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studies on sterol-ester hydrolase from fusarium oxysporum. i. partial purification and properties.1. a search for a long chain fatty acyl sterol-ester hydrolase in microorganisms led to the isolation from soil of five strains belonging to fusarium sp. which produced strong activity in the culture medium. 2. the cholesterol esterase from fusarium oxysporum igh-2 was purified about 270-fold by means of cacl2 precipitation and sephadex g-75 column chromatography. 3. the cholesterol esterase was activated by adekatol and triton x-100. it was inhibited by lecithin and lysolecithin, and completely ...197719426
mycetoma in somalia - results of a survey done from 1959 to 1964.the pasteur institute studied 103 mycetoma patients in somalia between 1959 and 1964. grains were seen in 94 of them and this, added to cultural features, allowed the diagnosis of 60 pathogens as follows: 44 madurella mycetomi, 1 leptosphaeria senegalensis, 7 pyrenochaeta romeroi (or madurella grisea), 3 allescheria boydii, 1 fusarium sp., 3 neotestudina (zopfia) rosatii, and 1 unidentified; 34 were actinomycetes: 24 streptomyces somaliensis, 4 actinomadura madurae, 3 a. pelletieri and 3 nocardi ...197723657
synthesis of cellulase by fusarium sp. in different culture conditions.the mechanism of the synthesis of cellulases by fusarium sp. strain was studied. it was found that a significant role in determination of cellulases is played by the adsorption of these enzymes on cellulose. the aeration level of the culture media had a significant effect on the synthesis as well as on the enzymatic complexity of cellulases.197770972
controlled cell disruption: a comparison of the forces required to disrupt different micro-organisms.a cell disrupter has been developed which can measure the forces required to disrupt both eukaryotic and prokaryotic cells. it operates a continuous process and will disrupt both large and small volumes. shear forces are set up when a suspension under laminar flow conditions is released under high pressure through a short orifice. if the applied pressure is altered, the shear forces are simultaneously changed so that the amount of cell disruption can be compared under different known and repeata ...1979422681
[antibacterial activity of zearalenone].zearalenone, a mycotoxin secreted by fusarium sp. and gibberella zeae, shows a narrow range of antibacterial activity limited to some gram-positive aerobic spore-forming bacteria. in bacillus thuringiensis (berliner), a highly sensitive species, this activity is characterized by a decrease of cellular division and induction of atypical cells. these effects resemble those obtained with two other mycotoxins which possess a lactone structure: aflatoxin b1 and patulin.1979455155
role of opportunistic fungi in ocular infections in nigeria.the importance of opportunistic fungal pathogens in causing ocular infections is emphasized. a study was conducted over a period of 4 years (1974--1977) to investigate the role of opportunistic fungi in causing mycotic keratitis and to elucidate certain aspects of epidemiology of this disease in nigeria. fifty-nine cases of corneal ulcers of suspected mycotic etiology were investigated. fungal etiology was confirmed in 42 of these cases. the predominant etiological agent was fusarium solani in 1 ...1978745623
a mycosis of the american lobster, homarus americanus, caused by fusarium sp. 19751167883
cutaneous involvement of systemic fungal infections in neutropenic patients.skin nodules can be the first evidence of a disseminated fungal infection in febrile neutropenic patients. we present our experience in the diagnosis of this clinical problem in five patients treated for serious hematologic conditions in our service. there were two cases of candida sp., two of fusarium sp., and one of trichosporon sp.. the immediate assessment of any suspicious lesion, including a biopsy of the lesion for microbiological and histopathologic examinations, will usually lead to the ...19921289191
[fungi isolated from diseased medicinal plants].one hundred and forty-four fungal isolates were obtained from diseased paeonia albiflora pall. var. trichocarpa bung., astragalus membranaceus bung., lithospermum erythrorhizon sieb. et zucc., ledebouriella seseloides wolff and bupleurum falcatum l. which were collected in the test field of tsukuba medicinal plant research station, national institute of hygienic sciences. most of them were identified into 15 genera containing 8 species. fungal species presumed to be pathogens of the host plants ...19921364438
beta-eliminative cleavage of the acidic polysaccharide of fusarium sp. m7-1 by an enzyme preparation of cellulomonas sp.by digestion with an enzyme preparation derived from a culture filtrate of cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and beta 1----2 mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of fusarium sp. m7-1 by acetolysis. the unsaturated disaccharide produced was isolated and identified as 4-deoxy-l-threo-hex-4-enopyranouronosyl alpha(1----2)-d-galactose. the same unsaturated sugar linked to the polysa ...19901368507
identification of o-alpha-d-glucopyranosyluronate-(1----2)-d-galactose isolated from the acidic polysaccharide of fusarium sp. m7-1. 19901368570
purification and characterization of a novel lyase from cellulomonas sp. that degrades fusarium and gibberella acidic polysaccharides.a cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of fusarium sp. m7-1 with the formation of mannose and o-beta-d-mannopyranosyl-(1----2)-d-mannose. deae-toyopearl 650m column chromatography showed three lyase activity peaks (fractions i, ii, and iii). the major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. the optimum ph was 6.5 to 8.0 and the stable ph range was 6.0 to ...19911368728
new cyclodepsipeptides, enniatins d, e and f produced by fusarium sp. fo-1305.new cyclodepsipeptides named enniatins d, e and f were isolated from the culture broth of fusarium sp. fo-1305 as inhibitors of acyl-coa:cholesterol acyltransferase (acat). the respective structures of enniatins d, e and f were determined to be cyclo[d-alpha-hydroxyisovaleryl(d-hiv)-l-n-methylleucinyl(l- me-leu)-d-hiv-l-n-methylvalinyl(l-me-val)-d-hiv-l-me-val], a mixture of cyclo-[d-hiv-l-me-leu-d-hiv-l-n-methylisoleucinyl(l-me-ile)-d-hiv- l-me-val] and cyclo(d-hiv-l-me-ile-d-hiv-l-me-leu-d-hiv ...19921399840
studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: iv. isolation and identification of four novel oligosaccharide units derived from the acidic polysaccharide chain.four novel oligosaccharide units were isolated from the acetolysis products of the acidic polysaccharide chain derived from the glycoproteins of fusarium sp. m7-1. their chemical structures were resolved mainly by 1h-nmr spectrometry in combination with methylation analysis and mass spectrometry. the results indicate that these oligosaccharide units originated from the side chains, glcnac alpha 1-->4glca alpha 1-->2(glcnac alpha 1-->4)glca alpha 1-->2gal, glcnac alpha 1-->4glca alpha 1-->2(glcna ...19921429523
occurrence of the mycotoxin chlamydosporol in fusarium species.the mycotoxin chlamydosporol (c11h14o5) has been independently isolated from three different fusarium species (f. chlamydosporum, f. acuminatum and f. culmorum), suggesting that its distribution may be widespread. we have developed a sensitive, specific fluorometric assay for chlamydosporol that allows quantitation in crude culture extracts. using this assay we have observed production of chlamydosporol on rice medium by 23 of 40 isolates (58%) of fusarium sp. examined in amounts as high as 0.8% ...19921440633
mycetoma of the foot due to fusarium sp. treated with oral ketoconazole.we report the case of a 30-year-old nigerian patient with mycetoma of the foot without bone involvement caused by fusarium sp..long-term administration of ketoconazole produced a significant improvement with good clinical and biological tolerance.19921498403
studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: i. isolation and some properties of the glycoproteins.an acidic heteropolysaccharide preparation derived from the mycelium of fusarium sp. m7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (cetavlon). these two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. two glycoproteins, precipitable gp i and nonprecipitable gp ii, were obtained. the molecular weights of gp i and gp ii were e ...19921569046
studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: ii. the primary structures of the low molecular weight carbohydrate chains of the glycoproteins.the primary structures of the o-glycosidically linked oligosaccharides isolated from glycoproteins gp i and gp ii of fusarium sp. m7-1 were established. the oligosaccharides released by alkaline borohydride treatment from the glycoproteins were purified by bio-gel p-4 and hplc. this approach resulted in one monosaccharide and seven oligosaccharides. their primary structures were resolved mainly by nmr spectrometry in combination with methylation mass spectrometry and fast atom bombardment mass s ...19921569047
studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: iii. the primary structures of the acidic polysaccharides of the glycoproteins.two o-glycosidically linked acidic polysaccharides, ap i and ap ii, were released, respectively, from glycoproteins gp i and gp ii of fusarium sp. m7-1 by alkaline borohydride treatment and purified by gel filtration chromatography. they were found to be apparently homogeneous on gel filtration chromatography and analytical ultracentrifugation. their molecular weights were estimated to be 8.2 x 10(4) and 3.1 x 10(4), respectively. the various oligosaccharide fragments obtained from ap i and ap i ...19921569048
[use of automated microscopic image analysis for the determination of microscopic hyphomycetes (fusarium sp.)].the method of automated microscopic image analysis was tested in intention to support the taxonomy of fusarium. the catalogue of morphometric object features of the software system amba provides size and shape parameters which can be utilize to discriminate populations of macroconidia of different fusarium species and varieties. the applied colouring of macroconidia (methylene blue) has no significant effect on these features. the results of statistical analysis show fitness of chosen technique.19901706549
suppurative keratitis in rural bangladesh: the value of gram stain in planning management.external eye disease which result in corneal scarring are an important cause of blindness in bangladesh and at the chittagong eye infirmary and training complex (eitc) over 200 cases of suppurative keratitis are managed each year. we reviewed the records of 127 cases of microbial keratitis to determine the relative contributions of gram stain and culture to diagnosis of the causative organism. there were 107 culture-proven cases of microbial keratitis amongst the 127 patients in this study. gram ...19911708747
influence of selenium on the efficiency of fungicide action against certain fungi.aspergillus funiculosus was isolated from rotted banana fruits, whereas alternaria tenuis and fusarium sp. were isolated from rotted tomato fruits. the isolated fungi tolerated relatively high levels of the fungicide, dithane, up to 2560 ppm on solid medium, but grew well at 40 ppm when supplemented with liquid medium. they are able to tolerate selenite up to 2% (w/v) sodium selenite. a. funiculosus showed no growth in the presence of mixture of 2.5 ppm selenium and 20 ppm dithane, whereas fusar ...19911711889
mycotic keratitis in paraguay.patients with corneal ulcer, residing mainly in rural areas of paraguay, were controlled for mycotic eye infections. in 26 cases out of 45 (58%) presence of filamentous fungi was observed (fusarium sp. (11), aspergillus sp. (5), cladosporium sp. (3), alternaria sp. (2), acremonium sp. (1), curvularia sp. (1), culture negative (3)). in 17 cases also pathogenic bacteria were present. most of the patients were farmers and belonged to the low income class. these data indicate that mycotic keratitis ...19911795722
[abnormalities of sexual development in puerto rico: status report].puerto rico presents the highest incidence in the world of anomalous sexual development. the authors have collected over 3100 cases in the past 19 years. clinical and laboratory studies suggest possible estrogenic contamination of meats and poultry products. variation in diet provides protection to a significant number of patients. the possibility of mycotic contamination of food employed in animal husbandry by fusarium sp., and mycotoxins capable of estrogenic effects have been suggested in a p ...19911817508
