopy and residual biofilm percentages were calculated using imaging software. the ... | 2017 | 28508523 |
empyema thoracis caused by an optra-positive and linezolid-intermediate enterococcus faecalis strain. | 2017 | 28506787 | |
chemical composition, antibacterial, schistosomicidal, and cytotoxic activities of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae). | we have investigated the chemical composition and the antibacterial activity of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae) (da-eo) against a representative panel of cariogenic bacteria. we have also assessed the in vitro schistosomicidal effects of da-eo on schistosoma mansoni and its cytotoxicity to gm07492-a cells in vitro. gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms) revealed that the monoterpenes cis-piperitone oxide (35. ... | 2017 | 28504841 |
metabolic impact of nutrient starvation in mevalonate-producing escherichia coli. | the aim of this work was to enhance mevalonate yield from glucose in escherichia coli by essential nutrient starvations and to reveal these effects on the central carbon metabolism. stationary phase culture without essential nutrients such as nitrogen, sulfur, and magnesium was evaluated using an engineered e. coli introducing mvae and mvas genes from enterococcus faecalis. sulfur starvation resulted in the highest mevalonate yield of 0.61c-molc-mol(-1) from glucose. the metabolic impacts of nut ... | 2017 | 28501379 |
occurrence of ampicillin-resistant enterococcus faecium carrying esp gene in pet animals: an upcoming threat for pet lovers. | this study was carried out to investigate oral colonisation by enterococcus faecalis and enterococcus faecium in pet dogs and cats, with special reference to antibiotic resistance. | 2017 | 28499907 |
an iterative glycosyltransferase ents catalyzes transfer and extension of o- and s-linked monosaccharide in enterocin 96. | glycosyltransferases are essential tools for in vitro-glycoengineering. bacteria harbor an unexplored variety of protein glycosyltransferases. here, we describe a peptide glycosyltransferase (ents) encoded by orf0417 of enterococcus faecalis tx0104. ents di-glycosylates linear peptide of enterocin 96- a known antibacterial, in vitro. it is capable of transferring as well as extending the glycan onto the peptide in an iterative sequential dissociative manner. it can catalyze multiple linkages: gl ... | 2017 | 28498962 |
etiological and resistance profile of bacteria involved in urinary tract infections in young children. | background. the objective of this study was to identify the bacteria most frequently responsible for urinary tract infection (uti) in the population of under-2-year-olds in our geographic area and to evaluate the activity of antibiotics widely used for uti treatment during a 4-year study period. materials and methods. a retrospective analysis was conducted of data on the identification and susceptibility of microorganisms isolated in urine samples from children under 2 years of age. results. a t ... | 2017 | 28497052 |
antimicrobial resistance patterns in women with positive urine culture: does menopausal status make a significant difference? | aim. urinary tract infection (uti) is considered one of the most common bacterial infections in women. the aim of this study was to investigate the types of uropathogens present, as well as the degree of antimicrobial drug resistance seen among premenopausal (n = 2748) and postmenopausal (n = 1705) women with uncomplicated uti. methods. urinary samples (n = 4453) collected from women with uti were analyzed in terms of uropathogens present. these were considered as positive if bacterial growth wa ... | 2017 | 28497048 |
evaluation of the synergistic effect of tomatidine with several antibiotics against standard and clinical isolates of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. | antibiotic resistance is an important problem in antibiotic treatment of infections, particularly in hospitals. tomatidine is a plant secondary metabolite with antimicrobial and antifungal effects. this study examined the possible synergistic effect tomatidine with several antibiotics against standard and clinical strains of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. after determining the minimum inhibitory concentrations (mics) of antibiotics and ... | 2017 | 28496482 |
chemical composition, antibacterial and antifungal activities of flowerhead and root essential oils of santolina chamaecyparissus l., growing wild in tunisia. | the antimicrobial properties of essential oil from various santolina species have not been investigated enough in the previous studies dealing with the biological activities of medicinal plants. in tunisia, santolina chamaecyparissus l. (asteraceae) is the only santolina species recorded and is used as vermifuge and emmenagogue. the chemical composition, antibacterial and antifungal properties of essential oils from the flowerheads and roots of spontaneous s. chamaecyparissus growing in tunisia ... | 2017 | 28490960 |
the most frequent isolates from outpatients with urinary tract infection. | the main objective of the study was to identify the most frequent isolates from female outpatients with urinary tract infection. | 2017 | 28484348 |
five-year summary of in vitro activity and resistance mechanisms of linezolid against clinically important gram-positive cocci in the united states from the leader surveillance program (2011 to 2015). | this report describes linezolid susceptibility testing results for 6,741 gram-positive pathogens from 60 u.s. sites collected during 2015 for the leader program. in addition, the report summarizes linezolid in vitro activity, resistance mechanisms, and molecular typing obtained for 2011 to 2015. during 2015, linezolid showed potent activity in testing against staphylococcus aureus, inhibiting >99.9% of 3,031 isolates at ≤2 µg/ml. similarly, linezolid showed coverage against 99.2% of coagulase-ne ... | 2017 | 28483950 |
