Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| tiamulin feed premix in the prevention and control of swine dysentery under farm conditions in the uk. | three field trials were carried out to evaluate the efficacy of tiamulin hydrogen fumarate at levels of 20 and 30 ppm in the feed for the prevention and control of swine dysentery. tiamulin at 30 ppm was shown to be highly effective in preventing the clinical signs of dysentery. treponema hyodysenteriae was not isolated from these pigs. tiamulin at 20 ppm was effective in two out of three trials. the trials were performed on pigs either on or from farms with swine dysentery problems which had pr ... | 1982 | 7043884 |
| isolation of treponema hyodysenteriae from wild rodents. | rodents from swine-producing farms were examined for the presence of treponema hyodysenteriae. wild mice (n = 257) and rats (n = 41) were trapped on eight farms. ceca were removed aseptically, and the contents and mucosal scrapings were cultured on selective medium (blood agar containing 400 micrograms of spectinomycin per ml). t. hyodysenteriae was detected in the cecal scrapings of four mice from three different farms where swine dysentery had occurred. gross lesions were detected in the ceca ... | 1982 | 7050159 |
| [mortality in sows caused by treponema hyodysenteriae]. | 1982 | 7054924 | |
| swine dysentery: the influence of dietary vitamin e and selenium on the clinical and pathological effects of treponema hyodysenteriae infection in pigs. | twenty-four conventionally reared pigs were divided into four equal groups and fed a basic ration deficient in vitamin e and selenium. one group was given a supplement of vitamin e and selenium. one group was given a supplement of vitamin e, another received selenium and a third received both nutrients. no supplement was given to the control group. after the pigs had been fed the different diets for 59 days they were inoculated with a pure culture of treponema hyodysenteriae they were subsequent ... | 1982 | 7089385 |
| tiamulin water medication in the treatment of swine dysentery under farm conditions. | the clinical efficacy of tiamulin given in the drinking water at levels of 0.0045 per cent for five days and 0.006 per cent for three days was evaluated for the treatment of swine dysentery in six clinical studies and compared with positive controls using tylosin at 0.02 per cent in the drinking water for five days and untreated controls. efficacy was evaluated by assessment of the pigs' general appearance; the extent of diarrhoea and dehydration; performance, as measured by average daily gains ... | 1982 | 7090152 |
| biological activity of a lipopolysaccharide extracted from treponema hyodysenteriae. | a lipopolysaccharide (lps) was obtained from pathogenic treponema hyodysenteriae by hot phenol-water extraction. various effects of the lps on host cells were examined in vitro. toxicity for mouse peritoneal macrophages was observed after 10 h of incubation at concentrations as low as 15 micrograms of the lps per ml. marked enhancement of both complement (c3) and immunoglobulin g-fc receptor-mediated internalization was noted in macrophages obtained from mice injected 6 days previously with 75 m ... | 1982 | 7107001 |
| serotype-specific opsonization of treponema hyodysenteriae. | treponema hyodysenteriae was shown to attach to mouse peritoneal cells in the absence of serum opsonins in vitro. if serotype-specific antiserum from pigs was added to the media and treponemes of that corresponding serotype were employed in the assay, the amount of attachment increased an average of 3.7 times that of the control without pig sera. however, the amount of attachment was increased an average of only 1.5 times that of the control if organisms of any noncorresponding serotype of t. hy ... | 1982 | 7152660 |
| toxigenicity of treponema hyodysenteriae, another emerging enteropathogen. | 1982 | 7174114 | |
| identification of treponema hyodysenteriae by a rapid slide agglutination test. | a rapid slide agglutination (sa) test was developed to identify the spirochaete treponema hyodysenteriae, the causative organism of swine dysentery. the specificity of the antiserum was increased by a single absorption with two intestinal spirochaetes. using this test, it was possible to identify 30 out of 31 spirochaetes which were beta-haemolytic and gave a positive reaction in growth inhibition (gi) tests with t hyodysenteriae antiserum. all except one of these spirochaetes were isolated from ... | 1981 | 7210456 |
| investigation of a hemolysin produced by enteropathogenic treponema hyodysenteriae. | a hemolysin produced by treponema hyodysenteriae, the etiological agent of swine dysentery, was investigated. a virulent isolate (b204) was inoculated into a standard culture medium consisting of trypticase soy broth without dextrose (bbl microbiology systems) supplemented with 10% fetal calf serum in an atmosphere of 70:30 deoxygenated h2-co2. sterile cell-free filtrates were prepared at 2-h intervals and assayed for hemolytic activity by using washed sheep erythrocytes. the maximum hemolytic t ... | 1981 | 7216445 |
| production of lesions in gnotobiotic mice by inoculation with treponema hyodysenteriae. | treponema hyodysenteriae was established in the ceca of gnotobiotic mice in the absence of other organisms. superficial mucosal lesions characteristic of swine dysentery were present in the ceca of mice inoculated with t. hyodysenteriae in combination with viable bacteroides vulgatus. deep crypt necrosis was detected in the ceca of mice inoculated with t. hyodysenteriae alone. | 1981 | 7216455 |
| microscopic agglutination test for antibody against treponema hyodysenteriae. | 1980 | 7254362 | |
| shedding of treponema hyodysenteriae, transmission of disease, and agglutinin response to pigs convalescent from swine dysentery. | fecal shedding of treponema hyodysenteriae, transmission of disease, and humoral antibody production against the large spirochete were evaluated in pigs convalescent from experimentally induced swine dysentery. untreated pigs (n = 21) and 5 pigs that had been treated with virginiamycin were included in the study. treponema organisms were culturally detected in the feces of 2 untreated pigs as long as 70 and 71 days, and in the feces of 1 treated pig as long as 83 days after the last clinical evi ... | 1981 | 7271009 |
| effects of aflatoxin consumption on the clinical course of swine dysentery. | specific-pathogen-free pigs were examined for susceptibility to swine dysentery after daily treatment with aflatoxin b1 (0.07 to 0.14 mg/kg). in the pigs (group ii) given both aflatoxin and treponema hyodysenteriae, the incubation period before the onset of swine dysentery was shorter than that in pigs (group iii) given t hyodysenteriae alone. also, the number of days in which infected pigs showed signs of diarrhea and dysentery was greater in the group given aflatoxin and t hyodysenteriae than ... | 1981 | 7271036 |
