Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [kaposi sarcoma. t4 lymphocytes secrete growth factor]. | 1989 | 2565282 | |
| the effect of yohimbine on plasma levels of t3, t4 and cortisol in xylazine-immobilized white-tailed deer. | 1. the effect of yohimbine (y) on blood levels of thyroxine (t4), triiodothyronine (t3), and cortisol was investigated in 5 mature male white-tailed deer immobilized with xylazine hydrochloride (x). 2. t4 levels were erratic in x-treated deer, but stabilized in the x- and y-treated deer. 3. t3 remained unchanged in both groups. 4. cortisol levels have increased in x-treated deer, but declined in x- and y-treated deer. 5. yohimbine is a potent and safe antidote of x not affecting t3 and t4. cauti ... | 1989 | 2565187 |
| cardiac responses to the microinjections of excitatory amino acids into the intermediolateral cell column of the rat spinal cord. | sympathoexcitatory cardiovascular responses to the microinjections of l-glutamate into the intermediolateral cell column (iml) of the upper thoracic cord (c8 to t4) were studied. mean arterial pressure (map), heart rate (hr), the rate of increase in the left ventricular pressure (dp/dt) and contractility index were monitored in immobilized and artificially ventilated male wistar rats anesthetized with pentobarbital or isoflurane. on the right side, microinjections (10-20 nl) of l-glutamate (0.9- ... | 1989 | 2565136 |
| t cell regulation of human b cell proliferation and differentiation. regulatory influences of cd45r+ and cd45r- t4 cell subsets. | the immunoregulatory functions of human t4 cell subpopulations defined by mab to the cd45r molecule (2h4) were examined. both cd45r- and cd45r+ t4 cells that had been treated with mitomycin c (cd45r- and cd45r+ t4-mito) provided help for the generation of ig-secreting cells (isc) in cultures stimulated by pwm or by immobilized mab to cd3 (64.1). il-2 enhanced the generation of isc in pwm-stimulated cultures and in anti-cd3-stimulated cultures containing cd45r+ t4-mito. the generation of isc was ... | 1989 | 2564858 |
| train-of-four fade during onset and recovery of neuromuscular block: a study in non-anaesthetized subjects. | the actions of alcuronium, vecuronium and tubocurarine have been studied in the isolated forearms of six healthy, non-anaesthetized volunteers. the responses of adductor pollicis were measured during onset and recovery of neuromuscular block for each agent. there was a drug-related disparity between mechanomyogram (mmg) and electromyogram (emg) measurement of the first response of the train-of-four (t1) and of the ratio of the fourth (t4) to the first response (tof ratio). there were significant ... | 1989 | 2564783 |
| 1 alpha,25-dihydroxyvitamin d3 receptor distribution and effects in subpopulations of normal human t lymphocytes. | receptors for 1 alpha,25-dihydroxyvitamin d3 [1,25-(oh)2d3] are expressed upon activation of human lymphocytes, and the hormone inhibits in vitro the proliferation of mitogen-activated lymphocytes. in this study we examined the distribution of the 1,25-(oh)2d3 receptor protein in the two major subsets of t lymphocytes (t helper and t suppressor cells) and the effect of the hormone on their respective rates of proliferation. we activated normal lymphocytes in the presence of monocytes with phytoh ... | 1989 | 2564005 |
| glucoregulatory function of thyroid hormones: role of pancreatic hormones. | glucose metabolism was investigated in humans before and 14 days after 300 micrograms l-thyroxine (t4)/day using a sequential clamp protocol during short-term somatostatin infusion (500 micrograms/h, 0-6 h) at euglycemia (0-2.5 h), at 165 mg/dl (2.5-6 h), and during insulin infusion (1.0 mu.kg-1.min-1, 4.5-6 h). t4 treatment increased plasma t4 (+96%) and 3,5,3'-triiodothyronine (t3, +50%), energy expenditure (+8%), glucose turnover (+32%), and glucose oxidation (glucox +87%) but decreased thyro ... | 1989 | 2563199 |
| immunopathogenic mechanisms of hiv infection. | the acquired immunodeficiency syndrome (aids) follows infection with the human immunodeficiency virus (hiv) after a long and variable period of time. although hiv can be rapidly cytopathic in vitro for t4 cells, during early in vivo infection, t4 cell numbers are normal and few infected t4 cells can be found. there is increasing evidence that cells of the monocyte/macrophage lineage, including bone marrow precursor cells, can be infected by hiv and are relatively resistant to the cytopathic effe ... | 1989 | 2562941 |
| major depressive disorder and immunity. role of age, sex, severity, and hospitalization. | an association between depression and altered immunity has been suggested but has not been consistently demonstrated. we have studied 91 patients with unipolar major depressive disorder, and no mean differences were found between the patients and concurrently studied matched controls in mitogen-induced lymphocyte proliferation, lymphocyte subsets, and natural killer cell activity. there were, however, significant age-related differences between the depressed patients and controls in mitogen resp ... | 1989 | 2562915 |
| myod and achaete-scute: 4-5 amino acids distinguishes myogenesis from neurogenesis. | the myogenic determination gene myod contains a 60 amino acid domain that is necessary for both sequence-specific dna binding and myogenic conversion of transfected c3h10t1/2 mouse embryo fibroblasts. we have generated deletion, insertion, and substitution mutants to probe the structure and function of this region, both in vitro and in vivo. our results are consistent with a previous proposal that a helix-loop-helix (hlh) motif mediates protein dimerization. a highly basic region located immedia ... | 1989 | 2562185 |
| the effect of thyroid hormones on the membrane adenylate cyclase activity in sickle red blood cells in vitro. | a comparative study of thyroid hormone responsive membrane adenylate cyclase activity has been carried out on normal (hb-aa) and sickle cell (hb-ss) subjects. thyroid hormones (t3 and t4) and selected analogues (triac and dlt) enhanced adenylate cyclase activity in hb-aa membranes. the interaction of hb-ss membranes with the hormones and analogues was not significant except for t3 which moderately stimulated hb-ss membrane adenylate cyclase. we suggest that circulating irreversibly sickled cells ... | 1989 | 2561120 |
| amino acid similarities to other proteins offer insights into roles of umud and umuc in mutagenesis. | the products of the umud and umuc genes are required for most uv and chemical mutagenesis in escherichia coli. the genes are organized in an operon that is repressed by lexa and regulated as part of the sos response. the umud protein shares homology with the carboxyl-terminal domain of lexa. genetic evidence now indicates that reca-mediated cleavage activates umud for its role in mutagenesis. the cooh-terminal fragment of umud is both necessary and sufficient for this role. similarities of umud ... | 1989 | 2561111 |
| a simple and versatile method for the preparation of vector-primers by adapter-end-primer ligation. | a group of efficient cdna cloning strategies employs vector-primers where cdna synthesis starts from the oligo(dt)-primer tail, which is conventionally attached to cloning vectors by use of terminal deoxynucleotidyl transferase. an alternative, efficient and more versatile method of vector-primer preparation is to directly ligate, by use of t4 dna ligase, a double-digested vector, e.g., ptz18r/pst i/bam hi, to a synthetic (bam hi)-adapter-end-primer, 5'-pgatcc-tn or 5'-pgatcc-site-specific seque ... | 1989 | 2561065 |