association of metal homeostasis and (p)ppgpp regulation in the pathophysiology of enterococcus faecalis. | in enterococcus faecalis, the regulatory nucleotides pppgpp and ppgpp, collectively, (p)ppgpp, are required for growth in blood, survival within macrophages, and virulence. however, a clear understanding of how (p)ppgpp promotes virulence in e. faecalis and other bacterial pathogens is still lacking. in the host, the essential transition metals iron (fe) and manganese (mn) are not readily available to invading pathogens because of a host-driven process called nutritional immunity. considering it ... | 2017 | 28483855 |
cytoprotective effect of the enzyme-mediated polygallic acid on fibroblast cells under exposure of uv-irradiation. | the poly(gallic acid), produced by laccase-mediated oxidation of gallic acid in aqueous media (ph5.5) to attain a novel material with well-defined molecular structure and high water solubility (500mg/ml at 25°c), has been investigated to understand its potential biological activities. in this regard, a biomedical approach based on cytoprotective effect on human fibroblast cells exposed to uv-irradiation in the presence of the polymer has been demonstrated. the results also shows that 200μg/ml of ... | 2017 | 28482545 |
use of photo-fenton's reaction by 400-nm led light for endodontic disinfection: a preliminary in vitro study on enterococcus faecalis. | one of the biggest challenges in endodontics is the complete disinfection of root canals. in addition to mechanical preparation, the technique traditionally also involves channel disinfection with other agents such as sodium hypochlorite, hydrogen peroxide, chlorhexidine, or a combination of these. some bacterial species are particularly resistant to eradication. using enterococcus faecalis in this preliminary study, we tested the bactericidal effectiveness of the fenton reaction and the photo-f ... | 2017 | 28482224 |
daptomycin: new insights into an antibiotic of last resort. | 2017 | 28481142 | |
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds. | natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars. | 2017 | 28480436 |
does the removal of cementum facilitate bacterial penetration into dentinal tubules in vitro? | the importance of an intact layer of cementum on the root surface in preventing bacterial penetration into radicular dentin has not been sufficiently investigated. the aim of this in vitro study was to determine the effect of the absence of cementum from the root surface and the length of the infection period (2 or 4 weeks) on the maximum depth of bacterial penetration and the percentage of sectors lined with bacteria. | 2017 | 28476468 |
urinary tract infections in postmenopausal women with type 2 diabetes: clinical correlates and quinolone susceptibility. | introduction diabetes mellitus and the postmenopausal period are associated with increased risk of urinary tract infections (utis) in women. however, data on utis in postmenopausal diabetic women are scarce. objectives the aim of this study was to determine the prevalence of utis in postmenopausal women with type 2 diabetes mellitus, identify the potential risk factors, describe the causative pathogens, and assess their susceptibility to quinolones. patients and methods patients were interviewe ... | 2017 | 28475170 |
a short artificial antimicrobial peptide shows potential to prevent or treat bone infections. | infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. antimicrobial peptides (amps) are naturally occurring peptides and promising candidates for treatment of joint infections. this study aimed to analyze the effect of short artificial peptides derived from an optimized library regarding (1) antimicrobial effect on different bacterial species, (2) efficac ... | 2017 | 28473710 |
chicken embryo lethality assay for determining the lethal dose and virulence of enterococcus faecalis. | enterococcus faecalis is the major pathogen found in field cases of amyloid arthropathy in chickens. given the need for a better understanding of the virulence mechanisms of the causative strains, the embryo lethality assay (ela) is proposed in the present study as a model to evaluate the virulence of e. faecalis strains, specifically the pathogenic avian strain k923/96, which was previously related with amyloid arthropathy. hence, 0.2 ml of five doses of the cited strain (from 2.5 to 2500 colon ... | 2017 | 28470098 |
evaluation of antibacterial efficacy of photodynamic therapy vs. 2.5% naocl against e. faecalis-infected root canals using real-time pcr technique. | bacteria like e. faecalis can produce intra- and extra-radicular biofilms. theoretically, the adjustable penetration ability of lasers enables better access to root canal system. therefore the aim of the present study was to compare the ability of photoactivated laser and 2.5% naocl irrigation solution to eliminate e. faecalis from the root canals by real-time pcr technique. | 2017 | 28469819 |
role of surface energy and nano-roughness in the removal efficiency of bacterial contamination by nonwoven wipes from frequently touched surfaces. | healthcare associated infections (hcais) are responsible for substantial patient morbidity, mortality and economic cost. infection control strategies for reducing rates of transmission include the use of nonwoven wipes to remove pathogenic bacteria from frequently touched surfaces. wiping is a dynamic process that involves physicochemical mechanisms to detach and transfer bacteria to fibre surfaces within the wipe. the purpose of this study was to determine the extent to which systematic changes ... | 2017 | 28469734 |
