Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| in vivo differences in the virulence, pathogenicity, and induced protective immunity of wboa mutants from genetically different parent brucella spp. | to explore the effects of the genetic background on the characteristics of wboa gene deletion rough mutants generated from different parent brucella sp. strains, we constructed the rough-mutant strains brucella melitensis 16 m-mb6, b. abortus 2308-sb6, b. abortus s19-rb6, and b. melitensis ni-nb6 and evaluated their survival, pathogenicity, and induced protective immunity in mice and sheep. in mice, the survival times of the four mutants were very different in the virulence assay, from less than ... | 2013 | 23239800 |
| yersinia enterocolitica serotype o:9 cultured from swedish sheep showing serologically false-positive reactions for brucella melitensis. | in a herd of 20 sheep in sweden, a country where brucellosis has never been diagnosed in sheep or goats, a total of six sheep were found serologically positive to brucella melitensis in two different rounds of sampling. yersinia enterocolitica serotype o:9 could at the time of the second sampling be isolated from four sheep, one of them at the same time serologically positive for b. melitensis. the article describes the case and gives some background information on brucellosis and y. enterocolit ... | 2012 | 23240071 |
| cell mediated immune response after challenge in omp25 liposome immunized mice contributes to protection against virulent brucella abortus 544. | brucellosis is a disease affecting various domestic and wild life species, and is caused by a bacterium brucella. keeping in view the serious economic and medical consequences of brucellosis, efforts have been made to prevent the infection through the use of vaccines. cell-mediated immune responses [cmi] involving interferon gamma and cytotoxic cd4(+) and cd8(+) t cells are required for removal of intracellular brucella. omp25 has been reported to be involved in virulence of brucella melitensis, ... | 2012 | 23273966 |
| safety and efficacy of reduced doses of brucella melitensis strain rev. 1 vaccine in pregnant iranian fat-tailed ewes. | brucellosis is one of the most important zoonotic diseases and is a significant cause of abortion in animals. brucella melitensis strain rev. 1 is recommended as the most effective vaccine for small ruminants but the application of full doses in adult animals is restricted. this study was conducted to determine a proper reduced dose of vaccine which confers protection but which is not abortifacient in iranian fat-tailed sheep. a total of 51 non-vaccinated pregnant ewes were divided into three ma ... | 2012 | 23277121 |
| seroprevalence of brucellosis in livestock within three endemic regions of the country of georgia. | brucellosis is the one of most common livestock zoonoses in georgia, resulting in significant economic losses. livestock were sampled in three regions of georgia (kakheti, kvemo kartli, imereti). districts that historically reported high numbers of brucellosis related morbidity were selected for serological, bacteriological and molecular surveys. surveying efforts yielded samples from 10,819 large and small ruminants. in total, 735 serological tests were positive on rose bengal and 33 bacterial ... | 2012 | 23287714 |
| identification of the virb operon genes encoding the type iv secretion system, in colombian brucella canis isolates. | canine brucellosis is a zoonotic disease caused by brucella canis. the establishment of intracellular replicative niches of b. canis is mediated by proteins secreted by the type iv secretion system, which is encoded by the virb operon. the characterization of such genes has been conducted in other species of the genus, but not in b. canis. we report the design of a multiplex pcr test for the detection of the virb operon genes of b. canis. primers for each of the 12 genes were designed and evalua ... | 2012 | 23290573 |
| peptide nucleic acids inhibit growth of brucella suis in pure culture and in infected murine macrophages. | peptide nucleic acids (pnas) are single-stranded, synthetic nucleic acid analogues containing a pseudopeptide backbone in place of the phosphodiester sugar-phosphate. when pnas are covalently linked to cell-penetrating peptides (cpps) they readily penetrate the bacterial cell envelope, inhibit expression of targeted genes and cause growth inhibition both of gram-positive and gram-negative bacteria. however, the effectiveness of pnas against brucella, a facultative intracellular bacterial pathoge ... | 2013 | 23305655 |
| diverse genetic regulon of the virulence-associated transcriptional regulator mucr in brucella abortus 2308. | the ros-type regulator mucr is one of the few transcriptional regulators that have been linked to virulence in brucella. here, we show that a brucella abortus in-frame mucr deletion strain exhibits a pronounced growth defect during in vitro cultivation and, more importantly, that the mucr mutant is attenuated in cultured macrophages and in mice. the genetic basis for the attenuation of brucella mucr mutants has not been defined previously, but in the present study the genes regulated by mucr in ... | 2013 | 23319565 |
| a foodborne outbreak of brucellosis at a police station cafeteria, lima, peru. | brucella melitensis is highly infectious for humans and can be transmitted to humans in a number of epidemiological contexts. within the context of an ongoing brucellosis surveillance project, an outbreak at a peruvian police officer cafeteria was discovered, which led to active surveillance (serology, blood culture) for additional cases among 49 police officers who had also eaten there. the cohort was followed up to 18 months regardless of treatment or symptoms. active surveillance estimated th ... | 2013 | 23382164 |
| erythritol triggers expression of virulence traits in brucella melitensis. | erythritol is a four-carbon sugar preferentially utilized by brucella spp. the presence of erythritol in the placentas of goats, cows, and pigs has been used to explain the localization of brucella to these sites and the subsequent accumulation of large amounts of bacteria, eventually leading to abortion. here we show that brucella melitensis will also localize to an artificial site of erythritol within a mouse, providing a potential model system to study the pathogenesis of brucella abortion. i ... | 2013 | 23421980 |
| immune reactivity of brucella melitensis-vaccinated rabbit serum with recombinant omp31 and dnak proteins. | brucella melitensis infection is still a major health problem for human and cattle in developing countries and the middle east. | 2013 | 23467315 |
| molecular epidemiology and antibiotic susceptibility of livestock brucella melitensis isolates from naryn oblast, kyrgyzstan. | the incidence of human brucellosis in kyrgyzstan has been increasing in the last years and was identified as a priority disease needing most urgent control measures in the livestock population. the latest species identification of brucella isolates in kyrgyzstan was carried out in the 1960s and investigated the circulation of brucella abortus, b. melitensis, b. ovis, and b. suis. however, supporting data and documentation of that experience are lacking. therefore, typing of brucella spp. and ide ... | 2013 | 23469294 |
