Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
synergized antimicrobial activity of eugenol incorporated polyhydroxybutyrate films against food spoilage microorganisms in conjunction with pediocin.biopolymers and biopreservatives produced by microorganisms play an essential role in food technology. polyhydroxyalkanoates and bacteriocins produced by bacteria are promising components to safeguard the environment and for food preservation applications. polyhydroxybutyrate (phb)-based antimicrobial films were prepared incorporating eugenol, from 10 to 200 μg/g of phb. the films were evaluated for antimicrobial activity against foodborne pathogens, spoilage bacteria, and fungi such as staphylo ...201323666640
effects of nisin-incorporated films on the microbiological and physicochemical quality of minimally processed mangoes.the aim of this study is to examine the effects of nisin-incorporated cellulose films on the physicochemical and microbiological qualities of minimally processed mangoes. the use of antimicrobial films did not affect the physicochemical characteristics of mangoes and showed antimicrobial activity against staphylococcus aureus, listeria monocytogenes, alicyclobacillus acidoterrestris and bacillus cereus. the mango slices were inoculated with s. aureus and l. monocytogenes (10(7)cfu/g), and the vi ...201323673058
synthesis of poly-(3-hydroxybutyrate-co-12 mol % 3-hydroxyvalerate) by bacillus cereus fb11: its characterization and application as a drug carrier.the synthesis of microbial polyhydroxyalkanoate is investigated in this work for it potential application as drug carrier for cancer therapy. the bacterial isolate bacillus cereus fb11 has synthesized poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer under nutrient stress conditions using glucose as a sole carbon source. the ftir spectrum of the purified copolymer showed the characteristic absorption bands at 1,719, 1,260 and 2,931 cm(-1) attributing to c=o, c-o stretching and c-h vibratio ...201323674059
bacillus subtilis as a tool for screening soil metagenomic libraries for antimicrobial activities.finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign dna. therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. here we evaluated the use of the escherichia coli-bacillus subtilis shuttle vector pht01 to construct a forest-soil metagenomic library. this library was screened in both hosts for antimicrob ...201323676924
[transcriptional regulation of exosporium basal layer structural gene exsb in bacillus thuringiensis by sigmak and gere].to identify the transcriptional regulation of exosporium basal layer structural gene exsb in bacillus thuringiensis.201323678570
potential applications for annona squamosa leaf extract in the treatment and prevention of foodborne bacterial disease.foodborne disease is a major public health problem. the present study examined annona squamosa leaves, which are traditionally used to treat diarrhea and other infections, for their potential to be used in modern food safety or medicine. active constituents were partially purified by ethanol extraction and column chromatography. mics of the extract were 62.5 to 125 microg/ml against bacillus cereus, listeria monocytogenes and staphylococcus aureus, and 250 microg/ml against campylobacter jejuni. ...201323678817
causes of infectious acute diarrhea in infants treated at pediatric clinic.the aim of this work has been a presentation of causes of acute infectious diarrhea.201323678831
two rep genes in small cryptic plasmid pkst21 of escherichia coli.the complete nucleotide sequence of a small cryptic plasmid pkst21 from escherichia coli was determined. this plasmid is 1,460 bp long with an overall gc content of 51 %. based on sequence analysis, the presence of two segments with different average gc density was observed. the segment with higher gc content revealed 98-90 % similarity to several small plasmids of e. coli and to pcr1 from gram-positive corynebacterium renale. plasmid pkst21 possesses two conversely oriented open reading frames ...201323680975
sphingomyelin regulates the transbilayer movement of diacylglycerol in the plasma membrane of madin-darby canine kidney cells.diacylglycerol (dag) is a key component in lipid metabolism and signaling. previous model membrane studies using dag analogs suggest their rapid membrane transbilayer movement. however, little is known about the dag distribution and dynamics in cell membranes. using live-cell fluorescence microscopy, we monitored the transbilayer movement of dag with the yellow fluorescent protein-tagged c1ab domain from protein kinase c-γ (eyfp-c1ab), which selectively binds dag. when hela cells were treated wi ...201323682124
2,2'-dithienyl diselenide pro-oxidant activity accounts for antibacterial and antifungal activities.the aim of this study was to explore if 2,2'-dithienyl diselenide (dtds) pro-oxidant activity is related to its antibacterial and antifungal actions. the antimicrobial activity of dtds against bacterial and fungal was investigated in the broth microdilution assay (3.02-387 μg/ml). additionally, the survival curve of microorganisms in the presence of dtds (12.09-193.5 μg/ml) was performed. the involvement of pro-oxidant activity in the dtds antimicrobial action was investigated by supplementing t ...201323683588
antimicrobial activity of schinus lentiscifolius (anacardiaceae).schinus lentiscifolius marchand (syn. schinus weinmannifolius engl) is a plant native to rio grande do sul (southern brazil) and has been used in brazilian traditional medicine as antiseptic and antimicrobial for the treatment of many different health problems as well as to treat leucorrhea and to assist in ulcer and wound healing. although it is a plant widely used by the population, there are no studies proving this popular use.201323684720
biological activity, design, synthesis and structure activity relationship of some novel derivatives of curcumin containing sulfonamides.five series of curcumin derivatives with sulfonamides 3a-3e, 4a-4e, 5a-5e, 6a-6e and 7a-7e have been synthesized and evaluated for in vitro antibacterial activity against selected medically important gram-(+) and gram-(-) bacterial species viz. staphylococcus aureus, bacillus cereus, salmonella typhi, pseudomonas aeruginosa and escherichia coli, and antifungal activity against few pathogenic fungal species viz. aspergillus niger, aspergillus flavus, trichoderma viride and curvularia lunata. the ...201323685942
functional screening of metagenome and genome libraries for detection of novel flavonoid-modifying enzymes.the functional detection of novel enzymes other than hydrolases from metagenomes is limited since only a very few reliable screening procedures are available that allow the rapid screening of large clone libraries. for the discovery of flavonoid-modifying enzymes in genome and metagenome clone libraries, we have developed a new screening system based on high-performance thin-layer chromatography (hptlc). this metagenome extract thin-layer chromatography analysis (meta) allows the rapid detection ...201323686272
