Publications

hypothalamic type ii iodothyronine deiodinase: a light and electron microscopic study.	in the central nervous system, the active form of thyroid hormone, t3, derives from the cellular uptake and intracellular 5'-monodeiodination of t4 by type ii 5'-monodeiodinase (dii). here, we report that using an antiserum raised against the c-terminus of the full-length sedii, immunolabeled cells were found in the rat hypothalamus in agreement with the dii mrna distribution. light and electron microscopy shows that dii is localized in astrocytes and tanycytes, supporting the hypothesis that th ...	2003	12763631
[surgical management of subglottic carcinoma].	to explore the clinical features and the methods of surgery and functional restoration of subglottic carcinoma.	1999	12764836
[supracricoid partial laryngectomy for laryngeal cancer].	to analyze the key points, especially the indications and contraindications of supracricoid partial laryngectomy (scpl).	1999	12764838
[cervical lymph node metastasis and recurrence in supraglottic carcinoma after operation].	to evaluate the regional control efficacy of elective neck dissection (end) in patients with supraglottic carcinoma.	1999	12764862
mutational analysis of bacteriophage t4 rna ligase 1. different functional groups are required for the nucleotidyl transfer and phosphodiester bond formation steps of the ligation reaction.	t4 rna ligase 1 (rnl1) exemplifies an atp-dependent rna ligase family that includes fungal trna ligase (trl1) and a putative baculovirus rna ligase. rnl1 acts via a covalent enzyme-amp intermediate generated by attack of lys-99 n zeta on the alpha phosphorus of atp. mutation of lys-99 abolishes ligase activity. here we tested the effects of alanine mutations at 19 conserved positions in rnl1 and thereby identified 9 new residues essential for ligase activity: arg-54, lys-75, phe-77, gly-102, lys ...	2003	12766156
off-resonance r1rho relaxation outside of the fast exchange limit: an experimental study of a cavity mutant of t4 lysozyme.	an (15)n off-resonance r(1rho) spin relaxation study of an l99a point mutant of t4 lysozyme is presented. previous cpmg-based relaxation dispersion studies of exchange in this protein have established that the molecule interconverts between a populated ground state and an excited state (3.4%) with an exchange rate constant of 1450 s(-1) at 25 degrees c. it is shown that for the majority of residues in this protein the offset dependence of the r(1rho) relaxation rates cannot be well fit using mod ...	2003	12766401
lymphoscintigraphic details of sentinel lymph node detection in 82 patients with squamous cell carcinoma of the oral cavity and oropharynx.	lymphoscintigraphy for sentinel node (sn) detection has been studied extensively in melanoma and breast cancer. in head and neck squamous cell carcinoma (hnscc), however, experience in this field is relatively meagre. the purpose of this study was to document and evaluate lymphoscintigraphic findings in hnscc patients. eighty-two patients with clinical t1-t4 n0 scc of the oral cavity or oropharynx received peritumoral injections of 25-75 mbq 99mtc-colloidal albumin (ca). dynamic lymphoscintigrap ...	2003	12766600
[screening for congenital hypothyroidism, phenylketonuria, galactosemia and biotinidase deficiency in a sample of mentally retarded patients in the city of havana].	congenital hypothyroidism (ch), phenylketonuria (pku), galactosemia (gal) and biotinidase deficiency (bd) are innate errors in metabolism that share varying degrees of mental retardation (mr) as a common characteristic. aims. the aim of our study was to screen individuals with mr of unknown origin for ch, pku, gal and bd.	2003	12766862
dose-threshold for thyroid tumor-promoting effects of xylazine in rats.	to clarify the threshold dose of thyroid tumor-promoting effects of xylazine hydrochloride (xz), male f344 rats received pulverized basal diet containing 0, 250, 500, or 1000 ppm xz for 26 weeks with or without initiation of 2400 mg/kg n-bis(2-hydroxypropyl)nitrosamine (dhpn). thyroid weights significantly increased in the groups with or without dhpn initiation that were given 500 ppm xz or more. the serum thyroxine (t4) and triiodothyronine (t3) levels decreased significantly in the xz 250 and ...	2003	12767697
an analysis of cervical lymph nodes metastasis in oral squamous cell carcinoma. relationship between grade of histopathological malignancy and lymph nodes metastasis.	cervical lymph nodes metastasis is well known to be an indicator of poor prognosis in patients with oral cancer. this study was considered 38 patients of oral squamous cell carcinoma who were treated at our department from 1993 to 1997. histological malignancy of initial biopsy in the pretreatment period was evaluated by anneroth's classification. the relationship of degree of histological malignancy with cervical lymph nodes metastasis were analysed in this study. metastasis in the cervical lym ...	2003	12767876
full-thickness thoracic laminar erosion after posterior spinal fusion associated with late-presenting infection.	a case report describes unilateral complete laminar erosion of the caudal thoracic spine and late-presenting infection in a patient 10 years after anteroposterior reconstruction for scoliosis.	2003	12768158
combined fractures of the odontoid process and upper thoracic spine: two case reports.	we describe two cases of combined fractures of the odontoid process and upper thoracic spine that have not been previously reported. the first patient, a 21-year-old man, sustained an odontoid process fracture with posterior displacement and a fracture-dislocation injury at t4/5 in a motorcycle accident. the second patient, a 66-year-old woman, fell from a cliff and sustained an odontoid process fracture with posterior displacement and a bursting fracture at t3 and t4 with rotation. the first pa ...	2003	12768489
[horizontal-vertical hemilaryngectomy for advanced laryngeal cancer].	to evaluate the adequacy of horizontal-vertical partial laryngectomy in the treatment of advanced laryngeal cancer.	2000	12768673
[laryngeal function-sparing surgery for pyriform sinus cancer].	to evaluate the oncologic effect of laryngeal function-sparing surgery for the patients with pyriform sinus carcinoma.	2000	12768706
