Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| molecular characterization of extended-spectrum-cephalosporin-resistant enterobacteriaceae from wild kelp gulls in south america. | extended-spectrum-cephalosporin-resistant enterobacteriaceae are a public health concern due to limited treatment options. here, we report on the occurrence and the molecular characteristics of extended-spectrum-cephalosporin-resistant enterobacteriaceae recovered from wild birds (kelp gulls). our results revealed kelp gulls as a reservoir of various extended-spectrum cephalosporinase genes associated with different genetic platforms. in addition, we report for the first time the presence of a k ... | 2016 | 27572403 |
| emergence of host-adapted salmonella enteritidis through rapid evolution in an immunocompromised host. | host adaptation is a key factor contributing to the emergence of new bacterial, viral and parasitic pathogens. many pathogens are considered promiscuous because they cause disease across a range of host species, while others are host-adapted, infecting particular hosts(1). host adaptation can potentially progress to host restriction, where the pathogen is strictly limited to a single host species and is frequently associated with more severe symptoms. host-adapted and host-restricted bacterial c ... | 2016 | 27572160 |
| a feed-forward loop between sroc and mgrr small rnas modulates the expression of eptb and the susceptibility to polymyxin b in salmonella typhimurium. | base-pairing small rnas (srnas) regulate gene expression commonly by direct interaction with cognate mrnas. nevertheless, recent studies have expanded this knowledge with the discovery of the rna 'sponges' which are able to interact and repress the functions of classical base-pairing srnas. in this work, we present evidence indicating that the sponge rna sroc from salmonella enterica serovar typhimurium base pairs with the mgrr srna, thereby antagonizing its regulatory effects on both gene expre ... | 2016 | 27571709 |
| lose to win: mart pseudogenization in salmonella enterica serovar typhi contributed to the surv-dependent survival to h2o2, and inside human macrophage-like cells. | the difference in host range between salmonella enterica serovar typhimurium (s. typhimurium) and salmonella enterica serovar typhi (s. typhi) can be partially attributed to the gain of functions, to the loss of functions (i.e. pseudogenization), or to a combination of both processes. as previously reported, the loss of functions by pseudogenization may play a role in bacterial evolution, especially in host-restricted pathogens such as s. typhi. the mart-fidl operon, located at the spi-3, encode ... | 2016 | 27567490 |
| biosynthesis of salmonella enterica [nife]-hydrogenase-5: probing the roles of system-specific accessory proteins. | a subset of bacterial [nife]-hydrogenases have been shown to be capable of activating dihydrogen-catalysis under aerobic conditions; however, it remains relatively unclear how the assembly and activation of these enzymes is carried out in the presence of air. acquiring this knowledge is important if a generic method for achieving production of o2-resistant [nife]-hydrogenases within heterologous hosts is to be developed. salmonella enterica serovar typhimurium synthesizes the [nife]-hydrogenase- ... | 2016 | 27566174 |
| bioactive extracts from berry byproducts on the pathogenicity of salmonella typhimurium. | the aim of this study was to evaluate the phenotypic and genotypic properties of salmonella enterica serovar typhimurium (st) in the presence of lethal and sublethal concentrations (slc2log) of blackberry (rubus fruticosus) and blueberry (vaccinium corymbosum) pomace extracts. antimicrobial susceptibility, physicochemical properties, motility, biofilm formation ability, virulence gene expression patterns, and the ability of st to colonize in chick cecum were evaluated in the presence of these bi ... | 2016 | 27565525 |
| novel insights from molecular docking of sdia from salmonella enteritidis and escherichia coli with quorum sensing and quorum quenching molecules. | quorum sensing is a cell-to-cell communication mechanism leading to differential gene expression in response to high population density. the autoinducer-1 (ai-1) type quorum sensing system is incomplete in escherichia coli and salmonella due to the lack of the ai-1 synthase (luxi homolog) responsible for acyl homoserine lactone (ahl) synthesis. however, these bacteria encode the ahl receptor sdia (a luxr homolog) leading to gene regulation in response to ai-1 produced by other bacteria. this stu ... | 2016 | 27565088 |
| the impact of 18 ancestral and horizontally-acquired regulatory proteins upon the transcriptome and srna landscape of salmonella enterica serovar typhimurium. | we know a great deal about the genes used by the model pathogen salmonella enterica serovar typhimurium to cause disease, but less about global gene regulation. new tools for studying transcripts at the single nucleotide level now offer an unparalleled opportunity to understand the bacterial transcriptome, and expression of the small rnas (srna) and coding genes responsible for the establishment of infection. here, we define the transcriptomes of 18 mutants lacking virulence-related global regul ... | 2016 | 27564394 |
| the occurrence of salmonella spp. in duck eggs on sale at retail or from catering in england. | since 2010, human salmonellosis outbreaks in the uk have been detected as associated with the consumption of duck eggs. little data are available on the rate of occurrence of salmonella in duck eggs. the aim of this study was to investigate the occurrence of salmonella spp. in duck eggs on sale and from catering in england during 2011, particularly those from small-scale production. all samples were collected independently of human salmonellosis outbreak investigations. composite samples of 6-10 ... | 2016 | 27561893 |
| prophylactic bacteriophage administration more effective than post-infection administration in reducing salmonella enterica serovar enteritidis shedding in quail. | infections caused by salmonella bacteria, often through poultry products, are a serious public health issue. because of drawbacks associated with antibiotic prophylaxis, alternative treatments are sought. bacterial viruses (bacteriophages) may provide an effective alternative, but concerns remain with respect to bacteriophage stability and effectiveness. to this end, we assessed the stability of a novel bacteriophage isolated from poultry excreta, siphovirus pse, and its effectiveness in reducin ... | 2016 | 27555842 |
| what's in a name? species-wide whole-genome sequencing resolves invasive and noninvasive lineages of salmonella enterica serotype paratyphi b. | for 100 years, it has been obvious that salmonella enterica strains sharing the serotype with the formula 1,4,[5],12:b:1,2-now known as paratyphi b-can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. despite considerable predicted diversity between strains carrying the common paratyphi b serotype, there remain few methods that subdivide the group into groups that are congruent with their disease phenotypes. paratyphi b therefore represents one of the can ... | 2016 | 27555304 |
