Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| progression of chronic periodontitis can be predicted by the levels of porphyromonas gingivalis and treponema denticola in subgingival plaque. | chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. diagnosis is achieved retrospectively by clinical observation of attachment loss. predicting disease progression would allow for targeted preventive therapy. the aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of ... | 2009 | 19832799 |
| cryo-electron tomography elucidates the molecular architecture of treponema pallidum, the syphilis spirochete. | cryo-electron tomography (cet) was used to examine the native cellular organization of treponema pallidum, the syphilis spirochete. t. pallidum cells appeared to form flat waves, did not contain an outer coat and, except for bulges over the basal bodies and widening in the vicinity of flagellar filaments, displayed a uniform periplasmic space. although the outer membrane (om) generally was smooth in contour, om extrusions and blebs frequently were observed, highlighting the structure's fluidity ... | 2009 | 19820083 |
| role of the cysteine protease interpain a of prevotella intermedia in breakdown and release of haem from haemoglobin. | the gram-negative oral anaerobe prevotella intermedia forms an iron(iii) protoporphyrin ix pigment from haemoglobin. the bacterium expresses a 90 kda cysteine protease, inpa (interpain a), a homologue of streptococcus pyogenes streptopain (speb). the role of inpa in haemoglobin breakdown and haem release was investigated. at ph 7.5, inpa mediated oxidation of oxyhaemoglobin to hydroxymethaemoglobin [in which the haem iron is oxidized to the fe(iii) state and which carries oh- as the sixth co-ord ... | 2009 | 19814715 |
| global challenge of antibiotic-resistant treponema pallidum. | syphilis is a multistage infectious disease that is usually transmitted through contact with active lesions of a sexual partner or from an infected pregnant woman to her fetus. despite elimination efforts, syphilis remains endemic in many developing countries and has reemerged in several developed countries, including china, where a widespread epidemic recently occurred. in the absence of a vaccine, syphilis control is largely dependent upon identification of infected individuals and treatment o ... | 2010 | 19805553 |
| global challenge of antibiotic-resistant treponema pallidum. | syphilis is a multistage infectious disease that is usually transmitted through contact with active lesions of a sexual partner or from an infected pregnant woman to her fetus. despite elimination efforts, syphilis remains endemic in many developing countries and has reemerged in several developed countries, including china, where a widespread epidemic recently occurred. in the absence of a vaccine, syphilis control is largely dependent upon identification of infected individuals and treatment o ... | 2010 | 19805553 |
| saliva enables the antimicrobial activity of ll-37 in the presence of proteases of porphyromonas gingivalis. | proteolysis is a common microbial virulence mechanism that enables the destruction of host tissue and evasion from host defense mechanisms. antimicrobial peptides, also known as host defense peptides, are effector molecules of the innate immunity that demonstrate a broad range of antimicrobial and immunoregulatory activities. deficiency of the human ll-37 antimicrobial peptide was previously correlated with severe periodontal disease. porphyromonas gingivalis, the major pathogen associated with ... | 2009 | 19805540 |
| salivary interleukin-1beta concentration and the presence of multiple pathogens in periodontitis. | this study aimed to find salivary enzymes and/or cytokines that would reflect periodontitis, alone or in combination with salivary microbial markers. | 2009 | 19799718 |
| characterization and structure of the manganese-responsive transcriptional regulator scar. | the streptococcal coaggregation regulator (scar) of streptococcus gordonii is a manganese-dependent transcriptional regulator. when intracellular manganese concentrations become elevated, scar represses transcription of the scacba operon, which encodes a manganese uptake transporter. a member of the dtxr/mntr family of metalloregulators, scar shares sequence similarity with other family members, and many metal-binding residues are conserved. here, we show that scar is an active dimer, with two d ... | 2009 | 19795834 |
| distribution of periodontopathic bacterial species in japanese children with developmental disabilities. | recent developments in molecular biological techniques have enabled rapid detection of periodontopathic bacterial species in clinical specimens. accumulated evidence suggests that detection of specific bacterial species enables identification of subjects at high risk for the onset of periodontitis. we investigated the distribution of 10 selected periodontopathic bacterial species in dental plaque specimens obtained from children with disabilities who were attending daycare centers. | 2009 | 19772671 |
| tnf-alpha and antibodies to periodontal bacteria discriminate between alzheimer's disease patients and normal subjects. | the associations of inflammation/immune responses with clinical presentations of alzheimer's disease (ad) remain unclear. we hypothesized that tnf-alpha and elevated antibodies to periodontal bacteria would be greater in ad compared to normal controls (nl) and their combination would aid clinical diagnosis of ad. plasma tnf-alpha and antibodies against periodontal bacteria were elevated in ad patients compared with nl and independently associated with ad. the number of positive igg to periodonta ... | 2009 | 19767111 |
| an unusual autopsy case of pyogenic liver abscess caused by periodontal bacteria. | pyogenic liver abscess (pla) formation is thought to originate from the transmission of infection via three major routes including the biliary tract, portal vein and hepatic artery. however, about 50% of pla cases are considered to be cryptogenic. here we report an unusual autopsy case of pla associated with periodontopathic bacterial infection. a 59-year-old female suddenly developed cardiopulmonary arrest and died. despite macroscopic and microscopic examinations, the infectious routes and sou ... | 2009 | 19762989 |
| elafin is specifically inactivated by rgpb from porphyromonas gingivalis by distinct proteolytic cleavage. | abstract porphyromonas gingivalis, the major causative bacterium of periodontitis, contributes significantly to elevated proteolytic activity at periodontal pockets owing to the presence of both bacteria and host, predominantly neutrophil-derived, serine proteases. normally the activity of the latter enzymes is tightly regulated by endogenous proteins, including elafin, a potent neutrophil elastase and proteinase 3 inhibitor released from epithelial cells at sites of inflammation. here, we repor ... | 2009 | 19747076 |