[effect of microwave coagulation and surgery to treat the cancer of the floor of mouth].	the aim of this article was to clarify the value of microwave coagulation to treat the cancer of the floor of mouth by comparing the effect of microwave and surgery.	2002	12768714
[long-term follow-up result of partial laryngectomy].	to study the long-term follow-up result of partial laryngectomy and reservation of laryngeal function.	2000	12768742
molecular dynamics simulations of peptides and proteins with amplified collective motions.	we present a novel method that uses the collective modes obtained with a coarse-grained model/anisotropic network model to guide the atomic-level simulations. based on this model, local collective modes can be calculated according to a single configuration in the conformational space of the protein. in the molecular dynamics simulations, the motions along the slowest few modes are coupled to a higher temperature by the weak coupling method to amplify the collective motions. this amplified-collec ...	2003	12770868
the phage t4 restriction endoribonuclease regb: a cyclizing enzyme that requires two histidines to be fully active.	the regb gene, from the bacteriophage t4, codes for an endoribonuclease that controls the expression of a number of phage early genes. the regb protein cleaves its mrna substrates with an almost absolute specificity in the middle of the tertranucleotide ggag, making it a unique well-defined restriction endoribonuclease. this striking protein has no homology to any known rnase and its catalytic mechanism has never been investigated. here, we show, using 31p nuclear magnetic resonance (nmr), that ...	2003	12771201
hazard rates of disease progression after external beam radiotherapy for clinically localized carcinoma of the prostate.	we established hazard rates for disease progression at different intervals after external beam radiotherapy alone in patients with clinically localized prostate cancer. we determined the likelihood of biochemical failure in those free of disease 5 years after radiotherapy and identified those at risk for early versus late biochemical failure.	2003	12771739
role of clinically determined depth of tumor invasion in the treatment of esophageal carcinoma.	we sought to evaluate the effectiveness of clinical staging of depth of tumor invasion (ct), the relationship of ct to survival, the benefits of downstaging ct, and the role of ct in treatment decisions.	2003	12771883
[preservative surgery of t4 glottic cancer].	to explore new methods of preservative surgery for t4 glottic cancer and to evaluate their long term results.	2002	12772420
characterization of rat iodothyronine sulfotransferases.	sulfation appears to be an important pathway for the reversible inactivation of thyroid hormone during fetal development. the rat is an often used animal model to study the regulation of fetal thyroid hormone status. the present study was done to determine which sulfotransferases (sults) are important for iodothyronine sulfation in the rat, using radioactive t4, t3, rt3, and 3,3'-t2 as substrates, 3'-phosphoadenosine-5'-phosphosulfate (paps) as cofactor, and rat liver, kidney and brain cytosol, ...	2003	12773305
exposure to perfluorooctane sulfonate during pregnancy in rat and mouse. i: maternal and prenatal evaluations.	the maternal and developmental toxicities of perfluorooctane sulfonate (pfos, c8f17so3-) were evaluated in the rat and mouse. pfos is an environmentally persistent compound used as a surfactant and occurs as a degradation product of both perfluorooctane sulfonyl fluoride and substituted perfluorooctane sulfonamido components found in many commercial and consumer applications. pregnant sprague-dawley rats were given 1, 2, 3, 5, or 10 mg/kg pfos daily by gavage from gestational day (gd) 2 to gd 20 ...	2003	12773773
the different cardiac expression of the type 2 iodothyronine deiodinase gene between human and rat is related to the differential response of the dio2 genes to nkx-2.5 and gata-4 transcription factors.	by producing t3 from t4, type 2 iodothyronine deiodinase (d2) catalyzes the first step in the cascade underlying the effect exerted by thyroid hormone. type 2 iodothyronine deiodinase mrna is expressed at high levels in human heart but is barely detectable in the corresponding rodent tissue. although the heart is a major target of thyroid hormone, the role of cardiac d2 and the factors that regulate its expression are unknown. here we report that the human dio2 promoter is very sensitive to the ...	2003	12775767
morbidity and mortality after self-expandable metallic stent placement in patients with progressive or recurrent esophageal cancer after chemoradiotherapy.	placemet of self-expandable metallic stents in patients with advance esophageal cancer improves dysphagia and occludes tracheoesophageal fistulas. however, the safety of self-expandable metallic stents for patients who have undergone chemoradiotherapy is controversial. this study evaluated the morbidity and modality after self-expandable metallic stent placement in patients with progressive or recurrent esophageal cancer after chemoradiotherapy.	2003	12776036
unusual sites of colorectal cancer metastasis.	we present 2 separate cases of adenocarcinoma of the colon with metastasis to the chin and the bladder, both of which are unusual sites of colorectal cancer metastasis. patient 1 is a 77-year-old man who was diagnosed with adenocarcinoma of the colon, american joint committee on cancer (ajcc) t4 n0 m0 (stage ii), and underwent a right hemicolectomy. fourteen months later he developed a firm 2.5-cm mass involving the chin. excisional biopsy revealed moderately differentiated adenocarcinoma, consi ...	2003	12777193
fgf8 isoform b expression in human prostate cancer.	overexpression of fibroblast growth factor 8 (fgf8) mrna has been previously described in prostate cancer. of its four isoforms, fgf8b is thought to be the most important in carcinogenesis. we hypothesised that immunodetection of fgf8b in archival prostate cancer specimens is of potential prognostic value. using a selected cohort of prostate tumours from transurethral (n=30) and radical prostatectomies (n=59), an optimised protocol for fgf8b immunoreactivity was used to corroborate expression wi ...	2003	12778074
