Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| risk for tuberculosis disease among contacts with prior positive tuberculin skin test: a retrospective cohort study, new york city. | patients with prior positive tuberculin skin test (tst) results may benefit from prophylaxis after repeat exposure to infectious tuberculosis (tb). | 2015 | 25605533 |
| keratinocyte growth factor administration attenuates murine pulmonary mycobacterium tuberculosis infection through granulocyte-macrophage colony-stimulating factor (gm-csf)-dependent macrophage activation and phagolysosome fusion. | augmentation of innate immune defenses is an appealing adjunctive strategy for treatment of pulmonary mycobacterium tuberculosis infections, especially those caused by drug-resistant strains. the effect of intranasal administration of keratinocyte growth factor (kgf), an epithelial mitogen and differentiation factor, on m. tuberculosis infection in mice was tested in prophylaxis, treatment, and rescue scenarios. infection of c57bl6 mice with m. tuberculosis resulted in inoculum size-dependent we ... | 2015 | 25605711 |
| neither primary nor memory immunity to mycobacterium tuberculosis infection is compromised in mice with chronic enteric helminth infection. | previously we had reported that nippostrongylus brasiliensis, a helminth with a lung migratory phase, affected host resistance against mycobacterium tuberculosis infection through the induction of alternatively activated (m2) macrophages. several helminth species do not have an obligatory lung migratory phase but establish chronic infections in the host that include potent immune downregulatory effects, in part mediated through induction of a foxp3(+) t regulatory cell (treg) response. treg cell ... | 2015 | 25605766 |
| genetically diverse mice are novel and valuable models of age-associated susceptibility to mycobacterium tuberculosis. | tuberculosis, the disease due to mycobacterium tuberculosis, is an important cause of morbidity and mortality in the elderly. use of mouse models may accelerate insight into the disease and tests of therapies since mice age thirty times faster than humans. however, the majority of tb research relies on inbred mouse strains, and these results might not extrapolate well to the genetically diverse human population. we report here the first tests of m. tuberculosis infection in genetically heterogen ... | 2014 | 25606048 |
| autophagy in the fight against tuberculosis. | tuberculosis (tb), a chronic infectious disease mainly caused by the tubercle bacillus mycobacterium tuberculosis, is one of the world's deadliest diseases that has afflicted humanity since ancient times. although the number of people falling ill with tb each year is declining, its incidence in many developing countries is still a major cause of concern. upon invading host cells by phagocytosis, m. tuberculosis can replicate within infected cells by arresting the maturation of the phagosome whos ... | 2015 | 25607549 |
| mazf ribonucleases promote mycobacterium tuberculosis drug tolerance and virulence in guinea pigs. | toxin-antitoxin (ta) systems are highly conserved in members of the mycobacterium tuberculosis (mtb) complex and have been proposed to play an important role in physiology and virulence. nine of these ta systems belong to the mazef family, encoding the intracellular mazf toxin and its antitoxin, maze. by overexpressing each of the nine putative mazf homologues in mycobacterium bovis bcg, here we show that rv1102c (mazf3), rv1991c (mazf6) and rv2801c (mazf9) induce bacteriostasis. the constructio ... | 2015 | 25608501 |
| surface plasmon resonator using high sensitive resonance telecommunication wavelengths for dna sensors of mycobacterium tuberculosis with thiol-modified probes. | various analytes can be verified by surface plasmon resonance, thus continuous improvement of this sensing technology is crucial for better sensing selection and higher sensitivity. the spr sensitivity on the wavelength modulation is enhanced with increasing wavelengths. the telecommunication wavelength range was then utilized to detect mycobacterium tuberculosis (mtb) deoxyribonucleic acid (dna) under two situations, without immobilization and with 5'-thiol end labeled is6100 dna probes, for sp ... | 2014 | 25609049 |
| detection of mycobacterium tuberculosis by easynat diagnostic kit in sputum samples from tanzania. | the easynat assay was evaluated for the detection of tuberculosis in sputum smears from presumptive pulmonary tuberculosis (tb) patients in an african high-tb and high-hiv setting. the sensitivity of the easynat assay was 66.7%, and the specificity and positive predictive value were 100% for the culture-positive patients. the sensitivity was only 10% in the smear-negative and culture-positive patients. | 2015 | 25609720 |
| evaluation of a low-density hydrogel microarray technique for mycobacterial species identification. | in addition to the obligatory pathogenic species of the mycobacterium tuberculosis complex and mycobacterium leprae, the genus mycobacterium also includes conditionally pathogenic species that in rare cases can lead to the development of nontuberculous mycobacterial diseases. because tuberculosis and mycobacteriosis have similar clinical signs, the accurate identification of the causative agent in a clinical microbiology laboratory is important for diagnostic verification and appropriate treatme ... | 2015 | 25609722 |
| discordance between xpert mtb/rif assay and bactec mgit 960 culture system for detection of rifampin-resistant mycobacterium tuberculosis isolates in a country with a low tuberculosis (tb) incidence. | among 452 samples that were positive by the xpert mtb/rif (xpert) assay and mgit 960 system (mgit), 440 and 10 mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. two isolates that were rifampin susceptible by the mgit system were rifampin resistant by the xpert assay. rpob sequencing identified a silent (ctg521ttg) mutation in one isolate and a missense (gac516tac) mutation in another. the detection of rifampin resistance is imperfect w ... | 2015 | 25609730 |
| comparison of the mycobacterium growth indicator tube method and the method of proportion for drug susceptibility testing of mycobacterium tuberculosis. | tuberculosis is an important public health problem in developed and, especially, developing countries. the incidence of multi-drug-resistant mycobacterium tuberculosis (mdr-tb) has increased in recent years. mycobacterial culture and susceptibility testing must be rapidly concluded for effective treatment and control of the disease. the present study evaluated the reliability of the mycobacterium growth indicator tube (mgit) method for testing the susceptibility of m. tuberculosis to four first- ... | 2014 | 25610306 |
| variability in tuberculosis granuloma t cell responses exists, but a balance of pro- and anti-inflammatory cytokines is associated with sterilization. | lung granulomas are the pathologic hallmark of tuberculosis (tb). t cells are a major cellular component of tb lung granulomas and are known to play an important role in containment of mycobacterium tuberculosis (mtb) infection. we used cynomolgus macaques, a non-human primate model that recapitulates human tb with clinically active disease, latent infection or early infection, to understand functional characteristics and dynamics of t cells in individual granulomas. we sought to correlate t cel ... | 2015 | 25611466 |