| importance of identification and typing of brucellae from west african cattle: a review. | bovine brucellosis is an endemic infectious disease which can impact cattle productivity and welfare negatively, as well as human health. sufficient knowledge on its epidemiology, particularly on species and biotypes of brucella at national and/or regional scale are important to set up and implement efficient control measures against brucellosis in a "one health" perspective. the main objective of this review was to investigate available literature on strains of brucella in order to provide a st ... | 2013 | 23499188 |
| factors associated with seropositive antibodies to brucella melitensis in the nakhon nayok, thailand. | human brucellosis is a re-emerging disease in thailand. in 2006, her royal highness princess maha chakri sirindhorn medical center nakorn nayok province had diagnosed three cases of brucellosis which have never been reported in this area. | 2012 | 23513464 |
| osteoarticular tissue infection and development of skeletal pathology in murine brucellosis. | brucellosis, a frequent bacterial zoonosis, can produce debilitating chronic disease with involvement of multiple organs in human patients. whereas acute brucellosis is well studied using the murine animal model, long-term complications of host-pathogen interaction remain largely elusive. human brucellosis frequently results in persistent, chronic osteoarticular system involvement, with complications such as arthritis, spondylitis and sacroiliitis. here, we focused on identifying infectious site ... | 2013 | 23519029 |
| immunogenic and invasive properties of brucella melitensis 16m outer membrane protein vaccine candidates identified via a reverse vaccinology approach. | brucella is the etiologic agent of brucellosis, one of the most common and widely distributed zoonotic diseases. its highly infectious nature, the insidious, systemic, chronic, debilitating aspects of the disease and the lack of an approved vaccine for human use in the united states are features that make brucella a viable threat to public health. one of the main impediments to vaccine development is identification of suitable antigens. in order to identify antigens that could potentially be use ... | 2013 | 23533646 |
| in vitro antimicrobial susceptibility of brucella melitensis isolates from sheep in an area endemic for human brucellosis in turkey. | the aim of this study was to assess in vitro antimicrobial susceptibility of brucella melitensis isolates isolated from naturally infected sheep cases in an area where human brucellosis is endemic, focusing on rifampin (rif), streptomycin (sm), ciprofloxacin (cpfx), trimethoprim/sulfamethoxazole (tmp/smz), gentamicin (gm) and tetracycline (tc) and on 11 other antimicrobials. the identification and typing of brucella isolates were carried out using standard classification tests and polymerase cha ... | 2013 | 23545462 |
| interactions of lipopolysaccharide with lipid membranes, raft models - a solid state nmr study. | lipopolysaccharide (lps) is a major component of the external leaflet of bacterial outer membranes, key pro-inflammatory factor and an important mediator of host-pathogen interactions. in host cells it activates the complement along with a pro-inflammatory response via a tlr4-mediated signalling cascade and shows preference for cholesterol-containing membranes. here, we use solid state (13)c and (31)p mas nmr to investigate the interactions of lps from three bacterial species, brucella melitensi ... | 2013 | 23567915 |
| congenital brucellosis in an infant. | brucellosis has been reported mainly among pregnant women, and it may lead to spontaneous abortion, intrauterine fetal death, or delivery of an infected neonate. transmission through breast milk has also been described, but congenital cases are not commonly reported. we present the clinical findings, laboratory studies, treatment, and final outcome of a late prenatal transmission from a mother to her term infant of brucella melitensis biovar 1. because the maternal disease was undetected due to ... | 2013 | 23590322 |
| evaluation of the immunogenicity and the protective efficacy in mice of a dna vaccine encoding sp41 from brucella melitensis. | brucella melitensis is a facultative intracellular gram-negative bacterial pathogen that may enter the host via ingestion or inhalation, or through conjunctiva or skin abrasions. some brucella spp surface proteins (sps) play an important role in bacterial adhesion and invasion and thus represent targets for the host immune system. brucella spp surface protein with apparent molecular mass of 41 kda interacts selectively with hela cells. | 2013 | 23592643 |
| replication of brucella melitensis inside primary human monocytes depends on mitogen activated protein kinase signaling. | the clinical course of infections caused by brucella is linked to its capacity to modulate the initial immune response of macrophages in order to ensure its intracellular replication. signal transduction pathways implicated in the survival of brucella in human cells are not completely elucidated. we herein investigated the involvement of the tlr-mapk-dependent signaling pathways in the survival of brucella in primary human monocytes using live clinical strains of brucella melitensis. b. melitens ... | 2013 | 23628412 |
| immune response and serum bactericidal activity against brucella ovis elicited using a short immunization schedule with the polymeric antigen blsomp31 in rams. | brucella ovis is the etiologic agent of ovine brucellosis. the control measures for this disease are periodical diagnosis by serological tests and/or bacteriological culture and culling of positive animals. vaccination with brucella melitensis rev 1 is recommended when prevalence is high. this attenuated strain vaccine gives protection against b. ovis but it has important disadvantages associated with the development of antibodies interfering with serodiagnosis, virulence for humans and the proh ... | 2013 | 23643287 |
| the antibacterial activity of selected labiatae (lamiaceae) essential oils against brucella melitensis. | brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. the major cause of brucellosis worldwide is brucella melitensis. medicinal plants are considered as new antibacterial sources that could replace conventional antibiotics in the treatment of antibiotic-resistant bacteria. the aim of this study was to evaluate the efficacy of some native plants, alone and in combination with some antibiotics, in the treatment of brucellosis. | 2013 | 23645957 |
