Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| administration of killed bacteria together with listeriolysin o induces protective immunity against listeria monocytogenes in mice. | it is known that only listeriolysin o (llo)-producing listeria monocytogenes strains are able to induce protective immunity, but the underlining relationship between llo produced by virulent strains and generation of protective immunity in the infected host remains poorly understood. in the present study, it was found that llo gene expression was only detected in the mice infected with virulent strain which was able to induce protective immunity, while non-virulent strains or killed bacteria wer ... | 1998 | 9708181 |
| bacterial growth in ground beef patties made with meat from animals fed diets without or with supplemental vitamin e. | a study was designed to determine populations of aerobic bacteria, coliforms, sorbitol-negative bacteria, and listeria monocytogenes during display at 4 and 12 degrees c of ground beef patties made with meat from animals fed diets supplemented daily (for 100 days) with 0, 1,000, or 2,000 iu of vitamin e. the patties (113.5 g) were either left uninoculated or were inoculated with escherichia coli o157:h7 or l. monocytogenes and were tray-overwrapped and stored (at 4 or 12 degrees c for 8 to 10 or ... | 1998 | 9708250 |
| time and temperature of stretching as critical control points for listeria monocytogenes during production of mozzarella cheese. | different heating times and temperatures commonly used during curd stretching were investigated to determine their effects on the viability of listeria monocytogenes in mozzarella cheese. pasteurized whole milk was inoculated with two levels of l. monocytogenes (7 and 3 log cfu/g) and coagulated with citric acid and rennet. the curd was stretched at 55, 66, and 77 degrees c for 1, 3, and 5 min. results indicated that the majority of l. monocytogenes cells remained in the cheese curds at both ino ... | 1998 | 9708265 |
| inactivation of listeria innocua inoculated in liquid whole egg by high hydrostatic pressure. | the resistance of listeria innocua, as a model microorganism for listeria monocytogenes, to high hydrostatic pressure in liquid whole egg was studied at several pressures (300, 350, 400, and 450 mpa),temperatures (- 15, 2, and 20 degrees c), and times (5, 10, and 15 min). listeria innocus was added to liquid whole egg at approximately 10(6) cfu/ml. listeria innocua was not totally inactivated in any of the treatments. in general, reduction was better at 2 degrees than at room temperature, but th ... | 1998 | 9708266 |
| inhibition of listeria monocytogenes in a smear-surface soft cheese by lactobacillus plantarum whe 92, a pediocin ach producer. | the anti-listeria monocytogenes activity of lactobacillus plantarum whe 92, a pediocin ach producer, was investigated in munster cheese, a smear-surface soft cheese. the appearance of l. monocytogenes in the cheese, which naturally occurs solely in the crust and never before 1 week of ripening, could be prevented by spraying a cell suspension of l. plantarum whe 92 (ca. 10(5) cfu/ml) on the cheese surface at the beginning of the ripening period. l. monocytogenes was sometimes detected at low lev ... | 1998 | 9708279 |
| changes in populations of listeria monocytogenes inoculated on packaged fresh-cut vegetables. | a variety of wholesale and retail packaged vegetables and salads were inoculated with a mixture of strains of listeria monocytogenes and incubated at 4 and 10 degrees c. whole rutabagas, butternut squash, and onions, as well as packaged caesar salad, carrots, coleslaw mix, and stir-fry vegetables were purchased from local supermarkets in the ottawa area. l. monocytogenes population levels remained constant on all fresh-cut vegetables stored at 4 degrees c for 9 days, except for carrots and butte ... | 1998 | 9708280 |
| indicative and pathogenic microbiological quality of aquacultured finfish grown in different production systems. | the nature and number of indicator and pathogenic microbes in fish reared using recirculating and nonrecirculating water systems were compared. for each system, 20 samples of rainbow trout (oncorhynchus mykiss), tilapia (oreochromis spp.), hybrid striped bass (morone saxatilis x m. chrysops), and pacu (piaractus mesopotamicus) were randomly selected and gutted, and microbial analyses were performed using aoac procedures. five fish were subsampled and analyzed for indicative microbial quality wit ... | 1998 | 9708283 |
| studies on the risk assessment of listeria monocytogenes. | humans are frequently exposed to listeria monocytogenes, and high numbers may be ingested during consumption of certain types of food. however, epidemiological investigations show that listeriosis is a rare disease. risk assessment studies using an animal mouse model indicate that almost all l. monocytogenes serovars present in food have clear virulent properties. the intravenous dose causing infection in 50% (iv id50) of mice not previously exposed to l. monocytogenes (nonprotected mice) was 1. ... | 1998 | 9708290 |
| survival of bacterial pathogens in pasteurized process cheese slices stored at 30 degrees c. | six lots of commercial pasteurized process cheese slices were evaluated for the ability to support the growth of four foodborne pathogens, listeria monocytogenes, staphylococcus aureus, salmonella serotypes, and escherichia coli o157:h7, during 4 days of storage at 30 degrees c. individual cheese slices were inoculated separately with each pathogen to yield ca. 10(3) cfu/g. slices were packaged in sterile plastic sample bags and stored at 30 degrees c for up to 96 h. population of salmonella ser ... | 1998 | 9708298 |
| fate of bacterial pathogens and indicator organisms in liquid sweeteners. | the survival of pathogenic and indicator microorganisms in liquid sweeteners was studied. seven sweeteners--liquid sucrose, 42% high-fructose corn syrup (hfcs), 55% hfcs, 25 de (dextrose equivalent) corn syrup (cs), 36 de cs, 63 de cs, 50% medium invert sucrose, and 65% high-maltose corn syrup (hmcs) were inoculated with salmonella spp., listeria monocytogenes, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and coliforms at a level of 10(5) cells per g. the inoculated products ... | 1998 | 9708299 |
| inhibition of listeria monocytogenes and aeromonas hydrophila by plant extracts in refrigerated cooked beef. | refrigerated ready-to-eat foods are becoming increasingly popular but are often vulnerable to contamination and subsequent growth by psychrotrophic foodborne pathogens. consequently, there is a need for additional methods to assure the safety of these foods. beef slices prepared from roasted whole sirloin tips were used in the study. nine plant extracts were evaluated for ability to inhibit the growth of two psychrotrophic pathogens (aeromonas hydrophila and listeria monocytogenes) in refrigerat ... | 1998 | 9708301 |
| growth of inoculated psychrotrophic pathogens on refrigerated fillets of aquacultured rainbow trout and channel catfish. | aquacultured rainbow trout (oncorhynchus mykiss) and channel catfish (ictalurus punctatus) fillets were inoculated with the psychrotrophic pathogens listeria monocytogenes and aeromonas hydrophila: cell populations were monitored during refrigerated storage at 2 to 4 degrees c. fillets of both species were placed individually in sterile plastic bags and inoculated with cell suspensions (10(4.7) cfu/100 g of fish) of either a. hydrophila or l monocytogenes or of both a. hydrophila and l. monocyto ... | 1998 | 9708302 |