| construction of two listeria ivanovii attenuated strains expressing mycobacterium tuberculosis antigens for tb vaccine purposes. | bacillus calmette-guerin (bcg) has failed in complete control of tuberculosis (tb), thus, novel tuberculosis vaccines are urgently needed. we have constructed several tb vaccine candidates, which are characterized by the use of listeria ivanovii (li) strain as an antigen delivery vector. two l. ivanovii attenuated recombinant strains l. ivanovii△actaplcb-rv0129c and l. ivanovii△actaplcb-rv3875 were successfully screened. results from genome pcr and sequencing showed that the mycobacterium tuberc ... | 2015 | 25612873 |
| the effects of sp110's associated genes on fresh cavitary pulmonary tuberculosis in han chinese population. | sp110 is a promising anti-mycobacterium tuberculosis (mtb) gene. to investigate the effects of sp110 and its associated genes, i.e., mybbp1a and rela, on pathological progression of mtb infection, an association study with 424 patients of fresh pulmonary tuberculosis (ptb) and 424 healthy controls was performed. moreover, classification and regression tree and multifactor dimensionality reduction were employed to explore the effects of gene-gene interactions on cavitary ptb. the results indicate ... | 2016 | 25612917 |
| [tubercular spondylodiscitis in a young woman with thoracic backpain]. | we present a case of a young woman experiencing high thoracic backpain and neurological symptoms of medullar compression due to spondylodiscitis from mycobacterium tuberculosis. an important differential diagnosis of spondylodiscitis is metastatic lesions of the spine. metastatic lesions most often appear in the thoracic region of the spine and high thoracic pain should be considered a "red flag". both conditions often show up late on conventional imaging and we discuss the need for the primary ... | 2015 | 25612960 |
| [clinical differentiation between intestinal tuberculosis and morbus crohn]. | we describe a case of intestinal tuberculosis in a 34-year-old indonesian woman. she presented with diarrhoea, weight loss, fever and night sweat over a period of weeks. she underwent colonoscopy which showed a major cobblestone pattern like necrosis in part of the colon. an mr scan showed oedema involving the small intestine near the ileocoecal area and abdominal lymphadenopathy. because of the patient history of recent tb exposure, ethnicity and positive t-spot-tb, she was given a course of fo ... | 2015 | 25612982 |
| anti-mycobacterial peptides: from human to phage. | mycobacterium tuberculosis is the major pathogen of tuberculosis (tb). with the growing problem of m. tuberculosis resistant to conventional antibiotics, especially multi-drug resistant tuberculosis (mdr-tb) and extensively-drug resistant tuberculosis (xdr-tb), the need for new tb drugs is now more prominent than ever. among the promising candidates for anti-tb drugs, anti-mycobacterial peptides have a few advantages, such as low immunogenicity, selective affinity to prokaryotic negatively charg ... | 2015 | 25613372 |
| total synthesis of (-)-caprazamycin a. | caprazamycin a has significant antibacterial activity against mycobacterium tuberculosis (tb). the first total synthesis is herein reported and features a) the scalable preparation of the syn-β-hydroxy amino acid with a thiourea-catalyzed diastereoselective aldol reaction, b) construction of a diazepanone with an unstable fatty-acid side chain, and c) global deprotection with hydrogenation. this report provides a route for the synthesis of related liponucleoside antibiotics with fatty-acid side ... | 2015 | 25613457 |
| increasing the structural coverage of tuberculosis drug targets. | high-resolution three-dimensional structures of essential mycobacterium tuberculosis (mtb) proteins provide templates for tb drug design, but are available for only a small fraction of the mtb proteome. here we evaluate an intra-genus "homolog-rescue" strategy to increase the structural information available for tb drug discovery by using mycobacterial homologs with conserved active sites. of 179 potential tb drug targets selected for x-ray structure determination, only 16 yielded a crystal stru ... | 2015 | 25613812 |
| 'tree-in-bud': thinking beyond infectious causes. | high-resolution computed tomography is an important diagnostic instrument in pneumology. the 'tree-in-bud' sign is a common finding in hrct scans. the list of the most frequent differential diagnoses for 'tree-in-bud' sign includes infections with mycobacterium tuberculosis, nontuberculous mycobacteria, and other bacterial, fungal, or viral pathogens. other causes could be immunological, congenital, and idiopathic disorders as well as aspiration or inhalation of toxic agents. rare differential d ... | 2015 | 25613822 |
| novel, potent, orally bioavailable and selective mycobacterial atp synthase inhibitors that demonstrated activity against both replicating and non-replicating m. tuberculosis. | the mycobacterial f0f1-atp synthase (atpase) is a validated target for the development of tuberculosis (tb) therapeutics. therefore, a series of eighteen novel compounds has been designed, synthesized and evaluated against mycobacterium smegmatis atpase. the observed atpase inhibitory activities (ic50) of these compounds range between 0.36 and 5.45μm. the lead compound 9d [n-(7-chloro-2-methylquinolin-4-yl)-n-(3-((diethylamino)methyl)-4-hydroxyphenyl)-2,3-dichlorobenzenesulfonamide] with null cy ... | 2015 | 25614114 |
| fast and low-cost decentralized surveillance of transmission of tuberculosis based on strain-specific pcrs tailored from whole genome sequencing data: a pilot study. | molecular epidemiology has transformed our knowledge of how tuberculosis (tb) is transmitted. whole genome sequencing (wgs) has reached unprecedented levels of accuracy. however, it has increased technical requirements and costs, and analysis of data delays results. our objective was to find a way to reconcile speed and ease of implementation with the high resolution of wgs. the targeted regional allele-specific oligonucleotide pcr (trap) assay presented here is based on allele-specific pcr targ ... | 2015 | 25614157 |
| type i interferons in infectious disease. | type i interferons (ifns) have diverse effects on innate and adaptive immune cells during infection with viruses, bacteria, parasites and fungi, directly and/or indirectly through the induction of other mediators. type i ifns are important for host defence against viruses. however, recently, they have been shown to cause immunopathology in some acute viral infections, such as influenza virus infection. conversely, they can lead to immunosuppression during chronic viral infections, such as lympho ... | 2015 | 25614319 |
| copper homeostasis in mycobacterium tuberculosis. | copper (cu) is a trace element essential for the growth and development of almost all organisms, including bacteria. however, cu overload in most systems is toxic. studies show cu accumulates in macrophage phagosomes infected with bacteria, suggesting cu provides an innate immune mechanism to combat invading pathogens. to counteract the host-supplied cu, increasing evidence suggests that bacteria have evolved cu resistance mechanisms to facilitate their pathogenesis. in particular, mycobacterium ... | 2015 | 25614981 |