| influence of water activity on inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in peanut butter by microwave heating. | this study evaluated the efficacy of a 915 mhz microwave with 3 different electric power levels to inactivate three pathogens in peanut butter with different aw. peanut butter inoculated with escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes (0.3, 0.4, and 0.5 aw) were treated with a 915 mhz microwave with 2, 4, and 6 kw for up to 5 min. six kw 915 mhz microwave treatment for 5 min reduced these three pathogens by 1.97 to >5.17 log cfu/g. four kw 915 mh ... | 2016 | 27554151 |
| do bacterial cell numbers follow a theoretical poisson distribution? comparison of experimentally obtained numbers of single cells with random number generation via computer simulation. | we investigated a bacterial sample preparation procedure for single-cell studies. in the present study, we examined whether single bacterial cells obtained via 10-fold dilution followed a theoretical poisson distribution. four serotypes of salmonella enterica, three serotypes of enterohaemorrhagic escherichia coli and one serotype of listeria monocytogenes were used as sample bacteria. an inoculum of each serotype was prepared via a 10-fold dilution series to obtain bacterial cell counts with me ... | 2016 | 27554145 |
| molecular characterization of salmonella enterica serovar aberdeen negative for h2s production in china. | salmonella enterica infections continue to be a significant burden on public health worldwide. the ability of s. enterica to produce hydrogen sulfide (h2s) is an important phenotypic characteristic used to screen and identify salmonella with selective medium; however, h2s-negative salmonella have recently emerged. in this study, the h2s phenotype of salmonella isolates was confirmed, and the selected isolates were subjected to antimicrobial susceptibility testing and molecular identification by ... | 2016 | 27552230 |
| modulation of the substitution pattern of 5-aryl-2-aminoimidazoles allows fine-tuning of their antibiofilm activity spectrum and toxicity. | we previously synthesized several series of compounds, based on the 5-aryl-2-aminoimidazole scaffold, that showed activity preventing the formation of salmonella enterica serovar typhimurium and pseudomonas aeruginosa biofilms. here, we further studied the activity spectrum of a number of the most active n1- and 2n-substituted 5-aryl-2-aminoimidazoles against a broad panel of biofilms formed by monospecies and mixed species of bacteria and fungi. an n1-substituted compound showed very strong act ... | 2016 | 27550355 |
| identification of bacterial contaminants in polyherbal medicines used for the treatment of tuberculosis in amatole district of the eastern cape province, south africa, using rapid 16s rrna technique. | polyherbal medicines are used for the treatment of many diseases in many african and asian communities. with the increasing use of these remedies, several investigations have shown that they are associated with a broad variety of residues and contaminants. this study investigates the presence of bacteria in the polyherbal medicines used for the treatment of tuberculosis (tb) in the eastern cape province of south africa. | 2016 | 27549141 |
| distinct salmonella enteritidis lineages associated with enterocolitis in high-income settings and invasive disease in low-income settings. | an epidemiological paradox surrounds salmonella enterica serovar enteritidis. in high-income settings, it has been responsible for an epidemic of poultry-associated, self-limiting enterocolitis, whereas in sub-saharan africa it is a major cause of invasive nontyphoidal salmonella disease, associated with high case fatality. by whole-genome sequence analysis of 675 isolates of s. enteritidis from 45 countries, we show the existence of a global epidemic clade and two new clades of s. enteritidis t ... | 2016 | 27548315 |
| evaluation of a selected lactic acid bacteria-based probiotic on salmonella enterica serovar enteritidis colonization and intestinal permeability in broiler chickens. | two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (floramax-b11(®)) against salmonella enterica serovar enteritidis intestinal colonization and intestinal permeability in broiler chickens. experiment 1 consisted of two independent trials. in each trial, day-old broiler chicks were assigned to one of two groups: control + s. enteritidis or probiotic + s. enteritidis. at 72 h post-s. enteritidis challenge, haematology and caecal content were evaluated ... | 2017 | 27545145 |
| the antibacterial activity of coriolus versicolor methanol extract and its effect on ultrastructural changes of staphylococcus aureus and salmonella enteritidis. | the antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete coriolus versicolor was examined. the minimum inhibitory concentration (mic) values against various bacteria ranged from 0.625 to 20 mg ml(-1). c. versicolor expressed bactericidal activity against both gram-positive and gram-negative bacteria. the growth curves of staphylococcus aureus and salmonella enterica serovar enteritidis, measured at 630 nm, and confirmed with macrodilution met ... | 2016 | 27540376 |
| single molecule super-resolution imaging of proteins in living salmonella enterica using self-labelling enzymes. | the investigation of the subcellular localization, dynamics and interaction of proteins and protein complexes in prokaryotes is complicated by the small size of the cells. super-resolution microscopy (srm) comprise various new techniques that allow light microscopy with a resolution that can be up to ten-fold higher than conventional light microscopy. application of srm techniques to living prokaryotes demands the introduction of suitable fluorescent probes, usually by fusion of proteins of inte ... | 2016 | 27534893 |
| using a human challenge model of infection to measure vaccine efficacy: a randomised, controlled trial comparing the typhoid vaccines m01zh09 with placebo and ty21a. | typhoid persists as a major cause of global morbidity. while several licensed vaccines to prevent typhoid are available, they are of only moderate efficacy and unsuitable for use in children less than two years of age. development of new efficacious vaccines is complicated by the human host-restriction of salmonella enterica serovar typhi (s. typhi) and lack of clear correlates of protection. in this study, we aimed to evaluate the protective efficacy of a single dose of the oral vaccine candida ... | 2016 | 27533046 |
| outer membrane vesicle biosynthesis in salmonella: is there more to gram-negative bacteria? | recent research has focused on the biological role of outer membrane vesicles (omvs), which are derived from the outer membranes (oms) of gram-negative bacteria, and their potential exploitation as therapeutics. omvs have been characterized in many ways and functions. until recently, research focused on hypothetical and empirical models that addressed the molecular mechanisms of omv biogenesis, such as vesicles bulging from the om in various ways. the recently reported study by elhenawy et al. ( ... | 2016 | 27531914 |
| draft genome sequence of blatem-1-mediated cephalosporin-resistant salmonella enterica serovar typhi from bloodstream infection. | enteric fever is a major cause of concern in developing countries across the globe. the primary choice of antibiotics remains fluoroquinolones, followed by cephalosporins. resistance to third-generation cephalosporins is rarely reported in salmonella enterica serovar typhi. this study reports the whole genome sequence of an s. typhi isolate resistant to cefixime [minimum inhibitory concentration (mic)=512μg/ml] by microbroth dilution. interestingly, the isolate was negative for the cephalosporin ... | 2016 | 27530999 |