| the incidence of listeria spp., salmonella spp., and clostridium botulinum in smoked fish and shellfish. | the frequency of occurrence of listeria spp., salmonella spp., and clostridium botulinum is samples of smoked finfish and smoked shellfish was analyzed over a 5-year period. listeria monocytogenes were isolated from 14% of 1,080 samples. for those samples where the smoke process was known, the incidence of l. monocytogenes was higher in cold-smoked than hot-smoked products (51 of 240 cold-smoked compared to 19 of 215 hot-smoked products). listeria species other than l. monocytogenes were also de ... | 1998 | 9708303 |
| incidence of listeria monocytogenes in cheese produced in rio de janeiro, brazil. | the present study evaluated the incidence of listeria spp. in some brazilian cheeses obtained from retail stores in rio de janeiro, of 103 samples of various types of cheese examined as recommended in the listeria isolation protocol of the health protection branch of canada, 11 (10.68%) were contaminated by listeria monocytogenes, 13 (12.62%) by listeria innocua, 6 (5.83%) by listeria grayi, and 1 (0.97%) by listeria welshimeri. a higher incidence of l. monocytogenes as observed mainly in the ho ... | 1998 | 9708310 |
| listeriosis in pediatric oncology patients. | adult cancer patients are considered to be at an increased risk for listeria monocytogenes infections, but, to the authors' knowledge, little information regarding this infection in the pediatric oncology population has been published. | 1998 | 9708951 |
| interaction of hydrostatic pressure, time and temperature of pressurization and pediocin ach on inactivation of foodborne bacteria. | high hydrostatic pressure, because it can kill microorganisms, is being investigated for potential use as a nonthermal food preservation method. the objective of this study was to determine the hydrostatic pressurization parameters, pressure, time, and temperature, and a bacteriocin that in combination would destroy 7 to 8 log cycles of pathogenic and spoilage bacterial populations. we suspended cells of staphylococcus aureus, listeria monocytogenes, salmonella typhimurium, escherichia coli o157 ... | 1998 | 9709205 |
| microbiological quality of australian beef carcass meat and frozen bulk packed beef. | two studies were undertaken to determine the microbiological of beef carcass meat and frozen boneless bulk packed beef produced in australia. samples were collected from 1,063 beef carcasses and from 929 cartons of frozen boneless bulk packed beef over a period of approximately 12 months. samples were collected from works processing beef carcasses for the australian domestic market and from works targeting export markets. on carcasses processed for export markets, where bacterial counts were obt ... | 1998 | 9709207 |
| duplex (thermotroph-psychrotroph) quadrant plates: convenient, error-avoiding tools for monitoring of haccp-contained food lines and for epidemiological investigations under conditions of military or other constraints. | a set of two "two-tier" (thermotroph-psychrotroph) single quadrant plates (qps) was developed previously to allow convenient enumeration of numbers of colony-forming units of most pertinent pathogenic bacteria or marker bacteria in foods. these include listeria monocytogenes, staphylococcus aureus, bacillus cereus, thermotrophic and psychrotrophic enterobacteriaceae, clostridium spp., and enterococcus spp. as the qps had given excellent results in monitoring samples of marketed food products pot ... | 1998 | 9709209 |
| an efficient sampling technique used to detect four foodborne pathogens on pork and beef carcasses in nine belgian abattoirs. | the method presented in this paper should prove useful in assessing the effectiveness of haccp plans developed in slaughterhouses. samples were collected by swabbing well-defined areas of pork and beef carcasses with sterile gauze. between 160 and 420 half-carcasses were swabbed in each of nine pork or beef slaughterhouses. swabs from five carcasses were placed in the same sterile stomacher bag, constituting a single composite sample. standard or validated analytical methods were used to isolate ... | 1998 | 9709223 |
| growth and survival of listeria monocytogenes in vacuum-packaged ground beef inoculated with lactobacillus alimentarius floracarn l-2. | a culture of the psychotrophic strain floracarn l-2 of lactobacillus alimentarius was added to ground beef (ph 5.4) inoculated with two isolates of listeria monocytogenes able to grow in refrigerated ground beef. the ground beef was vacuum-packaged and stored for 9 weeks at 4 degrees c. populations of inoculated l. monocytogenes initially were 6.3 to 6.4 log10 cfu/g and increased to 7.4 log10 cfu/g in ground beef with no added lactobacilli. addition of l. alimentarius l-2 or its antibiotic-resis ... | 1998 | 9709226 |
| recovery of foodborne microorganisms from potentially lethal radiation damage. | a two-stage recovery protocol was examined for microorganisms following gamma irradiation in phosphate buffer at 0 degrees c. in the first stage, survivors were recovered on basal yeast extract agar and held at various temperatures suboptimal for their growth for 20 h (resuscitation protocol). in the second stage the survivors were incubated for an additional 24 h, but in this case at their optimum temperature for growth. controls consisted of survivors which were not subjected to the resuscitat ... | 1998 | 9709232 |
| heat resistance and fatty acid composition of listeria monocytogenes: effect of ph, acidulant, and growth temperature. | the objective of this study was to determine the influence of ph, acidulant, and growth temperature history on the heat resistance and fatty acid composition of listeria monocytogenes scott a. cells were grown to late exponential phase (od600 = 0.6) at 10, 19, or 37 degrees c in brain heart infusion broth acidified to ph 5.4 or 7 with either acetic or lactic acid. thermal death times at 60 degrees c subsequently were determined by using a submerged-coil heating apparatus. the surviving cell popu ... | 1998 | 9709249 |
| chemical and bacteriological characteristics of pichtogalo chanion cheese and mesophilic starter cultures for its production. | sixty-two samples of pichtogalo chanion cheese traditionally produced in crete, a few (3 to 6) days old, were analyzed for some chemical and bacteriological characteristics. the results of physicochemical analyses were as follows: (1) moisture content 61.63% (standard deviation 4.67); (2) fat in dry matter 54.03% (sd 7.73); (3) protein content 14.23% (sd 1.72); (4) salt content 1.02% (sd 0.38); (5) water activity (aw) 0.990 (sd 0.003); and (6) ph 4.36 (sd 0.25). none of the samples yielded salmo ... | 1998 | 9709250 |
| effect of several decontamination procedures on listeria monocytogenes growing in biofilms. | listeria monocytogenes is a pathogenic bacterium which has been implicated in several foodborne illnesses. this microorganism grows into biofilms attached to the surfaces in food-processing plants, increasing its resistance to antimicrobial agents. the present work was realized to investigate the attachment of l. monocytogenes isolates to glass surfaces and to find a decontamination procedure to remove these bacteria in biofilms. three-day biofilms were prepared by growing l. monocytogenes isola ... | 1998 | 9709259 |
