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subgingival microbiota in healthy, well-maintained elder and periodontitis subjects.this investigation compared the site prevalence of 40 subgingival species in 30 periodontally healthy (mean age 36+/-9 years), 35 elders with a well-maintained periodontium (mean age 77+/-5) and 138 adult periodontitis subjects (mean age 46+/-11). subgingival plaque samples were taken from the mesial aspect of each tooth (up to 28 samples) in the 203 subjects at baseline. the presence and levels of 40 subgingival taxa were determined in 5003 plaque samples using whole genomic dna probes and chec ...19989650869
streptococcal bacteremia and colorectal cancer. 19989671848
antibacterial effects of a bioactive glass paste on oral microorganisms.bioactive glasses contain oxides of calcium, sodium, phosphorus, and silicon in a proportion that provides the material with surface activity and concomitantly with the property of forming a strong bond with bone. bioactive glasses have been tested as bone substitutes in different clinical situations. in an aqueous environment, ca2+, na+, po4(3-) , and si4+ are released from the glass, resulting in a rise in ph and in osmotic pressure in its vicinity. since these are factors that potentially inf ...19989688225
antimicrobial activity produced by six dentifrices.the antimicrobial activity of six commercially available dentifrices and positive and negative controls as they come into contact with streptococcus mutans, s. sanguis, and actinomyces viscosus commonly found in the oral cavity. sterile discs treated with the dentifrices were placed on agar plates with the controls. zones of microbial inhibition were measured in millimeters after 48 hours. all of the test dentifrices demonstrated antimicrobial activity against the bacteria.19989693543
detection of bacteraemia in patients with fever and neutropenia using 16s rrna gene amplification by polymerase chain reaction.episodes of fever and neutropenia are common complications of treatment for cancer. the use of prophylactic and early empirical antibiotics has reduced mortality but decreases the sensitivity of diagnostic tests based on culture. the aim of this study was to determine the potential of a broad diagnostic approach (eubacterial) based on 16s rrna gene amplification and sequencing to augment cultural methods of diagnosis of bacteraemia in patients with fever and neutropenia in a regional paediatric ...19989707307
in vitro studies of the effect of antiseptic-containing mouthwashes on the formation and viability of streptococcus sanguis biofilms.the aims of this study were to evaluate the growth of streptococcus sanguis on hydroxyapatite, bovine enamel and polytetrafluoroethylene substrata in a constant depth film fermentor, and to determine the effects of three antimicrobial-containing mouthwashes on biofilm formation and bacterial viability on hydroxyapatite and enamel. there was little difference in the final cell density (5 x 10(4) cfu mm-2) of the strep. sanguis biofilm on the three substrata. when hydroxyapatite-grown biofilms wer ...19989717301
antibacterial temporary filling materials: the effect of adding various ratios of ag-zn-zeolite.the effect of a new type of antibacterial temporary filling material was evaluated. ag-zn-zeolite (bactekiller, kanebo, japan) and sio2 filler were incorporated into urethane acrylate monomer paste in amounts of 5/55, 10/50, 20/40 and 30/30 wt%, respectively. the present study was designed to use a dye penetration test to measure direct inhibition of bacterial growth of four oral bacteria (streptococcus mutans, streptococcus mitis, streptococcus salivarius, streptococcus sanguis). the amounts of ...19989722093
dental plaque, platelets, and cardiovascular diseases.cardiovascular diseases, including atherosclerosis and myocardial ischemia, occur as a result of a complex set of genetic and environmental factors. during periodontitis, dental plaque microorganisms may disseminate through the blood to infect the vascular endothelium and contribute to the occurrence of atherosclerosis and risk of myocardial ischemia and infarction. myocardial ischemia and infarction are often preceded by acute thromboembolic events. in an in vitro model of thrombosis, certain d ...19989722699
influence of growth mode and sucrose on susceptibility of streptococcus sanguis to amine fluorides and amine fluoride-inorganic fluoride combinations.this study evaluated the susceptibility to amine fluorides (amfs) of planktonic and biofilm cultures of streptococcus sanguis grown with and without sucrose. cultures were incubated with amfs (250 mg of fluoride liter-1) for 1 min. the susceptibility of biofilms was less than that of the planktonic form and was further decreased by growth in the presence of sucrose.19989726905
composition of pellicles formed in vivo on tooth surfaces in different parts of the dentition, and in vitro on hydroxyapatite.saliva from the major salivary glands dominates different areas of the mouth. the parotid (ps) and submandibular/sublingual (sms) saliva differ in their protein composition, and thus, the composition of pellicles formed in various parts of the dentition might vary. in this study, proteins incorporated in 60-min pellicles from the premolar and front regions of the mouths of 4 subjects were examined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting using antibodie ...19989745119
[case from general practice. agranulocytosis]. 19989747134
oral microbiota and implant type membranes.candida albicans (ca), staphylococcus aureus (sa), streptococcus sanguis (ss), actinomyces naeslundii (an), actinomyces odontolyticus (ao), porphyromona spp (p spp), candida glabrata (cg), candida krusei (ck), and rhodotorula spp (r spp) were tested with equal pieces of biodegradable membranes. membranes pretreated with saliva or clorhexidine and nontreated control membranes were tested in three different culture media containing 0.1 ml homologous suspension for each strain under study. incubati ...19989759038
a streptococcal adhesion system for salivary pellicle and platelets.a streptococcus sanguis 133-79 adhesin identified by the monoclonal antibody 1.1 (mab 1.1) binds both saliva-coated hydroxylapatite (sha) and platelets. the complementary binding site(s) for the adhesin was identified by the anti-idiotypical mab 2.1. to learn if this adhesion system, marked by the antiadhesin mab 1.1 and anti-binding site mab 2.1, is commonly used by strains within the sanguis group and other viridans group streptococci, 42 strains from seven species were tested. strains that bi ...19989784548