| brucella melitensis 16mδhfq attenuation confers protection against wild-type challenge in balb/c mice. | brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. although effective, the current brucella vaccines (rev.1 and m5-90) have several drawbacks. the first involves residual virulence for animals and humans and the second is the inability to differentiate natural infection from that caused by vaccination. therefore, brucella melitensis 16m hfq mutant (16mδhfq) was constructed to overcome these drawbacks. similarly to rev.1 and m5-90, 16mδhfq reduces surviv ... | 2013 | 23647412 |
| hydrophobic gentamicin-loaded nanoparticles are effective against brucella melitensis infection in mice. | the clinical management of human brucellosis is still challenging and demands in vitro active antibiotics capable of targeting the pathogen-harboring intracellular compartments. a sustained release of the antibiotic at the site of infection would make it possible to reduce the number of required doses and thus the treatment-associated toxicity. in this study, a hydrophobically modified gentamicin, gentamicin-aot [aot is bis(2-ethylhexyl) sulfosuccinate sodium salt], was either microstructured or ... | 2013 | 23650167 |
| reinvestigation of the structure of brucella o-antigens. | o-specific polysaccharides of brucella contain two antigenic determinants, called a and m. most of the strains express epitope a with a small amount of epitope m, whereas brucella melitensis strain 16 m expresses longer polymer consisting mostly of m-type epitopes. proposed explanation was that epitope a is defined by 1-2-linked homopolymer of n-formylperosamine (rha4nfo), while epitope m is a pentasaccharide with four 2- and one 3-substituted rha4nfo. we reinvestigated both types of structures ... | 2013 | 23664729 |
| brucellosis in guangdong province, people's republic of china, 2005-2010. | 2013 | 23697913 | |
| a duplex pcr for rapid and simultaneous detection of brucella spp. in human blood samples. | to design a duplex pcr for rapid and simultaneous detection of brucella species. in human blood samples. | 2013 | 23711705 |
| comparison of genomes of brucella melitensis m28 and the b. melitensis m5-90 derivative vaccine strain highlights the translation elongation factor tu gene tuf2 as an attenuation-related gene. | brucella melitensis causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. attenuated b. melitensis strain m5-90, derived from virulent strain m28, is widely used as a live vaccine in ruminants in china. genetic differences between the strains may cast light on the mechanism of attenuation. we recently reported the complete genomic sequences of m28 and m5-90. genome organization is highly conserved between these isolates, and also with virulent strains 16 m and atcc 23457. ... | 2013 | 23716607 |
| brucella bior regulator defines a complex regulatory mechanism for bacterial biotin metabolism. | the enzyme cofactor biotin (vitamin h or b7) is an energetically expensive molecule whose de novo biosynthesis requires 20 atp equivalents. it seems quite likely that diverse mechanisms have evolved to tightly regulate its biosynthesis. unlike the model regulator bira, a bifunctional biotin protein ligase with the capability of repressing the biotin biosynthetic pathway, bior has been recently reported by us as an alternative machinery and a new type of gntr family transcriptional factor that ca ... | 2013 | 23729648 |
| silencing of prp c (prion protein) expression does not affect brucella melitensis infection in human derived microglia cells. | cellular prion proteins (prp(c)) are mainly expressed in the central nervous system where they have antioxidant effects and a role in the endocytosis of bacteria within cells. these proteins also have some crucial biological functions including roles in neurotransmission, signal transduction and programmed cell death. however, the role of prion proteins in neuronal brucella infection, specifically in the interaction of the pathogen and the host cell is controversial. in the present study, the si ... | 2013 | 23820446 |
| a literature review of laboratory-acquired brucellosis. | brucellosis is a bacterial zoonotic disease which has been associated with laboratory-acquired infections. no recent reviews have addressed the characteristics of laboratory-acquired brucellosis (lab). english-language literature was reviewed to identify reports of laboratory exposures to brucella spp. and lab cases between 1982 and 2007. evaluation of 28 case reports identified 167 potentially exposed laboratory workers, of whom 71 had lab. nine reports were identified that summarized an additi ... | 2013 | 23824774 |
| investigating genetic diversity of brucella abortus and brucella melitensis in italy with mlva-16. | despite the eradication of brucellosis from most of europe, the disease remains relatively common in a variety of livestock in southern european countries. it is therefore surprising that with such high prevalence rates, there have been few genetic characterizations of brucellosis outbreaks in this region. we conducted a genetic assessment of 206 isolates of brucella abortus and b. melitensis from italy using variable number tandem repeats (vntrs). we determined genetic diversity and geographic ... | 2013 | 23831636 |
| protective effect of a dna vaccine containing an open reading frame with homology to an abc-type transporter present in the genomic island 3 of brucella abortus in balb/c mice. | the immunogenicity of a dna vaccine containing an open reading frame (orf) of genomic island 3 (gi-3), specific for brucella abortus and brucella melitensis, has been examined. intramuscular injection of plasmid dna carrying the open reading frame with homology to an abc-type transporter (pv278a) into balb/c mice elicited both humoral and cellular immune responses. mice injected with pv278a had a dominant immunoglobulin g2a (igg2a) response. this dna vaccine elicited a t-cell-proliferative respo ... | 2013 | 23834811 |
| epidemiology of brucellosis in domestic animals caused by brucella melitensis, brucella suis and brucella abortus. | brucellosis is a disease that causes severe economic losses for livestock farms worldwide. brucella melitensis, b. abortus and b. suis, which are transmitted between animals both vertically and horizontally, cause abortion and infertility in their primary natural hosts - goats and sheep (b. melitensis), cows (b. abortus) and sows (b. suis). brucella spp. infect not only their preferred hosts but also other domestic and wild animal species, which in turn can act as reservoirs of the disease for o ... | 2013 | 23837364 |
| recent developments in livestock and wildlife brucellosis vaccination. | live attenuated brucellosis vaccines have been available for protecting domestic livestock against brucella melitensis and b. abortus for more than 60 years. current vaccines are effective in preventing abortion and transmission of brucellosis, but poor at preventing infection or seroconversion. in addition, they can induce abortions in pregnant animals and are infectious to humans. it can be argued that current vaccines were developed empirically in that the immunological mechanism(s) of action ... | 2013 | 23837378 |