expression, crystallization and preliminary x-ray crystallographic analysis of aldehyde dehydrogenase (aldh) from bacillus cereus.aldehyde dehydrogenase (aldh) catalyses the oxidation of aldehydes using nad(p)(+) as a cofactor. most aldehydes are toxic at low levels. aldhs are used to regulate metabolic intermediate aldehydes. the aldh gene from bacillus cereus was cloned and the aldh protein was expressed, purified and crystallized. a crystal of the aldh protein diffracted to 2.6 å resolution and belonged to the monoclinic space group p21, with unit-cell parameters a = 83.5, b = 93.3, c = 145.5 å, β = 98.05°. four protome ...201323695569
identification of cultivable bacteria in the intestinal tract of bactrocera dorsalis from three different populations and determination of their attractive potential.this study aimed to identify the cultivable bacteria inhabiting the intestinal tract of adult oriental fruit flies (bactrocera dorsalis) from laboratory-reared, laboratory sterile sugar-reared, and field-collected populations, and to evaluate the attractiveness of the metabolites produced by the above bacteria to their hosts.201423696217
cooperation and the evolutionary ecology of bacterial virulence: the bacillus cereus group as a novel study system.how significant is social evolution theory for the maintenance of virulence in natural populations? we assume that secreted, distantly acting virulence factors are highly likely to be cooperative public goods. using this assumption, we discuss and critically assess the potential importance of social interactions for understanding the evolution, diversity and distribution of virulence in the bacillus cereus group, a novel study system for microbial social biology. we conclude that dynamic equilib ...201323702950
antibacterial effectiveness of chitosan-propolis coated polypropylene films against foodborne pathogens.antibacterial properties of chitosan are well documented in the literature. however its antibacterial effectiveness in the film form is controversial due to the methodological differences in test methods used. in this study, antibacterial effectiveness of chitosan-coated polypropylene films alone and incorporating ethanolic extract of propolis (eep) were evaluated against six foodborne pathogens (bacillus cereus, cronobacter sakazakii, escherichia coli o157:h7, listeria monocytogenes, salmonella ...201323707735
plant-based hydrocarbon esters from tragia involucrata possess antimicrobial and anti-inflammatory activities.antimicrobial and anti-inflammatory activities of hydrocarbon esters obtained from tragia involucrata were evaluated by disk-diffusion (250 µg/ml), and broth-dilution (500-7.8 µg/ml), methods against bacteria. among the compounds, shellsol showed the most potent activity against burkholderia pseudomallei (khw), aeromonas hydrophila, staphylococcus aureus, bacillus subtilis, streptococcus pyogenes, klebsiella pneumoniae, proteus mirabilis, and streptococcus pneumoniae. interestingly, vinyl hexyle ...201323713670
aerobic biodegradation of 2,4,6-trinitrotoluene (tnt) by bacillus cereus isolated from contaminated soil.in this study, biological degradation of 2,4,6-trinitrotoluene (tnt) which is very highly toxic environmentally and an explosive in nitroaromatic character was researched in minimal medium by bacillus cereus isolated from north atlantic treaty organization (nato) tnt-contaminated soils. in contrast to most previous studies, the capability of this bacteria to transform in liquid medium containing tnt was investigated. during degradation, treatment of tnt was followed by high-performance liquid ch ...201323715804
development of blood-yolk-polymyxin b-trimethoprim agar for the enumeration of bacillus cereus in various foods.blood-yolk-polymyxin b-trimethoprim agar (bypta) was developed by the addition of egg yolk, laked horse blood, sodium pyruvate, polymyxin b, and trimethoprim, and compared with mannitol-yolk-polymyxin b agar (mypa) for the isolation and enumeration of bacillus cereus (b. cereus) in pure culture and various food samples. in pure culture, there was no statistical difference (p>0.05) between the recoverability and sensitivity of mypa and bypta, whereas bypta exhibited higher specificity (p<0.05). t ...201323732830
differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from burkholderia cepacia and pseudomonas pseudoflava.the aim of this study is determine the relative sensitivity of a panel of seven polyhydroxyalkanoate producing bacteria to a panel of seven lignocellulosic-derived fermentation inhibitors representing aliphatic acids, furans and phenolics. a further aim was to measure the polyhydroxybutyrate production of select organisms on lignocellulosic-derived monosaccharides arabinose, xylose, glucose and mannose.201323734728
methyl carnosate, an antibacterial diterpene isolated from salvia officinalis leaves.ethanolic extracts of salvia officinalis leaves demonstrated antibacterial activity against bacillus cereus. fractionation of the extracts led to the isolation of the most active antibacterial compound, which, from spectroscopic and lc-ms evidence, was proved to be the diterpene, methyl carnosate.201323738442
biodegradation of benzo[a]pyrene by the mixed culture of bacillus cereus and bacillus vireti isolated from the petrochemical industry.polycyclic aromatic hydrocarbons are a group of compounds that pose threat to humans and animal life. methods to reduce the amount of pahs in the environment are continuously being sought. the bacterial consortium capable of utilizing benzo(a)pyrene as the sole source of carbon and energy was isolated from petrochemical soil. the isolates were identified as bacillus cereus and bacillus viretibased on morphological characterization, and 16s rdna gene sequence analysis. about 58.98% of benzo(a)pyr ...201223741789
rapid identification of dairy mesophilic and thermophilic sporeforming bacteria using dna high resolution melt analysis of variable 16s rdna regions.due to their ubiquity in the environment and ability to survive heating processes, sporeforming bacteria are commonly found in foods. this can lead to product spoilage if spores are present in sufficient numbers and where storage conditions favour spore germination and growth. a rapid method to identify the major aerobic sporeforming groups in dairy products, including bacillus licheniformis group, bacillus subtilis group, bacillus pumilus group, bacillus megaterium, bacillus cereus group, geoba ...201323743474
combinational expression of sorbose/sorbosone dehydrogenases and cofactor pyrroloquinoline quinone increases 2-keto-l-gulonic acid production in ketogulonigenium vulgare-bacillus cereus consortium.the expression levels of sorbose/sorbosone dehydrogenase genes (sdh and sndh) and the synthesis genes (pqqabcden) of the adjoint cofactor pyrroloquinoline quinone (pqq) were genetically manipulated in ketogulonigenium vulgare to increase the production of 2-keto-l-gulonic acid (2-klg), the precursor of vitamin c, in the consortium of k. vulgare and bacillus cereus. we found that overexpression of sdh-sndh alone in k. vulgare could not significantly enhance the production of 2-klg, revealing the ...201323747604