proliferative response of cd8+ gamma delta+ t cells to streptococcus sanguis in patients with behçet's disease.to analyze the pathogenesis of behçet's disease (bd), we investigated the immune response of peripheral blood t cell subsets from patients with bd to streptococcus sanguis (s. sanguis) strain kth-i, a bacteria suggested to be associated with the etiology of bd. cultured peripheral blood mononuclear cells (pbmc) of patients with bd activated by lyophilized whole bacterium of s. sanguis were assayed by two-color flow cytometry using monoclonal antibodies (mabs) specific for gamma delta+ t cell rec ...19989785603
treated bacterial endocarditis as a histological mimic of fungal infection.histological assessment of cardiac valve tissue contributes to the diagnosis of infective endocarditis and is of particular importance in cases in which no organism is cultured. antibiotic treatment of bacterial endocarditis may lead to abnormal bacterial morphology and staining characteristics. although in many cases the presence of some residual bacteria of normal appearance makes the diagnosis straightforward, in some only abnormal bacteria may be seen. unless the appearances of these are int ...19989797734
acid-regulated proteins induced by streptococcus mutans and other oral bacteria during acid shock.our previous research has demonstrated that with the more aciduric oral bacteria, an acid shock to sub-lethal ph values results in the induction of an acid tolerance response that protects the cells at extremely low ph (ph 3.0-4.0) that kills unadapted control cells maintained at ph 7.5 (oral microbiol immunol 1997: 12: 266-273). in this study, we were interested in comparing the protein profiles of acid-shocked and control cells of nine organisms from three acid-ogenic genera that could be cate ...19989807121
response of single species biofilms and microcosm dental plaques to pulsing with chlorhexidine.the aim of this study was to determine the effect of pulsing chlorhexidine gluconate, at concentrations commonly used in mouthwashes, on streptococcus sanguis biofilms and microcosm dental plaques in vitro. biofilms were grown on bovine enamel and nutrients were supplied in the form of artificial saliva. pulsing experiments were carried out on steady-state biofilms using 0.05 or 0.2% chlorhexidine solutions delivered twice daily for 1 min. in a separate study, the enamel discs on which the biofi ...19989818743
bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite.the study aimed to evaluate intracanal irrigation procedures in eradicating bacteria from surface, shallow and deep layers of root dentine using extracted human teeth. artificial bacterial smear layer was successfully produced by rubbing a mixture of dental plaque and artificially decalcified dentine or carious dentine on root canal walls. the reservoir holes were 3.5 mm in depth, 1 mm in diameter prepared 1.5 mm apart and parallel to the root canals on the decrowned planes, in which five separa ...19989823113
streptococcus sanguis-induced platelet clotting in rabbits and hemodynamic and cardiopulmonary consequences.by mimicking hemostatic structural domains of collagen, streptococcus sanguis (aggregation-positive phenotype; agg+) induces platelets to aggregate in vitro. to test the hypothesis that aggregation occurs in vivo, s. sanguis (agg+ or agg- suspension) was infused intravenously into rabbits. the extent of hemodynamic and cardiopulmonary changes and the fate of circulating platelets were agg+ strain dose dependent. within 45 to 50 s of the start of infusion, 40 x 10(8) cfu of the agg+ strain caused ...19989826372
[alpha-hemolytic streptococci and root canal irrigants. an evaluation of the bactericidal efficacy of sodium hypochlorite and chlorhexidine gluconate plus cetrimide].the main bacterial species present in pulpal and periapical microbic flora is alpha-hemolytic streptococci. they are regarded as facultative anaerobes which prefer to grow in anaerobiosis. canal irrigation plays an important role in the success of endodontic treatment given that, on the one hand, it encourages the gradual elimination of the smear layer, and on the other it neutralises microbic flora in the root canal. the aim of this study was to test the microbiological efficacy of sodium hypoc ...19989835743
construction and analysis of a streptococcus parasanguis reca mutant: homologous recombination is not required for adhesion in an in vitro tooth surface model.pcr was used to amplify an internal region of the reca gene from streptococcus parasanguis fw213. the pcr fragment was used as a probe to recover the entire streptococcal reca gene from an s. parasanguis genomic library, and the sequence of the gene was determined. the deduced product of the s. parasanguis reca gene showed a high degree of amino acid identity with other prokaryotic reca proteins. the cloned reca sequence was disrupted in vitro by insertional mutagenesis, and the mutated allele w ...19999864313
[aortic valvular vegetation in annuloaortic ectasia due to idiopathic cystic medial necrosis: a case report].annuloaortic ectasia is often accompanied by marfan syndrome and associated with infective endocarditis usually involving the mitral valves. we treated a patient with annuloaortic ectasia due to idiopathic cystic medial necrosis who developed congestive heart failure with aortic valvular vegetation. a 56-year-old man had dyspnea on effort since the beginning of january, 1997 and was admitted to our hospital on april 6, 1997 because of orthopnea. the diagnosis was congestive heart failure due to ...19989864690
efficacy of trovafloxacin in treatment of experimental staphylococcal or streptococcal endocarditis.the efficacy of trovafloxacin against staphylococcus aureus and viridans group streptococci was investigated in vitro and in an experimental model of endocarditis. the mics at which trovafloxacin and ciprofloxacin inhibited 90% of clinical isolates of such bacteria (mic90s) were (i) 0.03 and 2 mg/liter, respectively, for 30 ciprofloxacin-susceptible s. aureus isolates, (ii) 32 and 128 mg/liter, respectively, for 20 ciprofloxacin-resistant s. aureus isolates, and (iii) 0.25 and 8 mg/liter, respec ...19999869569
purification and properties of a bacteriocin of staphylococcus epidermidis isolated from dental plaque.an extracellular bacteriocin of staphylococcus epidermidis isolated from dental plaque was purified and characterized. its molecular mass was 3500 da and pi was 10.5. this bacteriocin inhibited the growth of staphylococcus aureus, streptococcus salivarius, and streptococcus mitis, but streptococcus mutans, streptococcus sanguis and other oral indigenous bacterial species examined were not inhibited. the mode of inhibition was found to be bacteriostatic.19989872117
structures of biologically active muramyl peptides from peptidoglycan of streptococcus sanguis.the structures of major muramyl peptides derived from peptidoglycan of the oral pathogen streptococcus sanguis were determined and the biological activity of the peptides was tested in vitro on human monocytes. the muramyl peptides, produced by muramidase digestion of the purified peptidoglycan, were separated by reversed-phase high-performance liquid chromatography, either in their native form or after reduction with sodium borohydride. chemical structures of the peptides were elucidated by a c ...19989875522