incidence of molds and mycotoxins in commercial animal feed mills in seven midwestern states, 1988-1989.a total of 82 feed manufacturers located within seven midwestern states (iowa, nebraska, minnesota, illinois, indiana, ohio, michigan) participated in a survey of mold and mycotoxin contamination of corn. samples were submitted from a composite of the grading samples taken from each incoming load of corn. the survey was initiated in july 1988. during the 12-mo period, moisture content of the corn samples upon receipt at the laboratory ranged from 10.5 to 13.3%. the greatest variation occurred in ...19911825995
[distribution studies of alpha-l-arabinofuranosidase already unstable at ph 7.0 (37 degrees c) in tumor-bearing mice in connection with its possible use to enhance the selectivity of the chemotherapy of malignant tumors].graffi et al. (1-3) had proposed the use of exogenous enzymes to toxify inactive transport forms of cancerostatic substances. for this purpose, the ph difference between normal tissues and the tumor was to be exploited, which can be essentially increased by the application of glucose and inorganic phosphate (5-7). earlier studies using alpha-l-arabinofuranosidase obtained from aspergillus niger have shown that the selectivity of tumor chemotherapy can be increased in this way (4). the alpha-l-ar ...19902369282
differentiation of medically important isolates of bipolaris and exserohilum with exoantigens.soluble culture extracts of bipolaris australiensis, b. hawaiiensis, b. spicifera, exserohilum longirostratum, e. mcginnisii, e. rostratum, and helminthosporium solani were used to prepare reference antisera in new zealand white rabbits. the absorbed reference antisera were tested by a microimmunodiffusion method against concentrated culture filtrates prepared from 115 environmental and clinical isolates of alternaria spp., bipolaris spp., curvularia spp., dactylaria sp., drechslera spp., embell ...19902380387
acid hydrolysis of beech sawdust hemicellulose and ethanol fermentation of hydrolysates by fusarium sp. 27.beech sawdust was subjected to autohydrolysis (200 degrees c) and acid hydrolysis in the presence of hcl and alcl3. hcl-catalyzed hydrolysis was favourable method to hydrolysis beech sawdust hemicellulose. the crude and pretreated hydrolysates were tested as substrates for ethanol production by fusarium sp. 27. reducing sugars were fermented to ethanol by the strain fusarium sp. 27 in yield 0.22 g ethanol per gram reducing sugars consumed.19852421542
selenium metabolism in a strain of fusarium.fusarium sp. was isolated from sinai soil at egypt. it showed tendency to tolerate high concentrations of selenium in the form of sodium selenite up to 3.5% (w/v). the microscopic examination revealed some morphological distortions. however, the fungus was capable to circumvent the toxic effect of selenium. the fungus possess strong reducing ability as high quantities of elemental selenium were precipitated within the fungal cells as well as on the surface of the fungal hyphae and spores. the pr ...19882484561
outbreak of trichothecene mycotoxicosis associated with consumption of mould-damaged wheat production in kashmir valley, india.during june to september, 1987, there were reports that a considerable segment of the population of kashmir valley, india, were affected by a gastrointestinal disorder. epidemiological investigations and laboratory based studies indicated that the outbreak was associated with the consumption of bread made from mould-damaged wheat. the disease was not age or sex specific. evidence of mould damage of wheat consisted of the presence of moulds (such as fusarium sp, aspergillus sp), and varying quant ...19892563012
effects of t-2 mycotoxin on gastrointestinal tissues: a review of in vivo and in vitro models.t-2 mycotoxin, a trichothecene, is the principal toxic component of fusarium sp. agricultural products and food are frequently contaminated with this toxin. various animal models have been used to determine its metabolic fate, rate of excretion, and distribution. a modulation effect on cell-mediated immunity and alterations in gastrointestinal propulsion have been demonstrated. the toxin has been shown to produce some similar pathologic alterations in various animal species studied. the consiste ...19892658861
dermatomycosis in dogs.during the routine examination of dogs for cutaneous lesions, 205 dogs were screened for fungi other than dermatophytes. twenty-two dogs (10.8%) revealed the presence of non-dermatophytic fungi suspicious for representing the etiologic agents of the skin lesions. the fungi isolated were alternaria sp. (2.9%), penicillium sp. (2.4%), aspergillus fumigatus (2.0%), mucor sp. (1.5%), cladosporium sp. (1.5%) and fusarium sp. (0.5%). no dermatophyte was isolated in association with these fungi. the in ...19892710156
toxicity of field samples and fusarium moniliforme from feed associated with equine-leucoencephalomalacia.feed samples of corn, pelleted feed, and oats associated with equine leucoencephalomalacia (elem) were obtained from north carolina, illinois, indiana, and oklahoma. these samples contained a high colony density of fusarium moniliforme sheldon which ranged from 64 to 87% of the total fungi. fifty-nine f. moniliforme strains were isolated. isolates of f. moniliforme in feed samples from illinois, indiana, and oklahoma were cultured on corn. the fermented corn, mixed with regular feed and fed to o ...19892730161
metabolic conversions of trichothecene mycotoxins: de-esterification reactions using cell-free extracts of fusarium.a crude cell-free extract from cultures of fusarium sp. strain c37410-90 possessed significant esterase activity and hydrolyzed the trichothecene mycotoxin 3-acetyldeoxynivalenol (3-acdon) to deoxynivalenol (don) in high yield. smaller amounts of 15-acetyl- and 3,15-diacetyl-esters of don were also formed. the extract was capable of hydrolyzing a range of natural and semisynthetic trichothecene esters, and showed a high degree of regioselectivity towards position 3. 3,4,15-triacetylscirpentriol ...19892775408
