Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antibacterial effect of calcium hydroxide on intraluminal and intratubular enterococcus faecalis. | root canal treatment involves the elimination of intraradicular microorganisms. calcium hydroxide [ca(oh)(2)] is the most widely used canal dressing material. enterococcus faecalis (e. faecalis) has been reported to be resistant to ca(oh)(2) in-vivo. the purpose of this study was to evaluate the efficacy of ca(oh)(2) on the elimination of intraluminal and intratubular e. faecalis. | 2011 | 23130062 |
| an in vitro comparative study on the antimicrobial effects of bioglass 45s5 vs. calcium hydroxide on enterococcus faecalis. | an ideal intracanal medicament should be able to eliminate any remaining intracanal microorganism. the aim of this study was to compare the antimicrobial effects of bioglass 45s5 with calcium hydroxide on enterococcus (e) faecalis in-vitro. | 2011 | 23130052 |
| in vitro scanning electron microscopic study on the effect of doxycycline and vancomycin on enterococcal induced biofilm. | enterococcus (e) faecalis bacteria adhere to dentine of teeth root canals to form the biofilm. e. faecalis has been shown to be resistant to antibiotics. this in vitro study aimed to investigate the efficacy of vancomycin and doxycycline in inhibiting e. faecalis biofilm formation. | 2010 | 23130027 |
| comparison of bacterial and dye microleakage of different root-end filling materials. | the provision of an effective apical seal is an essential factor when choosing an appropriate material for success of root-end surgeries. root-end resection, preparation and obturation should provide an adequate apical seal. the aim of this in vitro study was to investigate bacterial and dye microleakage of four different root-end filling materials and compare the efficacy of these two methods. | 2010 | 23130024 |
| comparative in vitro antibacterial activity of six root canal sealers against enterococcus faecalis and proteus vulgaris. | to evaluate the antimicrobial properties of six endodontic sealers in unset and set states against enterococcus faecalis and proteus vulgaris. | 2012 | 23129142 |
| antibacterial action of calcium hydroxide vehicles and calcium hydroxide pastes. | to evaluate the in vitro action of vehicles alone and with calcium hydroxide against different bacterial species. | 2012 | 23129141 |
| 3-phenyl substituted 6,7-dimethoxyisoquinoline derivatives as ftsz-targeting antibacterial agents. | the emergence of multidrug-resistant bacteria has created an urgent need for antibiotics with a novel mechanism of action. the bacterial cell division protein ftsz is an attractive target for the development of novel antibiotics. the benzo[c]phenanthridinium sanguinarine and the dibenzo[a,g]quinolizin-7-ium berberine are two structurally similar plant alkaloids that alter ftsz function. the presence of a hydrophobic functionality at either the 1-position of 5-methylbenzo[c]phenanthridinium deriv ... | 2012 | 23127490 |
| in vitro development and transfer of resistance to chlortetracycline in bacillus subtilis. | the present criteria and rules controlling the approval of the use of probiotics are limited to antibiotic resistance patterns and the presence of antibiotic resistance genes in bacteria. there is little information available in the literature regarding the risk of the usage of probiotics in the presence of antibiotic pressure. in this study we investigated the development and transfer of antibiotic resistance in bacillus subtilis selected in vitro by chlortetracycline in a stepwise manner. baci ... | 2012 | 23124749 |
| molecular structure, normal coordinate analysis, harmonic vibrational frequencies, natural bond orbital, td-dft calculations and biological activity analysis of antioxidant drug 7-hydroxycoumarin. | in this work, we report harmonic vibrational frequencies, molecular structure, nbo and homo, lumo analysis of umbelliferone also known as 7-hydroxycoumarin (7hc). the optimized geometric bond lengths and bond angles obtained by computation (monomer and dimmer) shows good agreement with experimental xrd data. harmonic frequencies of 7hc were determined and analyzed by dft utilizing 6-311+g(d,p) as basis set. the assignments of the vibrational spectra have been carried out with the help of normal ... | 2013 | 23123244 |
| ph and antimicrobial activity of portland cement associated with different radiopacifying agents. | objective. the aim of this study was to evaluate the antimicrobial activity and ph changes induced by portland cement (pc) alone and in association with radiopacifiers. methods. the materials tested were pure pc, pc + bismuth oxide, pc + zirconium oxide, pc + calcium tungstate, and zinc oxide and eugenol cement (zoe). antimicrobial activity was evaluated by agar diffusion test using the following strains: micrococcus luteus, streptococcus mutans, enterococcus faecalis, pseudomonas aeruginosa, an ... | 2012 | 23119173 |
| the effect of ascorbic acid on the substantivity of tetraclean in sodium hypochlorite-treated bovine dentin. | the purpose of this study was to assess the in vitro effect of ascorbic acid on the antibacterial substantivity of tetraclean in bovine root dentin pretreated with sodium hypochlorite (naocl). | 2012 | 23119132 |
| proteolytic activity by multiple bacterial species isolated from chronic venous leg ulcers degrades matrix substrates. | a major feature of chronic wounds is the loss of tissue, with the exposure of dermal components preventing primary closure and leading to bacterial colonization. bacterial colonization has been proposed as one of the common underlying pathologies present in chronic wounds. the objective of this exploratory study was to identify bacteria cultured from chronic venous leg ulcers and test for proteolytic activity that degrades matrix substrates. | 2013 | 23118301 |
| a surveillance of high-level gentamicin-resistant enterococcal bacteremia. | enterococci have recently been recognized as a causative organism of intractable infections, including severe sepsis and infective endocarditis, in immunocompromised patients. this study investigated the epidemiological, microbiological, and prognostic characteristics of high-level gentamicin-resistant (hlgr) enterococcal bacteremia, including severe cases of infective endocarditis, in japan. a total of 155 enterococcal bacteremia episodes were identified between july 2007 and december 2009. hlg ... | 2012 | 23115941 |
| the influence of chlorhexidine on the antibacterial effects of mta. | to investigate the influence of chlorhexidine in different concentrations when mixed with mineral trioxide aggregate gray (mta-g) or white (mta-w). the antibacterial effect on enterococcus faecalis was used as the primary parameter. | 2012 | 23115769 |