| circadian rhythm parameters of endocrine functions in elderly subjects during the seventh to the ninth decade of life. | the circadian rhythm of 17 endocrine parameters (acth, aldosterone, cortisol, c-peptide, dhea-s, fsh, growth hormone, insulin, lh, 17-oh progesterone, prolactin, testosterone, total t3, total t4 and tsh and estradiol and progesterone in women only) were studied in 63 clinically apparently healthy men (124 profiles) and 86 women (154 profiles) during the 7th to 9th decade of life. the subjects lived under very uniform conditions in a home for the aged with their daily schedule standardized by ins ... | 1989 | 2560701 |
| a model for the complex between the helix destabilizing protein gp32 of bacteriophage t4 and single-stranded dna. | a model for the structure of the complex between the helix-destabilizing protein of bacteriophage t4, gp32, and single-stranded dna is proposed. in this model the bases are arranged in a helix, that is characterized by a relatively large distance between successive bases, a substantial base tilt, in combination with a small rotation per base. this helix is further organized into a tertiary structure, possibly a superhelix, of which the corresponding protein shell corresponds to the relatively ri ... | 1989 | 2559746 |
| observations on blood leucocytes and lymphocyte subsets in sheep infected with bovine leukaemia virus: a progressive study. | haematological parameters and reactivity of lymphocyte antigens to monoclonal antibodies were studied over a 10-month period in sheep experimentally infected with bovine leukaemia virus (blv). blv-inoculated animals seroconverted within 1 month and showed a significant lymphocytosis 2-6 weeks after infection. control animals inoculated with blv-free lymphocytes showed a stronger and more immediate neutrophil response than those inoculated with blv-positive lymphocytes. one month after infection, ... | 1989 | 2559536 |
| creatine kinase-bb activity in malignant tumors and in sera from patients with malignant diseases. | creatine kinase (ck ec 2.7.3.2) and ck-bb activity was analyzed in 41 malignant tumors of 6 different sites and different histological structures. the same analyses were done on 150 sera of patients with malignant diseases of various localizations. the rate of ck activity was determined kinetically, whereas tissue and serum ck-bb were separated chromatographically (mercer). insofar as malignant tumor tissues are concerned, the highest average rate of ck-bb activity was detected in tumors of the ... | 1989 | 2559523 |
| [the advantages of reprimum therapy in pulmonary sarcoidosis and other granulomatous diseases]. | in sarcoidosis and other granulomatous non-caseous diseases, the election treatment is immunosuppressive, mainly with cortisones that ensure more than 70% lasting remissions. continuous use of cortisones for a long time (8-30 months) in high doses leads to serious side effects: gastric and intestinal ulcers, obesity, osteoporosis, suprarenal dysfunction, sensitivity to infections. good results and elimination of the important side effects were obtained by treatment with reprimum--a semisynthetic ... | 1989 | 2559463 |
| [use of non-traditional methods of examination in the differential diagnosis of cancer and tuberculosis of the lungs]. | the immunoradiometric assay of the blood serum was performed in 61 patient with cancer and in 57 patients with tuberculosis of the lungs to determine the content of adrenocorticotropic hormone (acth), hydrocortisone, triiodothyronine++ (t3), thyroxin (t4), ferritin and carcinoembryonic antigen (cea). in 200 patients (104 suffered from pulmonary tuberculosis, 9--pulmonary cancer), entropy of the protein fractions of the blood serum was determined. it was established that in differential diagnosis ... | 1989 | 2559239 |
| substrate properties of 25-nt parallel-stranded linear dna duplexes. | four 25-nt oligonucleotides consisting of sequences of da and dt (d1-4) have been synthesized. as shown in a companion paper (rippe et al., 1989), the two combinations d1.d3 and d2.d4 form normal antiparallel duplexes, whereas the pairs d1.d2 and d3.d4 constitute duplexes with the same sequences, but with the two strands parallel to each other. the activities of the following dna processing enzymes and chemical reagents on the parallel stranded (ps) and antiparallel stranded (aps) duplexes were ... | 1989 | 2558723 |
| comparison of cooperative and isolated site binding of t4 gene 32 protein to ssdna by 1h nmr. | deuteriation of all aromatic protons of gene 32 protein (g32p) from phage t4, followed by selective introduction of specific protons, has allowed the precise identification of the number and magnitude of the chemical shift changes induced in the aromatic protons when g32p binds noncooperatively or cooperatively to nucleotides. signals from five tyr residues are shifted by binding of g32p to d(pa)8 or d(pa)40-60; however, the change from noncooperative, d(pa)8, to cooperative, d(pa)40-60, binding ... | 1989 | 2557909 |
| energy expenditure associated with sodium/potassium transport and protein synthesis in skeletal muscle and isolated hepatocytes from hyperthyroid sheep. | the object of the present study was to determine the effect of thyroxine (t4) treatment of sheep on protein synthesis and associated energy costs in skeletal muscle and hepatocytes. protein synthesis, and ouabain-sensitive and cycloheximide-sensitive respiration in isolated intercostal muscle and hepatocytes were determined in sheep after 5 weeks of daily injections of either saline or t4. plasma t4 and total triiodothyronine (t3) concentrations were doubled and free t3 concentrations were quadr ... | 1989 | 2557888 |
| superhelical stress restrained in plasmid dna during repair synthesis initiated by the uvra, b and c proteins in vitro. | purified uvra, uvrb, uvrc, uvrd, pola and lig proteins from escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid dna in an in vitro test system. the analysis of labeled reaction products on specific gel systems suggests that the uvr excinuclease has the ability to restrain the superhelical stress in the template dna during the repair process. this feature, observed in the case of the uvr system is not foun ... | 1989 | 2557590 |
| physical structure and molecular cloning of equine cytomegalovirus dna. | restriction endonuclease (re) mapping studies and molecular hybridization analyses were conducted to determine the molecular structure of the genome of equine cytomegalovirus (ecmv). the ecmv genome is a linear, double-stranded dna with a molecular size of 126 +/- 0.6 mda (189 kbp). a library of cloned bamhi, ecori, and hindiii fragments of the viral genome was used to construct re maps. individual 32p-labeled cloned dna fragments were hybridized to southern blots of viral genomic dna digested t ... | 1989 | 2556843 |
| cruciform cutting endonucleases from saccharomyces cerevisiae and phage t4 show conserved reactions with branched dnas. | we have purified a cruciform dna resolving endonuclease (endo x3) greater than 1000-fold from crude extracts of mitotically growing saccharomyces cerevisiae. the enzyme shows high specificity for dnas with secondary structures and introduces characteristic patterns of staggered 'nicks' in the immediate vicinity of the structure. the following substrates were analyzed in detail: (i) naturally occurring four-way x junctions in cruciform dna of a supercoiled plasmid; (ii) synthetic four-way x junct ... | 1989 | 2556268 |
| the essential 65-kilodalton dna-binding protein of herpes simplex virus stimulates the virus-encoded dna polymerase. | the 65-kilodalton dna-binding protein (65kdbp) of herpes simplex virus type 1 (hsv-1), the product of the ul42 gene, is required for dna replication both in vitro and in vivo, yet its actual function is unknown. by two independent methods, it was shown that the 65kdbp stimulates the activity of the hsv-1-encoded dna polymerase (pol). when pol, purified from hsv-1-infected cells, was separated from the 65kdbp, much of its activity was lost. however, addition of the 65kdbp, purified from infected ... | 1989 | 2555539 |
