Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effective induction of acquired resistance to listeria monocytogenes by immunizing mice with in vivo-infected dendritic cells. | splenic dendritic cells (dcs) obtained from mice at 48 h after listeria monocytogenes infection exhibited up-regulation of cd80 and produced higher titers of gamma interferon (ifn-gamma) and interleukin-12 (il-12) than did dcs obtained from uninfected mice. mice immunized with dcs obtained from mice that had been infected with l. monocytogenes 48 h before acquired host resistance to lethal infection with l. monocytogenes at 4 and 8 weeks. immunization with dcs from heat-killed l. monocytogenes f ... | 2003 | 12496156 |
| identification of substrates and chaperone from the yersinia enterocolitica 1b ysa type iii secretion system. | all pathogenic yersinia enterocolitica strains carry the pyv plasmid encoding the ysc-yop type iii secretion (tts) system, which operates at 37 degrees c. in addition, biovar 1b y. enterocolitica strains possess a second, chromosomally encoded, tts system called ysa, which operates, at least in vitro, under low-temperature and high-salt (lths) conditions. six open reading frames, sycb, yspb, yspc, yspd, yspa, and acpy, neighbor the ysa genes encoding the ysa tts apparatus. here we show that yspa ... | 2003 | 12496172 |
| impact of vector priming on the immunogenicity of recombinant salmonella vaccines. | there are conflicting reports concerning the impact of prior vector priming on the immunogenicity of recombinant-salmonella-based vaccines. a comparison of experimental protocols identified two variables which might account for this inconsistency: the potential of the vector strain to colonize the murine gut-associated lymphoid tissue (galt) and the nature of the foreign antigen subsequently delivered by the recombinant salmonella construct. the former was investigated by constructing an aroa mu ... | 2003 | 12496178 |
| the salmonella enterica serovar typhimurium translocated effectors ssej and sifb are targeted to the salmonella-containing vacuole. | the salmonella enterica serovar typhimurium type iii secretion system (ttss) encoded in salmonella pathogenicity island 2 (spi-2) promotes replication within host cells and systemic infection of mice. the spi-2 ttss is expressed following salmonella internalization into host cells and translocates effectors across the membrane of the salmonella-containing vacuole (scv). two effectors with similar amino-terminal domains, ssej and sifb, localize to the scv membrane in infected hep-2 cells and subs ... | 2003 | 12496192 |
| cholera toxin induces migration of dendritic cells from the subepithelial dome region to t- and b-cell areas of peyer's patches. | intestinal m cells deliver macromolecules, particles, and pathogens into the subepithelial dome (sed) region of peyer's patch mucosa, an area rich in dendritic cells (dcs). we tested whether uptake of the mucosal adjuvant cholera toxin (ct) or live salmonella bacteria can induce dc migration within peyer's patches. virus-sized, fluorescent polystyrene microparticles were efficiently transported by m cells and ingested by cd11c(+), cd11b(-), and cd8a(-) dcs in the sed region. dcs loaded with micr ... | 2003 | 12496201 |
| oxyr acts as a repressor of catalase expression in neisseria gonorrhoeae. | it has been reported that neisseria gonorrhoeae possesses a very high level of catalase activity, but the regulation of catalase expression has not been investigated extensively. in escherichia coli and salmonella enterica serovar typhimurium, it has been demonstrated that oxyr is a positive regulator of hydrogen peroxide-inducible genes, including the gene encoding catalase. the oxyr gene from n. gonorrhoeae was cloned and used to complement an e. coli oxyr mutant, confirming its identity and f ... | 2003 | 12496210 |
| [community-acquired bacterial infections]. | corticosteroids in bacterial meningitis: a randomised multicentre study on meningitis in adults versus a placebo showed significant benefits of corticoids in terms of reduction in mortality and neurological complications. these results were essentially observed in pneumococcal infections. the interest in monitoring blood cultures: several works on staphylococcus aureus bacteremia have confirmed the necessity of monitoring hemocultures, 48-72 hours following initiation of treatment. the persisten ... | 2002 | 12497721 |
| mg2+ sensing by the mg2+ sensor phoq of salmonella enterica. | the phop/phoq two-component regulatory system governs the adaptation to low mg(2+) environments and virulence in several gram-negative species. during growth in low mg(2+), the sensor phoq modifies the activity of the response regulator phop promoting gene transcription, whereas growth in high mg(2+) represses transcription of phop-activated genes. the phoq protein harbors a periplasmic domain of 146 amino acid residues that binds mg(2+) in vitro and is required for mg(2+)-mediated repression in ... | 2003 | 12507481 |
| effect of previous antimicrobial treatment on fecal shedding of salmonella enterica subsp. enterica serogroup b in new york dairy herds with recent clinical salmonellosis. | the association of herd- and sample-level factors with the isolation of salmonella group b from cattle fecal samples was analyzed. study farms were 65 dairy herds with a recent history of laboratory-confirmed clinical salmonella infections. herds were visited once per month for three months to collect data and samples for bacteriological culture. herd size varied widely from 34 to 3700 total cattle on the farm (median=370). salmonella serogroup b was isolated from 270 of 2726 samples tested. the ... | 2003 | 12507855 |
| salmonella enterica serovar typhimurium rdoa is growth phase regulated and involved in relaying cpx-induced signals. | the disulfide oxidoreductase, dsba, mediates disulfide bond formation in proteins as they enter or pass through the periplasm of gram-negative bacteria. although dsba function has been well characterized, less is known about the factors that control its expression. previous studies with escherichia coli demonstrated that dsba is part of a two-gene operon that includes an uncharacterized, upstream gene, yihe, that is positively regulated via the cpx stress response pathway. to clarify the role of ... | 2003 | 12511488 |
| transcription of the salmonella invasion gene activator, hila, requires hild activation in the absence of negative regulators. | salmonella enterica serovar typhimurium causes human gastroenteritis and a systemic typhoid-like infection in mice. infection is initiated by entry of the bacteria into intestinal epithelial cells and is mediated by a type iii secretion system that is encoded by genes in salmonella pathogenicity island 1. the expression of invasion genes is tightly regulated by environmental conditions such as oxygen and osmolarity, as well as by many bacterial factors. the hila gene encodes an ompr/toxr family ... | 2003 | 12511499 |
| genomic comparison of salmonella enterica serovars and salmonella bongori by use of an s. enterica serovar typhimurium dna microarray. | the genus salmonella consists of over 2,200 serovars that differ in their host range and ability to cause disease despite their close genetic relatedness. the genetic factors that influence each serovar's level of host adaptation, how they evolved or were acquired, their influence on the evolution of each serovar, and the phylogenic relationships between the serovars are of great interest as they provide insight into the mechanisms behind these differences in host range and disease progression. ... | 2003 | 12511502 |