| analysis of plasmid-mediated quinolone and oxyimino-cephalosporin resistance mechanisms in uruguayan salmonella enterica isolates from 2011-2013. | this study characterised the mechanisms of fluoroquinolone and oxyimino-cephalosporin resistance in human salmonella enterica isolates in uruguay. salmonella enterica isolates were collected from 2011-2013 and were selected based on non-susceptibility to ciprofloxacin and/or oxyimino-cephalosporins. the disk diffusion assay was performed for various antibiotics, and the ciprofloxacin minimum inhibitory concentration (mic) was determined following clsi guidelines. genetic relatedness was determin ... | 2016 | 27530862 |
| simultaneous occurrence of salmonella enterica, campylobacter spp. and yersinia enterocolitica along the pork production chain from farm to meat processing in five conventional fattening pig herds in lower saxony. | the objectives of this study were to gather data on the occurrence of salmonella (s.) enterica, campylobacter spp. and yersinia (y.) enterocolitica along the pork production chain and to further analyze detected salmonella isolates by additionally applying mlva (multiple-locus variable-number tandem repeat analysis). in total, 336 samples were collected at primary production, slaughter and meat processing from five conventional fattening pig farms and one common slaughterhouse. at farm level, s. ... | 2016 | 27529991 |
| antifungal and antibacterial activities of petroselinum crispum essential oil. | parsley [petroselinum crispum (mill.) fuss] is regarded as an aromatic, culinary, and medicinal plant and is used in the cosmetic, food, and pharmaceutical industries. however, few studies with conflicting results have been conducted on the antimicrobial activity of parsley essential oil. in addition, there have been no reports of essential oil obtained from parsley aerial parts, except seeds, as an alternative natural antimicrobial agent. also, microorganism resistance is still a challenge for ... | 2016 | 27525894 |
| [investigation of antitumorigenic effects of food-borne non-pathogenic and pathogenic salmonella enterica strains on mef, du145 and hela cell lines]. | basic applications in cancer therapy may fail to eradicate cancer cells completely, they can show toxic affects to healthy cells and development of resistance to antitumor agents may increase tendency to metastasis. bacterial therapies have the advantage of specific targetting of tumors by selective toxicity, responsiveness to external signals, self-propelling capacity, and the sense of microenvironment. the most interest on the bacterial cancer therapy is about salmonella spp. with a special em ... | 2016 | 27525394 |
| whole-genome sequencing analysis of salmonella enterica serovar enteritidis isolates in chile provides insights into possible transmission between gulls, poultry, and humans. | salmonella enterica subsp. enterica serotype enteritidis is a major cause of human salmonellosis worldwide; however, little is known about the genetic relationships between s enteritidis clinical strains and s enteritidis strains from other sources in chile. we compared the whole genomes of 30 s enteritidis strains isolated from gulls, domestic chicken eggs, and humans in chile, to investigate their phylogenetic relationships and to establish their relatedness to international strains. core geno ... | 2016 | 27520817 |
| extended-spectrum β-lactamase-producing salmonella enterica serovar infantis from humans in italy. | 2016 | 27516228 | |
| emerging enteric fever due to switching biotype of salmonella (paratyphi a) in eastern odisha. | typhoid fever is classically caused by salmonella enterica serotype typhi.recently the frequency of isolation of s. paratyphi a (spa) has been increased in comparison to s. typhi in indian scenario. | 2017 | 27510670 |
| mapping b-cell responses to salmonella enterica serovars typhimurium and enteritidis in chickens for the discrimination of infected from vaccinated animals. | serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. however, the compatibility of these strategies is limited by a lack of assays capable of differentiating infected from vaccinated animals (diva tests) for established killed or attenuated vaccines. here, we used next generation phage-display (ngpd) and a 2-proportion z score analysis to identify peptides that were preferentially bound by igy from chickens infected wit ... | 2016 | 27510219 |
| antimicrobial resistance in zoonotic nontyphoidal salmonella: an alarming trend? | zoonotic bacteria of the genus salmonella have acquired various antimicrobial resistance properties over the years. the corresponding resistance genes are commonly located on plasmids, transposons, gene cassettes, or variants of the salmonella genomic islands sgi1 and sgi2. human infections by nontyphoidal salmonella isolates mainly result from ingestion of contaminated food. the two predominantly found salmonella enterica subsp. enterica serovars in the usa and in europe are s. enteritidis and ... | 2016 | 27506509 |
| salmonella typhimurium utilizes a t6ss-mediated antibacterial weapon to establish in the host gut. | the mammalian gastrointestinal tract is colonized by a high-density polymicrobial community where bacteria compete for niches and resources. one key competition strategy includes cell contact-dependent mechanisms of interbacterial antagonism, such as the type vi secretion system (t6ss), a multiprotein needle-like apparatus that injects effector proteins into prokaryotic and/or eukaryotic target cells. however, the contribution of t6ss antibacterial activity during pathogen invasion of the gut ha ... | 2016 | 27503894 |
| a profile-based method for identifying functional divergence of orthologous genes in bacterial genomes. | next generation sequencing technologies have provided us with a wealth of information on genetic variation, but predicting the functional significance of this variation is a difficult task. while many comparative genomics studies have focused on gene flux and large scale changes, relatively little attention has been paid to quantifying the effects of single nucleotide polymorphisms and indels on protein function, particularly in bacterial genomics. | 2016 | 27503221 |
| measurement of the accurate mass of a 50 mda infectious virus. | bacteriophage p22 is believed to contain a total of 521 copies of 9 different proteins and a 41,724 base pair genome. despite its enormous size and complexity, phage p22 can be electrosprayed, and it remains intact in ultra-high vacuum where its molar mass distribution has been measured. | 2016 | 27501430 |
| efficacy of tumor-targeting salmonella a1-r on a melanoma patient-derived orthotopic xenograft (pdox) nude-mouse model. | tumor-targeting salmonella enterica serovar typhimurium a1-r (salmonella a1-r) had strong efficacy on a melanoma patient-derived orthotopic xenograft (pdox) nude-mouse model. gfp-expressing salmonella a1-r highly and selectively colonized the pdox melanoma and significantly suppressed tumor growth (p = 0.021). the combination of salmonella a1-r and cisplatinum (cddp), both at low-dose, also significantly suppressed the growth of the melanoma pdox (p = 0.001). salmonella a1-r has future clinical ... | 2016 | 27500926 |
