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catechin content of 18 teas and a green tea extract supplement correlates with the antioxidant capacity.our literature review of currently available data in the area of tea and cancer prevention demonstrated that there is more conclusive evidence for the chemopreventive effect of green tea compared with black tea. we suggest that this is due to a large variation of the flavanol content in tea, which is not taken into consideration in most of the epidemiological studies. it was the purpose of this study to determine the flavanol content of various teas and tea products and to correlate it with thei ...200312881018
black tea prevents cigarette smoke-induced oxidative damage of proteins in guinea pigs.cigarette smoke (cs) causes oxidative damage and tea polyphenols have strong antioxidant properties. therefore, we studied the effect of a black tea (bt) infusion on cs-induced oxidative damage of proteins both in vitro and in vivo. in the in vitro experiment, bovine serum albumin (bsa) or a guinea pig tissue microsomal suspension was incubated with an aqueous extract of cs (cs-solution) in the presence or absence of the bt infusion. protein oxidation was measured by immunoblotting of the dinitr ...200312888648
black tea extract supplementation decreases oxidative damage in jurkat t cells.the purpose of this study was to investigate the protective effect of black tea (bt) extract against induced oxidative damage in jurkat t-cell line. cells supplemented with 10 or 25 mg/l bt were subjected to oxidation with ferrous ions. malondialdehyde (mda) production as marker of lipid peroxidation, dna single strand breaks as marker of dna damage, and modification of the antioxidant enzyme activity, glutathione peroxidase (gpx) were measured. results show the efficacy of bt polyphenols to dec ...200312893297
aluminium and fluoride concentrations of three tea varieties growing at lantau island, hong kong.the present project aims to investigate aluminium (al) and fluoride (f) contents in teas (camellia sinensis (l.) o. kuntze). three different commercial tea varieties: assam variety and two china sub-varieties, a large leafed variety and small leafed variety, were collected in two tea gardens of lantau island tea plantation of hong kong. in general, high concentrations of al and f were accumulated in the mature leaves (15.3 and of 2.07 g kg-1 respectively). among the three varieties, 'the small l ...200312901167
lack of association between tea and cardiovascular disease in college alumni.epidemiological studies suggest that tea intake, a major dietary source of flavonoids, may be associated with a decreased risk of cardiovascular disease (cvd).200312913023
screening of chemopreventive tea polyphenols against pah genotoxicity in breast cancer cells by a xre-luciferase reporter construct.polycyclic aromatic hydrocarbons (pahs) are established cancer initiators that can be found in our food and environment. some dietary plant polyphenols are strong inhibitors to pah-induced mutagenesis, whereas others may not be as effective. to identify the chemopreventive compounds from a huge volume of dietary components, the development of an efficient screening method is required. in this study, a xenobiotic response element (xre)-luciferase reporter plasmid was constructed to screen for som ...200312925309
identification of chlorophylls and carotenoids in major teas by high-performance liquid chromatography with photodiode array detection.the separation and identification of pigments, chlorophylls, and carotenoids of seven teas and fresh leaf of tea (camellia sinensis) by high-performance liquid chromatography (hplc) are described. hplc was carried out using a symmetry c(8) column with a photodiode array detector. pigments were eluted with a binary gradient of aqueous pyridine solution at a flow rate of 1.0 ml/min at 25 degrees c. hplc analyses achieved the separation of more than 100 pigment peaks, and 79 pigment species, 41 chl ...200312926875
suppression of fatty acid synthase in mcf-7 breast cancer cells by tea and tea polyphenols: a possible mechanism for their hypolipidemic effects.tea is a heavily consumed beverage world wide because of its unique aroma, less cost and broad availability. fatty acid synthase (fas) is a key enzyme in lipogenesis. fas is overexpressed in the malignant human breast carcinoma mcf-7 cells and its expression is further enhanced by the epidermal growth factor (egf). the egf-induced expression of fas was inhibited by green and black tea extracts. the expression of fas was also suppressed by the tea polyphenol (-)-epigallocatechin 3-gallate (egcg), ...200312931129
thoughts on thearubigins.the chemistry underlying the changes which occur during tea leaf fermentation is reviewed and used as a basis for proposals for the structure of thearubigins, the major pigments of black teas.200312946406
chain-breaking antioxidant activity and cyclic voltammetry characterization of polyphenols in a range of green, oolong, and black teas.a series of eight green, eight oolong, and 17 black teas have been analyzed for polyphenol content by absorbance at 272 nm and cyclic voltammetry response at an inert carbon electrode, a new method developed to provide a rapid measure of easily oxidizable polyphenols in beverages. the chain-breaking antioxidant activity of the teas has also been determined during the chain oxidation of methyl linoleate in a ph 7.4 micellar solution, for which realistic kinetic parameters have been derived. while ...200312952436
the prevalence of tongue lesions in 5150 turkish dental outpatients.the aim of this study was to assess the prevalence of nine tongue conditions and relate to data obtained about oral hygiene or habits in turkish dental outpatients.200312974518
adsorption from black tea and red wine onto in vitro salivary pellicles studied by ellipsometry.the adsorption of black tea and red wine components onto a pellicle-like protein layer formed in vitro by adsorption from whole unstimulated saliva on hydroxyapatite discs were studied by in situ ellipsometry. it was found that components from black tea readily adsorbed to the pellicle. subsequent exposure to saliva led to further adsorption of salivary components to give an overall increase in the amounts adsorbed. the amounts adsorbed increased still further following a third tea and saliva ex ...200312974686
[saponins from the seeds of thea sinensis l. iii. the constitution of thea-sapogenol. (1)]. 195413215030
hydroxyl radical and hypochlorous acid scavenging activity of small centaury (centaurium erythraea) infusion. a comparative study with green tea (camellia sinensis).small centaury (centaurium erythraea rafin.) is a herbal species with a long use in traditional medicine due to its digestive, stomachic, tonic, depurative, sedative and antipyretic properties. this species is reported to contain considerable amounts of polyphenolic compounds, namely xanthones and phenolic acids as the main constituents. although the antiradicalar activity of some pure polyphenolic compounds is already known, it remains unclear how a complex mixture obtained from plant extracts ...200313678237