synthesis and antibacterial activities of novel nanocomposite films of chitosan/phosphoramide/fe3o4 nps.new nanocomposite films of chitosan/phosphoramide/1-5% fe3o4 nps were prepared. the fe3o4 nps were synthesized as core-shell nps with starch as a capping agent. the phosphoramide compound was synthesized from the reaction of morpholine with n-4-nitrophenyl phosphoramidic dichloride. the xrd and sem analysis of fe3o4 nps, chitosan (cs), phosphoramide (ph), cs/ph and cs/fe3o4 nps films as well as the nanocomposite films 1-5 containing cs/ph/1-5% fe3o4 nps were investigated and the results confirme ...201323748004
the pore-forming haemolysins of bacillus cereus: a review.the bacillus cereus sensu lato group contains diverse gram-positive spore-forming bacteria that can cause gastrointestinal diseases and severe eye infections in humans. they have also been incriminated in a multitude of other severe, and frequently fatal, clinical infections, such as osteomyelitis, septicaemia, pneumonia, liver abscess and meningitis, particularly in immuno-compromised patients and preterm neonates. the pathogenic properties of this organism are mediated by the synergistic effec ...201323748204
a novel combinatorial biocatalytic approach for producing antibacterial compounds effective against mycobacterium tuberculosis (tb).two bacterial hosts expressing cloned aromatic oxygenases were used to catalyze the oxidation and polymerization of indole and related substrates, creating mixtures of indigoid compounds comprised of novel dimers and trimers. crude extracts and purified compounds were tested for their ability to inhibit the growth of gram-positive organisms, in general, and mycobacterium tuberculosis (tb), in particular. of the 74 compounds tested against m. tuberculosis, ~66 % had minimum inhibitory concentrati ...201323749163
bioactive phenylalanine derivatives and cytochalasins from the soft coral-derived fungus, aspergillus elegans.one new phenylalanine derivative 4'-ome-asperphenamate (1), along with one known phenylalanine derivative (2) and two new cytochalasins, aspochalasin a1 (3) and cytochalasin z24 (4), as well as eight known cytochalasin analogues (5-12) were isolated from the fermentation broth of aspergillus elegans zj-2008010, a fungus obtained from a soft coral sarcophyton sp. collected from the south china sea. their structures and the relative configurations were elucidated using comprehensive spectroscopic ...201323752358
cross-functionalities of bacillus deacetylases involved in bacillithiol biosynthesis and bacillithiol-s-conjugate detoxification pathways.bshb, a key enzyme in bacillithiol biosynthesis, hydrolyses the acetyl group from n-acetylglucosamine malate to generate glucosamine malate. in bacillus anthracis, ba1557 has been identified as the n-acetylglucosamine malate deacetylase (bshb); however, a high content of bacillithiol (~70%) was still observed in the b. anthracis ∆ba1557 strain. genomic analysis led to the proposal that another deacetylase could exhibit cross-functionality in bacillithiol biosynthesis. in the present study, ba155 ...201323758290
physiological and transcriptional response of bacillus cereus treated with low-temperature nitrogen gas plasma.this study was conducted to investigate the inactivation kinetics of bacillus cereus vegetative cells upon exposure to low-temperature nitrogen gas plasma and to reveal the mode of inactivation by transcriptome profiling.201323758316
antibacterial activity of selected malaysian honey.antibacterial activity of honey is mainly dependent on a combination of its peroxide activity and non-peroxide components. this study aims to investigate antibacterial activity of five varieties of malaysian honey (three monofloral; acacia, gelam and pineapple, and two polyfloral; kelulut and tualang) against staphylococcus aureus, bacillus cereus, escherichia coli, and pseudomonas aeruginosa.201323758747
biological and chemical detection of fumonisins produced on agar medium by fusarium verticillioides isolates collected from corn in sohag, egypt.fusarium verticillioides (sacc.) nirenberg is among the most common fusarium species corn pathogens worldwide, and has been recognized as a fumonisin b1 (fb1) and fumonisin b2 (fb2) producer. in the present work, extracts of 58 f. verticillioides isolates from corn samples collected from sohag governorate, egypt, were tested for their biotoxicity and production of fumonisin toxins. forty-four fusarium verticillioides isolates out of 58 tested produced fb1 or fb1 and fb2 (15 and 29 isolates, resp ...201323760819
microbiological quality of chicken- and pork-based street-vended foods from taichung, taiwan, and laguna, philippines.the microbiological quality of chicken- and pork-based street-food samples from taichung, taiwan's night markets (50) and laguna, philippines' public places (69) was evaluated in comparison to a microbiological guideline for ready-to-eat foods. different bacterial contamination patterns were observed between 'hot-grilled' and 'cold cooked/fried' food types from the two sampling locations with 'hot grilled' foods generally showing better microbiological quality. several samples were found to be u ...201323764220
family portrait of bacillus cereus and bacillus weihenstephanensis cereulide-producing strains.two thousand bacillus cereus sensu lato isolates from food and environmental matrices were screened by pcr for the presence of cereulide-producing strains. this survey identified 73 potential emetic strains, most of which originated from non-random food and clinical samplings. none of the 460 bacillus thuringiensis, bacillus mycoides and bacillus pseudomycoides strains were pcr-positive for the cereulide genetic determinants. the chromosomal and extrachromosomal gene pool diversity of a subset o ...200923765791
razor ex anthrax air detection system for detection of bacillus anthracis spores from aerosol collection samples: collaborative study.the razor ex anthrax air detection system was validated in a collaborative study for the detection of bacillus anthracis in aerosol collection buffer. phosphate-buffered saline was charged with 1 mg/ml standardized dust to simulate an authentic aerosol collection sample. the dust-charged buffer was spiked with either b. anthracis ames at 2000 spores/ml or bacillus cereus at 20 000 spores/ml. twelve collaborators participated in the study, with four collaborators at each of three sites. each coll ...201323767365
bacillus cereus and severe intestinal infections in preterm neonates: putative role of pooled breast milk.bacillus cereus is an environmental pathogen whose spores resist the usual cleaning procedure applied by the food industry and hospitals. we reported a cluster of severe intestinal infections due to b cereus in 2 very low birth weight neonates from a neonatal intensive care unit.201323769834
nucleation of platelets with blood-borne pathogens on kupffer cells precedes other innate immunity and contributes to bacterial clearance.through the use of intravital imaging of the liver, we demonstrate a collaborative role for platelets with kupffer cells (kcs) in eradicating blood-borne bacterial infection. under basal conditions, platelets, via the platelet-adhesion receptor gpib, formed transient 'touch-and-go' interactions with von willebrand factor (vwf) constitutively expressed on kcs. bacteria such as bacillus cereus and methicillin-resistant staphylococcus aureus (mrsa) were rapidly caught by kcs and triggered platelets ...201323770641