| evaluation of a pcr assay to detect enterococcusfaecalis in blood and determine glycopeptides resistance genes: van a and van b. | bacteremia due to enterococcus faecalis is usually caused by strains resistant to most antibiotics. effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. the aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococcal bacteremia and its sensitivity pattern to antimicrobial drugs. | 2012 | 23115452 |
| enterococcus faecalis inhibits hyphal morphogenesis and virulence of candida albicans. | the gram-positive bacterium enterococcus faecalis and the fungus candida albicans are both found as commensals in many of the same niches of the human body, such as the oral cavity and gastrointestinal (gi) tract. however, both are opportunistic pathogens and have frequently been found to be coconstituents of polymicrobial infections. despite these features in common, there has been little investigation into whether these microbes affect one another in a biologically significant manner. using a ... | 2013 | 23115035 |
| biochemical characterization of cardiolipin synthase mutations associated with daptomycin resistance in enterococci. | daptomycin (dap) resistance in enterococci has been linked to mutations in genes that alter the cell envelope stress response (cesr) (liafsr) and changes in enzymes that directly affect phospholipid homeostasis, and these changes may alter membrane composition, such as that of cardiolipin synthase (cls). while cls substitutions are observed in response to dap therapy, the effect of these mutations on cls activity remains obscure. we have expressed, purified, and characterized cls enzymes from bo ... | 2013 | 23114777 |
| whole-genome analysis of a daptomycin-susceptible enterococcus faecium strain and its daptomycin-resistant variant arising during therapy. | development of daptomycin (dap) resistance in enterococcus faecalis has recently been associated with mutations in genes encoding proteins with two main functions: (i) control of the cell envelope stress response to antibiotics and antimicrobial peptides (liafsr system) and (ii) cell membrane phospholipid metabolism (glycerophosphoryl diester phosphodiesterase and cardiolipin synthase [cls]). however, the genetic bases for dap resistance in enterococcus faecium are unclear. we performed whole-ge ... | 2012 | 23114757 |
| evaluation of bacterial microleakage of root canals irrigated with different irrigation solutions and ktp laser system. | the aim of this study was to evaluate bacterial microleakage of the root canals irrigated with different irrigation solutions and the potassium-titanyl-phosphate (ktp) laser system and filled with gutta-percha and ah26 root canal sealer. in addition, the effect of the irrigation solutions on dentin surface was evaluated with a scanning electron microscope (sem). | 2013 | 23113510 |
| counteraction of reactive oxygen species and determination of antibacterial efficacy of proanthocyanidin and lycopene when mixed with calcium hydroxide and chlorhexidine mixture: an in vitro comparative study. | the objective of the study was to determine the neutralizing effect of proanthocyanidin (grape seed extract) and lycopene (tomato extract) on reactive oxygen species (ros) generated by the mixture when used as an intracanal medicament. the study also evaluated the effect of proanthocyanidin and lycopene on the antibacterial efficacy of a mixture of chlorhexidine (chx) and calcium hydroxide [ca(oh)(2)] against enterococcus feacalis. | 2012 | 23112480 |
| molecular identification of an enterococcus faecalis endocarditis antigen efaa in root canals of therapy-resistant endodontic infections. | enterococcus faecalis has long been implicated in persistent root canal infections and therapy-resistant endodontic infections. it has also been associated with bacteremia, that is, infective endocarditis arising from certain invasive dental procedures. e. faecalis endocarditis antigen (efaa) has been identified as one of the principal virulence factors associated with infective endocarditis. | 2012 | 23112476 |
| comparative kinetic analysis of glycerol 3-phosphate cytidylyltransferase from enterococcus faecalis and listeria monocytogenes. | glycerol 3-phosphate cytidylyltransferase (gct) is an enzyme central to the synthesis of teichoic acids, components of the cell wall in gram positive bacteria. catalysis by gct from enterococcus faecalis and listeria monocytogenes has been investigated and catalytic properties compared. | 2012 | 23111733 |
| antimicrobial efficacy of cryotreatment against enterococcus faecalis in root canals. | the purpose of this investigation was to evaluate intracanal bacterial reduction by cryotreatment using a dental instrument equipped with a duct and connected to a cryogenic fluid source. a total of 86 roots were infected with enterococcus faecalis and incubated. after incubation, the contaminated roots were divided into three study groups: 35 roots irrigated with 2 ml of a 5% sodium hypochlorite (naocl) solution, 35 roots irrigated with 2 ml of a 5% naocl solution and further treated with cryo ... | 2013 | 23106670 |
| antibiotic susceptibility of periodontal enterococcus faecalis. | enterococcus faecalis may contribute to periodontal breakdown in heavily infected subgingival sites, particularly in patients responding poorly to mechanical forms of periodontal therapy. because only limited data are available on the antimicrobial sensitivity of enterococci of subgingival origin, this study evaluates the in vitro antibiotic susceptibility of e. faecalis isolated from periodontitis patients in the united states. | 2013 | 23106507 |
| characterization of the effects of an rpoc mutation that confers resistance to the fst peptide toxin-antitoxin system toxin. | overexpression of the fst toxin in enterococcus faecalis strain og1x leads to defects in chromosome segregation, cell division and, eventually, membrane integrity. the m7 mutant derivative of og1x is resistant to most of these effects but shows a slight growth defect in the absence of fst. full-genome sequencing revealed two differences between m7 and its og1x parent. first, og1x contains a frameshift mutation that inactivates the etar response regulator gene, while m7 is a wild-type revertant f ... | 2013 | 23104812 |
| synthesis and characterization of a series of transition metal complexes with a new symmetrical polyoxaaza macroacyclic schiff base ligand: x-ray crystal structure of cobalt(ii) and nickel(ii) complexes and their antibacterial properties. | a new symmetrical [n4o2] hexadentate schiff base ligand, (e)-n-(pyridin-2-ylmethylene)-2-(3-(2-((e)-pyridin-2-lmethyleneamino)phenoxy)naphthalen-2-yloxy)benzenamine, abbreviated to l, and its complexes of ni(ii), cu(ii), zn(ii), co(ii), cd(ii) and mn(ii) have been synthesized in the presence of metal ions. the complexes were structurally characterized by elemental analyses, ir, uv-vis, nmr and molar conductivity. the crystal structures of two complexes, [nil(ono2)2]·2h2o and [colcl2]ch3oh·0.5h2o ... | 2013 | 23099161 |