| investigation into the spread of swine dysentery in 25 herds of east anglia and assessment of its economic significance in five herds. | of 25 outbreaks of swine dysentery investigated, 22 were considered to have resulted from the purchase of pigs from farms known to be infected, two from the introduction of treponema hyodysenteriae on the boots of the stockman, and one from the accidental entry of infected pigs into a 'closed' herd. the hidden costs of swine dysentery in terms of an increased food conversion ratio may be more than four times the cost of medication. a method of halting the spread of disease is described. | 1981 | 7324356 |
| induction of swine dysentery in swine by the intravenous injection of filtered treponema hyodysenteriae. | swine dysentery was induced in 18 swine exposed by intravenous injection of a filtrate which contained treponema hyodysenteriae and was obtained from macerated colonic scrapings of swine dysentery. however, swine dysentery did not develop in swine injected intravenously with a pure culture of t. hyodysenteriae or when combined with a colonic filtrate from normal swine. diarrheal feces from the swine injected intravenously with the filtered t. hyodysenteriae contained more mucus, and fecal smears ... | 1981 | 7337868 |
| selective medium for the isolation of treponema hyodysenteriae. | 1981 | 7340069 | |
| studies on the properties of treponema hyodysenteriae with special regard to its systematic position. | 1981 | 7342737 | |
| sensitivity to different chemotherapeutics of treponema hyodysenteriae strains isolated in hungary. | 1981 | 7342743 | |
| lymphocyte stimulation in swine dysentery. | in lymphocyte stimulation studies of pigs affected with swine dysentery (sd) all of the pigs gave significant response (p less than 0.05) to soluble antigen from treponema hyodysenteriae. swine infected with virulent or attenuated t. hyodysenteriae gave significant lymphocytic response 3 or 6 weeks after infection; uninfected pigs did not give a similar lymphocytic response. the delayed hypersensitivity (dh) skin test, in which soluble t. hyodysenteriae antigen preparation was used, detected onl ... | 1981 | 7344261 |
| comparison of selective culture and serologic agglutination of treponema hyodysenteriae for diagnosis of swine dysentery. | samples of faeces and serum were collected from pigs of various ages on 21 farms. faecal samples were cultured on trypticase soy agar containing 5% citrated bovine blood and 400 microgram per ml spectinomycin, incubated at 42 degrees c in gaspak jars under an atmosphere of 80% hydrogen: 20% carbon dioxide. antibody titres to treponema hyodysenteriae were determined by a microtitration agglutination method using merthiolate-inactivated whole cell antigen prepared from a beta- haemolytic isolate. ... | 1980 | 7361391 |
| application of a leukocyte migration-inhibition agarose test to swine dysentery. | cell-mediated immunity (cmi) was demonstrated in swine naturally affected with swine dysentery (sd) by a leukocyte migration-inhibition agarose test (lmat). migration of leukocytes from sd-affected pigs was inhibited in the presence of soluble antigen of treponema hyodysenteriae, whereas migration of leukocytes from noninfected swine was not inhibited by exposure to the same antigen. cell-mediated immunity was detected initially after the appearance of clinical signs of the disease, and a peak m ... | 1980 | 7369605 |
| sterol requirement for the growth of treponema hyodysenteriae. | the addition of cholesterol to a liquid medium containing bovine serum albumin (bsa) fraction v or acetone-delipidized bsa fraction v instead of serum stimulated the growth of treponema hyodysenteriae, a serum-requiring spirochaete associated with swine dysentery. as little as 1.25 micrograms cholesterol ml-1 increased viable counts about 1000-fold. sitosterol and cholestanol, but not pregnenalone, cholestenone or stigmasteriol, produced a growth response comparable to that of cholesterol. the r ... | 1980 | 7373284 |
| production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from treponema hyodysenteriae. | a weakly beta-haemolytic spirochaete, isolate p43/6/78, was isolated from a pig with diarrhoea and found not to fluoresce with a specific fluorescent antiserum to treponema hyodysenteriae. pure cultures of this spirochaete were used to inoculate experimental pigs. diarrhoea, containing clear mucus, and, in one case, blood occurred in four of the eight animals inoculated. colitis was present in six of the eight inoculated pigs at necropsy. excess clear mucus and punctate haemorrhages were seen on ... | 1980 | 7376384 |
| alk-1-enyl ether phospholipids (plasmalogens) and glycolipids of treponema hyodysenteriae. analysis of acyl and alk-1-enyl moieties. | the lipids of treponema hyodysenteriae b78, the etiologic agent of swine dysentery, comprised 16.4% of the cell dry weight, and consisted of 37.4% glycolipids, 28.6% phospholipids, and 34.0% neutral lipids. monogalactosyldiacylglycerol, a major lipid in all treponema except treponema pallidum, comprised 80% of the glycolipids. an unidentified galactolipid less polar than monogalactosyldiacylglycerol was also detected. phosphatidylglycerol (19.5% of the total lipids) was the major phospholipid. p ... | 1980 | 7378438 |
| treponema innocens lipids and further description of an unusual galactolipid of treponema hyodysenteriae. | the lipids of treponema innocens, type strain b256, formerly considered a nonpathogenic isolate of t. hyodysenteriae, have been analyzed and compared with the lipids of t. hyodysenteriae. the lipids of t. innocens comprised 16% of the cell dry weight. polar lipids amounted to about two-thirds of the total lipids and consisted of 61.9% phospholipids and 38.1% glycolipid. neutral lipids consisted mainly of sterols. the phospholipids were principally phosphatidylglycerol, phosphatidylcholine, and c ... | 1980 | 7410314 |
| swine dysentery. comparison of experimental diseases produced by infection with colonic mucosa or with treponema hyodysenteriae, french strains, and of "natural" disease. | oral inoculation of colonic mucosa scrapings and intestinal contents of animals affected with swine dysentery, or of pathogenic strains of treponemia hyodysenteriae, as well as spontaneous contamination in infected pens caused in average swine dysentery to appear in 359 out of 409 spf piglets. the morbidity is high irrespective of the method of contamination: after pen contamination, 75 out of 83 piglets were dysenteric; after only one ingestion of contaminated matter, 265 out of 280 animals wer ... | 1980 | 7436331 |
| experimental transmission of treponema hyodysenteriae from mice to pigs. | transmission experiments with treponema hyodysenteriae were conducted with mice and pigs, using laboratory mice as the carrier host. mice were intragastrically inoculated with t hyodysenteriae and placed in a contact with nonexposed mice or healthy pigs. contact mice mingled with infected mice at 5 or 180 days after inoculation were shedding t hyodysenteriae in their feces 9 to 14 days after exposure. pigs exposed directly to infected mouse feces were shedding t hyodysenteriae in their feces aft ... | 1980 | 7447116 |