| flagella and motility in actinobacillus pleuropneumoniae. | actinobacillus pleuropneumoniae has been considered nonmotile and nonflagellate. in this work, it is demonstrated that a. pleuropneumoniae produces flagella composed of a 65-kda protein with an n-terminal amino acid sequence that shows 100% identity with those of escherichia coli, salmonella, and shigella flagellins. the dna sequence obtained through pcr of the flic gene in a. pleuropneumoniae showed considerable identity (93%) in its 5' and 3' ends with the dna sequences of corresponding genes ... | 2003 | 12511514 |
| high ph during trisodium phosphate treatment causes membrane damage and destruction of salmonella enterica serovar enteritidis. | trisodium phosphate (tsp) is now widely used during the processing of poultry and red meats, but the mechanism whereby it inactivates gram-negative bacteria such salmonella spp. remains unclear. thus, salmonella enterica serovar enteritidis (atcc 4931) cells were treated with different concentrations of tsp (1.5, 2.0, and 2.5% [wt/vol]) and compared with (i) cells treated with the same ph as the tsp treatments (ph 10.0, 10.5, and 11.0, respectively) and (ii) cells treated with different concentr ... | 2003 | 12513986 |
| growth dynamics of salmonella enterica strains on alfalfa sprouts and in waste seed irrigation water. | alfalfa sprouts and other seed sprouts have been implicated in numerous outbreaks of salmonellosis. the source of these epidemics appears to have been low-level contamination of seeds by salmonella bacteria that developed into clinically significant populations during the seed germination process. to test the possibility that salmonella enterica strains carry host range determinants that allow them to grow on alfalfa, strains isolated from alfalfa or other sources were surveyed for their ability ... | 2003 | 12514040 |
| inactivation of salmonella enterica serovar enteritidis by ultrasonic waves under pressure at different water activities. | the inactivation of salmonella enterica serovar enteritidis by ultrasonic waves (20 khz; 117- microm wavelength) under pressure (175 kpa) at nonlethal temperatures (manosonication [ms]) and lethal temperatures (manothermosonication [mts]) in media of different water activities has been investigated. heat decimal reduction time values increased 30 times when the water activity was decreased from nearly 1 to 0.96, but the ms resistance was increased only twofold. the inactivation of salmonella ser ... | 2003 | 12514058 |
| subtyping of salmonella enterica serotype enteritidis strains by manual and automated psti-sphi ribotyping. | salmonella enterica subsp. enterica serotype enteritidis is not readily subtyped beyond the level of phage type (pt). a recently developed method for ribotyping of this organism, which uses a mixture of psti and sphi (ps) for restriction of dna (ps ribotyping), has proved useful for further subtyping of a number of pts of this organism, including pt 4. however, it has not been extensively tested with pt 8. in the present study the ps ribotyping method was used to investigate outbreaks of both s. ... | 2003 | 12517821 |
| characterization of the first extended-spectrum beta-lactamase-producing salmonella isolate identified in canada. | a single salmonella enterica serovar typhimurium isolate with an ut2 phage type producing an extended-spectrum beta-lactamase (esbl) was identified in canada in 2000. the isolate harbored two plasmids, one containing a bla(tem-1) gene and the other containing a bla(shv-2a) gene. the esbl gene was located on a 70-kb transferable plasmid which also carried tetracycline and trimethoprim resistance elements. | 2003 | 12517894 |
| the acrb multidrug transporter plays a major role in high-level fluoroquinolone resistance in salmonella enterica serovar typhimurium phage type dt204. | salmonella enterica serovar typhimurium phage type dt204 strains isolated from cattle and animal feed in belgium were characterized for high-level fluoroquinolone resistance mechanisms [mics to enrofloxacin (enr) and ciprofloxacin (cip), 64 and 32 microg/ml, respectively]. these strains isolated during the periods 1991-1994, and in 2000 were clonally related as shown by pulsed-field gel electrophoresis (pfge). selected strains studied carried several mutations in the quinolone target genes, i.e. ... | 2002 | 12523625 |
| phenotypic and genotypic characterization of food animal isolates of salmonella with reduced sensitivity to ciprofloxacin. | reports of nontyphoidal salmonella enterica subsp. enterica showing reduced sensitivity to ciprofloxacin (rsc) have increased rapidly during the past decade. infection in humans with salmonella possessing rsc may compromise the effectiveness of ciprofloxacin therapy. nineteen among 4,357 salmonella strains isolated from food animals in canada from 1998 to 1999 showed rsc; 17 were from turkeys and 2 from chickens. all were resistant to nalidixic acid and sulfisoxazole and possessed rsc at a level ... | 2002 | 12523636 |
| antimicrobial resistance of salmonella serotypes isolated from slaughter-age pigs and environmental samples. | the aim of this study was to determine the antimicrobial resistance patterns of salmonella strains isolated from slaughter-age pigs and environmental samples collected at modern swine raising facilities in brazil. seventeen isolates of six serotypes of salmonella enterica subsp. enterica were isolated out of 1,026 collected samples: salmonella typhimurium (1), salmonella agona (5), salmonella sandiego (5), salmonella rissen (1), salmonella senftenberg (4), and salmonella javiana (1). resistance ... | 2002 | 12523640 |
| regulation of capsule synthesis and cell motility in salmonella enterica by the essential gene igaa. | mutants of salmonella enterica carrying the igaa1 allele, selected as able to overgrow within fibroblast cells in culture, are mucoid and show reduced motility. mucoidy is caused by derepression of wca genes (necessary for capsule synthesis); these genes are regulated by the rcsc/yojn/rcsb phosphorelay system and by the rcsa coregulator. the induction of wca expression in an igaa1 mutant is suppressed by mutations in rcsa and rcsc. reduced motility is caused by lowered expression of the flagella ... | 2002 | 12524328 |
| caenorhabditis elegans innate immune response triggered by salmonella enterica requires intact lps and is mediated by a mapk signaling pathway. | compared to mammals, insects, and plants, relatively little is known about innate immune responses in the nematode caenorhabditis elegans. previous work showed that salmonella enterica serovars cause a persistent infection in the c. elegans intestine that triggers gonadal programmed cell death (pcd) and that c. elegans cell death (ced) mutants are more susceptible to salmonella-mediated killing. to further dissect the role of pcd in c. elegans innate immunity, we identified both c. elegans and s ... | 2003 | 12526744 |
| the interplay between salmonella typhimurium and its macrophage host--what can it teach us about innate immunity? | salmonella enterica sv. typhimurium (s. typhimurium) is a genetically tractable, facultative intracellular pathogen, whose capacity to cause systemic disease in mice depends upon its ability to survive and replicate within macrophages. the identification of salmonella mutants that lack this activity, has provided a tool with which to dissect the mechanisms used by salmonella to establish a permissive niche, and identify host activities which it must overcome in order to achieve this. salmonella ... | 2003 | 12527226 |