bactericidal efficacy of carbon dioxide laser against bacteria-contaminated titanium implant and subsequent cellular adhesion to irradiated area.the aim of this study was to assess co2 laser ability to eliminate bacteria from titanium implant surfaces. the changes of the surface structure, the rise in temperature, and the damage of connective tissue cells after laser irradiation were also considered.19989888326
influence of different vehicles on the antibacterial effects of calcium hydroxide.this study evaluated the influence of three different vehicles on the antibacterial activity of calcium hydroxide against four bacterial species commonly found in endodontic infections. for this purpose, a broth dilution test using 24-well cell culture plates was performed. results showed that all pastes were effective in killing the bacteria tested, but at different times. the calcium hydroxide/camphorated paramonochlorophenol/glycerin paste was the most effective against the four bacterial str ...199810023249
turning on and turning off the arginine deiminase system in oral streptococci.the arginine deiminase system in oral streptococci is highly regulated. it requires induction and is repressed by catabolites such as glucose or by aeration. a comparative study of regulation of the system in streptococcus gordonii atcc 10558, streptococcus rattus fa-1, and streptococcus sanguis nctc 10904 showed an increase in activity of the system in s. sanguis of some 1467-fold associated with induction-depression of cells previously uninduced-repressed. the activity of the system was assaye ...199810030002
lipoteichoic acid acts as an antagonist and an agonist of lipopolysaccharide on human gingival fibroblasts and monocytes in a cd14-dependent manner.cd14 has been implicated as a receptor of lipoteichoic acid (lta) and other bacterial components as well as lipopolysaccharide (lps). since the structures of ltas from various gram-positive bacteria are heterogeneous, we analyzed the effects of ltas on the secretion of interleukin-8 (il-8) by high- and low-cd14-expressing (cd14(high) and cd14(low)) human gingival fibroblasts (hgf). while bacillus subtilis lta had an il-8-inducing effect on cd14(high) hgf which was considerably weaker than that o ...199910084995
pacemaker-related endocarditis. report of 7 cases and review of the literature.we report on 7 patients with pacemaker endocarditis diagnosed during the workup of long-standing fever. persistent positive blood cultures and echocardiography led to the diagnosis in 6 patients whereas autopsy was diagnostic in another. causative microorganisms were staphylococcus epidermidis (3), staphylococcus lugdunensis (1), pseudomonas aeruginosa (1), streptococcus bovis (1), and streptococcus mitis-streptococcus sanguis (1). pulmonary embolism was present in nearly 50% of the cases, a fig ...199810085483
effect of low-molecular-weight chitosans on the adhesive properties of oral streptococci.it was previously shown that a low-molecular-weight chitosan and its derivatives n-carboxymethyl chitosan and imidazolyl chitosan inhibit streptococcus mutans adsorption to hydroxyapatite. the ability of the same molecules to interfere with adhesive properties of other oral streptococci (streptococcus sanguis, streptococcus gordonii, streptococcus constellatus, streptococcus anginosus, streptococcus intermedius, streptococcus oralis, streptococcus salivarius, streptococcus vestibularis) was test ...199810093536
cumulative correlations of lysozyme, lactoferrin, peroxidase, s-iga, amylase, and total protein concentrations with adherence of oral viridans streptococci to microplates coated with human saliva.redundancy refers to the observation that many salivary proteins exhibit similar properties in vitro. it is possible that bacterial adherence to salivary pellicle occurs as a cumulative effect of multiple proteins. this study determined the joint and individual contributions of salivary amylase, s-iga, lysozyme, salivary peroxidase, lactoferrin, and total protein concentrations to adherence by oral viridans streptococci in microplates coated with whole saliva from 123 persons. strains used were: ...199910096451
[analysis of serum antibodies to alpha-streptococci in patients with tonsil-related pustulosis palmaris et plantaris].to determine the systemic immune response to alpha-streptococci (str. sanguis, str. salivarius and str. mitis) and beta streptococcus (str. pyogenes t12) in patients with tonsil related pustulosis palmaris et plantaris (ppp), we measured serum antibody levels to whole cell body antigens of streptococcus (str.) sanguis, str. salivarius, str. mitis or str. pyogenes by enzyme-linked immunosorbent assay (elisa). the serum igg antibody levels to alpha-streptococci, str. sanguis and str. salivarius we ...199910191624
cutaneous vasculitis and collapse. 199810197203
identification of oral mitis group streptococci by arbitrarily primed polymerase chain reaction."mitis group" streptococci are commensal but may play some role in dental caries, septicemia or endocarditis. rapid genotypic identification would aid studies of dental plaque ecology, or diagnostic use. ap-pcr with 58 unpaired arbitrary primers was used to characterize 7 streptococcus gordonii, 11 streptococcus sanguis, 2 streptococcus crista, 5 streptococcus parasanguis, 18 streptococcus oralis, and 36 streptococcus mitis (22 biovar 1 and 14 biovar 2). s. parasanguis 16s rrna variable region p ...199910204478
acid-induced acid tolerance and acidogenicity of non-mutans streptococci.acid tolerance and acidogenicity of non-mutans streptococci and their capacity of acid adaptation were studied. the cells of non-mutans streptococci (streptococcus sanguis [streptococcus sanguinis], streptococcus gordonii, streptococcus oralis and streptococcus mitis) grown at ph 7.0 showed 0.0088% to 71% viability after acidification at ph 4.0 for 60 min, whereas the cells of mutans streptococci (streptococcus mutans) were not killed by the acidification. washed cells of non-mutans streptococci ...199910204479
primary bacterial colonization of implant surfaces.the purpose of this study was to assess the effects of modifying titanium surfaces, in terms of wettability, roughness, and mode of sterilization, on the ability of the oral bacterium streptococcus sanguis to colonize. an in vitro model system was developed. all surfaces were colonized by the bacteria, but to significantly different levels. titanium samples that exhibited rough or hydrophobic (low wettability) surfaces, along with all autoclaved surfaces, were preferentially colonized (p < .01). ...199910212539