posttraumatic stress disorder: do electrical startle responses and thyroid function usefully supplement self-report? a study of vietnam war veterans.	to investigate the usefulness of electrical startle responses and thyroid function as supplements to self-report measures of posttraumatic stress disorder (ptsd).	2003	12780473
severe dysplastic lesions in the colon - how aggressive should we be?	introduction: biopsies of colonic lesions are often reported as showing dysplasia, though in reality some lesions may harbour invasive malignancy. aim: to assess the risk of underlying invasive malignancy in sessile polyps where biopsies had shown severe dysplasia and also to attempt to define a management strategy in such patients. methods: between 1997 and 2001, 30 patients were diagnosed as having severe dysplasia using morson's criteria in colonic lesions not amenable for endoscopic polypect ...	2002	12780579
factors affecting survival after palliative resection of colorectal carcinoma.	objective: to determine the factors affecting survival following palliative large bowel resection for colorectal adenocarcinoma. patients and method: from the colorectal cancer database of a single institution patients who had a palliative resection of a colorectal cancer from 1980 to 1993 inclusive were identified. survival curves were constructed using the kaplan-meier method. criteria studied were sex, age at operation, site of tumour, t, n and m status, tumour differentiation, involvement of ...	2002	12780652
pathological staging of epidermoid anal carcinoma for the new era.	chemoradiotherapy is the standard treatment for most patients with epidermoid anal cancer. pre-treatment staging is based on size for t1-t3 lesions and clinical and radiological assessment of adjacent organ invasion for t4 lesions. for patients with residual or recurrent carcinoma, anorectal excision offers the best chance of oncological salvage. pathological staging systems for anorectal excision specimens were validated at the time when surgical treatment was first line therapy. a validated st ...	2003	12780879
micrometastasis in regional lymph nodes of extirpated colorectal carcinoma: immunohistochemical study using anti-cytokeratin antibodies ae1/ae3.	the main objectives of this study were to identify, by immunohistochemistry, possible micrometastasis in the regional lymph nodes previously considered free by conventional histopathological examination, and to assess their influence on the survival of patients with colorectal cancer that had been extirpated in a radical manner.	2003	12780907
local recurrence in patients with large and locally advanced breast cancer treated with primary chemotherapy.	primary chemotherapy is being given in the treatment of large and locally advanced breast cancers, but a major concern is local relapse after therapy. this paper has examined patients treated with primary chemotherapy and surgery (either breast-conserving surgery or mastectomy) and has examined the role of factors which may indicate those patients who are subsequently more likely to experience local recurrence of disease.	2003	12781879
[squamous cell carcinoma of the lip. retrospective analysis of 42 cases].	to examine to what extent physicians follow a regional guidelines for the diagnosis, staging, treatment and follow-up of patients with squamous cell carcinoma of the lower and upper lip.	2003	12783065
staging of colonic neoplasms by colonoscopic miniprobe ultrasonography.	in contrast to the situation in the upper gastrointestinal tract staging of colonic neoplasm by endoscopic ultrasonography (eus) has not gained importance because until yet preoperative staging is without any clinical consequences. this may change with the introduction of minimally invasive surgical procedures and endoscopic resection techniques as an alternative to conventional (open) surgery.	2003	12783253
interaction of t4 endonuclease v with dna: importance of the flexible loop regions in protein-dna interaction.	t4 endonuclease v (t4 endo v), a thymine dimer-specific dna repair enzyme, and its interaction with dna were investigated by nuclear magnetic resonance (nmr) spectroscopy. backbone resonance assignment, chemical shift mapping, and 15n relaxation measurements were employed to the free and dna-bound enzymes. the secondary structure and the tertiary fold of t4 endo v in solution were consistent with those from the crystallographic study. the backbone 1h and 15n chemical shift perturbation upon the ...	2003	12783877
epidemiological typing of acanthamoeba strains isolated from keratitis cases in belgium.	from the corneas of nine keratitis patients and from their contact lenses, contact lens boxes and saline solutions, 15 strains of acanthamoeba have been isolated. an acanthamoeba strain was isolated from the swimming pool where one of the patients swam, while in the tapwater of the houses of three patients investigated, no acanthamoeba could be detected. all the acanthamoeba isolates from the cornea belong to genotype t4, but are different subtypes of t4. the acanthamoeba detected on the contact ...	2003	12784575
diagnosis and surgical treatment of thyroid adenoma-induced hyperthyroidism in a green iguana (iguana iguana).	an adult female green iguana (iguana iguana) presented with polyphagia, loss of the dorsal spines, hyperactivity, increased aggression, tachycardia, and a bilobate mass palpable anterior to the thoracic inlet. diagnosis of hyperthyroidism was based on a total t4 (30.0 nmol/l) elevated above that of clinically healthy iguanas (3.81 +/- 0.84 nmol/l), and histopathology confirmed a functional thyroid adenoma. surgical thyroidectomy safely returned the lizard to a euthyroid state.	2001	12785702
effects of selenium compounds on induction of dna damage by broadband ultraviolet radiation in human keratinocytes.	background ultraviolet radiation (uvr), a ubiquitous environmental genotoxin for the skin, produces dna damage. the trace element selenium induces synthesis of the glutathione peroxidase and thioredoxin reductase enzyme families. these selenoenzymes detoxify a range of toxic compounds generated by free radicals. objectives to assess the effects of pretreatment of primary human keratinocytes with selenium on uvr-induced dna damage. methods cells were irradiated with uvr from fs-20 lamps and were ...	2003	12786833