| isolated splenic infarction owing to group b salmonella: case report. | the clinical spectrum of extra-intestinal salmonellosis, comprising enteric fever and invasive infections owing to non-typhoidal salmonellae, is well known. we report an otherwise healthy patient with isolated splenic infarction caused by group b salmonella. she was seropositive for the o antigen of salmonella group b and stool cultures were positive for group b salmonellae. after appropriate antimicrobial therapy, her complaints disappeared and microbiological tests for salmonellae became negat ... | 2002 | 12530289 |
| towards new immunotherapies: targeting recombinant cytokines to the immune system using live attenuated salmonella. | we have used salmonella as a delivery system for eukaryotic expression plasmids encoding cytokines, and assessed its capacity to modulate immune responses in different experimental models. plasmids encoding mouse il-4 and il-18 under cytomegalovirus promoter were constructed and transformed into live attenuated salmonella enterica serovar typhi strain cvd 908-htra, and salmonella enterica serovar typhimurium strain sl3261. we have shown that systemic as well as mucosal immunization with such con ... | 2003 | 12531363 |
| animal models paving the way for clinical trials of attenuated salmonella enterica serovar typhi live oral vaccines and live vectors. | attenuated salmonella enterica serovar typhi (s. typhi) strains can serve as safe and effective oral vaccines to prevent typhoid fever and as live vectors to deliver foreign antigens to the immune system, either by the bacteria expressing antigens through prokaryotic expression plasmids or by delivering foreign genes carried on eukaryotic expression systems (dna vaccines). the practical utility of such live vector vaccines relies on achieving a proper balance between minimizing the vaccine's rea ... | 2003 | 12531639 |
| therapeutic efficacy of an e coli strain carrying an ovalbumin allergenic peptide as a fused protein to ompc in a murine model of allergic airway inflammation. | an escherichia coli strain expressing the ovalbumin (ova) 323-329 allergenic peptide on the bacterial surface was evaluated for its ability to reduce the lung inflammatory response in mice allergic to ova. balb/c mice were rendered allergic by means of two intraperitoneal injections of ova suspended in alum 5 days apart, and one intratracheal boost 1 week later. the mice were then treated with two intranasal, 1 week apart, doses of 4x10(9) e. coli-uh302 transformed with plasmids pst13 or pst13-o ... | 2003 | 12531657 |
| household contamination with salmonella enterica. | household contamination with salmonella enterica increases when occupational exposure exists (cattle farms with known salmonellosis in cattle, a salmonella research laboratory, or a veterinary clinic experiencing an outbreak of salmonellosis). fifteen of 55 (27.2%) vacuum cleaner bags from households with occupational exposure to s. enterica were positive versus 1 of 24 (4.2%) without known exposure. use of a carpet cleaner and several cleaners/disinfectants reduced, but failed to eliminate, s. ... | 2003 | 12533294 |
| 1-methylguanosine-deficient trna of salmonella enterica serovar typhimurium affects thiamine metabolism. | in salmonella enterica serovar typhimurium a mutation in the purf gene encoding the first enzyme in the purine pathway blocks, besides the synthesis of purine, the synthesis of thiamine when glucose is used as the carbon source. on carbon sources other than glucose, a purf mutant does not require thiamine, since the alternative pyrimidine biosynthetic (apb) pathway is activated. this pathway feeds into the purine pathway just after the purf biosynthetic step and upstream of the intermediate 4-am ... | 2003 | 12533450 |
| characterization of srga, a salmonella enterica serovar typhimurium virulence plasmid-encoded paralogue of the disulfide oxidoreductase dsba, essential for biogenesis of plasmid-encoded fimbriae. | disulfide oxidoreductases are viewed as foldases that help to maintain proteins on productive folding pathways by enhancing the rate of protein folding through the catalytic incorporation of disulfide bonds. srga, encoded on the virulence plasmid pstsr100 of salmonella enterica serovar typhimurium and located downstream of the plasmid-borne fimbrial operon, is a disulfide oxidoreductase. sequence analysis indicates that srga is similar to dsba from, for example, escherichia coli, but not as high ... | 2003 | 12533475 |
| traffic at the tmrna gene. | a partial screen for genetic elements integrated into completely sequenced bacterial genomes shows more significant bias in specificity for the tmrna gene (ssra) than for any type of trna gene. horizontal gene transfer, a major avenue of bacterial evolution, was assessed by focusing on elements using this single attachment locus. diverse elements use ssra; among enterobacteria alone, at least four different integrase subfamilies have independently evolved specificity for ssra, and almost every s ... | 2003 | 12533482 |
| the high-pathogenicity island is absent in human pathogens of salmonella enterica subspecies i but present in isolates of subspecies iii and vi. | in this study we tested 74 salmonella strains of all eight salmonella groups and were able to demonstrate the presence of two high-pathogenicity island types in strains of salmonella groups iiia, iiib, and vi. most high-pathogenicity island-positive isolates produced yersiniabactin under iron-limited conditions and were positive for the high-molecular-weight proteins hmwp1 and hmwp2. | 2003 | 12533488 |
| structural and biological characterization of highly purified hepta-acyl lipid a present in the lipopolysaccharide of the salmonella enterica sv. minnesota re deep rough mutant strain r595. | one major component of the salmonella enterica sv. minnesota re deep rough mutant (strain r595) lipopolysaccharide is hepta-acyl lipid a (la(hepta)). in a recent publication [tanamoto k-i, azumi s. salmonella-type heptaacylated lipid a is inactive and acts as an antagonist of lipopolysaccharide action on human line cells. j immunol 2000; 164: 3149-3156] the corresponding synthetic hepta-acyl lipid a (compound 516) was reported to be agonistically inactive but to rather suppress pro-inflammatory ... | 2002 | 12537693 |
| comparison of sublethal injury induced in salmonella enterica serovar typhimurium by heat and by different nonthermal treatments. | we have studied sublethal injury in salmonella enterica serovar typhimurium caused by mild heat and by different emerging nonthermal food preservation treatments, i.e., high-pressure homogenization, high hydrostatic pressure, pulsed white light, and pulsed electric field. sublethal injury was determined by plating on different selective media, i.e., tryptic soy agar (tsa) plus 3% nacl, tsa adjusted to ph 5.5, and violet red bile glucose agar. for each inactivation technique, at least five treatm ... | 2003 | 12540178 |
| molecular and phenotypic analysis of the cs54 island of salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants. | the shda gene is carried on a 25-kb genetic island at centisome 54 (cs54 island) of the salmonella enterica serotype typhimurium chromosome. in addition to shda, the cs54 island of salmonella serotype typhimurium strain lt2 contains four open reading frames designated rata, ratb, sivi, and sivh. dna hybridization analysis revealed that the cs54 island is comprised of two regions with distinct phylogenetic distribution within the genus salmonella. homologues of shda and ratb were detected only in ... | 2003 | 12540539 |