increased levels of transforming growth factor beta 1 and basic fibroblast growth factor in patients on capd: a study during non-infected steady state and peritonitis.long-term influence of continuous ambulatory peritoneal dialysis (capd) on concentrations of transforming growth factor beta1 (tgf-beta1) and basic fibroblast growth factor (bfgf) in the peritoneal effluent, and the effect of peritonitis on these cytokines were investigated. tgf-beta1 and bfgf were assayed in effluent samples from dialysate bags collected during the initial week of treatment with capd and at 5 months. to determine the effect of peritonitis, dialysate bags were collected on admis ...199910213269
an assessment of microbial coronal leakage in teeth root filled with gutta-percha and three different sealers.the aim of this in vitro study was to determine the effect of three different root canal sealers on the bacterial penetration of obturated root canals. eighty extracted human teeth with straight, single root canals were prepared using the modified double-flared technique with balanced force under copious irrigation until the master apical file was size 40. the teeth were divided randomly into experimental groups (60 teeth) and control groups (20 teeth). twenty experimental teeth each were obtura ...199610332239
effects of hydrogen peroxide on growth and selected properties of porphyromonas gingivalis.in this study we first evaluated the effects of hydrogen peroxide (h2o2) on growth and selected properties of porphyromonas gingivalis, and compared them with those obtained by a reducing agent (cysteine). the growth of p. gingivalis was only moderately affected when h2o2 was added at concentrations up to 30 mm in a complex culture medium. however, when a defined basal medium was used, h2o2 at a concentration of 3 mm completely inhibited growth of p. gingivalis. incorporation of cysteine at conc ...199910339828
infective endocarditis: an epidemiological review of 128 episodes.the objective was to determine the current epidemiology of infective endocarditis.199910342647
emergence of antibiotic resistant streptococcus sanguis in dental plaque of children after frequent antibiotic therapy.in the pediatric population, several different antibiotic regimens are currently recommended for the treatment of otitis media. this study investigated whether therapy for otitis media was associated with the emergence of antibiotic-resistant oral bacteria.199910355009
severe, rapidly progressing peri-implantitis.the replacement of teeth by means of titanium implants is a safe and predictable procedure in most patients. some studies show that a small number of patients lose the majority of their implants. unfortunately, it is impossible to identify these patients prior to surgery. it is important to report such cases so that we may, in the future, be able to identify them before surgery. the present case showed a severe and rapid exfoliation of titanium implants. out of 8 implants inserted in the anterio ...199910355623
the effects of histatin-derived basic antimicrobial peptides on oral biofilms.susceptibility of bacteria to antimicrobial agents is strongly reduced by the formation of complex biofilms. we investigated whether synthetic histatin analogs with broad-spectrum antibacterial activity in vitro were also active against these complex mixtures of bacteria, as present in saliva and plaque. in a simplified model system for dental plaque, hydroxyapatite discs were placed in a continuous culture system comprised of streptococcus mutans, s. sanguis, s. salivarius, actinomyces naeslund ...199910371248
removal of infected pacemaker leads with deep hypothermic circulatory arrest and open surgical exploration of the superior vena cava and innominate veins.despite the use of transvenous methods for extraction of infected leads, failed attempts may result in retained lead fragments. retained lead fragments may be a focus of continued infection leading to sepsis. we present two patients in which conversion from cardiopulmonary bypass to hypothermic circulatory arrest allowed direct visualization, using venotomies in the superior vena cava and innominate vein to achieve complete removal of retained pacemaker lead fragments. use of venotomies in the e ...199910392398
polymicrobial etiology of dental caries.the present study was carried out to establish the normal bacterial oral flora and the aerobic and anaerobic bacterial flora from deep seated dental caries, and to determine the antimicrobial sensitivity of the clinical isolates so obtained streptococcus mutans (48%) and streptococcus sanguis (20%) were the main aerobic isolates whereas lactobacillus spp. (52%), veillonella spp. (24%) and actinomyces spp. (12%) were the major anaerobic isolates. hundred percent of the samples from dental caries ...199910420681
cure of implantable venous port-associated bloodstream infections in pediatric hematology-oncology patients without catheter removal.the efficacy of antibiotic treatment of port-associated bloodstream infection without device removal has not been systematically studied. we analyzed the outcome of 43 consecutive port-associated bloodstream infections in pediatric hematology-oncology patients. etiologies included staphylococcus epidermidis (30) and staphylococcus aureus (6). antibiotics were given through the port for a median of 11 days. four ports were removed within 72 hours. in 36 (92%) of the remaining 39 episodes, there w ...199910433571
deposition and retention of vital and dead streptococcus sanguinis cells on glass surfaces in a flow-chamber system.the proportion of vital as compared with dead streptococcus sanguinis cells attached to glass surfaces was monitored and related to varying proportions of planktonic vital as compared with dead strep. sanguinis cells. in a flow chamber with six parallel-mounted glass plates, strep. sanguinis was suspended in pretreated sterile human saliva. deposition of strep. sanguinis took place, with a proportion of vital sanguinis streptococci in saliva (%vss) of 90%, 45% or 22.5%. after exposure times of 3 ...199910459773
in vitro activities of fluoroquinolones against antibiotic-resistant blood culture isolates of viridans group streptococci from across canada.among 418 blood culture isolates of viridans group streptococci obtained between 1995 and 1997, the in vitro rates of nonsusceptibility to penicillin, erythromycin, tetracycline, and trimethoprim-sulfamethoxazole were 28, 29, 24, and 14%, respectively. the most prevalent group (125 strains) was streptococcus mitis, followed by streptococcus sanguis (56 strains). for 236 (56%) strains resistant to one or more antibiotics, the ciprofloxacin mic at which 90% of the isolates were inhibited (mic(90)) ...199910471583
adhesion and surface-aggregation of candida albicans from saliva on acrylic surfaces with adhering bacteria as studied in a parallel plate flow chamber.adhesive interactions between candida albicans and oral bacteria are generally thought to play a crucial role in the microbial colonization of denture acrylic, which may lead to denture stomatitis. this study investigated the influence of saliva on the adhesive interactions between c. albicans and streptococcus sanguis or actinomyces naeslundii on denture acrylic. first, bacteria were allowed to adhere to the acrylic surface from a flowing suspension, and subsequently yeasts were flowed over the ...199910510723
