Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms.human beta-defensins 2 and 3 (hbd-2 and hbd-3) are inducible peptides present at sites of infection in the oral cavity. a few studies have reported broad-spectrum antimicrobial activity for both peptides. however, no comprehensive study has thoroughly investigated their potential against oral pathogens. the purpose of this study was to test the effectiveness of hbd-2 and hbd-3 against a collection of oral organisms (actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gin ...200415004048
influence of resin monomers on growth of oral streptococci.ethyleneglycol dimethacrylate monomers have been previously reported to stimulate the growth of certain caries-associated bacteria on the basis of turbidity measurements. to elucidate the detail of this effect, we examined the influence of resin monomers on the growth of streptococcus sobrinus and streptococcus sanguis by determination of bacterial numbers (colony-forming units), morphological observation, and chemical analysis. although the absorbance values in the stationary phase of bacterial ...200415044503
effect of a separated instrument on bacterial penetration of obturated root canals.the aim of this study was to determine the effect a separated instrument has on the time required for bacterial penetration of obturated root canals. twenty-six extracted human mandibular premolars with single canals were used in the study. group 1 consisted of teeth that contained a separated size 40 profile rotary file and were obturated with gutta-percha and zinc oxide eugenol sealer to the level of the separated file. group 2 consisted of teeth that were similarly obturated, but without a se ...200415055438
[dynamic observation of periodontal pathogens and cariogenic bacteria in modified chemostat using confocal laser scanning microscopy].to study dynamic relation between periodontal pathogens and cariogenic bacteria under analogous oral environment.200415061891
lactoferrampin: a novel antimicrobial peptide in the n1-domain of bovine lactoferrin.the antimicrobial activity of bovine lactoferrin is attributed to lactoferricin, situated in the n1-domain. based on common features of antimicrobial peptides, a second putative antimicrobial domain was identified in the n1-domain of lactoferrin, designated lactoferrampin. this novel peptide exhibited candidacidal activity, which was substantially higher than the activity of lactoferrin. furthermore, lactoferrampin was active against bacillus subtilis, escherichia coli, and pseudomonas aeruginos ...200415062998
preliminary evaluation of bioactive glass s53p4 as an endodontic medication in vitro.calcium hydroxide is the "gold standard" endodontic medication, but it may fail to eliminate certain facultative bacteria and yeasts found in root canal systems. in this study, standardized bovine dentin blocks infected with enterococcus faecalis were treated with an aqueous calcium hydroxide or bioactive glass s53p4 (bag) powder suspension. while calcium hydroxide was ineffective, the bag suspension eliminated the infection in the sampled dentin layers after 5 days. in a direct exposure test, p ...200415085050
selection for high-level resistance by chronic triclosan exposure is not universal.to investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria.200415117935
salivary receptors for the proline-rich protein-binding and lectin-like adhesins of oral actinomyces and streptococci.colonization of the tooth surface by actinomyces and viridans group streptococci involves the attachment of these bacteria to adsorbed salivary components of the acquired enamel pellicle. the hypothesis that this attachment depends on specific adhesins has now been assessed from the binding of bacteria with well-defined adhesive properties to blots of sds-page-separated parotid and submandibular-sublingual (sm-sl) saliva. streptococcus sanguis and type 2 fimbriated actinomyces naeslundii, which ...200415153461
[a comparative analysis of biological properties of glucosylrtanterases secreted by streptococcus sanguis and streptococcus mutans]our study make a comparative analysis of biological properties of glucosyltranterases(gtf) secreted by s.sanguis 34 and s.mutans ingbritt(c).the results as follows:(1)s.sanguis 34 has more extractions of extracelluar gtf than s.mutans ingbritt(c) under equal cultured conditions,but s.mutans inggbritt(c) has more activity of extracelluar gtf than s.sanguis.these differences are identical with the difference of their cariogenicities;(2)gtf of s.sanguis 34 can also synthesize water-soluble glucan,a ...199615159987
[environmental factors in oral cavity effect on growing of streptococcus sanguis and streptococcus mutans-effect of mucin]our study use continues culture technique through adding mucin as the limiting element in chemically defined culture,medium in order to observe growing states of s.sanguis 34 and,s.mutants ingbritt(c).the results as follows:s.sanguis 34 were able to grow as pure culture on mucin;s.mutans was unable to grow as a pure culture on mucin,but attained a significant population size in the presence of s.sanguis.these results indicate a synergetic degradation of mucin by s.sanguis and s.mutans in mixed c ...199615159988
[environmental factors in oral cavity effect on growing of streptococcus sanguis and strepococcus mutans-oxygen of effects]oral streptococcus species belong to facultative aerobes,and are easily inhibited by metabolic products of oxygen.our study uses continues culture technique through adding oxygen in order to observe growing states of s.sanguis 34 and s.mutans ingbritt(c) the result is that the resistance to oxygen of s.sanguis 34 is stronger than s.mutans ingbritt(c).this result is in accordance with the phenomenon that s.sanguis is a pioneer colonizing germ of dental plaque.the lower resistance to oxygen of s.m ...199615159989
the effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored proroot mineral trioxide aggregate.the purpose of this in vitro study was to determine whether the substitution of 0.12% chlorhexidine gluconate for sterile water as a mixing agent would enhance the antimicrobial activity of tooth-colored proroot mineral trioxide aggregate (mta) against actinomyces odontolyticus (atcc17982), fusobacterium nucleatum (atcc2586), streptococcus sanguis (atcc10556), enterococcus faecalis (er3/2s), escherichia coli (sm10lambdapir), staphylococcus aureus (atcc6538), pseudomonas aeruginosa (ume), and can ...200415167473
the bacteriology of the supragingival plaque of child dental patients in kuwait.to investigate the bacterial flora of the gingival plaques of pediatric dental patients in order to establish baseline data for future studies on the complex microbiota of the oral cavity of this group of child dental patients.200415181322
a novel glucan-binding protein with lipase activity from the oral pathogen streptococcus mutans.streptococcus mutans produces extracellular glucosyltransferases (gtfs) that synthesize glucans from sucrose. these glucans are important in determining the permeability properties and adhesiveness of dental plaque. gtfs and the gbpa glucan-binding protein are characterized by a binding domain containing a series of 33-amino-acid repeats, called 'a' repeats. the s. mutans genome sequence was searched for orfs containing 'a' repeats, and one novel gene, gbpd, which appears to be unique to the mut ...200415184580
[analysis of oral microflora early colonized in infants].to observe the dynamic changes of oral microflora early colonized in infants.200415190795
infective endocarditis in patients with liver cirrhosis.infective endocarditis complicating liver cirrhosis is infrequently reported. this study evaluated the clinical features of infective endocarditis in taiwanese patients with liver cirrhosis.200415216401
molecular interactions of surface protein peptides of streptococcus gordonii with human salivary components.oral streptococci play a large role in dental biofilm formation, and several types interact as early colonizers with the enamel salivary pellicle to form the primary biofilm, as well as to incorporate other bacteria on tooth surfaces. interactions of surface molecules of individual streptococci with the salivary pellicle on the tooth surface have an influence on the etiological properties of an oral biofilm. to elucidate the molecular interactions of streptococci with salivary components, bindin ...200415271944
[hydropneumothorax in an immunocompetent patient]. 200415283647
[purification and characterization of sanguicin--a bacteriocin produced by streptococcus sanguis].streptococcus sanguis plays an important role in maintaining the periodontal microecological balance. previous studies have demonstrated that sanguicin--a kind of bacteriocin produced by s. sanguis has a prominent function of inhibiting the growth of putative periodontopathic bacteria (ppb). the aim of this study was to purify sanguicin and investigate its characters.200415291127
effects of mouthrinses with triclosan, zinc ions, copolymer, and sodium lauryl sulphate combined with fluoride on acid formation by dental plaque in vivo.bacteriological tests demonstrated a slight synergistic effect of triclosan and sodium lauryl sulphate (sls) on the growth of streptococcus mutans nctc 10449 and streptococcus sanguis atcc 10556 in vitro. a single mouthrinse with sls (17.4 mm) or sls plus triclosan (3.5 mm) significantly decreased the number of salivary mutans streptococci in a group of 12 subjects up to 90 min after rinsing. the effect on plaque ph of a mouthrinse with either 12.0 mm naf, naf plus 10.0 mm zinc acetate, naf plus ...200415316186
in vitro evaluation of the antimicrobial activity of five root canal sealers.the aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: endo fill, endomethasone, endomethasone n, sealer 26 and ah-plus, against the following microorganisms: candida albicans, staphylococcus aureus, enterococcus faecalis, streptococcus sanguis and actinomyces naeslundii. the sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.the direct contact method through the observation of the microbial growth in liquid medium and the agar ...200415322642
a simple approach to examine early oral microbial biofilm formation and the effects of treatments.a simple in vivo approach to examine early dental plaque formation in the human mouth and to determine the effects of common dietary and oral hygiene procedures on biofilm formation is reported.200415327641
bactericidal effects of a fusion protein of llama heavy-chain antibodies coupled to glucose oxidase on oral bacteria.enzymes such as lactoperoxidase and glucose oxidase (gox) are used as antimicrobial agents in oral care products. their low specificities and substantiveness can be reduced by covalent coupling of antimicrobial molecules to antibodies. variable domains (v(hh)) derived from llama heavy-chain antibodies are particularly suited for such an approach. the antibodies are composed solely of heavy-chain dimers; therefore, production of active fusion proteins by using molecular biology-based techniques i ...200415328101
[oral streptococci: factors and mechanisms of pathogenicity (part ii)]. 200315341321
an in-vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine.to determine whether nisin, a bacteriocin, would be effective at killing enterococcus faecalis and streptococcus gordonii cells in solution and within the root canal system.200415347290
adherent bacteria cells in five dental materials: sonication effect.adherent bacterial cells on the surfaces of two dental porcelain ceramics, three composite resins and human enamel were examined using four types of bacteria strains. their adherent cells were counted on saliva-coated and uncoated material surfaces after sonication, and contact angle and zeta potential were measured for each adherent cell tested. a correlation between contact angle and bacterial cells on an uncoated surface was found to be higher in two streptococcus sanguis cells than in s. mut ...199815348918