| a comparative study of spirochaetes from the porcine alimentary tract. | strains of treponema hyodysenteriae capable of inducing swine dysentery in specific pathogen-free pigs were compared with other spirochaetes from the porcine alimentary tract by biochemical and serological tests and by electrophoresis of their proteins. carbohydrate fermentation and esculin hydrolysis were similar in all the spirochaetes. indole was produced by t. hyodysenteriae and by some of the other spirochaetes. analysis of the fatty acids produced from glucose showed a difference between t ... | 1981 | 7462601 |
| differentiation of treponema hyodysenteriae from t innocens by enteropathogenicity testing in the cf1 mouse. | fourteen isolates of treponema hyodysenteriae and 11 isolates of t innocens from eight different countries were evaluated in the cf1 strain female mouse for virulence. mice were fasted for 24 hours and inoculated intragastrically with 1 ml of culture for two consecutive days. mice were killed and necropsied at 12 to 15 days after inoculation. caecitis was detected in mice from each of the groups receiving t hyodysenteriae (67 per cent) but not in mice receiving t innocens or sterile broth. lesio ... | 1980 | 7467101 |
| purification and characterization of treponema hyodysenteriae hemolysin. | a hemolysin produced by treponema hyodysenteriae atcc27164 was purified from broth filtrates by acetic and (nh4)2so4 precipitations followed by ion exchange chromatography on diethylaminoethyl-sephacel and gel filtration using ultrogel aca44. the purified hemolysin displayed only one band on polyacrylamide gel electrophoresis. by gel filtration the molecular weight was estimated as 74,000 daltons. the isolated hemolysin was oxygen resistant, heat labile and was not inactivated over a wide range ... | 1980 | 7470509 |
| characteristics of the interaction of a treponemal hemolysin with rabbit erythrocytes. | the mechanism of action on rabbit red cells of treponema hyodysenteriae hemolysin was studied using volume analysis and release of hemoglobin. while fixation of the hemolysin on the erythrocytes is temperature independent, it appears that hemolysis is temperature dependent. the kinetics of hemolysis proceed according to a sigmoid curve characterized by a prelytic lag. the duration of the prelytic lag varies inversely with the quantity of hemolysin but the rate and the maximum value of hemolysis ... | 1980 | 7470510 |
| survival of serpulina hyodysenteriae in an effluent lagoon. | to determine the survival of serpulina hyodysenteriae in an infected lagoon that received effluent from a confinement building housing swine dysentery-infected swine. | 1995 | 7493879 |
| production and characterization of monoclonal antibodies against serpulina hyodysenteriae and s. innocens and their use in serotyping. | murine monoclonal antibodies (mabs) directed against serotypes 1, 2, 8, and 9 of serpulina hyodysenteriae and strain b256 of serpulina innocens were produced and characterized. a serological classification of 96 field strains of s. hyodysenteriae and 28 field strains of s. innocens isolated from pigs showing clinical signs of swine dysentery was performed by rapid dot enzyme-linked immunosorbent assay (elisa) with the mabs. the results indicated that the majority of the field strains of s. hyody ... | 1995 | 7494066 |
| distribution of the smpa gene from serpulina hyodysenteriae among intestinal spirochaetes. | forty intestinal spirochaete strains were investigated for nucleotide sequences related to the smpa locus from serpulina hyodysenteriae by southern hybridization of chromosomal dna using the smpa locus from s. hyodysenteriae strain p18a as a probe and by pcr using primers internal to the smpa gene. the intensity of the hybridization signal at high stringency and positive pcr results suggested that 12 s. hyodysenteriae strains possessed a similar nucleotide sequence. pcr was negative for another ... | 1995 | 7496514 |
| the use of 16s rdna sequence analysis to investigate the phylogeny of leptospiraceae and related spirochaetes. | the 16s rdna sequences from 15 leptospiraceae were determined by automated pcr-directed cycle sequencing. nucleotide comparisons, including those from published sequences for leptospira canicola moulton and serpulina spp., were used to construct phylogenetic trees. serpulina hyodysenteriae and s. innocens were related to each other but were distinct from the leptospiraceae comprising leptospira parva incertae sedis (turneria parva h), leptonema illini and leptospira spp. the pathogenic and the s ... | 1993 | 7506293 |
| characterization of serpulina hyodysenteriae isolates of serotypes 8 and 9 from quebec by restriction endonuclease fingerprinting and ribotyping. | this study was undertaken to assess the discriminatory value of restriction endonuclease fingerprinting (ref) analysis and ribotyping of 21 serpulina hyodysenteriae isolates of serotypes 8 and 9. for ref analysis, dnas were digested with the bglii restriction enzyme and the resultant fragments were separated by polyacrylamide gel electrophoresis. for ribotyping, hybridization of bglii genomic fragments with a probe of rrnb operon using an escherichia coli rdna probe was performed on all isolates ... | 1994 | 7534207 |
| evaluation of different serological tests for detection of antibodies against serpulina hyodysenteriae in pig sera. | swine dysentery is a mucohemorrhagic diarrheal disease caused by s. hyodysenteriae. the detection of asymptomatic carriers in herds is possible by serological tests. however, cross-reactions between s. hyodysenteriae and s. innocens pose a major problem in serological diagnosis. several serological tests were evaluated for detection of antibodies to s. hyodysenteriae such as: indirect hemagglutination, passive hemolysis, conglutination and microagglutination tests. among the tests used, only the ... | 1995 | 7554822 |
| production and characterization of monoclonal antibodies specific for lipooligosaccharide of serpulina hyodysenteriae. | serpulina (treponema) hyodysenteriae is the causative agent of swine dysentery, a contagious mucohemorrhagic disease of the colon. diagnosis of swine dysentery is extremely difficult because of the presence of cross-reactive antibodies to the proteins of s. hyodysenteriae and serpulina innocens, a nonpathogenic inhabitant of the porcine large intestine. therefore, monoclonal antibodies (mabs) against the serotype-specific lipooligosaccharide (los) antigens of s. hyodysenteriae were produced to r ... | 1995 | 7559965 |
| certain canine weakly beta-hemolytic intestinal spirochetes are phenotypically and genotypically related to spirochetes associated with human and porcine intestinal spirochetosis. | four canine weakly beta-hemolytic intestinal spirochetes associated with intestinal spirochetosis (is-associated wbhis) were compared with is-associated human and porcine wbhis and the type species for serpulina hyodysenteriae and s. innocens by using phenotypic and genotypic parameters. the is-associated canine, human, and porcine wbhis belonged to a phyletic group distinct from but related to previously described serpulina type species. | 1995 | 7559984 |