genetic approaches to the identification of the mitis group within the genus streptococcus.the usefulness and reliability of partial sequence analysis of the manganese-dependent superoxide dismutase gene (soda), autolysin (lyta) gene amplification and species-specific pcr based on the d-alanine:d-alanine ligase (ddl) gene for differentiating each member of the mitis group of the genus streptococcus was investigated. on the phylogenetic tree based on soda partial sequences (366 bp) from 96 strains, including all species currently within the mitis group isolated in different geographic ...199910517614
an in vitro investigation of the antimicrobial activity of an herbal mouthrinse.the purpose of this study was to determine the antimicrobial activity of one prescription and two commercially available over-the-counter mouthrinses and positive and negative controls against bacteria commonly found in the oral cavity. the bacteria used in this study were streptococcus mutans, streptococcus sanguis and actinomyces viscosus. sterile discs which were treated with the mouthrinses were placed on agar plates along with the controls. zones of microbial inhibition were measured in mil ...199810518853
automatic enumeration of adherent streptococci or actinomyces on dental alloy by fluorescence image analysis.the aim of the present study was to develop an automated image analysis method to quantify adherence of streptococcus sanguinis or actinomyces viscosus on surfaces of a currently used dental alloy. counting such bacterial strains was difficult because of their arrangement, thus s. sanguinis being a coccus arranged in chains or pairs, and a. viscosus a long complexly arranged polymorph rod. direct counting of fluorescently stained adherent bacteria was done visually and with image analysis method ...199910520583
inhibitory effects of oolong tea extract on caries-inducing properties of mutans streptococci.the inhibitory effects of oolong tea extract (ote) on the caries-inducing properties of mutans streptococci were examined in vitro. ote reduced the rate of acid production by mutans streptococci accompanied with the retardation of growth rate of mutans streptococci, while the action by chromatographically isolated oolong tea polyphenol (otf6) was weak. on the other hand, both oolong tea products decreased cell surface hydrophobicity of almost all the oral streptococci examined in the present stu ...199910529529
beta-hemolytic streptococci and other beta-hemolytic organisms in apical periodontitis and severe marginal periodontitis.thirteen teeth with necrotic pulps and apical periodontitis and nine severe periodontal pockets were cultured for presence of beta-hemolytic streptococci and other beta-hemolytic organisms. samples were dispersed and plated on two non-selective and one selective growth media and incubated anaerobically and in 10% co2 in air. a total of 59 beta-hemolytic colonies were purified and identified. eight beta-hemolytic streptococcal isolates were obtained from three of the severe marginal periodontitis ...199910530151
an evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria.the aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: facultative aerobes-anaerobes: candida albicans atcc 10231; enterococcus faecalis atcc 29212; escherichia coli atcc 25,922; pseudomonas aeruginosa atcc 27,853; streptococcus mitis atcc 9811; streptococcus mutans atcc 35668; streptococcus salivarius atcc 13419; and streptococcus sanguis atcc 10556. microaerophiles: actinobacillus actinom ...199910530260
bacterial species- and strain-dependent induction of tissue factor in human vascular endothelial cells.a cardinal process in bacterial endocarditis (be) is the activation of the clotting system and the formation of a fibrin clot on the inner surface of the heart, the so-called endocardial vegetation. the processes that lead to the activation of the clotting system on endothelial surfaces upon exposure to bacteria are largely unknown. in the present study, we investigated in an in vitro model whether infection of human endothelial cells (ec) with bacteria that are relevant to be, such as staphyloc ...199910531276
multiple infections in carotid atherosclerotic plaques.chlamydia pneumoniae, cytomegalovirus, herpes simplex virus, and recently, periodontal disease, have been associated with human atherosclerosis. porphyromonas gingivalis and streptococcus sanguis are major pathogens associated with periodontitis, a common chronic inflammatory condition in adults. investigators have found that these infectious agents may influence vascular cell functions by inducing thrombus formation, vascular cell proliferation, apoptosis, and cell death.199910539867
efficacy of levofloxacin for experimental aortic-valve endocarditis in rabbits infected with viridans group streptococcus or staphylococcus aureus.levofloxacin is among the more active fluoroquinolones against streptococci and staphylococci. it is effective against moderately severe infections caused by these organisms, but its efficacy in the treatment of bacteremia and serious infections such as endocarditis is not well defined. we compared the efficacy of levofloxacin to those of standard agents in the rabbit model of aortic-valve endocarditis caused by fluoroquinolone-susceptible strains including a penicillin-susceptible strain of str ...199910543757
membrane locus and ph sensitivity of paraben inhibition of alkali production by oral streptococci.parabens were found to be potent inhibitors of alkali production from arginine by oral streptococci such as streptococcus rattus, streptococcus sanguis and streptococcus gordonii. for example, 2 mumol butylparaben per ml completely and irreversibly inhibited arginolysis by intact cells of s. rattus fa-1 and was lethal for the organism. in contrast, butylparaben was not a very effective inhibitor of ureolysis by intact cells of streptococcus salivarius 57.i, although it did kill the cells. butylp ...199910551169
identification of clinically relevant viridans streptococci by analysis of transfer dna intergenic spacer length polymorphism.the utility of pcr analysis of transfer dna intergenic spacer length polymorphism (tdna-ilp) for the identification to the species level of clinically relevant viridans streptococci was evaluated with a collection of reference strains of 15 species of the salivarius, anginosus, mitis and mutans rrna homology groups. pcr products generated by using fluorescent, outwardly directed, consensus tdna primers were analysed by electrophoresis on denaturating polyacrylamide gels and by laser fluorescence ...199910555340