| clinics of ocular tuberculosis. | ocular tuberculosis is an extrapulmonary tuberculous condition and has variable manifestations. the purpose of this review is to describe the clinical manifestations of ocular tuberculosis affecting the anterior and posterior segments of the eye in both immunocompetent and immunocompromised patients. | 2015 | 25615807 |
| devr pcr for the diagnosis of intraocular tuberculosis. | to compare the efficacy of devr and mpb64 pcr in the diagnosis of intraocular tuberculosis. | 2015 | 25615810 |
| a rapid amplification/detection assay for analysis of mycobacterium tuberculosis using an isothermal and silicon bio-photonic sensor complex. | global tuberculosis (tb) control is hampered by cost and slow or insensitive diagnostic methods to be used for tb diagnosis in clinic. thus, tb still remains a major global health problem. the failure to rapidly and accurately diagnose of tb has posed significant challenges with consequent secondary resistance and ongoing transmission. we developed a rapid mycobacterium tuberculosis (mtb) amplification/detection method, called mtb isothermal solid-phase amplification/detection (mtb-isad), that c ... | 2015 | 25615836 |
| antibacterial drug discovery: doing it right. | antibacterial drug discovery needs to develop new strategies to identify attractive hit-target couples for more effective lead finding and optimization. in this issue of chemistry & biology, park and coworkers describe a novel target-based whole cell combination screening approach resulting in the identification of new inhibitors of biotin biosynthesis in mycobacterium tuberculosis. | 2015 | 25615950 |
| purification, crystallization and preliminary x-ray crystallographic studies of rv3899c from mycobacterium tuberculosis. | rv3899c, a hypothetical protein from mycobacterium tuberculosis that is conserved within the mycobacteria, is predicted to be secreted and has been found in culture filtrates. here, rv3899c has been cloned, expressed in escherichia coli and purified using standard chromatographic techniques. the hanging-drop vapour-diffusion method with peg 3350 as a precipitant was used to crystallize the protein. n-terminal sequencing results showed that the amino-acid sequence of the crystallized protein bega ... | 2015 | 25615980 |
| tlr9 gene region polymorphisms and susceptibility to tuberculosis in vietnam. | humans exposed to mycobacterium tuberculosis (mtb) show variation in susceptibility to infection and differences in tuberculosis (tb) disease outcome. toll-like receptor 9 (tlr9) is a pattern recognition receptor that mediates recognition of mtb and modulates mtb-specific t-cell responses. using a case-population design, we evaluated whether single nucleotide polymorphisms (snps) in the tlr9 gene region are associated with susceptibility to pulmonary or meningeal tb as well as neurologic present ... | 2015 | 25616954 |
| correction for das et al., structure of crga, a cell division structural and regulatory protein from mycobacterium tuberculosis, in lipid bilayers. | 2015 | 25617366 | |
| erratum for jamieson et al., whole-genome sequencing of the mycobacterium tuberculosis manila sublineage results in less clustering and better resolution than mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (miru-vntr) typing and spoligotyping. | 2015 | 25617443 | |
| monocarbonyl analogs of curcumin inhibit growth of antibiotic sensitive and resistant strains of mycobacterium tuberculosis. | tuberculosis (tb) is a major public health concern worldwide with over 2 billion people currently infected. the rise of strains of mycobacterium tuberculosis (mtb) that are resistant to some or all first and second line antibiotics, including multidrug-resistant (mdr), extensively drug resistant (xdr) and totally drug resistant (tdr) strains, is of particular concern and new anti-tb drugs are urgently needed. curcumin, a natural product used in traditional medicine in india, exhibits anti-microb ... | 2015 | 25618016 |
| the first small-molecule inhibitors of members of the ribonuclease e family. | the escherichia coli endoribonuclease rnase e is central to the processing and degradation of all types of rna and as such is a pleotropic regulator of gene expression. it is essential for growth and was one of the first examples of an endonuclease that can recognise the 5'-monophosphorylated ends of rna thereby increasing the efficiency of many cleavages. homologues of rnase e can be found in many bacterial families including important pathogens, but no homologues have been identified in humans ... | 2015 | 25619596 |
| stressed mycobacteria use the chaperone clpb to sequester irreversibly oxidized proteins asymmetrically within and between cells. | mycobacterium tuberculosis (mtb) defends itself against host immunity and chemotherapy at several levels, including the repair or degradation of irreversibly oxidized proteins (iops). to investigate how mtb deals with iops that can neither be repaired nor degraded, we used new chemical and biochemical probes and improved image analysis algorithms for time-lapse microscopy to reveal a defense against stationary phase stress, oxidants, and antibiotics--the sequestration of iops into aggregates in ... | 2015 | 25620549 |
| whole genome response in guinea pigs infected with the high virulence strain mycobacterium tuberculosis tt372. | in this study we conducted a microarray-based whole genomic analysis of gene expression in the lungs after exposure of guinea pigs to a low dose aerosol of the atypical beijing western cape tt372 strain of mycobacterium tuberculosis, after harvesting lung tissues three weeks after infection at a time that effector immunity is starting to peak. the infection resulted in a very large up-regulation of multiple genes at this time, particularly in the context of a "chemokine storm" in the lungs. over ... | 2014 | 25621360 |
| enhancement of in vitro activity of tuberculosis drugs by addition of thioridazine is not reflected by improved in vivo therapeutic efficacy. | assessment of the activity of thioridazine towards mycobacterium tuberculosis (mtb), in vitro and in vivo as a single drug and in combination with tuberculosis (tb) drugs. | 2014 | 25621361 |
| bactericidal activity of pyrazinamide and clofazimine alone and in combinations with pretomanid and bedaquiline. | new regimens to shorten tuberculosis treatment and manage patients with drug-resistant tuberculosis who are infected with hiv are urgently needed. experimental and clinical evidence suggests that the new drugs bedaquiline (b) and pretomanid (pa), combined with an existing drug, pyrazinamide (z), and a repurposed drug, clofazimine (c), may assist treatment shortening of drug-susceptible and drug-resistant tuberculosis. | 2015 | 25622149 |
| structure-function analysis of vapb4 antitoxin identifies critical features of a minimal vapc4 toxin-binding module. | bacterial toxin-antitoxin systems play a critical role in the regulation of gene expression, leading to developmental changes, reversible dormancy, and cell death. type ii toxin-antitoxin pairs, composed of protein toxins and antitoxins, exist in nearly all bacteria and are classified into six groups on the basis of the structure of the toxins. the vapbc group comprises the most common type ii system and, like other toxin-antitoxin systems, functions to elicit dormancy by inhibiting protein synt ... | 2015 | 25622615 |