| identification of a 27-kda protein with the properties of type ii iodothyronine 5'-deiodinase in dibutyryl cyclic amp-stimulated glial cells. | type ii iodothyronine 5'-deiodinase (5'd-ii) catalyzes the intracellular conversion of thyroxine (t4) to 3,5,3'-triiodothyronine (t3), producing greater than 90% of the bioactive thyroid hormone in the cerebral cortex. in cultured glial cells, expression of this enzyme is camp dependent. exploiting the camp-dependent nature of this enzyme in these cells and utilizing n-bromoacetyl-l-3'- or 5'-[125i]thyroxine (brac[125i]t4) to affinity label cellular proteins, a 27-kda protein with the properties ... | 1989 | 2555364 |
| a site-specific endonuclease and co-conversion of flanking exons associated with the mobile td intron of phage t4. | the product of the td intron open reading frame (orf) of phage t4 is required for high-frequency transfer of the intervening sequence from intron-plus (in+) to intron-minus (in-) alleles. in vivo studies have demonstrated that the td orf product targets cleavage of td in- dna, and that cleavage is correlated with intron inheritance [quirk et al., cell 56 (1989) 455-465]. in the present study we show by in vitro synthesis of the td intron orf product, that the protein possesses endonuclease activ ... | 1989 | 2555262 |
| thyrotropic activity of the ovine corticotropin-releasing factor in the chick embryo. | an injection of several doses of ovine corticotropin-releasing factor (ocrf) was made into an allantoic blood vessel of 18-day-old chick embryos. all doses used (0.5, 1, 2, and 5 micrograms) induce a quick increase of plasma corticosterone concentrations after 15 and 30 min and 1 and 2 hr and this in a dose-dependent manner with the high doses having a prolonged effect lasting up to 2 hr. an increase in plasma levels of thyroxine (t4) and triiodothyronine (t3) was observed after 1 and 2 hr. afte ... | 1989 | 2555247 |
| t-lymphocyte subsets in gut and blood of liver transplant recipients with and without cytomegalovirus gastroenteritis. | the effects of orthotopic liver transplantation (oltx) and cytomegalovirus (cmv) gastroenteritis on the type of mononuclear cells within the upper gastrointestinal tract were determined. nineteen liver transplant recipients were studied both before and after transplantation. each underwent a pan-upper gastrointestinal endoscopy with biopsy of the antrum and duodenum before and four weeks following liver transplantation. a panel of monoclonal antibodies prepared against hla-dr, nk, il-2r, t11, t4 ... | 1989 | 2555122 |
| the radiology of the right superior intercostal vein. | the right superior intercostal vein is visualised on ct examination as a circular opacity laterally at the right aspect of the vertebral body at the t4-t5 level. in venographic examination the rsiv appears to be formed by the confluence of the venous channels, the right second, third and fourth intercostal veins. in superior vena cava obstruction the rsiv is an important collateral pathway: the flow through the vein depends on the site of the obstruction, anterograde if the obstruction is superi ... | 1989 | 2554413 |
| changes in multiple opioid receptors in regions of the brain in rats treated chronically with thyroxine. | the effect of the administration of thyroxine (t4) (1 mg/kg, s.c.), to male sprague-dawley rats on alternate days for 18 days, on the binding of opioid ligands, [3h]tyr-d-ala-gly-mephe-gly-ol (dago, mu receptors), [3h]tyr-d-ser-gly-phe-leu-thr (dstle, delta receptors) and [3h]ethylketocyclazocine (ekc, kappa receptors) to membranes of regions of the brain was determined. the chronic administration of thyroxine to rats decreased their rate of gain of body weight, increased colonic temperature and ... | 1989 | 2554187 |
| differential hormone-binding characteristics of thyroxine-binding globulin in hepatocellular carcinoma and cirrhosis. | to determine whether previous observations suggesting reduced ability of thyroxine-binding globulin (tbg) to bind exogenous thyroid hormones in hepatocellular carcinoma (hcc) might form the basis of a useful clinical test, we have investigated a larger series involving 49 patients and 10 healthy subjects. the binding ratio (mol hormone bound/mol tbg at a given hormone concentration) for both t4 and t3 was significantly reduced when compared to both cirrhotic and normal subjects. no overlap in t4 ... | 1989 | 2553799 |
| molecular epidemiologic analysis of the type a streptococcal exotoxin (erythrogenic toxin) gene (spea) in clinical streptococcus pyogenes strains. | a molecular epidemiology analysis was performed with over 440 clinical isolates of streptococcus pyogenes obtained from 11 different countries in order to determine the frequency of occurrence of the type a streptococcal exotoxin (erythrogenic toxin) gene (spea) among group a strains. the colony hybridization technique employing a specific internal fragment of the spea gene was used for initial screening, and all positive results were further confirmed by the southern hybridization technique. am ... | 1989 | 2553612 |
| thyroid hormone action on acth secretion. | thyroid hormone effects on pituitary acth have not been well established. adult male sprague-dawley rats were rendered hypo- and hyperthyroid while undergoing treatment with 6-propylthiouracil (ptu) and l-thyroxine (t4). at the time of decapitation, plasma values for t4 (micrograms/100 ml) were 3.9 +/- 0.4 in the control, 17.3 +/- 2.2 in the t4 and less than 2 in the ptu treated group; plasma t3 and tsh confirmed hyper- and hypothyroidism in the t4 and ptu treated groups respectively. plasma imm ... | 1989 | 2553572 |
| antigen-stimulated human interferon-gamma generation: role of accessory cells and their expressed or secreted products. | in response to cytomegalovirus (cmv) and toxoplasma gondii antigens, t4+ cells from seropositive donors produce interferon-gamma (ifn-gamma) by different mechanisms; one (t. gondii) dependent upon and the other (cmv) largely independent of interleukin-2 (il-2) and its receptor. to determine whether ifn-gamma-generating mechanisms unrelated to il-2 also differ, we examined the requirement for accessory cells and their expressed or secreted products. in response to both specific antigens, ifn-gamm ... | 1989 | 2553309 |
| [role of sex dimorphism in the pharmacological action of etimizol]. | it has been shown that in female rats the level of acth, corticosterone in the blood, relative mass of adrenals, maintenance of t3, t4 in the serum and liver was significantly higher, but the activity of liver enzyme microsomes system was lower than in males; no sex differences were observed in myocardial creatine phosphokinase system. the influence of the etimizol on the female rats significantly speed up amidopyrine n-demethylation and biotransformation of hexobarbital. in males these systems ... | 1989 | 2553148 |
| modulation of the neonatal pituitary and adrenocortical responses to stress by thyroid hormones in the rat: effects of hypothyroidism and hyperthyroidism. | neonatal rats exhibit a period of diminished pituitary and adrenocortical responses to stress during the first 2 weeks of life. since thyroid hormones are known to affect brain development, modulation of these responses to stress by alterations in thyroid hormone status have been investigated in hypothyroid (hypo) and hyperthyroid (hyper) rat pups. changes in acth and corticosterone (b) levels were measured under basal and stress conditions (3 min exposure to ether vapors) in neonates of various ... | 1989 | 2552340 |