coordination of aluminium with purpurogallin and theaflavin digallate.polyphenols are antioxidants, which are known to influence bioavailability of metals in the body. the theaflavins of black tea are important members of this family, which have been sparsely investigated. the complexation of aluminium with purpurogallin (2,3,4,6-tetrahydroxy-5h-benzocyclohepten-5-one) has been investigated using nuclear magnetic resonance (nmr) spectroscopy, liquid chromatography-mass spectroscopy (lc-ms) and fourier transform infrared (ft-ir) spectroscopy. 1h nmr was used to det ...200313678812
notes on fungi from north-east india. x. chaetospermum camelliae sp. nov. on tea (camellia sinensis (l.) o. kuntze). 196213859604
antimutagenic effects of black tea in the salmonella typhimurium reverse mutation assay.black tea (camellia sinensis) is one of the most widely consumed beverages worldwide. its chemopreventive effects are well documented in the literature. in the present set of investigations antimutagenic effects of aqueous black tea extract (ate) and black tea polyphenols (btp) were evaluated in the ames test using salmonella typhimurium tester strains ta 98 and ta 100. addition of benzo(a)pyrene (bap) and cyclophosphamide (cp), two well known mutagens, at the concentrations of 20 and 15 microg/ ...200314507238
modulation of arsenic induced cytotoxicity by tea.arsenic, a naturally ocurring chemical element, is considered hazardous to human health. inorganic arsenic compounds were found to induce cytotoxicity in chinese hamster v-79 cells in culture. the arsenite form was more toxic than arsenate. extracts of green and two varieties of black tea, as well as their principal polyphenols, (-)-epigallocatechingallate and theaflavin, efficiently counteracted the cytotoxic effects of arsenic compounds. on the basis of the amount of tea extract that afforded ...200314507244
mechanisms of cancer prevention by tea constituents.consumption of tea (camellia sinensis) has been suggested to prevent cancer, heart disease and other diseases. animal studies have shown that tea and tea constituents inhibit carcinogenesis of the skin, lung, oral cavity, esophagus, stomach, liver, prostate and other organs. in some studies, the inhibition correlated with an increase in tumor cell apoptosis and a decrease in cell proliferation. studies with human cancer cell lines have demonstrated that epigallocatechin-3-gallate (egcg), a major ...200314519824
antioxidant activity of tea polyphenols in vivo: evidence from animal studies.tea is particularly rich in polyphenols, including catechins, theaflavins and thearubigins, which are thought to contribute to the health benefits of tea. tea polyphenols act as antioxidants in vitro by scavenging reactive oxygen and nitrogen species and chelating redox-active transition metal ions. they may also function indirectly as antioxidants through 1) inhibition of the redox-sensitive transcription factors, nuclear factor-kappab and activator protein-1; 2) inhibition of "pro-oxidant" enz ...200314519826
antioxidant effects of tea: evidence from human clinical trials.tea remains the most consumed drink in the world after water, well ahead of coffee, beer, wine and carbonated soft drinks. an accumulated number of population studies suggests that consumption of green and black tea beverages may bring positive health effects (1). one hypothesis explaining such effects is that the high levels of flavonoids in tea can protect cells and tissues from oxidative damage by scavenging oxygen-free radicals. chemically, the flavonoids found in green and black tea are ver ...200314519827
black tea consumption reduces total and ldl cholesterol in mildly hypercholesterolemic adults.despite epidemiological evidence that tea consumption is associated with the reduced risk of coronary heart disease, experimental studies designed to show that tea affects oxidative stress or blood cholesterol concentration have been unsuccessful. we assessed the effects of black tea consumption on lipid and lipoprotein concentrations in mildly hypercholesterolemic adults. tea and other beverages were included in a carefully controlled weight-maintaining diet. five servings/d of tea were compare ...200314519829
effect of increased tea consumption on oxidative dna damage among smokers: a randomized controlled study.tea drinking has been associated with decreased occurrence of cancer and heart disease. one potential mechanism for these findings is the strong antioxidant effect of tea polyphenols. a phase ii randomized controlled tea intervention trial was designed to study the effect of high consumption (4 cups/d) of decaffeinated green or black tea on oxidative dna damage as measured by urinary 8-hydroxydeoxyguanosine (8-ohdg) among smokers over a 4-mo period. a total of 143 heavy smokers, aged 18-79 y, we ...200314519830
the epidemiology of tea consumption and colorectal cancer incidence.this manuscript provides a brief synopsis of 30 studies aimed at examining tea consumption as a factor in the incidence of colon and rectal cancers. the 30 papers examine populations in 12 countries and provide data on consumption of both black and green tea. these studies do not provide consistent evidence to support the theory from animal studies and basic research that tea is a potent chemopreventive agent. details of the studies are presented, and the potential impact of measurement error, p ...200314519831
fermentation characteristics of some assamica clones and process optimization of black tea manufacturing.changes in the specific activities of polyphenol oxidase (ppo), peroxidase (pod), and protease and in the relative amounts of flavan-3-ols for eight genetically derived cultivated teas at various stages of leaf maturity and in four succescive seasons were examined. a series of investigations were carried out to study the cross-reactivity of complex polyphenols and ppo-generated orange-yellow theaflavins, as well as of pod oxidized substrates, producing brown so-called thearubigins during ferment ...200314558781
catechin attenuates 6-hydroxydopamine (6-ohda)-induced cell death in primary cultures of mesencephalic cells.past studies have shown the protective effects of tea catechins on oxidative cell damage induced by 6-ohda in pc12 cells. in this study we verified whether or not catechin prevents 6-ohda-induced oxidative cell damage in primary cultures of rat mesencephalic cells. on exposure to 6-ohda (200 microm), the cultures showed a marked decrease in cell viability, disturbances in lipid peroxidation, and an increased generation of no, as assayed by mtt, tbars and nitrite assays, respectively. introductio ...200314559299