| bactericidal strontium-releasing injectable bone cements based on bioactive glasses. | strontium-releasing injectable bone cements may have the potential to prevent implant-related infections through the bactericidal action of strontium, while enhancing bone formation in patients suffering from osteoporosis. a melt-derived bioactive glass (bg) series (sio2–cao–caf2–mgo) with 0–50% of calcium substituted with strontium on a molar base were produced. by mixing glass powder, poly(acrylic acid) and water, cements were obtained which can be delivered by injection and set in situ, givin ... | 2013 | 23097502 |
| effectiveness of photoactivated disinfection (pad) to kill enterococcus faecalis in planktonic solution and in an infected tooth model. | the aim of this study was to investigate the effectiveness of photoactivated disinfection (pad) in killing enterococcus faecalis (ef) in planktonic solution and in an infected tooth model. | 2012 | 23095248 |
| antimicrobial effects of root canal medicaments against enterococcus faecalis and streptococcus mutans. | to compare the antimicrobial activities of activ point (roeko, langenau, germany), calcium hydroxide plus point (roeko, langenau, germany), calcium hydroxide, 1% chlorhexidine gel and bioactive glass (s53p4) against enterococcus faecalis and streptococcus mutans. | 2013 | 23095092 |
| acute gastrointestinal dilation in laboratory rhesus monkeys in the korea national primate research center. | acute gastrointestinal dilation is a medical condition in which the stomach and intestine become overstretched by excessive gas content. in laboratory monkeys, cases of bloating involving gastrointestinal dilation are rarely seen, and the cause thereof is not clearly defined. two rhesus monkeys in the korea national primate research center were found to suffer from acute gastrointestinal dilation. one of the monkeys showed severe gastric bloating after recovering from general anesthesia with iso ... | 2012 | 23091523 |
| smad4 haploinsufficiency associates with augmented colonic inflammation in select humans and mice. | smad4 is a common mediator of the tgf-beta signaling pathway. one of the members of this pathway, tgf-beta 1, has an important role in controlling gut inflammation in relation to the continuous stimulation of the intestinal microbiota. smad4 haploinsufficiency in humans has been linked to juvenile polyposis hereditary hemorrhagic telangiectasia syndrome (jp/hht; omim#17505). hematochezia and colonic mucosal inflammation suggestive of inflammatory bowel diseases (ibd) have been reported in jp/hht ... | 2012 | 23090737 |
| characterization of plasmid pml21 of enterococcus faecalis ml21 from koumiss. | 2013 | 23090644 | |
| psk41-like plasmid is necessary for inc18-like vana plasmid transfer from enterococcus faecalis to staphylococcus aureus in vitro. | vancomycin-resistant staphylococcus aureus (vrsa) is thought to result from the in vivo conjugative transfer of a vana plasmid from an enterococcus sp. to s. aureus. we studied bacterial isolates from vrsa cases that occurred in the united states to identify microbiological factors which may contribute to this plasmid transfer. first, vancomycin-susceptible, methicillin-resistant s. aureus (mrsa) isolates from five vrsa cases were tested for their ability to accept foreign dna by conjugation in ... | 2013 | 23089754 |
| synthesis and characterization of polysaccharide-maghemite composite nanoparticles and their antibacterial properties. | the aim of this study was to obtain saccharide (dextran and sucrose)-coated maghemite nanoparticles with antibacterial activity. the polysaccharide-coated maghemite nanoparticles were synthesized by an adapted coprecipitation method. x-ray diffraction (xrd) studies demonstrate that the obtained polysaccharide-coated maghemite nanoparticles can be indexed into the spinel cubic lattice with a lattice parameter of 8.35 å. the refinement of xrd spectra indicated that no other phases except the maghe ... | 2012 | 23088756 |
| human serum albumin stabilized gold nanoclusters as selective luminescent probes for staphylococcus aureus and methicillin-resistant staphylococcus aureus. | in this work, human serum albumin (hsa) stabilized gold nanoclusters (hsa-auncs) with reddish photoluminescence were used as sensing probes for pathogenic bacteria including enterobacter cloacae, escherichia coli j96, pseudomonas aeruginosa, pandrug-resistant acinetobacter baumannii (pdrab), staphylococcus aureus, methicillin-resistant s. aureus (mrsa), streptococcus pyogenes, and vancomycin-resistant enterococcus faecalis (vre). we discovered that hsa-auncs have unique affinity with s. aureus a ... | 2012 | 23088348 |
| antibacterial activity of the venom of heterometrus xanthopus. | heterometrus xanthopus (scorpion) is one of the most venomous and ancient arthropods. its venom contains anti-microbial peptides like hadrurin, scorpine, pandinin 1, and pandinin 2 that are able to effectively kill multidrug-resistant pathogens. the present study was conducted to evaluate the anti-bacterial activity of h. xanthopus venom. six gram-positive and gram-negative bacterial strains were tested against 1/100, 1/10, and 1/1 fractions of distilled water diluted and crude venom. 1/100 and ... | 2015 | 23087515 |
| development of quantitative pcr assays targeting the 16s rrna genes of enterococcus spp. and their application to the identification of enterococcus species in environmental samples. | the detection of environmental enterococci has been determined primarily by using culture-based techniques that might exclude some enterococcal species as well as those that are nonculturable. to address this, the relative abundances of enterococci were examined by challenging fecal and water samples against a currently available genus-specific assay (entero1). to determine the diversity of enterococcal species, 16s rrna gene-based group-specific quantitative pcr (qpcr) assays were developed and ... | 2013 | 23087032 |
| cspr, a cold shock rna-binding protein involved in the long-term survival and the virulence of enterococcus faecalis. | by coprecipitation, we identified rna-binding proteins in the gram-positive opportunistic pathogen enterococcus faecalis known to be deficient of the rna chaperone hfq. in particular, we characterized one belonging to the cold shock protein (csp) family (ef2925) renamed cspr for cold shock protein rna binding. compared to the wild-type strain, the δcspr mutant was less virulent in an insect infection model (galleria mellonella) and exhibited a decreased persistence in mouse kidneys and a low sur ... | 2012 | 23086208 |