| pathophysiology of age-related diseases. | a symposium regarding the pathophysiology of successful and unsuccessful ageing was held in palermo, italy on 7-8 april 2009. three lectures from that symposium by g. campisi, l. ginaldi and f. licastro are here summarized. ageing is a complex process which negatively impacts on the development of various bodily systems and its ability to function. a long life in a healthy, vigorous, youthful body has always been one of humanity's greatest dreams. thus, a better understanding of the pathophysiol ... | 2009 | 19737378 |
| crystallization and preliminary x-ray analysis of betac-s lyases from two oral streptococci. | hydrogen sulfide, which causes oral malodour, is generally produced from l-cysteine by the action of betac-s lyase from oral bacteria. the betac-s lyases from two oral bacteria, streptococcus anginosus and s. gordonii, have been cloned, overproduced, purified and crystallized. x-ray diffraction data were collected from the two types of crystals using synchrotron radiation. the crystal of s. anginosus betac-s lyase belonged to the orthorhombic space group p2(1)2(1)2(1), with unit-cell parameters ... | 2009 | 19724121 |
| clinical and microbiological benefits of strict supragingival plaque control as part of the active phase of periodontal therapy. | to compare the clinical and microbiological effects of scaling and root planing (srp) alone or combined with mechanical [professional plaque control (ppc)] or chemical [chlorhexidine rinsing (chx)] control of supragingival plaque in the treatment of chronic periodontitis. | 2009 | 19703236 |
| oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in t cells, macrophages and dendritic cells. | the human immunodeficiency virus (hiv) can integrate into t cells, macrophages and dendritic cells resulting in a latent infection. reports have also demonstrated that various microbial and host cell factors can trigger hiv reactivation leading to hiv recrudescence, potentially undermining highly active antiretroviral therapies. | 2009 | 19702954 |
| quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time pcr. | the present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (pcr). twenty patients with untreated chronic periodontitis were randomized into two groups. before subgingival instrumentation and after 10 weeks samples in group a were taken first with a paper point half length (hp) of the probing depth, then with a paper point full length (fp) at the same site. in gro ... | 2010 | 19693550 |
| association between periodontitis and anti-cardiolipin antibodies in buerger disease. | anti-cardiolipin (cl) antibodies can be induced in buerger disease (bd), an inflammatory occlusive disorder affecting peripheral blood vessels, in response to bacteria bearing homology to the tlrvyk peptide of a phospholipid-binding plasma protein beta-2-glycoprotein i. tlrvyk homologies are present in porphyromonas gingivalis (tlriyt) and treponema denticola (tlalyk). this study investigated the association between periodontal infection and anti-cl antibodies in bd patients. | 2009 | 19678860 |
| efflux-mediated drug resistance in bacteria: an update. | drug efflux pumps play a key role in drug resistance and also serve other functions in bacteria. there has been a growing list of multidrug and drug-specific efflux pumps characterized from bacteria of human, animal, plant and environmental origins. these pumps are mostly encoded on the chromosome, although they can also be plasmid-encoded. a previous article in this journal provided a comprehensive review regarding efflux-mediated drug resistance in bacteria. in the past 5 years, significant pr ... | 2009 | 19678712 |
| global proteome analysis of leptospira interrogans. | comparative global proteome analyses were performed on leptospira interrogans serovar copenhageni grown under conventional in vitro conditions and those mimicking in vivo conditions (iron limitation and serum presence). proteomic analyses were conducted using itraq and lc-esi-tandem mass spectrometry complemented with two-dimensional gel electrophoresis and maldi-tof mass spectrometry. a total of 563 proteins were identified in this study. altered expression of 65 proteins, including upregulatio ... | 2009 | 19663501 |
| strong and persistent microbial and inflammatory stimuli overcome the genetic predisposition to higher matrix metalloproteinase-1 (mmp-1) expression: a mechanistic explanation for the lack of association of mmp1-1607 single-nucleotide polymorphism genotypes with mmp-1 expression in chronic periodontitis lesions. | our objective was to evaluate the association between the mmp1-1607 single-nucleotide polymorphism (snp), periodontopathogens and inflammatory cytokines with matrix metalloproteinase-1 (mmp-1) mrna levels in vitro and in vivo. | 2009 | 19659894 |
| microbiological risk for periodontitis of abutment teeth in patients with removable partial dentures. | tannerella forsythia, porphyromonas gingivalis and treponema denticola are identified as the red complex which exists in high proportions in periodontally diseased patients. the aim of this study was to assess the microbiological risk for periodontitis of abutment teeth in subjects wearing removable partial dentures (rpds) by measuring the red complex using bana-zymetm test. thirty-eight subjects (mean age: 62.2 years) with kennedy class ii partially edentulous arch participated in this study. t ... | 2009 | 19650858 |
| microbiological profile of untreated subjects with localized aggressive periodontitis. | the microbial profile of localized aggressive periodontitis (lagp) has not yet been determined. therefore, the aim of this study was to evaluate the subgingival microbial composition of lagp. | 2009 | 19637996 |
| characterizing traditionally defined periodontal disease in hiv+ adults. | results have varied from previous studies examining the level and extent of periodontal disease (pd) in hiv-1 infected (hiv+) adults. these studies used different methodologies to measure and define pd and examined cohorts with divergent characteristics. inconsistent methodological approaches may have resulted in the underestimation of traditionally-defined pd in hiv+ individuals. | 2009 | 19624697 |