inhibition of hydroxymethylglutaryl-coenzyme a synthase by l-659,699.a beta-lactone isolated from fusarium sp. has been shown to be a potent specific inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) synthase [(s)-3-hydroxy-3-methylglutaryl-coa acetoacetyl-coa-lyase (coa-acetylating), ec, 4.1.3.5] from rat liver. the structure of this beta-lactone, termed l-659,699, is (e,e)-11-[3-(hydroxy-methyl)-4-oxo-2-oxytanyl]-3,5,7-trimethyl-2,4 - undecadienenoic acid. a partially purified preparation of cytoplasmic hmg-coa synthase from rat liver was ...19872890166
fusarium mycotoxins from peanuts suspected as a cause of sandhill crane mortality.an estimated 9,500 sandhill cranes (grus canadensis) died in gaines county, texas and roosevelt county, new mexico between 1982 and 1987. the predominant clinical sign observed in sick cranes was their inability to hold their heads erect, both while standing and flying. multiple muscle hemorrhages and submandibular edema were the most common lesions seen at necropsy. mycotoxins produced by fusarium sp. growing during cold, wet weather on peanuts left in the field after harvest, the predominant f ...19892915402
metabolic conversions of trichothecene mycotoxins: biotransformation of 3-acetyldeoxynivalenol into fusarenon-x.the trichothecene mycotoxin 3-acetyldeoxynivalenol was transformed by cultures of the diacetoxyscirpenol producer fusarium sp. strain c37410-90 into four compounds, identified as deoxynivalenol, 15-acetyldeoxynivalenol, 3,15-diacetyldeoxynivalenol and fusarenon-x. the major transformations are the result of specific esterification and de-esterification processes, but the production of fusarenon-x involves in addition a novel 4 beta-hydroxylation of the trichothecene ring system itself.19862948328
the isolation of fungi from laboratory dental pumice.samples of used dental laboratory pumice from the two dental laboratories were cultured for the isolation of fungi. the resulting supernatant fluid from sedimentation of each pumice sample after suspension in sterile saline was serially diluted and plated onto sabouraud agar. after incubation, fungal colonies observed were enumerated, isolated, and identified. the mean number of fungal colonies recovered from 10 pumice samples in laboratories i and ii was 51.0 x 10(2) and 22.6 x 10(2), respectiv ...19863021959
microorganisms capable of metabolizing the herbicide metolachlor.we screened several strains of microorganisms and microbial populations for their ability to mineralize or transform the herbicide metolachlor [2-chloro-n-(2-ethyl-6-methylphenyl)-n-(2-methoxy-1-methylethyl)-acetami de] because such cultures would potentially be useful in the cleanup of contaminated sites. although we used various inocula and enrichment culture techniques, we were not able to isolate microorganisms that could mineralize metolachlor. however, strains of bacillus circulans, bacill ...19873105457
seedborne fungi of sesame (sesamum indicum l) in sierra leone and their potential aflatoxin/mycotoxin production.sesame seeds (sesamum indicum l) from four different geographic locations in sierra leone were sampled for their mycoflora. three toxigenic aspergillus species: a. flavus link ex fries, a. ochraceus wilhelm, and a. tamarii kita were common to all samples. penicillium citrinum thom and two fusarium sp. were found in samples from two localities. the mycotoxins aflatoxin b1 and g1, ochratoxin a and b, and citrinin were positively identified.19883146700
[partial purification and properties of extracellular nadp+-dependent sorbitol dehydrogenase in fusarium sp]. 19883249587
disseminated cutaneous and peritoneal hyalohyphomycosis caused by fusarium species: three cases and review of the literature.three recent cases of hyalohyphomycosis caused by fusarium sp. illustrate differing aspects of infections produced by these organisms. one patient was undergoing continuous ambulatory peritoneal dialysis when peritonitis developed caused by fusarium moniliforme. removal of the catheter and amphotericin b were used in successful management. in a neutropenic patient on therapy for leukemia, multiple persistent infections occurred including jk diptheroids, and candida albicans sepsis. finally, nume ...19883278237
skin testing with extracts of fungal species derived from the homes of allergy clinic patients in toronto, canada.airborne fungi in the homes of patients with allergic rhinitis or asthma, from a toronto allergy clinic population, were isolated, quantified and identified to species. allergen extracts were prepared from sixteen of these isolated species and used for skin-prick testing of twenty-six patients. fourteen of the total patients reacted to one or more of these extracts at 1:10 (w/v) concentrations. the most common positive skin responses (8/14 to 6/14) were found for cladosporium cladosporioides, al ...19883349592
[epidemiologic study of mycetomas in mexico. apropos of 502 cases].the epidemiological data of 502 mycetomas studied in the department of mycology, "centro dermatológico pascua", mexico city, were analysed. mycetomas prevail in males (79.7%), they are more frequent between 16 and 45 years of age (75%) and among rural workers (62.5%); they preferentially affect lower limbs (62.5%); these data are generally similar to the known publications on the matter. actinomycetomas are the most frequent with 97.2% of the cases, distributed as follows: nocardia: 85.6% among ...19873621395
fusarin c production by north american isolates of fusarium moniliforme.a liquid culture medium was developed to screen north american isolates of fusarium moniliforme sheldon and fusarium subglutinans (wollenw. and reink.) nelson, toussoun, and marasas for their ability to produce fusarin c. parameters which were important for the optimal biosynthesis of fusarin c included ph (3.0 to 4.0), aeration, and sugar concentration (30 to 40%). of seven sugars tested, sucrose and glucose were the best carbohydrate sources for mycotoxin production, resulting in levels of fus ...19863954349
morphogenesis of fusarium sp-induced tibial dyschondroplasia in chickens.tibial dyschondroplasia was induced in female broiler chicks by the incorporation of 2% fusarium roseum "alaska" culture into their starter ration. chicks were placed on this diet at one day of age and maintained until they were killed at four days or one week. proximal tibial physes were grossly thickened into cone-shaped masses of cartilage by one week of age. microscopically, lesions were in both ages of treated chickens and were characterized by thickening of the transitional zone which was ...19854082389