| antibacterial activity of substituted 5-methylbenzo[c]phenanthridinium derivatives. | antibiotic resistance has prompted efforts to discover antibiotics with novel mechanisms of action. ftsz is an essential protein for bacterial cell division, and has been viewed as an attractive target for the development of new antibiotics. sanguinarine is a benzophenanthridine alkaloid that prevents cytokinesis in bacteria by inhibiting ftsz self-assembly. in this study, a series of 5-methylbenzo[c]phenanthridinium derivatives were synthesized and evaluated for antibacterial activity against s ... | 2012 | 23084900 |
| characterization of lead-resistant river isolate enterococcus faecalis and assessment of its multiple metal and antibiotic resistance. | contamination of surface waters has a direct impact on the public health of entire communities. microorganisms inhabiting contaminated surface waters have developed mechanisms of coping with a variety of toxic metals and drugs. investigations were carried out to isolate and identify lead-resistant bacteria from the river kızılırmak along the city of kırıkkale, turkey. of the 33 lead-resistant isolates, one isolate with a minimal inhibitory concentration of 1,200 mg l(-1) was isolated and identif ... | 2013 | 23079796 |
| antimicrobial efficacy of a human β-defensin-3 peptide using an enterococcus faecalis dentine infection model. | to assess the antibacterial efficacy of a human β-defensin-3 (hbd3) peptide against enterococcus faecalis biofilms. | 2013 | 23078156 |
| [multivalvular infective endocarditis with enterococcus faecalis]. | infective endocarditis (ie) is a relatively rare disease that can take part of sepsis and may be a diagnostic and therapeutic challenge. polyvalvular location of el is rare. we present a patient of 60 years diagnosed with plurivalvular infective endocarditis by enterococcus faecalis with septic complications, visceral, dragged, hospitalized for a psychiatric pathology, who raised the problems of diagnosis and treatment, without being able to make assessments on the entrance gate. for help in dia ... | 2016 | 23077930 |
| the evaluation of antimicrobial susceptibility of urine enterococci with the vitek 2 automated system in eastern turkey. | antibiotic resistant enterococci are an emerging problem, especially in urinary tract infections. the aim of the present study was to evaluate the antimicrobial susceptibility of 118 enterococci isolates from urine samples of patients admitted to malatya state hospital, a secondary care hospital in eastern turkey. the vitek 2 automated sytem was used to identify the bacteria and detect antimicrobial susceptibility to ten antibiotics: ampicillin, imipenem, ciprofloxacin, moxifloxacin, quinupristi ... | 2012 | 23077822 |
| rapid discrimination of gram-positive and gram-negative bacteria in liquid samples by using naoh-sodium dodecyl sulfate solution and flow cytometry. | for precise diagnosis of urinary tract infections (uti), and selection of the appropriate prescriptions for their treatment, we explored a simple and rapid method of discriminating gram-positive and gram-negative bacteria in liquid samples. | 2012 | 23077549 |
| false daptomycin-nonsusceptible mic results by microscan panel pc 29 relative to etest results for staphylococcus aureus and enterococci. | this study correlated the daptomycin mic results obtained by microscan and by etest in staphylococcus aureus and enterococci and found that the microscan panel gp 29 had a high rate of false nonsusceptible results. of the isolates interpreted as nonsusceptible by microscan, 87% of staphylococcus aureus, 90% of enterococcus faecalis, and 88% of enterococcus faecium isolates were interpreted as susceptible by etest. in turn, etest also has false nonsusceptible results compared to reference methods ... | 2013 | 23077121 |
| intrinsic and acquired resistance mechanisms in enterococcus. | enterococci have the potential for resistance to virtually all clinically useful antibiotics. their emergence as important nosocomial pathogens has coincided with increased expression of antimicrobial resistance by members of the genus. the mechanisms underlying antibiotic resistance in enterococci may be intrinsic to the species or acquired through mutation of intrinsic genes or horizontal exchange of genetic material encoding resistance determinants. this paper reviews the antibiotic resistanc ... | 2012 | 23076243 |
| study of photodynamic effects on nih 3t3 cell line and bacteria. | bacterial resistance to antibiotics is a constantly growing challenge. photodynamic therapy (pdt) offers a new approach to the treatment of bacterial and viral diseases. the aim of this study was to compare the efficacy of photosensitizers used in pdt applied to cell lines and bacterial strains. | 2014 | 23073531 |
| antibiotic producing microorganisms from river wiwi, lake bosomtwe and the gulf of guinea at doakor sea beach, ghana. | microorganisms have provided a wealth of metabolites with interesting activities such as antimicrobial, antiviral and anticancer. in this study, a total of 119 aquatic microbial isolates from 30 samples (taken from water bodies in ghana) were screened by the agar-well diffusion method for ability to produce antibacterial-metabolites. | 2012 | 23072432 |
| species-level analysis of dna sequence data from the nih human microbiome project. | outbreaks of antibiotic-resistant bacterial infections emphasize the importance of surveillance of potentially pathogenic bacteria. genomic sequencing of clinical microbiological specimens expands our capacity to study cultivable, fastidious and uncultivable members of the bacterial community. herein, we compared the primary data collected by the nih's human microbiome project (hmp) with published epidemiological surveillance data of staphylococcus aureus. | 2012 | 23071716 |
| comparative genomics of community-acquired st59 methicillin-resistant staphylococcus aureus in taiwan: novel mobile resistance structures with is1216v. | methicillin-resistant staphylococcus aureus (mrsa) with st59/sccmecv and panton-valentine leukocidin gene is a major community-acquired mrsa (ca-mrsa) lineage in taiwan and has been multidrug-resistant since its initial isolation. in this study, we studied the acquisition mechanism of multidrug resistance in an st59 ca-mrsa strain (pm1) by comparative genomics. pm1's non-β-lactam resistance was encoded by two unique genetic traits. one was a 21,832-bp composite mobile element structure (mes(pm1) ... | 2012 | 23071689 |
| epidemiology of vancomycin-resistant enterococcus faecalis: a case-case-control study. | although much is known about vancomycin-resistant (vr) enterococcus faecium, little is known about the epidemiology of vr enterococcus faecalis. the predilection of vr e. faecalis to transfer the vancomycin resistance determinant to staphylococcus aureus is much greater than that of vr e. faecium. the epidemiology of vr e. faecalis has important implications regarding the emergence of vancomycin-resistant s. aureus (vrsa); 8 of 13 reported vrsa cases have been from michigan. a retrospective case ... | 2013 | 23070173 |