| isolation and identification of bovine brucella isolates from pakistan by biochemical tests and pcr. | brucellosis is endemic in bovines in pakistan. the brucella species and biovars involved, however, are unknown. the objectives of the present study were to isolate and characterize brucellae from seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes which had recently aborted. the seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes were collected from the potohar plateau, pakistan. isolation of brucellae was done on modified farre ... | 2014 | 23868548 |
| a brucellosis disease control strategy for the kakheti region of the country of georgia: an agent-based model. | brucellosis has been reported in livestock and humans in the country of georgia with brucella melitensis as the most common species causing disease. georgia lacked sufficient data to assess effectiveness of the various potential control measures utilizing a reliable population-based simulation model of animal-to-human transmission of this infection. therefore, an agent-based model was built using data from previous studies to evaluate the effect of an animal-level infection control programme on ... | 2014 | 23879523 |
| rapid quantitative detection of brucella melitensis by a label-free impedance immunosensor based on a gold nanoparticle-modified screen-printed carbon electrode. | a rapid and simple method for quantitative monitoring of brucella melitensis using electrochemical impedance spectroscopy (eis) is reported for the first time. the label-free immunosensors were fabricated by immobilizing brucella melitensis antibody on the surface of gold nanoparticle-modified screen-printed carbon electrodes (gnp-spces). cyclic voltammetry (cv) and eis were used to characterize the brucella melitensis antigen interaction on the surface of gnp-spces with antibody. a general elec ... | 2013 | 23881126 |
| the effects of mucr on expression of type iv secretion system, quorum sensing system and stress responses in brucella melitensis. | mucr is a transcriptional regulator in many bacterial pathogens and is required for virulence in mice and macrophages, resistance to stress responses, and modification of the cell envelope in brucella spp. to determine why the mucr deleted mutant is attenuated in vivo and in vitro, we performed rna-seq analysis using brucella melitensis rna obtained from b. melitensis 16m and 16mδmucr grown under the same conditions. we found 442 differentially expressed genes; 310 were over expressed, and 132 w ... | 2013 | 23932078 |
| pacemaker infection due to brucella melitensis. | 2012 | 23935331 | |
| serologic detection of antibodies to brucella spp. using a commercial elisa in cattle in grenada, west indies. | bovine brucellosis, caused mainly by brucella abortus, a zoonotic bacterium, has been reported from many areas of the world, including central and south america, and the caribbean island state of trinidad and tobago. although brucellosis has been eradicated from domestic cattle in canada it still exists in one or two herds in the united states. serological tests are important in estimating prevalence of brucella exposure in order to target eradication programmes. in this study, serum samples fro ... | 2013 | 23959493 |
| comparative genomic analysis of brucella melitensis vaccine strain m5 provides insights into virulence attenuation. | the brucella melitensis vaccine strain m5 is widely used to prevent and control brucellosis in animals. in this study, we determined the whole-genome sequence of m5, and conducted a comprehensive comparative analysis against the whole-genome sequence of the virulent strain 16 m and other reference strains. this analysis revealed 11 regions of deletion (rds) and 2 regions of insertion (ris) within the m5 genome. among these regions, the sequences encompassed in 5 rds and 1 ri showed consistent va ... | 2013 | 23967122 |
| impact of hfq on global gene expression and intracellular survival in brucella melitensis. | brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. the ability of brucellae to survive and multiply in the hostile environment of host macrophages is essential to its virulence. the rna-binding protein hfq is a global regulator that is involved in stress resistance and pathogenicity. here we demonstrate that hfq is essential for stress adaptation and intracellular survival in b. melitensis. a b. melitensis hfq deletion mutant exhibits reduced surviva ... | 2013 | 23977181 |
| brucella melitensis infection of ventriculo-peritoneal shunt: a form of neurobrucellosis manifested as gastrointestinal symptoms. | a report of a 9 year-old child with myelomeningocele who has a ventriculo-peritoneal (vp) shunt presented with gastrointestinal symptoms and peritonitis. the patient had no cns symptoms but the cerebrospinal fluid was positive for brucella melitensis. we discuss neurobrucellosis in children, its various presentations, complications and challenges in treatment, choice of antibiotics and duration. | 2014 | 23999355 |
| in vitro synergistic activity of antibiotic combinations against brucella melitensis using e-test methodology. | the treatment of brucellosis is still problematic, because of high rates of treatment failure or relapses. as the microorganism is an intracellular pathogen, treatment requires combined regimens. however, limited existing data on in vitro combinations are avaliable for brucellae. the aim of this study was to investigate the in vitro efficacy of various traditional and new antibiotic combinations against 16 brucella melitensis strains. the combination effect of antimicrobial agents was evaluated ... | 2008 | 24031207 |
| bacteriological features of infectious spondylodiscitis at mohammed v military teaching hospital of rabat. | to review the bacteriological features of infectious spondylodiscitis and provide recommendations for the initial therapy which remains empirical in our context. retrospective study including patients diagnosed with spondylodiscitis over a period of 4 years (2006-2009) at the rabat military teaching hospital. during the study period, we analysed 30 cases: the mean age was 49.9 years and 21 cases (70%) were male. the patients were predominantly hospitalized in neurosurgery department (15/30) foll ... | 2012 | 24031960 |
| brucellosis in a renal transplant recipient. | brucellosis is one of the most common systemic zoonotic diseases transmitted by consumption of unpasteurized dairy products or by occupational contact with infected animals. brucellosis is rare in renal transplant recipients. only 3 cases have been reported in the literature. we report a case of brucellosis with hematologic and hepatobiliary complications in a patient 3 years after renal transplantation. the mean time from transplantation to the diagnosis of brucellosis in these 4 reported patie ... | 2013 | 24034171 |
| ex vivo innate immune cytokine signature of enhanced risk of relapsing brucellosis. | brucellosis, a zoonotic infection caused by one of the gram-negative intracellular bacteria of the brucella genus, is an ongoing public health problem in perú. while most patients who receive standard antibiotic treatment recover, 5-40% suffer a brucellosis relapse. in this study, we examined the ex vivo immune cytokine profiles of recovered patients with a history of acute and relapsing brucellosis. | 2013 | 24040434 |