| latency-associated protein rv2660c of mycobacterium tuberculosis augments expression of proinflammatory cytokines in human macrophages by interacting with tlr2. | one-third of the world's human population is latently infected with mycobacterium tuberculosis (mtb), and individuals with latent infection have a 10% risk of developing active tuberculosis (tb) disease in their lifetime. rv2660c (encoding a hypothetical protein) is closely correlated with dormancy of mtb. studies have found that rv2660c was preferentially expressed during latent infection for adaptation to lack of nutrition and hypoxia; however, the precise function is unknown. identification a ... | 2015 | 25622945 |
| pilot study to detect airborne mycobacterium tuberculosis exposure in a south african public healthcare facility outpatient clinic. | airborne transmission of mycobacterium tuberculosis remains an occupational health hazard, particularly in crowded and resource-limited healthcare settings. | 2015 | 25623206 |
| drug-resistant tuberculosis in hiv-infected patients in a national referral hospital, phnom penh, cambodia. | there are no recent data on the prevalence of drug-resistant tuberculosis (dr tb) in cambodia. we aim to describe tb drug resistance amongst adults with pulmonary and extra-pulmonary tb and human immunodeficiency virus (hiv) co-infection in a national referral hospital in phnom penh, cambodia. | 2015 | 25623609 |
| comparison of the clinical characteristics of mycobacterium tuberculosis and nontuberculous mycobacteria patients with joint involvement. | nontuberculous mycobacteria (ntm) joint involvement is rare. however, the incidence of ntm disease is increasing and it is difficult to distinguish ntm from mycobacterium tuberculosis (mtb). here, the clinical characteristics of ntm joint involvement were compared with those of mtb. distal joint involvement and precipitating factors were significantly more frequent for ntm joint infections. because pathologic findings of ntm and mtb were similar, microbiological investigations are needed. | 2015 | 25623639 |
| the proprotein convertase subtilisin/kexin furina regulates zebrafish host response against mycobacterium marinum. | tuberculosis is a chronic bacterial disease with a complex pathogenesis. an effective immunity against mycobacterium tuberculosis requires both the innate and adaptive immune responses, including proper t helper (th) type 1 cell function. furin is a proprotein convertase subtilisin/kexin (pcsk) enzyme, which is highly expressed in th1 type cells. furin expression in t cells is essential for maintaining peripheral immune tolerance, but its role in the innate immunity and infections has remained e ... | 2015 | 25624351 |
| anti-vascular endothelial growth factor treatment normalizes tuberculosis granuloma vasculature and improves small molecule delivery. | tuberculosis (tb) causes almost 2 million deaths annually, and an increasing number of patients are resistant to existing therapies. patients who have tb require lengthy chemotherapy, possibly because of poor penetration of antibiotics into granulomas where the bacilli reside. granulomas are morphologically similar to solid cancerous tumors in that they contain hypoxic microenvironments and can be highly fibrotic. here, we show that tb-infected rabbits have impaired small molecule distribution i ... | 2015 | 25624495 |
| severe mycobacterium tuberculosis-related immune reconstitution syndrome in an immunocompetent patient. | we present a young immunocompetent male with diagnosed sputum culture-positive tuberculosis on intensive phase with observed daily four-drug antituberculosis therapy. he presented at 1-month of treatment with sequential bilateral pneumothoraces, increase in cavitation and consolidation and respiratory failure. repeat smears for acid-fast bacilli had downgraded, and cultures were negative. quantiferon-gold (initially negative) was now strongly positive. a diagnosis of possible immune reconstituti ... | 2015 | 25624652 |
| mobile based gold nanoprobe tb diagnostics for point-of-need. | nanotechnology based diagnostics has provided improved tools for pathogen detection and sensitive and specific characterization of antibiotic resistance signatures. tuberculosis (tb) is caused by members of the mycobacterium tuberculosis complex (mtbc) and, according to the world health organization, is one of the most serious infectious diseases in the world. recent advances in molecular diagnostics of tb have improved both the detection time and sensitivity but they still require specialized t ... | 2015 | 25626530 |
| transcription and translation of the rpsj, rpln and rrna operons of the tubercle bacillus. | several species of the genus mycobacterium are human pathogens, notably the tubercle bacillus (mycobacterium tuberculosis). the rate of proliferation of a bacterium is reflected in the rate of ribosome synthesis. this report describes a quantitative analysis of the early stages of the synthesis of ribosomes of m. tuberculosis. specifically, the roles of three large operons, namely: the rrn operon (1.7 microns) encoding rrs (16s rrna), rrl (23s rrna) and rrf (5s rrna); the rpsj operon (1.93 micro ... | 2015 | 25627442 |
| immune requirements for protective th17 recall responses to mycobacterium tuberculosis challenge. | tuberculosis (tb) vaccine development has focused largely on targeting t helper type 1 (th1) cells. however, despite inducing th1 cells, the recombinant tb vaccine mva85a failed to enhance protection against tb disease in humans. in recent years, th17 cells have emerged as key players in vaccine-induced protection against tb. however, the exact cytokine and immune requirements that enable th17-induced recall protection remain unclear. in this study, we have investigated the requirements for th17 ... | 2015 | 25627812 |
| primary/local hepatic tuberculosis without dissemination. | we present a rare case of primary hepatic tuberculosis in a 50-year-old man who presented with pain at the right hypochondrium. the diagnosis was established by fine-needle aspiration cytology (fnac) of the primary hepatic lesions in both lobes of the liver, which was further supported by histopathological examination and tissue pcr for mycobacterium tuberculosis in the fnac specimens. | 2015 | 25628318 |
| immune status and serial quantiferon-tb gold in-tube screening for latent mycobacterium tuberculosis infection among hiv-infected persons in a country with a low tuberculosis incidence. | 2015 | 25628342 | |
| manipulation of the endocytic pathway and phagocyte functions by mycobacterium tuberculosis lipoarabinomannan. | lipoarabinomannan is a major immunomodulatory lipoglycan found in the cell envelope of mycobacterium tuberculosis and related human pathogens. it reproduces several salient properties of m. tuberculosis in phagocytic cells, including inhibition of pro-inflammatory cytokine production, inhibition of phagolysosome biogenesis, and inhibition of apoptosis as well as autophagy. in this review, we present our current knowledge on lipoarabinomannan structure and ability to manipulate the endocytic path ... | 2014 | 25629008 |