the antimicrobial potential of 14 natural herbal dentifrices: results of an in vitro diffusion method study.increasing numbers of americans are using natural herbal products for general and oral health care. few of these products, however, have undergone rigorous testing, as evidenced by the limited amount of information on their safety and efficacy in the literature. the authors conducted an in vitro study to evaluate the antimicrobial potential of 14 natural herbal dentifrices.200415387052
[streptococcus sanguis white in subacute bacterial endocarditis]. 195015441351
path-dependency of the interaction between coaggregating and between non-coaggregating oral bacterial pairs--a thermodynamic approach.coaggregation, i.e. specific recognition between bacteria from different species, is a well-described phenomenon in the human oral cavity but remains physically poorly understood. with our study we aimed at elucidating some aspects of the mechanism of the coaggregation between the oral bacteria streptococcus oralis j22 and actinomyces naeslundii 147, in particular with respect to the driving force for coaggregation and its pathway-dependency. to that end, the macroscopic turbidity of the bacteri ...200415450309
oral streptococcal glyceraldehyde-3-phosphate dehydrogenase mediates interaction with porphyromonas gingivalis fimbriae.interaction of porphyromonas gingivalis with plaque-forming bacteria is necessary for its colonization in periodontal pockets. participation of streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase (gapdh) and p. gingivalis fimbriae in this interaction has been reported. in this investigation, the contribution of various oral streptococcal gapdhs to interaction with p. gingivalis fimbriae was examined. streptococcal cell surface gapdh activity was measured by incubation of a constant num ...200415488735
streptococcus sinensis may react with lancefield group f antiserum.lancefield group f streptococci have been found almost exclusively as members of the 'streptococcus milleri' group, although they have been reported very occasionally in some other streptococcal species. among 302 patients with bacteraemia caused by viridans streptococci over a 6-year period, three cases were caused by streptococcus sinensis (type strain hku4t, hku5 and hku6). all three patients had infective endocarditis complicating their underlying chronic rheumatic heart diseases. gene seque ...200415496384
synergistic effects of streptococcal glucosyltransferases on adhesive biofilm formation.glucosyltransferases (gtf)-i and gtf-si of streptococcus mutans synthesize water-insoluble and both water-soluble and -insoluble glucans, respectively, and play essential roles in the sucrose-dependent adhesion of the organism to tooth surfaces. to examine the interactions of different gtfs on artificial biofilm formed by s. mutans and other oral streptococci, we generated gtf-i- and gtf-si-hyperproducing isogenic mutant strains. transformant b42-21, which hyperexpressed gtf-si, exhibited firm a ...200415505239
[periodontitis and cardiovascular diseases--review of publications].nowadays periodontal diseases are treated as a one of a social diseases. the main consequences of them are: premature teeth loss and possibility of inducing, aggravating and modifying many systemic disorders, such as endo- and myocarditis, glomerulonephritis, iriditis, retinitis, rheumatic polyarthritis. the scientific data performed in the last 10 years indicate links between periodontitis and atheromatosis, coronary heart disease and acute coronary events, including myocardial infarction. in t ...200415515818
formation of trimethylamine from dietary choline by streptococcus sanguis i, which colonizes the mouth.choline is a component of the normal diet, and when humans ingest large amounts they excrete trimethylamine (which can impart a fishy body odor). in the presence of nitrite, trimethylamine can be converted to dimethylnitrosamine, a potent carcinogen. bacteria in the large intestine metabolize choline to form trimethylamine. we determined that a bacterium normally present in the oral cavity also has this capacity. mixed bacterial flora cultured from dental plaque and saliva converted choline to t ...199015539190
surface free energy and bacterial retention to saliva-coated dental implant materials--an in vitro study.the aim of the present investigation was to compare the in vitro bacterial retention on saliva-coated implant materials (pure titanium grade 2 (cp-ti) and a titanium alloy (ti-6al-4v) surfaces), presenting similar surface roughness, and to assess the influence of physico-chemical surface properties of bacterial strain and implant materials on in vitro bacterial adherence. two bacterial strains (one hydrophilic strain and one hydrophobic strain) were used and the following were evaluated: bacteri ...200415555904
single species biofilm-forming ability of root canal isolates on gutta-percha points.the participation of bacterial biofilms in the over-filled gutta-percha points associated with refractory periapical periodontitis has recently been reported. this study investigated the initial biofilm-forming ability of root canal isolates (enterococcus faecalis, streptococcus sanguis, strep. intermedius, strep. pyogenes, staphylococcus aureus, fusobacterium nucleatum, propionibacterium acnes, porphyromonas gingivalis and prevotella intermedia) on gutta-percha points in vitro. each bacterial s ...200415560836
measurement of bacterial concentration fractions in polymicrobial mixtures by raman microspectroscopy.relative concentrations of streptococcus mutans and streptococcus sanguis are important parameters in the study of dental caries, but current methods of measuring these concentrations are time consuming and prone to inaccuracies. we investigate the use of raman spectroscopy for measuring relative concentrations of these two bacterial species in solid mixtures. to our knowledge, this is the first time raman spectroscopy has been used to analyze bacterial mixtures rather than to identify the speci ...200415568938