| laboratory exposure to brucella melitensis in denmark: a prospective study. | brucella species are a frequent cause of laboratory-acquired infections. this report describes the handling of a laboratory exposure of 17 laboratory staff members exposed to brucella melitensis in a large microbiology laboratory in a brucella-non-endemic area. we followed the us centers for disease control and prevention guidelines, but, of 14 staff members classified as high-risk exposure, none accepted post-exposure prophylaxis. however, in a period of 6 months of follow-up, none of the expos ... | 2013 | 24070633 |
| in vitro drug resistance of clinical isolated brucella against antimicrobial agents. | to explore the antibiotic resistance of brucella melitensis and instruct rational use of antimicrobial agents in clinical treatment of brucella infection. | 2013 | 24083592 |
| cloning, expression, purification, crystallization and preliminary x-ray crystallographic analysis of the tir domain from the brucella melitensis tir-domain-containing protein tcpb. | in mammals, toll-like receptors (tlrs) recognize conserved microbial molecular signatures and induce an early innate immune response in the host. tlr signalling is mediated by interactions between the cytosolic tir (toll/interleukin-1 receptor) domains of the receptor and the adaptor proteins. increasingly, it is apparent that pathogens target this interaction via pathogen-expressed tir-domain-containing proteins to modulate immune responses. a tir-domain-containing protein tcpb has been reporte ... | 2013 | 24100574 |
| draft genome sequence of brucella melitensis strain admas-g1, isolated from placental fluids of an aborted goat. | here, we report the draft genome sequence and annotation of the brucella melitensis strain designated admas-g1, isolated from placental fluids of an aborted goat. the length of the genome is 3,284,982 bp, with a 57.3% gc content. a total of 3,325 protein-coding genes and 63 rna genes were predicted. | 2013 | 24115541 |
| mlva genotyping of brucella melitensis and brucella abortus isolates from different animal species and humans and identification of brucella suis vaccine strain s2 from cattle in china. | in china, brucellosis is an endemic disease and the main sources of brucellosis in animals and humans are infected sheep, cattle and swine. brucella melitensis (biovars 1 and 3) is the predominant species, associated with sporadic cases and outbreak in humans. isolates of b. abortus, primarily biovars 1 and 3, and b. suis biovars 1 and 3 are also associated with sporadic human brucellosis. in this study, the genetic profiles of b. melitensis and b. abortus isolates from humans and animals were a ... | 2013 | 24124546 |
| brucella melitensis t cell epitope recognition in humans with brucellosis in peru. | brucella melitensis, one of the causative agents of human brucellosis, causes acute, chronic, and relapsing infection. while t cell immunity in brucellosis has been extensively studied in mice, no recognized human t cell epitopes that might provide new approaches to classifying and prognosticating b. melitensis infection have ever been delineated. twenty-seven pools of 500 major histocompatibility complex class ii (mhc-ii) restricted peptides were created by computational prediction of promiscuo ... | 2014 | 24126518 |
| hepatic brucelloma. | we present a case of hepatic abscess caused by brucella melitensis (or hepatic brucelloma) diagnosed in a 59-year-old man 33 years after an episode of acute brucellosis that had completely resolved. recovery from symptoms and a decrease in lesion size seen on radiological assessment were achieved through prolonged combined antibiotic therapy, without the need for surgery. hepatic brucelloma is a rare complication of brucellosis, which is the most common zoonosis globally, mainly occurring in spe ... | 2013 | 24156899 |
| effect of medium ph on antibiotic activity against syrian brucella spp. isolates. | brucellosis is an endemic zoonosis in syria, affecting large numbers of animals. there are an increasing number of cases in humans. brucella is a facultative intracellular pathogen, a small, non-motile, gram-negative coccobacillus, which causes abortion in domestic animals and a febrile illness in humans. | 2013 | 24174696 |
| deletion of the gi-2 integrase and the wbka flanking transposase improves the stability of brucella melitensis rev 1 vaccine. | brucella melitensis rev 1 is the best vaccine available for the prophylaxis of small ruminant brucellosis and, indirectly, for reducing human brucellosis. however, rev 1 shows anomalously high rates of spontaneous dissociation from smooth (s) to rough (r) bacteria, the latter being inefficacious as vaccines. this s-r instability results from the loss of the o-polysaccharide. to overcome this problem, we investigated whether some recently described mechanisms promoting mutations in o-polysacchari ... | 2013 | 24176078 |
| an unusual case of neurobrucellosis presenting as demyelination disorder. | brucellosis is a public health problem in most countries in the mediterranean. involvement of the central nervous system is seen in 4-13% of patients with brucellosis. a 13-year-old girl was admitted because of gait disturbance, diplopia, and dizziness. her complaints began about 1.5 years ago. the second symptomatic episode repeated about three months ago and the third two months ago. in total, attacks repeated 3 times over 1.5 years. the magnetic resonance imaging (mri) and the clinical featur ... | 2013 | 24192684 |
| brucellosis as an aetiology of septic arthritis. | brucellosis is a zoonotic disease caused by a gram-negative coccobacillus from the brucella genus. the disease has a broad spectrum of clinical manifestations. the musculoskeletal system involvement is frequent and, rarely, arthritis can be the only clinical feature of the disease. we report a case of monoarthritis caused by brucella melitensis. | 2014 | 24231687 |
| mechanism of bacterial interference with tlr4 signaling by brucella toll/interleukin-1 receptor domain-containing protein tcpb. | upon activation of toll-like receptors (tlrs), cytoplasmic toll/interleukin-1 receptor (tir) domains of the receptors undergo homo- or heterodimerization. this in turn leads to the recruitment of adaptor proteins, activation of transcription factors, and the secretion of pro-inflammatory cytokines. recent studies have described the tir domain-containing protein from brucella melitensis, tcpb (btpa/btp1), to be involved in virulence and suppression of host innate immune responses. tcpb interferes ... | 2013 | 24265315 |
| cd8 knockout mice are protected from challenge by vaccination with wr201, a live attenuated mutant of brucella melitensis. | cd8+ t cells have been reported to play an important role in defense against b. abortus infection in mouse models. in the present report, we use cd8 knockout mice to further elucidate the role of these cells in protection from b. melitensis infection. mice were immunized orally by administration of b. melitensis wr201, a purine auxotrophic attenuated vaccine strain, then challenged intranasally with b. melitensis 16m. in some experiments, persistence of wr201 in the spleens of cd8 knockout mice ... | 2013 | 24288554 |