| human macrophages induced in vitro by macrophage colony-stimulating factor are deficient in il-12 production. | il-12 is important for th1 differentiation. myeloid-derived antigen-presenting cells (apc) such as monocytes, macrophages (mphi) and dendritic cells (dc) are believed to be major sources of il-12 in vivo. we have compared il-12 production of fresh monocytes with mphi differentiated in vitro using macrophage colony-stimulating factor (m-csf) or human plasma, and in vitro generated dendritic cells, since these differentiated cell types represent apc at sites of antigen challenge. macrophages stimu ... | 1998 | 9710227 |
| survival of bioluminescent listeria monocytogenes and escherichia coli o157:h7 in soft cheeses. | pasteurized and raw milks that had been inoculated at 10(4) cfu/ml with bioluminescent strains of listeria monocytogenes and escherichia coli o157:h7 were used in the manufacture of camembert and feta cheeses with or without nisin-producing starter culture. survival of both organisms was determined during the manufacture and storage of camembert and feta cheeses at 2 +/- 1 degree c for 65 and 75 d, respectively. bacterial bioluminescence was used as an indicator to enumerate the colonies plated ... | 1998 | 9710748 |
| listeria monocytogenes in the colon in a case of fulminant ulcerative colitis. | a case of ulcerative colitis in which the presence of listeria monocytogenes was confirmed in the resected colon with polymerase chain reaction and subsequent southern blot analysis and immunohistochemistry using antibody against listeria is presented. the patient developed ulcerative colitis at the age of 59 years. prednisolone, 50 mg/day, was given for severe ulcerative colitis. later the disease became fulminating, indicating colectomy 4 months after the onset. multiple sealed colonic perfora ... | 1998 | 9712246 |
| effect of siderophores, catecholamines, and catechol compounds on listeria spp. growth in iron-complexed medium. | almost all bacteria require iron for growth and virulence expression. however, listeria spp. do not produce any siderophore for iron acquisition. representative strains of each of the six species of listeria were examined for their ability to use various compounds as iron suppliers in iron-restricted medium. here we show that l. monocytogenes, l. innocua, l. ivanovii, l. welshimeri, l. seeligeri, and l. grayi were able to use exogenous siderophores and various catechol ligands, including catecho ... | 1998 | 9712730 |
| the contributions of reactive oxygen intermediates and reactive nitrogen intermediates to listericidal mechanisms differ in macrophages activated pre- and postinfection. | the contribution of reactive oxygen intermediates (roi) and reactive nitrogen intermediates (rni) to the killing of listeria monocytogenes by macrophages activated by addition of spleen cells from listeria-immune mice plus specific antigen was examined. when macrophages were infected with l. monocytogenes and then spleen cells were added, there was not as big a difference in listericidal activity between macrophages cultured with normal spleen cells and those cultured with immune spleen cells as ... | 1998 | 9712745 |
| fas (cd95)-dependent cell-mediated immunity to listeria monocytogenes. | two distinct and complementary pathways, one mediated by perforin and the other dependent upon cd95 (fas), effect cell-mediated cytotoxicity. we examined the relative roles of these pathways in host defenses against the intracellular bacterial pathogen listeria monocytogenes by using murine listeriosis as a model system. mice which lacked both perforin and fas (p0l0) were generated, and their responses to primary and secondary listeriosis were compared to those of wild-type (wt), fas-deficient ( ... | 1998 | 9712760 |
| entry of listeria monocytogenes into neurons occurs by cell-to-cell spread: an in vitro study. | listeria monocytogenes is an intracellular pathogen that causes severe central nervous system infection in humans and animals. the ability of this bacterium to penetrate nerve cells was investigated by using rat spinal cell cultures. entry into distinct cell types, i. e., glial cells and neurons, was monitored by a differential immunofluorescence technique with antibodies against cell type-specific markers and the bacterial pathogen. l. monocytogenes was detected predominantly within macrophages ... | 1998 | 9712801 |
| fate of gamma-irradiated listeria monocytogenes during refrigerated storage on raw or cooked turkey breast meat. | the radiation resistance and ability of listeria monocytogenes atcc 7644, 15313, 43256, and 49594 to multiply on irradiated, air-packed, refrigerated raw or cooked turkey breast meat nuggets (ca. 25 g) and ground turkey breast meat was investigated. gamma-radiation d values for l. monocytogenes were significantly different on raw and cooked nuggets, 0.56 +/- 0.03 kgy and 0.69 +/- 0.03 kgy, respectively; but they were not significantly different (p < or = 0.05) on raw and cooked ground turkey mea ... | 1998 | 9713758 |
| listeria monocytogenes-associated acute hepatitis in a liver transplant recipient. | we report a case of listeria monocytogenes bacteriemia with liver involvement mimicking acute viral hepatitis in a liver transplant recipient. the patient presented with fever, jaundice and alanine aminotransferase levels ten times the upper limit of normal. liver biopsy showed signs of acute hepatitis and occasional granulomas. both blood and liver biopsy cultures were positive for listeria monocytogenes. the patient received a 3 week course of ampicillin and gentamicin with clinical and bioche ... | 1998 | 9716234 |
| reduced intracellular activity of antibiotics against listeria monocytogenes in multidrug resistant cells. | multidrug resistance is expressed not only by bacteria, but also by tumor cells and by some normal cells of the body. it enables eukaryotic cells to exclude not only cytostatic drugs but also non-cytostatic antibiotics. this was demonstrated in genetically engineered multidrug resistant (mdr) cells infected with the facultative intracellular bacterium listeria monocytogenes for all macrolide antibiotics tested (azithromycin, clarithromycin, erythromycin, josamycin, roxithromycin and spiramycin). ... | 1998 | 9716288 |
| interactions of the bacterial pathogen listeria monocytogenes with mammalian cells: bacterial factors, cellular ligands, and signaling. | listeria monocytogenes is a food borne pathogen which has the very unique property of crossing three barriers during infection eliciting meningitis, meningo-encephalitis and abortions with a mortality rate of about 30%. indeed, after crossing the intestinal barrier, listeria disseminates via the lymph and the blood, to the brain and/or the placenta after crossing the brain-blood barrier and/or the placental barrier. during disease, this organism infects a variety of tissues and cell types in whi ... | 1998 | 9717257 |
| reduction of caecal listeria monocytogenes in leghorn chicks following treatment with a competitive exclusion culture (preempt) | day-of-hatch leghorn chicks were treated by oral gavage with preempt, a continuous-flow competitive exclusion culture containing broiler caecal bacteria, followed by an oral challenge with listeria monocytogenes, to determine the effects of preempt on l. monocytogenes caecal colonization. increased (p < 0.001) concentrations of caecal propionic acid in control chicks compared with preempt-treated chicks at 3 days of age were indicative of the establishment of the preempt bacteria. caeca from con ... | 1998 | 9717314 |