| effects of salmonella infection on hepatic damage following acute liver injury in rats. | acute liver injury is a common clinical disorder associated with intestinal barrier injury and disturbance of intestinal microbiota. probiotic supplementation has been reported to reduce liver injury; however, it is unclear whether enteropathogen infection exacerbates liver injury. the purpose of this study was to address this unanswered question using a rat model. | 2016 | 27498580 |
| the molecular chaperone dnak is a source of mutational robustness. | molecular chaperones, also known as heat-shock proteins, refold misfolded proteins and help other proteins reach their native conformation. thanks to these abilities, some chaperones, such as the hsp90 protein or the chaperonin groel, can buffer the deleterious phenotypic effects of mutations that alter protein structure and function. hsp70 chaperones use a chaperoning mechanism different from that of hsp90 and groel, and it is not known whether they can also buffer mutations. here, we show that ... | 2016 | 27497316 |
| isolation and characterization of antimicrobial-resistant nontyphoidal salmonella enterica serovars from imported food products. | the objective of this study was to determine antimicrobial resistance and elucidate the resistance mechanism in nontyphoidal salmonella enterica serovars isolated from food products imported into the united states from 2011 to 2013. food products contaminated with antimicrobial-resistant nontyphoidal s. enterica were mainly imported from taiwan, indonesia, vietnam, and china. pcr, dna sequencing, and plasmid analyses were used to characterize antimicrobial resistance determinants. twentythree of ... | 2016 | 27497122 |
| prevalence and characterization of salmonella enterica and salmonella bacteriophages recovered from beef cattle feedlots in south texas. | asymptomatic salmonella carriage in beef cattle is a food safety concern, and the beef feedlot environment may function as a reservoir of this pathogen. the goal of this study was to identify and isolate salmonella and salmonella bacteriophages from beef cattle feedlot environments in order to better understand the microbial ecology of salmonella and identify phages that might be useful as anti-salmonella beef safety interventions. three feedlots in south texas were visited, and 27 distinct samp ... | 2016 | 27497120 |
| feeding a high dosage of zinc oxide affects suppressor of cytokine gene expression in salmonella typhimurium infected piglets. | suppressor of cytokine signaling (socs) proteins play an important role in the regulation of the immune response by inhibiting cytokines. here we investigated the effects of zinc oxide fed at three different dosages (lzn=57ppm, mzn=167ppm, hzn=2425ppm) to weaned piglets that were or were not orally infected with salmonella enterica serovar typhimurium dt 104. we detected higher expression of socs3 six days after weaning for all analyzed piglets, regardless of the infection or the zinc feeding, s ... | 2016 | 27496737 |
| the river ruhr - an urban river under particular interest for recreational use and as a raw water source for drinking water: the collaborative research project "safe ruhr" - microbiological aspects. | along the intense industrialization of the ruhr valley (germany), the river ruhr became increasingly polluted. over time, using it for recreational purposes became a serious health hazard and bathing was banned due to chemical and microbiological risks. the purpose of the collaborative project "safe ruhr" was to verify the current status and to provide a scientific basis for lifting the bathing ban. as the river also provides a raw water source for drinking water production, it was investigated ... | 2016 | 27495908 |
| recovery estimation of dried foodborne pathogens is directly related to rehydration kinetics. | drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. the aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary rehydration. rehydration of dried bacteria is a critical step already studied in starter culture but not for different kinetics and not for pathogens. in the present study, the influence of rehydration kinetics was investigated for three fo ... | 2016 | 27494169 |
| detection of quinolone resistance in salmonella typhimurium pig isolates determined by gyra gene mutation using pcr- and sequence-based techniques within the gyra gene. | the emergence of reduced susceptibility to fluoroquinolones among salmonella enterica serotype typhimurium isolates leading to clinical failure of treatment poses a great therapeutic challenge. | 2016 | 27494067 |
| effect of the motb(d33n) mutation on stator assembly and rotation of the proton-driven bacterial flagellar motor. | the bacterial flagellar motor generates torque by converting the energy of proton translocation through the transmembrane proton channel of the stator complex formed by mota and motb. the mota/b complex is thought to be anchored to the peptidoglycan (pg) layer through the pg-binding domain of motb to act as the stator. the stator units dynamically associate with and dissociate from the motor during flagellar motor rotation, and an electrostatic interaction between mota and a rotor protein flig i ... | 2014 | 27493496 |
| rpoe promotes invasion and intracellular survival by regulating spi-1 and spi-2 in salmonella enterica serovar typhi. | to demonstrate the role of rpoe during the later stage of hyperosmotic stress in salmonella. | 2016 | 27492279 |
| cadmium resistance and uptake by bacterium, salmonella enterica 43c, isolated from industrial effluent. | cadmium resistant bacterium, isolated from industrial wastewater, was characterized as salmonella enterica 43c on the basis of biochemical and 16s rrna ribotyping. it is first ever reported s. enterica 43c bared extreme resistance against heavy metal consortia in order of pb(2+)>cd(2+)>as(3+)>zn(2+)>cr(6+)>cu(2+)>hg(2+). cd(2+) stress altered growth pattern of the bacterium in time dependent manner. it could remove nearly 57 % cd(2+) from the medium over a period of 8 days. kinetic and thermodyn ... | 2016 | 27491862 |
| protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivated salmonella gallinarum vaccine candidate. | salmonella enterica serovar gallinarum (sg) ghost vaccine candidate was recently constructed. in this study, we evaluated various prime-boost vaccination strategies using the candidate strain to optimize immunity and protection efficacy against fowl typhoid. | 2016 | 27489805 |
| characterization and evaluation of a salmonella enterica serotype senftenberg mutant created by deletion of virulence-related genes for use as a live attenuated vaccine. | natural infections of chickens with salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg) are characterized by low-level intestinal invasiveness and insignificant production of antibodies. in this study, we investigated the potential effects of lon and cpxr gene deletions on the invasiveness of s senftenberg into the intestinal epithelium of chickens and its ability to induce an immune response, conferring protection against s senftenberg infection. with the allelic exchange m ... | 2016 | 27489135 |
| exploring the diversity and antimicrobial potential of marine actinobacteria from the comau fjord in northern patagonia, chile. | bioprospecting natural products in marine bacteria from fjord environments are attractive due to their unique geographical features. although, actinobacteria are well known for producing a myriad of bioactive compounds, investigations regarding fjord-derived marine actinobacteria are scarce. in this study, the diversity and biotechnological potential of actinobacteria isolated from marine sediments within the comau fjord, in northern chilean patagonia, were assessed by culture-based approaches. ... | 2016 | 27486455 |