reversed-phase high-performance liquid chromatographic measurement of the p-aminobenzoic acid synthesized by streptococcus sanguis.this study investigated the biosynthesis of p-aminobenzoic acid by streptococcus sanguis and the role of p-aminobenzoic acid in the interaction between streptococcus sanguis and streptococcus mutans.199810557192
prevalence of the amylase-binding protein a gene (abpa) in oral streptococci.salivary amylase binds specifically to a number of oral streptococcal species. this interaction may play an important role in dental plaque formation. recently, a 585-bp gene was cloned and sequenced from streptococcus gordonii challis encoding a 20.5-kda amylase-binding protein (abpa). the goal of this study was to determine if related genes are present in other species of oral streptococci. biotinylated abpa was used in southern blot analysis to screen genomic dna from several strains represen ...199910565935
experimental uveitis induced by intravitreal or intravenous lipoteichoic acid in rabbits.to investigate the role of lipoteichoic acid (lta), one of the cell wall components in gram-positive bacteria in uveitis.199910580658
the transfer of bacteria to, and survival on, dental records.the purpose of this study was to ascertain whether viable bacteria could be transferred from gloves to laboratory cards in dental records and, if so, to determine whether bacteria could survive on the paper. the thumbs and forefingers of two types of glove (biogel d and microtouch) were inoculated with streptococcus sanguis and left for various periods of time. a sterile dental laboratory card was then held with the gloves and the number of bacteria surviving on the card determined after various ...199910581730
investigation of bacteremia after orthodontic banding.the aim of this study was to assess the incidence of bacteremia after orthodontic banding. the study was conducted on 40 healthy orthodontic patients with good oral hygiene. venous blood samples were obtained with a strict aseptic technique before and after fitting of a molar band in each patient. microbiologic evaluation of the samples revealed a postoperative bacteremia incidence of 7.5%.199910587604
efficacy of levofloxacin in the treatment of experimental endocarditis caused by viridans group streptococci.levofloxacin was investigated against viridans group streptococci in vitro and in rats with experimental aortic endocarditis. the mic(90)s of levofloxacin and ciprofloxacin for 20 independent isolates of such bacteria were 1 and 8 mg/l, respectively. rats were infected with two types of organism: either fully susceptible to levofloxacin mic < or = 0.5 mg/l) or borderline susceptible (mic 1-2 mg/l). fully levofloxacin-susceptible bacteria comprised one penicillin-susceptible (mic 0.004 mg/l) stre ...199910590278
systemic doxycycline administration in the treatment of periodontal infections (ii). effect on antibiotic resistance of subgingival species.the purpose of this investigation was to determine the proportion and prevalence of doxycycline resistant species in subgingival plaque samples taken during and after doxycycline administration. 20 subjects with adult periodontitis were randomly assigned to test (n = 10) or control groups (n = 10). saliva samples as well as subgingival plaque samples taken from the distal surface of 6 posterior teeth were collected at baseline. all subjects received full mouth srp and the test group systemic dox ...199910599905
comparative in vitro activity of moxifloxacin against gram-positive clinical isolates.the in vitro activity of moxifloxacin was compared with that of 15 antibacterial agents against 513 gram-positive microorganisms. the mic(90) (mg/l) of moxifloxacin was 0.06 for quinolone-susceptible staphylococcus aureus and staphylococcus epidermidis, 0.12 for streptococcus pyogenes and streptococcus agalactiae; 0.25 for streptococcus pneumoniae, streptococcus mitis, streptococcus bovis, streptococcus anginosus and actinomyces pyogenes; 0.5 for streptococcus sanguis and listeria monocytogenes, ...200010629010
periodontal findings and systemic antibody responses to oral microorganisms in behçet's disease.behçet's disease is a multisystem disorder of unknown etiology, affecting predominantly the oral mucosa, skin, and eyes. recurrent and painful episodes of oral ulcerations interfere with regular oral hygiene leading to rapid bacterial plaque accumulation. the aims of this study were to evaluate the periodontal status of patients with behçet's disease and determine serum antibody responses to selected oral microorganisms, including major periodontopathogens in these patients.199910632520
adhesion of candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface.adhesion of candida albicans to saliva-coated surfaces is an important early step in the colonization of the oral cavity. c. albicans cells also adhere to several species of oral streptococci including streptococcus gordonii, streptococcus oralis and streptococcus sanguinis in what are believed to be multi-modal interactions. it is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of c. albicans cells by up to 2-3-fol ...200010658650
[effects of surface roughness of two restorative materials on early streptococcus sanguis adhesion in vitro].this was an in vitro study aimed at the surface roughness of two kinds of commonly used restorative materials resin and alloy necessary to affect the adhesive behavior of streptococcus sanguis(s. s) on them after 24 h. the surface roughness(sr) of each tested sample was evaluated with a profilometer quantitatively and observed with scanning electron microscope (sem) morphologically. then the adhesive microbial amount was determined by the clone forming unit counting method, and adhesion morpholo ...199810684003
synthesis, microbicidal activity, and solution structure of the dodecapeptide from bovine neutrophils.the dodecapepetide sequence r-l-c-r-i-v-v-i-r-v-c-r with a disulfide bridge between the cysteine residues found in bovine neutrophils was synthesized by solid-phase procedures. its antimicrobial activity against oral microorganisms such as actinobacillus actinomycetemcomitans, porphyromonas gingivalis, streptococcus mutans, and streptococcus gordonii was examined, and its structural features were examined by cd and determined by two-dimensional (2d) nmr. the strains p. gingivalis (w50 and 381), ...200010685049
factors which are associated with dental decay in the older individual.to improve reliability of salivary bacterial cultures as a surrogate for plaque levels of cariogenic bacterial species by reporting the salivary cfus of these organisms as a function of the number of teeth.199910687507