| lon, a stress-induced atp-dependent protease, is critically important for systemic salmonella enterica serovar typhimurium infection of mice. | studies on the pathogenesis of salmonella enterica serovar typhimurium infections in mice have revealed the presence of two prominent virulence characteristics-the invasion of the nonphagocytic cells to penetrate the intestinal epithelium and the proliferation within host phagocytic cells to cause a systemic spread and the colonization of host organs. we have recently demonstrated that the atp-dependent lon protease of s. enterica serovar typhimurium negatively regulates the efficiency of invasi ... | 2003 | 12540547 |
| disruption of epithelial barrier integrity by salmonella enterica serovar typhimurium requires geranylgeranylated proteins. | epithelial cells that line the human intestinal mucosa constitute the initial sites of host invasion by bacterial pathogens. a number of bacteria, such as salmonella and yersinia spp., have been shown to disrupt the integrity of the epithelial barrier, although little is known about the mechanisms underlying that effect. we found that polarized mdck-1 epithelial cells infected with invasive salmonella enterica serovar typhimurium sl1344 exhibited marked changes in f-actin organization, an increa ... | 2003 | 12540569 |
| characterization of a highly attenuated salmonella enterica serovar typhimurium mutant strain. | salmonella enterica serovar typhimurium atcc 13311 is virulent at a dose as low as 10(2) colony-forming units when administered intraperitoneally to balb/c mice. in order to develop highly attenuated mutant strain through the combination of 2 phenotypically attenuated markers, we constructed a number of amino acid requiring auxotrophic strains of s. enterica serovar typhimurium by means of uv-induced mutations. one of them, strain ndmc-b1, was highly attenuated for mice, with an ld50-value of 6 ... | 2002 | 12542248 |
| in vivo acquisition of ceftriaxone resistance in salmonella enterica serotype anatum. | the emergence of resistance to antimicrobial agents within the salmonellas is a worldwide and severe problem. a case of treatment failure due to the emergence of resistance to ceftriaxone in salmonella enterica serotype anatum was studied. s. enterica serotype anatum and escherichia coli, both of which are susceptible to ceftriaxone, were initially isolated from a diabetic patient hospitalized for the treatment of wound and urinary tract infections. resistant s. enterica serotype anatum and e. c ... | 2003 | 12543660 |
| salmonella enterica serovar typhimurium bla(per-1)-carrying plasmid psti1 encodes an extended-spectrum aminoglycoside 6'-n-acetyltransferase of type ib. | we have studied the aminoglycoside resistance gene, which confers high levels of resistance to both amikacin and gentamicin, that is carried by plasmid psti1 in the per-1 beta-lactamase-producing strain of salmonella enterica serovar typhimurium previously isolated in turkey. this gene, called aac(6')-ib(11), was found in a class 1 integron and codes for a protein of 188 amino acids, a fusion product between the n-terminal moiety (8 amino acids) of the signal peptide of the beta-lactamase oxa-1 ... | 2003 | 12543680 |
| characterization of class i integrons in clinical strains of salmonella enterica subsp. enterica serovars typhimurium and enteritidis from norwegian hospitals. | the characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens salmonella enterica subsp. enterica serovars typhimurium (s. typhimurium) and enteritidis (s. enteritidis) from norwegian hospitals were performed. integrons were found in 64 of 66 s. typhimurium isolates (97 %) and in 20 of 90 s. enteritidis isolates (22.2 %) with the following sizes; 650, 1000, 1200, 1500, 1600, 1700, 2000 and 2100 bp. the integrons were furth ... | 2003 | 12543920 |
| a statewide outbreak of salmonella bovismorbificans phage type 32 infection in queensland. | between 30 may and 1 june 2001, 10 cases of salmonella bovismorbificans infection were reported to public health services, queensland health. investigations included enhanced surveillance, case interviews, a matched case control study, environmental audit and microbiological testing of faecal isolates (phage typing) and implicated food products. forty-one cases of s. bovismorbificans infection were detected, 36 cases were phage type 32. a matched case control study identified that illness was as ... | 2002 | 12549525 |
| mucosal priming of simian immunodeficiency virus-specific cytotoxic t-lymphocyte responses in rhesus macaques by the salmonella type iii secretion antigen delivery system. | nearly all human immunodeficiency virus (hiv) infections are acquired mucosally, and the gut-associated lymphoid tissues are important sites for early virus replication. thus, vaccine strategies designed to prime virus-specific cytotoxic t lymphocyte (ctl) responses that home to mucosal compartments may be particularly effective at preventing or containing hiv infection. the salmonella type iii secretion system has been shown to be an effective approach for stimulating mucosal ctl responses in m ... | 2003 | 12551977 |
| comparison of salmonella enterica serovar abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting. | equine paratyphoid is caused by salmonella enterica serovar abortusequi, and manifests mainly as abortion in the mare. we compared s. abortusequi strains isolated in japan and other countries using pulsed-field gel electrophoresis (pfge) and fluorescent amplified-fragment length polymorphism (faflp) analysis. pfge analysis of s. abortusequi strains gave 21-27 fragments ranging in size from 33 to 602kb. although two pfge profiles were observed among the 20 s. abortusequi isolates in japan, the re ... | 2003 | 12554106 |
| vaccination against type f botulinum toxin using attenuated salmonella enterica var typhimurium strains expressing the bont/f h(c) fragment. | the utility of the htra, pagc and nirb promoters to direct the expression of the carboxy-terminal (h(c)) fragment of botulinum toxin f (fh(c)) in salmonella enterica var typhimurium has been evaluated. only low levels of serum antibody were induced after immunisation, and some protection against botulinum toxin type f was demonstrated after oral immunisation of mice with two doses of any of these recombinant salmonella. immunisation with two doses of recombinant salmonella expressing fh(c) from ... | 2003 | 12559779 |
| 2-aminopurine allows interspecies recombination by a reversible inactivation of the escherichia coli mismatch repair system. | 2-aminopurine treatment of escherichia coli induces a reversible phenotype of dna mismatch repair deficiency. this transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a salmonella enterica serovar typhimurium hfr donor. this method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes. | 2003 | 12562818 |
| detection of hila gene sequences in serovars of salmonella enterica subspecies enterica. | hila gene promoter, component of the salmonella pathogenicity island 1, has been found in salmonella serovar typhimurium, being important for the regulation of type iii secretion apparatus genes. we detected hila gene sequences in salmonella serovars typhi, enteritidis, choleraesuis, paratyphi a and b, and pullorum, by polymerase chain reaction (pcr) and hybridization techniques. the primers to carry out pcr were designed according to hila sequence. a low stringency hybridization with the probe ... | 2003 | 12563483 |