[antioxidant activities of green and black teas determined by the cumene hydroperoxide/hemoglobin.methylene blue method].antioxidant activity in tea was measured by the new cumene hydroperoxide/hemoglobin.methylene blue(chp/hb.mb) method developed in our laboratory. using the chp/hb.mb method, we investigated the activities of polyphenols(11 varieties) in order to determine their reactivity on chp. according to the chp/hb.mb method, an increase in the number of hydroxyl groups in polyphenols induced high antioxidant activity. we found that this method was capable of measuring the antioxidant activity of polyphenol ...200314560653
effective use of tea to limit dietary iron available to starlings (sturnus vulgaris).wild-caught starlings (sturnus vulgaris) were fed an iron-enriched diet, with or without supplemental black tea leaves, to determine whether tea-derived tannins would prevent intestinal iron absorption. hepatic biopsies were obtained to determine hepatic iron concentrations by atomic absorption spectroscopy. hepatic iron concentrations increased significantly (p = 0.04) in 21 birds that consumed only the iron-enriched diet for 6 mo but not in the 20 birds that consumed the iron-enriched diet wit ...200314582799
molecular detection of cashew husk (anacardium occidentale) adulteration in market samples of dry tea (camellia sinensis).species-specific pcr primers were developed from intergenic spacer regions of 5s ribosomal rna genes and used successfully in the detection of adulteration of cashew husk (anacardium occidentale l.) in tea [camellia sinensis (l.) o. kuntze] samples. this is the first report of detecting adulteration in tea using molecular tools. application of this approach in detecting adulteration of other biological materials in tea, medicinal herbs and the composition of admixtures of ayurvedic herbs has bee ...200314598225
green tea polyphenol epigallocatechin-3-gallate (egcg) differentially inhibits interleukin-1 beta-induced expression of matrix metalloproteinase-1 and -13 in human chondrocytes.interleukin-1beta (il-1beta)-induced inflammatory response in arthritic joints include the enhanced expression and activity of matrix metalloproteinases (mmps), and their matrix degrading activity contribute to the irreversible loss of cartilage and may also be associated with sustained chronic inflammation. we have earlier shown that green tea (camellia sinensis) polyphenol epigallocatechin-3-gallate (egcg) was non-toxic to human chondrocytes [singh r, ahmed s, islam n, goldberg vm, and haqqi t ...200414600251
tea intake, comt genotype, and breast cancer in asian-american women.there is substantial in vitro and in vivo evidence implicating tea polyphenols as chemopreventive agents against various cancers. in a case-control study conducted among asian-american women in los angeles county, we reported a significant inverse relationship between intake of green tea and risk of breast cancer (a. h. wu et al., int. j. cancer, 106: 574-579, 2003). because catechol-containing tea polyphenols are very rapidly o-methylated by human catechol-o-methyltransferase (comt), we are int ...200314612555
combined inhibition of estrogen-dependent human breast carcinoma by soy and tea bioactive components in mice.breast cancer is significantly less prevalent among asian women, whose diets contain high intake of soy products and tea. the objective of our present study was to identify the combined effects of dietary soy phytochemicals and tea components on breast tumor progression in a clinically relevant in vivo model of mcf-7 androgen-dependent human breast tumor in female scid mice. mcf-7 tumor growth, tumor cell proliferation and apoptosis, microvessel density, and expressions of tumor estrogen recepto ...200414618609
tea polyphenols regulate nicotinamide adenine dinucleotide phosphate oxidase subunit expression and ameliorate angiotensin ii-induced hyperpermeability in endothelial cells.out-of-control reactive oxygen species (ros) signaling is one of the key events in the pathogenesis of endothelial dysfunction and essential hypertension. we observed that tea polyphenols decreased the production of ros via regulation of the protein expression of nicotinamide adenine dinucleotide phosphate (nadph) oxidase in bovine carotid artery endothelial cells (bcaecs). both green tea polyphenols (gtp) and black tea polyphenols (btp) down-regulated the expression of nadph oxidase subunits p2 ...200314621186
[liver toxicity of camellia sinensis dried etanolic extract]. 200314622530
inhibition of caffeine biosynthesis in tea (camellia sinensis) and coffee (coffea arabica) plants by ribavirin.the effects of ribavirin, an inhibitor of inosine-5'-monophosphate (imp) dehydrogenase, on [8-(14)c]inosine metabolism in tea leaves, coffee leaves and coffee fruits were investigated. incorporation of radioactivity from [8-(14)c]inosine into purine alkaloids, such as theobromine and caffeine, guanine residues of rna, and co(2) was reduced by ribavirin, while incorporation into nucleotides, including imp and adenine residues of rna, was increased. the results indicate that inhibition of imp dehy ...200314623114
cocoa has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine.black tea, green tea, red wine, and cocoa are high in phenolic phytochemicals, among which theaflavin, epigallocatechin gallate, resveratrol, and procyanidin, respectively, have been extensively investigated due to their possible role as chemopreventive agents based on their antioxidant capacities. the present study compared the phenolic and flavonoid contents and total antioxidant capacities of cocoa, black tea, green tea, and red wine. cocoa contained much higher levels of total phenolics (611 ...200314640573
antioxidative activities of volatile extracts from green tea, oolong tea, and black tea.antioxidative activities of volatile extracts from six teas (one green tea, one oolong tea, one roasted green tea, and three black teas) were investigated using an aldehyde/carboxylic acid assay and a conjugated diene assay. the samples were tested at levels of 20, 50, 100, and 200 micrograms/ml of dichloromethane. the results obtained from the two assays were consistent. all extracts except roasted green tea exhibited dose-dependent inhibitory activity in the aldehyde/carboxylic acid assay. a v ...200314640590
unfermented rooibos tea: quantitative characterization of flavonoids by hplc-uv and determination of the total antioxidant activity.unfermented rooibos originates from the leaves and the stems of the indigenous south african plant, aspalathus linearis, and it has been reported to have a higher content of flavonoids compared to that of fermented rooibos. the hplc/uv method developed in our laboratory for the analysis of the fermented rooibos was applied to the quantitative characterization of the major flavonoids present in the unfermented rooibos. main compounds determined were aspalathin (49.92 +/- 0.80 mg/g), isoorientin ( ...200314640601