| structome analysis of virulent mycobacterium tuberculosis, which survives with only 700 ribosomes per 0.1 fl of cytoplasm. | we previously reported the exquisite preservation of the ultrastructures of virulent mycobacterium tuberculosis cells processed through cryofixation and rapid freeze substitution. here, we report the "structome" analysis (i.e., the quantitative three-dimensional structural analysis of a whole cell at the electron microscopic level) of virulent m. tuberculosis using serial ultrathin sections prepared after cryofixation and rapid freeze substitution and analyzed by transmission electron microscopy ... | 2015 | 25629354 |
| molecular epidemiology of tuberculosis in kaohsiung city located at southern taiwan, 2000-2008. | we present the first comprehensive analysis of mycobacterium tuberculosis (mtb) isolates circulating in southern taiwan. in this 9-year population-based study, the tb situation in the kaohsiung region was characterized by genotypic analysis of 421 mtb isolates. | 2015 | 25629610 |
| development of an esi-lc-ms-based assay for kinetic evaluation of mycobacterium tuberculosis shikimate kinase activity and inhibition. | a simple and reliable liquid chromatography-mass spectrometry (lc-ms) assay has been developed and validated for the kinetic characterization and evaluation of inhibitors of shikimate kinase from mycobacterium tuberculosis (mtsk), a potential target for the development of novel antitubercular drugs. this assay is based on the direct determination of the reaction product shikimate-3-phosphate (s3p) using electrospray ionization (esi) and a quadrupole time-of-flight (q-tof) detector. a comparative ... | 2015 | 25629762 |
| head and neck tuberculosis: 6-year retrospective study. | pulmonary involvement exists in 80% of cases of infection with mycobacterium tuberculosis; however, in up to 20% of cases there may be extra-pulmonary involvement. in the ent area, the most common site is cervical lymphadenitis, affecting approximately 95% of cases. | 2016 | 25630666 |
| mycobacterial nicotinate mononucleotide adenylyltransferase: structure, mechanism, and implications for drug discovery. | nicotinate mononucleotide adenylyltransferase nadd is an essential enzyme in the biosynthesis of the nad cofactor, which has been implicated as a target for developing new antimycobacterial therapies. here we report the crystal structure of mycobacterium tuberculosis nadd (mtnadd) at a resolution of 2.4 å. a remarkable new feature of the mtnadd structure, compared with other members of this enzyme family, is a 310 helix that locks the active site in an over-closed conformation. as a result, mtna ... | 2015 | 25631047 |
| tuberculosis in australia: bacteriologically-confirmed cases and drug resistance, 2011. a report of the australian mycobacterium reference laboratory network. | the australian mycobacterium reference laboratory network collects and analyses laboratory data on new cases of disease caused by the mycobacterium tuberculosis complex. in 2011, a total of 1,057 cases were identified bacteriologically; an annual reporting rate of 4.6 cases per 100,000 population. eighteen children aged less than 15 years plus an additional 11 children from the torres strait protected zone had bacteriologically-confirmed tuberculosis. results of in vitro drug susceptibility test ... | 2014 | 25631600 |
| mycobacterial dna extraction for whole-genome sequencing from early positive liquid (mgit) cultures. | we developed a low-cost and reliable method of dna extraction from as little as 1 ml of early positive mycobacterial growth indicator tube (mgit) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical samples. the dna extraction method is based on ethanol precipitation supplemented by pretreatment steps with a molysis kit or saline wash for the removal of human dna and a final dna cleanup step with solid-phase reversi ... | 2015 | 25631807 |
| human cd8 t lymphocytes recognize mycobacterium tuberculosis antigens presented by hla-e during active tuberculosis and express type 2 cytokines. | cd8 t cells contribute to protective immunity against mycobacterium tuberculosis. in humans, m. tuberculosis reactive cd8 t cells typically recognize peptides associated to classical mhc class ia molecules, but little information is available on cd8 t cells recognizing m. tuberculosis ags presented by nonclassical mhc class ib molecules. we show here that cd8 t cells from tuberculosis (tb) patients recognize hla-e-binding m. tuberculosis peptides in a cd3/tcr αβ mediated and cd8-dependent manner ... | 2015 | 25631937 |
| prenatal passive transfer of mycobacterium tuberculosis antibodies in asian elephant (elephas maximus) calves. | asian elephant (elephas maximus) dams and their newborn calves were tested for mycobacterium tuberculosis antibodies in serum. blood was drawn from dams prior to calving and from calves on their day of birth. all six calves born to tuberculosis-reactive dams were also tuberculosis reactive, suggesting prenatal passive placental transfer of tuberculosis antibodies. in contrast, all three calves born to tuberculosis-nonreactive dams lacked detectable tuberculosis antibodies in pre-suckling or day- ... | 2014 | 25632691 |
| tuberculosis in elephants-a reemergent disease: diagnostic dilemmas, the natural history of infection, and new immunological tools. | tuberculosis (tb) in elephants has been described since ancient times. however, it was not until 1996 when infection with mycobacterium tuberculosis was identified in a herd of circus elephants that significant research into this disease began. the epidemiology and natural history of tb were unknown in elephants since there had been no comprehensive screening programs, and diagnostic techniques developed for cervidae and bovidae were of unknown value. and, while precepts of test and slaughter we ... | 2015 | 25633896 |
| the tuberculosis drug discovery and development pipeline and emerging drug targets. | the recent accelerated approval for use in extensively drug-resistant and multidrug-resistant-tuberculosis (mdr-tb) of two first-in-class tb drugs, bedaquiline and delamanid, has reinvigorated the tb drug discovery and development field. however, although several promising clinical development programs are ongoing to evaluate new tb drugs and regimens, the number of novel series represented is few. the global early-development pipeline is also woefully thin. to have a chance of achieving the goa ... | 2015 | 25635061 |
| mycobacterium tuberculosis h37rv has a single nucleotide polymorphism in phor which affects cell wall hydrophobicity and gene expression. | mycobacterium tuberculosis is a successful pathogen that can adapt to multiple environmental niches. as part of its repertoire of adaptive responses, two-component regulatory systems play a major role in co-ordinating gene expression at the global level. the phopr system controls major cellular functions, including respiration, lipid metabolism, the immediate and enduring hypoxic responses, stress responses and persistence. we identified a single nucleotide polymorphism (snp) found in the sensor ... | 2015 | 25635271 |
| a virtual screen discovers novel, fragment-sized inhibitors of mycobacterium tuberculosis inha. | isoniazid (inh) is usually administered to treat latent mycobacterium tuberculosis (mtb) infections and is used in combination therapy to treat active tuberculosis (tb). unfortunately, resistance to this drug is hampering its clinical effectiveness. inh is a prodrug that must be activated by mtb catalase-peroxidase (katg) before it can inhibit inha (mtb enoyl-acyl-carrier-protein reductase). isoniazid-resistant cases of tb found in clinical settings usually involve mutations in or deletion of ka ... | 2015 | 25636146 |