| stimulation of the chlorinating activity of human myeloperoxidase by thyroid hormones and analogues. | thyroxine and triiodothyronine concentrations of 50 nm or lower can stimulate the chlorinating activity of the myeloperoxidase-h2o2-cl- antimicrobial system in vitro. the initial rates of the chlorinating reaction with monochlorodimedone were similar for both thyroid hormones. maximum stimulation occurred around ph 6 and a linear relationship exists between stimulation and thyroxine concentrations up to at least 1 microm. of the various thyroxine analogues tested, stimulation was in the order: t ... | 1989 | 2551809 |
| characterization of tumor necrosis factor-alpha receptors in human and rat thyroid cells and regulation of the receptors by thyrotropin. | administration of recombinant human tumor necrosis factor-alpha (tnf) to rats and mice produces a model of nonthyroid illness in which there is impairment of hypothalamic-pituitary thyroid function, including reduced serum concentrations of t4 and t3, reduced thyroid radioiodine uptake, and reduced response to tsh. in this study, we tested the binding and effects of tnf on frtl-5 cells and on four human thyroid carcinoma cell lines. the tsh-stimulated [125i]iodide uptake by frtl-5 cells was inhi ... | 1989 | 2551628 |
| harderian glands of golden hamsters: morphological and biochemical responses to thyroid hormones. | manipulation of circulating levels of thyroid hormones modifies harderian gland structure and porphyrin concentrations in male and female golden hamsters. specifically, thyroxine (t4) and triiodothyronine (t3) induce the morphological conversion of the harderian glands of females to approximate those of the male. further, porphyrin concentrations are markedly decreased by this treatment. this effect occurs in ovariectomized animals as well, indicating that the gonads are not involved. suppressio ... | 1989 | 2550527 |
| thyroid-stimulating activity in sera of normal pregnant women. | to evaluate the effect of a thyroid stimulator on thyroid function in the sera of normal pregnant women, we measured thyroid-stimulating activity (tsa) using a highly sensitive bioassay based on camp accumulation in cultured rat frtl-5 thyroid cells. serum was pretreated with 10% polyethylene glycol (peg), and the supernatant (peg-pretreated serum) was then used in the following studies. frtl-5 cells were preincubated in 5h medium and incubated for 2 h with peg pretreated serum, and camp was mea ... | 1989 | 2550510 |
| effects of epidermal growth factor on thyroglobulin and adenosine 3',5'-monophosphate production by cultured human thyrocytes. | while several workers have identified epidermal growth factor (egf) receptors on human thyroid membranes, very few reports have described egf effects on intact human thyroid cells in primary culture, and these were short term studies indicating that egf effects were primarily inhibitory [reduced iodide uptake and thyroglobulin (tg), t4, and t3 release]. paradoxically, in vivo egf stimulates thyroid growth and increases colloid stores. in this study we examined the effects of egf on cultured thyr ... | 1989 | 2550506 |
| the phage t4 uvs y recombination protein stabilizes presynaptic filaments. | the bacteriophage t4 uvsy protein is required for efficient recombination in t4-infected escherichia coli cells. previous in vitro work has shown that the purified uvsy protein is an accessory protein; it stimulates homologous pairing catalyzed by the phage uvsx protein (a reca-like recombinase) under certain conditions. we show here that this effect can be traced, at least in part, to a uvsy-dependent stabilization of uvsx protein-single-stranded dna complexes. these presynaptic filaments are o ... | 1989 | 2550444 |
| characterization of four herbicide-resistant mutants of rhodopseudomonas viridis by genetic analysis, electron paramagnetic resonance, and optical spectroscopy. | herbicides of the triazine class block electron transfer in the photosynthetic reaction centers of purple bacteria and psii of higher plants. they are thought to act by competing with one of the electron acceptors, the secondary quinone, qb, for its binding site. several mutants of the purple bacterium rhodopseudomonas viridis resistant to terbutryn [2-(methylthio)-4-(ethylamino)-6-(tert-butylamino)-s-triazine] have been isolated by their ability to grow photosynthetically in the presence of the ... | 1989 | 2550055 |
| [an autopsy case of aids with disseminated cytomegalovirus infection and neurological disturbance]. | we report a case of acquired immunodeficiency syndrome (aids) complicated by disseminated cmv infection and neurological disturbance. a 21 years old male with hemophilia a was diagnosed as having aids in feb. 1986 because of interstitial pneumonia and esophageal candidiasis. since jan. 1987 he had complained of hypesthesia in the legs. on mar. 14 he was admitted due to diarrhea. the laboratory data revealed that wbc was 4,000/microliters including 29% of lymphocytes, 1.6% of okt4+-, 71.6% of okt ... | 1989 | 2549283 |
| using transposon tn5 insertions to sequence bacteriophage t4 gene 11. | a simple and rapid method of creating an overlapping set of deletions in cloned dna in preparation for sequencing has been developed. the method is based on a positive selection for tn5 transposition into the cloned dna fragment on a high-copy-number filamid, resolution of potential filamid dimers by filamentous phage infection, and the use of tn5 both as a "portable" restriction enzyme site for in vitro dna deletion and a "portable" sequencing primer binding site to initiate dna sequencing reac ... | 1989 | 2548819 |
| ring-opened 7-methylguanine nucleotides are resistant to nuclease p1 digestion and good substrates to polynucleotide kinase. | dimethyl sulfate was used to prepare 7-methyl-2'-deoxy-guanosine 3'-monophosphate (7-methyl-dgmp), which was ring-opened in alkali to 2'-deoxy-n5-methyl-n5-formyl-2,5,6-triamino-4-oxopyrimidine 3'-monophosphate (rom-dgmp). rom-dgmp was not dephosphorylated by nuclease p1 in contrast to normal deoxynucleotides. it was efficiently 5'-phosphorylated by t4 polynucleotide kinase. when methylated dna was alkali-treated and digested with micrococcal nuclease, spleen phosphodiesterase and nuclease p1, r ... | 1989 | 2548753 |
| cns cell groups regulating the sympathetic outflow to adrenal gland as revealed by transneuronal cell body labeling with pseudorabies virus. | the cns cell groups that innervate the sympathoadrenal preganglionic neurons of rats were identified by a transneuronal viral cell body labeling technique combined with neurotransmitter immunohistochemistry. pseudorabies virus was injected into the adrenal gland. this resulted in retrograde viral infections of the ipsilateral sympathetic preganglionic neurons (t4-t13) and caused retrograde transneuronal cell body infections in 5 areas of the brain: the caudal raphe nuclei, ventromedial medulla, ... | 1989 | 2548665 |
| comparative x-ray crystallographic evidence for a beta-bend conformation as the active structure for peptide t in t4 receptor recognition. | a sequence similarity has been found between two segments of endothiapepsin (acid proteinase, 2ape), bovine pancreatic ribonuclease a, and peptide t, a segment of the gp120 protein of human immune deficiency virus (hiv), which has been implicated in blocking viral attachment to the t4 receptor. the two similar sequences of the acid proteinase enzyme are leu-ile-asp-ser-ser-ala-tyr-thr (residues 169-176) and tyr-thr-gly-ser-leu-asn-tyr-thr (residues 175-182). since the x-ray crystallographic stru ... | 1989 | 2548525 |
| tissue-specific isoform regulation of na+-k+-atpase by thyroid hormone in ferrets. | we have shown previously that in the ferret heart there are two isoforms of na+-k+-atpase, alpha(+) and alpha, and that these isoforms undergo developmental changes. in the present study, we examine regulation of the isoenzymes by thyroid hormone, which is well known to increase activity of na+-k+-atpase in different tissue preparations. ferrets were injected with l-thyroxine (t4) (0.5 mg/kg, sc) for 3 wk. the t4-treated ferrets gained 58 +/- 32 g body wt compared with 366 +/- 24 g for the contr ... | 1989 | 2548405 |