| intracellular salmonella induces aggrephagy of host endomembranes in persistent infections. | xenophagy has been studied in epithelial cells infected with salmonella enterica serovar typhimurium (s. typhimurium). distinct autophagy receptors target this pathogen to degradation after interacting with ubiquitin on the surface of cytosolic bacteria, and the phagophore- and autophagosome-associated protein map1lc3/lc3. glycans exposed in damaged phagosomal membranes and diacylglycerol accumulation in the phagosomal membrane also trigger s. typhimurium xenophagy. how these responses control i ... | 2016 | 27485662 |
| quality attributes and microbial survival on whole cantaloupes with antimicrobial coatings containing chitosan, lauric arginate, cinnamon oil and ethylenediaminetetraacetic acid. | cantaloupes are susceptible to microbiological contamination in pre- or postharvest environments. novel intervention strategies, such as antimicrobial coatings, are needed to improve the microbiological safety of cantaloupes. the objective of this study was to prepare whole cantaloupes coated with mixtures containing chitosan, lauric arginate (lae), cinnamon oil (co), and ethylenediaminetetraacetic acid (edta) and determine survival characteristics of inoculated foodborne pathogens during storag ... | 2016 | 27484251 |
| advances in diagnosis, treatment, and prevention of invasive salmonella infections. | typhoidal and nontyphoidal salmonella enterica serotypes are among the most common bacterial causes of acute febrile illnesses in the developing world. in this review, we discuss new advances in understanding of the burden, diagnostic approaches, treatment and vaccines for invasive salmonella infections. | 2016 | 27479027 |
| alterations in ribosomal protein l19 that decrease the fidelity of translation. | ribosomal protein l19 is an essential ribosomal protein and is a component of bridge b8, one of the protein-rna bridges linking the large and small ribosomal subunits. bridge b8 also contributes to the accuracy of translation by affecting gtpase activation by ribosome-bound aminoacyl trna-ef-tu•gtp ternary complexes. previous work has identified a limited number of accuracy-altering alterations in protein l19 of salmonella enterica and thermus thermophilus. here, we have targeted the escherichia ... | 2017 | 27477481 |
| heterologous pseudomonas aeruginosa o-antigen delivery using a salmonella enterica serovar typhimurium weca mutant strain. | there is a broad interest in adapting live vaccine strains (lvs) for use as recombinant vaccines that can deliver heterologous antigens. the salmonella enterica serovar typhimurium sl1344 δweca lvs contains a mutation in weca that abrogates production of enterobacterial common antigen. this δweca strain is attenuated in vivo, persistently colonizes the host, and protects against both wild type and cross-salmonella serovar lethal challenge in a murine model of salmonellosis. given these character ... | 2016 | 27476047 |
| the effectors and sensory sites of formaldehyde-responsive regulator frmr and metal-sensing variant. | the duf156 family of dna-binding transcriptional regulators includes metal sensors that respond to cobalt and/or nickel (rcnr, inrs) or copper (csor) plus cstr, which responds to persulfide, and formaldehyde-responsive frmr. unexpectedly, the allosteric mechanism of frmr from salmonella enterica serovar typhimurium is triggered by metals in vitro, and variant frmr(e64h) gains responsiveness to zn(ii) and cobalt in vivo here we establish that the allosteric mechanism of frmr is triggered directly ... | 2016 | 27474740 |
| evaluation of protective immune response against fowl typhoid in chickens vaccinated with the attenuated strain salmonella gallinarum δcobsδcbia. | salmonella enterica serovar gallinarum biovar gallinarum (sg) causes fowl typhoid in chickens, a septicemic infection which results in high mortality rates. this disease causes high economic impact to the poultry industry worldwide because of the mortality or elimination of positive flocks to control bacterial dissemination. live vaccines are used in the fields, however the characterization of immune mechanisms important for protection are being studied to improve the efficacy of vaccination sch ... | 2016 | 27473999 |
| the salmonella effector sopb prevents ros-induced apoptosis of epithelial cells by retarding traf6 recruitment to mitochondria. | microbial pathogens enter host cells by injecting effector proteins of the type iii secretion system (t3ss), which facilitate pathogen translocation across the host cell membrane. these effector proteins exert their effects by modulating a variety of host innate immune responses, thereby facilitating bacterial replication and systemic infection. salmonella enterica serovar typhimurium (s.typhimurium) is a clinically important pathogen that causes food poisoning and gastroenteritis. the sopb effe ... | 2016 | 27473656 |
| identification of salmonella enterica serovar kentucky genes involved in attachment to chicken skin. | regardless of sanitation practices implemented to reduce salmonella prevalence in poultry processing plants, the problem continues to be an issue. to gain an understanding of the attachment mechanism of salmonella to broiler skin, a bioluminescent-based mutant screening assay was used. a random mutant library of a field-isolated bioluminescent strain of salmonella enterica serovar kentucky was constructed. mutants' attachment to chicken skin was assessed in 96-well plates containing uniform 6 mm ... | 2016 | 27473153 |
| chicken-specific kinome array reveals that salmonella enterica serovar enteritidis modulates host immune signaling pathways in the cecum to establish a persistence infection. | non-typhoidal salmonella enterica induces an early, short-lived pro-inflammatory response in chickens that is asymptomatic of clinical disease and results in a persistent colonization of the gastrointestinal (gi) tract that transmits infections to naïve hosts via fecal shedding of bacteria. the underlying mechanisms that control this persistent colonization of the ceca of chickens by salmonella are only beginning to be elucidated. we hypothesize that alteration of host signaling pathways mediate ... | 2016 | 27472318 |
| management of osteomyelitis caused by salmonella enterica subsp. houtenae in a taylor's cantil (agkistrodon bilineatus taylori) using amikacin delivered via osmotic pump. | an adult female taylor's cantil (agkistrodon bilineatus taylori) presented with marked spinal and mandibular osteomyelitis that cultured positive for salmonella enterica subsp. houtenae, serovar iv 43:z4,z32:-. progression of osteomyelitis was arrested by treatment using amikacin (0.026 mg/kg per hour) delivered via subcutaneous osmotic pump for 10 mo, replacing the pump every 4 wk. no adverse effects on renal function were appreciated throughout the course of therapy. amikacin therapy was disco ... | 2016 | 27468053 |