behcet's disease.behcet's disease (bd) is a systemic disorder of recurrent acute inflammation, characterized by major symptoms of oral aphthous ulcers, uveitis, skin lesions and genital ulcers. involvement of intestines, vessels, and central nervous system (cns) sometimes leads to a poor prognosis. patients with bd are known to distribute along the ancient silk road. the incidence is relatively higher from eastern asia to the mediterranean area as roughly 1-10 patients in 10,000 people, whereas only 1-2 patients ...200415598081
a comparison of decontamination methods used for dental burs.this study investigated the bacterial and fungal contamination of used dental burs. a novel assay system for comparison of efficacy of pre-sterilisation cleaning techniques for dental burs was used to evaluate manual scrubbing, enzymic agents and washer-disinfectors.200415611751
differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease.previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease.200515642056
[treatment of periimplantitis with laser or ultrasound. a review of the literature].in addition to conventional treatment modalities (mechanical and chemical), the use of different lasers has been increasingly proposed for the treatment of peri-implantitis. results from both controlled clinical and basic studies have pointed to the high potential of an er:yag-laser. its excellent ability to effectively ablate dental calculus without producing major thermal side-effects to adjacent tissue has been demonstrated in numerous studies. recently, a new ultrasonic device has been used ...200415646670
swabbing of waiting room magazines reveals only low levels of bacterial contamination.previous studies have shown that toys in waiting rooms of general practice surgeries can be contaminated with potentially pathogenic bacteria. the question was raised as to whether magazines might also be sources of contamination. swabbing of the front page of 15 magazines from 11 general practice surgeries, followed by analysis for total and specific bacteria, revealed low levels of contamination. among targeted groups of pathogens only two colonies of staphylococcus aureus were detected. magaz ...200515667764
neutrophil and lymphocyte responses to oral streptococcus in adamantiades-behçet's disease.immune reactions against microorganisms play an important pathogenic role in adamantiades-behçet's disease. we had previously obtained streptococcus sanguinis (strain bd113-20), isolated from the oral cavity of patients with adamantiades-behçet's syndrome. to investigate the pathogenesis of this isolate, we examined neutrophil reactions and levels of cytokine production by lymphocytes after stimulation with the strain. the reactions of neutrophils were examined by chemiluminescence assay using w ...200515681141
cytochemical differences in bacterial glycocalyx.to examine new cytochemical aspects of the bacterial adhesion, a strain 41452/01 of the oral commensal streptococcus sanguis and a wild strain of staphylococcus aureus were grown with and without sucrose supplementation for 6 days. osmiumtetraoxyde (oso4), uranyl acetate (ua), ruthenium red (rr), cupromeronic blue (cb) staining with critical electrolytic concentrations (cecs), and the tannic acid-metal salt technique (tamst) were applied for electron microscopy. cytochemically, only rr-positive ...200515683566
effect of mutations in the human immunoglobulin a1 (iga1) hinge on its susceptibility to cleavage by diverse bacterial iga1 proteases.components of the human immunoglobulin a1 (iga1) hinge governing sensitivity to cleavage by bacterial iga1 proteases were investigated. recombinant antibodies with distinct hinge mutations were constructed from a hybrid comprised of human iga2 bearing half of the human iga1 hinge region. this hybrid antibody and all the mutant antibodies derived from it were resistant to cleavage by the iga1 proteases from streptococcus oralis and streptococcus mitis biovar 1 strains but were cleaved to various ...200515731049
influence of titanium surface roughness on attachment of streptococcus sanguis: an in vitro study.the purpose of the present study was to investigate the efficacy of the decontamination protocol for bacterial removal in titanium surfaces with three different levels of roughness using a high-pressure sodium bicarbonate device for 1 minute under aseptic conditions. group 1 was composed of 10 as-machined titanium sheets and groups 2 and 3 of titanium sheets blasted with aluminum oxide (al2o3, alumina) particles with different diameters: group 2 was blasted with 65-microm particles and group 3 w ...200515764951
[bacterial endocarditis in an adult on the interventricular septal congenital defect]. 200515777128
a serine-rich glycoprotein of streptococcus sanguis mediates adhesion to platelets via gpib.streptococcus sanguis is the most common oral bacterium causing infective endocarditis and its ability to adhere to platelets, leading to their activation and aggregation, is thought to be an important virulent factor. previous work has shown that s. sanguis can bind directly to platelet glycoprotein (gp) ib but the nature of the adhesin was unknown. here, we have shown that a high molecular weight glycoprotein of s. sanguis mediates adhesion to glycocalacin. the bacterial glycoprotein was purif ...200515801962
[the effect of galla chinensis on the growth of cariogenic bacteria in vitro].the purples of this study was to investigate the role of different components of galla chinensis extract on the growth of 6 kinds of cariogenic bacteria, and to find out the most effective components of galla chinensis extract.200515804032