| [the clinical value of computed tomography in the pretherapeutic t-staging of orofacial tumors]. | the clinical role of contrast-enhanced ct for pre-operative t-staging of oro-facial tumours was evaluated in 174 patients. during stages t1 and t2, clinical examination is 90% accurate and has a significantly higher sensitivity than ct, with an accuracy of only 53%. the clinical diagnosis of t1 and t2 is, however, made with undue frequency; ct makes it possible to obtain correct staging. combination of ct and clinical examination can improve correct staging from 46% to 81%. during stages t3 and ... | 1989 | 2548237 |
| two early genes of bacteriophage t5 encode proteins containing an ntp-binding sequence motif and probably involved in dna replication, recombination and repair. | it is demonstrated, by computer-assisted analysis, that t5 bacteriophage early genes d10 and d13 encode proteins containing the purine ntp-binding sequence motif. the d10 gene product is shown to be a member of a recently characterized superfamily of (putative) dna and rna helicases. the d13 gene product is related at a statistically significant level, to the gene 46 product of bacteriophage t4 which is a component of an exonuclease involved in phage dna replication, recombination and repair. a ... | 1989 | 2547651 |
| common sites for recombination and cleavage mediated by bacteriophage t4 dna topoisomerase in vitro. | we have previously shown that purified t4 dna topoisomerase promotes illegitimate recombination between two lambda dna molecules, or between lambda and plasmid dna in vitro (ikeda, h. (1986) proc. natl. acad. sci. u. s. a. 83, 922-926). since the recombinant dna contains a duplication or deletion, it is inferred that the cross-overs take place between nonhomologous sequences of lambda dna. in this paper, we have examined the sequences of the recombination junctions produced by the recombination ... | 1989 | 2546934 |
| etiology of childhood hypothyroidism. | this study prospective without any selection bias included 80 of the 152 hypothyroid infants and children seen over the past six years. the clinical diagnosis was confirmed by tsh and thyroid hormone (t3, t4) studies. scanning for thyroid with tc99m pertechnetate was carried out in all except seven older children with grade ii and iii goiters where 131i uptake studies were done. serum thyroglobulin (ria) was estimated and antithyroglobulin and antimicrosomal antibodies were tested. based on thyr ... | 1989 | 2546895 |
| [laryngeal and hypopharyngeal carcinoma--the limits and advantages of sonography]. | 29 patients with carcinoma of the larynx and the hypopharynx were evaluated not only for staging cervical lymph nodes but also for classification of the primary tumour. with appropriate technique it is possible to assess tumour infiltration (t4) of surrounding tissue of the larynx at all levels. tumour classification t1 to t3 is possible in patients with tumour of the epiglottis (regio supraglottis, uicc). the endolaryngeal tumour t1 to t3 (regio glottis and subglottis, uicc) still remains the d ... | 1989 | 2546206 |
| complications of hypogastric artery cisplatin infusions. | in an attempt to increase the therapeutic index of cisplatin (cddp), 29 continuous 48 hour intra-arterial (ia) infusions of 100-150 mg/m2 cddp were given to 26 patients with bulky stage t3-t4 bladder cancer. hypogastric artery catheters were placed distal (n = 42) or proximal (n = 7) to the origin of the superior gluteal artery. atherosclerosis (n = 5) or aneurysm (n = 2) prevented successful ia catheter placement. catheter maintenance resulted in no bleeding or thromboembolic episodes. urethral ... | 1989 | 2545974 |
| the bacteriophage t4 dna replication fork. only dna helicase is required for leading strand dna synthesis by the dna polymerase holoenzyme. | seven bacteriophage t4-encoded proteins reconstitute a dna replication apparatus that catalyzes coupled leading and lagging strand dna synthesis at a replication fork in vitro. the proteins involved are the t4 dna polymerase holoenzyme (the products of t4 genes 43, 44/62, and 45), a helix-destabilizing (ssb) protein (gene 32 protein), and the t4 primosome which is composed of a dna helicase (gene 41 protein) and a primase (gene 61 protein). we show here that the presence of 41 protein on the lag ... | 1989 | 2545703 |
| acute myeloid leukemia, fab m-1 microgranular variant: a multiparameter study. | acute leukemia was diagnosed in 62 adults and children over a recent 13-month period. using light microscopy, cytochemical profiles, surface markers, and cytogenetics, 25 cases were classified as acute myeloid leukemia (aml) and 32 as acute lymphoblastic leukemia (all). the remaining 5 cases of de novo acute leukemia were unclassifiable. the routine cytochemical battery used on these 62 cases included: myeloperoxidase, sudan black b, nonspecific esterase, and periodic acid-schiff (pas). flow mar ... | 1989 | 2545666 |
| cytomegalovirus enhances lysis of hiv-infected t lymphoblasts. | a t4+ lymphoblastoid cell line (cr-10) persistently infected with the human immunodeficiency virus (hiv) and designated cr-10/nit was superinfected with cytomegalovirus (cmv) isolated from peripheral blood mononuclear cells of a patient with aids. a productive cmv cycle in the cr-10/nit lymphoblasts was demonstrated by fluorescent antibody staining (if) using a monoclonal antibody (mab) to the 150-kda major capsid protein, by infectivity assays and by electron microscopy (em). two-color if analy ... | 1989 | 2545634 |
| conformation and dynamics of a left-handed z-dna hairpin: studies of d(cgcgcgttttcgcgcg) in solution. | the physical properties of the dna oligomer d(cgcgcgttttcgcgcg) in solvents containing 4 m naclo4 and 0.1 m nacl were investigated by proton nmr, optical melting, and circular dichroism spectroscopy. results of these investigations are as follows: (i) the dna hexadecamer exists as a unimolecular hairpin in either high or low salt. (ii) in high salt the stem region of the hairpin is in the left-handed z conformation. (iii) in either high or low salt, the duplex stem of the hairpin is stabilized a ... | 1989 | 2545256 |
| the effect of thyroxine, 3, 5-dimethyl-3'isopropyl-l-thyronine and iodized oil on fetal brain development in the iodine-deficient sheep. | studies have been carried out to investigate the role of maternal and fetal thyroid function in the effects of iodine deficiency on fetal brain development in sheep. iodine deficiency was established with an especially prepared low-iodine diet of maize and pea pollard. the iodine-deficient sheep were mated and the end of the second trimester of pregnancy (100 days gestation) were divided into groups which received either a sc injection of t4 or 3,5-dimethyl-3-isopropyl-l-thyromine or an injectio ... | 1989 | 2545065 |
| impaired expression of certain prereplicative bacteriophage t4 genes explains impaired t4 dna synthesis in escherichia coli rho (nusd) mutants. | the escherichia coli rho 026 mutation that alters the transcription termination protein rho prevents growth of wild-type bacteriophage t4. among the consequences of this mutation are delayed and reduced t4 dna replication. we show that these defects can be explained by defective synthesis of certain t4 replication-recombination proteins. expression of t4 gene 41 (dna helicase/primase) is drastically reduced, and expression of t4 genes 43 (dna polymerase), 30 (dna ligase), 46 (recombination nucle ... | 1989 | 2544560 |