| macrophages and hepatocytic cells as chemokine producers in murine listeriosis. | the major target organ of systemic infection with the intracellular bacterium listeria monocytogenes is the liver, to where inflammatory leukocytes are rapidly recruited. we determined by reverse transcriptase polymerase chain reaction the early chemokine response in the liver after systemic infection of mice with listeriae, and in parallel compared chemokine release from macrophages and hepatocytic cells in vitro. murine bone marrow-derived macrophages (bmm) grown in fetal calf serum-supplement ... | 1998 | 9717670 |
| successful treatment of murine listeriosis and salmonellosis with levofloxacin. | levofloxacin (l-ofloxacin) is a fluoroquinolone derivative. it is the active substance contained in ofloxacin with a broad spectrum of antibacterial activity against both gram-negative and gram-positive bacteria. in this work we examined the activity of levofloxacin against the facultative intracellular bacteria listeria monocytogenes and salmonella typhimurium in vitro, in tissue culture and in animal models of infection. the minimum inhibitory concentrations (mics) mic90 for salmonella enteric ... | 1998 | 9720471 |
| identification of the gene encoding the alternative sigma factor sigmab from listeria monocytogenes and its role in osmotolerance. | listeria monocytogenes is well known for its robust physiology, which permits growth at low temperatures under conditions of high osmolarity and low ph. although studies have provided insight into the mechanisms used by l. monocytogenes to allay the physiological consequences of these adverse environments, little is known about how these responses are coordinated. in the studies presented here, we have cloned the sigb gene and several rsb genes from l. monocytogenes, encoding homologs of the alt ... | 1998 | 9721294 |
| inhibition of listeria monocytogenes by plantaricin na, an antibacterial substance from lactobacillus plantarum. | a lactobacillus plantarum of vegetable origin produced a bacteriocin inhibitor to listeria monocytogenes. the antimicrobial agent was inactivated by proteolytic enzymes, was resistant to heat (100 degrees c for 30 min) and stable over a wide ph range (ph 2-10), and displayed a bactericidal mode of action. growth inhibition of l. monocytogenes depend on bacteriocin concentration. the antilisterial efficiency depended on the strain of l. monocytogenes used but was not influenced by the growth phas ... | 1998 | 9721606 |
| antibiotic use in neonatal sepsis. | neonatal sepsis is a life-threatening emergency and any delay in treatment may cause death. initial signs of neonatal sepsis are slight and nonspecific. therefore, in suspected sepsis, two or three days empirical antibiotic therapy should begin immediately after cultures have been obtained without awaiting the results. antibiotics should be reevaluated when the results of the cultures and susceptibility tests are available. if the cultures are negative and the clinical findings are well, antibio ... | 1998 | 9722468 |
| listeria monocytogenes as a probe to study cell-mediated immunity. | the intracellular bacterium listeria monocytogenes continues to serve as a model to define general paradigms of cell-mediated immunity. genetic manipulations of the bacterium and its murine host have allowed us to begin dissecting the intricate interactions between this bacterium and the immune system. as a result, we have gained new insights into the mechanisms of immune surveillance, achieved better understanding of bacterial tactics for immune evasion and developed novel strategies in vaccine ... | 1998 | 9722922 |
| minimum inhibitory concentration of smoke wood extracts against spoilage and pathogenic micro-organisms associated with foods. | antimicrobial activity of seven commercial smoke preparations (four liquid and three solid) was studied. the minimum inhibitory concentration (mic) was determined against a selection of food spoilage and pathogenic micro-organisms. the main smoke components were identified and quantified by gas chromatography/mass spectrometry. the most effective condensate was s2. all strains except salmonella enteritidis were inhibited by s2 with an mic < 0.5-1.5%. smoke extract l2 inhibited growth of vibrio v ... | 1998 | 9722997 |
| [rhomboencephalitis caused by listeria monocytogenes: a case studies with magnetic nuclear resonance]. | central nervous system infections due to listeria monocytogenes result in a variety of clinical syndromes ranging from meningitis to rhomboencephalitis. we report the case of a previously healthy patient with rhomboencephalitis in whom the ct scan was normal, while magnetic resonance imaging (mri) confirmed the diagnosis. we review the literature and emphasize the value of mri for timely diagnosis. | 1991 | 9723100 |
| sequence profile of the parallel beta helix in the pectate lyase superfamily. | the parallel beta helix structure found in the pectate lyase superfamily has been analyzed in detail. a comparative analysis of known structures has revealed a unique sequence profile, with a strong positional preference for specific amino acids oriented toward the interior of the parallel beta helix. using the unique sequence profile, search patterns have been constructed and applied to the sequence databases to identify a subset of proteins that are likely to fold into the parallel beta helix. ... | 1998 | 9724624 |
| bacterial surface proteins recognized by cd4+ t cells during murine infection with listeria monocytogenes. | optimal immunity to the gram-positive pathogen listeria monocytogenes (lm) requires both cd8+ and cd4+ antigen-specific t cell responses. understanding how cd4+ t cells function in an immune response to lm and how bacterial proteins are processed to peptide/mhc class ii complexes in infected cells requires identification of these proteins. using lacz-inducible, lm-specific cd4+ t cells as probes, we identified two immunogenic lm proteins by a novel expression cloning strategy. the antigenic pept ... | 1998 | 9725229 |
| the role of the bacterial membrane protein acta in immunity and protection against listeria monocytogenes. | acta, an essential virulence factor of listeria monocytogenes, is an integral membrane protein that is required for intracellular motility, cell-to-cell spread, and rapid dissemination of the bacteria in the infected host. to reveal cytotoxic t cell responses against acta we introduced a recombinant soluble form of acta into the mhc class i-processing compartment of apc using a variant of listeriolysin mutated within its immunodominant mhc class i epitope. with this experimental system we demons ... | 1998 | 9725238 |
| cpg dna induces sustained il-12 expression in vivo and resistance to listeria monocytogenes challenge. | vertebrates have evolved innate immune defense mechanisms that recognize and respond to structural patterns that are specific to microbial molecules. one such pattern recognition system is based on unmethylated cpg dinucleotides in particular sequence contexts (cpg motifs); these motifs are common in bacterial dna but are under-represented ("cpg suppression") and methylated in vertebrate dna. mice that are injected with bacterial dna or synthetic oligodeoxynucleotides (odns) containing cpg motif ... | 1998 | 9725240 |