scaling and root planing, systemic metronidazole and professional plaque removal in the treatment of chronic periodontitis in a brazilian population ii--microbiological results.the current investigation evaluated changes in levels and proportions of 39 bacterial species in subgingival plaque samples after scaling and root planing (srp) alone or in combination with systemic metronidazole and/or professional cleaning in subjects with chronic periodontitis.200515811059
mutanolysin, bacteriolytic agent for cariogenic streptococci: partial purification and properties.mutanolysin partially purified from the culture filtrate of streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near ph 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. mutanolysin exhibited maximal lytic activity at 60 c in the ph range 6.5 to 7.0 and was stable at 50 c in the acid range. n-bromosuccinimide caused complete inhibition of lytic activity at 1 mm, whereas calcium and magnesium ions at the same concen ...197415828186
mitral valve "kissing" vegetation. 200515831628
[prognostic factors of clinical outcome of the tuberculous pleural effusion].to date, no studies have been reported analyzing the prognosis factors related to severe clinical complications in tuberculous pleural effusion (tpe). we studied clinical, biochemical, radiological, and microbiological factors that could help establish the clinical outcome of tpe.200515847766
susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells.antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated.200515886266
streptococcus sanguis-induced platelet activation involves two waves of tyrosine phosphorylation mediated by fcgammariia and alphaiibbeta3.the low-affinity igg receptor, fcgammariia, has been implicated in streptococcus sanguis-induced platelet aggregation. therefore, it is likely that signal transduction is at least partly mediated by fcgammariia activation and a tyrosine kinase-dependent pathway. in this study the signal transduction mechanisms associated with platelet activation in response to the oral bacterium, s. sanguis were characterised. in the presence of igg, s. sanguis strain 2017-78 caused the tyrosine phosphorylation ...200515886812
molecular technique identifies the pathogen responsible for culture negative infective endocarditis.a case of culture negative endocarditis complicated by immune complex glomerulonephritis and severe aortic regurgitation necessitated aortic valve replacement. empirical treatment with penicillin and gentamicin according to uk guidelines was started. the pathogen, streptococcus sanguis, was later identified by polymerase chain reaction amplification and sequencing of bacterial 16s ribosomal rna. this molecular technique is likely to be of increasing importance in determining the aetiology of cul ...200515894748
comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens.the purpose of this study was to compare the antimicrobial activities of 6% and 3% sodium hypochlorite (naocl), 2% and 0.12% chlorhexidine gluconate (chx), and 0.01% and 0.005% doxycycline (doxy) on four microorganisms associated with primary endodontic infections. the agar diffusion test was used to measure antimicrobial activities of these agents against peptostreptococcus micros, prevotella intermedia, streptococcus sanguis, and lactobacillus acidophilus. minimum inhibitory concentration anal ...200515917691
microcalorimetric study on the influence of temperature on bacterial coaggregation.binding isotherms and heats of interaction have been determined at 15, 25, and 40 degrees c for a coaggregating and a non-coaggregating oral bacterial pair. heats of interaction were measured upon three consecutive injections of streptococci into an actinomyces suspension using isothermal titration calorimetry. after each injection, the number of streptococci injected remaining free in suspension was quantified microscopically and the degree of binding between the two bacterial strains was estab ...200515925611
[an in vitro study on effect of nidus vespae extract on the acid production of three strains of oral bacteria].to inquire into the effect of different of nidus vespae extract (nve) on growth and acid production of streptococcus mutans, streptococcus sanguis and actinomyces viscosus.200515931873
bacterial biofilm development on hydroxyapatite-coated glass.glass plates are frequently used as the substratum in flow cell experiments to allow continuous non-destructive observations of biofilm development via microscopy. the aim of this study was to evaluate hydroxyapatite-coated glass as a substratum for flow cell experiments, in comparison to plain glass, for modelling primary colonization of the tooth surface by streptococcus sanguis. glass plates were magnetron sputter coated with hydroxyapatite, producing a thin transparent layer. biofilm develop ...200515942698
the influences of hinge length and composition on the susceptibility of human iga to cleavage by diverse bacterial iga1 proteases.the influences of iga hinge length and composition on its susceptibility to cleavage by bacterial iga1 proteases were examined using a panel of iga hinge mutants. the iga1 proteases of streptococcus pneumoniae, streptococcus sanguis strains sk4 and sk49, neisseria meningitidis, neisseria gonorrhoeae, and haemophilus influenzae cleaved iga2-iga1 half hinge, an ab featuring half of the iga1 hinge incorporated into the equivalent site in iga1 protease-resistant iga2, whereas those of streptococcus ...200515944283
[behçet's disease]. 200515954379
multi-target antimicrobial actions of zinc against oral anaerobes.zinc is used in oral care products as an antiplaque/antigingivitis agent. our objective was to assess the antimicrobial actions of zinc against oral anaerobes associated with gingivitis, specifically fusobacterium nucleatum and prevotella intermedia, with focus on catabolism and oxidative metabolism.200515958206