| salmonella and the inflammasome: battle for intracellular dominance. | inflammasomes are macromolecular cytoplasmic complexes that act as signaling platforms for the activation of inflammatory caspases. their activation triggers the processing and secretion of the pro-inflammatory cytokines il-1β and il-18, as well as the induction of a specialized form of inflammatory cell death termed pyroptosis. here, we review the mechanisms of inflammasome activation triggered by the intracellular pathogen salmonella enterica serovar typhimurium. we highlight the different inf ... | 2017 | 27460804 |
| dispersal of human and plant pathogens biofilms via nitric oxide donors at 4 °c. | recent studies suggest that nitric oxide donors capable of manipulating nitric oxide-mediated signaling in bacteria could induce dispersal of biofilms. encased in extracellular polymeric substances, human and plant pathogens within biofilms are significantly more resistant to sanitizers. this is particularly a problem in refrigerated environments where food is processed. in an exercise aimed to study the potential of nitric oxide donors as biofilm dispersal in refrigerated conditions, we compare ... | 2016 | 27457245 |
| an immuno-magnetic nanobead probe competitive assay for rapid detection of salmonella choleraesuis. | a competitive lateral flow assay for the rapid detection of salmonella choleraesuis was developed. immuno-magnetic nanobeads were produced by covalently coupling anti-salmonella choleraesuis antibody to magnetic nanobeads. these immuno-magnetic nanobeads were used as visually detected probes in the subsequent assay. compared with the traditional sandwich assay, which is used for detecting macro-molecules, this new method was developed based on the competitive relationship between s. choleraesuis ... | 2016 | 27455631 |
| a salmonella nanoparticle mimic overcomes multidrug resistance in tumours. | salmonella enterica serotype typhimurium is a food-borne pathogen that also selectively grows in tumours and functionally decreases p-glycoprotein (p-gp), a multidrug resistance transporter. here we report that the salmonella type iii secretion effector, sipa, is responsible for p-gp modulation through a pathway involving caspase-3. mimicking the ability of salmonella to reverse multidrug resistance, we constructed a gold nanoparticle system packaged with a sipa corona, and found this bacterial ... | 2016 | 27452236 |
| encapsulation of multiple cargo proteins within recombinant eut nanocompartments. | spatial organization via encapsulation of enzymes within recombinant nanocompartments may increase efficiency in multienzyme cascades. previously, we reported the encapsulation of single cargo proteins within nanocompartments in the heterologous host escherichia coli. this was achieved by coexpression of the salmonella enterica lt2 ethanolamine utilization bacterial microcompartment shell proteins euts or eutsmnlk, with a signal sequence eutc1-19 cargo protein fusion. optimization of this system ... | 2016 | 27450681 |
| the efficacy of an inhibin dna vaccine delivered by attenuated salmonella choleraesuis on follicular development and ovulation responses in crossbred buffaloes. | the aim of this study was to evaluate the efficacy of an inhibin dna vaccine delivered by attenuated salmonella choleraesuis on follicular development and ovulation responses in crossbred buffaloes. a total of 158 crossbred buffaloes divided into four groups and were intramuscularly injected with 1×10(10) (t1, n=41), 1×10(9) (t2, n=37), 1×10(8) (t3, n=37) or 0 (c, n=43) cfu/ml bacteria delivered inhibin vaccine in 10ml pbs on day 0 and 14, respectively. all animals were administered with 1000 iu ... | 2016 | 27449408 |
| breast abscess due to salmonella typhimurium in a patient with rheumatoid arthritis: a case report. | this is the first report of breast abscess due to salmonella enterica serotype typhimurium. staphylococcus aureus is known as the most common cause of breast abscess. salmonella spp. may occasionally form localized abscesses after dissemination to various organ systems following a bacteraemia. but breast abscess related to salmonella spp is a very rare complication. | 2016 | 27448659 |
| characterization of five podoviridae phages infecting citrobacter freundii. | citrobacter freundii causes opportunistic infections in humans and animals, which are becoming difficult to treat due to increased antibiotic resistance. the aim of this study was to explore phages as potential antimicrobial agents against this opportunistic pathogen. we isolated and characterized five new virulent phages, sh1, sh2, sh3, sh4, and sh5 from sewage samples in tunisia. morphological and genomic analyses revealed that the five c. freundii phages belong to the caudovirales order, podo ... | 2016 | 27446058 |
| the crystal structure of the c-terminal domain of the salmonella enterica pduo protein: an old fold with a new heme-binding mode. | the two-domain protein pduo, involved in 1,2-propanediol utilization in the pathogenic gram-negative bacterium salmonella enterica is an atp:cob(i)alamin adenosyltransferase, but this is a function of the n-terminal domain alone. the role of its c-terminal domain (pduoc) is, however, unknown. in this study, comparative growth assays with a set of salmonella mutant strains showed that this domain is necessary for effective in vivo catabolism of 1,2-propanediol. it was also shown that isolated, re ... | 2016 | 27446048 |
| draft genome sequences of salmonella enterica subsp. enterica serovars typhimurium and nottingham isolated from food products. | a quantitative real-time pcr (qpcr) designed to detect salmonella enterica subsp. enterica serovar enteritidis, targeting the sdf gene, generated positive results for s. enterica subsp. enterica serovar typhimurium (cfsan033950) and s. enterica subsp. enterica serovar nottingham (cfsan006803) isolated from food samples. both strains show pulsed-field gel electrophoresis (pfge) patterns distinct from those of s enteritidis. here, we report the genome sequences of these two strains. | 2016 | 27445384 |
| prevalence and molecular characterization of salmonella enterica isolates throughout an integrated broiler supply chain in china. | a total of 1145 samples were collected from chicken breeder farms, hatcheries, broiler farms, a slaughterhouse and retail refrigerated chicken stores in an integrated broiler supply chain in guangdong province, china, in 2013. one-hundred and two salmonella enterica strains were isolated and subjected to serotyping, antimicrobial susceptibility testing, virulence profile determination and molecular subtyping by pulsed field gel electrophoresis (pfge). the contamination rates in samples from bree ... | 2016 | 27443305 |
| prevalence, seasonal occurrence, and antimicrobial resistance of salmonella spp. isolates recovered from chicken carcasses sampled at major poultry processing plants of south korea. | the current study was conducted to assess salmonella spp. contamination in chicken carcasses produced at major poultry processing plants in south korea. in total, 120 chicken carcasses were collected through 12 individual trials (10 chickens per trial) from six poultry processing plants in the summer of 2014 and the winter of 2015. eighteen chicken samples (15%) were contaminated with salmonella, with a higher rate of contamination observed during summer (14 isolates, 11.7%) than during winter ( ... | 2016 | 27442349 |