ramihyphins--antifungal and morphogenic antibodies from fusarium sp. s-435. 19744435661
physiological properties and enzymatic activity of an arthrobacter capable of lysing fusarium sp. 19734723721
[biochemical aspects of fusarium poisoning--toxicological study of the substance synthesized by fusarium sp., gibberella saubinetti]. 19694896494
structural requirements of inducer for the formation of penicillin acylase in fusarium sp. 7.5-5. 19725085622
[keratomycosis. presentation of 3 cases caused by fusarium sp]. 19695787930
[nucleotides of microorganisms. xii. isolation and characterization of a new uridine nucleotide contained in fusarium sp]. 19666005039
conversion of o-coumaric acid to 4-hydroxycoumarin by fusarium sp. 19676082585
biosynthesis of cellulolytic enzymes in semi-continuous cultures.the aim of the research was to check the possibility of the semicontinuous cultivation of two fusarium sp. strains producing cellulolytic enzymes. the cultures were grown in 5 1 fermentor on the mineral medium with addition of 1% cellulose cotton. in these cultures lasting 9 to 16 days the total activity was about 13 mg of reducing sugar/ml culture filtrate/hour. the comparison of the results of semi-continuous and periodic cultures pointed to the productivity of cellulolytic enzymes which was 1 ...19816179397
enhanced cellulolytic enzyme biosynthesis of fusarium sp. strain by mutation and optimization of culture conditions.mutation and most favorable culture conditions resulted in 1.8 to 3.3-fold increase in beta-1, 4-endoglucanase and filter paper activity by fusarium sp. 25/2. culture fluid obtained after 3 days of growth of fusarium sp. mutant on modified saunders medium supplemented with 1% filter paper and 0.1% malt seedlings showed beta-1, 4-endoglucanase activity of 42.6 u/ml and fpa of 2.4 mg/0.5 ml x h.19836196946
[identification of the mycotoxin zearalenone in argentina].zearalenone, an estrogenic toxin produced by several fusarium sp., was detected by two chromatographic methods in grain and food samples from several farms of th buenos aires province. suspected zearalenone spots were identified by comparing their rf against that of the pure substance, by their change of behaviour under ultraviolet light of different wavelengths, by comparing the rf against a standard preparation after thin layer chromatography in four different solvent systems, and by color rea ...19806216499
estrogenic activity of zearalenone and zearalanol in the neonatal rat uterus.fusarium sp. contaminated feedstuffs elicit adverse estrogenic effects in several commercially important animal species via the mycotoxin zearalenone. an estrogenically active synthetic derivative, zearalanol, is used as an anabolic agent in cattle. since estrogens can irreversibly alter target tissue development, we investigated the estrogenic activity of these compounds in the neonatal rat uterus. both induced dose-dependent premature uterine growth when injected daily on postnatal days 1-5 (e ...19846235618
[disseminated infections caused by fusarium sp]. 19836635465
[degradation of a nylon polyamide fabric by fusarium sp]. 19836681232
an ultrastructural study of pale eumycetoma grains.the electron microscopic appearances of grains from seven patients with pale grain eumycetoma infections have been studied. the infecting fungi were pseudallescheria boydii (2), fusarium sp. (1), acremonium kiliense (1), a non-sporing mould (2) and trichophyton violaceum (1). p. boydii grown in vitro and grains from a madurella mycetomatis infection were used for comparison. the most important ultrastructural features of the pale eumycetoma grains were the gross thickening of the fungal cell wal ...19836686344
[effect of alanine and cysteine on the formation of biologically active substances by fusarium sp]. 19836855640
disseminated fusarium infection.a case of disseminated fusarium sp. infection in a bone-marrow transplant patient is described. the most likely source of the organism was food, and the importance of a specially prepared diet in immunosuppressed individuals is emphasized.19807007841
[mycosis of the foot in people over 40].some aspects of the ecology of the human foot in the stage of senescence have been studied. systematically 200 hospitalized patients, chosen at random, between the ages of 40 and 96 years were examined. the following was observed: 1.) 133 patients (66,5%) presented lesions suspected as dermatomycoses. 2.) 67 patients (33,5%) were free from clinical lesions. 42 (31,5%) positive direct examinations were obtained from the first group and 8 (11,8%) from the second one. in culture were isolated diffe ...19817022054
[pectolytic enzymes formed by penicillium and fusarium micromycetes].the ability of the two cultures penicillium sp. and fusarium sp. to synthesize extracellular pectolytic enzymes was investigated. the cultivation conditions providing a high level of the biosynthesis of these enzymes were identified. the methods of isolating the enzymes by means of organic solvents were developed. the pectinase from penicillium sp. showed a higher thermostability whereas that from fusarium sp. displayed a greater acid resistance. using glutaraldehyde and titanium salts, active i ...19827082428
fusarium infection in burned patients.the incidence of fungal infections of burn wounds is increasing because of the dramatic improvement in antibacterial chemotherapy and burn wound care. species of fusarium, common soil fungi and plant pathogens, are rarely isolated from burn wounds, and invasive disease due to these organisms is also rare. therefore, a case of burn wound infection with dissemination due to f. oxysporum is reported; three other cases of burn wound colonization by fusarium sp. are also reported. the literature on f ...19817211751
[molds in foods of the czechoslovak socialistic republik (author's transl)].the mycoflora of 13.527 g of food powders which were normal upon sensory evaluation as well of 22.017 pieces of surface sterilized grains and nuts was examined in the czechoslovak socialist republic. 147.027 colonies of 44 genera of molds were isolated and identified from samples. colonies of penicllium sp., aspergillus sp., and cladosporium sp. occured most often, less often or quite infrequently rhizopus sp., mucor sp., absidia sp., alternaria sp., circinella sp., trichoderma sp., botrytis sp. ...19817269803