| cloning and expression of the listeria monocytogenes scott a ptsh and ptsi genes, coding for hpr and enzyme i, respectively, of the phosphotransferase system. | the phosphoenolpyruvate (pep)-dependent phosphotransferase system (pts) utilizes high-energy phosphate present in pep to drive the uptake of several different carbohydrates in bacteria. in order to examine the role of the pts in the physiology of listeria monocytogenes, we identified the ptsh and ptsi genes encoding the hpr and enzyme i proteins, respectively, of the pts. nucleotide sequence analysis indicated that the predicted proteins are nearly 70% similar to hpr and enzyme i proteins from o ... | 1998 | 9726852 |
| modeling of the competitive growth of listeria monocytogenes and lactococcus lactis in vegetable broth. | current mathematical models used by food microbiologists do not address the issue of competitive growth in mixed cultures of bacteria. we developed a mathematical model which consists of a system of nonlinear differential equations describing the growth of competing bacterial cell cultures. in this model, bacterial cell growth is limited by the accumulation of protonated lactic acid and decreasing ph. in our experimental system, pure and mixed cultures of lactococcus lactis and listeria monocyto ... | 1998 | 9726854 |
| comparative studies of class iia bacteriocins of lactic acid bacteria. | four class iia bacteriocins (pediocin pa-1, enterocin a, sakacin p, and curvacin a) were purified to homogeneity and tested for activity toward a variety of indicator strains. pediocin pa-1 and enterocin a inhibited more strains and had generally lower mics than sakacin p and curvacin a. the antagonistic activity of pediocin-pa1 and enterocin a was much more sensitive to reduction of disulfide bonds than the antagonistic activity of sakacin p and curvacin a, suggesting that an extra disulfide bo ... | 1998 | 9726871 |
| characterization of a bacteriocin produced by streptococcus thermophilus 81. | a new bacteriocin, produced by streptococcus thermophilus 81 has been isolated, purified and characterized. by its heat sensitivity and broad inhibitory spectrum it does not resemble any other s. thermophilus bacteriocin. the mode of action is bacteriostatic. this peptide of 32 amino acids is efficient against several bacillus species, listeria monocytogenes, salmonella typhimurium, escherichia coli, yersinia pseudotuberculosis and yersinia enterocolitica. this bacteriocin is heat labile but its ... | 1998 | 9728685 |
| a comparison of listeria monocytogenes serovar 4b isolates of clinical and food origin in japan by pulsed-field gel electrophoresis. | pulsed-field gel electrophoresis (pfge) patterns of 102 l. monocytogenes serovar 4b isolates from patients and foods examined in japan were compared with 16 isolates from foodborne listeriosis episodes which occurred in north america or europe. using a combination of pfge patterns with the restriction enzymes smai, apai, asci and sse8387i, 82 clinical isolates from japan were categorized into 45 pfge types: the largest group of 17 isolates (20.7%) were of the same pfge type as cultures from the ... | 1998 | 9728691 |
| a rapid method for the identification and partial serotyping of listeria monocytogenes in food by pcr and restriction enzyme analysis. | two highly specific primers for listeria monocytogenes were used to yield from foods such as milk, soft cheese and meat, pcr products that were cleaved with the restriction enzyme hindiii. the fragments generated allowed a distinction between two groups of l. monocytogenes serovars: serovars 1/2a and 1/2c cluster in one group and serovars 1/2b, 3b and 4b in the other subgroup. since this procedure can be completed in 24 h, an epidemiological association between human disease and suspected source ... | 1998 | 9728692 |
| identification of cofilin, coronin, rac and capz in actin tails using a listeria affinity approach. | actin assembly is involved in cell motility and intracellular movement of listeria monocytogenes. induction of listeria actin tails is mediated by the surface protein acta. the n-terminal domain of acta is sufficient for this function. cell components known to play a role in the actin-based motility of listeria are vasp (vasodilatator-stimulated phosphoprotein), the multiprotein arp2/3 complex and cofilin. vasp interacts with the central domain of acta. proteins interacting with the n-terminal d ... | 1998 | 9730980 |
| host resistance against listeria monocytogenes is reciprocal during the course of infection in alymphoplastic aly mutant mice. | the aly is a unique spontaneous autosomal recessive mutation in mice that causes a systemic defect of lymph nodes and peyer's patches. we investigated host resistance against listeria monocytogenes infection in the mutant. the 50% lethal dose of l. monocytogenes in aly/aly mice was 10-fold higher than their heterozygotes, termed aly/+mice, or their wild-type c57bl/6 mice. the bacterial growth in the spleens and livers of aly/aly mice was more efficient early in infection, and their listericidal ... | 1998 | 9732696 |
| spontaneous bacterial peritonitis caused by infection with listeria monocytogenes: a case report and review of the literature. | spontaneous bacterial peritonitis is a frequent and often serious complication of long-standing ascites in the presence of advanced liver disease. coliform bacteria account for the infection in most cases and are thought to be related to translocation of bacteria from the bowel into the peritoneal cavity. the empiric use of cefotaxime is well established as most of the causative organisms are sensitive to this antibiotic. however, we report on a case of spontaneous bacterial peritonitis in a pat ... | 1998 | 9732945 |
| listeria monocytogenes meningitis in a penicillin-allergic paediatric renal transplant patient. | currently in many centres the extended spectrum cephalosporins (e.g. cefotaxime and ceftriaxone) are being used empirically for patients with suspected bacterial meningitis. we present a case of meningitis in a penicillin allergic paediatric renal transplant patient from whose cerebrospinal fluid (csf) listeria monocytogenes was cultured, despite four days of cefotaxime therapy. the patient was successfully treated with meropenem but required neuro-endoscopic intervention for hydrocephalus. | 1998 | 9733389 |
| a novel bacteriocin with a ygngv motif from vegetable-associated enterococcus mundtii: full characterization and interaction with target organisms. | a novel broad-spectrum antimicrobial peptide produced by vegetable-associated enterococcus mundtii was purified and characterized, and designated mundticin. to our knowledge, this is the first report on bacteriocin production by this organism. the elucidation of the full primary amino acid sequence of mundticin (kyygngvscnkkgcsvdwgkaigiignnsaanlatggaagwsk) revealed that this antimicrobial peptide belongs to the class iia bacteriocins of lactic acid bacteria which share a highly conserved n-termi ... | 1998 | 9733915 |
| characterization of listeria monocytogenes isolated from channel catfish (ictalurus punctatus). | to characterize listeria monocytogenes from tissues of channel catfish for their ability to cause hemolysis and grow intracellularly in mouse macrophages. | 1998 | 9736389 |
| activities of ll-37, a cathelin-associated antimicrobial peptide of human neutrophils. | human neutrophils contain two structurally distinct types of antimicrobial peptides, beta-sheet defensins (hnp-1 to hnp-4) and the alpha-helical peptide ll-37. we used radial diffusion assays and an improved national committee for clinical laboratory standards-type broth microdilution assay to compare the antimicrobial properties of ll-37, hnp-1, and protegrin (pg-1). although generally less potent than pg-1, ll-37 showed considerable activity (mic, <10 microgram/ml) against pseudomonas aerugino ... | 1998 | 9736536 |