molecular mechanism of quorum-sensing in enterococcus faecalis: its role in virulence and therapeutic approaches. | quorum-sensing systems control major virulence determinants in enterococcusfaecalis, which causes nosocomial infections. the e. faecalis quorum-sensing systems include several virulence factors that are regulated by the cytolysin operon, which encodes the cytolysin toxin. in addition, the e. faecalis fsr regulator system controls the expression of gelatinase, serine protease, and enterocin o16. the cytolysin and fsr virulence factor systems are linked to enterococcal diseases that affect the hea ... | 2017 | 28467378 |
total staphylococci as performance surrogate for greywater treatment. | faecal indicator bacteria (fib) are commonly used as water quality indicators; implying faecal contamination and therefore the potential presence of pathogenic enteric bacteria, viruses, and protozoa. hence in wastewater treatment, the most commonly used treatment process measures (surrogates) are total coliforms, faecal coliforms, escherichia coli (e. coli), and enterococci. however, greywater potentially contains skin pathogens unrelated to faecal load, and e. coli and other fib may grow withi ... | 2017 | 28462431 |
applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) can be used to identify pathogens in blood culture samples. however, sample pretreatment is needed for direct identification of microbes in blood culture bottles. conventional protocols are complex and time-consuming. therefore, in this study, we developed a method for collecting bacteria using polyallylamine-polystyrene copolymer for application in wastewater treatment technology. using representative ba ... | 2017 | 28461023 |
bacterial diversity of the gastric content of preterm infants during their first month of life at the hospital. | studies focused on the stomach microbiota are relatively scarce, and most of them are focused on the adult population. the aim of this work is to describe the bacterial communities inhabiting the gastric content (gc) of preterm neonates. for that purpose, gc samples were collected weekly from a total of 13 preterm neonates during their first month of life within their hospital stay. samples were analyzed by using both culture-dependent and -independent techniques. the former allowed the isolatio ... | 2017 | 28459051 |
transcriptomics analysis reveals putative genes involved in biofilm formation and biofilm-associated drug resistance of enterococcus faecalis. | enterococcus faecalis is a gram-positive bacterium associated with endodontic infections and is capable of forming biofilms that can confer drug resistance to the bacterium, resulting in treatment failure. current knowledge on e. faecalis drug resistance is of a limited and conflicting nature. the present study examined the genetic basis of e. faecalis biofilm formation and drug resistance using a rna sequencing (rna-seq)-based transcriptome approach. | 2017 | 28457636 |
high-purity nisin alone or in combination with sodium hypochlorite is effective against planktonic and biofilm populations of enterococcus faecalis. | nisin, a broad-spectrum bacteriocin, has recently been highlighted for its biomedical applications. to date, no studies have examined the antimicrobial and antibiofilm properties of high-purity (>95%) nisin (nisin zp) on enterococcus faecalis and biofilms formed by this species. we hypothesize that nisin can inhibit e. faecalis and reduce biofilm biomass, and combinations of nisin and sodium hypochlorite (naocl) will enhance the antibiofilm properties against e. faecalis biofilms. | 2017 | 28457635 |
enzymes required for maltodextrin catabolism in enterococcus faecalis exhibit novel activities. | maltose and maltodextrins are formed during the degradation of starch or glycogen. maltodextrins are composed of a mixture of maltooligosaccharides formed by α-1,4- but also some α-1,6-linked glucosyl residues. the α-1,6-linked glucosyl residues are derived from branching points in the polysaccharides. in enterococcus faecalis, maltotriose is mainly transported and phosphorylated by a phosphoenolpyruvate:carbohydrate phosphotransferase system. the formed maltotriose-6″-phosphate is intracellular ... | 2017 | 28455338 |
response surface method optimization of a novel hypericin formulation in p123 micelles for colorectal cancer and antimicrobial photodynamic therapy. | the photodynamic properties of hypericin (hyp) may be used as an alternative treatment for malignancies of the lower gastrointestinal tract and for the prevention of surgical-site infection; however, its use in photodynamic therapy has been limited because of its poor hydrosolubility. therefore, in order to improve its water solubility and its photodynamic effect, hyp was encapsulated in pluronic p123 (p123) and the photodynamic effects against intestinal and epidermal bacteria and against two l ... | 2017 | 28454049 |
recurrence of deep surgical site infection in cerebral palsy after spinal fusion is rare. | retrospective review of prospective registry. | 2017 | 28449964 |
synthesis and bioactivity evaluation of novel 2-salicyloylbenzofurans as antibacterial agents. | in order to discover new antibacterial agents, series of 2-salicyloylbenzofuran derivatives were designed, synthesized and evaluated for their antibacterial activities against three gram-(+) strains (methicillin-sensitive staphylococcus aureus (mssa) atcc 29213, methicillin-resistant staphylococcus aureus (mrsa) atcc 43300, and streptococcus faecalis (s. faecalis) atcc 29212) and one gram-(-) strain (escherichia coli (e. coli) atcc 25922). the 2-salicyloylbenzofuran heterocycles were generated b ... | 2017 | 28441350 |
substance p enhances lactic acid and tyramine production in enterococcus faecalis v583 and promotes its cytotoxic effect on intestinal caco-2/tc7 cells. | enterococcus faecalis, generally considered as a saprophytic bowel commensal, has recently emerged as an important nosocomial pathogen causing severe urinary tract infections, surgical wound infections, bacteremia, and bacterial endocarditis. this bacterium is capable of forming biofilms on various surfaces and its high level of antibiotic resistance contributes to its pathogenicity. the aim of this study was to evaluate the effect on e. faecalis, of substance p (sp), an antimicrobial peptide th ... | 2017 | 28439299 |