| iron acquisition strategies in mycobacteria. | iron is an essential element to most life forms including mycobacterial species. however, in the oxidative atmosphere iron exists as insoluble salts. free and soluble iron ions are scarce in both the extracellular and intracellular environment which makes iron assimilation very challenging to mycobacteria. tuberculosis, caused by the pathogen, mycobacterium tuberculosis, is one of the most infectious and deadly diseases in the world. extensive studies regarding iron acquisition strategies have b ... | 2015 | 25636179 |
| identifying essential genes in mycobacterium tuberculosis by global phenotypic profiling. | transposon sequencing (tnseq) is a next-generation deep sequencing-based method to quantitatively assess the composition of complex mutant transposon libraries after pressure from selection. although this method can be used for any organism in which transposon mutagenesis is possible, this chapter describes its use in mycobacterium tuberculosis. more specifically, the methods for generating complex libraries through transposon mutagenesis, design of selective pressure, extraction of genomic dna, ... | 2015 | 25636614 |
| interferon γ and tumor necrosis factor are not essential parameters of cd4+ t-cell responses for vaccine control of tuberculosis. | mycobacterium tuberculosis infects one third of the world's population and causes >8 million cases of tuberculosis annually. new vaccines are necessary to control the spread of tuberculosis. t cells, interferon γ (ifn-γ), and tumor necrosis factor (tnf) are necessary to control m. tuberculosis infection in both humans and unvaccinated experimental animal models. however, the immune responses necessary for vaccine efficacy against m. tuberculosis have not been defined. the multifunctional activit ... | 2015 | 25637347 |
| virtual screening for the identification of novel inhibitors of mycobacterium tuberculosis cell wall synthesis: inhibitors targeting rmlb and rmlc. | tuberculosis remains one of the deadliest infectious diseases in humans. it has caused more than 100 million deaths since its discovery in 1882. currently, more than 5 million people are infected with tb bacterium each year. the cell wall of mycobacterium tuberculosis plays an important role in maintaining the ability of mycobacteria to survive in a hostile environment. therefore, we report a virtual screening (vs) study aiming to identify novel inhibitors that simultaneously target rmlb and rml ... | 2015 | 25637777 |
| antitubercular activity of ru (ii) isoniazid complexes. | despite the resistance developed by the mycobacterium tuberculosis (mtb) strains, isoniazid (inh) has been recognized as one of the best drug for treatment of tuberculosis (tb). the coordination of inh to ruthenium metal centers was investigated as a strategy to enhance the activity of this drug against the sensitive and resistant strains of mtb. the complexes trans-[ru(nh3)4(l)(inh)](2+) (l=so2 or nh3) were isolated and their chemical and antituberculosis properties studied. the minimal inhibit ... | 2015 | 25638418 |
| mycobacterium tuberculosis reca is indispensable for inhibition of the mitogen-activated protein kinase-dependent bactericidal activity of thp-1-derived macrophages in vitro. | our knowledge about the mechanisms utilized by mycobacterium tuberculosis to survive inside macrophages is still incomplete. one of the mechanism that protects m. tuberculosis from the host's microbicidal products and allows bacteria to survive involves dna repair systems such as the homologous recombination (hr) and nonhomologous end-joining (nhej) pathways. it is accepted that any pathway that contributes to genome maintenance should be considered as potentially important virulence factor. in ... | 2015 | 25639683 |
| reduction of mycobacterium tuberculosis infection in bacillus calmette guerin immunized people is due to training of innate immunity. | the currently used vaccine for prevention of tuberculosis is bacillus calmette guerin, which has been associated with a protective effect of 51% against tuberculosis. new vaccination strategies based on an enhancement of adaptive t-cell based immunity have been unsuccessful in increasing the efficiency of bcg immunisation. the proposed hypothesis is that a reduction of mycobacterium (m.) tuberculosis infection in bacillus calmette guerin immunized people is due to training of innate immunity. ev ... | 2015 | 25639709 |
| anti-mycobacterial activity of tamoxifen against drug-resistant and intra-macrophage mycobacterium tuberculosis. | recently, it has become a struggle to treat tuberculosis with the current commercial antituberculosis drugs because of the increasing emergence of multidrug-resistant (mdr) tuberculosis and extensively drug-resistant (xdr) tuberculosis. we evaluated here the antimycobacterial activity of tamoxifen, known as a synthetic anti-estrogen, against eight drug-sensitive or resistant strains of mycobacterium tuberculosis (tb), and the active intracellular killing of tamoxifen on tb in macrophages. the re ... | 2015 | 25639719 |
| little difference between minimum inhibitory concentrations of mycobacterium tuberculosis wild-type organisms determined with bactec mgit 960 and middlebrook 7h10. | the mic wild-type (wt) distribution for mycobacterium tuberculosis in bactec 960 mgit is not defined, which may result in poor reproducibility for drug susceptibility testing (dst), as several dst methods with different breakpoints are in use. in a comparison between mgit and middlebrook 7h10 medium of seven first- and second-line drugs, including 133 mic determinations of 15 wt isolates, we found an agreement of 91.7% within ± one mic dilution step. the results confirm the agreement in mic test ... | 2015 | 25640156 |
| mycobacterium tuberculosis espb protein suppresses interferon-γ-induced autophagy in murine macrophages. | mycobacterium tuberculosis (mtb) persists within immature phagosomes by preventing their maturation into phagolysosomes. although the early secretory antigenic target 6 (esat-6) system 1 (esx-1) secretion-associated protein b (espb) of mtb is strongly linked to immunogenicity and virulence of this organism, its mechanism of action remains largely unclear. this study aimed to investigate espb effects on autophagy in murine ana-1 macrophage cells. | 2016 | 25641595 |
| mycobacterium tuberculosis suppresses innate immunity by coopting the host ubiquitin system. | mycobacterium tuberculosis ptpa, a secreted tyrosine phosphatase essential for tuberculosis pathogenicity, could be an ideal target for a drug against tuberculosis, but its active-site inhibitors lack selectivity over human phosphatases. here we found that ptpa suppressed innate immunity dependent on pathways of the kinases jnk and p38 and the transcription factor nf-κb by exploiting host ubiquitin. binding of ptpa to ubiquitin via a region with no homology to human proteins activated it to deph ... | 2015 | 25642820 |