| prematurity due to maternal brucella infection and review of the literature. | brucellosis is a zoonosis caused by ingestion of unpasteurized milk or other dairy products from infected animals or through close contact with their secretions. human-to-human transmission, which is rare, has been reported in association with blood transfusion, bone marrow transplantation, transplacental or perinatal exposure, and breastfeeding. in the neonatal period, congenital brucellosis, which is an extremely rare condition, can lead to serious clinical consequences with a high morbidity a ... | 2013 | 24292039 |
| skeletal involvement of brucella melitensis in children: a systematic review. | brucellosis is a protean disease and should be excluded in any febrile child with a constellation of symptoms such as fever, malaise, sweating, arthralgia, and joint swelling in endemic areas. skeletal system involvement is the most common source of complaints in brucellosis. the frequency of skeletal involvement in children is 6.4% to 73.5%. there are some controversies regarding the most common sites of involvement: sacroiliac versus peripheral joints. in the vast majority of cases, peripheral ... | 2013 | 24293781 |
| spinal brucellosis in south of tunisia: review of 32 cases. | brucellosis remains an important economic and public health problem in some parts of the world. the spine is the most common site of musculoskeletal involvement of brucellosis. | 2014 | 24331843 |
| cross-institute evaluations of inhibitor-resistant pcr reagents for direct testing of aerosol and blood samples containing biological warfare agent dna. | rapid pathogen detection is crucial for the timely introduction of therapeutics. two groups (one in the united kingdom and one in the united states) independently evaluated inhibitor-resistant pcr reagents for the direct testing of substrates. in the united kingdom, a multiplexed bacillus anthracis (target) and bacillus subtilis (internal-control) pcr was used to evaluate 4 reagents against 5 pcr inhibitors and down-selected the taqman fast virus 1-step master mix (life technologies inc.). in th ... | 2014 | 24334660 |
| brucella induces an unfolded protein response via tcpb that supports intracellular replication in macrophages. | brucella melitensis is a facultative intracellular bacterium that causes brucellosis, the most prevalent zoonosis worldwide. the brucella intracellular replicative niche in macrophages and dendritic cells thwarts immune surveillance and complicates both therapy and vaccine development. currently, host-pathogen interactions supporting brucella replication are poorly understood. brucella fuses with the endoplasmic reticulum (er) to replicate, resulting in dramatic restructuring of the er. this er ... | 2013 | 24339776 |
| systems biology analysis of brucella infected peyer's patch reveals rapid invasion with modest transient perturbations of the host transcriptome. | brucella melitensis causes the most severe and acute symptoms of all brucella species in human beings and infects hosts primarily through the oral route. the epithelium covering domed villi of jejunal-ileal peyer's patches is an important site of entry for several pathogens, including brucella. here, we use the calf ligated ileal loop model to study temporal in vivo brucella-infected host molecular and morphological responses. our results document brucella bacteremia occurring within 30 min afte ... | 2013 | 24349118 |
| quorum sensing and self-quorum quenching in the intracellular pathogen brucellamelitensis. | brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such as the virb type iv secretion system and the flagellar genes. however, the basis of quorum sensing, namely the production of autoinducers in brucella has been questioned. here, we report data obtained from the use of a genetic tool allowing the in situ detection of long-chain n-acyl-homoserine lactones (ahl) activity at single bacterium level in brucella melitensis. these ... | 2013 | 24349302 |
| aerosol-induced brucellosis increases tlr-2 expression and increased complexity in the microanatomy of astroglia in rhesus macaques. | brucella melitensis, a bacterial pathogen and agent of epizootic abortion causes multiple pathologies in humans as well as a number of agriculturally important animal species. clinical human brucellosis manifests as a non-specific, chronic debilitating disease characterized by undulant fever, arthropathies, cardiomyopathies and neurological sequelae. these symptoms can occur acutely for a few weeks or persist for months to years. within the brain, endothelial and glial cells can be infected lead ... | 2013 | 24350061 |
| evaluation of humoral and cellular immune responses to bp26 and omp31 epitopes in the attenuated brucella melitensis vaccinated sheep. | in recent years, the number of cases of human brucellosis has been increasing by approximately 10% per year in china. most cases were caused by brucella melitensis through contacts with infected sheep, goats or their products. an attenuated b. melitensis vaccine m5-90 is currently used to vaccinate both animals in china. this vaccine has not been investigated for critical parameters such as immune response and its association with protective efficacy. in this study, humoral and cellular immune r ... | 2014 | 24370708 |
| increasing threat of brucellosis to low-risk persons in urban settings, china. | cases of brucellosis were diagnosed in 3-month-old twins and their mother. an epidemiologic survey suggested that raw sheep or goat meat might be the source of brucella melitensis infection. this finding implies that the increasing threat of brucellosis might affect low-risk persons in urban settings in china. | 2014 | 24377827 |
| immunotherapeutics to prevent the replication of brucella in a treatment failure mouse model. | outer membrane vesicles (omvs) from brucella melitensis and irradiated brucella neotomae have been shown to be effective vaccines against a b. melitensis challenge in a mouse model. the present study evaluates the efficacy of these two vaccines as immuno-therapeutics in combination with conventional antibiotics against a b. melitensis infection. balb/c mice chronically infected with b. melitensis were treated for 4 weeks with doxycycline and gentamicin and vaccinated twice during the course of t ... | 2014 | 24397905 |
| reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents. | the need for a rapid detection and characterization of biowarfare (bw) agents cannot be over emphasized. with diverse array of potential bw pathogen available presently, rapid identification of the pathogen is crucial, so that specific therapy and control measures can be initiated. we have developed a multiplex polymerase chain reaction based reverse line blot macroarray to simultaneously detect four pathogens of bw importance viz. bacillus anthracis, yersinia pestis, brucella melitensis and bur ... | 2013 | 24426077 |