cunninghamella bertholletiae wound infection of probable nosocomial origin.after closed reduction and casting of a tibial fracture in a 69-year-old diabetic man, a gangrenous lesion developed under the cast. cultures of debrided tissue revealed multiple bacteria, including bacillus sp, plus two fungi, fusarium sp and a zygomycete identified as cunninghamella bertholletiae. large nonseptate hyphae in thrombosed blood vessels suggested that c bertholletiae played a major role in the development of the infection. recovery of organisms from our patient's wound and from the ...19817280765
effect of fire on soil microorganisms in a meghalaya pine forest.the effect of man-made fire on soil microbial population in a natural subtropical pine forest eco-system, was studies. a nearly fifty years old pine (pinus kesiya royle) forest was cut and burnt in march 1977. another half of this pine forest was left uncut and unburnt. microbial population was destroyed completely just after the burning and recolonization occurred after some days. bacteria and actinomycetes were found to be the first colonizers followed by fungi. burning initiated better growth ...19817286854
cyclic dermatitis associated with fusarium sp infection in pinnipeds.dermatitis associated with fusarium sp infection developed in 3 california sea lions (zalophus californianus) and 3 gray seals (halichoerus grypus) at the national zoological park in washington dc. the lesions were papular or nodular and were distributed mainly on the face, trunk, and flippers. one sea lion died 6 weeks after extensive cutaneous involvement. the lesions regressed after 1 mild exacerabtion in the other 2 sea lions. in the gray seals, the skin condition appeared to worsen during t ...19817328003
nematodes associated with pawpaw (cv. homestead) in nsukka, nigeria.in field and greenhouse experiments, meloidogyne javanica caused symptomatic damage to pawpaw (cv. homestead). other important nematodes to which pawpaw seemed a good host included pratylenchus spp., rotylenchus reniformis, helioctylenchus spp., and tylenchulus martini. pawpaw appeared as a poor host to scutellonema clatbricaudatum and hoplolaimus pararobustus. fusarium sp. was isolated from the plant roots.19817342958
sexual fertility of forty fusarium strains from the european fusarium sambucinum project.fungus strains designated as fusarium sambucinum, f. torulosum, or fusarium sp. nov. were crossed with mat1-1 and mat1-2 tester strains of gibberella pulicaris. of the 40 field strains that were crossed with the tester strains, 13 strains produced fertile crosses and 27 strains did not produce fertile crosses. one strain designated as f. torulosum was fertile with a tester strain of g. pulicaris, suggesting that this is an intraspecies cross and that the strain is g. pulicaris, and, consequently ...19957566050
phylogenetic relationship within fusarium sambucinum fuckel sensu lato, determined from ribosomal rna sequences.partial ribosomal rna nucleotide sequences were determined for 11 strains of fusarium sambucinum fuckel sensu lato to assess by molecular genetic means, nirenberg's recent morphotaxonomic interpretation which split the species into three distinct taxa: f. sambucinum sensu stricto, f. torulosum, and one other species, as yet unnamed (fusarium species nova). four sequence patterns were identified among the 11 strains. two sequences that varied at one site were found among strains of f. sambucinum, ...19957566051
isolation and identification of novel acidic oligosaccharides derived from glycoproteins of fusarium sp. m7-1.four novel oligosaccharide chains were isolated from the glycoproteins of fusarium sp. m7-1. their chemical structures were resolved mainly by 400 mhz nmr spectrometry and mass spectrometry. the following structures were identified. man alpha 1-->2man-ol-6-p, man alpha 1-->2man alpha 1-->2man-ol-6-p, rha alpha 1-->2man alpha 1-->2man alpha 1-->2man-ol-6-p, and glcnac alpha 1-->4glca alpha 1-->2(glcnac alpha 1-->4)glca alpha 1-->2galf beta 1-->6(rha alpha 1-->2)man-ol.19957612994
cloning and nucleotide sequence of the alkaline protease gene from fusarium sp. s-19-5 and expression in saccharomyces cerevisiae.we have cloned a genomic dna encoding the alkaline protease (alp) of fusarium sp. s-19-5 from a genomic dna library and sequenced the nucleotides. complementary dna encoding alp was also isolated from the cdna library after amplifying the gene by pcr using partial sequences of the alp genomic dna as primers. the alp gene has an open reading frame of 1137 nucleotides containing three introns. a tata box (taaata) was observed 112 base pairs upstream from the translation initiation codon in the 5'- ...19947764853
high level expression of fusarium alkaline protease gene in acremonium chrysogenum.we transformed acremonium chrysogenum with the genomic dna of the alkaline protease (alp) from fusarium sp. s-19-5 including its promoter. most of the transformants thus obtained produced a large amount of alp. pcr and southern hybridization analysis of genomic dnas from these transformants showed chromosomal integration of the full-length alp gene. sds-page analysis of the supernatant from the transformants showed the presence of fusarium alp. the amino terminus of the alp produced in a. chryso ...19947764854
novel squalestatins produced by biotransformation.microorganisms were screened for the ability to modify the squalene synthase inhibitor squalestatin 1. biotransformation of 1 by two actinomycetes, s15106 and s15138, yielded three products hydroxylated on the 4,6-dimethyl-oct-2-enoyl side chain either at the 6 position (5) or 7 position (4 two diastereoisomers), and lacking the acetyl ester from the c-1 side chain. many strains were found to hydrolyse the 4,6-dimethyl-oct-2-enoyl or acetyl esters to yield squalestatins 2 or 3. the 3-methyl este ...19957775268
massive fungal contamination in animal care facilities traced to bedding supply.during the course of immunologic studies involving the gastrointestinal colonization of mice with candida albicans, it became apparent that the animals were being exposed to large numbers of aspergillus fumigatus spores which interfered with the c. albicans colonization. to determine the source of the a. fumigatus exposure and the extent of fungal contamination of the medical school vivarium and four satellite facilities, fungal analyses of feed, bedding, and air were undertaken. initial samples ...19957793950