binding of porcine ficolin-alpha to lipopolysaccharides from gram-negative bacteria and lipoteichoic acids from gram-positive bacteria.protein(s) reactive with n-acetyl-d-glucosamine (glcnac) was isolated from porcine nonimmune serum. the molecular weight of the purified protein was found to be mainly 40 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. the n-terminal 10 amino acid sequence of the purified protein were found to be identical to that of porcine ficolin-alpha reported previously. in enzyme-linked immunosorbent assay, the purified protein was found to react with lipopolysac ...200615964070
mycotic aneurysm of the celiac trunk: from early ct sign to rupture.we present a case of the rapid development and rupture of a mycotic celiac trunk aneurysm. initially on multislice computed tomography (ms-ct) there was a normal celiac trunk with minimal haziness of the surrounding fat. only 2 weeks later the patient went into hypovolemic shock due to a ruptured celiac aneurysm. although aneurysms of the visceral arteries are rare, they are of major clinical importance as they carry a life-threatening risk of rupture. this case illustrates the use of ms-ct in d ...200515999241
repeat infective endocarditis: differentiating relapse from reinfection.repeat infective endocarditis due to the same species can represent relapse of the initial infection or a new infection. we used time-based clinical criteria and pulsed-field gel electrophoresis-based molecular criteria to classify 13 cases of repeat infective endocarditis as either relapse or reinfection. the agreement between clinical and molecular criteria was imperfect (agreement in 10 [77%] of 13 cases).200516007540
differentiation of mutans streptococci by intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.it is difficult to distinguish mutans streptococci on the species level, and even more so on the subspecies level. intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) (icm) was applied to reference strains of five of the species of the mutans group (streptococcus criceti, streptococcus downei, streptococcus mutans, streptococcus ratti, streptococcus sobrinus), nonmutans streptococci (streptococcus oralis, streptococcus mitis, streptococcus sali ...200516101961
a synergistic chlorhexidine/chitosan combination for improved antiplaque strategies.the minor efficacy of chlorhexidine (chx) on other cariogenic bacteria than mutans streptococci such as streptococcus sanguinis may contribute to uneffective antiplaque strategies.200516105089
identification of virulence determinants for endocarditis in streptococcus sanguinis by signature-tagged mutagenesis.streptococcus sanguinis is a gram-positive, facultative anaerobe and a normal inhabitant of the human oral cavity. it is also one of the most common agents of infective endocarditis, a serious endovascular infection. to identify virulence factors for infective endocarditis, signature-tagged mutagenesis (stm) was applied to the sk36 strain of s. sanguinis, whose genome is being sequenced. stm allows the large-scale creation, in vivo screening, and recovery of a series of mutants with altered viru ...200516113327
an improved arbitrary primed pcr method for rapid characterization of transposon insertion sites.modifications were made to published arbitrary primed polymerase chain reaction (ap-pcr) procedures that resulted in increased specificity and sensitivity. several arbitrary primer sequences were also evaluated, resulting in recommendations for primer design.200516157212
a laboratory model biofilm fermenter: design and initial trial on a single species biofilm.the minimum inhibitory concentration (mic) does not provide information on the efficacy of antimicrobial agents against infections involving biofilms, which are many times more resistant than planktonic forms of bacteria. this report is on the design and initial trial of a device for growing standard biofilms and testing antimicrobial agents.200516171430
post-traumatic endophthalmitis following penetrating injury with dental needle. 200616179935
disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite.to evaluate the effectiveness of chlorhexidine (chx) or sodium hypochlorite (naocl) in disinfecting gutta-percha cones, to verify contamination of gutta-percha cones in their boxes, and to identify microorganisms after intentional contamination by handling cones.200516182174
estimation of salivary bacteria capable of inhibiting and stimulating streptococcus mutans and its correlation to dental caries and untreated carious teeth.bacteriocins are bacteriocidal proteinaceous molecules produced by the gram-positive bacteria not active against the produced strain. many investigations have revealed that certain bacteria using antibacterial or the inhibitory substance inhibit some other bacteria. a study was conducted in a group of 60 children to ascertain whether any correlation exists between the proportion of salivary bacteria inhibiting and stimulating streptococcus mutans and the oral health indices (dmft, deft and commu ...200516224131
competition and coexistence between streptococcus mutans and streptococcus sanguinis in the dental biofilm.the human mucosal surface is colonized by the indigenous microflora, which normally maintains an ecological balance among different species. certain environmental or biological factors, however, may trigger disruption of this balance, leading to microbial diseases. in this study, we used two oral bacterial species, streptococcus mutans and streptococcus sanguinis (formerly s. sanguis), as a model to probe the possible mechanisms of competition/coexistence between different species which occupy t ...200516237003
microbial risk indicators of early childhood caries.the aim of this study was to use molecular identification methods, such as 16s rna gene sequence and reverse-capture checkerboard hybridization, for identification of the bacteria associated with dental caries and with dental health in a subset of 204 twins aged 1.5 to 7 years old. a total of 448 plaque samples (118 collected from caries-free subjects and 330 from caries-active subjects) were used for analysis. we compared the bacteria found in biofilms of children exhibiting severe dental carie ...200516272513