| inactivation of serpulina hyodysenteriae flaa1 and flab1 periplasmic flagellar genes by electroporation-mediated allelic exchange. | serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class a and class b polypeptides. to examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the importance of motility in the etiology of this pathogen, we constructed specific periplasmic flagellar mutations in s. hyodysenteriae b204. the cloned flaa1 and flab1 genes were disrupt ... | 1995 | 7592350 |
| dna probe and polymerase chain reaction procedure for the specific detection of serpulina hyodysenteriae. | serpulina (treponema) hyodysenteriae, a gram-negative anaerobic spirochete, is the causative agent of swine dysentery, a mucohaemorrhagic diarrheal disease in which lesions are confined to the large intestine of pigs. a dna probe and polymerase chain reaction (pcr) amplification procedures which are specific, rapid , and sensitive for the detection of s.hyodysenteriae have been developed. clone pf12 from a plasmid library of s.hyodysenteriae b204 genomic dna was identified as a clone specific fo ... | 1995 | 7603470 |
| growth of serpulina (treponema) hyodysenteriae under iron-restricted conditions. | reference strains of serpulina hyodysenteriae expressed at least three iron-regulated proteins with apparent molecular masses of > 200, 134, and 109 kda when grown under iron-restricted conditions. cells of s. hyodysenteriae grown under these conditions also showed increased outer membrane bleb formation when examined by electron microscopy after negative staining. s. hyodysenteriae did not use the 2 most common types of siderophore, namely catechol and hydroxamate. western blotting with serum f ... | 1995 | 7648528 |
| the cytotoxic properties of serpulina hyodysenteriae. | examination of colonic enterocytes inoculate with pure culture of s. hyodysenteriae by phase-contrast microscopy revealed that only few spirochaetes adhere to epithelial cells. s. hyodysenteriae was observed to be highly motile, showed corkscrew-like movement which might suggest that bacteria were trying to penetrate and damaged the host cells. the pattern of motility provide evidence of a chemotaxis. supernatant of s.hyodysenteriae lysate were found to cause cte in cho, vero and pk-15 culture. ... | 1995 | 7660860 |
| expression of the smpa outer membrane lipoprotein of serpulina hyodysenteriae strain p18a in vivo. | an elisa has been developed using a monoclonal antibody (f325 ac4) to the smpa surface lipoprotein of serpulina hyodysenteriae strain p18a when grown in vitro. the lower level of detection of the elisa was approximately 5 x 10(6) spirochaetes/ml when spirochaetes were either resuspended in phosphate buffered saline or in pig faeces. when pigs were challenged with s. hyodysenteriae strain p18a the lipoprotein was detected in the faeces of pigs by elisa when the numbers of spirochaetes excreted wa ... | 1995 | 7667904 |
| serpulina hyodysenteriae challenge of fattening pigs vaccinated with anadjuvanted bivalent bacterin against swine dysentery. | an experimental fattening period was performed with 36 pigs in order to evaluate the safety and efficacy of a bivalent adjuvanted serpulina hyodysenteriae bacterin. the pigs originated from three different farms and were split into six groups. eighteen pigs were vaccinated twice, two weeks apart and the rest served as controls. one unvaccinated pig in each group was inoculated intragastrically with 4 x 10(8) active s. hyodysenteriae spirochaetes, b 78 strain. weight evolution, clinical symptoms, ... | 1995 | 7668035 |
| susceptibility of porcine ileal enterocytes to the cytotoxin of serpulina hyodysenteriae and the resolution of the epithelial lesions: an electron microscopic study. | the cytotoxin from serpulina hyodysenteriae was injected into ileal loops of eight germ-free pigs, and the effects on the villi were observed after 1, 3, and 18 hours of exposure. the mature vacuolated villus enterocytes of the proximal part of the absorptive villi were most susceptible to the lethal effects of the cytotoxin and were extensively exfoliated. the enterocytes at the base of the villi, the goblet cells, and the follicle-associated epithelium of the dome villi, particularly the m cel ... | 1995 | 7725595 |
| comparative study of the enzyme activities of borrelia burgdorferi and other non-intestinal and intestinal spirochaetes. | comparative analysis of the enzymatic profiles of 58 spirochaetal isolates clearly differentiated borrelias from leptospires, serpulinas and a treponeme. strains of both borrelia burgdorferi and borrelia hermsii characteristically produced significant amounts of leucine arylamidase. this enzyme activity was not unique to borrelias but was also detected amongst pathogenic and non-pathogenic leptospira serovars. this fact, however, did not hamper a correct differentiation of borrelias from these s ... | 1995 | 7760753 |
| prophylactic effect of dietary zinc in a laboratory mouse model of swine dysentery. | reduced prevalence of diarrhea and mortality has been reported after dietary supplementation with zinc compounds in swine with naturally acquired colibacillosis and those challenge-exposed with serpulina hyodysenteriae; however, the usefulness of this approach for control of enteric diseases of swine remains to be determined. to examine the effect of dietary zinc-containing compounds on the colonization and development of cecal lesions associated with s hyodysenteriae infection, a defined diet a ... | 1995 | 7771701 |
| use of commercial enzyme kits and fatty acid production for the identification of serpulina hyodysenteriae: a potential misdiagnosis. | the accuracy of identification of serpulina hyodysenteriae strains grown in a complex medium was 90% when 2 commercial test kits were used. unlike the other s. hyodysenteriae strains, s. hyodysenteriae strain p35/2 was unusual in being indole negative. the nonpathogenic intestinal spirochete pws/a, which is from a different species, was indole positive and alpha-galactosidase negative. identification of these spirochetes on the basis of these kits alone would have been incorrect. the analysis of ... | 1995 | 7779972 |
| effect of divalent cations on hemolysin synthesis by serpulina (treponema) hyodysenteriae: inhibition induced by zinc and copper. | serpulina hyodysenteriae produces an oxygen-stable heat-labile hemolysin that may be an important virulence factor in the pathogenesis of swine dysentery. we examined the effect of ca2+, co2+, cu2+, fe2+, mg2+, mn2+, ni2+, and zn2+ on the hemolytic activity of cell-free supernatant (cfs) from s. hyodysenteriae, isolate b204. cells harvested from late logarithmic phase cultures were incubated in phosphate-buffered saline containing glucose and rna-core (pbs-gr) with or without cations and the hem ... | 1994 | 7801526 |
| isolation of extracytoplasmic proteins from serpulina hyodysenteriae b204 and molecular cloning of the flab1 gene encoding a 38-kilodalton flagellar protein. | extracytoplasmic proteins were released from serpulina (treponema) hyodysenteriae (strain b204) by treatment of whole cells with a nonionic detergent (tween 20). centrifugation of the tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, 42, 39, 38, 34, 33.5, 33, 31, and 29 kda. treatment of the sedimented fraction with 6 m urea solubilized all of the proteins except the 39-kda protein. peptide sequences were obtain ... | 1995 | 7806350 |