thyroxine downregulates sox9 and promotes chondrocyte hypertrophy.	thyroid hormones exert a profound effect on development, growth, and metabolism of skeleton. in the present study, we evaluated the effects of thyroxine (t4) and growth hormone (gh) on the terminal differentiation of rib growth plate chondrocytes in three-dimensional pellet culture. t4 (30ng/ml) stimulated the expressions of type ii and x collagens, alkaline phosphatase (alp) activity. on the other hand, the expression of chondrogenic transcription factor sox9 in the t4 treatment group decreased ...	2003	12788086
long-term changes in pulmonary function tests after definitive radiotherapy for lung cancer.	to evaluate the long-term changes in pulmonary function tests (pfts) in patients surviving at least 2 years after definitive radiotherapy (rt) for unresectable lung cancer.	2003	12788165
concurrent cisplatin, 5-fluorouracil, leucovorin, and radiotherapy for invasive bladder cancer.	to investigate the tolerance and efficacy of a modified concurrent chemoradiation (ccrt) protocol for patients with invasive bladder cancer "unfit" for radical cystectomy.	2003	12788178
accelerated superfractionated radiotherapy with concomitant boost for invasive bladder cancer.	to determine the toxicity and clinical effectiveness of accelerated superfractionated radiotherapy with delayed concomitant boost (ascbrt) in locally invasive carcinoma of the bladder.	2003	12788179
contribution of conformal therapy in the treatment of anal canal carcinoma with combined chemotherapy and radiotherapy: results of a phase ii study.	to evaluate the feasibility of delivering conformal radiotherapy (rt) and concurrent chemotherapy without a mandatory break in patients with anal canal carcinoma.	2003	12788191
hypothalamic-pituitary-thyroid system activity during lithium augmentation therapy in patients with unipolar major depression.	lithium augmentation is an established strategy in the treatment of refractory depression, but little is known about predictors of response and its mode of action. there is increasing evidence that low thyroid function indices within the normal range are associated with a poorer treatment response to antidepressants, but previous studies on the hypothalamic-pituitary-thyroid (hpt) system during lithium augmentation provide inconclusive results and have methodological limitations. this study aime ...	2003	12790161
new system for positive selection of recombinant plasmids and dual expression in yeast and bacteria based on the restriction ribonuclease regb.	by coupling the toxic restriction endoribonuclease regb, from the bacteriophage t4, to the prokaryotic t7 and the eukaryotic gal1 promoters, we constructed a two-function plasmid called ptoxr-1. this plasmid is a zero-background cloning vector. it allows an efficient positive selection of recombinant plasmids without the need to completely digest, dephosphorylate, or purify the vector prior to the ligation step. the ptoxr-1 positive selection system requires no special escherichia coli strains, ...	2003	12790631
several salmonella enterica subsp. enterica serotype 4,5,12:i:- phage types isolated from swine samples originate from serotype typhimurium dt u302.	pulsed-field gel electrophoresis, plasmid profiling, and phage typing were used to characterize and determine possible genetic relationships between 48 salmonella enterica subsp. enterica isolates of pig origin collected in catalonia, spain, from 1998 to 2000. the strains were grouped into 23 multidrug-resistant fljb-lacking s. enterica serovar 4,5,12:i:- isolates, 24 s. enterica serovar typhimurium isolates, and 1 s. enterica serovar 4,5,12:-:- isolate. after combining the xbai and blni macrore ...	2003	12791855
influence of identification and preservation of pelvic autonomic nerves in rectal cancer surgery on bladder dysfunction after total mesorectal excision.	given the improvement in oncologic outcome after the introduction of total mesorectal excision for the treatment of rectal cancer, the objective of the present study was to determine the frequency of identification and preservation of the pelvic autonomic nerves and to identify a possible link between postoperative micturition disturbances and the extent of the radical resection.	2003	12792438
neonatal hyperbilirubinemia and its association with thyroid hormone levels and urinary iodine excretion.	to investigate, if, urinary iodine contents as a marker of iodine deficiency and hypothyroidism are associated with the incidence of neonatal hyperbilirubinemia.	2003	12793308
is there an association between life events, postnatal depression and thyroid dysfunction in thyroid antibody positive women?	postnatal depression is more common in women positive for thyroid autoantibodies, independent of thyroid hormone dysfunction, but the basis of this association is unclear.	2003	12793517
thyroid hormone synthesis and storage in the thyroid gland of human neonates.	the aim of this study was to evaluate the morphological and biochemical maturation of the thyroid gland in human neonates. the mean iodine concentration in the thyroid gland of very premature infants (less than 32 weeks gestational age, 0-3 days survival, n = 12) was significantly lower than in the older group (34-41 weeks gestational age, 0-30 days survival; n = 15; p < 0.05). for the whole group of neonates there was a statistically significant linear correlation between duration of life, i.e. ...	2003	12793603
[two cases of intramedullary spinal cord metastasis of lung cancer detected with mri].	we report two cases of intramedullary spinal cord metastasis of lung cancer detected by mri. case 1: a 77-year-old man underwent chemotherapy and left lower lung lobectomy for squamous cell carcinoma of the lung (t2n0m0). about one year later, he complained of paresthesia of the lower extremities and claudication on walking, and then of weakness of the lower limbs and bladder dysfunction. magnetic resonance imaging (mri) revealed an enhanced mass in the dural sac at the level of the spines of l1 ...	2003	12795190