| collaborative investigation of an outbreak of salmonella enterica serotype newport in england and wales in 2001 associated with ready-to-eat salad vegetables. | in june 2001, as part of a microbiological study of bagged, ready-to-eat salad products, salmonella enterica serotype newport was isolated from a sample of pre-packed green salad distributed by a major supermarket retailer. the strain was characterised by phage typing, plasmid profile typing and pulsed-field gel electrophoresis. other isolates of s. newport from cases of human infection in england and wales in the first six months of 2001 were similarly characterised. of 60 strains from cases of ... | 2002 | 12564245 |
| osteomyelitis associated with salmonella enterica ss arizonae in a colony of ridgenose rattlesnakes (crotalus willardi). | the identification of three arizona ridgenose rattlesnakes (crotalus willardi) with salmonella arizonae-associated osteomyelitis led to a 5-yr prospective study of radiographic signs and salmonella intestinal carriage rates in a 19-member colony of this rattlesnake species. ventrodorsal radiographs were performed and cloacal swabs were cultured for salmonella spp. annually. ten snakes survived the 5-yr period, with six of them remaining free of bony lesions. three snakes that had no bony lesions ... | 2002 | 12564525 |
| enteric fever in mumbai, india: the good news and the bad news. | 2003 | 12567317 | |
| molecular analyses of salmonella enterica isolates from fish feed factories and fish feed ingredients. | isolates of the most commonly observed salmonella serovars in norwegian fish feed factories from 1998 to 2000 (salmonella enterica serovar agona, s. enterica serovar montevideo, s. enterica serovar senftenberg, and s. enterica serovar kentucky) were studied by pulsed-field gel electrophoresis (pfge) and plasmid profile analysis and compared to isolates of the same serovars from fish feed ingredients, humans, and other sources (a total of 112 isolates). within each serovar, a variety of distinct ... | 2003 | 12571031 |
| [antimicrobial resistance of clinical strains of salmonella enterica isolated in zaragoza]. | in order to identify any changes in the incidence of salmonella enterica serotypes and their resistance to a variety of antimicrobial agents, we conducted a retrospective study of all the strains isolated from stool samples at hospital clínico universitario lozano blesa in zaragoza from 1997 to 2000. we observed an increase in the number of isolates of salmonella and campylobacter and a decrease in other enteropathogens. enteritidis was the most frequently isolated serotype (55.2%), showing an i ... | 2002 | 12582444 |
| growth and colonization suppression of salmonella enterica serovar hadar in vitro and in vivo. | growth suppression in salmonella enterica serovar hadar (s. hadar) was investigated, in vitro under strict anaerobiosis and in vivo in the intestine of the day-old chicken. stationary-phase cultures of 20 s. hadar field strains were tested against each other for growth suppression activity by their ability to suppress the multiplication of low counts of minority cultures inoculated into them as nalidixic acid-resistant mutants. all strains showed profound growth suppression. four s. hadar strain ... | 2003 | 12583908 |
| [characterization of a new serotype of salmonella enterica subsp. enterica: serotype bata]. | 2003 | 12586026 | |
| phenotypic evidence for inducible multiple antimicrobial resistance in salmonella choleraesuis. | multiple antimicrobial resistance (mar) in salmonella choleraesuis is becoming a major concern. it has been demonstrated that a mar phenotype can be induced in escherichia coli and other members of the enterobacteriaceae by exposing the isolates to salicylates, various antimicrobials, or organic solvents used to combat and control bacterial infection. therefore the purpose of the present study was to determine whether this mara-associated mar-phenotype is inducible in s. choleraesuis. isolates u ... | 2003 | 12586413 |
| identification of glycosphingolipid binding sites for sef21-fimbriated salmonella enterica serovar enteritidis in chicken oviductal mucosa. | in order to clarify the presence of glycosphingolipids (gsls) receptors for salmonella enterica serovar enteritidis with sef21 fimbriae, we analyzed neutral gsls and gangliosides from chicken oviductal mucosa and investigated the binding of bacteria to neutral gsls and gangliosides. five types of neutral gsls, designated as n-1 to n-5, and two types of gangliosides, designated as g-1 and g-2, were identified on the thin-layer chromatography (tlc) plates. in the bacterial binding assay on tlc, th ... | 2003 | 12591208 |
| overexpression of the waaz gene leads to modification of the structure of the inner core region of escherichia coli lipopolysaccharide, truncation of the outer core, and reduction of the amount of o polysaccharide on the cell surface. | the waa gene cluster is responsible for the biosynthesis of the lipopolysaccharide (lps) core region in escherichia coli and salmonella: homologs of the waaz gene product are encoded by the waa gene clusters of salmonella enterica and e. coli strains with the k-12 and r2 core types. overexpression of waaz in e. coli and s. enterica led to a modified lps structure showing core truncations and (where relevant) to a reduction in the amount of o-polysaccharide side chains. mass spectrometry and nucl ... | 2003 | 12591884 |
| concomitant induction of cd4+ and cd8+ t cell responses in volunteers immunized with salmonella enterica serovar typhi strain cvd 908-htra. | type 1 cell-mediated immunity might play an important role in protection from typhoid fever. we evaluated whether immunization with salmonella enterica serovar typhi (s. typhi) strain cvd 908-htra (a delta aroc delta arod delta htra mutant), a leading live oral typhoid vaccine candidate, elicits specific cd4(+) and cd8(+) s. typhi immune responses. potent ctl responses and ifn-gamma secretion by cd8(+) t cells were detected following immunization with cvd 908-htra in high (4.5 x 10(8) cfu) and l ... | 2003 | 12594304 |
| enteric salmonella infection inhibits paneth cell antimicrobial peptide expression. | paneth cells, highly secretory epithelial cells found at the bases of small intestinal crypts, release a variety of microbicidal molecules, including alpha-defensins and lysozyme. the secretion of antimicrobials by paneth cells is thought to be important in mucosal host defense against invasion by enteric pathogens. we explored whether enteric pathogens can interfere with this arm of defense. we found that oral inoculation of mice with wild-type salmonella enterica serovar typhimurium decreases ... | 2003 | 12595421 |
| allelic variation in tlr4 is linked to susceptibility to salmonella enterica serovar typhimurium infection in chickens. | toll-like receptor 4 (tlr4) is part of a group of evolutionarily conserved pattern recognition receptors involved in the activation of the immune system in response to various pathogens and in the innate defense against infection. we describe here the cloning and characterization of the avian orthologue of mammalian tlr4. chicken tlr4 encodes a 843-amino-acid protein that contains a leucine-rich repeat extracellular domain, a short transmembrane domain typical of type i transmembrane proteins, a ... | 2003 | 12595422 |
| the shufflon of salmonella enterica serovar typhi regulates type ivb pilus-mediated bacterial self-association. | previously, it was shown that type ivb pili encoded by the salmonella enterica serovar typhi pil operon are used to facilitate bacterial entry into human intestinal epithelial cells in vitro and that such entry is inhibited by purified prepilin (pre-pils) protein (x.-l. zhang, i. s. m. tsui, c. m. c. yip, a. w. y. fung, d. k.-h. wong, x. dai, y. yang, j. hackett, and c. morris, infect. immun. 68:3067-3073, 2000). the pil operon concludes with a simple shufflon, and a recombinase gene product (rc ... | 2003 | 12595425 |