the impact of ph and calcium on the uptake of fluoride by tea plants (camellia sinensis l.).tea plants (camellia sinensis l.) accumulate large amounts of fluoride (f) from soils containing normal f concentrations. the present experiments examined the effects of ph and ca on f uptake by this accumulating plant species.200414644914
epigallocatechin-3-gallate is absorbed but extensively glucuronidated following oral administration to mice.epigallocatechin-3-gallate (egcg), the most abundant catechin in green tea (camellia sinensis), has shown cancer preventive activity in animal models. the bioavailability of egcg in the most commonly used animal species, mice, is poorly understood. moreover, the pharmacokinetic parameters of egcg have not been reported previously in mice. here we report that after administration of egcg intravenously at 21.8 micro mol/kg or intragastrically at 163.8 micro mol/kg, the peak plasma levels of egcg i ...200314652367
influence of milk on the antimutagenic potential of green and black teas.the objective of this study was to evaluate whether addition of milk to green, black and decaffeinated black teas alters their antimutagenic activity.201614666689
foods and beverages in relation to urothelial cancer: case-control study in japan.the roles of several foods and beverages in the development of bladder cancer remain unclear.200414678179
cancer prevention by tea polyphenols is linked to their direct inhibition of antiapoptotic bcl-2-family proteins.epidemiological data and in vitro studies on cancer chemoprevention by tea polyphenols have gained attention recently from the scientific community, nutritionists, the pharmaceutical industry, and the public. despite the several efforts made recently to elucidate the molecular basis for the anticancer activity of these natural products, little correlation has been found thus far between the putative protein targets of compounds found in tea extracts and levels found in plasma after tea consumpti ...200314678963
modulation of hepatic drug metabolizing enzymes and oxidative status by rooibos (aspalathus linearis) and honeybush (cyclopia intermedia), green and black (camellia sinensis) teas in rats.rooibos and honeybush teas significantly (p < 0.05) enhanced the activity of cytosolic glutathione s-transferase alpha. a significant (p < 0.05) to marginal (p < 0.1) increase in the activity of the microsomal udp-glucuronosyl transferase was obtained with unprocessed rooibos and honeybush teas, respectively. oxidized glutathione (gssg) levels were significantly (p < 0.05) reduced in the liver of all tea treated rats while reduced glutathione (gsh) was markedly increased in the liver of the herb ...200314690405
anti-trypanosoma cruzi activity of green tea (camellia sinensis) catechins.the trypanocidal action of green tea catechins against two different developmental stages of trypanosoma cruzi is reported for the first time. this activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. an ethyl acetate fraction from camellia sinensis green tea leaves, which contains most of the polyphenolic compounds and the maximal trypanocidal activity, was obtained by fractionation of the aqueous extract wit ...200414693520
black and green tea polyphenols attenuate blood pressure increases in stroke-prone spontaneously hypertensive rats.oxidative stress was reported to be involved not only in cardiovascular diseases, but also in hypertension. epidemiologic studies indicated that tea consumption slightly reduces blood pressure. we conducted two studies to determine whether black and green tea can lower blood pressure (bp) in stroke-prone spontaneously hypertensive rats (shrsp). male shrsp (n=15) were allowed to recover for 2 wk after a transmitter for measuring bp was implanted in the peritoneal cavity. the rats were divided int ...200414704290
impact of tea drinking on iron status in the uk: a review.the aims of this review are (1) to evaluate the literature on the likely impact of tea drinking on the iron status of different groups within the uk population and (2) to formulate targeted and evidence based advice on tea drinking in the context of iron nutrition in different groups of people.200414718031
production of hydrogen peroxide by polyphenols and polyphenol-rich beverages under quasi-physiological conditions.to investigate the ability of the production of h(2)o(2) by polyphenols, we incubated various phenolic compounds and natural polyphenols under a quasi-physiological ph and temperature (ph 7.4, 37 degrees c), and then measured the formation of h(2)o(2) by the ferrous ion oxidation-xylenol orange assay. pyrocatechol, hydroquinone, pyrogallol, 1,2,4-benzenetriol, and polyphenols such as catechins yielded a significant amount of h(2)o(2). we also examined the effects of a metal chelator, ph, and o(2 ...200314730143
tissue distribution and intracellular localization of catechins in tea leaves.we investigated the leaf tissue and cellular morphology of tea (camellia sinensis). osmiophilic material, presumably catechins, was present in mesophyll cells, but not in epidermal cells. electron microscopy showed that catechins were localized to restricted regions within the central vacuoles. in addition, two kinds of small vacuoles of 0.5-3 microm were present in mesophyll cells. one vacuole had catechins within its whole lumen, while the other had an electron-lucent lumen. we found fusion pr ...200314730155
modulation of phosphatidylinositol-3-kinase/protein kinase b- and mitogen-activated protein kinase-pathways by tea polyphenols in human prostate cancer cells.we have earlier shown that oral infusion of a polyphenolic fraction isolated from green tea, at a human achievable dose (equivalent to six cups of green tea per day), significantly inhibits prostate cancer (pca) development and metastasis in transgenic adenocarcinoma of mouse prostate (tramp) model that closely mimics progressive form of human prostatic disease (gupta et al. [2001]: proc. natl. acad. sci. u.s.a. 98:10350-10355.). a complete understanding of the mechanism(s) and molecular targets ...200414743383
delivery of tea polyphenols to the oral cavity by green tea leaves and black tea extract.catechins and theaflavins, polyphenolic compounds derived from tea (camellia sinensis, fam. theaceae), have been reported to have a wide range of biological activities including prevention of tooth decay and oral cancer. the present study was undertaken to determine the usefulness of green tea leaves and black tea extract for the delivery of catechins and theaflavins to the oral cavity. after holding either green tea leaves (2 g) or brewed black tea (2 g of black tea leaves in 100 ml) in the mou ...200414744744