| hydrogen sulfide induces apoptosis in human periodontium cells. | the existence of hydrogen sulfide (h(2)s) at high concentrations in periodontal pockets is a characteristic feature of periodontitis. periodontal pathogens play a key role in the production of h(2)s under these etiology conditions. this study was designed to examine the cytotoxicity of h(2)s in periodontium cells, including human periodontal ligament (pdl) cells and human gingival fibroblasts (hgfs), as well as the role of h(2)s in apoptosis induction. | 2010 | 19602114 |
| a novel murine model for chronic inflammatory alveolar bone loss. | chronic inflammatory bowel disease (ibd) demonstrates some similarities to the dysregulated chronic immunoinflammatory lesion of periodontitis. trinitrobenzene sulphonic acid (tnbs) and dextran sodium sulphate (dss) administered to rodents have been shown to elicit inflammatory responses that undermine the integrity of the gut epithelium in a similar manner to ibd in humans. the objective of this study was to evaluate the ability of these chemicals to elicit periodontal inflammation as a novel m ... | 2010 | 19602109 |
| a novel murine model for chronic inflammatory alveolar bone loss. | chronic inflammatory bowel disease (ibd) demonstrates some similarities to the dysregulated chronic immunoinflammatory lesion of periodontitis. trinitrobenzene sulphonic acid (tnbs) and dextran sodium sulphate (dss) administered to rodents have been shown to elicit inflammatory responses that undermine the integrity of the gut epithelium in a similar manner to ibd in humans. the objective of this study was to evaluate the ability of these chemicals to elicit periodontal inflammation as a novel m ... | 2010 | 19602109 |
| involvement of toll-like receptors 2 and 4 in the innate immune response to treponema denticola and its outer sheath components. | treponema denticola is considered an important oral pathogen in the development and progression of periodontal diseases. in the present study, the mechanisms of recognition and activation of murine macrophages by t. denticola and its major outer sheath protein (msp) and lipooligosaccharide (los or glycolipid) were investigated. t. denticola cells and the msp induced innate immune responses through tlr2-myd88, whereas los induced a macrophage response through tlr4-myd88. the presence of gamma int ... | 2009 | 19596768 |
| is obesity an oral bacterial disease? | the world-wide explosion of overweight people has been called an epidemic. the inflammatory nature of obesity is widely recognized. could it really be an epidemic involving an infectious agent? in this climate of concern over the increasing prevalence of overweight conditions in our society, we focus on the possible role of oral bacteria as a potential direct contributor to obesity. to investigate this possibility, we measured salivary bacterial populations of overweight women. saliva was collec ... | 2009 | 19587155 |
| detection and quantification of five major periodontal pathogens by single copy gene-based real-time pcr. | periodontitis is a common chronic multibacterial infection in the tooth-supporting tissues. it has been shown that periodontitis patients carry higher number of disease-associated bacteria than healthy ones. the aim of this study was to generate a novel, single copy gene-based quantitative real-time pcr (qpcr) assay for five major periodontal pathogens - aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, treponema denticola, and tannerella forsythia. the prim ... | 2009 | 19586997 |
| egfrviii and c-met pathway inhibitors synergize against pten-null/egfrviii+ glioblastoma xenografts. | receptor tyrosine kinase (rtk) systems, such as hepatocyte growth factor (hgf) and its receptor c-met, and epidermal growth factor receptor (egfr), are responsible for the malignant progression of multiple solid tumors. recent research shows that these rtk systems comodulate overlapping and dynamically adaptable oncogenic downstream signaling pathways. this study investigates how egfrviii, a constitutively active egfr deletion mutant, alters tumor growth and signaling responses to rtk inhibition ... | 2009 | 19584231 |
| plasminogen acquisition and activation at the surface of leptospira species lead to fibronectin degradation. | pathogenic leptospira species are the etiological agents of leptospirosis, a widespread disease of human and veterinary concern. in this study, we report that leptospira species are capable of binding plasminogen (plg) in vitro. the binding to the leptospiral surface was demonstrated by indirect immunofluorescence confocal microscopy with living bacteria. the plg binding to the bacteria seems to occur via lysine residues because the ligation is inhibited by addition of the lysine analog 6-aminoc ... | 2009 | 19581392 |
| proteomic analysis of endodontic infections by liquid chromatography-tandem mass spectrometry. | endodontic infections are very prevalent and have a polymicrobial etiology characterized by complex interrelationships between endodontic microorganisms and the host defenses. proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the host in the initiation and progression of apical periodontitis. the purpose of this study was to apply proteomic techniques such a ... | 2009 | 19572900 |
| the influence of tobacco smoking on the onset of periodontitis in young persons. | abstract : this paper reviews the evidence for cigarette smoking as a risk factor for the development of severe destructive periodontal disease in young adults. a high prevalence of cigarette smoking has been identified among young individuals with aggressive periodontitis and tobacco usage increases the risk of periodontal destruction most significantly in young populations. the effect appears to be dose related and is independent of levels of plaque accumulation. young smokers have more alveol ... | 2004 | 19570272 |
| comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque samples. | to compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples. | 2009 | 19563330 |
| periodontitis in pregnancy: clinical and serum antibody observations from a baboon model of ligature-induced disease. | chronic oral infections that elicit host responses leading to periodontal disease are linked with various sequelae of systemic diseases. this report provides seminal information on the clinical and adaptive immunologic characteristics of a baboon model of ligature-induced periodontitis during pregnancy. | 2009 | 19563297 |
| clinical effectiveness of photodynamic therapy in the treatment of periodontitis. | a randomized-controlled clinical pilot trial was designed to evaluate photodynamic therapy (pdt) for its bactericidal potential and clinical effect in the treatment of periodontitis. | 2009 | 19554711 |