| actin cytoskeleton: the arp2/3 complex gets to the point. | actin filament polymerization results primarily from the addition of monomers to pre-existing filaments. recent studies have revealed that the arp2/3 protein complex, which includes two actin-related proteins, can nucleate new actin filaments, and this capacity can be enhanced by acta, a protein used by listeria to polymerize actin. | 1998 | 9740796 |
| apolipoprotein e-deficient mice have impaired innate immune responses to listeria monocytogenes in vivo. | apolipoprotein e (apoe) influences both innate and acquired immunity in cultured cells. to determine whether apoe affects the immune system in vivo, listeria monocytogenes (lm) was administered intraperitoneally (10(4) c.f.u.) to congenic c57bl/6 apoe-/- and +/+ mice (n = 12 in each group). survival was assessed daily for 5 days. deficiency of apoe significantly increased death by day 5 (p = 0.03). the majority of deaths occurred at day 4. extent of infection after lm administration was assessed ... | 1998 | 9741685 |
| functional gene transfer from intracellular bacteria to mammalian cells. | we provide evidence of direct transfer of functional dna from bacteria to mammalian cells. an escherichia coli k12 diaminopimelate auxotroph made invasive by cloning the invasin gene from yersinia pseudotuberculosis transfers dna after simple co-incubation, into a variety of mammalian cell lines. transfer efficiency was enhanced in some cells by coexpression of the gene for listeriolysin from listeria monocytogenes. expression of the acquired genes occurs in both dividing and quiescent cells. th ... | 1998 | 9743121 |
| the mhc class i-restricted immune response to hiv-gag in balb/c mice selects a single epitope that does not have a predictable mhc-binding motif and binds to kd through interactions between a glutamine at p3 and pocket d. | using a strain of listeria monocytogenes that stably expresses and secretes hiv gag to deliver this ag to the mhc class i pathway of ag processing, we have identified the immunodominant ctl epitope to gag in the balb/c mouse and shown that it is kd restricted. the specific motif for the peptides that bind the mhc class i molecule h-2 kd is believed to be a nonamer with residues tyrosine or phenylalanine in the second amino acid position and leucine or isoleucine in the carboxyl-terminal or ninth ... | 1998 | 9743362 |
| two distinct phospholipases c of listeria monocytogenes induce ceramide generation, nuclear factor-kappa b activation, and e-selectin expression in human endothelial cells. | infection of endothelial cells by listeria monocytogenes is an essential step in the pathogenesis of listeriosis. we recently reported that l. monocytogenes induces up-regulation of e-selectin and other endothelial adhesion molecules and subsequent polymorphonuclear leukocyte (pmn) adhesion into cultured human endothelial cells. in the present study, we characterized the mechanisms of enhanced e-selectin expression using l. monocytogenes wild type (egd), the isogenic in-frame deletion mutants fo ... | 1998 | 9743365 |
| modulation of enzymatic activity and biological function of listeria monocytogenes broad-range phospholipase c by amino acid substitutions and by replacement with the bacillus cereus ortholog. | the secreted broad-range phosphatidylcholine (pc)-preferring phospholipase c (pc-plc) of listeria monocytogenes plays a role in the bacterium's ability to escape from phagosomes and spread from cell to cell. based on comparisons with two orthologs, clostridium perfringens alpha-toxin and bacillus cereus plc (plcbc), we generated pc-plc mutants with altered enzymatic activities and substrate specificities and analyzed them for biological function in tissue culture and mouse models of infection. t ... | 1998 | 9746585 |
| synergistic antibacterial action of heat in combination with nisin and magainin ii amide. | lactobacillus plantarum has been exposed to mild heat at temperatures between 48 and 56 degrees c in combination with low concentrations of the lantobiotic nisin in different sequential set-ups. exposure to heat and nisin caused synergistic reductions of lact. plantarum viability. efficient antimicrobial action was dependent on the growth state of the culture as well as on levels and sequences of treatment applications. listeria monocytogenes and escherichia coli were treated at 55 degrees c in ... | 1998 | 9750279 |
| antilisterial activity of enterocin 81, a bacteriocin produced by enterococcus faecium whe 81 isolated from cheese. | enterocin 81, a bacteriocin produced by enterococcus faecium whe 81 previously isolated from cheese, exhibited a very narrow spectrum of activity, which is mainly directed against enterococci and listeria spp. including listeria monocytogenes. enterocin 81 activity, which was extremely rapid with maximal effect achieved within 30 min, could not be detected after treatment with various proteolytic enzymes. this activity was bactericidal in nature and induced an important efflux of intracellular m ... | 1998 | 9750282 |
| survival of listeria monocytogenes in sea water and effect of exposure on thermal resistance. | survival, recoverability and sublethal injury of two strains of listeria monocytogenes, scott a and an environmental strain km, on exposure to sea water at 12.8 or 20.8 degrees c was determined using in situ diffusion chambers. plate counts were used to assess recoverability and injury while 5-cyano-2,3-ditolyl tetrazolium chloride (ctc) reduction was used to determine respiratory activity. t90 values (times for 10-fold decreases in numbers of recoverable cells) on non-selective medium (tryptica ... | 1998 | 9750285 |
| detection and characterization of a novel antibacterial substance produced by a lactobacillus delbrueckii strain 1043. | a novel antibacterial substance produced by a strain isolated from bulgarian yellow cheese was characterized. the producer strain was identified by molecular typing to belong to the species lactobacillus delbrueckii, which is a rare producer of bacteriocins. the inhibitory agent was heat stable and active against lactic acid bacteria species and several food-borne pathogens: listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, escherichia coli, yersinia enterocolitica and y. pse ... | 1998 | 9750290 |
| bacteriocins inhibit glucose pep:pts activity in listeria monocytogenes by induced efflux of intracellular metabolites. | glucose transport by the phosphoenolpyruvate (pep)-dependent phosphotransferase system (pts) of listeria monocytogenes is inhibited by the bacteriocins, nisin, pediocin jd and leuconocin s. to investigate the mechanism of inhibition, pts activity assays were performed with permeabilized, bacteriocin-treated l. monocytogens scott a cells. in the presence of exogenous pep, nisin stimulated the pts while both pediocin jd and leuconocin s partially inhibited its activity. these results suggested tha ... | 1998 | 9750302 |
| tissue culture assays using caco-2 cell line differentiate virulent from non-virulent listeria monocytogenes strains. | within the group of listeria sp., only l. monocytogenes is pathogenic for humans and numerous studies of l. monocytogenes strains have described non-virulent isolates. in this study, the potential value of two tissue culture assays (tca) was analysed to ascertain the virulence properties of l. monocytogenes strains, initially typed for virulence using the immunocompromised mouse model (icmm). the first assay assessed both the penetration into, and multiplication within, caco-2 cells (pm assay): ... | 1998 | 9750307 |