| a new sensitive 32p-postlabeling assay based on the specific enzymatic conversion of bulky dna lesions to radiolabeled dinucleotides and nucleoside 5'-monophosphates. | a new sensitive 32p-postlabeling assay for dna adducts has been developed in which dna is hydrolyzed initially by nuclease p1 and prostatic acid phosphatase instead of micrococcal nuclease and spleen phosphodiesterase as employed in previous postlabeling procedures. when dna containing bulky adducts, x1, x2, .....xn, is digested with nuclease p1 at ph 5, normal nucleotides are released as 5'-monophosphates, pn, while adducts are excised as 5'-phosphorylated dinucleotides, pxipn, because internuc ... | 1989 | 2544310 |
| evidence that the intron open reading frame of the phage t4 td gene encodes a specific endonuclease. | the phage t4 thymidylate synthase (td) gene contains an intron open reading frame that encodes a 245-amino acid-long basic protein (chu, f. k., maley, g. f., west, d. k., belfort, m., and maley, f. (1986) cell 45, 157-166). the open reading frame (irf) has been cloned as a fusion protein behind a phage t7 promoter and overexpressed in escherichia coli. the amplified irf protein is associated with insoluble inclusion bodies and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis ... | 1989 | 2543665 |
| subcellular distribution of a nitroxide spin-labeled netropsin in living kb cells. electron paramagnetic resonance and sequence specificity studies. | a nitroxide spin-labeled netropsin was studied by epr spectroscopy with respect to its uptake and localization in living kb cells. whereas the drug was taken up readily, there was relatively little drug in the cytoplasm, but a significant concentration of the drug in the cell nucleus. the epr signal in the latter site corresponded to a relatively freely rotating radical. the drug exhibited good intracellular stability up to 25 hr. while a delta tm of 24 degrees between the spin-labeled netropsin ... | 1989 | 2543417 |
| evidence for thyroxine transport by the lung and heart capillary endothelium. | the uptake and transport of carrier-bound thyroxine by the endothelium were investigated by perfusing through the heart and lung of young rats radiolabeled thyroxine bound to prealbumin ([125i]t4pa) or serum ([125i]t4s). in addition these complexes were tagged to 5-nm gold particles to obtain quantitative (radioassay) and qualitative (autoradiography) data from the same experiment. the complexes (prewarmed at 37 degrees) were perfused in situ at various concentrations (1 to 50 muci/ml) for time ... | 1989 | 2542733 |
| dna substrate structural requirements for the exonuclease and polymerase activities of procaryotic and phage dna polymerases. | a dna duplex covalently cross-linked between specific bases has been prepared. this and similar duplexes are substrates for the polymerase and exonuclease activities of the klenow fragment of escherichia coli dna polymerase i and t4 and t7 dna polymerases. the action of klenow fragment on these duplexes indicates that the polymerase site does not require that the dna duplex undergo strand separation for activity, whereas the exonuclease site requires that at least four base pairs of the primer s ... | 1989 | 2541768 |
| denv gene of bacteriophage t4 restores dna excision repair to mei-9 and mus201 mutants of drosophila melanogaster. | the denv gene of bacteriophage t4 was fused to a drosophila hsp70 (70-kda heat shock protein) promoter and introduced into the germ line of drosophila by p-element-mediated transformation. the protein product of that gene (endonuclease v) was detected in extracts of heat-shocked transformants with both enzymological and immunoblotting procedures. that protein restores both excision repair and uv resistance to mei-9 and mus201 mutants of this organism. these results reveal that the denv gene can ... | 1989 | 2541436 |
| [flow cytometric analysis of myeloperoxidase negative acute unclassifiable leukemias by monoclonal antibodies. acute myelogenous and acute megakaryocytic leukemia]. | pretreatment peripheral and/or bone marrow blasts from 14 patients with acute unclassifiable leukemia (aul) expressing myeloid related cell-surface antigen (cdii) or megakaryocyte-platelet related cell-surface antigen (okm6), were isolated for further analysis in this study. among 11 cases of cd11+aul, despite a lack of myeloperoxidase (mpo) activity, one patient's blasts possessed auer rod in a basophilic cytoplasm and another one's blasts expressed mpo maintaining the same surface phenotype af ... | 1989 | 2541276 |
| cell-mediated immune cardiocyte injury in viral myocarditis of mice and patients. | the cellular immune mechanism of cardiocyte injury in viral myocarditis was investigated by observing and analyzing the interactions among cardiocytes, t cells, b cells, natural killer (nk) cells and macrophages in situ in the myocardium of our murine model (c3h/he mice) and of human patients. in murine coxsackie b3 virus myocarditis, lymphocyte subsets were identified by light and electron microscopic immunohistochemical techniques with antibodies against specific antigens of pan t (thy 1.2), h ... | 1989 | 2541265 |
| opioids induce postural asymmetry in spinal rat: the side of the flexed limb depends upon the type of opioid agonist. | the kappa-agonists bremazocine and dynorphin(1-13), the sigma-agonist skf 10.047 as well as the delta-agonists [d-ala2,d-leu5]-enkephalin (dadl) and met-enkephalin, but not the mu-agonist morphine, applied subarachnoidally to the caudal portion of the transected spinal cord (at the t3-t4 level) induced postural asymmetry of the hind limbs in rats. asymmetry was registered visually. the leg was regarded as flexed if its projection on the longitudinal axis of the animal was smaller than that of it ... | 1989 | 2540878 |
| enzymatic analysis of isomeric trithymidylates containing ultraviolet light-induced cyclobutane pyrimidine dimers. ii. phosphorylation by phage t4 polynucleotide kinase. | phage t4 polynucleotide kinase (ec 2.7.1.78) proved incapable of catalyzing the phosphorylation of thymidylyl-(3'----5')-thymidine containing either a cis-syn-cyclobutane pyrimidine dimer (d-t less than p greater than t) or a 6-4'-[pyrimidin-2'-one]pyrimidine photoproduct (d-t[p]-t), and similarly the uv-modified compounds of (dt)3 bearing either photoproduct at their 5'-end (d-t less than p greater than tpt and d-t[p]tpt). in contrast, the 3'-structural isomers of these trinucleotides (d-tpt le ... | 1989 | 2539380 |
| interaction of bisantrene anti-cancer agents with dna: footprinting, structural requirements for dna unwinding, kinetics and mechanism of binding and correlation of structural and kinetic parameters with anti-cancer activity. | a topoisomerase i assay in conjunction with covalently closed circular dna afforded unwinding angles for a series of eight analogs of the anti-cancer agent bisantrene. values ranging from 33 degrees to zero comprised three classes wherein the extent of dna interaction correlates with anti-cancer activity. the binding of bisantrene compounds to t4 dna suggests at least partial interaction via the minor groove. mpe footprinting evaluation of bisantrene showed no evidence for selective protection o ... | 1989 | 2539168 |
| structure of the drosophila dna topoisomerase ii gene. nucleotide sequence and homology among topoisomerases ii. | we have determined the nucleotide sequence of the drosophila dna topoisomerase ii gene. data from primer extension and s1 nuclease protection experiments were combined with comparisons of genomic and cdna sequences to determine the structure of the mature messenger rna. this message has a large open reading frame of 4341 nucleotides. the length of the predicted protein is 1447 amino acids with a molecular weight of 164,424. topoisomerase ii can be divided into three domains: (1) an n-terminal re ... | 1989 | 2538621 |