| major proteins and antigens of treponema denticola. | treponema denticola is a gram-negative, motile, asaccharolytic, anaerobic spirochaete which along with porphyromonas gingivalis and tannerella forsythia has been shown to form a bacterial consortium called the red complex that is strongly associated with the clinical progression of chronic periodontitis. t. denticola was grown in continuous culture in a complex medium with a mean generation time of 15.75 h. samples from two different membrane-enriched preparations and a cytoplasm-enriched prepar ... | 2009 | 19501677 |
| association of systemic oxidative stress with suppressed serum igg to commensal oral biofilm and modulation by periodontal infection. | to assess the impact of systemic oxidative stress on humoral immune responses, we examined the relation between levels of serum 8-isoprostane and serum igg antibodies against 17 microorganisms in the commensal oral biofilm among the aric population of community-dwelling adults (n = 4,717). bivariately, serum 8-isoprostane was associated with age, race/center, education, smoking, serum triglycerides, and the extent of periodontal disease severity. total igg antibody directed to the oral biofilm w ... | 2009 | 19496702 |
| systemic immune responses in pregnancy and periodontitis: relationship to pregnancy outcomes in the obstetrics and periodontal therapy (opt) study. | our previous studies reported on the obstetric, periodontal, and microbiologic outcomes of women participating in the obstetrics and periodontal therapy (opt) study. this article describes the systemic antibody responses to selected periodontal bacteria in the same patients. | 2009 | 19485826 |
| the oral microbiota: living with a permanent guest. | the oral cavity of healthy individuals contains hundreds of different bacterial, viral, and fungal species. many of these can associate to form biofilms, which are resistant to mechanical stress or antibiotic treatment. most are also commensal species, but they can become pathogenic in responses to changes in the environment or other triggers in the oral cavity, including the quality of an individual's personal hygiene. the complexity of the oral microbiome is being characterized through the new ... | 2009 | 19485767 |
| distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by porphyromonas gingivalis. | porphyromonas gingivalis, a periodontal pathogen, expresses a number of virulence factors, including long (fima) and short (mfa) fimbriae as well as gingipains comprised of arginine-specific (rgp) and lysine-specific (kgp) cysteine proteinases. the aim of this study was to examine the roles of these components in homotypic biofilm development by p. gingivalis, as well as in accumulation of exopolysaccharide in biofilms. | 2009 | 19470157 |
| microbial changes in periodontitis successfully treated by mechanical plaque removal and systemic amoxicillin and metronidazole. | scaling and root planing in conjunction with systemic administration of antibiotics is used for treatment of aggressive periodontitis. the study investigated the changes of the subgingival microbiota in a homogeneous cohort of 12 female caucasian patients. plaque samples were obtained from 4 defined deep lesions per patient at baseline and 2, 6, and 12 months after therapy (mechanical plaque removal, oral administration of amoxicillin and metronidazole). amplification of the 16s rrna gene, cloni ... | 2009 | 19447073 |
| the oral microbial consortium's interaction with the periodontal innate defense system. | the oral microbial consortium is the most characterized polymicrobial microbial community associated with the human host. extensive sampling of both microbial and tissue samples has demonstrated that there is a strong association between the type of microbial community found in the gingival crevice and the status of innate host mediator expression. the strong clinical association between the microbial community and the innate host response in both clinically healthy and diseased tissue suggests ... | 2009 | 19435427 |
| tp0262 is a modulator of promoter activity of tpr subfamily ii genes of treponema pallidum ssp. pallidum. | transcriptional regulation in treponema pallidum ssp. pallidum is poorly understood, primarily because this organism cannot be cultivated in vitro or genetically manipulated. we have recently shown a phase variation mechanism controlling transcription initiation of subfamily ii tpr (t. pallidumrepeat) genes (tpre, tprg and tprj), a group of virulence factor candidates. furthermore, the same study suggested that additional mechanisms might influence the level of transcription of these tprs. the t ... | 2009 | 19432808 |
| treponema pallidum, the stealth pathogen, changes, but how? | treponema pallidum rapidly disseminates from a genital site of inoculation to diverse organs where it establishes persistent infection. t. pallidum has long been regarded as a stealth pathogen because of its poorly antigenic and non-inflammatory surface. there is now increasing evidence that antigenic variation also contributes to the ability of the spirochaete to evade host defences. among the small number of proteins encoded by the t. pallidum genome with sequence similarity to well-characteri ... | 2009 | 19432802 |
| the use of perioscan as a potential screening test for mothers at risk for delivery of pre-term and low birth-weight infants. | increased levels of oestrogen and progesterone during pregnancy may lead to periodontal disease. the anaerobic gram-negative bacteria classified as the "red complex" (porphyromonas gingivalis, tannerella forsythia and treponema denticola) are frequently associated with periodontal disease. periodontopathogens produce toxins and enzymes that can enter the bloodstream and cross the placenta to harm the foetus. the response of the mother's immune system to infection by these periodontopathogens bri ... | 2009 | 19431959 |
| [markers of periodontal diseases and sensitivity to taromentine in patients with aggressive periodontitis]. | the aim of the research was to study sensitivity of specific microorganisms from the periodontal pockets of patients with rapidly progressive periodontal disease to taromentine. 95 patients aged 21 to 35 years (50 women (52,6+/-33,62) and 45 men (47,36+/-3,62)) with rapidly progressive form of periodontal desease were observed. porphiromonas gingivalis was identifide in 83 out of 95 patients (87,36+/-2,06). prevotella intermedia - in 31 patients (32,6+/-2,750); actinobacillus actinomycetemcomita ... | 2009 | 19430039 |