| light inactivation of food-related pathogenic bacteria using a pulsed power source. | the effects of high intensity light emissions, produced by a novel pulsed power energization technique (ppet), on the survival of bacterial populations of verocytotoxigenic escherichia coli (serotype 0157:h7) and listeria monocytogenes (serotype 4b) were investigated. using this ppet approach, many megawatts (mw) of peak electrical power were dissipated in the light source in an extremely short energization time (about 1 microsecond). the light source was subjected to electric field levels great ... | 1998 | 9750325 |
| bacterial phospholipases and intracellular growth: the two distinct phospholipases c of listeria monocytogenes. | 1998 | 9750357 | |
| [obstructive renal insufficiency caused by amoxicillin crystalluria]. | a 76-year-old woman was admitted to the icu for a meningitis with rhombencephalitis due to listeria monocytogenes. the treatment included amoxicillin (250 mg.kg-1.day-1) and gentamicin (3 mg.kg-1.day-1 over 6 days). neurological outcome was favourable. however at the 14th day, an acute renal failure occurred, following macroscopic haematuria and milkiness urine. ct scan and sonography confirmed the diagnosis of obstructive renal failure with bilateral ureteral obstruction. crystalluria caused by ... | 1997 | 9750621 |
| listeria monocytogenes-based assays for actin assembly factors. | 1998 | 9751876 | |
| efficient induction of cytotoxic cd8+ t cells against exogenous proteins: establishment and characterization of a t cell line specific for the membrane protein acta of listeria monocytogenes. | the property of listeriolysin (llo) to introduce soluble passenger proteins into the cytosol of antigen-presenting cells allows the induction of cd8+ cytotoxic t cells against such antigens. to overcome the potential problem of presentation of the immunodominant epitope ll091-99 by h-2kd, a variant llo92a was established in which tyr 92 was replaced by ala. immunization of balb/c mice with purified llo92a failed to stimulate cytotoxic t cells specific for either the epitope llo91-99 or for any o ... | 1998 | 9754551 |
| innate resistance to infection by intracellular bacterial pathogens differs in mice selected for maximal or minimal acute inflammatory response. | the intensity of nonspecific immune reaction and the host resistance to facultative intracellular pathogens are found to be associated in lines of mice selected for maximal (airmax) or minimal (airmin) acute inflammatory reactivity. airmax are more resistant than airmin mice to salmonella typhimurium and listeria monocytogenes infection, the differences between lines in ld50 being > 1000 and 100 times, respectively. this difference was shown to be related to the initial bacterial containment at ... | 1998 | 9754578 |
| effect of a traditional chinese medicine, bu-zhong-yi-qi-tang on the protection against an oral infection with listeria monocytogenes. | the protective effect against an oral infection with listeria monocytogenes was observed in balb/c mice who were orally administered a traditional chinese medicine, bu-zhong-yi-qi-tang (japanese name: hochu-ekki-to, hot) daily for 7 days. bacterial numbers in the peyer's patch (pp) at 18 h, in the mesenteric lymph nodes (mln) at 18 h, 1 day and 3 days and in the liver at 3 days after infection were significantly suppressed in hot-treated mice, although there was no difference in the bacterial nu ... | 1998 | 9754907 |
| intracellular delivery and antibacterial activity of gentamicin encapsulated in ph-sensitive liposomes. | cell membranes are relatively impermeable to the antibiotic gentamicin, a factor that, along with the toxicity of gentamicin, precludes its use against many important intracellular bacterial infections. liposomal encapsulation of this drug was used in order to achieve intracellular antibiotic delivery and therefore increase the drug's therapeutic activity against intracellular pathogens. gentamicin encapsulation in several dipalmitoylphosphatidylcholine (dppc) and ph-sensitive dioleoylphosphatid ... | 1998 | 9756749 |
| capacity of nine thermostable dna polymerases to mediate dna amplification in the presence of pcr-inhibiting samples. | the pcr is an extremely powerful method for detecting microorganisms. however, its full potential as a rapid detection method is limited by the inhibition of the thermostable dna polymerase from thermus aquaticus by many components found in complex biological samples. in this study, we have compared the effects of known pcr-inhibiting samples on nine thermostable dna polymerases. samples of blood, cheese, feces, and meat, as well as various ions, were added to pcr mixtures containing various the ... | 1998 | 9758794 |
| the combined affects of modified atmosphere, temperature, nisin and alta 2341 on the growth of listeria monocytogenes. | a cocktail of seven listeria monocytogenes isolates of food, human and environmental origin was used to assess the antilisterial activity of the bacteriocins nisin and alta 2341 in combination with various atmospheres: air, 100% n2, 40% co2:60% n2, or 100% co2. buffered tryptone soya broth (ph 6.0) was used as the growth medium and incubation was at 4 degrees c (21 days) or 12 degrees c (7 days), or when temperature fluctuated between these values for defined periods. it was observed that atmosp ... | 1998 | 9761335 |
| comparison of five typing methods for the epidemiological study of listeria monocytogenes. | five typing methods were compared in a study designed to adapt a strategy for epidemiologically typing large numbers of listeria monocytogenes strains. the methods studied were serotyping, electrophoretic typing of esterases (zymotyping), restriction fragment length polymorphism of ribosomal dna (ribotyping), random amplified polymorphic dna (rapd) and pulsed-field gel electrophoresis (pfge). data were analysed by computer-assisted statistical analysis. included in the analysis were 35 strains o ... | 1998 | 9761339 |
| crystallization and preliminary x-ray analysis of human platelet profilin complexed with an oligo proline peptide. | profilin is an actin-monomer binding protein that regulates the distribution and dynamics of the actin cytoskeleton. profilin binds poly-l-proline and proline-rich peptides in vitro and co-localizes with proline-rich proteins in focal adhesions and at the site of actin tail assembly on the surface of intracellular parasites such as listeria monocytogenes. the crystallization of the complex between human platelet profilin (hpp) and an l-proline decamer [(pro)10] is reported here. diffraction from ... | 1998 | 9761827 |
| effect of saline concentration, ph and growth temperature on the invasive capacity of listeria monocytogenes. | the invasive ability of listeria monocytogenes was monitored after treatment at different ph, temperature and salt concentrations. we found a complete loss of invasive ability in bacteria grown at ph < or = 4.5 independently of the incubation temperature (4, 22 and 30 degrees c). increasing salt concentrations at 22 and 30 degrees c had no effect at ph 7, while drastically affecting invasive ability at ph 5. the expression of two proteins of 30 and 88 kda, extracted from the culture supernatant ... | 1997 | 9765809 |