behçet's disease patients present high levels of deglycosylated anti-lipoteichoic acid igg and high il-8 production after lipoteichoic acid stimulation.lipoteichoic acid (lta), induces some of the clinical symptoms of behçet's disease (bd) in a rat animal model. these results led to the hypothesis that lta may also trigger bd in humans. we investigated the humoral and cellular immune response against lta and lipopolysaccharide (lps) in patients with bd, and compared these responses with those of patients with active chronic oral ulcers (ou) and normal controls.200516273761
oral streptococci and cardiovascular disease: searching for the platelet aggregation-associated protein gene and mechanisms of streptococcus sanguis-induced thrombosis.pathogenic mechanisms in infective endocarditis, disseminated intravascular coagulation, and cardiovascular events involve the aggregation of platelets into thrombi. attendant infection by oral bacteria contributes to these diseases. we have been studying how certain oral streptococci induce platelet aggregation in vitro and in vivo. streptococcus sanguis expresses a platelet aggregation-associated protein (paap), which contributes little to adhesion to platelets. when specific antibodies or pep ...200516277582
[comparison of galla chinesis and sodium fluoride on growth and metabolism of bacteria in biofilm model].to compare the antibacterial effect between sodium fluoride and galla chinesis in bioflim model. to evaluate the feasibility of application of gala chinesis in the clinical practice of caries prevention.200516285535
[the effect of sodium doecyl sulfate on streptococcus sanguis biofilm].to investigate the effects of sodium doecyl sulfate on streptococcus sanguis biofilm.200516288332
inability of probiotic bacterial strains lactobacillus rhamnosus hn001 and bifidobacterium lactis hn019 to induce human platelet aggregation in vitro.platelet aggregation contributes to the pathogenesis of infective endocarditis, and aggregation of platelets induced by lactobacilli is thought to be an important contributory factor in the development and progression of lactobacillus endocarditis. the main purpose of this study was to examine the effect of immunity-enhancing probiotic strains lactobacillus rhamnosus hn001 and bifidobacterium lactis hn019 on the activation and aggregation of human blood platelets. whole blood samples from health ...200516300090
effect of antimicrobial agents on the oral bacteria coaggregation. 198916312282
acute septic arthritis due to streptococcus sanguis.to present a case of acute septic arthritis due to streptococcussanguis, a member of the viridans group streptococci.200616340233
development of high-frequency delivery system for transposon tn919 in lactic streptococci: random insertion in streptococcus lactis subsp. diacetylactis 18-16.the conjugative transposon tn919, originally isolated in streptococcus sanguis fc1, is capable of low-frequency transfer (10 and 10 per recipient) on membrane filters to a wide number of streptococcal recipients including the industrially important lactic streptococci. the introduction of pmg600 (lac lax; a lactose plasmid capable of conjugative transfer at high frequencies and which, in certain hosts, confers an unusual clumping phenotype) into a streptococcus lactis ch919 donor, generating s. ...198716347268
early biofilm formation and the effects of antimicrobial agents on orthodontic bonding materials in a parallel plate flow chamber.decalcification is a commonly recognized complication of orthodontic treatment with fixed appliances. a technology, based on a parallel plate flow chamber, was developed to investigate early biofilm formation of a strain of streptococcus sanguis on the surface of four orthodontic bonding materials: glass ionomer cement (ketac cem), resin-modified glass ionomer cement (fuji ortho lc), chemically-cured composite resin (concise) and light-cured composite resin (transbond xt). s. sanguis was used as ...200616373451
[study of antibacterial effect of galla chinensis on the bacteria biofilm in chemostat].to compare the antibacterial effect of galla chinensis with that of chlorhexidine by means of chemostat and provide experimental foundation for caries prevention with chinese medicine instead of chemicals in future.200516400949
interleukin 1alpha increases the susceptibility of rabbits to experimental viridans streptococcal endocarditis.major predisposing conditions for infective endocarditis (ie) are the presence of a cardiac platelet-fibrin vegetation and of circulating bacteria with relatively low susceptibility to microbicidal activity of blood platelets. the influence of proinflammatory conditions on development of ie is unknown. we studied the effects of the presence of a catheter, inserted to induce platelet-fibrin vegetations, and of the proinflammatory cytokine interleukin-1alpha in rabbit experimental ie. leaving the ...200616428739
[infectious endocarditis complicated with preoperative cerebral infarction and rupture of infectious intracranial aneurysm].a surgically treated case of infectious endocarditis (ie) complicated with preoperative cerebral infarction and rupture of mycotic intracranial aneurysm was reported. a 66-year-old male was admitted with the diagnosis of active ie due to streptococcus sanguis, complicated with cerebral infarction 17 days previously. preoperative echocardiography showed mobile vegetations both on the aortic and the mitral leaflet, sizes of which were 12.6 and 25 mm. the magnetic resonance imaging (mri) demonstrat ...200616440688
photosensitization of in vitro biofilms by toluidine blue o combined with a light-emitting diode.in natural ecosystems, micro-organisms grow preferentially attached to surfaces, forming matrix-enclosed biofilms. the aim of this study was to determine photodestruction levels in biofilms after subjecting them to photodynamic therapy. biofilms of streptococcus mutans, s. sobrinus, and s. sanguinis were grown on enamel slabs for 3, 5 or 7 d. both the number of viable micro-organisms and the concentration of water-insoluble polysaccharides were analysed, and mineral loss (deltaz) analyses were p ...200616460343