| identification of a new intestinal spirochete with pathogenicity for chickens. | two intestinal spirochete isolates obtained from chickens with diarrhea were examined by electron microscopy, biochemical tests, rrna gene restriction pattern analysis, and multilocus enzyme electrophoresis. one isolate (strain 91-1207/c1) was pathogenicity tested in vivo in chickens. the chicken spirochetes were morphologically indistinguishable from serpulina innocens and serpulina hyodysenteriae and phenotypically similar to s. innocens. however, the chicken spirochetes could be distinguished ... | 1995 | 7822007 |
| serogroups of australian isolates of serpulina hyodysenteriae. | 1994 | 7848186 | |
| morphometric analysis of enteric lesions in c3h/hen mice inoculated with serpulina hyodysenteriae serotypes 2 and 4 with or without oral streptomycin pretreatment. | the segmental distribution and sequential progression and the role of the indigenous bacterial flora in the development of enteric lesions associated with serpulina hyodysenteriae infection in laboratory mice have not been defined. we examined the distribution and sequential morphometric changes in the large intestine of mice orally inoculated with s. hyodysenteriae serotypes 2 and 4. to determine the role of colonization resistance conferred by the indigenous bacterial flora, 40 female c3h/hen ... | 1994 | 7889460 |
| comparison of serpulina hyodysenteriae b78, the type strain of the species, with other s. hyodysenteriae strains using enteropathogenicity studies and restriction fragment length polymorphism analysis. | the enteropathogenicity of serpulina hyodysenteriae b78, the type strain of the species, was compared with s. hyodysenteriae b204, a known pathogenic strain, in 7 week-old pigs. clinical signs of swine dysentery were observed in 11/18 pigs (61.1%) inoculated with s. hyodysenteriae strain b204. however, in pigs inoculated with s. hyodysenteriae b78, only 1/21 (4.8%) of the pigs became infected. the 21 pigs inoculated with strain b78 included four pigs which received 5-fold higher numbers of s. hy ... | 1993 | 7903832 |
| identification and characterization of serpulina hyodysenteriae by restriction enzyme analysis and southern blot analysis. | chromosomal dna restriction enzyme analysis and southern blot hybridization were used to characterize serpulina hyodysenteriae strains. when chromosomal dnas from selected strains (reference serotypes) of s. hyodysenteriae were digested with the restriction endonuclease sau3a and hybridized with a 1.1-kb s. hyodysenteriae-specific dna probe, a common 3-kb band was always detected in s. hyodysenteriae strains but was absent from serpulina innocens strains. when the chromosomal dna was digested wi ... | 1994 | 7914209 |
| production of an inducible sucrase activity by serpulina hyodysenteriae. | strains of serpulina hyodysenteriae and serpulina innocens produced a cell-associated sucrase activity when grown in a medium containing sucrose. s. hyodysenteriae b204 sucrase activity cleaved sucrose and, to a lesser extent, raffinose and had a ph optimum of 5.7 to 6.2. this is the first report of an inducible enzyme produced by either s. hyodysenteriae or s. innocens. | 1994 | 7944375 |
| antimicrobial susceptibility testing of serpulina hyodysenteriae. | the macrobroth dilution technique was used to test the in-vitro effectiveness of 4 commonly used antimicrobial agents against 23 australian isolates and 7 overseas strains of serpulina hyodysenteriae. minimum inhibitory concentrations and minimum bactericidal concentrations were determined. the growth of 90% of isolates was inhibited by dimetridazole at a concentration of 4 micrograms/ml, and by tiamulin at 8 micrograms/ml. australian isolates resistant to both antimicrobial agents were identifi ... | 1994 | 7945100 |
| characterization of three putative serpulina hyodysenteriae hemolysins. | serpulina hyodysenteriae hemolysin is though to be an important virulence factor in swine dysentery. one gene, tlya, previously called tly, encoding a hemolysin in s. hyodysenteriae strain b204 has been characterized (muir et al. infect immun 1992; 60: 529-35). two other genes of s. hyodysenteria strain b204, designated tlyb and tlyc, encoding hemolytic proteins in escherichia coli strain dh5 alpha were cloned and sequenced. the tlyb and tlyc genes, when expressed in e. coli, encode heat-labile, ... | 1994 | 7968456 |
| evaluation of microagglutination test for differentiation between serpulina (treponema) hyodysenteriae and s. innocens and serotyping of s. hyodysenteriae. | swine dysentery is a mucohemorrhagic diarrheal disease caused by the anaerobic spirochete serpulina hyodysenteriae. at present, the serotyping is done by immunodiffusion testing with lipopolysaccharide (lps) extract as antigen and rabbit hyperimmune sera produced against different serotypes of s. hyodysenteriae. since the preparation of lps is time-consuming and requires a large quantity of bacteria, it is desirable to use a serotyping method which does not require the extraction of lps. in the ... | 1994 | 7989552 |
| chemotactic response to mucin by serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization. | chemotaxis of porcine spirochetes towards a variety of mucins was measured quantitatively by a capillary method. a chemotaxis buffer consisting of 0.01 m potassium phosphate buffer (ph 7.0) and 0.2 mm l-cysteine hydrochloride was necessary for chemotaxis of spirochetes. the optimum incubation time and incubation temperature were 1 h and 40 degrees c, respectively. the mucin concentration also affected the chemotaxis observed, and a concentration of 1% (wt/vol) was near the optimum. virulent serp ... | 1994 | 8063433 |
| rapid detection of serpulina hyodysenteriae in diagnostic specimens by pcr. | a pcr assay for the detection of serpulina hyodysenteriae in diagnostic specimens was developed on the basis of sequence analysis of a recombinant clone designated pred3c6. clone pred3c6, which contained a 2.3-kb dna fragment unique to s. hyodysenteriae, was identified by screening a plasmid library of s. hyodysenteriae isolate b204 genomic dna in escherichia coli by colony immunoblot with the mouse monoclonal antibody 10g6/g10, which was produced against cell-free supernatant antigens from the ... | 1994 | 8077394 |
| genetic similarity of intestinal spirochetes from humans and various animal species. | the chromosomal dna of spirochetes isolated from human, swine, dog, mouse, rat, and chicken intestine or feces was subjected to restriction enzyme analysis and hybridization with three different dna probes, derived from a flagellin gene, a hemolysin gene, and the 16s rdna sequence of the pathogenic swine intestinal spirochete serpulina hyodysenteriae. this genetic analysis showed that intestinal spirochetes represent a heterogeneous but related population of bacteria. in general, unique genotype ... | 1993 | 8096218 |
| physical and genetic map of the serpulina hyodysenteriae b78t chromosome. | a combined physical and genetic map of the serpulina hyodysenteriae b78t genome was constructed by using pulsed-field gel electrophoresis and dna blot hybridizations. the s. hyodysenteriae genome is a single circular chromosome about 3.2 mb in size. the physical map of the chromosome was constructed with the restriction enzymes bsshii, eclxi, noti, sali, and smai. the physical map was used to constructed a linkage map for genes encoding rrna, flagellum subunit proteins, dna gyrase, nadh oxidase, ... | 1994 | 8106320 |