the endopolygalacturonase 1 from botrytis cinerea activates grapevine defense reactions unrelated to its enzymatic activity.	a purified glycoprotein from botrytis cinerea (strain t4), identified as endopolygalacturonase 1 (t4bcpg1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (vitis vinifera cv. gamay). these reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. most of these defense reactions were also activated in grapevine in response to ...	2003	12795381
five-year maintenance follow-up of early-onset periodontitis patients.	the purpose of this study was to evaluate the clinical and microbiological status of patients with early-onset or aggressive periodontitis (eop) who had received supportive periodontal care (spc) every 3-6 months for a period of 5 years, following active periodontal treatment.	2003	12795796
clinical characteristics of pneumonic-type adenocarcinoma of the lung.	to analyze diagnostic approaches, survival predictors, and treatment efficacy in pneumonic-type adenocarcinoma (p-adc).	2003	12796162
neoadjuvant doxil chemotherapy prior to androgen ablation plus radiotherapy for high-risk localized prostate cancer: feasibility and toxicity.	patients with clinical t3 or t4 prostate cancer or with elevated serum prostate-specific antigen (psa) levels greater than 40 ng/ml are at high risk of failure with primary treatment. chemotherapy administered at the time of diagnosis may decrease the risk of recurrence. patients with high risk localized prostate cancer were treated with two cycles of liposomal doxorubicin (doxil) at 50 mg/m2 every 28 days. patients were assessed for response by digital rectal examination (dre), psa, and endorec ...	2003	12796607
evaluation of rat thyroid gland morphophysiological status after three months exposure to 50 hz electromagnetic field.	objective of our study was to use morphophysiological criteria in order to determine the sensitivity of male rat thyroid gland to an extremely low frequency electromagnetic field (elf-emf) influence and the ability of the gland to repair after period of exposure. animals were exposed to 50hz, 50-500 microt elf-emf for 3 months when a part of them (group i) were sacrificed, while the rest of animals were subjected to recovery evaluation of the gland and sacrificed after 1 (group ii), 2 (group iii ...	2003	12798131
the diversity and evolution of the t4-type bacteriophages.	recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect eubacteria and archaea, constitute a substantial fraction of this population. in spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is known about them. for the last 10 years our research has focused on an extremely widespread group of phages, the t4-type. it has now become evident that phage t4 has a myriad of relat ...	2003	12798230
canonical nucleosides can be utilized by t4 dna ligase as universal template bases at ligation junctions.	t4 dna ligase catalyzes the template-dependent ligation of dna. using t4 dna ligase under specific experimental conditions, we demonstrate that each of the four canonical nucleosides, centrally located on a template molecule such that they flank the site of ligation, can direct the ligation of nucleic acids regardless of the identity of the terminal nucleosides being covalently joined. this universal templating capability extends to those positions adjacent to the ligation junction. this is the ...	2003	12799448
long-term results of intrathoracic chemohyperthermia (itch) for the treatment of pleural malignancies.	there is no standard treatment for patients with pleural malignancies. the aim of this prospective study was to investigate the toxicity and long-term results of a multimodality treatment consisting of surgery and intrathoracic chemohyperthermia (itch) for the treatment of patients with pleural malignancies. from january 1990 to august 2000, 24 patients with mesothelioma (n=17), fibrosarcoma (n=3), pleural adenocarcinoma (n=3) and thymoma (n=1) were included. the mesothelioma stages were t1 or t ...	2003	12799624
[measuring disability of patients with low-back pain--validation of a german version of the roland & morris disability questionnaire].	in this study three instruments measuring disability of patients with low-back pain are presented and evaluated: (1) the behinderungsfragebogen (rm) - a german version of the roland & morris disability questionnaire (rdq) (2) a numerical rating scale measuring disability in general and (2) eight numerical rating scales measuring specific dimensions of disability (standing, sitting, walking, driving a car, carrying light loads, carrying heavy loads, sleeping, and sexual intercourse).	2000	12800012
cyclooxygenase-2 expression and angiogenesis in colorectal cancer.	cyclooxygenase-2 is involved in a variety of important cellular functions, including cell growth and differentiation, cancer cell motility and invasion, angiogenesis and immune function. however, the role of cyclooxygenase-2 as an angiogenic factor in colorectal cancer tissue is still unclear. we investigated the relationship between cyclooxygenase-2 and angiogenesis by analyzing the expression of cyclooxygenase-2 in colorectal cancer tissue, as well as its association with vascular endothelial ...	2003	12800231
distribution and anti-hbv effects of antisense oligodeoxynucleotides conjugated to galactosylated poly-l-lysine.	to describe distribution of the phosphorothioated antisense oligodeoxynucleotides (ps-asodns) conjugated to galactosylated poly-l-lysine (gal-pll) in mice, and to observe their effects on expression of hbv gene in the 2.2.15 cells and transgenic mice.	2003	12800234
thyroxine stimulates transglutaminase activity in articular chondrocytes.	thyroid hormones induce features of the hypertrophic phenotype in mature articular chondrocytes as well as in growth plate chondrocytes. hypertrophic chondrocytes are responsible for extracellular matrix mineralization, with formation of bone mineral in growth plate cartilage and pathologic calcium crystals in aging articular cartilage. elevated activity levels of the two transglutaminase (tgase) enzymes (type ii tgase and factor xiiia (fxiiia)) have recently been described as additional feature ...	2003	12801486