structural and biochemical characterization of an active arylamine n-acetyltransferase possessing a non-canonical cys-his-glu catalytic triad.arylamine n-acetyltransferases (nats), a class of xenobiotic-metabolizing enzymes, catalyze the acetylation of aromatic amine compounds through a strictly conserved cys-his-asp catalytic triad. each residue is essential for catalysis in both prokaryotic and eukaryotic nats. indeed, in (human)nat2 variants, mutation of the asp residue to asn, gln, or glu dramatically impairs enzyme activity. however, a putative atypical nat harboring a catalytic triad glu residue was recently identified in bacill ...201323770703
bacillus cereus in infant foods: prevalence study and distribution of enterotoxigenic virulence factors in isfahan province, iran.this study was carried out in order to investigate the presences of bacillus cereus and its enterotoxigenic genes in infant foods in isfahan, iran. overall 200 infant foods with various based were collected and immediately transferred to the laboratory. all samples were culture and the genomic dna was extracted from colonies with typical characters of bacillus cereus. the presences of enterotoxigenic genes were investigated using the pcr technique. eighty-four of two hundred samples (42%) were f ...201323781153
skin-associated bacillus, staphylococcal and micrococcal species from the house dust mite, dermatophagoides pteronyssinus and bacteriolytic enzymes.dust mites produce bacteriolytic enzymes, one of which belongs to the nlpc/p60 superfamily comprising bacterial and fungal proteins. whether this enzyme is derived from the mite or from mite-associated microbes is unclear. to this end, the bacteriology of mites per se, and carpet and mattress dust from a group of asthmatic children and their parents was investigated. dust from parents' and children's mattresses yielded significantly more colony forming units compared with dust from their corresp ...201323783892
microbial toxicity of ethanolamines--multiwalled carbon nanotubes.in the present study, antimicrobial activities of multiwalled carbon nanotubes (mwcnts) functionalized with ethanolamine (ea) groups were investigated. therefore, mwcnt were first functionalized with mono-, di-, and triethanolamine (mea, dea, and tea) under microwave technique. development of functional groups on the mwcnt surface was confirmed by fourier transform infrared and thermogravimetric analysis. morphological variation was investigated by transmission electron microscopy. then, antimic ...201423784887
a novel synthetic lipopeptide is allergy-protective by the induction of lps-tolerance.exposure to the environment of traditional farms can protect children from some allergic disease. due to this exposure, tlr2 expression in these children is increased. tlr2 ligands derived from gram-positive bacteria are found in the dust of these farms.201323786285
spectroscopic and solvatochromism studies along with antioxidant and antibacterial activities investigation of 2-((2-mercaptophenylimino)methyl)-4-(phenyldiazenyl)phenol.the condensation reaction of 2-hydroxy-5-(aryldiazenyl)benzaldehyde (1a-d) with 2-aminothiophenole affored schiff base compounds 2-((2-mercaptophenylimino)methyl-4-(-aryldiazenyl)phenol (2a-d). the structures of compounds (2a-d) were characterized by elemental analysis, mass, infrared, uv-vis and nmr spectroscopy. the 2a-d shows enol-keto tautomeric and positive solvatochromism. the antibacterial activities of 2a-d were also evaluated by disc diffusion method. compound 2b displayed activity agai ...201323786981
synthesis and biological evaluation of some novel thiazole compounds as potential anti-inflammatory agents.in the present investigation, furo[2,3-d]thiazol-5(2h)-one 5 was obtained from reaction of thiosemicarbazone derivative 2 with diethyl acetylene dicarboxylate. a series of newly synthesized 2-(hydrazinyl)thiazol-4(5h)-one 6, 7 &8 and 2-(4-(substituted)-thiazol-2-yl)hydrazono derivatives 9a, b &10 were synthesized from treatment of thiosemicarbazone derivative 2 with appropriate α-halogenated compounds. also, a one pot synthesis of thiazole derivatives 13 &15 was achieved from three components re ...201323787438
growth-inhibitory activity of natural and synthetic isothiocyanates against representative human microbial pathogens.the aim of this study was to test the growth inhibition activity of isothiocyanates (itcs), defence compounds of plants, against common human microbial pathogens.201323789822
anti-biofilm forming and anti-quorum sensing activity of selected essential oils and their main components on food-related micro-organisms.the aim of this study was to investigate the effect of clary sage, juniper, lemon and marjoram essential oils (eos) and their major components on the formation of bacterial and yeast biofilms and on the inhibition of ahl-mediated quorum sensing (qs).201323789847
antibacterial and antimutagenic activities of novel zerumbone analogues.zerumbone, the key constituent of zingiber zerumbet smith, is a very important bioactive phytochemical. two new compounds viz. azazerumbone 1 and azazerumbone 2 were synthesised by zncl2-catalysed beckmann rearrangement of the zerumbone oxime. the structure elucidation of these analogues of zerumbone was carried out by 1d ((1)h nmr and (13)c nmr) and 2d-nmr (cosy, hsqc and noesy) spectral analysis. studies on the antibacterial activity established that azazerumbone 2 had better activity than zer ...201323790891
description of bacillus toyonensis sp. nov., a novel species of the bacillus cereus group, and pairwise genome comparisons of the species of the group by means of ani calculations.strain bct-7112(t) was isolated in 1966 in japan from a survey designed to obtain naturally occurring microorganisms as pure cultures in the laboratory for use as probiotics in animal nutrition. this strain, which was primarily identified as bacillus cereus var toyoi, has been in use for more than 30 years as the active ingredient of the preparation toyocerin(®), an additive for use in animal nutrition (e.g. swine, poultry, cattle, rabbits and aquaculture). despite the fact that the strain was i ...201323791203
peptide-binding dependent conformational changes regulate the transcriptional activity of the quorum-sensor nprr.the transcriptional regulator nprr controls the expression of genes essential for the adaptative response of bacillus cereus. nprr belongs to the rnpp family of directly regulated quorum sensors from gram-positive bacteria. it is activated by the re-imported signaling peptide nprx. to elucidate the activation mechanism of this quorum-sensing system, we analyzed the conformation changes induced on binding of nprx. we solved the crystal structure of the nprr/nprx binary complex and characterized t ...201323793817
development of a time-to-detect growth model for heat-treated bacillus cereus spores.the microbiological safety and quality of refrigerated processed foods of extended durability (repfeds) relies on a combination of mild heat treatment and refrigeration, sometimes in combination with other inhibitory agents that are ineffective when used alone. in this context, a predictive model describing the time-to-detect growth (measured by turbidimetry) of psychrotrophic bacillus cereus spores submitted to various combinations of ph, water activity (aw), heat treatment and storage temperat ...201323796655