comparison of linezolid and vancomycin lock solutions with and without heparin against biofilm-producing bacteria. | the activity of linezolid and vancomycin lock solutions against biofilm-producing strains of staphylococcus aureus, s. epidermidis, and enterococcus faecalis was studied. | 2017 | 28438824 |
comparative evaluation of antimicrobial efficacy of an alternative natural agent for disinfection of toothbrushes. | the aim of this study was to assess the antimicrobial potency of aqueous extract of psidium guajava leaves in two different concentrations as a toothbrush disinfectant against three oral bacterial species. | 2017 | 28435376 |
dual-targeting small-molecule inhibitors of the staphylococcus aureus fmn riboswitch disrupt riboflavin homeostasis in an infectious setting. | riboswitches are bacterial-specific, broadly conserved, non-coding rna structural elements that control gene expression of numerous metabolic pathways and transport functions essential for cell growth. as such, riboswitch inhibitors represent a new class of potential antibacterial agents. recently, we identified ribocil-c, a highly selective inhibitor of the flavin mononucleotide (fmn) riboswitch that controls expression of de novo riboflavin (rf, vitamin b2) biosynthesis in escherichia coli. he ... | 2017 | 28434876 |
disinfection of water inoculated with enterococcus faecalis using solar/fe(iii)edds-h2o2 or s2o8(2-) process. | in this study, the activation of h2o2 and persulfate ions induced by solar photolysis of fe(iii)edds complex were investigated in water disinfection, applying solar aops processes. the use of fe(iii)edds complex maintains iron in soluble form until slightly basic ph and so the photolysis is efficient in a large range of ph compatible with natural waters. moreover, for the first time, the impact of photogenerated hydroxyl and sulfate radicals on the inactivation of enterococcus faecalis in water ... | 2017 | 28433695 |
diversity of integrative and conjugative elements of streptococcus salivarius and their intra- and interspecies transfer. | integrative and conjugative elements (ices) are widespread chromosomal mobile genetic elements which can transfer autonomously by conjugation in bacteria. thirteen ices with a conjugation module closely related to that of icest3 of streptococcus thermophilus were characterized in streptococcus salivarius by whole-genome sequencing. sequence comparison highlighted ice evolution by shuffling of 3 different integration/excision modules (for integration in the 3' end of the fda, rpsi, or rpmg gene) ... | 2017 | 28432093 |
[effects of application of vancomycin in the early stage of patients with extremely severe burn]. | objective: to evaluate the effects of application of vancomycin in the early stage of patients with extremely severe burn, in order to provide reference to drug for anti-infection treatment in the early stage of patients with extremely severe burn. methods: data of 15 patients of kunshan explosion on august 2nd, 2014, admitted to the department of intensive care in our hospital were retrospectively analyzed. the clinical efficacy of continuously intravenous dripping of vancomycin (combined with ... | 2017 | 28427133 |
correction: chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against enterococcus faecalis biofilms. | [this corrects the article doi: 10.1371/journal.pone.0174888.]. | 2017 | 28426772 |
comprehensive analysis of phospholipids and glycolipids in the opportunistic pathogen enterococcus faecalis. | enterococcus faecalis is a gram-positive, opportunistic, pathogenic bacterium that causes a significant number of antibiotic-resistant infections in hospitalized patients. the development of antibiotic resistance in hospital-associated pathogens is a formidable public health threat. in e. faecalis and other gram-positive pathogens, correlations exist between lipid composition and antibiotic resistance. resistance to the last-resort antibiotic daptomycin is accompanied by a decrease in phosphatid ... | 2017 | 28423018 |
split tasks of asymmetric nucleotide-binding sites in the heterodimeric abc exporter efrcd. | many heterodimeric atp-binding cassette (abc) exporters evolved asymmetric atp-binding sites containing a degenerate site incapable of atp hydrolysis due to noncanonical substitutions in conserved sequence motifs. recent studies revealed that nucleotide binding to the degenerate site stabilizes contacts between the nucleotide-binding domains (nbds) of the inward-facing transporter and regulates atp hydrolysis at the consensus site via allosteric coupling mediated by the d-loops. however, it is u ... | 2017 | 28417533 |
effect of instrumentation techniques and preparation taper on apical extrusion of bacteria. | the aim of this in vitro study was to evaluate the effects of different root canal instrumentation techniques and preparation tapers on the amount of apically extruded bacteria. | 2017 | 28416303 |
effect of bacterial biofilm on the osteogenic differentiation of stem cells of apical papilla. | although clinical success in regenerative endodontics is substantially high, histological success is limited to finding bone/cementum-like tissue instead of dentin within the canal space. the aims of this study were to investigate (1) the effect of bacterial biofilm on osteogenic gene expression in stem cells of the apical papilla (scap) and (2) the effect of bacterial antigens on the functional differentiation of scap into a mineralizing phenotype. | 2017 | 28416302 |
bacterial epidemiology and antimicrobial resistance profiles of urinary specimens of the elderly. | although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles. | 2017 | 28408060 |