multivariate analysis of surface physico-chemical properties controlling biofilm formation on orthodontic adhesives prior to and after fluoride and chlorhexidine treatment.biofilm formation on orthodontic adhesives is a serious clinical problem, as it leads to enamel demineralization around fixed orthodontic appliances, often leaving white spot lesions after their removal. the aim of this work was to determine the influence of surface physico-chemical properties of four commonly used orthodontic adhesives (concise, fuji ortho lc, ketac cem mu, and transbond xt) on early bacterial biofilm formation. in addition, effects of two commercially available mouthrinses (0. ...200616470828
effect of a stannous fluoride dentifrice on the sulcular microbiota: a prospective cohort study in subjects with various levels of periodontal inflammation.to assess the effects of an experimental 0.454% stannous fluoride (snf2) dentifrice on the oral sulcular microbiota in patients with various stages of oral diseases using checkerboard dna-dna hybridization.200516475455
is pre-term labour associated with periodontitis in a danish maternity ward?to reveal differences in periodontal status and presence of subgingival bacteria in a scandinavian population of women with pre-term birth compared with women who delivered at term.200616489943
bacterial endocarditis with dual presentation as endophthalmitis and glomerulonephritis.we report a case of streptococcus sanguis endocarditis in a 45-year-old male who presented with bilateral endophthalmitis and glomerulonephritis. the patient responded favorably to appropriate antimicrobial chemotherapy with resolution of endophthalmitis and nephritis. some striking features of this case and a comparison with other reported cases of this uncommon presentation of infective endocarditis are discussed.200616490690
anticariogenic activity of macelignan isolated from myristica fragrans (nutmeg) against streptococcus mutans.the occurrence of dental caries is mainly associated with oral pathogens, especially cariogenic streptococcus mutans. preliminary antibacterial screening revealed that the extract of myristica fragrans, widely cultivated for the spice and flavor of foods, possessed strong inhibitory activity against s. mutans. the anticariogenic compound was successfully isolated from the methanol extract of m. fragrans by repeated silica gel chromatography, and its structure was identified as macelignan by inst ...200616492529
a role for immunoglobulin g in donor-specific streptococcus sanguis-induced platelet aggregation.there is increasing evidence for a relationship between bacterial infections and several cardiovascular disorders. although the precise mechanism(s) underlying this association is unknown, the direct activation of platelets by bacteria is one possibility. individual strains of s. sanguis activate platelets in a non-uniform, donor-dependent manner. in the current study, platelet aggregation profiles were obtained for fourteen donors in response to four strains of s. sanguis (2017-78, 133-79, sk11 ...200616493491
interactive forces between co-aggregating and non-co-aggregating oral bacterial pairs.the temporo-spatial development of plaque is governed by adhesive interactions between different co-aggregating bacterial strains and species. physico-chemically, these interactions are due to attractive lifshitz-van der waals and acid-base forces, and occur despite electrostatic repulsion and with a critical influence of temperature. the forces between co-aggregating and non-co-aggregating pairs have never been measured, however. the aim here, thus, is to investigate, by atomic force microscopy ...200616498069
role of il-12b promoter polymorphism in adamantiades-behcet's disease susceptibility: an involvement of th1 immunoreactivity against streptococcus sanguinis antigen.adamantiades-behcet's disease (abd) is a chronic inflammatory multisystem disorder. although the precise etiology is unclear, high prevalence of human leukocyte antigen (hla)-b51 predisposition and predominantly involved t-helper type 1 cells (th1)-type proinflammatory cytokines and extrinsic streptococcal infection suggest a substantial association with an immunogenetic basis and strengthens the hypothesis that il-12, a potent inducer of th-1 immune reaction, is a putative candidate in its path ...200616514412
effect of penicillin resistance on presentation and outcome of nonenterococcal streptococcal infective endocarditis.most cases of infective endocarditis are caused by nonenterococcal streptococci. the emergence of strains resistant to penicillin is increasingly recognized worldwide. this study sought to assess the effect of penicillin resistance on presentation and outcome of streptococcal endocarditis.200616534200
vancomycin tolerance, a potential mechanism for refractory gram-positive bacteremia observational study in patients with cancer.the clinical significance of infections caused by vancomycin-tolerant (vt) gram-positive organisms in patients with cancer remains unclear.200616534785
preparation and antimicrobial activity of gelatin microparticles containing propolis against oral pathogens.gelatin microparticles containing propolis ethanolic extractive solution were prepared by spray-drying technique. particles with regular morphology, mean diameter ranging of 2.27 microm to 2.48 microm, and good entrapment efficiency for propolis were obtained. the in vitro antimicrobial activity of microparticles was evaluated against microorganisms of oral importance (enterococcus faecalis, streptococcus salivarius, streptococcus sanguinis, streptococcus mitis, streptococcus mutans, streptococc ...200616537203
Displaying items 1901 - 2000 of 2523