[isolation and purification of recombinant human lactoferrin (rhlf) from transgenic rice and its antibacterial activities].to study the isolation and purification of recombinant human lactoferrin (rhlf) from transgenic rice, and to check its antibacterial activities.201323805514
extractive bioconversion of cyclodextrins by bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system.an extractive bioconversion with bacillus cereus cyclodextrin glycosyltransferase (cgtase, ec 2.4.1.19) in aqueous two-phase system (atps) was investigated for the synthesis and recovery of cyclodextrins (cds). optimum condition for the extractive bioconversion of cds was achieved in atps consisted of 7.7% (w/w) polyethylene glycol (peg) 20,000 and 10.3% (w/w) dextran t500 with volume ratio (vr) of 4.0. enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the produ ...201323806510
polyphosphate storage during sporulation in the gram-negative bacterium acetonema longum.using electron cryotomography, we show that the gram-negative sporulating bacterium acetonema longum synthesizes high-density storage granules at the leading edges of engulfing membranes. the granules appear in the prespore and increase in size and number as engulfment proceeds. typically, a cluster of 8 to 12 storage granules closely associates with the inner spore membrane and ultimately accounts for ∼7% of the total volume in mature spores. energy-dispersive x-ray spectroscopy (edx) analyses ...201323813732
direct analysis of water content and movement in single dormant bacterial spores using confocal raman microspectroscopy and raman imaging.heavy water (d2o) has a distinct molecular vibration spectrum, and this has been used to analyze the water content, distribution, and movement in single dormant bacillus cereus spores using confocal raman microspectroscopy and raman imaging. these methods have been used to measure the kinetics of d2o release from spores suspended in h2o, the spatial distribution of d2o in spores, and the kinetics of d2o release from spores during dehydration in air at room temperature. the results obtained were ...201323815288
transcriptional and proteomic stress responses of a soil bacterium bacillus cereus to nanosized zero-valent iron (nzvi) particles.nanosized zero valent iron (nzvi) is emerging as an option for treating contaminated soil and groundwater even though the potentially toxic impact exerted by nzvi on soil microorganisms remains uncertain. in this work, we focus on nanotoxicological studies performed in vitro using commercial nzvi and one common soil bacterium (bacillus cereus). results showed a negative impact of nzvi on b. cereus growth capability, consistent with the entrance of cells in an early sporulation stage, observed by ...201323816452
evolutionary dynamics of the accessory genome of listeria monocytogenes.listeria monocytogenes, a foodborne bacterial pathogen, is comprised of four phylogenetic lineages that vary with regard to their serotypes and distribution among sources. in order to characterize lineage-specific genomic diversity within l. monocytogenes, we sequenced the genomes of eight strains from several lineages and serotypes, and characterized the accessory genome, which was hypothesized to contribute to phenotypic differences across lineages. the eight l. monocytogenes genomes sequenced ...201323825666
bacillus cereus immune escape: a journey within macrophages.during bacterial infection, professional phagocytes are attracted to the site of infection, where they constitute a first line of host cell defense. their function is to engulf and destroy the pathogens. thus, bacteria must withstand the bactericidal activity of professional phagocytes, including macrophages to counteract the host immune system. bacillus cereus infections are characterized by bacteremia despite the accumulation of inflammatory cells at the site of infection. this implies that th ...201323827020
antibacterial activity of actinomycetes isolated from different soil samples of sheopur (a city of central india).the main objective of the present study was isolation, purification, and characterization of actinomycetes from soil samples, having antimicrobial activity against 12 selected pathogenic strains. soils samples were taken from different niche habitats of sheopur district, madhya pradesh, india. these samples were serially diluted and plated on actinomycete isolation agar media. potential colonies were screened, purified, and stored in glycerol stock. isolates were morphologically and biochemicall ...201323833752
complete ¹h, ¹⁵n, and ¹³c resonance assignments of bacillus cereus metallo-β-lactamase and its complex with the inhibitor r-thiomandelic acid.β-lactamases inactivate β-lactam antibiotics by hydrolysis of their endocyclic β-lactam bond and are a major cause of antibiotic resistance in pathogenic bacteria. the zinc dependent metallo-β-lactamase enzymes are of particular concern since they are located on highly transmissible plasmids and have a broad spectrum of activity against almost all β-lactam antibiotics. we present here essentially complete (>96%) backbone and sidechain sequence-specific nmr resonance assignments for the bacillus ...201423838816
characterization and application of enterocin rm6, a bacteriocin from enterococcus faecalis.use of bacteriocins in food preservation has received great attention in recent years. the goal of this study is to characterize enterocin rm6 from enterococcus faecalis osy-rm6 and investigate its efficacy against listeria monocytogenes in cottage cheese. enterocin rm6 was purified from e. faecalis culture supernatant using ion exchange column, multiple c18-silica cartridges, followed by reverse-phase high-performance liquid chromatography. the molecular weight of enterocin rm6 is 7145.0823 as ...201323844357
short communication: presence of neutral metallopeptidase (npr) gene and proteolytic activity of bacillus cereus isolated from dairy products.the control of proteolytic microorganisms is one of the main challenges of the dairy industry, due to their spoilage activity that jeopardizes the quality of their products. seventy-four bacillus cereus strains isolated from powdered, uht, and pasteurized milks were tested for the presence of the neutral metallopeptidase (npr) gene and proteolytic activity at 7, 10, 25, 30, and 37°c. all strains had the npr gene, and proteolytic activity increased with the incubation temperature. the obtained re ...201323849645
purification and characterization of two polyhydroxyalcanoates from bacillus cereus.this work aimed to study the potential of 155 strains of bacillus sp., isolated from a collection of tunisian microorganisms, for polyhydroxyalcanoates production. the strains were submitted to a battery of standard tests commonly used for determining bioplastic properties. the findings revealed that two of the isolates, namely bacillus us 163 and us 177, provided red excitations at a wavelength of approximately 543 nm. the polyhydroxyalcanoates produced by the two strains were purified. gas chr ...201323850680