| transferable multiresistance plasmids carrying cfr in enterococcus spp. from swine and farm environment. | seventy-seven porcine enterococcus isolates with florfenicol mics of ≥16 μg of were/ml screened for the presence of the multiresistance gene cfr, its location on plasmids, and its genetic environment. three isolates-enterococcus thailandicus 3-38 (from a porcine rectal swab collected at a pig farm), enterococcus thailandicus w3, and enterococcus faecalis w9-2 (the latter two from sewage at a different farm), carried the cfr gene. the smai pulsed-field gel electrophoresis patterns of the three is ... | 2013 | 23070165 |
| activity of moxifloxacin, imipenem, and ertapenem against escherichia coli, enterobacter cloacae, enterococcus faecalis, and bacteroides fragilis in monocultures and mixed cultures in an in vitro pharmacokinetic/pharmacodynamic model simulating concentrations in the human pancreas. | the activities of moxifloxacin, imipenem, and ertapenem against pathogens causing severe necrotizing pancreatitis were studied in an in vitro pharmacokinetics/pharmacodynamics (pk/pd) model. escherichia coli, enterobacter cloacae, enterococcus faecalis, and bacteroides fragilis were exposed in monocultures and mixed cultures to concentrations of the three agents comparable to those in the human pancreas. moxifloxacin was more active than the two carbapenems in monocultures and mixed cultures, re ... | 2012 | 23070164 |
| synergistic effect of microbubble emulsion and sonic or ultrasonic agitation on endodontic biofilm in vitro. | irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. this study examined the effects of microbubble emulsion (me) combined with sonic or ultrasonic agitation on irrigation dynamics and reduction of biofilm bacteria within root canal models. | 2012 | 23063230 |
| wetting properties and critical micellar concentration of benzalkonium chloride mixed in sodium hypochlorite. | the purposes of the present study were to (1) assess the effect of the addition of benzalkonium chloride to sodium hypochlorite on its wetting properties, contact angle, and surface energy; (2) determine the critical micellar concentration of benzalkonium chloride in sodium hypochlorite; and (3) investigate the influence of addition of benzalkonium chloride on the free chlorine level, cytotoxicity, and antiseptic properties of the mixture. | 2012 | 23063229 |
| crispr-cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired enterococcus faecalis. | microorganisms are vulnerable to invasion by mobile genetic elements such as viruses, plasmids, and transposons. the recently discovered clustered regularly interspaced short palindromic repeats (crispr)-associated system, or crispr-cas, is an adaptive immunity system found in most archaea and many bacteria that targets and inactivates invading foreign genetic elements. cells with crispr-cas are more likely to resist the invasion and uptake of foreign dna such as viruses, plasmids, and transposo ... | 2012 | 23063226 |
| evaluation of microbial survival post-incidence on fresh mozzarella cheese. | commercial fresh mozzarella cheese is made by direct acidification and is stored dry or in water without salt addition. the cheese has a shelf life of 6 wk, but usually develops an off-flavor and loses textural integrity by 4 wk, potentially due to the lack of salt and high moisture that allow the outgrowth of undesirable bacteria. to understand how microbial incidence affects cheese quality and how incident pathogen-related bacteria are limited by salt level during refrigerated storage, we made ... | 2012 | 23063153 |
| a comparative study to determine the recovery rate of microorganisms of bloodstream infections: two versus three blood culture specimens. | there has been a development of automated and continuous-monitoring blood culture systems that are more sensitive than conventional systems for the detection of microorganisms. whether two or three blood culture specimens obtained during a 24-hour period using these automated systems achieving a higher recovery rate of microorganism remains to be determined. the present study was aimed to compare the recovery rates of microorganism of blood-stream infections (bsis) using two and three blood cult ... | 2012 | 23061310 |
| the par toxin-antitoxin system from enterococcus faecalis plasmid pad1 and its chromosomal homologs. | the par post-segregational killing locus present on enterococcus faecalis plasmid pad1 was the first type i toxin-antitoxin system described in gram-positive bacteria. translation of the 33 amino acid fst toxin, encoded on rna i, is suppressed by a 66 nucleotide regulatory rna, rna ii. rna i and rna ii are transcribed convergently and interact at dispersed regions of complementarity, establishing a stable complex that accumulates in plasmid-containing cells. rna ii is slowly removed from the com ... | 2012 | 23059908 |
| antibacterial activity of substituted dibenzo[a,g]quinolizin-7-ium derivatives. | berberine is a substituted dibenzo[a,g]quinolizin-7-ium derivative whose modest antibiotic activity is derived from its disruptive impact on the function of the essential bacterial cell division protein ftsz. the present study reveals that the presence of a biphenyl substituent at either the 2- or 12-position of structurally-related dibenzo[a,g]quinolizin-7-ium derivatives significantly enhances antibacterial potency versus staphylococcus aureus and enterococcus faecalis. studies with purified s ... | 2012 | 23058886 |
| propolis: a new alternative for root canal disinfection. | this study evaluated and compared colony forming units (cfus) and minimum inhibitory concentrations (mics) of calcium hydroxide and propolis as intracanal medicaments. | 2012 | 23056131 |
| is chlorhexidine an ideal vehicle for calcium hydroxide? a microbiologic review. | microorganisms play a major role in the initiation and perpetuation of pulpal and periapical disease. in order to predictably achieve a bacteria-free root canal system, it is necessary to use intracanal medicaments. calcium hydroxide [ca(oh)(2)] is the most common intracanal medicaments. it is effective against primary infections. however, its effectiveness against enterococcus (e.) faecalis and candida (c.) albicans is controversial. on the other hand, chlorhexidine (chx) is a potent agent agai ... | 2012 | 23056129 |
| the effect of heat-killed candida albicans and dentin powder on the antibacterial activity of chlorhexidine solution. | the purpose of this study was to compare the inhibitory effect of heat-killed candida albicans and dentin powder on the antibacterial activity of chlorhexidine (chx) against enterococcus faecalis and streptococcus sanguis. | 2012 | 23056121 |