| role of n-linked glycans in the interaction between the envelope glycoprotein of human immunodeficiency virus and its cd4 cellular receptor. structural enzymatic analysis. | gp120 and cd4 are two glycoproteins that are considered to interact together to allow the binding of hiv to cd4+ cells. we have utilized enzymatic digestion by endoglycosidases in order to analyze n-linked carbohydrate chains of these proteins and their possible role in the interaction of gp120 or gp160 with cd4. sds denaturation was not necessary to obtain optimal deglycosylation of either molecule, but deglycosylation of cd4, nonetheless, depended on the presence of 1% triton x-100. endo h and ... | 1989 | 2538547 |
| characterization of the bacteriophage t4 gene 41 dna helicase. | the t4 gene 41 protein and the gene 61 protein function together as a primase-helicase within the seven protein bacteriophage t4 multienzyme complex that replicates duplex dna in vitro. we have previously shown that the 41 protein is a 5' to 3' helicase that requires a single-stranded region on the 5' side of the duplex to be unwound and is stimulated by the 61 protein (venkatesan, m., silver l. l., and nossal, n. g. (1982) j. biol. chem. 257, 12426-12434). the 41 protein, in turn, is required f ... | 1989 | 2538456 |
| bronchoalveolar lavage analysis, gallium-67 lung scanning and soluble interleukin-2 receptor levels in asbestos exposure. | this study examined different markers of lung immunologic and inflammatory responses to previous asbestos exposure. we performed bronchoalveolar lavage (bal) and gallium-67 (67ga) lung scans and measured serum and bal soluble interleukin-2 receptor (il-2r) and angiotensin-converting enzyme (sace) levels in 32 subjects with a history of significant asbestos exposure, 14 without (exp) and 18 with (asb) radiographic evidence of asbestosis. bal analysis revealed increases in neutrophils in both asb ... | 1989 | 2538325 |
| evidence that an increase in cytoplasmic calcium is the initiating event in certain plasma membrane-mediated responses to 3,5,3'-triiodothyronine in rat thymocytes. | the present studies were undertaken to explore further the mechanism by which t3 increases adenylate cyclase activity and the uptake of the sugar analog 2-deoxyglucose (2-dg) in freshly isolated rat thymocytes. in studies of cells preloaded with the fluorescent probe quin-2, whose fluorescence intensity increases linearly with increases in cytoplasmic free calcium concentration [( ca2+]i), we have now demonstrated that t3 increases [ca2+]i in thymocytes suspended in buffer containing 1 mm ca2+. ... | 1989 | 2538316 |
| listeria monocytogenes meningitis. | the history and findings of all patients with listeria meningitis admitted to the university hospital of leuven from 1967 to 1987 were reviewed. listeriosis during pregnancy or the perinatal natal period was not considered. predisposing conditions in these 23 patients included renal transplants (9), immunosuppressive therapy (2), diseases of the lympthoreticular system (3) and chronic alcoholism (1). one man had an inversed t4/t8 ratio. in 7 patients no underlying disorder was detected. disease ... | 1989 | 2538278 |
| bacteriophage t4 dna topoisomerase is the target of antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-amsa) in t4-infected escherichia coli. | the mammalian type ii dna topoisomerase has been proposed to be the intracellular target of a variety of antitumor agents, including m-amsa [4'-(9-acridinylamino)-methanesulfon-m-anisidide]. because the bacteriophage t4-encoded topoisomerase resembles the mammalian enzyme, we are using t4 as a simple model system to investigate the mechanism of action of m-amsa. a mutation that renders t4 growth m-amsa-resistant is closely linked to an amber mutation in t4 gene 39, which encodes one of the topoi ... | 1989 | 2537494 |
| paradoxically subnormal serum t4 and t3 in dogs after prolonged excessive tsh stimulation of the thyroid caused by post-camp refractoriness of thyroid hormone secretion. | several studies have demonstrated that excessive tsh stimulation of the thyroid for hours to days makes the thyroid gland less responsive to tsh. in this study we extended the period of excessive tsh stimulation of dog thyroids in vivo to 28 days. serum t4 and t3 were considerably elevated initially but then gradually declined and became paradoxically subnormal after 3 weeks. this was not caused by production of tsh antibodies. at day 28 the thyroid lobes were perfused for measurements of t4 and ... | 1989 | 2537453 |
| cardiac hypertrophy induced by thyroid hormone is independent of loading conditions and beta adrenoceptor blockade. | this study was designed to determine whether thyroid hormone (t4) produces cardiac hypertrophy and alters ventricular function by direct effects on the heart or by alterations in adrenergic stimulation or changes in the peripheral circulation. rats were treated with captopril (4 mg/ml of drinking water), propranolol (0.5 mg/ml of drinking water), hydralazine (80 mg/l of drinking water) or the combination of captopril and propranolol with and without t4 (15 micrograms/100 g b.w. i.p.). after 10 d ... | 1989 | 2537405 |
| a histopathological, immunohistochemical and ultrastructural study of intranuclear cytoplasmic inclusions in thyroid papillary carcinoma. | intranuclear cytoplasmic inclusions (ici) in 38 cases of thyroid papillary carcinoma were studied histopathologically, immunohistochemically, and ultrastructurally in order to examine the frequency of ici and their relationship to both the histological structure and cytological findings in thyroid papillary carcinoma. the fine-structure and biochemical state of ici were also studied. ici occurred in all 38 cases. ici occurrence ranged from one in several microscopic fields to more than ten per f ... | 1989 | 2536977 |
| endotoxin-induced reduction of beta-adrenergic binding sites on splenic lymphocytes in vivo and in vitro: its modulation by anterior hypothalamic lesions. | bacterial endotoxin induced a 38% decrease in the number of beta-adrenergic binding sites (bmax) on splenic lymphocytes, four days after intraperitoneal administration to guinea pigs. no change in the affinity (kd) for [125-i]-cyanopindolol ([125-i]-cyp) binding was observed. incubation of guinea pig splenocytes in vitro with different concentrations of bacterial endotoxin for 24 hours resulted in an increased incorporation of [3h]-thymidine, a parameter for lymphocyte activation. activation of ... | 1989 | 2536451 |
| inhibition of potentially lethal dna damage repair in human tumor cells by beta-lapachone, an activator of topoisomerase i. | a 4-h posttreatment with 4 microm beta-lapachone was previously shown to enhance the lethality of x-rays against human laryngeal epidermoid carcinoma (hep-2) cells (d. a. boothman et al., cancer res., 47:5361-5366, 1988). we now show that beta-lapachone (a) activates the dna-unwinding activity of topoisomerase i, (b) inhibits the fast component of potentially lethal damage repair (pldr) carried out by hep-2 cells when present during or immediately following x-irradiation, (c) specifically and sy ... | 1989 | 2535961 |
| cellular and humoral modifications during response to hbsag vaccine in healthy subjects: comparison between french and american vaccines. | two groups of 18 healthy adults, chosen from a risk population, were vaccinated against hepatitis b virus with french (hevac b, pasteur) or american (hb-vax, msd) vaccine; immunological and biochemical parameters (hbsab titers, ig, igm-rheumatoid factor (igm-rf) levels, autologous mixed lymphocyte reaction (amlr), t4/t8 ratio, lymphocyte migration inhibition test (lmit) with specific antigen and transaminase levels) were compared at preselected time intervals. the vaccines were found to be clini ... | 1989 | 2535246 |