effects of a controlled diet and black tea drinking on the fecal microflora composition and the fecal bile acid profile of human volunteers in a double-blinded randomized feeding study.although diet has been clearly associated with human health many potential mechanisms remain undefined. for instance, although the intestinal bacterial microflora has long been postulated to contribute to human health, little is known about the effects of diet on the bacterial microflora composition and the specific contributions of the microflora to human health. thus, we analyzed 1) changes in the fecal microflora composition by fluorescent in-situ hybridization (fish) and denaturing gradient ...200414747691
phenolic acid metabolites as biomarkers for tea- and coffee-derived polyphenol exposure in human subjects.tea and coffee are rich in polyphenols with a variety of biological activities. many of the demonstrated activities are consistent with favourable effects on the risk of chronic diseases. 4-o-methylgallic acid (4omga) and isoferulic acid are potential biomarkers of exposure to polyphenols derived from tea and coffee respectively. 4omga is derived from gallic acid in tea, and isoferulic acid is derived from chlorogenic acid in coffee. our major objective was to explore the relationships of tea an ...200414756917
comparison of cytotoxicity and radical scavenging activity between tea extracts and chinese medicines.three hot water extracts of black tea, green tea and powdered green tea and five chinese medicines (shosaiko-tou, orengedoku-tou, goshuyu-tou, choto-san, keishininjinn-tou) were investigated for their ability to modify nitric oxide (no) production by lipopolysaccharide (lps)-stimulated mouse macrophage-like raw 264.7 cells, and for their cytotoxicity, radical intensity and scavenging activity. all eight materials significantly reduced the extracellular concentration of no in the lps-stimulated r ...200314758724
anti-inflammatory activity of tea (camellia sinensis) root extract.pharmacological studies were carried out with methanol-water (1:1) extract of dried tea (camellia sinensis) root extract (tre). tre was found to possess anti-inflammatory, analgesic and antipyretic activities at 1/10th of its ld50 dose of 100 mg/kg i.p. it was found that tre inhibited the arachidonic acid-induced paw oedema in rats which indicated that tre produced the anti-inflammatory activity by inhibiting both the cyclooxygenase and lypooxygenase pathways of arachidonic acid metabolism. tre ...200414761665
comparative study of the growth-inhibitory and apoptosis-inducing activities of black tea theaflavins and green tea catechin on murine myeloid leukemia cells.among the black tea polyphenols, theaflavins are generally considered to be the more effective components for the inhibition of carcinogenesis. in this study, we attempted to compare the growth-inhibitory and apoptosis-inducing activities of the four black tea theaflavins (tf-1, tf-2a, tf-2b and tf-3) with the major green tea catechin epigallocatechin-3-gallate (egcg) on the murine myeloid leukemia wehi-3b jcs cells. all the four black tea theaflavins were shown to exert potent anti-proliferativ ...200414767581
[acute hepatitis due to exolise, a camellia sinensis-derived drug]. 200314770123
fluoride and aluminum in teas and tea-based beverages.to evaluate fluoride and aluminum concentration in herbal, black, ready-to-drink, and imported teas available in brazil considering the risks fluoride and aluminum pose to oral and general health, respectively.200414963548
inhibitory effects on phospholipase a2 and antivenin activity of melanin extracted from thea sinensis linn.antivenin activity of melanin extracted from black tea (mebt) was reported for the first time. the antagonistic effect of mebt was evaluated for agkistrodon contortrix laticinctus (broadbanded copperhead), agkistrodon halys blomhoffii (japanese mamushi), and crotalus atrox (western diamondback rattlesnake) snake venoms administered i.p. to icr mice. mebt was injected i.p. immediately after the venom administration in dose of 3 mg per mouse in the same place of venom injection. mebt demonstrated ...200414967198
iron bioavailability and weight gains to iron-deficient rats fed a commonly consumed tunisian meal 'bean seeds ragout' with or without beef and with green or black tea decoction.the fe bioavailability and the weight gains were evaluated in rats fed a commonly consumed tunisian meal 'bean seeds ragout' (bsr), with or without beef and with black or green tea decoction. the fe bioavailability was evaluated in fe-deficient rats by the hemoglobin repletion method and the fe stored in the liver. the addition of beef to the bsr significantly increased the fe bioavailability from this meal by 147% and the reserve of fe stored in the liver by 77% (p < 0.001). in contrast, both b ...200314968927
metabolite profiling using (1)h nmr spectroscopy for quality assessment of green tea, camellia sinensis (l.).a set of 191 green teas from different countries was collected and analyzed by (1)h nmr. it was proposed to establish if the teas could be discriminated according to the country of origin or with respect to quality. both principal component analysis (pca) and cluster analysis were applied to the data. some separation of chinese and non-chinese teas was observed. the present results did not allow allocation of samples to individual countries, but cluster analysis suggested that it might be possib ...200414969518
characterization of corrinoid compounds from a japanese black tea (batabata-cha) fermented by bacteria.a japanese fermented black tea (batabata-cha) contained a considerable amount of vitamin b(12) (456 +/- 39 ng per 100 g dry tea leaves and 2.0 +/- 0.3 ng per 100 ml of tea drink). a corrinoid compound was partially purified and characterized from the tea leaves. the patterns of the purified compound by the silica gel 60 thin-layer chromatography and c18 reversed phased high-performance liquid chromatography were identical to those of authentic vitamin b(12). when 20 week old vitamin b(12) defici ...200414969549
effect of a 4-month tea intervention on oxidative dna damage among heavy smokers: role of glutathione s-transferase genotypes.glutathione s-transferase (gst), a member of the phase ii group of xenobiotic metabolizing enzymes, has been intensively studied at the levels of phenotype and genotype. the gst mu 1 (gstm1) and gst theta 1 (gstt1) genes have a null-allele variant in which the entire gene is absent. the null genotype for both enzymes has been associated with many different types of tumors. the aim of this study was to determine the possible differences in increased oxidative stress susceptibility to smoking with ...200414973088
caffeine synthase and related methyltransferases in plants.caffeine (1,3,7-trimethylxanthine) is a purine alkaloid present in high concentrations in tea and coffee and it is also found in a number of beverages such as coca cola. it is necessary to elucidate the caffeine biosynthetic pathway and to clone the genes related to the production of caffeine not only to determine the metabolism of the purine alkaloid but also to control the content of caffeine in tea and coffee. the available data support the operation of a xanthosine-->7-methylxanthosine-->7-m ...200414977590