| removal of faecal indicator pathogens from waters and wastewaters by photoelectrocatalytic oxidation on tio(2)/ti films under simulated solar radiation. | the disinfection efficiency of water and secondary treated wastewater by means of photoelectrocatalytic oxidation (pec) using reference strains of enterococcus faecalis and escherichia coli as faecal indicators was evaluated. operating parameters such as applied potential (2-10 v), initial bacterial concentration (10(3)-10(7) cfu/ml), treatment time (up to 90 min) and aqueous matrix (pure water and treated effluent) were assessed concerning their impact on disinfection. | 2012 | 23054740 |
| antifungal activity and composition of essential oils of conyza canadensis herbs and roots. | essential oils from herbs and roots of conyza canadensis (horseweed), collected in hungary, were obtained by hydrodistillation. the chemical compositions of the oils were analysed by combination of gc and gc/ms. the major constituent of the oil obtained from the aerial parts of horseweed was limonene (78%), while the main component of root oil was 2z,8z-matricaria ester. the antimicrobial activities of the oils were tested on gram-positive (enterococcus faecalis, staphylococcus aureus, and strep ... | 2012 | 23049473 |
| a composite bacteriophage alters colonization by an intestinal commensal bacterium. | the mammalian intestine is home to a dense community of bacteria and its associated bacteriophage (phage). virtually nothing is known about how phages impact the establishment and maintenance of resident bacterial communities in the intestine. here, we examine the phages harbored by enterococcus faecalis, a commensal of the human intestine. we show that e. faecalis strain v583 produces a composite phage (φv1/7) derived from two distinct chromosomally encoded prophage elements. one prophage, prop ... | 2012 | 23045666 |
| draft genome sequence of the quality control strain enterococcus faecalis atcc 29212. | enterococcus faecalis atcc 29212, a vancomycin-sensitive strain, has been extensively used as a representative control strain for clinical and laboratory experiments. here we report the draft genome and annotation of this strain, containing 3,027,060 bp, with a g+c content of 37.2% in 126 contigs (≥ 500 bp). | 2012 | 23045510 |
| virulence factors in isolates of enterococcus faecalis from infective endocarditis and from the normal flora. | infective endocarditis (ie) is a severe infection associated with significant morbidity and mortality. enterococcus faecalis is among the most common causes of this disease, and yet little is known about the pathogenesis of e. faecalis ie. we screened 21 e. faecalis isolates from ie and 21 isolates from normal flora for the putative virulence factors ace, asa1, gele, and esp with pcr. in addition, we determined the ability of the isolates to form biofilm and to aggregate platelets. with the exce ... | 2013 | 23044056 |
| the changing epidemiology of bacteraemias in europe: trends from the european antimicrobial resistance surveillance system. | we investigated bacteraemia trends for five major bacterial pathogens, staphylococcus aureus, escherichia coli, streptococcus pneumoniae, enterococcus faecalis and enterococcus faecium, and determined how expanding antimicrobial resistance influenced the total burden of bacteraemias in europe. aetiological fractions of species and antibiotic phenotypes were extracted from the european antimicrobial resistance surveillance system (earss) database for laboratories, which consistently reported betw ... | 2013 | 23039210 |
| the effect of a wound care solution containing polyhexanide and betaine on bacterial counts: results of an in vitro study . | polyhexanide and betaine topical solution is used in the management of infected wounds as a cleaning agent. an in vitro study was conducted to examine the antimicrobial effects of a solution containing 0.1% of the antimicrobial agent polyhexanide and 0.1% of the surfactant betaine. three batches of each product were tested, and culture results of 13 microorganisms were evaluated after 7, 14, and 28 days using usp <51> methodology. growth reduction was identical at each day following exposure to ... | 2012 | 23037330 |
| impact of ph on activity of trimethoprim, fosfomycin, amikacin, colistin and ertapenem in human urine. | although major impairment of activity at lower ph values has been reported for fluoroquinolones, acidification is a widely recommended practice for the prophylaxis and treatment of uncomplicated urinary tract infections (utis). until now, there is little evidence for the influence of ph on the activity on other antimicrobial classes in urine. | 2012 | 23037005 |
| computer keyboard covers impregnated with a novel antimicrobial polymer significantly reduce microbial contamination. | contaminated computer keyboards have been acknowledged as a potential source for bacterial transmission between health care providers and patients. biosafe hm 4100 is an antimicrobial polymer that can be incorporated into the polyurethane material used to make keyboard covers. this study aimed to determine whether plastic keyboard covers containing hm 4100 effectively minimize the survival of bacterial species commonly present on health care environmental surfaces. | 2013 | 23036480 |
| effects of a pulsed light-induced stress on enterococcus faecalis. | pulsed light (pl) technology is a surface decontamination process that can be used on food, packaging or water. pl efficiency may be limited by its low degree of penetration or because of a shadow effect. in these cases, surviving bacteria will be able to perceive pl as a stress. such a stress was mimicked using low transmitted energy conditions, and its effects were investigated on the highly environmental adaptable bacterium enterococcus faecalis v583. | 2013 | 23035907 |
| the vane operon in enterococcus faecalis n00-410 is found on a putative integrative and conjugative element, tn6202. | to date no complete genetic structure of acquired dna harbouring a d-ala-d-ser operon in an enterococcus is known. we wished to characterize the acquired dna harbouring the vane operon located in the enterococcus faecalis n00-410 chromosome. | 2013 | 23034711 |
| enterococcus faecalis leakage of root canal sealers: an ex vivo study. | the aim of this ex vivo study was to evaluate bacterial penetration after filling root canals using 3 different techniques. three experimental groups of 25 teeth each, obturated with lateral-warm-vertical condensation of gutta-percha, microseal technique and endorez system, respectively, were tested in a split chamber model system using enterococcus faecalis and monitored for 180 days to determine bacterial penetration. a statistical analysis was performed using the kaplan-meier method. median s ... | 2012 | 23034274 |