| intact flagellar motor of borrelia burgdorferi revealed by cryo-electron tomography: evidence for stator ring curvature and rotor/c-ring assembly flexion. | the bacterial flagellar motor is a remarkable nanomachine that provides motility through flagellar rotation. prior structural studies have revealed the stunning complexity of the purified rotor and c-ring assemblies from flagellar motors. in this study, we used high-throughput cryo-electron tomography and image analysis of intact borrelia burgdorferi to produce a three-dimensional (3-d) model of the in situ flagellar motor without imposing rotational symmetry. structural details of b. burgdorfer ... | 2009 | 19429612 |
| electron cryotomography: a new view into microbial ultrastructure. | electron cryotomography (ect) is an emerging technology that allows thin samples such as small bacterial cells to be imaged in 3d in a nearly native state to 'molecular' (approximately 4nm) resolution. as such, ect is beginning to deliver long-awaited insight into the positions and structures of cytoskeletal filaments, cell wall elements, motility machines, chemoreceptor arrays, internal compartments, and other ultrastructures. here we briefly explain ect, review its recent contributions to micr ... | 2009 | 19427259 |
| bacteria in the apical root canal of teeth with primary apical periodontitis. | bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis. | 2009 | 19426923 |
| heterogeneity of systemic inflammatory responses to periodontal therapy. | we investigated the effect of comprehensive periodontal therapy on the levels of multiple systemic inflammatory biomarkers. | 2009 | 19426174 |
| detection of treponema denticola in symptomatic apical periodontitis and in symptomatic apical abscesses by real-time pcr. | the aim of this study was to investigate the presence of treponema denticola in symptomatic apical periodontitis and in symptomatic apical abscesses by real-time polymerase chain reaction (pcr) method. | 2009 | 19421390 |
| role of aggregatibacter actinomycetemcomitans in glutathione catabolism. | our previous studies demonstrated that three enzymes, gamma-glutamyltransferase (ggt), cysteinylglycinase (cgase) and cystalysin, are required for the catabolism of glutathione to produce hydrogen sulfide (h(2)s) in treponema denticola. in this study, we examined glutathione catabolism in aggregatibacter actinomycetemcomitans. | 2009 | 19416454 |
| the microbiota on different oral surfaces in healthy children. | knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes. | 2009 | 19416446 |
| pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum 'synergistetes' isolated from the human oral cavity. | four strains of anaerobic, gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group distinct from any species with validly published names. 16s rrna and 23s rrna gene sequence analyses and dna-dna reassociation data revealed that the strains constituted a novel group within the phylum 'synergistetes' and were most closely related to jonquetella anthropi. two libraries of rand ... | 2009 | 19406777 |
| borrelia burgdorferi reva antigen binds host fibronectin. | borrelia burgdorferi, the lyme disease-causing spirochete, can persistently infect its vertebrate hosts for years. b. burgdorferi is often found associated with host connective tissue, where it interacts with components of the extracellular matrix, including fibronectin. some years ago, a borrelial surface protein, named bbk32, was identified as a fibronectin-binding protein. however, b. burgdorferi bbk32 mutants are still able to bind fibronectin, indicating that the spirochete possesses additi ... | 2009 | 19398540 |
| the oxyr homologue in tannerella forsythia regulates expression of oxidative stress responses and biofilm formation. | tannerella forsythia is an anaerobic periodontal pathogen that encounters constant oxidative stress in the human oral cavity due to exposure to air and reactive oxidative species from coexisting dental plaque bacteria as well as leukocytes. in this study, we sought to characterize a t. forsythia orf with close similarity to bacterial oxidative stress response sensor protein oxyr. to analyse the role of this oxyr homologue, a gene deletion mutant was constructed and characterized. aerotolerance, ... | 2009 | 19389765 |
| are putative periodontal pathogens reliable diagnostic markers? | periodontitis is one of the most common chronic inflammatory diseases. a number of putative bacterial pathogens have been associated with the disease and are used as diagnostic markers. in the present study, we compared the prevalence of oral bacterial species in the subgingival biofilm of generalized aggressive periodontitis (gap) (n = 44) and chronic periodontitis (cp) (n = 46) patients with that of a periodontitis-resistant control group (pr) (n = 21). the control group consisted of subjects ... | 2009 | 19386852 |
| coaggregation by the freshwater bacterium sphingomonas natatoria alters dual-species biofilm formation. | coaggregation is hypothesized to enhance freshwater biofilm development. to investigate this hypothesis, the ability of the coaggregating bacterium sphingomonas natatoria to form single- and dual-species biofilms was studied and compared to that of a naturally occurring spontaneous coaggregation-deficient variant. attachment assays using metabolically inactive cells were performed using epifluorescence and confocal laser scanning microscopy. under static and flowing conditions, coaggregating s. ... | 2009 | 19376917 |
| diversity and site-specificity of the oral microflora in the elderly. | the purpose of the present study was to describe the bacterial diversity in the oral cavity of the elderly without root caries using bacterial microarrays, and to determine the site- and subject-specificity of bacterial colonization. samples were collected from the tongue dorsum, mucosa of the buccal fold, hard palate, supragingival plaque from sound root surfaces, and subgingival plaque from the same roots. a new 16 s rrna gene-based microarray method was used for the simultaneous detection of ... | 2009 | 19373498 |
| red bacterial complex is associated with the severity of chronic periodontitis in a thai population. | the distribution of periodontal pathogens differs in various geographic locations and racial/ethnic groups. this study investigated the microbiological features of chronic periodontitis (cp) patients in thailand. | 2009 | 19371397 |
| oral infectious diseases: a potential risk factor for hiv virus recrudescence? | as the highly active antiretroviral therapy (haart) has transitioned human immunodeficiency virus (hiv) infection into a 'chronic disease' management strategy, there is growing evidence that infection with non-hiv pathogens in hiv+ patients may have important public health implications in undermining haart success and acquired immunodeficiency syndrome progression. several bacterial and host cell products during infections with non-hiv pathogens have shown the capacity to regulate hiv replicatio ... | 2009 | 19364391 |