bacterial microleakage of cavit, irm, term, and fermit: a 21-day in vitro study.the aim of our study was to evaluate the leakage of four cements (cavit, irm, term, and fermit) using a two-compartment model system and streptococcus sanguis as bacterial marker. access cavities in premolars were filled with cement and the teeth immersed in culture medium in the model system. half of the teeth were thermocycled on day 2. bacterial percolation into the upper compartment was measured at regular intervals (days 2, 7, 14, and 21). cement thickness was measured at the end of the stu ...199910687522
effects of organic acid anions on growth, glycolysis, and intracellular ph of oral streptococci.oral streptococci produce large quantities of organic acids as the end-products of carbohydrate fermentation. in an approach to determine if oral streptococci exhibit differential sensitivities to organic acid anions, we determined the effects of formate, lactate, and acetate on intracellular ph maintenance, glycolysis, and growth of streptococcus mutans and streptococcus sanguis. growth was determined as maximum culture optical density in the presence of the organic acid anions at ph 7.1, 6.7, ...200010690666
co-aggregation as a virulent factor of streptococcus sanguis isolated from infective endocarditis.the pathogenicity of strains of the streptococcus sanguis group, isolated from infective endcarditis, was studied by measuring the development of subcutaneous abscesses in mice after infection with s. sanguis and actinomyces viscosus either singly or as co-aggregated pairs. the pathogenicity of the co-aggregates was also examined in various viable combinations of the two bacterial species. more abscesses were formed by a. viscosus than the s. sanguis group including clinical isolates. abscess fo ...199910692836
in vitro antimicrobial activity of propolis and arnica montana against oral pathogens.arnica and propolis have been used for thousands of years in folk medicine for several purposes. they possess several biological activities such as anti-inflammatory, antifungal, antiviral and tissue regenerative, among others. although the antibacterial activity of propolis has already been demonstrated, very few studies have been done on bacteria of clinical relevance in dentistry. also, the antimicrobial activity of arnica has not been extensively investigated. therefore the aim here was to e ...200010716618
[lumbosacral spondylodiscitis as the first manifestation of subacute bacterial endocarditis caused by streptococcus sanguis type ii]. 200010721582
salivary film expresses a complex, macromolecular binding site for streptococcus sanguis.teeth in the oral cavity are coated with a salivary film or pellicle, which lacks apparent intermolecular organization. this heterogeneous film facilitates binding of early commensal colonizing bacteria, including streptococcus sanguis. to test the hypothesis that sufficient intermolecular organization exists in salivary films to form binding sites for s. sanguis, an in vitro model of saliva-coated teeth was probed with murine anti-idiotypical monoclonal antibodies (mab2, anti-ids). the anti-ids ...200010722745
namibian chewing stick, diospyros lycioides, contains antibacterial compounds against oral pathogens.the twigs of diospyros lycioides, a plant commonly known as "muthala", are frequently used as chewing sticks for the cleaning of teeth by rural and urban people in namibia. preliminary studies showed that a methanol extract of d. lycioides inhibited growth of selected oral pathogens. subsequent bioassay-guided fractionation led to the isolation of four novel bioactive naphthalene glycosides, diospyrosides a, b, c, and d (1-4), and two known bioactive naphthoquinones, juglone (5) and 7-methyljugl ...200010725172
investigation of bacteremia following orthodontic debanding.the aim of this study was to investigate the prevalence of bacteremia after orthodontic debanding and debonding. the study group comprised 30 patients (10 men, 20 women). all were treated using the edgewise technique. patients with acceptable oral hygiene and fixed appliances in both jaws were included in the study group. blood samples were obtained using a strict aseptic technique before and after removal of bands and brackets. a 6.6% bacteremia prevalence was observed in both preoperative and ...200010730670
purification, characterization, and molecular analysis of the gene encoding glucosyltransferase from streptococcus oralis.streptococcus oralis is a member of the oral streptococcal family and an early-colonizing microorganism in the oral cavity of humans. s. oralis is known to produce glucosyltransferase (gtase), which synthesizes glucans from sucrose. the enzyme was purified chromatographically from a culture supernatant of s. oralis atcc 10557. the purified enzyme, gtase-r, had a molecular mass of 173 kda and a pi of 6.3. this enzyme mainly synthesized water-soluble glucans with no primer dependency. the addition ...200010768934
profile of subgingival microbiota in children with primary dentition.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ...200010791707
comparison of gyra and parc mutations and resistance levels among fluoroquinolone-resistant isolates and laboratory-derived mutants of oral streptococci.laboratory-derived fluoroquinolone-resistant mutants were obtained by serial passage of streptococcus sanguis and streptococcus anginosus isolates on agar containing increasing concentrations of old and new fluoroquinolones, ofloxacin and du-6859a, respectively. sequencing of an s. sanguis isolate exposed to du-6859a showed that resistance was associated with two mutations in the quinolone resistance determining region (qrdr) of the gyra gene (ser83-->phe; glu87-->lys), and with a mutation in th ...200010837428
effect of an essential oil-containing antiseptic mouthrinse on induction of platelet aggregation by oral bacteria in vitro.with an increasing body of data suggesting an association between periodontitis and cardiovascular disease, studies have been conducted to elucidate potential mechanisms by which oral bacteria might exert systemic effects. 2 oral bacteria, streptococcus sanguis and porphyromonas gingivalis, have been shown to induce platelet aggregation in vitro. this study was conducted to determine the effect of treatment with an essential oil mouthrinse (listerine antiseptic) on the platelet-aggregating activ ...200010847543
differential patterns of apoptosis in resolving and nonresolving bacterial pneumonia.infection with either streptococcus sanguis or streptococcus pneumoniae type 25 causes acute pneumonitis in rats. pneumonia caused by s. sanguis resolves over the course of 8 d, whereas pneumonia caused by s. pneumoniae type 25 progresses to fibrosis. to examine the role of apoptosis in these models, we performed assays with the terminal deoxynucleotidyltransferase-uridine nucleotide end-labeling technique on tissue sections from rat lungs at various times, and quantified the results with image ...200010852786