| the relative importance of staphylococcus saprophyticus as a urinary tract pathogen: distribution of bacteria among urinary samples analysed during 1 year at a major swedish laboratory. | to determine the distribution of urinary tract pathogens with focus on staphylococcus saprophyticus and analyse the seasonality, antibiotic susceptibility, and gender and age distributions in a large swedish cohort. s. saprophyticus is considered an important causative agent of urinary tract infection (uti) in young women, and some earlier studies have reported up to approximately 40% of utis in this patient group being caused by s. saprophyticus. we hypothesized that this may be true only in ve ... | 2013 | 23030816 |
| survey on hospital-acquired urinary tract infection in neurological intensive care unit. | this study aimed to explore the causes, incidence, and risk factors of urinary tract infection patients in neurological intensive care unit (icu). patients (n = 916) admitted to the neurological icu from january 2005 to december 2010 were retrospectively surveyed for urinary tract infections. there were 246 patients in neurological icu who were diagnosed with hospital-acquired urinary tract infection during that period of time (26.9%). forty-three cases were upper urinary tract infection, and 20 ... | 2013 | 23030808 |
| a case of daptomycin-induced immune thrombocytopenia. | we report a case of severe daptomycin-induced immune thrombocytopenia in a patient treated for methicillin-resistant staphylococcus epidermidis and ampicillin-resistant enterococcus faecalis bacteremia acquired in an intensive care unit. serum antibodies bound to platelets in the presence of daptomycin on flow cytometry. there was no evidence of other causes of thrombocytopenia. the patient died of brain herniation complicating extensive cerebral hemorrhage. to our knowledge, this is the first d ... | 2012 | 23027187 |
| water disinfection using photo-fenton: effect of temperature on enterococcus faecalis survival. | the photo-fenton process is a promising alternative to classical water disinfection treatments, although information in this regard is scarce due to its operational limitations. the effect of temperature (10, 20, 30 and 40 °c) was studied on water disinfection using the photo-fenton reaction at initial near neutral ph with resorcinol as a model of natural organic matter (nom). enterococcus faecalis, a gram-positive microorganism, was selected as an indicator of wastewater faecal contamination. t ... | 2012 | 23021523 |
| evaluation of genetic pattern of gentamicin-resistant enterococci isolated from clinical samples. | enterococci are among resistant microorganisms to antibiotics and are responsible for some of acute gingival infections. this study aimed at molecular evaluation of gentamicin-resistant enterococcus species and determining the resistance genes. | 2011 | 23019503 |
| antibacterial mechanisms of rhodomyrtone against important hospital-acquired antibiotic-resistant pathogenic bacteria. | the antibacterial mechanisms of rhodomyrtone, a member of the acylphloroglucinols isolated from rhodomyrtus tomentosa leaves, against important hospital-acquired antibiotic-resistant pathogenic bacteria were assessed. the results indicated that rhodomyrtone exhibited pronounced antibacterial activity against key antibiotic-resistant pathogens including epidemic meticillin-resistant staphylococcus aureus (emrsa), vancomycin-intermediate s. aureus and vancomycin-resistant enterococcal strains. the ... | 2013 | 23019189 |
| synergistic bactericidal activity between hyperosmotic stress and membrane-disrupting nanoemulsions. | there is a clear clinical need for alternative types of non-antibiotic biocides due to the rising global health concern of microbial drug resistance. in this work, a novel antibacterial concept was delineated that utilized hyperosmotic stress (h) in concert with membrane-disrupting nanoemulsions (nes). the antibacterial effects of either h or a ne, as well as in combination (h+ne), were assessed in vitro using an escherichia coli model. it was found that exposure to h or ne alone produced dose-d ... | 2013 | 23019188 |
| quantification of antibiotic in biofilm-inhibiting multilayers by 7.87 ev laser desorption postionization ms imaging. | the potential of laser desorption postionization mass spectrometry (ldpi-ms) imaging for small molecule quantification is demonstrated here. the n-methylpiperazine acetamide (mpa) of ampicillin was adsorbed into polyelectrolyte multilayer surface coatings composed of chitosan and alginate, both high molecular weight biopolymers. these mpa-ampicillin spiked multilayers were then shown to inhibit the growth of enterococcus faecalis biofilms that play a role in early stage infection of implanted me ... | 2012 | 23017064 |
| antimicrobial efficacy of metapex (calcium hydroxide with iodoform formulation) at different concentrations against selected microorganisms--an in vitro study. | the objective of this study was to assess, in vitro, the effectiveness of several concentrations of metapex (0.22 gm/ml, 0.022 gm/ml, 0.0022 gm/ml) in the elimination of selected microorganisms. different concentrations of metapex were prepared by dissolving it in ethanol (99.9%) pre-sterilized whatman paper discs, 6 mm in diameter and soaked with the test solution, were prepared and placed onto the previously seeded agar petri plates. plates were incubated aerobically for enterococcus faecalis ... | 2011 | 23016485 |
| aerobic vaginitis and mixed infections: comparison of clinical and laboratory findings. | to investigate the clinical features of aerobic vaginitis (av) and mixed infections with av to achieve efficient diagnosis. | 2013 | 23015152 |
| in vivo exposure of mytilus edulis to living enteric bacteria: a threat for immune competency? | mussels are widespread in coastal environments and experience various physical, chemical, and bacteriological conditions. owing to the increase of coastal urbanization, mussels are now commonly exposed not only to indigenous bacteria, but also to enteric bacteria originating from pulsed and chronic sewage discharges into coastal environments. due to its broad resilience to environmental variations, the blue mussel mytilus edulis is commonly used as an indicator of environmental quality in bio-mo ... | 2013 | 23014953 |
| comparative analysis of classical and molecular microbiology methods for the detection of escherichia coli and enterococcus spp. in well water. | the microbiological quality of 165 1 litre well water samples collected in the québec city region was assessed by culture-based methods (mfc agar, chromocult coliform agar, colilert(®), mi agar, chromocult enterococci, enterolert™, and mei agar) and by a molecular microbiology strategy, dubbed crename-rtpcr, developed for the detection of escherichia coli, enterococcus spp., enterococcus faecalis/faecium, and bacillus atrophaeus subsp. globigii. in these drinking water samples, approved culture- ... | 2012 | 23014932 |