| exacerbated onset of dysentery in swine vaccinated with inactivated adjuvanted serpulina hyodysenteriae. | after oral challenge exposure with serpulina hyodysenteriae-infected diced colon, fewer swine vaccinated with an experimental vaccine adjuvanted with mineral oil died (8 of 25 [32%]) than did nonvaccinated controls (6 of 15 [40%]), although the difference was not significant. however, onset and exacerbation of dysentery were accelerated in vaccinated swine because: 5 of the 8 dead vaccinated swine died before any of the nonvaccinates, which was significant (p < 0.01); vaccinated swine that died ... | 1994 | 8141499 |
| reduced virulence of serpulina hyodysenteriae hemolysin-negative mutants in pigs and their potential to protect pigs against challenge with a virulent strain. | the role of the serpulina hyodysenteriae hemolysin encoded by the tlya gene in the pathogenesis of swine dysentery (sd) was studied. tlya mutants of two s. hyodysenteriae strains (b204 and c5) were tested for virulence in pigs. none of the animals developed sd. however, after infection with wild-type strain b204 or c5, the incidence of sd was 100 or 60%, respectively. thus, the tlya-encoded hemolysin of s. hyodysenteriae is an important virulence factor in sd. the potential of these mutants to p ... | 1994 | 8188345 |
| pathogenicity of porcine intestinal spirochetes in gnotobiotic pigs. | twelve intestinal spirochete strains of porcine origin were characterized on the basis of their phenotypic properties, by multilocus enzyme electrophoresis, and by pathogenicity testing in gnotobiotic pigs. the spirochetes used included two strains of serpulina hyodysenteriae (b204 and p18a), two strains of serpulina innocens (b256 and 4/71), one strain from the proposed new genus and species "anguillina coli" (p43/6/78), and seven non-s. hyodysenteriae strains recently isolated from united king ... | 1994 | 8188364 |
| 16 kda envelope proteins in non-serpulina hyodysenteriae spirochaetes isolated from pigs. | spirochaetes isolated from field samples of diarrhoea, 'colitis' and mucoid diarrhoea from pigs were examined by a series of cultural, biochemical and serological tests. in addition sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to determine whether the organisms possessed a 16 kda protein thought to distinguish serpulina hyodysenteriae from s innocens. spirochaetal isolates which differed culturally and biochemically from s hyodysenteriae were found to possess a 16 kda prot ... | 1993 | 8236639 |
| cross-reactivity between b. burgdorferi and other spirochetes affects specificity of serotests for detection of antibodies to the lyme disease agent in dogs. | western immunoblots, the kinetics-based enzyme-linked immunosorbent assay (kela), and the microagglutination test were used to evaluate cross-reactivity among antibodies to serovars of leptospira interrogans (leptospiral serovars), and b. burgdorferi from naturally infected dogs, and to serpulina (treponema) hyodysenteriae from vaccinated rabbits. whole-cell lysates from borrelia spp., leptospiral serovars, and serpulina spp. were used for sds-page, western blots, and kela. crossreactivity occur ... | 1993 | 8236777 |
| a species-specific periplasmic flagellar protein of serpulina (treponema) hyodysenteriae. | we have previously reported that a 46-kda protein present in an outer membrane protein preparation seemed to be a species-specific antigen of serpulina hyodysenteriae (z. s. li, n. s. jensen, m. bélanger, m.-c. l'espérance, and m. jacques, j. clin. microbiol. 30:2941-2947, 1992). the objective of this study was to further characterize this antigen. a western blot (immunoblot) analysis and immunogold labeling with a monospecific antiserum against this protein confirmed that the protein was presen ... | 1993 | 8253687 |
| identification and partial characterization of a group of weakly beta-hemolytic intestinal spirochetes of swine distinct from serpulina innocens isolate b256. | comparative analyses of a group of 16 weakly beta-hemolytic spirochetes isolated from feces and mucosal scrapings of intestines of swine in the midwestern united states, and eastern canada revealed the existence of a phenotypically and genotypically related group of 7 isolates. although isolates in this group differed from all known reference isolates of intestinal spirochetes of swine, partial similarity was detected with s. joneseae isolate 16, a newly identified weakly beta-hemolytic intestin ... | 1993 | 8296452 |
| the role of hemolysin(s) in the pathogenesis of serpulina hyodysenteriae. | serpulina (treponema) hyodysenteriae, an anaerobic beta hemolytic spirochaete, is the etiologic agent of swine dysentery. not much is known at present about the virulence factors of s. hyodysenteriae. however, the hemolysin production of this bacterium is generally accepted to be a virulence factor. to study the exact role of hemolysin production in the pathogenesis of swine dysentery, the gene encoding a hemolysin, tly, was cloned and its nucleotide sequence determined. after inactivation of th ... | 1993 | 8347935 |
| characterization of two dna probes specific for serpulina hyodysenteriae. | two dna probes, one 1.1- and one 0.75-kb probe, specific for serpulina hyodysenteriae were isolated from a genomic library generated from virulent s. hyodysenteriae 5380. these probes are highly specific and react with all s. hyodysenteriae strains tested. under stringent conditions, the dna probes did not react with the nonpathogenic species serpulina innocens or with other species of enteric bacteria, including escherichia coli. both probes are able to detect s. hyodysenteriae in colony blot h ... | 1993 | 8349750 |
| comparison of outer-membrane fractions of serpulina (treponema) hyodysenteriae. | sarkosyl-insoluble fractions (outer-membrane proteins) and endoflagella (ef) fractions of serpulina hyodysenteriae serotypes 1-7 were examined for protein differences using sds-page. both the outer-membrane proteins (omp) and endoflagella were also examined for antigenicity using porcine sera from acutely infected and convalescent swine. seven major staining proteins were resolved in outer-membrane enriched fractions ranging in molecular weight between 42 and 32 kda. a comparison of purified ef ... | 1993 | 8362492 |
| genetic relationships between isolates of serpulina (treponema) hyodysenteriae, and comparison of methods for their subspecific differentiation. | multilocus enzyme electrophoresis (mee) was used to examine the extent of genetic diversity amongst 98 isolates of serpulina (treponema) hyodysenteriae. the species contained four major genetic divisions (a, b, c and d) and 29 electrophoretic types (ets). division d was relatively distinct, being separated from the other three divisions by fixed allelic differences at an average of 6.6 of 15 enzyme loci. electrophoretic differences were compared with results of dna restriction endonuclease analy ... | 1993 | 8383375 |