| functional cd40 expression induced following bacterial infection of mouse and human osteoblasts. | bacterially induced bone infections often result in significant local inflammatory responses which are coupled with loss of bone. however, the mechanisms necessary for the protective host response, or those responsible for pathogen-induced bone loss, are not clear. recent evidence demonstrates that bacterially infected osteoblasts secrete chemokines and cytokines, suggesting that these cells may have an unappreciated role in supporting localized inflammation. in this study, mouse and human osteo ... | 2003 | 12595434 |
| hile interacts with hild and negatively regulates hila transcription and expression of the salmonella enterica serovar typhimurium invasive phenotype. | the ability of salmonella enterica serovar typhimurium to traverse the intestinal mucosa of a host is an important step in its ability to initiate gastrointestinal disease. the majority of the genes required for this invasive characteristic are encoded on salmonella pathogenicity island 1 (spi1), and their expression is controlled by the transcriptional activator hila, a member of the ompr/toxr family of proteins. a variety of genes (hilc, hild, fis, sira/bara, csrab, phob, fadd, envz/ompr, fliz ... | 2003 | 12595445 |
| purification, crystallization and preliminary x-ray diffraction studies of recombinant class a non-specific acid phosphatase of salmonella typhimurium. | the phon gene of salmonella enterica sv. typhimurium strain md6001 was cloned in the multicopy plasmid pbluescript sk(-). the nucleotide sequence of the cloned gene differs from the corresponding s. typhimurium lt2 sequence at 23 residues, leading to 15 amino-acid differences, but was very close to the s. typhi phon sequence (only three nucleotide and two amino-acid differences). the recombinant phon protein was purified to homogeneity. two forms of crystals were harvested from a single crystall ... | 2003 | 12595712 |
| crystallization of the membrane-containing bacteriophage prd1 in quartz capillaries by vapour diffusion. | crystals of bacteriophage prd1, a virus containing an internal lipid bilayer, have been grown in thin-walled quartz capillary tubes by vapour diffusion as a means of eliminating mechanical handling of the crystals during data collection. it has been found that the addition of polyethylene glycol 20 000 (peg 20k) to the mother liquor that bathes the crystals allows far higher resolution diffraction intensities to be observed. growing and treating the crystals in this way has produced a small numb ... | 2003 | 12595719 |
| molecular epidemiology of salmonella enterica serovar enteritidis isolated in taiwan. | incidence of salmonella enterica serovar enteritidis infection seems to be on the rise in taiwan, and therefore, the characteristics of the isolate, including genotypes, were epidemiologically investigated. of the 71 clinical strains isolated in 1997-1999, 61 (86%) remained susceptible to the eight antibiotics tested, while the remaining ten, eight of which were isolated in 1999, were resistant to one to three of the agents including three multiply resistant strains. the majority, 69 or 97% of t ... | 2002 | 12597357 |
| development of a mucosal complex vaccine against oral salmonella infection in mice. | we examined the immunogenicity of a salmonella enterica complex vaccine (cv), consisting of flagellin and polysome purified from serotype typhimurium lt2. cv plus cholera toxin (ct), in three oral doses given at 7-day intervals, conferred complete protection on c57bl/6 mice against lethal oral infection with a wild-type strain. it elicited mucosal iga > igg2a > igg1 and systemic igg2a > igg1 > iga antibodies to flagellin and polysome, and delayed footpad response (dfr) to both antigens. in peyer ... | 2002 | 12597365 |
| filament formation by salmonella spp. inoculated into liquid food matrices at refrigeration temperatures, and growth patterns when warmed. | in this study, the formation of multicellular filamentous salmonella cells in response to low temperatures was investigated by using isolates of salmonella enterica serovar enteritidis pt4 and s. enterica serovar typhimurium dt104 as the inocula. the formation of filamentous cells in two liquid food matrices at the recommended maximum temperature for refrigeration (8 degrees c) was monitored and compared with that in tryptone soya broth. giemsa staining was performed to locate nuclear material w ... | 2003 | 12597479 |
| suppression of salmonella growth by wild-type and large-plaque variants of bacteriophage felix o1 in liquid culture and on chicken frankfurters. | the bacteriophage felix o1, a member of myoviridae, is specific for, and possesses a broad host range within, the genus salmonella. this work explores a felix o1 phage-based intervention for salmonella enterica serotype typhimurium dt104 that is potentially applicable at several stages of animal production and processing. a variant of felix o1 was obtained that produces a larger, clearer plaque phenotype (lp) on salmonella typhi than wild-type felix o1 (wt) does, not unlike r mutants of phage t4 ... | 2003 | 12597480 |
| hsp60 specific antibodies in egg yolks from laying hens naturally infected with salmonella enterica subspecies enterica serovar enteritidis. | heat shock protein (hsp) 60 of salmonella appears to be involved in pathogenesis of infectious processes and host immune responses. eggs of laying hens from two salmonella enteritidis naturally infected flocks (i--acute outbreak of infection; ii--occasional bacteria excretion) and one control flock (iii) were tested for the presence of yolk antibodies (igy) against hsp60 by applying enzyme-linked immunosorbent assay (elisa). the levels of specific immunoglobulins were related to those against li ... | 2003 | 12602685 |
| life-threatening infantile diarrhea from fluoroquinolone-resistant salmonella enterica typhimurium with mutations in both gyra and parc. | salmonella typhimurium dt12, isolated from a 35-day-old infant with diarrhea, was highly resistant to ampicillin, tetracycline, chloramphenicol, streptomycin, gentamycin, sulfamethoxazole/trimethoprim, nalidixic acid, and fluoroquinolones. the patient responded to antibiotic therapy with fosfomycin. multidrug-resistance may become prevalent in salmonella infections in japan, as shown in this first case of a patient infected with fluoroquinolone-resistant salmonella. | 2003 | 12604000 |
| a new inhibitor of the transcription-termination factor rho. | in this study we describe bi-k0058, a new inhibitor of the transcription-termination factor rho belonging to a different chemical class from bicyclomycin, the only known antibiotic acting on rho. bi-k0058 inhibits the poly(c)-dependent atpase activity of rho with an ic(50) of 25 microm as well as in vitro transcription-termination of two natural substrates, the salmonella enterica hisg cistron and the f1 phage intergenic region. bi-k0058 does not affect photolabeling of rho by atp. the results o ... | 2003 | 12604334 |
| imipenem resistance in salmonella enterica serovar wien related to porin loss and cmy-4 beta-lactamase production. | two multidrug-resistant salmonella enterica serovar wien strains (sw468 and sw1107) were isolated in 2001 in tunis. both strains produced the beta-lactamases tem-1, shv-2a, and cmy-4, whereas strain sw1107 also produced the beta-lactamase ctx-m-3. the imipenem-resistant strain (sw468) was totally devoid of the ompf-immunorelated porin. imipenem resistance was shown as being related to porin loss and cmy-4 beta-lactamase production. | 2003 | 12604564 |