| the influence of predation and competition on the survival of commensal and pathogenic fecal bacteria in aquatic habitats. | the role of fecal indicator bacteria (fib) in water quality assessment is to provide a warning of the increased risk of pathogen presence. an effective surrogate for waterborne pathogens would have similar survival characteristics in aquatic environments. although the effect of abiotic factors such as sunlight and salinity on the survival of fib and pathogens are becoming better understood, the effect of the indigenous microbiota is not well characterized. the influence of biotic factors on the ... | 2013 | 23013262 |
| the effect of temperature and contact time of sodium hypochlorite on human roots infected with enterococcus faecalis and candida albicans. | the aim of this study was to evaluate the effectiveness of 2.5 % naocl at different temperature and time intervals on enterococcus faecalis and candida albicans-infected human roots. a total of 112 root cylinders prepared from extracted single-rooted humans were infected by e. faecalis (group a, n = 56) or c. albicans (group b, n = 56); 3 root cylinders served as negative controls. both groups were further divided into 6 subgroups according to three contact times (30 s, 1 min, 5 min) with naocl ... | 2014 | 23011474 |
| bactericidal effects of nd:yag laser irradiation and sodium hypochlorite solution on enterococcus faecalis biofilm. | the aim of this study was to evaluate the bactericidal effects of nd:yag laser on biofilm of enterococcus faecalis. | 2012 | 23003122 |
| ozone in dentistry: microbiological effects of gas action depending on the method and the time of application using the ozonytron device. experimental study. | bactericidal, virucidal, and fungicidal activity of ozone is generally known and has been exploited for years in industry and medicine. ozone therapy became an inherent element of the treatment of infection in such fields as surgery, dermatology, cosmetics, and dentistry. ozone is a gas, so it penetrates very well even into such tissues and spaces that are not easily accessible. thanks to the property of deep penetration and long ozone presence in tissues after treatment, a long-lasting bacteric ... | 2011 | 23002675 |
| clinical analysis of cataract surgery complicated by endophthalmitis. | to assess the efficacy of vitrectomy combined with intravitreal injection in the treatment of endophthalmitis after phacoemulsification and iol implantation. | 2012 | 22993061 |
| the antibacterial efficacy of biopure mtad in root canal contaminated with enterococcus faecalis. | aim. the purpose of this in vitro study was to assess the antimicrobial efficacy of biopure mtad against e. faecalis in contaminated root canals. materials and methods. forty-two single rooted extracted human teeth were inoculated with e. faecalis and incubated for four weeks. the samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (naocl); 3% naocl; biopure mtad; 1.5% naocl/17% edta; or 3% naocl/17% edta. after a one-week incubation, ... | 2012 | 22991671 |
| rapidly progressive glomerulonephritis associated with pr3-anca positive subacute bacterial endocarditis. | patients with bacterial endocarditis often have renal complications. this report presents the case of an elderly man with rapidly progressive glomerulonephritis (rpgn) associated with subacute bacterial endocarditis (sbe) due to enterococcus faecalis infection. the patient was positive for anti-proteinase 3-antineutrophil cytoplasmic antibody (pr3-anca) and rheumatoid factor (rf) with hypocomplementemia. treatment for sbe with antibiotics and the surgical replacement of the affected valves resul ... | 2012 | 22989831 |
| [spectrum and drug sensitivity of pathogenic bacteria in children with nephrotic syndrome complicated by urinary tract infection: an analysis of 97 cases]. | to investigate the spectrum and drug sensitivity of pathogenic bacteria in children with nephrotic syndrome (ns) complicated by urinary tract infection (uti). | 2012 | 22989432 |
| inhibition of listeria monocytogenes by enterococcus mundtii isolated from soil. | two bacterial isolates with inhibitory activity against listeria monocytogenes and enterococcus faecalis were obtained from soil. genotypic and phenotypic characterization identified them as enterococcus mundtii, a species whose ability to compete with l. monocytogenes is relatively unexplored compared to other members of the genus. the thermal stability of the inhibitory factor and its sensitivity to proteolytic enzymes indicate that it is most likely a bacteriocin. both isolates grew at compar ... | 2012 | 22986201 |
| sodium chloride and potassium sorbate: a synergistic combination against enterococcus faecalis biofilms: an in vitro study. | incomplete disinfection of the root canal system is a major cause of post-treatment disease. this study aimed to investigate the disinfecting property of organic acid salts and sodium chloride (nacl), in a double-hurdle strategy, on enterococcus faecalis biofilms. first of all, the high-throughput resazurin metabolism assay (rma) was used to test a range of organic acid salts. then, to gain more insight into the efficacy of sorbate salt solutions, 48-h e. faecalis biofilms were evaluated in colo ... | 2012 | 22985004 |
| validation of adenosine triphosphate to audit manual cleaning of flexible endoscope channels. | compliance with cleaning of flexible endoscope channels cannot be verified using visual inspection. adenosine triphosphate (atp) has been suggested as a possible rapid cleaning monitor for flexible endoscope channels. there have not been published validation studies to specify the level of atp that indicates inadequate cleaning has been achieved. | 2013 | 22980510 |
| infection of apical dentin and root-end cavity disinfection. | the purpose of this study was to assess enterococcus faecalis penetration into the dentin of the apical 3 mm and bacterial death after the application of either chlorhexidine or laser to root-end cavities. | 2012 | 22980184 |
| effectiveness of endodontic disinfecting solutions against young and old enterococcus faecalis biofilms in dentin canals. | enterococcus faecalis is a species commonly isolated from persistent root canal infections. the purpose of this study was to compare the antibacterial effects of different disinfecting solutions on young and old e. faecalis biofilms in dentin canals using a novel dentin infection model and confocal laser scanning microscopy (clsm). | 2012 | 22980181 |