the longitudinal course of two cases with cretinism diagnosed after adolescence.	in japan, mass screening tests on newborns for cretinism have been performed since 1984, cretinism is a very rare condition. we report the clinical course and complications of longitudinal thyroid hormone replacement therapy (liothyronine sodium: t3) of two women with cretinism and ectopic thyroid gland for the past 33 years until 2001. they were born in april 1951 (case 1) and in january 1952 (case 2). on admission in june 1968, they were 17 and 16 years old. they had short stature, mental reta ...	2003	12802380
tamoxifen: 28-day oral toxicity study in the rat based on the enhanced oecd test guideline 407 to detect endocrine effects.	the main objective of this 28-day oral gavage toxicity study in the rat was to investigate which of the current and/or additional parameters of the oecd test guideline 407 would reliably and sensitively detect the endocrine-mediated effects of the nonsteroidal antiestrogen tamoxifen. in addition, as this study was performed using two subgroups of five animals of each sex run concurrently, it enabled an assessment of the intralaboratory reproducibility while also assessing the potential value of ...	2003	12802581
assembly of stable human type i and iii collagen molecules from hydroxylated recombinant chains in the yeast pichia pastoris. effect of an engineered c-terminal oligomerization domain foldon.	the c-propeptides of the pro alpha chains of type i and type iii procollagens are believed to be essential for correct chain recognition and chain assembly in these molecules. we studied here whether the 30-kda c-propeptides of the human pc alpha 1(i), pc alpha 2(i), and pc alpha 1(iii) chains, i.e. pro alpha chains lacking their n-propeptides, can be replaced by foldon, a 29-amino acid sequence normally located at the c terminus of the polypeptide chains in the bacteriophage t4 fibritin. the al ...	2003	12805365
contemporary management of superior pulmonary sulcus (pancoast) lung tumors.	for more than three decades following the initial report by shaw et al. in 1961, the standard treatment of pancoast lung tumors consisted of induction radiotherapy followed by en bloc resection through a posterolateral thoracotomy. overall 5-year survival rates with this regime were typically 30 to 40%, with poor prognosis in patients with positive mediastinal lymph nodes, t4 involvement, or incomplete resection. during the past decade, advancements in surgical technique and adjuvant therapy hav ...	2003	12806248
management of high-risk localized prostate cancer: the integration of local and systemic therapy approaches.	using a combination of psa, gleason score, and clinical stage, it is possible to identify a group of patients with prostate cancer who have a high risk of relapse following initial treatment (e.g., radiotherapy or radical prostatectomy). for these patients, multi-modal therapy may result in improved outcomes. we reviewed published literature to identify methods to identify high-risk patients as well as options for adjuvant or neoadjuvant therapy to reduce risk of disease recurrence. at the prese ...	2003	12810212
iron deficiency anaemia in childhood and thyroid function.	studies in animals and adults have indicated iron deficiency anaemia to be associated with altered thyroid hormone metabolism. the aim of the present study was to determine the effect of iron deficiency anaemia on the thyroid function of young children. concentrations of thyroxine (t4) and triiodothyronine (t3), free thyroid hormones (ft4 and ft3), thyroxine binding globulin (tbg), and thyroid stimulating hormone (tsh) were measured in the basal state and in response to an intravenous bolus of t ...	2003	12810411
low serum total thyroxine and free triiodothyronine in patients with hepatic encephalopathy due to non-alcoholic cirrhosis.	we evaluated serum thyroid hormone levels in non-alcoholic cirrhotic patients with and without hepatic encephalopathy.	2003	12811678
[a study on introduction of chitinase gene and beta-1,3-glucanase gene into restorer line of dian-type hybrid rice (oryza sativa l.) and enhanced resistance to blast (magnaporthe grisea)].	plasmid pblgc containing chitinase gene from phaseolus limensis and beta-1,3-glucanase gene from nicotiana tabacum was bombarded into the restorer line "nan29" of dian-type hybrid rice (oryza sativa l. ssp. japonica) from yunnan province of south-west china. 93 regenerants were obtained from the calli that were resistant to g418 (100 to 150 mg/l) on nb medium. using beta-1,3 glucanase gene as the probe, 17 of the regenerants were identified to be transgenic lines by dot blotting and the foreign ...	2003	12812057
ligation-based synthesis of oligonucleotides with block structure.	we describe here a method for the synthesis of oligonucleotides with block structure (padlock probes, primers for multiplex polymerase chain reaction (pcr), and ligation-independent cloning), based on the ligation of presynthesized parts by t4 dna ligase. the advantages of this approach are: (i) suitability of the technology for any producer-from synthesis company to laboratory, (ii) high quality and adjustable scale of synthesis, and (iii) possibility of including any modified bases inexpensive ...	2003	12814636
complications associated with selective high-dose intraarterial cisplatin and concomitant radiation therapy for advanced head and neck cancer.	to describe the incidence and severity of complications associated with intraarterial cisplatin and concomitant radiation therapy for advanced head and neck cancer.	2003	12817041
[study on behavior of thyroid gland hormones in bydgoszcz province children and teenagers with simplex struma].	struma caused by iodine deficit is an endemic disorder in specified regions. the purpose of the study was a biochemical characteristic of struma simplex in children from bydgoszcz province and evalued correlation between laboratory exponents of thyroid gland function, size of the gland and kind of the struma. it was confirmed that neutral struma of children generally takes a course with euthyroidism. in children with struma were confirmed significant lower values of t4 and no significant higher ...	1998	12818108
[the thyroid function in children with viral meningitis].	thyroid function was investigated in children affected with viral meningitis caused by mumpsvirus or enteroviruses. serum or plasma levels of thyroid-stimulating hormone (tsh), triiodothyronine (t3), free triiodothyronine (ft3), thyroxine (t4) and free thyroxine (ft4) were measured twice in course of the disease: at admission and at recovery (day 10-14 from the onset of illness). the levels of hormones were measured by radioimmunoassay (ria) or by enzyme linked fluorescent assay (elfa). a decrea ...	1998	12818109