live-cell imaging tool optimization to study gene expression levels and dynamics in single cells of bacillus cereus.single-cell methods are a powerful application in microbial research to study the molecular mechanism underlying phenotypic heterogeneity and cell-to-cell variability. here, we describe the optimization and application of single-cell time-lapse fluorescence microscopy for the food spoilage bacterium bacillus cereus specifically. this technique is useful to study cellular development and adaptation, gene expression, protein localization, protein mobility, and cell-to-cell communication over time ...201323851094
comparative studies to assess bacterial communities on the clover phylloplane using mlst, dgge and t-rflp.denaturing gradient gel electrophoresis (dgge) and terminal restriction fragment length polymorphism (t-rflp) were used to characterise the changes that occurred in bacillus cereus group strains present in the phylloplane of clover trifolium hybridum over 4 months. these strains had previously been analysed by multiple locus sequence typing (mlst). dgge displayed many equally intense bands which indicated many equally abundant ribotypes. the bacterial community composition was variable and the l ...201423861044
effects of lipid emulsion and multivitamins on the growth of microorganisms in peripheral parenteral nutrition solutions.blood stream infections caused by bacillus cereus or serratia marcescens in patients receiving peripheral parenteral nutrition (ppn) have occasionally been reported in japan, but these microorganisms are not major causes of blood stream infections in patients receiving total parenteral nutrition via a central venous catheter. in japan, commercially available ppn solutions contain amino acids, glucose, and electrolytes, but not contain lipid emulsion (le) and multivitamins (mv). in this study, th ...201323869182
bioactive metabolites from an endophytic cryptosporiopsis sp. inhabiting clidemia hirta.an endophytic cryptosporiopsis sp. was isolated from clidemia hirta and analyzed for its secondary metabolites that lead to the isolation of three bioactive molecules. the compounds were purified from the culture broth of the fungus and their structures were determined by spectroscopic methods as (r)-5-hydroxy-2-methylchroman-4-one (1), 1-(2,6-dihydroxyphenyl)pentan-1-one (2) and (z)-1-(2-(2-butyryl-3-hydroxyphenoxy)-6-hydroxyphenyl)-3-hydroxybut-2-en-1-one (3). compound 1 exhibited significant ...201323870821
antimicrobial activity of amazon astrocaryum aculeatum extracts and its association to oxidative metabolism.several compounds present in fruits as polyphenols are able to kill or inhibit the growth of microorganisms. these proprieties are relevant mainly in tropical areas, as amazonian region where infectious are highly prevalent. therefore, this study investigated the antimicrobial activity of tucumã amazonian fruit against 37 microorganisms. the potential role of oxidative metabolism imbalance was also studied as causal mechanism of antimicrobial activity. the results showed antibacterial effect of ...201423870852
characterization of amiba2446, a novel bacteriolytic enzyme active against bacillus species.there continues to be a need for developing efficient and environmentally friendly treatments for bacillus anthracis, the causative agent of anthrax. one emerging approach for inactivation of vegetative b. anthracis is the use of bacteriophage endolysins or lytic enzymes encoded by bacterial genomes (autolysins) with highly evolved specificity toward bacterium-specific peptidoglycan cell walls. in this work, we performed in silico analysis of the genome of bacillus anthracis strain ames, using a ...201323872558
biochemical characterization and antitumor study of l-glutaminase from bacillus cereus mtcc 1305.l-glutaminase (e.c.3.5.2.1) extracellularly produced by bacillus cereus mtcc 1305 was purified to apparent homogeneity with a fine band. the molecular weight of native enzyme and its subunit were found to be approximately 140 and 35 kda, respectively, which indicates its homotetrameric nature. the substrate specificity test of this enzyme showed its specificity for l-glutamine. the purified enzyme showed maximum activity at optimum ph 7.5 and temperature 35 °c. the enzyme retained stability up t ...201323873638
community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees.cellulolytic bacteria in forest soil provide carbon sources to improve the soil fertility and sustain the nutrient balance of the forest ecological system through the decomposition of cellulosic remains. these bacteria can also be utilized for the biological conversion of biomass into renewable biofuels. in this study, the community compositions and activities of cellulolytic bacteria in the soils of forests planted with broad-leaved deciduous (chang qing garden, cqg) and broad-leaved evergreen ...201423893311
differentiation of strains from the bacillus cereus group by rflp-pfge genomic fingerprinting.bacillus mycoides, bacillus pseudomycoides, bacillus weihenstephanensis, bacillus anthracis, bacillus thuringiensis, and bacillus cereus belong to the b. cereus group. the last three species are characterized by different phenotype features and pathogenicity spectrum, but it has been shown that these species are genetically closely related. the macrorestriction analysis of the genomic dna with the noti enzyme was used to generate polymorphism of restriction profiles for 39 food-borne isolates (b ...201323893780
antimicrobial activity and phytochemical constituents of leaf extracts of cassia auriculata.plants produce a wide variety of phytochemical constituents, which are secondary metabolites and are used either directly or indirectly in the pharmaceutical industry. 'for centuries, man has effectively used various components of plants or their extracts for the treatment of many diseases, including bacterial infections. in the present study methanol, chloroform and aqueous extracts of cassia auriculata leaf were subjected for antimicrobial activity by well-diffusion method against six bacteria ...201323901174
[diarrhoea and oedema in two show horses after feeding a pelleted supplemental feed for horses according to vdlufa's perspective of microbial quality classified as safe for use in horses].a new batch of a supplemental feed was fed as pellets (diameter 8 mm) to two warmblood-type horses. one horse developed watery diarrhoea within two days. pronounced oedema due to hypalbuminemia was seen about ten days later. the feed was replaced by pellets of identical composition and mixing process, but lower diameter (5 mm). after one week of feeding, oedema regressed and faeces were normally formed. at refeeding the larger sized pellets, the symptoms recurred, but now both horses were affect ...201323901590
screening of biological activities of polygonum maritimum l. from algerian coast.to investigate the antioxidant and the antibacterial activities of crude extract from aerial part of polygonum maritimum l. (polygonaceae) (p. maritimum) and to find new actives biomolecules.201323905017