preventive effects of heat-killed enterococcus faecalis strain ec-12 on mouse intestinal tumor development. | establishing effective methods for preventing colorectal cancer by so-called "functional foods" is important because the global burden of colorectal cancer is increasing. enterococcus faecalis strain ec-12 (ec-12), which belongs to the family of lactic acid bacteria, has been shown to exert pleiotropic effects, such as anti-allergy and anti-infectious effects, on mammalian cells. in the present study, we aimed to evaluate the preventive effects of heat-killed ec-12 on intestinal carcinogenesis. ... | 2017 | 28406434 |
peptidoglycan o-acetylation increases in response to vancomycin treatment in vancomycin-resistant enterococcus faecalis. | vancomycin resistance is conferred upon vancomycin-resistant enterococci (vre) through the replacement of peptidoglycan (pg) stem terminal d-ala-d-ala with d-ala-d-lac. the d-ala-d-lac incorporation can affect both the fitness and virulence of vre. here we comprehensively investigate the changes to pg composition in vancomycin-resistant enterococcus faecalis following the growth in presence of vancomycin using liquid chromatography-mass spectrometry. using high-resolution mass spectrometry, 104 ... | 2017 | 28406232 |
rna and a cell wall component of enterococcus faecalis ic-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line j774.1. | enterococcus faecalis is a resident lactic acid bacterium in the human intestine. its immunostimulatory action was reported to be enhanced by heat sterilization. to investigate its beneficial actions, we evaluated the ability of 10 e. faecalis strains to induce interleukin-12 (il-12) production in a mouse macrophage cell line, j774.1 and found that the strain, e. faecalis ic-1, had a potent il-12-inducing ability. furthermore, we investigated the underlying mechanism by treating ic-1 cells with ... | 2017 | 28406067 |
antibiotic resistant airborne bacteria and their multidrug resistance pattern at university teaching referral hospital in south ethiopia. | hospitals provide a reservoir of microorganisms, many of which are multi-resistant to antibiotics. emergence of multi-drug resistant strains in a hospital environment, particularly in developing countries is an increasing problem to infection treatment. this study aims at assessing antibiotic resistant airborne bacterial isolates. | 2017 | 28403894 |
strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic lactobacillus spp. | urinary tract infections (utis), one of most common infections worldwide, face high recurrence rates and increasing antimicrobial resistance. probiotic bacteria, especially of the genus lactobacillus, are considered a promising preventive and/or treatment therapy against utis. in order to elucidate the mechanisms involved in these beneficial effects, we studied the impact of different lactobacillus strains (lactobacillus salivarius ucm572, l. plantarum clc17 and l. acidophilus 01) in the adheren ... | 2017 | 28402532 |
wound infection secondary to snakebite. | snakebites can produce severe local and systemic septic complications as well as being associated with significant overall morbidity and even mortality. | 2017 | 28395682 |
stochasticity among antibiotic-resistance profiles of common burn-related pathogens over a six-year period. | one of the most significant contributors to morbidity and death in patients with burns is infection, which accounts for 30%-75% of post-burn fatalities. because of concerns for the development of antibiotic resistance in burn-related pathogens, the aims of this study were to identify antibiotic resistance trends for the four most common burn-related pathogens over a six-year period. | 2017 | 28394746 |
examination of enterococcus faecalis toxin-antitoxin system toxin fst function utilizing a pheromone-inducible expression vector with tight repression and broad dynamic range. | tools for regulated gene expression in enterococcus faecalis are extremely limited. in this report, we describe the construction of an expression vector for e. faecalis, designated pcie, utilizing the pq pheromone-responsive promoter of plasmid pcf10. we demonstrate that this promoter is tightly repressed, responds to nanogram quantities of the peptide pheromone, and has a large dynamic range. to demonstrate its utility, the promoter was used to control expression of the toxic peptides of two pa ... | 2017 | 28348028 |
yeasts harbored by vespine wasps in the pacific northwest. | the ecological role of social wasps has been extensively studied, but little is known about symbiotic relationships of these wasps with microbes. recently, it was shown that vespid wasps in europe carry yeasts, predominantly saccharomyces cerevisiae, in their gastrointestinal (gi) tract. interestingly, this niche allowed for sexual recombination of yeasts to occur and the formation of novel hybrid species. our goals were 1) to survey the gi tract of eusocial wasps in the pacific northwest for th ... | 2017 | 28169397 |
synthesis and antimicrobial activity of chiral quaternary n-spiro ammonium bromides with 3',4'-dihydro-1'h-spiro[isoindoline-2,2'-isoquinoline] skeleton. | a new class of highly functionalized tetrahydroisoquinolines with a quaternary carbon stereocenter was synthesized starting from an easily accessible l-tartaric acid. nine strains of bacteria (staphylococcus aureus, streptococcus pyogenes, streptococcus mutans, streptococcus salivarius, bacillus subtilis, enterococcus faecalis, moraxella catarrhalis, escherichia coli, campylobacter jejuni) were used for the determination of minimal inhibitory concentration (mic) and minimal bactericidal concentr ... | 2017 | 28740363 |
a prospective randomised trial of isolated pathogens of surgical site infections (ssi). | every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported. | 2017 | 28761643 |
implementation of the new virtuo blood culture system: evaluation and comparison to the 3d system using simulated blood cultures. | to evaluate the performances of the newly approved bact/alert virtuo blood culture system for the recovery of bloodstream pathogens and compare it to the bact/alert 3d system. | 2017 | 28583022 |