| splenic abscess due to brucellosis: a case report and a review of the literature. | splenic abscess due to acute brucellosis is a rare event. we report a case of multiple splenic abscesses caused by brucella melitensis in a 45-year-old woman and review the english language literature based on a pubmed/medline search of the last 50 years. the majority of the cases published in the literature were due to b. melitensis and a splenectomy was required in half of the cases. antibiotics alone without surgical intervention can be successful in the treatment of patients with splenic bru ... | 2014 | 24433982 |
| antimicrobial susceptibility data for brucella melitensis isolates cultured from uk patients. | 2014 | 24440739 | |
| brucella melitensis shunt infection. | 2014 | 24441347 | |
| 16s rrna and omp31 gene based molecular characterization of field strains of b. melitensis from aborted foetus of goats in india. | brucellosis is a reemerging infectious zoonotic disease of worldwide importance. in human, it is mainly caused by brucella melitensis, a natural pathogen for goats. in india, a large number of goats are reared in semi-intensive to intensive system within the close vicinity of human being. at present, there is no vaccination and control strategy for caprine brucellosis in the country. thus, to formulate an effective control strategy, the status of etiological agent is essential. to cope up with t ... | 2013 | 24453799 |
| evaluation of different enzyme-linked immunosorbent assays for the diagnosis of brucellosis due to brucella melitensis in sheep. | six serological assays for the diagnosis of ovine brucellosis, due to brucella melitensis were evaluated. reference serum samples from sheep of known b. melitensis infection status (n=118) were assessed using the rose bengal test (rbt), complement fixation test (cft) and four commercial enzyme-linked immunosorbent assays (elisas), including two indirect elisas (ielisas), one competitive elisa (celisa) and one blocking elisa (belisa). the highest differential positive rates (dpr) were obtained wi ... | 2014 | 24456797 |
| brucella endocarditis of pseudoaneurysm of an aortic composite graft. | the occurrence of brucella endocarditis following bentall operation is a rare and life threatening condition, particularly when it is complicated by development of a pseudoaneurysm. here we present a 40-year-old veterinarian with bicuspid aortic valve, who developed type a aortic root dissection and required bentall operation. his past medical history was positive for an episode of treated brucellosis. four months after the operation, he developed signs, symptoms and serological findings of bruc ... | 2013 | 24459566 |
| a pregnant mouse model for the vertical transmission of brucella melitensis. | abortion is the major clinical sign of brucellosis in animals but little is known about the underlying mechanisms. this study was designed to evaluate a pregnant mouse model for the vertical transmission of brucella melitensis using four infectious doses: 10(3) colony-forming units (cfu), 10(4) cfu, 10(5) cfu, and 10(6) cfu. during the experimental period, no instances of abortion were recorded, but stillbirths were observed in the groups infected with doses of 10(4) cfu and higher. regardless o ... | 2014 | 24462801 |
| genetic polymorphism characteristics of brucella canis isolated in china. | in china, brucellosis is an endemic disease typically caused by brucella melitensis infection (biovars 1 and 3). brucella canis infection in dogs has not traditionally recognized as a major problem. in recent years however, brucellosis resulting from brucella canis infection has also been reported, suggesting that infections from this species may be increasing. data concerning the epidemiology of brucellosis resulting from brucella canis infection is limited. therefore, the purpose of this study ... | 2014 | 24465442 |
| brucella endocarditis in prosthetic valves. | none declared. | 2012 | 24493988 |
| evaluation of the immunogenicity and safety of brucella melitensis b115 vaccination in pregnant sheep. | in spite of its limitations, rev.1 is currently recognized as the most suitable vaccine against brucella melitensis (the causative agent of ovine and caprine brucellosis). however, its use is limited to young animals when test-and-slaughter programs are in place because of the occurrence of false positive-reactions due to rev.1 vaccination. the b. melitensis b115 rough strain has demonstrated its efficacy against b. melitensis virulent strains in the mouse model, but there is a lack of informati ... | 2014 | 24508034 |
| assessment of genetic diversity of zoonotic brucella spp. recovered from livestock in egypt using multiple locus vntr analysis. | brucellosis is endemic in most parts of egypt, where it is caused mainly by brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. knowledge of the predominant brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. for this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 brucella spp. isolates recovered from domestic ruminants (catt ... | 2014 | 24511531 |
| validation of a simple universal ielisa for the diagnosis of human brucellosis. | the definitive diagnosis of brucellosis requires isolation of the agent, although negative isolation does not rule out the infection. in contrast, serological testing is more sensitive and, therefore, preferred in clinical practice. the majority of reported cases around the world were caused by brucella melitensis, b. abortus, b. suis and b. canis. the first three species contain o-polysaccharide (ops) on the cell surface, but b. canis contains no measurable ops on the rough lipopolysaccharide ( ... | 2014 | 24515098 |
| comparative whole genome analysis of six diagnostic brucellaphages. | whole genome sequencing of six diagnostic brucellaphages, tbilisi (tb), firenze (fz), weybridge (wb), s708, berkeley (bk) and r/c, was followed with genomic comparisons including recently described genomes of the tb phage from mexico (tbm) and pr phage to elucidate genomic diversity and candidate host range determinants. comparative whole genome analysis revealed high sequence homogeneity among these brucellaphage genomes and resolved three genetic groups consistent with defined host range pheno ... | 2014 | 24530704 |
| preliminary report on the therapeutic action of methymycin in brucella melitensis infection. | 1954 | 24542881 | |
| collapsed l4 vertebral body caused by brucellosis. | brucellosis is caused by gram-negative, aerobic, non-motile, facultative, intracellular coccobacilli belonging to the genus brucella. a 50-year-old man working as an employee was admitted to neurosurgery clinic with severe low back, radicular right leg pain and hypoesthesia in right l4-5 dermatomes for 2 months. brucella tube agglutination (wright) test was positive in serum sample of the patient with a titer of 1/640. brucella melitensis was isolated from blood culture. x-ray and mri of the lom ... | 2014 | 24570819 |