natural history of streptococcus sanguinis in the oral cavity of infants: evidence for a discrete window of infectivity.the heterogeneous group of oral bacteria within the sanguinis (sanguis) streptococci comprise members of the indigenous biota of the human oral cavity. while the association of streptococcus sanguinis with bacterial endocarditis is well described in the literature, s. sanguinis is thought to play a benign, if not a beneficial, role in the oral cavity. little is known, however, about the natural history of s. sanguinis and its specific relationship with other oral bacteria. as part of a longitudi ...200010858217
ib-367, a protegrin peptide with in vitro and in vivo activities against the microflora associated with oral mucositis.although the microflora associated with oral mucositis initiated by cytotoxic therapy is not well characterized, several studies suggest that reduction of the microbial load in the oral cavity has some clinical benefit. the mics of ib-367, a synthetic protegrin analog, ranged from 0.13 to 64 microgram/ml for gram-positive bacteria (streptococcus mitis, streptococcus sanguis, streptococcus salivarius, and staphylococcus aureus) and from 0.06 to 8 microgram/ml for gram-negative species (klebsiella ...200010858334
studies concerning the glucosyltransferase of streptococcus sanguis.we have shown in previous studies that the glucosyltransferase (gtf) enzymes of streptococcus mutans have distinct properties when adsorbed to a surface. in the present study, we compared the activity of gtf from streptococcus sanguis, designated gtfss, in solution and on the surface of saliva-coated hydroxyapatite (sha) beads, and determined the ability of its product glucan to support the adherence of oral microorganisms. gtf from s. sanguis 804 nctc 10904 was purified from culture supernatant ...200010867431
[meningitis after spinal anesthesia].the occurrence of meningitis after spinal anaesthesia is a very rare event. we report a case of streptococcus sanguis meningitis following spinal anaesthesia for orthopaedic material removal. the presence of gram positive cocci (streptococcus sanguis) in the cerebrospinal fluid was in favour of an exogenous contamination, originating either from the patient's skin or the anaesthesiologist's oropharynx. the outcome was uneventful. the responsibility of the latter can result in legal consequences. ...200010874434
role of monocytes in experimental staphylococcus aureus endocarditis.in the pathogenesis of bacterial endocarditis (be), the clotting system plays a cardinal role in the formation and maintenance of the endocardial vegetations. the extrinsic pathway is involved in the activation of the coagulation pathway with tissue factor (tf) as the key protein. staphylococcus aureus is a frequently isolated bacterium from patients with be. we therefore investigated whether s. aureus can induce tf activity (tfa) on fibrin-adherent monocytes, used as an in vitro model of be. we ...200010899897
colorimetric indicators of microbial contamination in corneal preservation medium.to compare acid-base and oxidation-reduction indicators and to investigate the effect of buffer and temperature on the colorimetric detection of microbial growth in corneal preservation media.200010928770
effect of the interleukin-1 genotype on monocyte il-1beta expression in subjects with adult periodontitis.an association has been reported between polymorphisms in the genes encoding il-1alpha (-889) and il-1beta (+3953) (periodontitis susceptibility trait, pst), and an increased severity of periodontitis (18). the il-1beta polymorphism was reported to correlate with increased il-1beta expression by monocytes in response to bacterial stimulants. in the present study, we determined if pst positive subjects with periodontitis exhibit elevated production of il-1beta, compared to pst negative periodonti ...200010929872
taxonomic study of "tufted mitior" strains of streptococci (streptococcus sanguinis biotype 11); recognition of a new genospecies.the taxonomic position of tufted strains of streptococci, phenotypically resembling streptococcus mitis and previously referred to as 'tufted mitior' was investigated. by 16s rrna sequence analysis, it was clear that the "tufted mitior" strains belonged to the mitis group of species within the genus streptococcus. it was confirmed that these strains were taxonomically independent at the species level, sharing less than 43%, dna-dna similarity with all established species of the mitis group. howe ...200010930077
trovafloxacin treatment of viridans group streptococcus experimental endocarditis.the activity of trovafloxacin was compared with those of vancomycin and penicillin in a model of streptococcus sanguis species group (trovafloxacin mic, 0.125 microg/ml) and streptococcus mitis species group (trovafloxacin mic, 0.125 microg/ml) experimental endocarditis. rabbits with catheter-induced aortic valve vegetations were given no treatment, trovafloxacin at 15 mg/kg of body weight three times a day (t.i.d.), vancomycin at 15 mg/kg twice a day, or penicillin at 1. 2 x 10(6) iu t.i.d. aft ...200010952616
antibody-targeted lethal photosensitization of porphyromonas gingivalis.we have previously demonstrated that porphyromonas gingivalis is susceptible to killing by toluidine blue o (tbo) when irradiated with light from a helium-neon (hene) laser. the aim of this study was to determine whether a tbo-antibody conjugate (ab-tbo) could be used to specifically target p. gingivalis to lethal photosensitization in the presence of streptococcus sanguis or human gingival fibroblasts (hgfs). when a mixture of p. gingivalis and s. sanguis was exposed to 4 microg of tbo/ml and i ...200010991833
presence of bacterial 16s ribosomal rna gene segments in human intestinal lymph follicles.there is currently no information regarding microbial agents inside the intestinal lymph follicles.200010994621
effects of antibodies to glucosyltransferase on soluble and insolubilized enzymes.previous studies have shown that glucosyltransferase enzymes (gtfs) of streptococcus mutans adsorbed to saliva coated hydroxyapatite (sha) have distinct properties from the same enzymes in solution. the purpose of the present study was to determine the effects on enzyme activity of polyclonal antibodies raised to gtfs in a soluble form and bound to sha.200011002411
effects of apis mellifera propolis on the activities of streptococcal glucosyltransferases in solution and adsorbed onto saliva-coated hydroxyapatite.propolis, a resinous hive product collected by apis mellifera bees, has been used for thousands of years in folk medicine. ethanolic extracts of propolis (eep) have been shown to inhibit the activity of a mixture of crude glucosyltransferase (gtf) enzymes in solution. these enzymes synthesize glucans from sucrose, which are important for the formation of pathogenic dental plaque. in the present study, the effects of propolis from two different regions of brazil on the activity of separate, purif ...200011014909
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