comparative antimicrobial efficacy of selected root canal irrigants on commonly isolated microorganisms in endodontic infection. | this study aims to evaluate and compare the antimicrobial efficacy of three selected root canal irrigants (biopure mtad, metronidazole, aztreonam) against microbes commonly isolated from polymicrobial microbiota of root canal infection. | 2017 | 28435359 |
azathioprine, mercaptopurine, and 5-aminosalicylic acid affect the growth of ibd-associated campylobacter species and other enteric microbes. | campylobacter concisus is a bacterium that is associated with inflammatory bowel disease (ibd). immunosuppressive drugs including azathioprine (aza) and mercaptopurine (mp), and anti-inflammatory drug such as 5-aminosalicylic acid (5-asa) are commonly used to treat patients with ibd. this study aimed to examine the effects of aza, mp, and 5-asa on the growth of ibd-associated bacterial species and to identify bacterial enzymes involved in immunosuppressive drug metabolism. a total of 15 bacteria ... | 2017 | 28424670 |
efficacy and safety of probiotic-supplemented triple therapy for eradication of helicobacter pylori in children: a systematic review and network meta-analysis. | the aim of this study was to identify the best probiotic supplementation in triple therapy for pediatric population with helicobacter pylori infection. | 2017 | 28681177 |
[bifidobacterium scardovii isolated from 2 consecutive urine samples]. | bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by co2 and anaerobiosis. exceptionally it has been associated with infections in humans. an elderly male patient with a urinary tract infection due to b. scardovii and enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. the bacillus did not grow on standard media, but on chocolate agar incubated in co2 and on supplemented bru ... | 2017 | 28734711 |
in vitro evaluation of cytotoxic, anti-proliferative, anti-oxidant, apoptotic, and anti-microbial activities of cladonia pocillum. | the aim of this study was to investigate the anti-proliferative, apoptotic, cytotoxic, and anti-oxidant effects of extracts from the lichen cladonia pocillumon human breast cancer cells (mcf-7), and to characterize the anti-microbial features. mcf-7 cells were treated with methanolic c. pocillum extract for 24h. the cytotoxicity of the extract was tested with mtt. moreover, its anti-proliferative effects were examined with immunocytochemical method. apoptosis and biochemical parameters were det ... | 2017 | 28838343 |
investigation of effect of 1,8-cineole on antimicrobial activity of chlorhexidine gluconate. | chlorhexidine belongs to a group of medicines called antiseptic antibacterial agents. chlorhexidine is commonly used for the care and clean off the skin, hands, and wounds. in recent years, medicinal and aromatic plants have been used for prevention of disease, maintaining health, and improving disease in traditional and modern medicine as a medicament. according to recent research, cineole is the isolated active agent of eucalyptus oil and possesses antimicrobial activity. it was demonstrated t ... | 2017 | 28827963 |
proinflammatory mg-63 cells response infection with enterococcus faecalis cps2 evaluated by the expression of tlr-2, il-1β, and inos mrna. | we have previously demonstrated that unencapsulated enterococcus faecalis cps2 inhibits biofilm formation of candida albicans, a fungus commonly found with e. faecalis in periapical lesion. in this study, we compared encapsulated and unencapsulated e. faecalis cps2 strains relationship with osteoblastic (mg-63) cells, whereas e. faecalis atcc 29212 were used as a reference strain. | 2017 | 28800779 |
antibacterial and antifungal activity of endodontic intracanal medications. | the sterilization of the entire root canal system represents the main goal of every endodontist, given the fact that the control of the microbial flora is the key point of every root canal treatment. the diversity of microorganisms found inside the root canal and also the resistance of some bacterial species to intracanal medications led to a continuous development of new endodontic products. the present study focuses on the comparison of the antibacterial and antifungal properties of different ... | 2017 | 28781531 |
antimicrobial and immunomodulatory activity of host defense peptides, clavanins and ll-37, in vitro: an endodontic perspective. | endodontic treatment is mainly based on root canal disinfection and its failure may be motivated by microbial resistance. endodontic therapy can be benefitted by host defense peptides (hdps), which are multifunctional molecules that act against persistent infection and inflammation. this study aimed to evaluate the antimicrobial, cytotoxic and immunomodulatory activity of several hdps, namely clavanin a, clavanin a modified (mo) and ll-37, compared to intracanal medication ca(oh)2. hdps and ca(o ... | 2017 | 28712894 |
thymus vulgaris l. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity. | this study evaluated the biological effects of the t. vulgaris l. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. | 2017 | 28683409 |
comparison of the antimicrobial efficacy of calcium hydroxide and photodynamic therapy against enterococcus faecalis and candida albicans in teeth with periapical lesions; an in vivo study. | introduction: elimination of pathological microflora of root canal systems is a major goal in endodontic treatment. this study aimed to compare the antimicrobial efficacy of calcium hydroxide as an intracanal medication and antibacterial photodynamic therapy (apdt) against enterococcus faecalis and candida albicans in teeth with periapical (pa) lesions. methods: this in vivo study was conducted on 20 patients with single-rooted mandibular premolar with previously failed endodontic treatment. thi ... | 2017 | 28652899 |