| phylogenetic analysis of salmonella enterica serovar enteritidis isolated from food poisoning outbreaks and sporadic infections in 2001-2002 in hyogo prefecture: existent of predominant genotypes in the epidemic. | 2002 | 12606832 | |
| subtracted restriction fingerprinting--a tool for bacterial genome typing. | reproducible, discriminative, high-throughput methods are required for the identification of bacterial strains and isolates in a clinical environment. a new molecular typing method for bacteria was developed and tested on salmonella and e. coli species. the technique is called subtracted restriction fingerprinting and is based on double restriction enzyme digestion of genomic dna followed by end labeling. the "detection" enzyme produces ttaa overhangs that are filled in with digoxigenated nucleo ... | 2003 | 12613253 |
| short-chain fatty acid activation by acyl-coenzyme a synthetases requires sir2 protein function in salmonella enterica and saccharomyces cerevisiae. | sir2 proteins have nad(+)-dependent histone deacetylase activity, but no metabolic role has been assigned to any of these proteins. in salmonella enterica, sir2 function was required for activity of the acetyl-coa synthetase (acs) enzyme. a greater than two orders of magnitude increase in the specific activity of acs enzyme synthesized by a sirtuin-deficient strain was measured after treatment with homogeneous s. enterica sir2 protein. human sir2a and yeast sir2 proteins restored growth of sir2- ... | 2003 | 12618394 |
| mutational analysis of the residue at position 48 in the salmonella enterica serovar typhimurium phoq sensor kinase. | the phop/phoq two-component regulatory system of salmonella enterica serovar typhimurium plays an essential role in controlling virulence by mediating the adaptation to mg(2+) depletion. the pho-24 allele of phoq harbors a single amino acid substitution (t48i) in the periplasmic domain of the phoq histidine kinase sensor. this mutation has been shown to increase net phosphorylation of the phop response regulator. we analyzed the effect on signaling by phop/phoq of various amino acid substitution ... | 2003 | 12618457 |
| histopathological study of rabbit intestinal mucosa infected with a hybrid strain of shigella dysenteriae 1 carrying lps biosynthesis genes of salmonella enterica serovar typhimurium. | the rfb gene cluster and the rfc gene of salmonella enterica were introduced earlier into an invasive shigella dysenteriae 1 strain by triparental cross. antiserum was raised in rabbit against lipopolysaccharide isolated from the hybrid strain. both the hybrid and the invasive s. dysenteriae 1 strain were found to have a titer of 1:2560 while for s. enterica, it was 1:640. ligated ileal loops were prepared in rabbit, which were inoculated with 10(8) cfu ml(-1) each of the hybrid strain, and inva ... | 2003 | 12620623 |
| survival of bacterial indicator species and bacteriophages after thermal treatment of sludge and sewage. | the inactivation of naturally occurring bacterial indicators and bacteriophages by thermal treatment of a dewatered sludge and raw sewage was studied. the sludge was heated at 80 degrees c, and the sewage was heated at 60 degrees c. in both cases phages were significantly more resistant to thermal inactivation than bacterial indicators, with the exception of spores of sulfite-reducing clostridia. somatic coliphages and phages infecting bacteroides fragilis were significantly more resistant than ... | 2003 | 12620828 |
| kinetics and strain specificity of rhizosphere and endophytic colonization by enteric bacteria on seedlings of medicago sativa and medicago truncatula. | the presence of human-pathogenic, enteric bacteria on the surface and in the interior of raw produce is a significant health concern. several aspects of the biology of the interaction between these bacteria and alfalfa (medicago sativa) seedlings are addressed here. a collection of enteric bacteria associated with alfalfa sprout contaminations, along with escherichia coli k-12, salmonella enterica serotype typhimurium strain atcc 14028, and an endophyte of maize, klebsiella pneumoniae 342, were ... | 2003 | 12620870 |
| epidemiology of tetracycline resistance determinants in shigella spp. and enteroinvasive escherichia coli: characterization and dissemination of tet(a)-1. | to make a comprehensive study of tetracycline resistance determinant distribution in the genus shigella, a collection of 577 clinical isolates of shigella spp. and enteroinvasive escherichia coli (eiec) from a variety of geographical locations was screened to identify tetracycline-resistant strains. the 459 tetracycline-resistant isolates identified were then screened by pcr analysis to determine the distribution in these strains of tetracycline efflux resistance determinants belonging to classe ... | 2003 | 12624025 |
| csgd, a regulator of curli and cellulose synthesis, also regulates serine hydroxymethyltransferase synthesis in escherichia coli k-12. | the homologous csgd and agfd proteins are members of the fixj/uhpa/luxr family and are proposed to regulate curli (thin aggregative fibres) and cellulose production by escherichia coli and salmonella enterica serovar typhimurium, respectively. a plasmid containing part of the csgd gene was isolated during a screen for multicopy suppressors of glycine auxotrophy caused by deleting the fola gene in e. coli. the sequence of the plasmid suggests it encodes a chimaeric protein. plasmids containing th ... | 2003 | 12624214 |
| the 1.75 a crystal structure of acetyl-coa synthetase bound to adenosine-5'-propylphosphate and coenzyme a. | acetyl-coenzyme a synthetase catalyzes the two-step synthesis of acetyl-coa from acetate, atp, and coa and belongs to a family of adenylate-forming enzymes that generate an acyl-amp intermediate. this family includes other acyl- and aryl-coa synthetases, firefly luciferase, and the adenylation domains of the modular nonribosomal peptide synthetases. we have determined the x-ray crystal structure of acetyl-coa synthetase complexed with adenosine-5'-propylphosphate and coa. the structure identifie ... | 2003 | 12627952 |
| adaptive responses of salmonella enterica serovar typhimurium dt104 and other s. typhimurium strains and escherichia coli o157 to low ph environments. | cattle are a known main reservoir for acid-resistant escherichia coli o157 and salmonella enterica serovar typhimurium dt104. we studied the response of s. typhimurium dt104 to extreme low ph environments and compared their response to that of acid-resistant e. coli o157 and other s. typhimurium phage types. | 2003 | 12631198 |
| construction and evaluation of a eukaryotic expression plasmid for stable delivery using attenuated salmonella. | an approach to enhancing the stability of eukaryotic expression plasmids for delivery using attenuated salmonella has been evaluated. the expression apparatus and beta-galactosidase gene from the expression plasmid, pcmvbeta, was cloned into the low copy number plasmid plg339. the resulting construct, plgbetagal, was shown to have a lower copy number than pcmvbeta in salmonella enterica var typhimurium aroa strain sl7207. furthermore, beta-galactosidase-specific antibody was induced in mice foll ... | 2003 | 12631472 |