[the thyroid function in children with purulent meningitis].	purulent meningitis (bm) is a complex process and its outcome is largely influenced by host's response, both inflammatory or endocrine, to the infection. in this study thyroid function in children with bm was investigated by measuring serum or plasma levels of thyroid-stimulating hormone (tsh), triiodothyronine (t3), free triiodothyronine (ft3), thyroxine (t4) and free thyroxine (ft4) 3 times in course of the disease: at admission, after 24-48 hrs. of treatment and at recovery (day 10-14 from th ...	1998	12818110
[thyroid autoantibodies in children with recently diagnosed insulin-dependent diabetes mellitus].	119 newly diagnosed insulin dependent diabetes mellitus (iddm) children - 59 girls and 60 boys aged 2-16 years were examined for the presence of thyroid autoantibodies (ta), t3, t4 and tsh levels. estimate was repeated after 2 years with sonography of thyroid in children with the presence of ta. at onset of iddm positive ta were found in 38 (32%) diabetic children - in 20 boys and 18 girls. the highest frequency of ta was stated in children aged 10-13 years. one boy with high ta was identified t ...	2002	12818117
[assessment of thyrotropin concentrations in children with somatotropin deficiency treated with growth hormone].	there are contradictory literature opinions on the influence of gh therapy on the hypothalamus-hypophysis-thyroid gland axis concerning tsh secretion. some researchers describe the possibility of complete inhibition of tsh secretion followed by overt hypothyroidism, the others do not confirm it.	2002	12818126
a novel intraarterial chemotherapy using paclitaxel in albumin nanoparticles to treat advanced squamous cell carcinoma of the tongue: preliminary findings.	the purpose of our study was to evaluate the effectiveness of intraarterial infusion of paclitaxel incorporated into human albumin nanoparticles for use as induction chemotherapy before definitive treatment of advanced squamous cell carcinoma of the tongue.	2003	12818869
regeneration of ultraviolet-sensitive cones in the retinal cone mosaic of thyroxin-challenged post-juvenile rainbow trout (oncorhynchus mykiss).	previous studies in our laboratory have examined the loss of ultraviolet-sensitive (uvs) cones and uv sensitivity. this study looks at the question of regeneration of uvs cones and its topographic distribution, along with several other measures of the cone mosaic. topography of the cone mosaic in rainbow trout smolts (post-metamorphic juveniles) was examined under normal growth conditions and during an exogenous thyroid hormone (th) challenge. growth of trout retina was studied over six weeks. r ...	2003	12819272
dominant inhibition of thyroid hormone action selectively in the pituitary of thyroid hormone receptor-beta null mice abolishes the regulation of thyrotropin by thyroid hormone.	thyroid hormones, t4 and t3, regulate their own production by feedback inhibition of tsh and trh synthesis in the pituitary and hypothalamus when t3 binds to thyroid hormone receptors (trs) that interact with the promoters of the genes for the tsh subunit and trh. all tr isoforms are believed to be involved in the regulation of this endocrine axis, as evidenced by the massive dysregulation of tsh production in mice lacking all tr isoforms. however, the relative contributions of tr isoforms in th ...	2003	12819298
adenoid cystic carcinoma of salivary and lacrimal gland origin: localization, classification, clinical pathological correlation, treatment results and long-term follow-up control in 84 patients.	adenoid cystic carcinoma (acc) is a malignant tumor arising from glands. in the maxillofacial region, the salivary glands are particularly affected by acc and, less frequently, the lacrimal glands. the aim of this study was to determine the outcome of patients with acc in the maxillofacial region classified according to an internationally accepted staging system.	2003	12820326
monitoring of creatinine kinase during weaning of intrathecal baclofen and with symptoms of early withdrawal.	abrupt withdrawal from intrathecal baclofen (itb) can result in severe rebound spasticity, confusion, and seizures. it has been recently recognized that abrupt withdrawal from itb may, in rare cases, result in life-threatening rhabdomyolysis, hyperthermia, autonomic disturbances, and sepsis-like presentations. early recognition of the most severe forms of the withdrawal syndrome is essential for effective intervention. the common and unique features in such individuals seem to be severe increase ...	2003	12820794
characterization of the interactions between the bacteriophage t4 asia protein and rna polymerase.	the anti-sigma factor asia effects a change in promoter specificity of the escherichia coli rna polymerase via interactions with two conserved regions of the sigma(70) subunit, denoted 4.1 and 4.2. free asia is a symmetrical homodimer. here, we show that asia is monomeric when bound to sigma(70) and that a subset of the residues that contribute to the homodimer interface also contributes to the interface with sigma(70). asia interacts primarily with c-terminal sections of regions 4.1 and 4.2, wh ...	2003	12820881
serum 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) levels and thyroid function in air force veterans of the vietnam war.	we assessed potential health effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) concentration in serum on thyroid function in us air force veterans involved in operation ranch hand, the unit responsible for the aerial spraying of herbicides, including tcdd-contaminated agent orange, during the vietnam war from 1962 to 1971. other air force veterans who were not involved with spraying herbicides were included as comparisons.	2003	12821272
[influence of cardiopulmonary bypass (cpb) on the natriuretic peptides anp and bnp. a comparison between long and short duration of cpb and off pump surgery].	plasma atrial and brain natriuretic peptide (anp and bnp) are cardiac hormones secreted mainly due to cardiac overload. the study was designed to assess plasma concentrations of anp and bnp in patients undergoing coronary bypass grafting (cabg) with long and short duration of cardiopulmonary bypass (cpb) and without cpb (off-pump surgery).	2003	12822117