| a nested pcr method to detect listeria monocytogenes in artificially contaminated blood specimens. | a nested pcr-based test was developed for the detection of listeria monocytogenes in blood specimens from patients with listeriosis. two pairs of oligonucleotide primers were designed to amplify a 1395-bp and a 453-bp fragment of the iap gene of l. monocytogenes. amplified products were analysed with gel electrophoresis and stained with ethidium bromide. the pcr method described could be routinely used to diagnose listeriosis. | 1997 | 9765826 |
| evidence for a bacteriocin-like substance produced by a new strain of streptococcus sp., inhibitory to gram-positive food-borne pathogens. | a new strain of streptococcus sp. (cncm i-841) isolated from a commercial probiotic product was shown to be antagonistic towards several food-borne pathogens including clostridium sp. and listeria monocytogenes. this strain produced and excreted an antibacterial substance in mrs broth. the inhibitory substance was different from hydrogen peroxide, since it was unaffected by catalase. it was sensitive to proteolytic enzymes, indicating that the active moiety of the inhibitor was proteinaceous in ... | 1997 | 9765859 |
| reactivities of genus-specific monoclonal antibody em-6e11 against listeria species and serotypes of listeria monocytogenes grown in nonselective and selective enrichment broth media. | depending on the growth medium used for enrichment of bacterial cells prior to assay, the monoclonal antibody (mab) em-6e11 recognizing listeria genus-specific epitope on 43 and 94 to 97 kda cell-surface antigens (a. k. bhunia and m. g. johnson, appl. environ. microbiol. 58:1924-1929, 1992) exhibited extensive variability in the detection of listeria species. mab em-6e11 strongly detected live cells of all listeria species and all serotypes of l. monocytogenes by elisa when cells were grown in n ... | 1998 | 9766076 |
| identification of listeria monocytogenes from unpasteurized apple juice using rapid test kits. | a microbiological survey of 50 retail juices was conducted in the fall of 1996. these juices were analyzed for listeria monocytogenes, escherichia coli o157:h7, salmonella, coliforms, fecal coliforms, and ph. two unpasteurized juices were positive for l. monocytogenes: an apple juice and an apple raspberry blend with a ph of 3.78 and 3.75, respectively. three l. monocytogenes isolates were characterized. the colonies were typical for listeria sp. on oxford and lithium chloride-phenylethanol-moxa ... | 1998 | 9766077 |
| inactivation of listeria monocytogenes in milk by pulsed electric field. | pasteurized whole, 2%, and skim milk were inoculated with listeria monocytogenes scott a and treated with high-voltage pulsed electric field (pef). the effects of milk composition (fat content) and pef parameters (electric field strength, treatment time, and treatment temperature) on the inactivation of the bacterium were studied. no significant differences were observed in the inactivation of l. monocytogenes scott a in three types of milk by pef treatment. with treatment at 25 degrees c, 1- to ... | 1998 | 9766078 |
| listeria infection after liver transplantation: report of a case and review of the literature. | listeria monocytogenes is a well-recognized cause of bacteremia and meningitis in immunocompromised individuals, including recipients of solid organ transplants, but has only rarely been reported following orthotopic liver transplantation (olt). most previously reported cases of listeriosis occurred months to years following liver transplantation; we describe a case of listeriosis that occurred within 1 wk of liver transplantation, shortly after discontinuation of trimethoprim-sulfamethoxazole p ... | 1998 | 9772060 |
| effects of prolonged exposure to morphine and methadone on in vivo parameters of immune function in rats. | in rats, two 6-week repeated dose oral toxicity studies were performed with morphine (250 and 500 mg/kg food) and methadone (200 and 400 mg/kg food), respectively. alterations in immune function were studied by assessing primary and secondary immune responses to sheep red blood cells. in addition, the ability to resist challenge with infectious agents was measured in host resistance models employing the parasite trichinella spiralis and the bacterium listeria monocytogenes. the primary and secon ... | 1998 | 9772098 |
| central nervous system infection with listeria monocytogenes. 33 years' experience at a general hospital and review of 776 episodes from the literature. | we reviewed 776 previously reported and 44 new cases of cns listeriosis outside of pregnancy and the neonatal period, and evaluated the epidemiologic, diagnostic, and therapeutic characteristics of this infection. among patients with listeria meningitis/meningoencephalitis, hematologic malignancy and kidney transplantation were the leading predisposing factors, but 36% of patients had no underlying diseases recognized. the infection occurred throughout life, with a higher incidence before the ag ... | 1998 | 9772921 |
| [listeria monocytogenes: a rare cause of spontaneous bacterial peritonitis in liver cirrhosis]. | 1998 | 9775176 | |
| bone marrow cellular composition in listeria monocytogenes infected mice detected using er-mp12 and er-mp20 antibodies: a flow cytometric alternative to differential counting. | detailed assessment of bone marrow cellular composition is essential in the evaluation of various experimental in vivo systems, such as expression of transgenes, null mutations and stimulation of host defence in infection. traditional morphological analysis of mouse bone marrow is laborious, requires specific cytological expertise, and is somewhat subjective. as an alternative, we have examined whether double labelling of bone marrow with the anti-precursor monoclonal antibodies er-mp12 and er-m ... | 1998 | 9776572 |
| studying m cells and their role in infection. | in addition to sampling antigens, m cells are a common route for pathogen invasion. recent studies have partly defined the mechanisms by which pathogens interact with and exploit m cells as a gateway into the host. new research tools are facilitating studies on m cell infection, differentiation and function. | 1998 | 9778729 |
| heat-killed listeria monocytogenes as an adjuvant converts established murine th2-dominated immune responses into th1-dominated responses. | we investigated the capacity of heat-killed listeria monocytogenes (hkl), a potent stimulator of the innate immune system, as a vaccine adjuvant to modify both primary and secondary ag-specific immune responses. mice immunized with the ag keyhole limpet hemocyanin (klh) mixed with hkl generated a klh-specific primary response characterized by production of th1 cytokines and large quantities of klh-specific igg2a ab. moreover, administration of klh with hkl as an adjuvant reversed established imm ... | 1998 | 9780187 |
| use of the protective antigen of erysipelothrix rhusiopathiae in the enzyme-linked immunosorbent assay and latex agglutination. | to establish a safe and convenient serodiagnostic method for swine erysipelas, a purified protective protein antigen of erysipelothrix rhusiopathiae, which included a large amount of protective protein (64 kda protein), was used for enzyme-linked immunosorbent assay (elisa) and the latex agglutination (la) test. in the elisa, the antisera to four different serovars (1a, 2, 5 and 20) of e. rhusiopathiae exhibit a positive reaction, while antisera to other species of bacteria (listeria monocytogen ... | 1998 | 9780828 |
| [newborn infant bacterial infections. diagnosis, treatment principles, prevention]. | 1998 | 9781160 |