role of hydrogen peroxide in bactericidal action of catechin.catechin (epicatechin (ec), epicatechin gallate (ecg), epigallocatechin (egc) and epigallocatechin gallate (egcg)), which occur in green tea and black tea, possess strong bactericidal action. we observed a reactive oxygen species that was generated from the catechins as the active mechanism: and this reactive oxygen was identified. egcg reacted with the dissolved oxygen in aqueous solution, resulting in the generation of hydrogen peroxide. hydrogen peroxide production derived from egcg rose with ...200414993788
a clinical study to assess the ability of a powered toothbrush to remove chlorhexidine/tea dental stain.a single-center, single-blind, two-way crossover study was performed to compare the effects of an electric powered toothbrush with a conventional manual toothbrush at removing chlorhexidine/tea tooth staining.200415016033
inhibitory effects of green tea polyphenols on the production of a virulence factor of the periodontal-disease-causing anaerobic bacterium porphyromonas gingivalis.the effect of polyphenolic compounds isolated from green tea (camellia sinensis) on the production of toxic end metabolites of porphyromonas gingivalis was investigated. green tea polyphenols completely inhibited the production of n-butyric acid and propionic acid at a concentration of 1.0-2.0 mg/ml in general anaerobic medium (gam). (-)-epigallocatechin gallate (egcg), which is a major component of tea polyphenols also inhibited the production of phenylacetic acid at 0.5 mg/ml in gam broth. in ...200415030231
short-term black tea intake modulates the excretion of urinary mutagens in rats treated with 2-amino-3-methylimidazo-[4,5-f]quinoline (iq): role of cyp1a2 upregulation.rats were exposed to black tea (2.5% w/v) as their sole drinking liquid for either 1 day or 1 month, while controls were maintained on water. after this treatment period, all animals received a single oral dose iq (2-amino-3-methylimidazo-[4,5-f]quinoline), and urine was collected for 48 h. mutagenic activity of the urine was determined in the ames test in the presence and absence of an activation system. the excretion of direct-acting mutagens was markedly reduced following tea intake, and was ...200415034640
ex vivo modulation of chemical-induced mutagenesis by subcellular liver fractions of rats treated with rooibos (aspalathus linearis) tea, honeybush (cyclopia intermedia) tea, as well as green and black (camellia sinensis) teas.male fischer rats were given unprocessed (not oxidized) and processed (oxidized) rooibos and honeybush teas as well as green and black teas as a sole source of drinking fluid for 10 weeks, and sub cellular liver fractions were prepared. cytosolic fractions of rats consuming the unprocessed herbal teas, green and black teas significantly (p < 0.05) protected against 2-acetylaminofluorene (2-aaf)-induced mutagenesis in the salmonella mutagenicity test with strain ta 98, using aroclor 1254-induced ...200415036128
[determination of low-level pesticide residues in agricultural products by ion-trap gc/ms/ms].the objective of this study was to elucidate the utility of ion-trap gc/ms/ms for the analysis of pesticides in extracted matrices from various agricultural products. identification and quantitative analysis of pesticides in matrices were performed by quadrupole gc/ms and ion-trap gc/ms/ms. chlorpyrifos was added to the matrix of spinach, soybean in the pod or corn, and aldrin, dieldrin, endrin, alpha-bhc, beta-bhc, gamma-bhc, delta-bhc, p,p'-ddd, p,p'-dde, o,p'-ddt and p,p'-ddt were added to ea ...200315038113
reaction of iron(iii) with theaflavin: complexation and oxidative products.theaflavins are a family of compounds, whose chemistry has been sparsely investigated. they can comprise up to 40% the dry weight of black tea. they are known to chelate metals, however very little knowledge exists on the mechanisms involved. there is some correlation between both of these areas in that following degradation of the iron theaflavin complex, subsequent redox reactions may lead to the formation of similar products on both occasions. the interaction of iron(iii) with theaflavin at p ...200415041246
tea polyphenols inhibit acetyl-coa:1-alkyl-sn-glycero-3-phosphocholine acetyltransferase (a key enzyme in platelet-activating factor biosynthesis) and platelet-activating factor-induced platelet aggregation.tea extracts have antiallergic and anti-inflammatory actions in rats and mice. however the mechanism through which tea polyphenols act in vivo are still incompletely understood. we found inhibitory effects of black tea extracts on an fmlp-induced aggregating response in a rabbit platelet-polymorphonuclear leukocyte (pmn) system.200415051936
determination of ni (ii) in beverages without any sample pretreatment by adsorptive stripping chronopotentiometry (adscp).the purpose of this paper was to use adsorptive stripping chronopotentiometry for the determination of ni (ii) in worldwide consumed beverages without any sample pretreatment, using dimethilglyoxime (dmg) as complexing agent and a glassy carbon mercury film electrode as the working electrode. ni (dmg)2 complex is adsorbed onto the mercury film at an electrolysis potential of -500 mv for 60 s and then reduced by a -5 microa constant cathodic current. the sensitivity of the method was studied for ...200415053517
development of methods to enhance extrinsic tooth discoloration for comparison of toothpastes. 2. two-product clinical study.extrinsic staining of teeth is considered to be unsightly and a number of 'whitening' toothpastes have been formulated to inhibit or remove such tooth discoloration. the aim of this study was to compare the stain prevention of two toothpastes.200415058367
dietary habits and cancer mortality among middle aged and older japanese living in hokkaido, japan by cancer site and sex.dietary factors are thought to be closely associated with the development of human cancers and hence numerous studies in this area have already been conducted in the united states and other western countries. comparatively few prospective studies have been published in japan, especially for hokkaido people. the present investigation was therefore performed to assess links between four leading cancers and some of the japanese common dietary factors through a cohort study (1984-2002) in hokkaido b ...200415075007
screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, dpph radical scavenging assay, and folin-ciocalteu assay.oxidation of low-density lipoprotein (ldl) has been implicated in atherogenesis. antioxidants that prevent ldl from oxidizing may reduce atherosclerosis. this study investigated ldl antioxidant activity in edible plant products for development of dietary supplementation to prevent atherosclerosis. fifty-two kinds of edible plants were extracted using 70% aqueous ethanol solution, and the antioxidant activity of the extracts, which inhibit human ldl oxidation induced by copper ion, was determined ...200415080652