in vitro evaluation of antimicrobial activity of alimentary canal extracts from the red palm weevil, rhynchophorus ferrugineus olivier larvae. | the invasive red palm weevil, rhynchophorus ferrugineus olivier (coleoptera: curculionidae), is considered one of the world's most devastating insect pests to palm trees. it was observed that larvae of this pest are able to inhibit microbial growth on the rearing media when they start feeding and this observation has led us to study the effect of red palm weevils on various microbial species. the antimicrobial effect of extracts from different parts of the alimentary canal on gram positive bacte ... | 2017 | 28612029 |
formulation and evaluation of sol-gel drug delivery system for intracanal ph sensitive controlled delivery of chlorhexidine. | persistence of viable micro-organisms even after thorough chemomechanical debridement has been cited as a major cause for endodontic failure. chlorhexidine is a drug, which has shown marked efficacy against enterococcus faecalis and candida spp., which are mostly accounted for endodontic failure and it has demonstrated high degree of substantivity to dentin by adsorption. another issue with chlorhexidine and other intracanal medicaments is the excessive or premature peaking of the drug leading t ... | 2017 | 28571266 |
antimicrobial and anti-biofilm properties of polypropylene meshes coated with metal-containing dlc thin films. | a promising strategy to reduce nosocomial infections related to prosthetic meshes is the prevention of microbial colonization. to this aim, prosthetic meshes coated with antimicrobial thin films are proposed. commercial polypropylene meshes were coated with metal-containing diamond-like carbon (me-dlc) thin films by the magnetron sputtering technique. several dissimilar metals (silver, cobalt, indium, tungsten, tin, aluminum, chromium, zinc, manganese, tantalum, and titanium) were tested and com ... | 2017 | 28560581 |
application of hyperosmotic nanoemulsions in wound healing: partial thickness injury model in swine. | objective: in this work, we introduce a novel hyperosmotic nanoemulsion (hne) topical agent for use in wound healing. these topical emulsion complexes combine a lipophilic thymol nanoemulsion with a hyperosmotic saccharide matrix. this combination has been previously shown to possess synergistic antimicrobial activity against a host of common and drug-resistant pathogens in vitro. approach: in this study, we present additional data to assess the safety and efficacy of these emulsions in a partia ... | 2017 | 28507786 |
microbiological analysis of acute infections of the nail fold on the basis of bait thread test. | an acute infection of the nail fold, called paronychia, is a common clinical problem. the basis for the implementation of the treatment is the result of microbiological examination. due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. | 2017 | 28507488 |
comparative evaluation of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, and a proton pump inhibitor against resistant root canal pathogens. | the objective of this study is to compare the antimicrobial efficacy of triple antibiotic paste (tap) and a proton pump inhibitor (ppi) (omeprazole) in combination with calcium hydroxide (ch) against enterococcus faecalis and candida albicans. | 2017 | 28435366 |
efficacy of three different lasers on eradication of enterococcus faecalis and candida albicans biofilms in root canal system. | the objective was to compare the efficacy of three different lasers in disinfection of root canals inoculated with enterococcus faecalis and candida albicans biofilms. | 2017 | 28426292 |
antimicrobial photodynamic therapy against endodontic enterococcus faecalis and candida albicans mono and mixed biofilms in the presence of photosensitizers: a comparative study with classical endodontic irrigants. | endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. in this study, it was assessed the efficacy of antimicrobial photodynamic therapy (apdt) with the zn(ii)chlorin e6 methyl ester (zn(ii)e6me) activated by red light against monospecies and mixed biofilms of enterococcus faecalis and candida albicans. the results were compared with the ones obtained with rose bengal (rb), toluidine blue-o (tbo), the synthetic tetracationic porphyri ... | 2017 | 28424663 |
antimicrobial and antibiofilm activities of mta supplemented with bismuth lipophilic nanoparticles. | the objective of this work was to determine the antimicrobial and antibiofilm properties of mineral trioxide aggregate (mta) supplemented with bismuth lipophilic nanoparticles (bisbal nps). the antimicrobial activity of the composite mta-bisbal nps was determined by the disk diffusion assay, while antibiofilm activity was analyzed by fluorescence microscopy. the cytotoxicity of mta-bisbal nps was determined on human gingival fibroblasts by optical microscopy and crystal violet staining. mta-bisb ... | 2017 | 28420830 |
nitric oxide charged catheters as a potential strategy for prevention of hospital acquired infections. | catheter-associated hospital-acquired infections (hai's) are caused by biofilm-forming bacteria. using a novel approach, we generated anti-infective barrier on catheters by charging them with nitric oxide (no), a naturally-produced gas molecule. no is slowly released from the catheter upon contact with physiological fluids, and prevents bacterial colonization and biofilm formation onto catheter surfaces. | 2017 | 28410367 |
enterococcus faecalis bacteriocin entv inhibits hyphal morphogenesis, biofilm formation, and virulence of candida albicans. | enterococcus faecalis, a gram-positive bacterium, and candida albicans, a fungus, occupy overlapping niches as ubiquitous constituents of the gastrointestinal and oral microbiome. both species also are among the most important and problematic, opportunistic nosocomial pathogens. surprisingly, these two species antagonize each other's virulence in both nematode infection and in vitro biofilm models. we report here the identification of the e. faecalis bacteriocin, entv, produced from the entv (ef ... | 2017 | 28396417 |
[distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study]. | objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ... | 2017 | 28810329 |
efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces. | air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ... | 2017 | 28803189 |
antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study. | because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives for the treatment of infectious diseases, and are currently gaining more importance. we examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against some standard and clinical isolates of pseudomonas aeruginosa, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, methicillin susceptible/resistant s ... | 2017 | 28761777 |
antimicrobial susceptibility among gram-positive and gram-negative organisms collected from the latin american region between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial. | the in vitro activity of tigecycline and comparator agents was evaluated against gram-positive and gram-negative isolates collected in latin american centers between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) global surveillance study. | 2017 | 28701170 |
investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer infections. | skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management. | 2017 | 28689800 |
patterns of multi-drug resistant bacteria at first culture from patients admitted to a third level university hospital in calabria from 2011 to 2014: implications for empirical therapy and infection control. | surveillance of antimicrobial drug resistance is fundamental to guide empirical treatment. however, the european antimicrobial resistance surveillance network provides a general picture, which might not be applicable to clinical settings that are excluded from this survey. we evaluated resistance patterns of eskape isolates over a four-year period in a third level university hospital in the province of catanzaro (southern italy). in this retrospective study, we evaluated the frequency of eskape ... | 2017 | 28603227 |
evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms. | scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus). | 2017 | 28587870 |
antimicrobial efficacy of triple antibiotic-eluting polymer nanofibers against multispecies biofilm. | the elimination of microbial flora in cases of immature permanent teeth with necrotic pulp is both key and a challenging goal for the long-term success of regenerative therapy. recent research has focused on the development of cell-friendly intracanal drug delivery systems. this in vitro study aimed to investigate the antimicrobial action of 3-dimensional (3d) tubular-shaped triple antibiotic-eluting nanofibrous constructs against a multispecies biofilm on human dentin. polydioxanone polymer sol ... | 2017 | 28778504 |
disulfide bonds: a key modification in bacterial extracytoplasmic proteins. | disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. recent advances clearly show that oral bacteria also have the abil ... | 2017 | 28797211 |
safety evaluation of enterocin producer enterococcus sp. strains isolated from traditional turkish cheeses. | the purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. enterococcus strains were isolated from 33 different cheeses. enterococcus faecium (6 strains) and enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16s rdna analyses. structural genes enta, entb, entp and entx were detected in some isolates. mu ... | 2017 | 28735309 |
isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india. | a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ... | 2017 | 28720951 |
inactivation of bacterial biofilms by visible light-activated unmodified zno nanorods. | various zinc oxide (zno) nanostructures are widely used for photocatalytic antibacterial applications. since zno possess a wide bandgap, it is believed that only uv light may efficiently assist bacterial inactivation and diverse crystal lattice modifications should be applied in order to narrow the bandgap for efficient visible light absorption. in this work we show that even unmodified zno nanorods grown by aqueous chemical growth technique were found to possess intrinsic defects which can be a ... | 2017 | 28628486 |
characterization of high exopolysaccharide-producing lactobacillus strains isolated from mustard pickles for potential probiotic applications. | the aim of this study was to characterize high exopolysaccharide (eps)-producing lactic acid bacteria (lab) isolated from mustard pickles in taiwan for potential probiotic applications. among 39 collected lab strains, four most productive eps-producing strains were selected for further analysis. comparative analyses of 16s rdna genes rpoa and phes sequences demonstrated that these strains were members of lactobacillus plantarum-group (lpg). ncd 2, nld 4, slc 13, and nld 16 showed survival rates ... | 2017 | 28617525 |
seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium. | seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ... | 2017 | 28733150 |
physicochemical characterization of water-soluble chitosan derivatives with singlet oxygen quenching and antibacterial capabilities. | new water-soluble chitosan derivatives (wsch) were obtained by maillard reaction (mr) between glucosamine (ga) with both low and medium molecular weight chitosans (ch). the wsch showed larger solubility than the respective ch, while their deacetylation degree (dd) decreased by approximately 12%. infrared spectroscopy experiments of wsch confirmed the formation of imine bonds after mr with intensified pyranose structure, and sugar molecules as polymer branches. however, a 6-times reduction of the ... | 2017 | 28396271 |