| prisons as reservoir for community transmission of tuberculosis, brazil. | we conducted a population-based study of tuberculosis (tb) cases in dourados, brazil, to assess the relationship between incarceration and tb in the general population. incarceration was associated with tb in an urban population; 54% of mycobacterium tuberculosis strains were related to strains from persons in prisons. tb control in prisons is critical for reducing disease prevalence. | 2015 | 25642998 |
| a new butenolide cinnamate and other biological active chemical constituents from polygonum glabrum. | phytochemical investigation of the methanol extract of the aerial parts of polygonum glabrum afforded one new natural product (-)-2-methoxy-2-butenolide-3-cinnamate (1) along with six known compounds, β-hydroxyfriedalanol (2), 3-hydroxy-5-methoxystilbene (3), (-) pinocembrin (4), sitosterol-(6'-o-palmitoyl)-3-o-β-d-glucopyranoside (5), (-) pinocembrin-5-methyl ether (6) and sitosterol-3-o-β-d-glucopyranoside (7). compound 1 showed promising in vitro anti-hiv-1 activity against primary isolates h ... | 2015 | 25643642 |
| triazole: a promising antitubercular agent. | tuberculosis is a contagious disease with comparatively high mortality worldwide. the statistics shows that around three million people throughout the world die annually from tuberculosis and there are around eight million new cases each year, of which developing countries showed major share. therefore, the discovery and development of effective antituberculosis drugs with novel mechanism of action have become an insistent task for infectious diseases research programs. the literature reveals th ... | 2015 | 25643871 |
| molecular insights into the binding of coenzyme f420 to the conserved protein rv1155 from mycobacterium tuberculosis. | coenzyme f420 is a deazaflavin hydride carrier with a lower reduction potential than most flavins. in mycobacterium tuberculosis (mtb), f420 plays an important role in activating pa-824, an antituberculosis drug currently used in clinical trials. although f420 is important to mtb redox metabolism, little is known about the enzymes that bind f420 and the reactions that they catalyze. we have identified a novel f420 -binding protein, rv1155, which is annotated in the mtb genome sequence as a putat ... | 2015 | 25644473 |
| lspa gene of mycobacterium tuberculosis co-transcribes with rv1540 and induced by surface and acidic stress. | lipoprotein signal peptidase, lspa (rv1539), is the only known gene in mycobacterial genome for cleaving the signal sequence from prolipoprotein to form mature lipoprotein. it has been implicated in maintaining the virulence of mycobacterium tuberculosis. the regulation of lspa had not been studied so far. here, we identify a novel operon lspa-rv1540 in m. tuberculosis. we detected co-transcription of the open reading frames of lspa-rv1540 in in-vitro as well as in ex-vivo conditions. analysis o ... | 2015 | 25644771 |
| a clinicopathological cohort study of liver pathology in 301 patients with human immunodeficiency virus/acquired immune deficiency syndrome. | liver disease complicates human immunodeficiency virus (hiv)/acquired immune deficiency syndrome; however, liver pathology data are limited, particularly from high hiv prevalence countries. we investigated the spectrum and clinicopathological correlates of liver pathology in a high hiv burden setting. in a single-center study, all hiv/acquired immune deficiency syndrome patients with complete clinical and demographic data who underwent liver biopsy were analyzed and clinicopathologically assesse ... | 2015 | 25644940 |
| delamanid (opc-67683) for treatment of multi-drug-resistant tuberculosis. | the research and development of delamanid was carried out by otsuka pharmaceutical development and commercialization (osaka, tokyo, japan). it belongs to the group of nitroimidazoles. it inhibits the synthesis of mycolic acids, crucial component of the cell wall of the mycobacterium tuberculosis complex. it is insoluble in water and its activity was proven in several in vitro and in vivo studies. its market approval was obtained in april 2014 in europe. its bactericidal activity was demonstrated ... | 2015 | 25645397 |
| characterization of novel acyl coenzyme a dehydrogenases involved in bacterial steroid degradation. | the acyl coenzyme a (acyl-coa) dehydrogenases (acads) fade34 and casc, encoded by the cholesterol and cholate gene clusters of mycobacterium tuberculosis and rhodococcus jostii rha1, respectively, were successfully purified. both enzymes differ from previously characterized acads in that they contain two fused acyl-coa dehydrogenase domains in a single polypeptide. site-specific mutagenesis showed that only the c-terminal acad domain contains the catalytic glutamate base required for enzyme acti ... | 2015 | 25645564 |
| a novel antimycobacterial compound acts as an intracellular iron chelator. | efficient iron acquisition is crucial for the pathogenesis of mycobacterium tuberculosis. mycobacterial iron uptake and metabolism are therefore attractive targets for antitubercular drug development. resistance mutations against a novel pyrazolopyrimidinone compound (pzp) that is active against m. tuberculosis have been identified within the gene cluster encoding the esx-3 type vii secretion system. esx-3 is required for mycobacterial iron acquisition through the mycobactin siderophore pathway, ... | 2015 | 25645825 |
| the mycobacterium tuberculosis outer membrane channel protein cpnt confers susceptibility to toxic molecules. | mycobacterium tuberculosis, the causative agent of tuberculosis, is protected from toxic solutes by an effective outer membrane permeability barrier. recently, we showed that the outer membrane channel protein cpnt is required for efficient nutrient uptake by m. tuberculosis and mycobacterium bovis bcg. in this study, we found that the cpnt mutant of m. bovis bcg is more resistant than the wild type to a large number of drugs and antibiotics, including rifampin, ethambutol, clarithromycin, tetra ... | 2015 | 25645841 |
| tuberculous spondylitis in russia and prominent role of multidrug-resistant clone mycobacterium tuberculosis beijing b0/w148. | extrapulmonary and, in particular, spinal tuberculosis (tb) constitutes a minor but significant part of the total tb incidence. in spite of this, almost no studies on the genetic diversity and drug resistance of mycobacterium tuberculosis isolates from spinal tb patients have been published to date. here, we report results of the first russian and globally largest molecular study of m. tuberculosis isolates recovered from patients with tuberculous spondylitis (tbs). the majority of 107 isolates ... | 2015 | 25645851 |
| the uptake of trehalose glycolipids by macrophages is independent of mincle. | trehalose glycolipids play an important role in the pathogenesis of mycobacterium tuberculosis and are used as adjuvants for vaccines; however, much still remains unanswered about the mechanisms through which these glycolipids exert their immunomodulatory potential. recently, the macrophage-inducible c-type lectin mincle was determined to be the receptor for trehalose glycolipids, yet the role played by mincle in glycolipid uptake is unknown. accordingly, we developed several fluorescent trehalo ... | 2015 | 25645884 |