draft genome sequence of bacillus cereus strain f, isolated from ancient permafrost.bacillus cereus strain f was isolated and cultured from a sample of permafrost, aged presumably about 3 million years, on the mammoth mountain (62°56'n, 133°59'e). these genome data provide the basis to investigate bacillus cereus f, identified as a long-term survivor of the extremely cold and close environment.201323908292
antimicrobial potential of flavoring ingredients against bacillus cereus in a milk-based beverage.natural ingredients--cinnamon, cocoa, vanilla, and anise--were assessed based on bacillus cereus vegetative cell growth inhibition in a mixed liquid whole egg and skim milk beverage (lwe-sm), under different conditions: ingredient concentration (1, 2.5, and 5% [wt/vol]) and incubation temperature (5, 10, and 22 °c). according to the results obtained, ingredients significantly (p<0.05) reduced bacterial growth when supplementing the lwe-sm beverage. b. cereus behavior was mathematically described ...201323909775
exploring the biosynthesis of unsaturated fatty acids in bacillus cereus atcc 14579: functional characterization of novel acyl-lipid desaturases.at low temperatures, bacillus cereus synthesizes large amounts of unsaturated fatty acids (ufas) with double bonds in positions δ5 and δ10, as well as δ5,10 di-unsaturated fatty acids. through sequence homology searches we identified two orfs encoding a putative δ5 desaturase and a fatty acid acyl-lipid desaturase in b. cereus atcc 14579 genome, named bc2983 and bc0400, respectively. functional characterization by means of heterologous expression in bacillus subtilis of orfs bc2983 and bc0400 co ...201323913431
phospholipase c from two bacterial strains acts differently on pure phospholipids and membrane bound glycosylphosphatidylinositol (gpi) anchors.phospholipase c (plc) was purified to homogeneity from the culture filtrate of bacillus cereus (65-fold, 540 u/mg protein) and b. thuringiensis (76-fold, 306 u/mg protein) by conventional techniques of enzyme purification. the purified enzymes have the molecular mass of 34 kda and 38 kda respectively, as determined by sds-page. both the plcs exhibited identical sensitivity to ph, temperature, cations, anions and inhibitors like glutathione and p-chloromercuribenzoate. plc-bc showed a preference ...200523923568
gram-positive siderophore-shuttle with iron-exchange from fe-siderophore to apo-siderophore by bacillus cereus yxeb.small molecule iron-chelators, siderophores, are very important in facilitating the acquisition of fe(iii), an essential element for pathogenic bacteria. many gram-negative outer-membrane transporters and gram-positive lipoprotein siderophore-binding proteins have been characterized, and the binding ability of outer-membrane transporters and siderophore-binding proteins for fe-siderophores has been determined. however, there is little information regarding the binding ability of these proteins f ...201323924612
enhanced antimicrobial activity of peptide-cocktails against common bacterial contaminants of ex vivo stored platelets.bacterial contamination of blood components such as ex vivo-stored platelets is a major safety risk in transfusion medicine. we have recently shown that synthetic antimicrobial peptides named pd1-pd4 derived from the thrombin-induced human platelet-derived antimicrobial proteins, and repeats of arg-trp (rw1-rw5) demonstrate microbicidal activity against selected bacteria and viruses. in the present study, we selected pd3, pd4, rw2, rw3 and rw4 and evaluated each individual peptide and their vari ...201423926880
bacillus cereus septicemia in a patient with acute lymphoblastic leukemia: a case report and review of the literature.bacillus cereus is an aerobic gram-positive, spore-forming, rod-shaped bacterium that is responsible for foodborne illnesses. we report on a 15-year-old girl with b-cell acute lymphoblastic leukemia, who fell into a somnolent state after presenting with a 12-hour history of fever, muscle soreness, myalgia in both calves, sore throat, and vomiting. fulminant septicemic syndrome caused by b. cereus was finally identified. the aim of this work is the introduction of b. cereus as a differential diag ...201623927823
summertime bacillus cereus colonization of hospital newborns traced to contaminated, laundered linen.routine screening of premature newborns for haemolytic streptococci, staphylococcus aureus and enteric gram-negative bacteria done at birth using umbilical swabs identified clustering of babies colonized with bacillus cereus in summers of 2009 and 2010 at a 400-bedded uk general hospital.201323927924
tuning of thioredoxin redox properties by intramolecular hydrogen bonds.thioredoxin-like proteins contain a characteristic c-x-x-c active site motif and are involved in a large number of biological processes ranging from electron transfer, cellular redox level maintenance, and regulation of cellular processes. the mechanism for deprotonation of the buried c-terminal active site cysteine in thioredoxin, necessary for dissociation of the mixed-disulfide intermediate that occurs under thiol/disulfide mediated electron transfer, is not well understood for all thioredoxi ...201323936007
kannurin, a novel lipopeptide from bacillus cereus strain ak1: isolation, structural evaluation and antifungal activities.this study was performed to isolate and characterize novel antifungal lipopeptide from bacillus cereus.201323937170
a computational module assembled from different protease family motifs identifies pi plc from bacillus cereus as a putative prolyl peptidase with a serine protease scaffold.proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. these distinct types have been systematically categorized in the merops database. while a blast search on these proteases identifies homologous proteins, sequence alignment methods often fail to identify relationships arising from convergent evolution, exon shuffling, and modular reuse of catalytic units. we have previously established a computational method to detect functions in proteins based on the spatial and elect ...201323940667
synthesis, sar and antibacterial activity of hybrid chloro, dichloro-phenylthiazolyl-s-triazines.a series of hybrid novel chloro (1a-9a) and dichloro (10b-18b) phenylthiazolyl-s-triazine were synthesized and subsequently subjected to their antibacterial activity against three gram positive viz. lactobacillus casei (ncim-2651); bacillus cereus (ncim-2458); staphylococcus aureus (ncim-2120) and three gram negative viz salmonella typhimurium (ncim-2501); escherichia coli (ncim-2065); klebsiella aerogenes (ncim-2098). the sar studies around the lead compound revealed that introduction of electr ...201223960775
isolation and screening of bioactive principle from chaetomorpha antennina against certain bacterial strains.microbial pathogens develop resistance to a particular antibiotic after repeated administration during the treatment of infectious diseases. moreover, multiple drug resistance is a very common problem especially in hospital acquired infections. therefore, it is necessary to find out alternative antibacterial drugs and the present trend is focused on seaweeds. this preliminary research work was carried out to find out the antibacterial activity of petroleum ether extract of chaetomorpha antennina ...201323960826