| inactivation of salmonella enterica and listeria monocytogenes in cantaloupe puree by high hydrostatic pressure with/without added ascorbic acid. | the objective of this research was to evaluate and develop a method for inactivation of salmonella enterica and listeria monocytogenes in cantaloupe puree (cp) by high hydrostatic pressure (hhp). cantaloupe being the most netted varieties of melons presents a greater risk of pathogen transmission. freshly prepared cp with or without 0.1% ascorbic acid (aa) was inoculated with a bacterial cocktail composed of a three serotype mixture of s. enterica (s. poona, s. newport h1275 and s. stanley h0558 ... | 2016 | 27441819 |
| capture and concentration of viral and bacterial foodborne pathogens using apolipoprotein h. | the need for improved pathogen separation and concentration methods to reduce time-to-detection for foodborne pathogens is well recognized. apolipoprotein h (apoh) is an acute phase human plasma protein that has been previously shown to interact with viruses, lipopolysaccharides (lps) and bacterial proteins. the purpose of this study was to determine if apoh was capable of binding and efficiently capturing two representative human norovirus strains (gi.1 and gii.4), a cultivable surrogate, and f ... | 2016 | 27439140 |
| nutrient deprivation affects salmonella invasion and its interaction with the gastrointestinal microbiota. | salmonella enterica serovar typhimurium (s. typhimurium) is a foodborne enteric pathogen and a major cause of gastroenteritis in humans. it is known that molecules derived from the human fecal microbiota downregulate s. typhimurium virulence gene expression and induce a starvation-like response. in this study, s. typhimurium was cultured in minimal media to mimic starvation conditions such as that experienced by s. typhimurium in the human intestinal tract, and the pathogen's virulence in vitro ... | 2016 | 27437699 |
| contribution to virulence of a novel anti-inflammatory effector in salmonella enterica. | 2015 | 27437422 | |
| energy taxis toward host-derived nitrate supports a salmonella pathogenicity island 1-independent mechanism of invasion. | salmonella enterica serovar typhimurium can cross the epithelial barrier using either the invasion-associated type iii secretion system (t3ss-1) or a t3ss-1-independent mechanism that remains poorly characterized. here we show that flagellum-mediated motility supported a t3ss-1-independent pathway for entering ileal peyer's patches in the mouse model. flagellum-dependent invasion of peyer's patches required energy taxis toward nitrate, which was mediated by the methyl-accepting chemotaxis protei ... | 2016 | 27435462 |
| transcription factor decr (ybao) controls detoxification of l-cysteine in escherichia coli. | ybao is an uncharacterized asnc-family transcription factor of escherichia coli. in both salmonella enterica and pantoea ananatis, ybao homologues were identified to regulate the adjacent gene encoding cysteine desulfhydrase for detoxification of cysteine. using the genomic selex (systematic evolution of ligands by exponential enrichment) screening system, we identified the yhaom operon, located far from the ybao gene on the e. coli genome, as a single regulatory target of ybao. in both gel shif ... | 2016 | 27435271 |
| first case of lung abscess due to salmonella enterica serovar abony in an immunocompetent adult patient. | in healthy individuals, nontyphoidal salmonella species predominantly cause a self-limited form of gastroenteritis, while they infrequently invade or cause fatal disease. extraintestinal manifestations of nontyphoidal salmonella infections are not common and mainly occur among individuals with specific risk factors; among them, focal lung infection is a rare complication caused by nontyphoidal salmonella strains typically occurring in immunocompromised patients with prior lung disease. we descri ... | 2016 | 27429814 |
| duplex identification of staphylococcus aureus by aptamer and gold nanoparticles. | staphylococcus aureus is the top common pathogen causing infections and food poisoning. identification of s. aureus is crucial for the disease diagnosis and regulation of food hygiene. herein, we report an aptamer-aunps based method for duplex identification of s. aureus. using aunps as an indicator, sa23, an aptamer against s. aureus, can well identify its target from escherichia coli, listeria monocytogenes and pseudomonas aeruginosa. furthermore, we find citrate-coated aunps can strongly bind ... | 2016 | 27427591 |
| tuning the catalytic activity of subcellular nanoreactors. | bacterial microcompartments are naturally occurring subcellular organelles of bacteria and serve as a promising scaffold for the organization of heterologous biosynthetic pathways. a critical element in the design of custom biosynthetic organelles is quantitative control over the loading of heterologous enzymes to the interior of the organelles. we demonstrate that the loading of heterologous proteins to the 1,2-propanediol utilization microcompartment of salmonella enterica can be controlled us ... | 2016 | 27427532 |
| gold nanoparticle-dna aptamer conjugate-assisted delivery of antimicrobial peptide effectively eliminates intracellular salmonella enterica serovar typhimurium. | antimicrobial peptides (amps) are a promising new class of antibacterial compounds. however, their applications in the treatment of intracellular pathogenic bacteria have been limited by their in vivo instability and low penetrating ability into mammalian cells. here, we report that gold nanoparticles conjugated with dna aptamer (aunp-apt) efficiently delivered amps into mammalian living systems with enhanced stability of the amps. c-terminally hexahistidine-tagged a3-apo (a3-apo(his)) amps were ... | 2016 | 27424215 |
| regulated delayed synthesis of lipopolysaccharide and enterobacterial common antigen of salmonella typhimurium enhances immunogenicity and cross-protective efficacy against heterologous salmonella challenge. | lipopolysaccharide (lps) o-antigen and enterobacterial common antigen (eca) are two major polysaccharide structures on the surface of salmonella enterica serovar typhimurium. previous studies have demonstrated that regulated truncation of lps enhances the cross-reaction against conserved outer membrane proteins (omps) from enteric bacteria. we speculate that the regulation of both o-antigen and eca may enhance the induction of immune responses against conserved omps from enteric bacteria. in thi ... | 2016 | 27423383 |
| the enterobacterial genotoxins: cytolethal distending toxin and colibactin. | while the dna damage induced by ionizing radiation and by many chemical compounds and drugs is well characterized, the genotoxic insults inflicted by bacteria are only scarcely documented. however, accumulating evidence indicates that we are exposed to bacterial genotoxins. the prototypes of such bacterial genotoxins are the cytolethal distending toxins (cdts) produced by escherichia coli and salmonella enterica serovar typhi. cdts display the dnase structure fold and activity, and induce dna st ... | 2016 | 27419387 |
| tissue colonization and circulating t lymphocytes in laying hens upon oral challenge with salmonella enterica serovars. | evaluating the potential of salmonella serovars for tissue colonization and egg contamination in laying hens is critical due to widespread consumption of poultry and egg-containing products. the 2009 fda egg rule was implemented to target the eradication of salmonella enterica enteritidis (se) from layers; however, other salmonella serovars, such as heidelberg (sh) and typhimurium (st), have also been associated with poultry-related outbreaks. we conducted this study to see if serovars other tha ... | 2016 | 27418660 |