| characterization of mycobacterium tuberculosis esxa membrane insertion: roles of n- and c-terminal flexible arms and central helix-turn-helix motif. | esxa (esat-6), an important virulence factor of mycobacterium tuberculosis, plays an essential role in phagosome rupture and bacterial cytosolic translocation within host macrophages. our previous study showed that esxa exhibits a unique membrane-interacting activity that is not found in its ortholog from nonpathogenic mycobacterium smegmatis. however, the molecular mechanism of esxa membrane insertion remains unknown. in this study, we generated truncated esxa proteins with deletions of the n- ... | 2015 | 25645924 |
| the pleiotropic transcriptional response of mycobacterium tuberculosis to vitamin c is robust and overlaps with the bacterial response to multiple intracellular stresses. | mycobacterium tuberculosis (mtb) owes its success as a pathogen in large measure to its ability to exist in a persistent state of 'dormancy' resulting in a lifelong latent tuberculosis (tb) infection. an understanding of bacterial adaptation during dormancy will help in devising approaches to counter latent tb infection. in vitro models have provided valuable insights into bacterial adaptation; however, they have limitations because they do not disclose the bacterial response to the intracellula ... | 2015 | 25645949 |
| highly reproducible absolute quantification of mycobacterium tuberculosis complex by digital pcr. | digital pcr (dpcr) offers absolute quantification through the limiting dilution of template nucleic acid molecules and has the potential to offer high reproducibility. however, the robustness of dpcr has yet to be evaluated using complex genomes to compare different dpcr methods and platforms. we used dna templates from the pathogen mycobacterium tuberculosis to evaluate the impact of template type, master mixes, primer pairs and, crucially, extraction methods on dpcr performance. performance wa ... | 2015 | 25646934 |
| pleural fluid adenosine deaminase (pfada) in the diagnosis of tuberculous effusions in a low incidence population. | previous studies have assessed the diagnostic ability of pleural fluid adenosine deaminase (pfada) in detecting tuberculous pleural effusions, with good specificity and sensitivity reported. however, in north western europe pfada is not routinely used in the investigation of a patient with an undiagnosed pleural effusion, mainly due to a lack of evidence as to its utility in populations with low mycobacterium tuberculosis (mtb) incidence. | 2015 | 25647479 |
| tuberculosis as a cause or comorbidity of childhood pneumonia in tuberculosis-endemic areas: a systematic review. | pneumonia is a major cause of morbidity and mortality in infants and children worldwide, with most cases occurring in tuberculosis-endemic settings. studies have emphasised the potential importance of mycobacterium tuberculosis in acute severe pneumonia in children as a primary cause or underlying comorbidity, further emphasised by the changing aetiological range with rollout of bacterial conjugate vaccines in high mortality settings. we systematically reviewed clinical and autopsy studies done ... | 2015 | 25648115 |
| association between intrinsic disorder and serine/threonine phosphorylation in mycobacterium tuberculosis. | serine/threonine phosphorylation is an important mechanism that is involved in the regulation of protein function. in eukaryotes, phosphorylation occurs predominantly in intrinsically disordered regions of proteins. though serine/threonine phosphorylation and protein disorder are much less prevalent in prokaryotes, some bacteria have high levels of serine/threonine phosphorylation and disorder, including the medically important m. tuberculosis. here i show that serine/threonine phosphorylation s ... | 2015 | 25648268 |
| human mesenchymal stem cells: new sojourn of bacterial pathogens. | tuberculosis (tb), caused by mycobacterium tuberculosis (m. tuberculosis), is the leading infectious disease which claims one human life every 15-20s globally. the persistence of this deadly disease in human population can be attributed to the ability of the bacterium to stay in latent form. m. tuberculosis possesses a plethora of mechanisms not only to survive latently under harsh conditions inside the host but also modulate the host immune cells in its favour. various m. tuberculosis gene fami ... | 2015 | 25648374 |
| comprehensive insights into transcriptional adaptation of intracellular mycobacteria by microbe-enriched dual rna sequencing. | the human pathogen mycobacterium tuberculosis has the capacity to escape eradication by professional phagocytes. during infection, m. tuberculosis resists the harsh environment of phagosomes and actively manipulates macrophages and dendritic cells to ensure prolonged intracellular survival. in contrast to other intracellular pathogens, it has remained difficult to capture the transcriptome of mycobacteria during infection due to an unfavorable host-to-pathogen ratio. | 2015 | 25649146 |
| potential inhibitors for isocitrate lyase of mycobacterium tuberculosis and non-m. tuberculosis: a summary. | isocitrate lyase (icl) is the first enzyme involved in glyoxylate cycle. many plants and microorganisms are relying on glyoxylate cycle enzymes to survive upon downregulation of tricarboxylic acid cycle (tca cycle), especially mycobacterium tuberculosis (mtb). in fact, icl is a potential drug target for mtb in dormancy. with the urge for new antitubercular drug to overcome tuberculosis treat such as multidrug resistant strain and hiv-coinfection, the pace of drug discovery has to be increased. t ... | 2015 | 25649791 |
| cleavage of the moax-encoded fused molybdopterin synthase from mycobacterium tuberculosis is necessary for activity. | molybdopterin cofactor (moco) biosynthesis in mycobacterium tuberculosis is associated with a multiplicity of genes encoding several enzymes in the pathway, including the molybdopterin (mpt) synthase, a hetero tetramer comprising two moad and two moae subunits. in addition to moad1, moad2, moae1, moae2, the m. tuberculosis genome also contains a moax gene which encodes an mpt-synthase in which the moad and moae domains are located on a single polypeptide. in this study, we assessed the requireme ... | 2015 | 25651977 |
| synchronous oral paracoccidioidomycosis and pulmonary tuberculosis in an immunocompetent patient. | paracoccidioidomycosis (pcm) and tuberculosis (tb) are chronic granulomatous infectious diseases, in which the main form of contraction is through inhalation of the microorganism-paracoccidioides brasiliensis and mycobacterium tuberculosis. oral involvement of pcm is observed in up to 70 % of the cases and usually presents clinically as ulcerations with granular surface showing tiny hemorrhagic areas. oral presentation of tb is rare with prevalence smaller than 0.5 % of all cases. clinical prese ... | 2015 | 25652419 |
| mycobacterium tuberculosis dormancy-associated antigen of rv2660c induces stronger immune response in latent mycobacterium tuberculosis infection than that in active tuberculosis in a chinese population. | one-third of the world's human population is latently infected with mycobacterium tuberculosis (m. tb), and adult tuberculosis (tb) is largely caused by the resuscitation of latent m. tb. rv2660c, an m. tb dormancy-related antigen, is preferentially expressed during latent infection. identification and characterization of rv2660c are crucial to understanding host-pathogen interactions and to develop drug target and vaccine candidates. in this study, t-cell and antibody immune responses against r ... | 2015 | 25652607 |