| seroprevalence for coxiella burnetii, francisella tularensis, brucella abortus and brucella melitensis in austrian adults: a cross-sectional survey among military personnel and civilians. | the prevalence of coxiella burnetii, francisella tularensis, brucella abortus, and brucella melitensis infections in austria and the exposure risk of military personnel were assessed in an exploratory nationwide cross-sectional seroprevalence survey in 526 healthy adult individuals, 222 of which were soldiers and 304 were civilians. screening for iga/igg antibodies to c. burnetii (phase i) and igg/igm antibodies to c. burnetii (phase ii), and to f. tularensis was done with commercial enzyme-link ... | 2014 | 24572607 |
| [two spondylodiscitis due to a contamination by brucella melitensis in a mother and daughter back from holidays]. | we describe here a case of spondylodiscitis of brucellosis origin in one patient back from turkey. some weeks later, her mother, who had accompagnied her in turkey also developped similar symptoms. a diagnosis of spondylodiscitis due to a contamination by brucella melitensis was also proposed. since the control of animal brucellosis in europe, human cases are rarer. sporadic cases still observed are mostly travellers back from aerea where animal brucellosis remains endemic. seroprevalence of a s ... | 2014 | 24624738 |
| case-control study on risk factors associated with brucella melitensis in goat farms in peninsular malaysia. | caprine brucellosis is a bacterial zoonotic infection affecting goats especially in developing countries all over the world. in malaysia, the risk factors associated with this infection in farms have not been studied. a case-control study was carried out in goat farms in four states of malaysia to elucidate the risk factors associated with the infection on the farms using structured questionnaires and face-to-face interviews. results indicate that the introduction of new animals (or = 5.25; 90 % ... | 2014 | 24633657 |
| proteomic analysis of detergent resistant membrane domains during early interaction of macrophages with rough and smooth brucella melitensis. | the plasma membrane contains discrete nanometer-sized domains that are resistant to non-ionic detergents, and which are called detergent resistant membrane domains (drmds) or lipid rafts. exposure of host cells to pathogenic bacteria has been shown to induce the re-distribution of specific host proteins between drmds and detergent soluble membranes, which leads to the initiation of cell signaling that enable pathogens to access host cells. drmds have been shown to play a role in the invasion of ... | 2014 | 24643124 |
| humoral immunity and cd4+ th1 cells are both necessary for a fully protective immune response upon secondary infection with brucella melitensis. | brucella spp are intracellular bacteria that cause brucellosis, one of the most common zoonoses in the world. given the serious medical consequences of this disease, a safe and effective human vaccine is urgently needed. efforts to develop this vaccine have been hampered by our lack of understanding of what constitutes a protective memory response against brucella. in this study, we characterize the cells and signaling pathways implicated in the generation of a protective immune memory response ... | 2014 | 24646742 |
| crystal structure and biochemical studies of brucella melitensis 5'-methylthioadenosine/s-adenosylhomocysteine nucleosidase. | the prokaryotic 5'-methylthioadenosine/s-adenosylhomocysteine nucleosidase (mtan) catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (mta) and s-adenosylhomocysteine (sah), a process that plays a key role in several metabolic pathways. its absence in all mammalian species has implicated this enzyme as a promising target for antimicrobial drug design. here, we report the crystal structure of bmmtan in complex with its product adenine at a resolution of 2.6 å dete ... | 2014 | 24657441 |
| cold shock protein a plays an important role in the stress adaptation and virulence of brucella melitensis. | brucella melitensis is a facultative intracellular pathogen that mainly resides within macrophages. the mechanisms employed by brucella to adapt to harsh intracellular environments and survive within host macrophages are not clearly understood. here, we constructed a cspa gene deletion mutant, niδcspa, that did not exhibit any discernible growth defect at a normal culture temperature (37 °c) or at a low temperature (15 °c). however, expression of the cspa gene in brucella was induced by cold, ac ... | 2014 | 24661136 |
| the inva gene of brucella melitensis is involved in intracellular invasion and is required to establish infection in a mouse model. | some of the mechanisms underlying the invasion and intracellular survival of b. melitensis are still unknown, including the role of a subfamily of nudix enzymes, which have been described in other bacterial species as invasins and are present in brucella spp. we have generated a mutation in the coding gene of one of these proteins, the inva gene (bmei0215) of b. melitensis strain 133, to understand its role in virulence. hela cell invasion results showed that mutant strain survival was decreased ... | 2014 | 24667775 |
| brucella melitensis in france: persistence in wildlife and probable spillover from alpine ibex to domestic animals. | bovine brucellosis is a major zoonosis, mainly caused by brucella abortus, more rarely by brucella melitensis. france has been bovine brucellosis officially-free since 2005 with no cases reported in domestic/wild ruminants since 2003. in 2012, bovine and autochthonous human cases due to b. melitensis biovar 3 (bmel3) occurred in the french alps. epidemiological investigations implemented in wild and domestic ruminants evidenced a high seroprevalence (>45%) in alpine ibex (capra ibex); no cases w ... | 2014 | 24732322 |
| three-dimensional (3d) structure prediction of the american and african oil-palms β-ketoacyl-[acp] synthase-ii protein by comparative modelling. | the fatty-acid profile of the vegetable oils determines its properties and nutritional value. palm-oil obtained from the african oil-palm [elaeis guineensis jacq. (tenera)] contains 44% palmitic acid (c16:0), but, palm-oil obtained from the american oilpalm [elaeis oleifera] contains only 25% c16:0. in part, the b-ketoacyl-[acp] synthase ii (kasii) [ec: 2.3.1.179] protein is responsible for the high level of c16:0 in palm-oil derived from the african oil-palm. to understand more about e. guineen ... | 2014 | 24748752 |
| modelling risk aversion to support decision-making for controlling zoonotic livestock diseases. | zoonotic infectious livestock diseases are becoming a significant burden for both animal and human health and are rapidly gaining the attention of decision-makers who manage public health programmes. if control decisions have only monetary components, governments are generally regarded as being risk-neutral and the intervention strategy with the highest expected benefit (lowest expected net costs) should be preferred. however, preferences will differ and alternative intervention plans will preva ... | 2013 | 24761718 |