| interferon-inducible guanylate-binding proteins at the interface of cell-autonomous immunity and inflammasome activation. | guanylate-binding proteins (gbps) are essential components of cell-autonomous immunity. in response to ifn signaling, gbps are expressed in the cytoplasm of immune and nonimmune cells, where they unleash their antimicrobial activity toward intracellular bacteria, viruses, and parasites. recent studies have revealed that gbps are essential for mediating activation of the caspase-1 inflammasome in response to the gram-negative bacteria salmonella enterica serovar typhimurium, francisella novicida, ... | 2017 | 27418355 |
| metabolic parameters linked by phenotype microarray to acid resistance profiles of poultry-associated salmonella enterica. | phenotype microarrays were analyzed for 51 datasets derived from salmonella enterica. the top 4 serotypes associated with poultry products and one associated with turkey, respectively typhimurium, enteritidis, heidelberg, infantis and senftenberg, were represented. datasets were partitioned initially into two clusters based on ranking by values at ph 4.5 (pm10 a03). negative control wells were used to establish 90 respiratory units as the point differentiating acid resistance from sensitive stra ... | 2017 | 27418207 |
| complete genome sequence of salmonella enterica subsp. enterica serovar indiana c629, a carbapenem-resistant bacterium isolated from chicken carcass in china. | the carbapenem-resistant salmonella enterica subsp. enterica serovar indiana strain c629 was isolated from a chicken carcass collected from a slaughterhouse in qingdao, china. the complete genome sequence of c629 contains a circular 4,791,723-bp chromosome and a circular 210,106-bp plasmid. genes involved in carbapenem resistance of this bacterium were identified by whole-genome analysis. | 2016 | 27417837 |
| draft genome sequence of salmonella enterica subsp. enterica serovar widemarsh strain crjjgf_00058 (phylum gammaproteobacteria). | here, we report a 4.73 mbp draft genome sequence of salmonella enterica subsp. enterica serovar widemarsh strain crjjgf_00058, isolated from eggs in 2008. | 2016 | 27417830 |
| draft genome sequence of salmonella enterica subsp. enterica serovar lille strain crjjgf_000101 (phylum gammaproteobacteria). | here, we report a 4.98 mbp draft genome sequence of salmonella enterica subsp. enterica serovar lille strain crjjgf_000101, isolated from ground beef in 2007. | 2016 | 27417829 |
| in vitro activity of tigemonam against urinary tract pathogens. | tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serrada marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory c ... | 1989 | 27416146 |
| application of edible coating with starch and carvacrol in minimally processed pumpkin. | the present study evaluated the effect of an edible coating of cassava starch and carvacrol in minimally processed pumpkin (mpp). the minimal inhibitory concentration (mic) of carvacrol against escherichia coli, salmonella enterica serotype typhimurium, aeromonas hydrophila, and staphylococcus aureus was determined. the edible coating that contained carvacrol at the mic and 2 × mic was applied to mpp, and effects were evaluated with regard to the survival of experimentally inoculated bacteria an ... | 2016 | 27413224 |
| salmonella serogroup c: current status of vaccines and why they are needed. | nontyphoidal salmonella (nts; i.e., salmonella enterica organisms that do not cause typhoid or paratyphoid) are responsible for 94 million infections and 155,000 deaths worldwide annually, 86% of which are estimated to be foodborne. although more than 50 serogroups and 2,600 serovars have been described, not all salmonella serovars cause disease in humans and animals. efforts are being made to develop nts vaccines, with most approaches eliciting protection against serovars typhimurium and enteri ... | 2016 | 27413069 |
| animal models for salmonellosis: applications in vaccine research. | salmonellosis remains an important cause of human disease worldwide. while there are several licensed vaccines for salmonella enterica serovar typhi, these vaccines are generally ineffective against other salmonella serovars. vaccines that target paratyphoid and nontyphoidal salmonella serovars are very much in need. preclinical evaluation of candidate vaccines is highly dependent on the availability of appropriate scientific tools, particularly animal models. many different animal models exist ... | 2016 | 27413068 |
| bayesian inference of the evolution of a phenotype distribution on a phylogenetic tree. | the distribution of a phenotype on a phylogenetic tree is often a quantity of interest. many phenotypes have imperfect heritability, so that a measurement of the phenotype for an individual can be thought of as a single realization from the phenotype distribution of that individual. if all individuals in a phylogeny had the same phenotype distribution, measured phenotypes would be randomly distributed on the tree leaves. this is, however, often not the case, implying that the phenotype distribut ... | 2016 | 27412711 |
| a novel covalent approach to bio-conjugate silver coated single walled carbon nanotubes with antimicrobial peptide. | due to increasing antibiotic resistance, the use of silver coated single walled carbon nanotubes (swcnts-ag) and antimicrobial peptides (aps) is becoming popular due to their antimicrobial properties against a wide range of pathogens. however, stability against various conditions and toxicity in human cells are some of the major drawbacks of aps and swcnts-ag, respectively. therefore, we hypothesized that aps-functionalized swcnts-ag could act synergistically. various covalent functionalization ... | 2016 | 27412259 |
| multiple adaptive routes of salmonella enterica typhimurium to biocide and antibiotic exposure. | biocides and antibiotics are used to eradicate or prevent the growth of microbial species on surfaces (occasionally on catheters), or infected sites, either in combination or sequentially, raising concerns about the development of co-resistance to both antimicrobial types. the effect of such compounds on salmonella enterica, a major food-borne and zoonotic pathogen, has been analysed in different studies, but only few works evaluated its biological cost, and the overall effects at the genomic an ... | 2016 | 27411385 |
| impact of pulsed light on cellular activity of salmonella enterica. | the objective of this study was a comprehensive characterization of physiological changes of salmonella enterica induced by intense broad spectrum pulsed light (pl). after exposing the bacteria to this nonthermal decontamination technology on a gel surface, multiple viability parameters beyond culturability were assessed. | 2016 | 27409040 |
| models of intestinal infection by salmonella enterica: introduction of a new neonate mouse model. | salmonella enterica serovar typhimurium is a foodborne pathogen causing inflammatory disease in the intestine following diarrhea and is responsible for thousands of deaths worldwide. many in vitro investigations using cell culture models are available, but these do not represent the real natural environment present in the intestine of infected hosts. several in vivo animal models have been used to study the host-pathogen interaction and to unravel the immune responses and cellular processes occu ... | 2016 | 27408697 |