| inhibition of selenium metabolism in the oral pathogen treponema denticola. | in this report we provide evidence that the antimicrobial action of stannous salts and a gold drug, auranofin, against treponema denticola is mediated through inhibition of the metabolism of selenium for synthesis of selenoproteins. | 2009 | 19363113 |
| porphyromonas gingivalis-host interactions: open war or intelligent guerilla tactics? | this review summarizes and discusses virulence mechanisms whereby porphyromonas gingivalis can persist in the oral cavity. it is proposed that the virulence of p. gingivalis is dependent, at least in part, upon its ability to establish a complex host-pathogen molecular crosstalk which subverts innate immunity. the sophisticated stealth and sabotage tactics used by p. gingivalis may additionally benefit co-habiting organisms occupying the same niche. | 2009 | 19348960 |
| diversity and morphology of members of the phylum "synergistetes" in periodontal health and disease. | members of the phylum "synergistetes" have frequently been detected in the human oral cavity at sites of dental disease, but they have rarely been detected in studies of oral health. only two oral "synergistetes" taxa are cultivable. the aims of this study were to investigate the diversity of "synergistetes" in the oral cavity, to establish whether "synergistetes" taxa are more strongly associated with periodontitis than with oral health, and to visualize unculturable "synergistetes" in situ. si ... | 2009 | 19346352 |
| a review of dental implants and infection. | dental implants have become increasingly common for the management of tooth loss. despite their placement in a contaminated surgical field, success rates are relatively high. this article reviews dental implants and highlights factors leading to infection and potential implant failure. a literature search identified studies analysing the microbial composition of peri-implant infections. the microflora of dental peri-implantitis resembles that found in chronic periodontitis, featuring predominant ... | 2009 | 19329223 |
| fusobacterium nucleatum atcc 10953 requires actinomyces naeslundii atcc 43146 for growth on saliva in a three-species community that includes streptococcus oralis 34. | formation of dental plaque is a developmental process involving initial and late colonizing species that form polymicrobial communities. fusobacteria are the most numerous gram-negative bacteria in dental plaque, but they become prevalent after the initial commensal colonizers, such as streptococci and actinomyces, have established communities. the unusual ability of these bacteria to coaggregate with commensals, as well as pathogenic late colonizers, has been proposed to facilitate colonization ... | 2009 | 19286780 |
| uncovering metabolic pathways relevant to phenotypic traits of microbial genomes. | identifying the biochemical basis of microbial phenotypes is a main objective of comparative genomics. here we present a novel method using multivariate machine learning techniques for comparing automatically derived metabolic reconstructions of sequenced genomes on a large scale. applying our method to 266 genomes directly led to testable hypotheses such as the link between the potential of microorganisms to cause periodontal disease and their ability to degrade histidine, a link also supported ... | 2009 | 19284550 |
| rapid and accurate diagnosis of human intestinal spirochetosis by fluorescence in situ hybridization. | human intestinal spirochetosis (his) is associated with overgrowth of the large intestine by spirochetes of the genus brachyspira. the microbiological diagnosis of his is hampered by the fastidious nature and slow growth of brachyspira spp. in clinical practice, his is diagnosed histopathologically, and a significant portion of cases may be missed. fluorescence in situ hybridization (fish) is a molecular method that allows the visualization and identification of single bacteria within tissue sec ... | 2009 | 19279178 |
| genome sequence of the pathogenic intestinal spirochete brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine. | brachyspira hyodysenteriae is an anaerobic intestinal spirochete that colonizes the large intestine of pigs and causes swine dysentery, a disease of significant economic importance. the genome sequence of b. hyodysenteriae strain wa1 was determined, making it the first representative of the genus brachyspira to be sequenced, and the seventeenth spirochete genome to be reported. the genome consisted of a circular 3,000,694 base pair (bp) chromosome, and a 35,940 bp circular plasmid that has not p ... | 2009 | 19262690 |
| diversity of spirochetes in endodontic infections. | the diversity of spirochetes in primary endodontic infections of teeth with chronic apical periodontitis or acute apical abscesses was investigated using 16s rrna gene clone library analysis. the prevalences of three common cultivable oral treponema species were also determined using species-specific nested pcr. all detected spirochetes belonged to the genus treponema. overall, 28 different taxa were identified from the 431 clones sequenced: 9 cultivable and validly named species, 1 cultivable a ... | 2009 | 19261792 |
| identification of pathogen and host-response markers correlated with periodontal disease. | periodontitis is the major cause of tooth loss in adults and is linked to systemic illnesses, such as cardiovascular disease and stroke. the development of rapid point-of-care (poc) chairside diagnostics has the potential for the early detection of periodontal infection and progression to identify incipient disease and reduce health care costs. however, validation of effective diagnostics requires the identification and verification of biomarkers correlated with disease progression. this clinica ... | 2009 | 19254128 |
| interpain a, a cysteine proteinase from prevotella intermedia, inhibits complement by degrading complement factor c3. | periodontitis is an inflammatory disease of the supporting structures of the teeth caused by, among other pathogens, prevotella intermedia. many strains of p. intermedia are resistant to killing by the human complement system, which is present at up to 70% of serum concentration in gingival crevicular fluid. incubation of human serum with recombinant cysteine protease of p. intermedia (interpain a) resulted in a drastic decrease in bactericidal activity of the serum. furthermore, a clinical stra ... | 2009 | 19247445 |