| molecular analysis of a flagellar core protein gene of serpulina (treponema) hyodysenteriae. | the flab2 gene encoding a protein located in the core of the periplasmic flagella of serpulina hyodysenteriae was cloned and sequenced. the flab2 protein consists of 285 amino acids and has a calculated molecular mass of 31.1 kda. southern blot analysis indicated that at least one, and possibly two genes related to flab2 are present in the genome of s. hyodysenteriae. comparison of the amino acid sequence of flab2 to sequences present in data banks showed significant similarity with the core fla ... | 1993 | 8409912 |
| human intestinal spirochetes are distinct from serpulina hyodysenteriae. | twenty-nine intestinal spirochetes isolated from australian aboriginal children and six strains from italian adults (hrm1, -2, -4, -5, -7, and -14) were genetically examined at 15 enzyme loci by using multilocus enzyme electrophoresis. results were compared with those previously obtained for 188 porcine intestinal spirochetes. dna from human strain hrm7 and porcine strain serpulina hyodysenteriae p18a were also radioactively labeled and hybridized with dna from 12 other human and porcine intesti ... | 1993 | 8417025 |
| molecular cloning, expression, and dna sequence analysis of the gene that encodes the 16-kilodalton outer membrane lipoprotein of serpulina hyodysenteriae. | the gene (smpa) that encodes the 16-kda outer membrane lipoprotein of serpulina hyodysenteriae was cloned in escherichia coli, and its primary structure was determined by nucleotide sequencing. the putative open reading frame encodes a prolipoprotein of 16.8 kda which in its fully acylated and cleaved form is 15.1 kda. analysis of the n-terminal amino acid sequence derived from the dna sequence revealed the presence of a signal sequence and a putative acylation and signal peptidase ii cleavage s ... | 1993 | 8432595 |
| experiences with a vaccine being developed for the control of swine dysentery. | a prototype vaccine that is being developed for the control of swine dysentery (sd) was tested in two groups of experimental pigs. vaccination induced high circulating antibody titres against the aetiological agent, serpulina (treponema) hyodysenteriae. pigs in the first trial were vaccinated twice before being challenged orally with the bacteria. five of 6 unvaccinated animals developed dysentery within a fortnight of challenge, but only 1 of 6 vaccinated pigs showed signs of disease at this ti ... | 1993 | 8460983 |
| purification and characterization of nadh oxidase from serpulina (treponema) hyodysenteriae. | nadh oxidase (ec 1.6.99.3) was purified from cell lysates of serpulina (treponema) hyodysenteriae b204 by differential ultracentrifugation, ammonium sulfate precipitation, and chromatography on anion-exchange, dye-ligand-affinity, and size-exclusion columns. purified nadh oxidase had a specific activity 119-fold higher than that of cell lysates and migrated as a single band during denaturing gel electrophoresis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis [sds-page]). the enzyme wa ... | 1993 | 8491717 |
| identification of a novel group of serpulina hyodysenteriae isolates by using a lipopolysaccharide-specific monoclonal antibody. | a monoclonal antibody to serpulina hyodysenteriae 8930 was produced and was used to probe pronase-treated cell lysates of s. hyodysenteriae isolates in immunblots. the results showed that the monoclonal antibody was specific for only five closely related s. hyodysenteriae isolates: 8930, 5380, 70a, rmit 88, and rmit 97. | 1993 | 8501237 |
| monitoring experimental swine dysentery: rectal swab blood test and serpulina (treponema) hyodysenteriae detection. | in two experiments, ten postweaning piglets were inoculated intragastrically with cultures of serpulina (treponema) hyodysenteriae strain b204 and were individually monitored for swine dysentery. eight control animals received sterile culture medium. between the day of inoculation and 35 days later, rectal swab samples were assayed for fecal blood by means of a commercial test kit and for s. hyodysenteriae cells by direct (phase contrast) microscopy and selective culture methods. optimal detecti ... | 1993 | 8506612 |
| comparison of the fatty acid profiles of borrelia, serpulina and leptospira species. | fatty acid methyl ester (fame) derivatives were examined as a means of characterizing borrelia burgdorferi isolates and distinguishing them from other spirochaetes. analysis was performed using a gas liquid chromatography column in conjunction with microbial identification system (mis) software. reproducible fame profiles were produced which distinguished borrelia species, serpulina hyodysenteriae and leptospira icterohaemorrhagiae. furthermore, the fame profiles of four recognized borrelia spec ... | 1993 | 8515244 |
| phenotypical characterisation of intestinal spirochaetes isolated from pigs. | a combined evaluation of the phenotypical properties of five serpulina type or reference strains and 163 swedish isolates of spirochaetes from pigs and two from birds was made. the porcine isolates were collected from herds with a history of dysentery or severe diarrhoea and from herds chosen at random. on the basis of beta-haemolysis, indole production, hippurate hydrolysis, and alpha-galactosidase, alpha-glucosidase and beta-glucosidase activity, the isolates could be divided into four main gr ... | 1995 | 8525077 |
| swine dysentery: more unknown than known. | swine dysentery (sd) is an economically important disease. it is caused by the spirochete serpulina hyodysenteriae. in order to minimize the economic damage of sd, several methods to control this disease are recommended. whereas hygienic measures and use of antimicrobials are actually practised for prevention, detection and exclusion of carriers of s. hyodysenteriae and vaccination against the disease still needs further attention. the last two methods require reliable and sensitive diagnostic t ... | 1995 | 8560732 |
| serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. | phenotypic and genetic traits of porcine intestinal spirochete strain p43/6/78t (= atcc 51139t) (t = type strain), which is pathogenic and weakly beta-hemolytic, were determined in order to confirm the taxonomic position of this organism and its relationships to previously described species of intestinal spirochetes. in bhis broth, p43/6/78t cells had a doubling time of 1 to 2 h and grew to a maximum cell density of 2 x 10(9) cells per ml at 37 to 42 degrees c. they hydrolyzed hippurate, utilize ... | 1996 | 8573497 |
| mitomycin c induction of bacteriophages from serpulina hyodysenteriae and serpulina innocens. | a prophage was induced from cells of the pathogenic spirochaete serpulina hyodysenteriae using mitomycin c. five to seven hours after mitomycin c was added (8 micrograms/ml, final concentration) to s. hyodysenteriae b204 cultures in bhis broth (od620 = 0.9) cell lysis was detected as a decrease in culture optical density. bacteriophage particles attached to whole cells and to cell debris were detected by electron microscopic analysis of negatively stained (2% pta, ph 7.0) bacteria harvested by c ... | 1995 | 8593962 |