influence of inoculation with plant growth promoting rhizobacteria (pgpr) on tomato plant growth and nematode reproduction under greenhouse conditions.numerous species of soil bacteria which flourish in the rhizosphere of plants or around plant tissues stimulate plant growth and reduce nematode population by antagonistic behavior. these bacteria are collectively known as pgpr (plant growth promoting rhizobacteria). the effects of six isolates of pgpr pseudomonas putida, pseudomonas fluorescens, serratia marcescens, bacillus amyloliquefaciens, bacillus subtilis and bacillus cereus, were studied on tomato plant growth and root knot nematode repr ...201223961220
antioxidative, antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract.this study investigated the phytochemical, antioxidative, antimicrobial and cytotoxic effects of leea indica leaf ethanol extract. phytochemical values namely total phenolic and flavonoid contents, total antioxidant capacity, dpph radical scavenging effect, fecl3 reducing power, dmso superoxide scavenging effect and iron chelating effects were studied by established methods. antibacterial, antifungal and cytotoxic effects were screened by disk diffusion technique, food poison technique and brine ...201323961238
chitosan-containing bread made using marine shellfishery byproducts: functional, bioactive, and quality assessment of the end product.chitosan is nature's second most abundant polymer after cellulose and forms the structural support in crustacean shell material and basidomycete mushroom stalks. chitosan is a known antimicrobial agent but, to date, was not examined as an antimicrobial agent in bread formulations for the prevention of mold or rope formation. the aim of this work was to investigate the effects of chitosan generated from prawn shell byproducts on the color, moisture, and texture and crumb formation of bread. a sec ...201323962198
evaluation of direct 16s rdna sequencing as a metagenomics-based approach to screening bacteria in bottled water.deliberate or accidental contamination of food, feed, and water supplies poses a threat to human health worldwide. a rapid and sensitive detection technique that could replace the current labor-intensive and time-consuming culture-based methods is highly desirable. in addition to species-specific assays, such as pcr, there is a need for generic methods to screen for unknown pathogenic microorganisms in samples. this work presents a metagenomics-based direct-sequencing approach for detecting unkn ...201323971801
three different bacterial detection systems for platelet concentrates under inter-laboratory conditions.a variety of screening methods are currently used worldwide in order to decrease the risk of transfusion-transmitted sepsis and improve the safety of pcs.201323972777
addition of ethanol to supercritical carbon dioxide enhances the inactivation of bacterial spores in the biofilm of bacillus cereus.supercritical carbon dioxide (sc-co2) was used to inactivate bacillus cereus spores inside biofilms, which were grown on stainless steel. sc-co2 treatment was tested using various conditions, such as pressure treatment (10-30 mpa), temperature (35-60°c), and time (10-120 min). b. cereus vegetative cells in the biofilm were completely inactivated by treatment with sc-co2 at 10 mpa and at 35°c for 5 min. however, sc-co2 alone did not inactivate spores in biofilm even after the treatment time was e ...201323973829
characterization of aerobic spore-forming bacteria associated with industrial dairy processing environments and product spoilage.due to changes in the design of industrial food processing and increasing international trade, highly thermoresistant spore-forming bacteria are an emerging problem in food production. minimally processed foods and products with extended shelf life, such as milk products, are at special risk for contamination and subsequent product damages, but information about origin and food quality related properties of highly heat-resistant spore-formers is still limited. therefore, the aim of this study wa ...201323973839
the diversity and antimicrobial activity of preussia sp. endophytes isolated from australian dry rainforests.limited knowledge currently exists regarding species diversity and antimicrobial activity of endophytic isolates of preussia within australia. this report describes endophytic preussia species that were identified through molecular analysis of the internal transcribed spacer region. screening for antimicrobial secondary metabolites was determined by testing crude ethyl acetate (etoac) extracts derived from fungal mycelia against a panel of atcc type strains which included bacillus cereus, escher ...201423975673
cyclic dipeptides from rhabditid entomopathogenic nematode-associated bacillus cereus have antimicrobial activities.the cell free culture filtrate of bacillus cereus associated with an entomopathogenic nematode, rhabditis (oscheius) sp. exhibited strong antimicrobial activity. the ethyl acetate extract of the bacterial culture filtrate was purified by silica gel column chromatography to obtain four bioactive compounds. the structure and absolute stereochemistry of these compounds were determined based on extensive spectroscopic analyses (fabms, (1)h nmr, (13)c nmr, (1)h-(1)h cosy, (1)h-(13)c hmbc) and marfey' ...201423979826
synthesis, spectral, and in vitro antibacterial studies of organosilicon(iv) complexes with schiff bases derived from amino acids.the present work stems from our interest in the synthesis, characterization, and antibacterial evaluation of organosilicon(iv) complexes of a class of amino-acid-based schiff base which have been prepared by the interaction of ethoxytrimethylsilane with the schiff bases (n oh) in 1 : 1 molar ratio. these complexes have been characterized by elemental analysis, molar conductance, and spectroscopic studies including electronic ir and nmr ((1)h, (13)c, and (29)si) spectroscopy. the analytical and s ...201323983671
optimization of fermentation conditions for green pigment production from bacillus cereus m¹ 16 (mtcc 5521) and its pharmacological application.optimal culture conditions for the production of green pigment was investigated. the optimal culture condition for the production of an extracellular green pigment by growing bacillus cereus m(1) 16 (mtcc 5521) in a complex medium containing (g l(-1) ) peptone-4.0, beef extract-9.0, nacl-7.0, mgso4 .7h2 o-1.0 and kh2 po4 -5.0 was as follows ph-7.0 at 30°c for 72 h in a 5 l fermenter. aeration rate and agitator speed had no effect on the pigment production. thin layer chromatogram of the pigment ...201423992047
bacillus anthracis diagnostic detection and rapid antibiotic susceptibility determination using 'bioluminescent' reporter phage.genetically modified phages have the potential to detect pathogenic bacteria from clinical, environmental, or food-related sources. herein we assess an engineered 'bioluminescent' reporter phage (wß::luxab) as a clinical diagnostic tool for bacillus anthracis, the etiological agent of anthrax. wß::luxab is able to rapidly (within minutes) detect a panel of b. anthracis strains by transducing a bioluminescent phenotype. the reporter phage displays species specificity by its inability, or signific ...201323994352
Refresh
firstprev
...61626364656667686970...
nextlast
Displaying items 6701 - 6800 of 8187