| effects of oral commensal and pathogenic bacteria on human dendritic cells. | the oral cavity harbors a diverse and complex microbial community. bacteria accumulate on both the hard and soft oral tissues in sessile biofilms and engage the host in an intricate cellular dialog, which normally constrains the bacteria to a state of commensal harmony. dendritic cells (dcs) are likely to balance tolerance and active immunity to commensal microorganisms as part of chronic inflammatory responses. while the role played by dcs in maintaining intestinal homeostasis has been investig ... | 2009 | 19239635 |
| bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from japanese subjects. | recent developments in molecular biological techniques have increased understanding of the distribution of oral bacterial species in clinical specimens, though few investigations have been conducted to simultaneously detect oral streptococcal and periodontal species in the same specimens. the purpose of the present study was to investigate the distribution and correlation of 6 oral streptococcal and 6 periodontal species in saliva specimens taken from children and their mothers. | 2009 | 19230860 |
| does pregnancy have an impact on the subgingival microbiota? | we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care. | 2009 | 19228092 |
| [detection of 3 anaerobic microorganisms in saliva and subgingival plaque of patients with periodontitis]. | to investigate the prevalence of 3 anaerobic microorganisms including porphyromonas gingivalis (pg), tannerella forsythia (tf) and treponema denticola (td) in saliva and pooled subgingival plaque of different types of periodontitis and compare the detections of three microorganisms between saliva and pooled subgingival plaque sample from the same patient, and analyze the relationship between prevalence of these pathogens in saliva and periodontal parameters. | 2009 | 19221563 |
| target selection and annotation for the structural genomics of the amidohydrolase and enolase superfamilies. | to study the substrate specificity of enzymes, we use the amidohydrolase and enolase superfamilies as model systems; members of these superfamilies share a common tim barrel fold and catalyze a wide range of chemical reactions. here, we describe a collaboration between the enzyme specificity consortium (enspec) and the new york sgx research center for structural genomics (nysgxrc) that aims to maximize the structural coverage of the amidohydrolase and enolase superfamilies. using sequence- and s ... | 2009 | 19219566 |
| hand instrumentation versus ultrasonic debridement in the treatment of chronic periodontitis: a randomized clinical and microbiological trial. | to compare the effectiveness of scaling and root planing (srp) with the use of hand instruments to that of non-surgical treatment with the use of an ultrasonic device, using clinical and microbiological criteria. | 2009 | 19207889 |
| minimum contradiction matrices in whole genome phylogenies. | minimum contradiction matrices are a useful complement to distance-based phylogenies. a minimum contradiction matrix represents phylogenetic information under the form of an ordered distance matrix y(i) (,) (j) (n). a matrix element corresponds to the distance from a reference vertex n to the path (i, j). for an x-tree or a split network, the minimum contradiction matrix is a robinson matrix. it therefore fulfills all the inequalities defining perfect order: y(i) (,) (j) (n) >or= y(i) (,) (k) (n ... | 2008 | 19204821 |
| identification of spirochetes associated with contagious ovine digital dermatitis. | spirochetes of the genus treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. two cultures comprised treponema phagedenis-like and treponema medium/treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of treponema medium/treponema vincentii-like, treponema phagedenis-like, and treponema denticola/treponema putidum-like organisms. | 2009 | 19204100 |
| analysis of a unique interaction between the complement regulatory protein factor h and the periodontal pathogen treponema denticola. | treponema denticola, a spirochete associated with periodontitis, is abundant at the leading edge of subgingival plaque, where it interacts with gingival epithelia. t. denticola produces a number of virulence factors, including dentilisin, a protease which is cytopathic to host cells, and fhbb, a unique t. denticola lipoprotein that binds complement regulatory proteins. earlier analyses suggested that fhbb specifically bound to factor h (fh)-like protein 1 (fhl-1). however, by using dentilisin-de ... | 2009 | 19204088 |
| the effect of diode laser (980 nm) treatment on aggressive periodontitis: evaluation of microbial and clinical parameters. | the aim was to compare the effect of scaling and root planing (srp) alone, diode laser treatment (las) alone, and srp combined with las (srp + las) on clinical and microbial parameters in patients with aggressive periodontitis. | 2009 | 19196111 |
| rickettsia phylogenomics: unwinding the intricacies of obligate intracellular life. | completed genome sequences are rapidly increasing for rickettsia, obligate intracellular alpha-proteobacteria responsible for various human diseases, including epidemic typhus and rocky mountain spotted fever. in light of phylogeny, the establishment of orthologous groups (ogs) of open reading frames (orfs) will distinguish the core rickettsial genes and other group specific genes (class 1 ogs or c1ogs) from those distributed indiscriminately throughout the rickettsial tree (class 2 og or c2ogs) ... | 2008 | 19194535 |
| design and activity of a 'dual-targeted' antimicrobial peptide. | numerous reports have indicated the important role of human normal flora in the prevention of microbial pathogenesis and disease. evidence suggests that infections at mucosal surfaces result from the outgrowth of subpopulations or clusters within a microbial community and are not linked to one pathogenic organism alone. to preserve the protective normal flora whilst treating the majority of infective bacteria in the community, a tuneable therapeutic is necessary that can discriminate between ben ... | 2009 | 19188046 |
| probing the macromolecular organization of cells by electron tomography. | a major goal in cell biology is to understand the functional organization of macromolecular complexes in vivo. electron microscopy is helping cell biologists to achieve this goal, thanks to its ability to resolve structural details in the nanometer range. while issues related to specimen preparation, imaging, and image interpretation make this approach to cell architecture difficult, recent improvements in methods, equipment, and software have facilitated the study of both important macromolecul ... | 2009 | 19185480 |