rapid method for detection of aspergillus 5s ribosomal rna using a genus-specific oligonucleotide probe.ribosomal rna (rrna) is present in all prokaryotic and eukaryotic cells. although sequences are conserved, variations in nucleic acid composition are known to be species specific. formalin or bouin's fixed, paraffin-embedded tissue specimens from 17 cases of culture proven aspergillus sp infections were studied by a rapid (< 30 minutes) in situ hybridization procedure using a biotinylated oligonucleotide dna probe complementary to nucleic acids 1-22 of aspergillus sp 5s rrna sequence. positivity ...19957817944
isolation and structures of an antifungal antibiotic, fusarielin a, and related compounds produced by a fusarium sp.a new antifungal antibiotic, fusarielin a, and three related compounds, fusarielins b, c and d, were obtained from a culture of a fusarium sp. the skeletal structure and the relative stereochemistry of fusarielin a were determined mainly on the basis of its nmr data, and the absolute structure was elucidated by using the exciton chirality method and the modified mosher method. the structures of the other homologues were determined by comparison of their spectral data with those of fusarielin a.19957868388
suppurative corneal ulceration in bangladesh. a study of 142 cases examining the microbiological diagnosis, clinical and epidemiological features of bacterial and fungal keratitis.suppurative keratitis is an important preventable cause of blindness, particularly in the developing world. this study analyses 142 cases of suppurative keratitis referred to chittagong eye infirmary bangladesh. some 53.5% of cases were bacterial and 35.9% were fungal. the five most common pathogens were: pseudomonas sp. 24%, streptococcus pneumoniae 17%, aspergillus sp. 13%, fusarium sp. 7% and curvularia sp. 6%. gram stain and culture results were consistent in 62.6% of cases. previous antibio ...19947917262
prevalence of fungal corneal ulcers in northern india.fungal corneal ulcer is common in india due to the tropical climate and a large agrarian population that is at risk. various factors such as trauma, the injudicious use of topical antibiotics and corticosteroids are involved. many of the age and sex-related risk factors also play a minor role. this 6-year study from northern india revealed that fungi were detected in 61 (8.4%) out of 730 patients investigated. direct microscopy was positive in 51 (7%) and culture in 53 (7.3%) patients. aspergill ...19947927819
[fusarium sp. disseminated infection in a allogeneic bone marrow transplantation recipient]. 19947948120
the changing spectrum of fungal keratitis in south florida.to review the clinical experience with fungal keratitis in south florida over a 10-year period.19948008340
fusarium merismoides corda nr 6356, the source of the protein kinase c inhibitor, azepinostatin. taxonomy, yield improvement, fermentation and biological activity.fungal strain nr 6356, fusarium merismoides corda, was discovered as the source of the protein kinase c (pkc) inhibitor, azepinostatin. the strain was identified based on its growth on potato sucrose agar, slender conidial shape, characteristic polyphialide and production of abundant chlamydospores. fusarium aquaeductuum lagh. imi 103658 and fusarium sp. nr 7222 were also found to produce the same inhibitor. after single colony isolation and medium optimization trials, a more than 30-fold increa ...19948040068
clinical patterns of fusarium infections in immunocompromised patients.fusarium is an ubiquitous fungus commonly found in soil and on plants. human infection usually occurs as a result of inoculation of the organism through the body surface, thus causing skin infection, onychomycosis, keratitis, endophthalmitis and arthritis. dissemination may occur in subjects with underlying immunodeficiency. among immunocompromised hosts, fusarium sp. is an emerging pathogen in neutropenic patients. to our knowledge, since 1973, when the first disseminated fusariosis in a child ...19948077692
inhibition of beta-adrenergic binding by fungal metabolites.strains of aspergillus flavus, fusarium sp., rhizopus sp. and candida albicans all produced inhibitors of beta-adrenergic receptor binding; strains of saccharomyces sp. and schizosaccharomyces sp. did not. in tests with glutamic acid as the sole nutrient source, a fusarium sp. produced four-fold larger amounts of inhibitor than the other fungi. the inhibitor from the fusarium sp. was further purified by lyophilisation and sequential solvent extraction in chloroform, ethyl acetate and butanol; 60 ...19938093368
[problems found in the isolation of mycelial fungi (fusarium solani) from blood culture (bacter nr-860)].f. solani fungemia is unusual. patients at risk are immunosuppressed, have underlying malignancy or severe debilitating diseases.19938305557
disseminated cutaneous and vascular invasion by fusarium moniliforme in a fatal case of acute lymphocytic leukemia.a 35 year old female patient with acute lymphocytic leukemia developed fusariosis in which dissemination appeared to be limited to cutaneous and vascular invasion. the first evidence of fungemia occurred nearly seven months after initial hospitalization. the fungus was identified as fusarium sp. and was considered a contaminant. two weeks later blood cultures were again positive for fusarium sp. and the patient was placed on amphotericin b and 5-fluorocytosine therapy. the following day developi ...19938326994
disseminated visceral fusariosis treated with amphotericin b-phospholipid complex.fusariosis, a rare infectious disease of the immunocompromised host, is relatively resistant to amphotericin b (amb) or other antifungal agents. we describe a 5-year follow-up of a 40 year old woman with t-type acute lymphoblastic leukemia who following chemotherapy developed prolonged high fever, chills, night sweats, and severe weakness. liver function tests were impaired and abdominal computerized tomography (ct) showed multiple lesions in the liver and abnormal structure of the spleen. a lap ...19938348074