| pigs experimentally infected with serpulina hyodysenteriae can be protected from developing swine dysentery by feeding them a highly digestible diet. | weaner pigs (n = 72) were fed 1 of 4 diets. these were based on either cooked rice and animal protein, cooked rice and lupin, wheat and lupin, or wheat and animal protein. twenty-six of the pigs were slaughtered after 1 month. those fed the highly digestible cooked rice and animal protein diet had drier colonic contents and faeces, lighter large intestines, and the contents of their large intestines had increased ph values and decreased total vfa concentrations. the other 46 were orally challeng ... | 1996 | 8620913 |
| sensitivity of strains of serpulina hyodysenteriae isolated in hungary to chemotherapeutic drugs. | the sensitivity of 332 strains of serpulina hyodysenteriae isolated in hungary between 1978 and 1992 was tested against seven chemotherapeutic drugs frequently used for the treatment of swine dysentery, and the changes in the patterns of resistance were also monitored. all the strains remained sensitive to carbadox, with minimum inhibitory concentrations (mic) of only 0.05 to 0.40 microgram/ml at present. the susceptibility of the strains to dimetridazole has gradually decreased, but about half ... | 1996 | 8677605 |
| identification and characterization of genus-specific epitopes of serpulina species using monoclonal antibodies. | four murine monoclonal antibodies (mabs) designated as c9e8, a10, g12, and g8 which recognized both serpulina hyodysenteriae and s. innocens were produced and characterized. the mabs reacted with whole cell antigens in elisa, indirect immunofluorescence and immunoblot assays. the mabs did not show any cross reactivity in rapid dot elisa or immunoblot assay with leptospira icterohemorrhagiae, campylobacter jejuni and escherichia coli. treatment of whole cell suspension with proteinase k and sodiu ... | 1996 | 8701579 |
| sensitivity of serpulina hyodysenteriae to tiamulin in the uk. | 1996 | 8711890 | |
| cell membrane permeability and mitochondrial dysfunction-inducing activities in cell-free supernatants from serpulina hyodysenteriae serotypes 1 and 2. | membrane permeability (mp) and mitochondrial dysfunction-inducing (mdi) activities were detected in cell-free supernatants (cfs) of serpulina hyodysenteriae, using either hemoglobin release from porcine red blood cells (rbc) or cytoplasmic lactate dehydrogenase release from porcine peripheral blood lymphocytes (pbl), and reduction of the 3-(4,5-dimethylthiazoyl-2-y1)-2,5-diphenyltetrazolium bromide dye by porcine pbl. the mp and mdi activities of cfs correlated with each other for serotype 1 and ... | 1996 | 8800549 |
| development of an experimental model allowing discrimination between virulent and avirulent isolates of serpulina (treponema) hyodysenteriae. | variation in virulence among different strains of serpulina hyodysenteriae was studied by oral inoculation of specific pathogen free piglets and cd-1 mice. piglets infected with serotype 2 reference strain b204 and an untypable field strain lhv-90-9-i had severe diarrhea tainted intermittently with mucus and fresh blood. the piglets inoculated with b169, b8044, b6933, and ack300-8 reference strains representing serotypes 3, 5, 6, and 7 respectively developed moderate diarrhea. however, reference ... | 1996 | 8825993 |
| motility and chemotaxis in serpulina hyodysenteriae. | chemotactic- or motility-regulated mucus association appears to be the predominant mechanism of mucosal association by the causative agent of swine dysentery, serpulina hyodysenteriae. in the present study, a modification of the adler capillary assay was used to evaluate the chemotactic responses of s. hyodysenteriae to a variety of potential stimuli. first, however, it became necessary to study factors that influenced motility of the spirochete in vitro, since standard cultivation methods produ ... | 1996 | 8861640 |
| differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16s rrna sequence comparisons. | multilocus enzyme electrophoresis (mee) analysis and comparisons of nearly complete 16s rrna gene sequences (1416 nucleotide positions) were used to evaluate phylogenetic relationships among serpulina hyodysenteriae strain b78t, s. innocens strain b256t, brachyspira aalborgi strain 513at, and eight uncharacterised strains of swine, avian, and human intestinal spirochaetes. from mee analysis, nine strains could be assigned to five groups containing other intestinal spirochaetes (genetic distances ... | 1996 | 8869502 |
| production and characterisation of a monoclonal antibody to serpulina hyodysenteriae. | a monoclonal antibody (mab) directed against serpulina hyodysenteriae, the causative agent of swine dysentery, was produced and characterised. the mab (bjl/sh1) reacted in western blots with a protein with a molecular mass of about 30 kda in outer membrane preparations from a range of s. hyodysenteriae isolates of different serotypes. it did not react with preparations made from a variety of non-s. hyodysenteriae intestinal spirochaetes. immunogold labelling was used to confirm the location of t ... | 1996 | 8869504 |
| the incidence of swine dysentery in pigs can be reduced by feeding diets that limit the amount of fermentable substrate entering the large intestine. | two experiments were conducted to test the hypothesis that feeding diets which limit the amount of fermentable substrate entering the large intestine would protect pigs against experimental infection with serpulina hyodysenteriae, the causative agent of swine dysentery. experiment 1 examined the effect of grain processing (hammer milling vs. steam flaking) and grain type (barley, groats, corn, sorghum and wheat) on indices of fermentation in the large intestine and the incidence of swine dysente ... | 1996 | 8914966 |
| dual flaa1 flab1 mutant of serpulina hyodysenteriae expressing periplasmic flagella is severely attenuated in a murine model of swine dysentery. | the motility imparted by the periplasmic flagella (pf) of serpulina hyodysenteriae is thought to play a pivotal role in the enteropathogenicity of this spirochete. the complex pf are composed of multiple class a and class b polypeptides. isogenic strains containing specifically disrupted flaal or flab1 alleles remain capable of expressing pf, although such mutants display aberrant motility in vitro. to further examine the role that these proteins play in the maintenance of periplasmic flagellar ... | 1996 | 8926083 |
| induction of interleukin (il)-1beta and il-8 mrna expression in porcine macrophages by lipopolysaccharide from serpulina hyodysenteriae. | lipopolysaccharide (lps) is a classic inducer of inflammatory cytokines and is a key virulence factor for most gram-negative pathogens. the effect of phenol-water (lps) and butanol-water (endotoxin) extracts from serpulina hyodysenteriae on inflammatory cytokine mrna expression from porcine alveolar macrophages was investigated. the lps and endotoxin extracts from s. hyodysenteriae induced a dose-dependent expression of interleukin 1beta (il-1beta) and il-8 which was weak compared with the respo ... | 1996 | 8926114 |