| [influence of congenital hypothyroidism on the developing rat brain and improvement after thyroid replacement therapy]. | on the basis of rat model of congenital hypothyroidism, the following problems were investigated: (1) the biochemical changes of dna amount in cerebrum, and increase of dna concentration & decline of protein/dna in cerebellum. (2) the mitochondria atpase activity of the cells in cerebral cortex was significantly diminished in hypothyroidism, suggesting that energy metabolism of brain was affected by thyroid hormone. (3) the serum t4 level of hypothyroid offsprings was markedly decreased with inc ... | 1989 | 2534592 |
| [anomalies in thyroid function in children with trisomy 21]. | thyroid function of 60 children with down (ds) aged 3 months to 16 years was studied by evaluation of serum concentration of ultra-sensitive thyroid stimulating hormone (tsh), free t4 and t3 (ft4, ft3), total t4 and t3 (t4 and t3) and reverse t3 (rt3). each ds child was matched to a control of the same age. the concentration of tsh was increased in ds children while the concentration of rt3 of the ds children was significantly decreased compared to the controls as was the ratio rt3/tsh. these re ... | 1989 | 2534560 |
| studies on peripheral t lymphocyte subsets in patients with autoimmune thyroid diseases. | peripheral t lymphocyte subsets in 60 cases of graves' disease (gd) and 16 cases of hashimoto's thyroiditis (ht) were determined by the use of okt monoclonal antibodies (mcab). the results showed that in both diseases okt3 cells (total t cells) and okt8 cells (suppressor t cells, ts cells) were lower and the ratio of okt4 cells (helper t cells, th cells) okt8 cells was much higher than those in normal controls. gd patients who were treated with antithyroid drugs (atd) and whose serum t3, t4 reco ... | 1989 | 2534289 |
| abnormal t-lymphocyte subsets in type i diabetes. | type i (insulin-dependent) diabetes mellitus is a slow autoimmune disease associated with the selective destruction of beta-cells in the islets of langerhans. recent studies in humans indicate that autoantibodies to insulin and islets of langerhans appear years before overt diabetes and identify a normoglycemic prediabetic state. to determine whether type i diabetes mellitus represents a generalized immunologic disorder, we studied the phenotypic characteristics of peripheral blood lymphocytes f ... | 1989 | 2533573 |
| [studies on the pathogenesis of atherosclerosis]. | studies on the pathogenesis of atherosclerosis (as) were reviewed in our institute. clinico-pathological study showed that coronary as lesions appeared early and took a long-time to seriously stenosis the arterial lumen, resulting in coronary heart disease. studies on human as lesions by light and electron microscopy suggested that injury to and increased permeability of endothelial cells (ec) and proliferation of smooth muscle cells (smc), together with an increase of proteoglycans(pg) were imp ... | 1989 | 2532968 |
| [peripheral lymphocyte populations in patients treated with pelvic and mediastinal radiotherapy]. | solid tumor bearing patients often show a large variety of immunologic alterations: increase in immune complexes number, reduced nk activity, decreased il-2 production, t4/t8 reverse and s.o. most literature data generally concern chemotherapy and/or radiotherapy pretreated patients, so it is difficult to relate the immunological alterations with either antineoplastic treatments or the disease itself. we tried to evaluate any possible alteration of immunological parameters in patients with solid ... | 1989 | 2532757 |
| [peripheral thyroid insufficiency. criteria of equilibrium in treatment with l-thyroxine]. | during treatment of primary hypothyroidism, the respective values of hormonal assays (total and free t4 and t3, ultrasensitive tsh assay, trh test) and of the serum markers from peripheral tissue response to thyroid hormones used to detect overdosage are matter to debate. thirty-four female patients with hypothyroidism, aged 38 +/- 7 years (mean +/- sd) were studied. the mean dose of l-thyroxine required to obtain clinical euthyroidism was 100 +/- 35 micrograms/day. thyroid hormone and tsh conce ... | 1989 | 2531409 |
| [cancer of the gallbladder. a surgical experience]. | between 1965 and 1987, carcinoma of the gallbladder was found in 65 patients, 59 of whom were operated upon. seventy-seven per cent of these patients were women. mean age was 68 years. cholelithiasis was present in 77 per cent of the patients. since 1977, the preoperative diagnosis was made by ultrasonography in 43 per cent of the cases. the overall actuarial 1-year, 5-year and 10-year survival rates were 30, 13 and 13 per cent respectively. for patients whose tumour was limited to the gallbladd ... | 1989 | 2531402 |
| stereoselective arginine binding is a phylogenetically conserved property of group i self-splicing rnas. | we have examined the reaction of gtp with rna polymerase transcripts containing the self-splicing rna precursors from the neurospora crassa cob1 intron, and from introns in the suny, nrdb and td genes of bacteriophage t4. in each case, we find a low km for gtp (between 0.8 and 11 microm), accompanied by competitive inhibition of the gtp reaction by l-arginine, as was found for the previously examined tetrahymena nuclear pre-rrna intron. trials with the 20 standard amino acids show that inhibitio ... | 1989 | 2531082 |
| effects of a low-phosphorus, low-nitrogen diet supplemented with essential amino acids and ketoanalogues on serum beta-endorphin in chronic renal failure. | the effects of a vegetarian low-protein, low-phosphorus diet supplemented with essential amino acids and ketoanalogues, on the serum beta-endorphin, growth hormone, parathyroid hormone, thyroid hormones (t3 and t4), pituitary tsh and total cortisol were studied in 12 male chronic uremics. beta-endorphin decreased, as well as growth hormone. parathyroid hormone and t3 improved significantly, reaching almost normal values. it is hypothesized that the correction of the beta-endorphin excess may acc ... | 1989 | 2530457 |
| microglial cells in human brain have phenotypic characteristics related to possible function as dendritic antigen presenting cells. | the resting human microglia have previously been shown to be cells of dendritic morphology expressing class ii mhc antigens and macrophage specific antigens by immunocytochemical techniques. to examine the relationship between the microglia and the family of dendritic antigen presenting cells (apc), normal white matter from eight normal adults with no neurological disease at autopsy was examined by immunocytochemical techniques to localize antibodies to leukocyte common antigen (lca), hla-dr, cd ... | 1989 | 2530324 |
| cell count and ratio of helper/inducer to suppressor/cytotoxic t-cells in the cerebrospinal fluid of patients with multiple sclerosis. | the cell count and the helper/inducer to suppressor/cytotoxic t-cell ratio (t4:t8) in cerebrospinal fluid (csf) were compared in patients with multiple sclerosis (ms) and other neurological diseases. the t4:t8 ratio was higher in patients with ms than in patients with other neurological diseases. a low or normal csf cell count was associated with a more significant increase in the t4:t8 ratio in patients with ms. these findings imply that the increased t4:t8 ratio found in ms is masked by an inf ... | 1989 | 2530316 |
| common variable immunodeficiency: a family study and therapeutic trial with cimetidine. | various immunologic parameters were tested in a family of two patients with common variable immunodeficiency. both patients had low serum immunoglobulin levels, low peripheral b cell and t4 subclass of lymphocytes, and reversed t4/t8 ratio. one of the patients had excessive suppressor activity. two of the asymptomatic members of the family (the father and one brother) had also low t4/t8 ratio that was not associated with excessive suppressor activity. no linkage between the disease inheritance t ... | 1989 | 2530265 |