[construction of eukaryotic expression vector for rat myogenin gene].	to construct eukaryotic expression vector of rat myogenin gene for further study on its functions in skeletal muscle denervated atrophy and repair.	2003	12822357
survival and prognostic factors of surgically resected t4 non-small cell lung cancer.	category t4 nonsmall cell lung cancer (nsclc) encompasses heterogenous subgroups. we retrospectively analyzed the survival of patients with surgically resected t4 nsclc to evaluate the evidence for prognostic implications according to the subgroups of t4 category, nodal status, and resection completeness.	2003	12822610
small change in a g-rich sequence, a dramatic change in topology: new dimeric g-quadruplex folding motif with unique loop orientations.	nmr study has shown that dna oligonucleotide d(g(3)t(4)g(4)) adopts an asymmetric bimolecular g-quadruplex structure in solution. the structure of d(g(3)t(4)g(4))(2) is composed of three g-quartets, overhanging g11 residue and g3, which is part of the loop. unique structural feature of d(g(3)t(4)g(4))(2) fold is the orientation of the two loops. thymidine residues t4-t7 form a diagonal loop, whereas t15-t18 form an edge type loop. the g-quadruplex core of d(g(3)t(4)g(4))(2) consists of two stack ...	2003	12823005
resection surgery with neoadjuvant chemoradiotherapy improves outcomes of patients with t4 esophageal carcinoma.	the prognosis of patients with t4 esophageal carcinoma is poor, and thus an effective treatment needs to be established. the present study assessed the effect of chemoradiotherapy (crt), postoperative morbidity and mortality, and survival time in 41 patients with t4 esophageal carcinoma. of these, 24 received crt followed by surgery (group a) and the remaining 17 were treated with crt alone (group b). postoperative complications in group a were compared with 251 patients (group c) who underwent ...	2003	12823205
inflammatory infiltrate of chronic periradicular lesions: an immunohistochemical study.	to determine the cellular profile of human chronic periradicular lesions using immunohistochemical methods in order to study the differences in the cell infiltrate of periradicular granulomas and cysts.	2003	12823701
potential role for 53bp1 in dna end-joining repair through direct interaction with dna.	upon dna damage, p53-binding protein 1 (53bp1) relocalizes to sites of dna double-strand breaks and forms discrete nuclear foci, suggesting its role in dna damage responses. we show that 53bp1 changed its localization from the detergent soluble to insoluble fraction after treatment of cells with x-ray, but not with ultraviolet or hydroxyurea. either dnase or phosphatase treatment of the insoluble fraction released 53bp1 into the soluble fraction, showing that 53bp1 binds to chromatin in a phosph ...	2003	12824158
mineral toxicity and physiological functions in tree seedlings irrigated with effluents of varying chemistry in sandy soil of dry region.	effluents namely canal water as control (t1); municipal effluent (t2); textile effluent (t3); steel effluent (t4); textile + municipal effluent in 1:1 ratio (t5); steel + municipal effluent in 1:2 ratio (t6); steel + municipal + textile in 1:2:2 ratio (t7); and steel + textile in 1:2 ratio (t8) were added to the seedlings of acacia nilotica and eucalyptus camaldulensis. the aim was to monitor the toxic effect of the accumulated mineral ions on the physiological functions and ultimately to utiliz ...	2003	12826032
the number of injections does not influence local anesthetic absorption after paravertebral blockade.	the aims of this study are to determine if the injection of a single large dose of local anesthetics into the paravertebral space increases the risks of inducing toxicity compared with multiple small injections and to describe ropivacaine plasma concentrations resulting from paravertebral blockade.	2003	12826547
predictive factors in locally advanced rectal cancer treated with preoperative hyperfractionated and accelerated radiotherapy.	this study examines the prognostic significance of pathologic factors in patients with primary locally advanced rectal cancer treated prospectively with preoperative radiotherapy. from 1992 to 1998, 104 patients with rectal cancer of grades t3 or t4 and any n underwent preoperative radiotherapy followed by surgical resection. survival curves were estimated according to the kaplan-meier method. correlation of outcome with clinicopathologic variables (pathologic tumor and lymph node staging, histo ...	2003	12827607
metabolic rate and its relationship with ascites in chicken genotypes.	1. male chickens of 7 genetic lines, consisting of two pure sire and two pure dam populations, a commercial broiler cross and two slow-growing chicken lines (label rouge, lr and mechelse koekoek, mk) were reared from 1 to 37 d of age. the chickens received a two-phase dietary regime and were subjected to low ambient temperature. 2. the experimental aim was to investigate relationships between susceptibility to ascites and heat production. 3. body weight gain (bwg), food intake and food conversio ...	2003	12828217
transverse myelitis secondary to coexistent lyme disease and babesiosis.	to describe transverse myelitis secondary to coexistent lyme disease and babesiosis.	2003	12828297
improved local control with stereotactic radiosurgical boost in patients with nasopharyngeal carcinoma.	treatment of nasopharyngeal carcinoma using conventional external beam radiotherapy (ebrt) alone is associated with a significant risk of local recurrence. stereotactic radiosurgery (str) was used to boost the tumor site after ebrt to improve local control.	2003	12829140
resection of bronchopulmonary cancers invading the left atrium--benefit of cardiopulmonary bypass.	cardiopulmonary bypass (cpb) for extended lung resections involving great vessels and other mediastinal organs remains controversial, especially due to cpb-related haemorrhagic and immunological issues. here, we will retrospectively analyse the results obtained with such procedure.	2003	12833206