effects of sweet and bitter gustatory stimuli in anorexia nervosa on eeg frequency spectra.the possible differences in processing gustatory stimuli in anorexic patients compared to healthy control subjects was investigated by electrophysiological methods. the electroencephalogram (eeg) was recorded in outpatients treated with anorexia nervosa (an) and age-matched controls after exposure to sweet (milk chocolate) and bitter (black tea) taste stimuli. power spectrum analysis was performed on eeg epochs recorded in the above conditions. compared to controls a significantly higher percent ...200415094251
strong inhibitory effects of common tea catechins and bioflavonoids on the o-methylation of catechol estrogens catalyzed by human liver cytosolic catechol-o-methyltransferase.in the present investigation, we studied the inhibitory effects of three tea catechins [catechin, epicatechin, and (-)-epigallocatechin-3-o-gallate] and two bioflavonoids (quercetin and fisetin) on the o-methylation of 2- and 4-hydroxyestradiol (2-oh-e(2) and 4-oh-e(2), respectively) by human liver cytosolic catechol-o-methyltransferase (comt). we found that catechin and epicatechin each inhibited the o-methylation of 2-oh-e(2) and 4-oh-e(2) in a concentration-dependent manner. the ic(50) values ...200415100171
modulation of tea and tea polyphenols on benzo(a)pyrene-induced dna damage in chang liver cells.the protective effects of three tea extracts (green tea, gte; oolong tea, ote; and black tea, bte) and five tea polyphenols (epicatechin, ec; epicatechin gallate, ecg; epigallocatechin, egc; epigallocatechin gallate, egcg; and theaflavins, thfs) on benzo[a]pyrene (b[a]p)-induced dna damage in chang liver cells were evaluated using the comet assay. b[a]p-induced dna damage in chang liver cells was significantly (p < 0.05) inhibited by gte and ote at a concentration of 10 microg/ml and by bte at 2 ...200415104213
analysis of theaflavins and thearubigins from black tea extract by maldi-tof mass spectrometry.black tea contains two major groups of pigments, theaflavins (tfs) and thearubigins (trs). tfs contain a bis-flavan substituted 1,2-dihydroxy-3,4-benzotropolone moiety. unlike the tfs, trs have not yet been characterized. the chemical structure of the trs remains a mystery. the present paper reports our effort to study the structure of tfs and trs using delayed pulsed ion extraction of ions generated via the matrix-assisted laser desorption ionization (maldi) technique, on line with a linear tim ...200415113141
pigments in green tea leaves (camellia sinensis) suppress transformation of the aryl hydrocarbon receptor induced by dioxin.environmental contaminants such as dioxins enter the body mainly through diet and cause various toxicities through transformation of the aryl hydrocarbon receptor (ahr). we previously reported that certain natural flavonoids at the dietary level suppress the ahr transformation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd). in this study, we identified lutein and chlorophyll a and b from green tea leaves as the novel antagonists for ahr. these active compounds suppressed ahr transformatio ...200415113147
modulation of beta-lactam resistance in staphylococcus aureus by catechins and gallates.aqueous extracts of japanese green tea (camellia sinensis) are able to reverse beta-lactam resistance in methicillin-resistant staphylococcus aureus (mrsa). we have attributed the capacity to reverse oxacillin resistance in the homogeneous pbp2a producer bb568 and in emrsa-16 to (-)-epicatechin gallate (ecg) and (-)-catechin gallate (cg). minimum inhibitory concentration (mic) values for oxacillin were reduced from 256 and 512 to 1-4 mg/l, respectively, in the presence of these polyphenols. in a ...200415120724
antioxidants of the beverage tea in promotion of human health.tea that contains many antioxidants is a pleasant and safe drink that is enjoyed by people across the globe. tea leaves are manufactured as black, green, or oolong. black tea represents approximately 78% of total consumed tea in the world, whereas green tea accounts for approximately 20% of tea consumed. the concept of "use of tea for promotion of human health and prevention and cure of diseases" has become a subject of intense research in the last decade. diseases for which tea drinkers appear ...200415130283
hplc-msn analysis of phenolic compounds and purine alkaloids in green and black tea.tea is a complex mixture containing a range of compounds from simple phenolics to complex thearubigins, many of which have well-recognized antioxidant properties. this paper describes the application of high-performance liquid chromatography-mass spectrometry (hplc-ms(n)) methods for the rapid and routine analysis of more than 30 phenolics in tea. green and black tea infusions were injected directly onto a reversed phase hplc column, and the phenolics eluted using two different mobile phase grad ...200415137818
inhibitory effect of some herbal extracts on adherence of streptococcus mutans.the objective of this study was to investigate the inhibitory effect of the crude extracts from some herbs on adherence of streptococcus mutans (s. mutans) atcc 25175 and tpf-1 in vitro. six herbs, andrographis paniculata; cassia alata; chinese black tea (camellia sinensis); guava (psidium guajava); harrisonia perforata and streblus asper, were extracted with 50 or 95% ethanol and dried. herbal extracted solution at 0.5% concentration (w/v) was initially tested for bacterial adherence on glass s ...200415138013
new dibenzotropolone derivatives characterized from black tea using lc/ms/ms.theaflavins and thearubigins are major pigments in black tea, and it is generally accepted that they are produced by oxidation of flavan-3-ols (catechins) during tea fermentation. in the course of studies on the oxidation mechanism of tea polyphenols, especially the formation of thearubigins, a method combining the enzymatic synthesis and lc/esi-ms/ms analysis was developed to search for new higher molecular weight polymers from black tea. three new dibenzotropolones, theadibenzotropolone a, b, ...200415142559
[hepatotoxicity due to camelia sinensis]. 200415153351
flavonoids from argentine tagetes (asteraceae) with antimicrobial activity.the flavonoids, constituting one of the most numerous and widespread groups of natural plant constituents, are important to humans not only because they contribute to plant colors but also because many members are physiologically active. these low-molecular-weight substances, found in all vascular plants, are phenylbenzopyrones. over 4000 structures have been identified in plant sources, and they are categorized into several groups. primarily recognized as pigments responsible for the autumnal b ...200415156042