| systemic and enteric colonization of pigs by a hila signature-tagged mutant of salmonella choleraesuis. | although host adapted to pigs, salmonella enterica serovar choleraesuis (s. choleraesuis) can induce a virulent foodborne salmonellosis in humans. to directly investigate virulence factors of s. choleraesuis, we extended the functional genomics approach of signature-tagged mutagenesis to s. choleraesuis and pigs. when a test pool of 45 randomly signature-tagged null mutants was inoculated orally and intraperitoneally in pigs, one of the mutants that failed to colonize by either route was tagged ... | 2003 | 12631476 |
| endemic presence of salmonella enterica serotype cerro in southern italy. | molecular typing of salmonella strains isolated between 1997 and 1999 in southern italy and carried out by the southern italy centre for enteric pathogens, has shown a high frequency of salmonella enterica serotype cerro. this serotype is extremely rare i | 2000 | 12631854 |
| occurrence of salmonella enterica serovar dublin in austria. | in austria, salmonella enterica subsp. enterica serovar dublin, a bovine-adapted serovar, rarely causes infections in humans. in 2000, austria was within the european mean with an incidence of 0.1 per million inhabitants. our data show that the vast majority of all serovar dublin infections (human and non-human) can be traced epidemiologically to two districts in the tyrol. this concentration of cases can be explained by a particularly traditional aspect of cattle farming in this area, the alpin ... | 2002 | 12631940 |
| evolution of antibiotic resistance of non-typhoidal salmonellae in greece during 1990-97. | susceptibility to 15 antibiotics was determined in 1548 non-typhoidal salmonella strains isolated in greece from l990 to l997. the overall prevalence of resistance of both salmonella enterica serotype enteritidis and salmonella enterica serotype typhimurium increased during the first years of the study. a decrease was observed from 1996, especially for s. enteritidis, which showed the highest overall antibiotic resistance. s. typhimurium was the serotype with the highest multiresistance to antib ... | 2001 | 12631956 |
| surveillance of antimicrobial resistance in denmark. | recent data of the danish integrated antimicrobial resistance monitoring and research programme (danmap) show that, in denmark, resistance levels among salmonella enterica are modest and that resistance in escherichia coli isolates causing disease in anim | 2000 | 12631958 |
| salmonella enterica serotype oranienburg infections associated with consumption of locally produced tyrolean cheese. | sixteen culture confirmed cases of enteric infection with salmonella enterica serotype oranienburg were detected between august 10 and september 29 1999 in tyrol (austria). ten of them suffered bloody diarrhoea and six were asymptomatic carriers. intervie | 2000 | 12631963 |
| investigation of human infections with salmonella enterica serovar java in scotland and possible association with imported poultry. | pfge analysis of s. java strains (29 from humans, 30 from poultry meat) showed two major clusters. all isolates from poultry imported from the netherlands belonged to cluster a, which also comprised 10 human isolates. thirty-one of the 37 isolates in this cluster had an identical javx1 pattern, similar to the x8 profile of a particular s. java clone predominant in poultry production in several european countries. cluster b comprised 19 human isolates and two poultry isolates of unknown origin. t ... | 2003 | 12631973 |
| antimicrobial drug resistance in isolates of salmonella enterica from cases of salmonellosis in humans in europe in 2000: results of international multi-centre surveillance. | the enter-net surveillance system received results of antimicrobial sensitivity tests for isolates from over 27 000 cases of human salmonellosis in 2000 in 10 european countries. almost 40% of isolates were resistant to at least one antimicrobial, with 18% multiresistant. resistance to ampicillin, streptomycin, sulphonamides and tetracyclines was common, with over 20% of isolates resistant to at least one of these antimicrobials. clinical resistance to ciprofloxacin was rare, with only 0.5% of i ... | 2003 | 12631974 |
| monocytic activation of protein tyrosine kinase, protein kinase a and protein kinase c induced by porins isolated from salmonella enterica serovar typhimurium. | in the present study a monocytic cell line, u937, was used to investigate the possible involvement of protein tyrosine kinases (nt-ptks), protein kinase a (pka) and protein kinase c (pkc) in cell signaling pathways following salmonella enterica serovar typhimurium porin stimulation. | 2003 | 12634073 |
| interactions of salmonella enterica subsp. enterica serovar muenchen with intestinal explants of the turtle trachemys scripta scripta. | salmonella infections in reptiles, in contrast to those in birds and mammals, are limited to the intestinal tract. in this study, interactions of a strain of salmonella enterica subsp. enterica serovar muenchen (seem) with intestinal explants of the turtle trachemys scripta scripta were examined by scanning electron microscopy (sem). adhesion and invasion in the chelonian intestinal explants at 30 degrees c and 37 degrees c were evaluated quantitatively. for purposes of comparison, the invasive ... | 2003 | 12634088 |
| evaluation of guinea pig model for experimental salmonella serovar abortusequi infection in reference to infertility. | the present study conclusively revealed the role for salmonella enterica subspecies enterica serovar abortusequi in conception failure. none of the 12 guinea pigs conceived when orally exposed to sublethal dose of the pathogen during breeding, while 66.67% of animals in control group were found pregnant during same period of observation under similar conditions. salmonella carrier animals also had drastic reduction in conception rate (16.67%). during mid pregnancy, s. abortusequi exposure to gui ... | 2002 | 12635699 |
| subtyping of pathogenic escherichia coli strains using flagellar (h)-antigens: serotyping versus flic polymorphisms. | serotyping of o- and h-antigens is regarded as the gold standard in classification of e. coli for taxonomic and epidemiological purposes similar to the kaufmann-white scheme for salmonella enterica. molecular methods to replace or to support the serotyping have been applied recently. using the molecular polymorphism of the flagella (h-antigen) gene flic, more than 220 e. coli strains derived from the e. coli reference collection for o- and h-antigens (the international escherichia and klebsiella ... | 2003 | 12635930 |
| evaluation of the 1-2 test for detecting salmonella in swine feces. | the 1-2 test is a rapid culture test for the detection of motile salmonella. the aim of this study was to evaluate the 1-2 test for its ability to detect salmonella in swine feces following preenrichment and selective enrichment. pooled pen fecal samples (n = 118) and pig rectal swabs (n = 51) were cultured for salmonella by the 1-2 test, which was compared with the standard isolation protocol currently used in our laboratory. in addition, pen fecal samples known to be free of salmonella were sp ... | 2003 | 12636313 |
| emergence of ceftriaxone-resistant salmonella isolates and rapid spread of plasmid-encoded cmy-2-like cephalosporinase, taiwan. | of 384 salmonella isolates collected from 1997 to 2000 in a university hospital in taiwan, six ceftriaxone-resistant isolates of salmonella enterica serovar typhimurium were found in two patients in 2000. the resistance determinants were on conjugative plasmids that encoded a cmy-2-like cephalosporinase. during the study period, the proportion of cmy-2-like enzyme producers among escherichia coli increased rapidly from 0.2% in early 1999 to >4.0% in late 2000. klebsiella pneumoniae isolates prod ... | 2003 | 12643826 |