keratinophilic fungi in the antarctic environment.results of a study on the diffusion of keratinophilic fungi in an antarctic environment are given. nine soil samples collected from as many sites along the coast of ross sea, and six dust samples inside the italian scientific base were examined by direct inoculation and hair baiting methods for soil samples and plate dilution method for dust samples. as regards the variety of species isolated and the counting of the numbers of colonies, plate dilution method proved to be very effective. aspergil ...19938413499
purification and characterization of a new lipase from fusarium sp. ym-30.the extracellular lipase from fusarium sp. ym-30 was purified by a procedure involving ultrafiltration, ammonium sulfate precipitation, and deae-toyopearl 650m, cm-toyopearl 650m, and butyl-toyopearl 650m column chromatographies. the purified lipase was homogeneous with 12kda of molecular mass by sds-page, and had high specificities for mono- and diacylglycerols, but low toward triacylglycerols. the enzyme had maximum activity at ph 7.0 to 8.0 and 37 degrees c, and hydrolyzed digalactosyl diglyc ...19958520121
mycotoxins and fungi in wheat stored in elevators in the state of rio grande do sul, brazil.samples of wheat harvested from 1988 to 1990 and stored in elevators in the south of brazil (12 brazilian, 4 argentinian and 2 uruguayan) were analysed in 1990 for 14 mycotoxins: deoxynivalenol (don), nivalenol, diacetoxyscirpenol (das), t-2 and ht-2 toxins, t-2 triol, t-2 tetraol, aflatoxins b1, b2, g1, g2, ochratoxin a (ochra a), zearalenone and sterigmatocystin. one sample (1988 harvest) was contaminated with ochra a (0.04 microgram/g) and three other samples (1990 harvest) were contaminated ...19958522033
isolation and characterization of a novel endo-beta-galactofuranosidase from bacillus sp.a soil bacterium capable of growing on a polysaccharide-containing beta(1-->6)galactofuranoside residues derived from the acidic polysaccharide of fusarium sp. as a carbon source has been isolated. from various bacteriological characteristics, the organism was identified as a bacillus sp. the bacterium produced beta-galactofuranosidase inductively in the culture media. the most effective inducer for the beta-galactofuranosidase production was a polysaccharide containing beta(1-->5) or beta(1-->6 ...19958534975
[leukoencephalomalacia in two horses--moldy corn poisoning in germany?].moldy corn poisoning is a mycotoxicosis of fusarium sp. causing a disease termed equine leukoencephalomalacia (elem). this article reviews the literature on elem and describes two cases with clinical signs and morphological findings comparable with fusariotoxicosis. since in both cases neither a fungus nor a toxin proof were performed, the different causes of leukoencephalomalacia in horses are discussed.19958585077
[efficiency of microbank systems for the conservation of microorganisms relevant to veterinary medicine and others which are not easy to cultivate].in this paper the conservation of different bacteria, yeasts and molds from diagnostic material was examined with a commercial deep freeze system. 137 bacteria isolated from the stomachs of dogs, cats and pigs and from duodenal juice of cats and 7 isolates of yeasts and fungi from diagnostic material were conserved with the deep freeze system microbank-tm (mast diagnostica). furthermore 62 helicobacter pylori-isolates and 1 helicobacter felis-isolate were conserved with this system. after a stor ...19958593139
equine intestinal clostridiosis in a group of polo ponies in dubai, u.a.e.an acute outbreak of a c. perfringens type c enterotoxemia in a polo club killed 8 polo ponies in less than 24 hours. hay with a massive fungal contamination (aspergillus and fusarium sp.) is suspected to have triggered the enterotoxemia. our cases show numerous similarities with the previously reported cases of eic. however, acute toxic tubulonephrosis in the necropsied horses and longstanding elevation of ast and gamma gt-levels in survivors is unique in our cases.19968593152
degradation of beta 1-->6 galactofuranoside linkages in the polysaccharide of fusarium sp. m7-1 by endo-beta-galactofuranosidase from bacillus sp.a polysaccharide, in which the main part of the side chains were depleted, was prepared from the acidic polysaccharides of fusarium sp. m7-1 by digestion with lyase of cellulomonas sp. and mild acid treatment. this polysaccharide was degraded into several fragments, neutral oligosaccharides, neutral polysaccharide, and acidic polysaccharide, by an enzyme, endo-beta-galactofuranosidase, produced by bacillus sp. the main components of the oligosaccharides were isolated and identified as gal[sequen ...19968695912
fungal infections in neutropenic patients. a 8-year prospective study.in this paper we report a eight-year prospective study designed to further characterize incidence, epidemiology, specific syndromes, treatment and prognosis associated with fungal infections in neutropenic patients. during the study period 30 fungal infections were diagnosed in 30 patients among 313 episodes of fever and neutropenia (10%). there were 15 cases of candidiasis, 5 pulmonary aspergillosis, 3 sinusitis by aspergillus fumigatus, 5 infections by fusarium sp., one infection by trichospor ...19958729749
fatal hyalohyphomycosis following fusarium onychomycosis in an immunocompromised patient.a 35-year-old man with b lymphoblastic lymphoma was treated with bone marrow transplant and aggressive chemotherapy. he developed a fusarium infection of the great toenail. septicemic dissemination of a fusarium sp. occurred 9 months later during a lymphoma relapse. the clinical course of the hyalohyphomycosis was then rapidly fatal despite institution of amphotericin b therapy.19968739996
disseminated fusarium sp. infection affecting the brain of a child after bone marrow transplantation.a case of fusarium sp. infection of the brain in a 6-year-old child who underwent allogeneic bmt is reported. as far as the authors know, this is the first report of fusarium sp. encephalitis in a bmt patient. fusarium sp. infection is a rare but emerging fungal pathogen after bmt and, because of several similarities, it is often mistaken for other mold infections, such as aspergillus sp. the importance of early identification of this fungus as a cause of disseminated fungal infection in bmt pat ...19968932859
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