| tannerella forsythia and the hla-dqb1 allele are associated with susceptibility to periodontal disease in japanese adolescents. | periodontal disease is a multiple factor disease caused by genetic factors, environmental factors, and periodontal bacteria (periodontal pathogens). the present study aimed to elucidate the risk factors for periodontal disease in japanese adolescents. subjects (11-16 years old) were classified into three groups: localized aggressive periodontitis (lap), periodontal attachment loss (pal), and periodontally healthy (ph) groups. genomic dna isolated from the buccal mucosa was used for single-nucleo ... | 2009 | 19184295 |
| inflammation as a potential mediator for the association between periodontal disease and alzheimer's disease. | periodontal disease (pdd) is associated with increased risk of cardiovascular disease, cerebrovascular disease, and mortality in many studies, while other studies have begun to suggest an association of pdd with alzheimer's disease (ad). this paper discusses how infectious pathogens and systemic infection may play a role in ad. the roles of infection and inflammation are addressed specifically with regard to known ad pathologic lesions including senile plaques, neuron death, neurofibrillary tang ... | 2008 | 19183779 |
| tobacco-induced alterations to porphyromonas gingivalis-host interactions. | smokers are more susceptible than non-smokers to persistent infection by porphyromonas gingivalis, a causative agent of periodontitis. patients who smoke exhibit increased susceptibility to periodontitis and are more likely to display severe disease and be refractory to treatment. paradoxically, smokers demonstrate reduced clinical inflammation. we show that p. gingivalis cells exposed to cigarette smoke extract (cse) induce a lower proinflammatory response (tumour necrosis factor-alpha, interle ... | 2009 | 19175666 |
| auranofin disrupts selenium metabolism in clostridium difficile by forming a stable au-se adduct. | clostridium difficile is a nosocomial pathogen whose incidence and importance are on the rise. previous work in our laboratory characterized the central role of selenoenzyme-dependent stickland reactions in c. difficile metabolism. in this work we have identified, using mass spectrometry, a stable complex formed upon reaction of auranofin (a gold-containing drug) with selenide in vitro. x-ray absorption spectroscopy supports the structure that we proposed on the basis of mass-spectrometric data. ... | 2009 | 19165513 |
| salivary detection of periodontopathic bacteria and periodontal health status in dental students. | saliva may become a potential source of contamination through vertical and horizontal transmissions as well as cross-infections. this study aims to use saliva as a screening tool to detect putative periodontal pathogens in a young population with fairly good oral hygiene. | 2009 | 19162209 |
| porphyromonas gingivalis with either tannerella forsythia or treponema denticola induces synergistic il-6 production by murine macrophage-like j774.1 cells. | chronic periodontitis is caused by mixed bacterial infection. porphyromonas gingivalis, tannerella forsythia and treponema denticola are frequently detected in deep periodontal pockets. we demonstrate that these bacteria induce proinflammatory cytokine production by the mouse macrophage-like cell line j774.1. | 2009 | 19162208 |
| the vaginal microflora in relation to gingivitis. | gingivitis has been linked to adverse pregnancy outcome (apo). bacterial vaginosis (bv) has been associated with apo. we assessed if bacterial counts in bv is associated with gingivitis suggesting a systemic infectious susceptibilty. | 2009 | 19161595 |
| vaccines and photodynamic therapies for oral microbial-related diseases. | the mouth is a favorable habitat for a great variety of bacteria. microbial composition of dental plaque is the usual cause of various oral diseases in humans, including dental caries, periodontal disease and halitosis. in general, oral antibacterial agents such as antibiotics are commonly used to treat oral bacterial infection. traditional periodontal surgery is painful and time-consuming. in addition, bacterial resistance and toxicity of antibiotics have become a global pandemic and unavoidabl ... | 2009 | 19149517 |
| association of unique, isolated treponemes with bovine digital dermatitis lesions. | this study used a pcr-based approach targeting 16s rrna gene fragments to determine the occurrence and association of the three bovine digital dermatitis (bdd) treponeme phylogroups within lesions found in cattle from the united kingdom. examination of 51 bdd lesions collected from infected cattle across the united kingdom revealed that bdd treponeme group 1 (treponema medium/treponema vincentii-like), group 2 (treponema phagedenis-like), and group 3 (treponema putidum/treponema denticola-like) ... | 2009 | 19144804 |
| microbiology of odontogenic bacteremia: beyond endocarditis. | summary: the human gingival niche is a unique microbial habitat. in this habitat, biofilm organisms exist in harmony, attached to either enamel or cemental surfaces of the tooth as well as to the crevicular epithelium, subjacent to a rich vascular plexus underneath. due to this extraordinary anatomical juxtaposition, plaque biofilm bacteria have a ready portal of ingress into the systemic circulation in both health and disease. yet the frequency, magnitude, and etiology of bacteremias due to ora ... | 2009 | 19136433 |
| analysis of a band 7/mec-2 family gene of porphyromonas gingivalis. | in vivo-induced antigen technology has previously been used to identify 115 genes induced in porphyromonas gingivalis w83 during human infection. the aim of this study was to determine if one of these genes, pg1334, was important for the virulence of p. gingivalis. analysis of plaque samples from persons with periodontitis revealed that pg1334 was expressed in 88.0% of diseased sites, compared with 42.1% of healthy sites, even though p. gingivalis was detected in equal numbers from both sites. a ... | 2009 | 19131314 |
| short-term clinical and microbiological evaluations of peri-implant diseases before and after mechanical anti-infective therapies. | the aim of this study was to evaluate the clinical and microbiological effects of mechanical anti-infective therapies for mucositis and peri-implantitis. | 2009 | 19126114 |
| periodontal pathogen levels in adolescents before, during, and after fixed orthodontic appliance therapy. | this purpose of this study was to document and investigate changes in periodontal pathogen levels before, during, and after orthodontic treatment in adolescents. | 2009 | 19121507 |