black tea protects immunocytes from tumor-induced apoptosis by changing bcl-2/bax ratio.it is known that cancer is associated with altered immune function. we demonstrated earlier that black tea inhibits tumor growth in a dose-dependent manner. here, we report that apoptosis was the cause of immunocyte death in ehrlich's ascites carcinoma (eac)-bearing mice and anti-tumor dose of black tea restored eac-induced immunosuppression by inhibiting apoptosis. a search for the molecular mechanism revealed that eac burden increased the expression of the pro-apoptotic proteins p53 and bax in ...200415159016
identification of the astringent taste compounds in black tea infusions by combining instrumental analysis and human bioresponse.application of taste dilution analyses on freshly prepared black tea infusions revealed neither the high molecular weight thearubigen-like polyphenols nor the catechins and theaflavins, but a series of 14 flavon-3-ol glycosides as the main contributors to the astringent taste perceived upon black tea consumption. among these glycosides, the apigenin-8-c-[alpha-l-rhamnopyranosyl-(1-->2)-o-beta-d-glucopyranoside] was identified for the first time in tea infusions. depending on the structure, the f ...200415161222
polyphenolic compounds, antioxidant capacity, and quinone reductase activity of an aqueous extract of ardisia compressa in comparison to mate (ilex paraguariensis) and green (camellia sinensis) teas.aqueous extracts of the leaves of ardisia compressa (ac) have been used in folk medicine to treat various liver disorders including liver cancer. the objective of this study was to partially characterize and determine the total polyphenol content, antioxidant capacity, and quinone reductase activity of a. compressa tea in comparison to mate (ilex paraguariensis, mt) and green (camellia sinensis,gt) teas. total polyphenol content, antioxidant capacity, and phase ii enzyme induction capacity were ...200415161234
green and black teas inhibit atherosclerosis by lipid, antioxidant, and fibrinolytic mechanisms.tea is the most widely consumed beverage in the world, second only to water. most laypersons and scientists believe that green tea is healthier than black tea due to the low incidence of heart disease and cancer in the orient. here, we report the first dose-response comparison of a green and black tea on normal hamsters after long-term supplementation and on a hamster model of atherosclerosis. both teas were equally effective in inhibiting atherosclerosis with the lower dose decreasing it 26-46% ...200415161246
black tea increases coronary flow velocity reserve in healthy male subjects.epidemiologic studies suggest that tea consumption decreases the risk for cardiovascular events. however, there has been no clinical report examining the effects of tea consumption on coronary circulation. the purpose of this study was to evaluate the effects of black tea on coronary flow velocity reserve (cfvr) using transthoracic doppler echocardiography (ttde). this was a double-blind crossover study of 10 healthy male volunteers conducted to compare the effects of black tea and caffeine on c ...200415165919
lung cancer risk among nonsmoking women in relation to diet and physical activity.to investigate the relationship between diet, physical activity, and the risk of lung cancer among female nonsmokers, and to compare it with female smokers in the same population, we conducted a case-control study. data collected by personal interviews from 419 cases and 1593 controls were analyzed using unconditional logistic regression. as expected, among 130 nonsmoking cases, adenocarcinoma was the predominant cell type (49.2%), followed by squamous cell (20.2%) and small cell cancers (10.5%) ...200415190423
heavy metals in black tea samples produced in turkey. 200415200002
ros-related enzyme expressions in endothelial cells regulated by tea polyphenols.elevation of reactive oxygen species (ros), especially the level of superoxide is a key event in many forms of cardiovascular diseases. to study the mechanism of tea polyphenols against cardiovascular diseases, we observed the expressions of ros-related enzymes in endothelial cells.200415202862
inhibition of lung carcinogenesis and effects on angiogenesis and apoptosis in a/j mice by oral administration of green tea.oral administration of tea (camellia sinensis) has been shown to inhibit the formation and growth of several tumor types in animal models. the present study investigated the effects of treatment with different concentrations of green tea on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk)-induced lung tumorigenesis in female a/j mice. two days after a single dose of nnk (100 mg/kg body weight, i.p.), the mice were given 0.1, 0.2, 0.4, and 0.6% green tea solution (1, 2, 4, and 6 g of tea soli ...200415203377
inhibitory effect(s) of polymeric black tea polyphenol fractions on the formation of [(3)h]-b(a)p-derived dna adducts.five polymeric black tea polyphenol fractions (pbp-1-5) were isolated from a popular brand of black tea. the effect of these pbps and epigallocatechin gallate (egcg), a major green tea polyphenol, was studied on the formation of [(3)h]-b(a)p-derived dna adducts in vitro, employing rat liver microsomes. pbp-1-3 inhibited microsome-catalyzed [(3)h]-b(a)p-derived dna adduct formation in vitro in a dose-dependent manner. this inhibition was further enhanced on preincubation of microsomes with each o ...200415212478
increased microbicidal activity of green tea (camellia sinensis) in combination with butylated hydroxyanisole.we have demonstrated that green tea (camellia sinensis) shows increased antimicrobial activity against bacteria and fungi when used in combination with butylated hydroxyanisole (bha). glycolic extract taken from green tea showed only limited activity against streptococcus mutans and no activity against candida albicans and certain strains of escherichia coli. bha, at non inhibitory concentrations, increased the microbicidal activity of green tea against 10(10) s. mutans (p<0.01), non-susceptible ...200415216944
evidence for a prospective anti-osteoporosis effect of black tea (camellia sinensis) extract in a bilaterally ovariectomized rat model.the purpose of this study was to examine whether whole aqueous black tea extract (bte) prevents bone loss induced by ovarian hormone deficiency. eighteen 95-100 days old female albino rats were randomly assigned to three treatment groups [sham -operated control (sham); bilaterally ovariectomized (ovx) and ovx + aqueous black tea extract (bte) ] and sacrificed after 28 days. all animals were fed a standard laboratory diet with free access to deionized water except on days of urinary parameter stu ...200415228990
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