Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [immunity to diphtheria in advanced cancer patients]. | in expectation of a possible anticancer treatment with diphtheria toxin, the antidiphtheria immunity has been tested on 65 advanced cancerous patients. the test has been carried out by schick's test and by blood antibodies' dosage effected by a process "in vivo" on the depilated skin of the new zealand rabbit. the results show that about the 30% of these patients has no antidiphtheria immunity. the remaining 70% has antidiphtheria immunity, even if in half cases the antibody level is lower than ... | 1980 | 6783052 |
| esculin hydrolysis by gram positive bacteria. a rapid test and it's comparison with other methods. | a number of bacteria hydrolyze esculin enzymatically to esculetin. this characteristic is used by taxonomists and clinical microbiologists in the differentiation and identification of bacteria, especially to distinguish lance-field group d streptococci from non-group d organisms and listeria monocytogenes from morphologically similar erysipelothrix rhusipoathiae and diphtheroids. conventional methods used for esculin hydrolysis require 4--48 h for completion. we developed and evaluated a medium ... | 1981 | 6783825 |
| counter immunoelectrophoresis for rapid testing of toxin producing corynebacterium diphtheriae. | 1980 | 6785212 | |
| immunization status of school children. | 1980 | 6785226 | |
| regulation of toxinogenesis in corynebacterium diphtheriae. i. mutations in bacteriophage beta that alter the effects of iron on toxin production. | diphtherial toxin is produced in maximal yields by corynebacterium diphtheriae (c7(beta tox+) only when iron is present in growth-limiting amounts. toxin production is markedly decreased under high-iron conditions. we studied the role of the bacteriophage beta genome in this apparent regulation of toxin production by iron. using a passive immune hemolysis assay to detect toxin antigen production in individual plaques, we identified rare phage mutants that were toxinogenic in high-iron medium. ly ... | 1981 | 6785449 |
| regulation of toxinogenesis in corynebacterium diphtheriae. ii. genetic mapping of a tox regulatory mutation in bacteriophage beta. | the structural gene for diphtherial toxin is present in corynebacteriophage beta. previous studies located several point mutations within the tox locus and determined the orientation of transcription of the toxin structural gene. the production of maximal quantities of toxin by corynebacterium diphtheriae c7(beta) occurs only when the bacteria are iron deficient. mutations in phage beta can affect this control of toxin production by iron. the tox-201 mutation regulates expression of the toxin st ... | 1981 | 6785450 |
| diphtheroid endocarditis after electrolysis. | 1981 | 6786539 | |
| [varieties of diphtheria: microbiological diagnosis and epidemiological observations (author's transl)]. | following several cases of diphtheria an epidemiological investigation was made in and around aachen. a total of 1458 throat and nose smears were examined for various corynebacteria species. other than in two patients who had diphtheria, toxin-producing corynebacterium diphtheriae was demonstrated in only one person who had been in immediate contact with them. of a total of 150 corynebacteria of different species which were isolated, two further isolates proved to be non-toxin-producing diphther ... | 1981 | 6786851 |
| corynebacterium diphtheriae in surgical wounds. | 1981 | 6786985 | |
| [antigenic characteristics of various toxigenic and non-toxigenic strains of c. diphtheriae var. gravis]. | 1981 | 6787823 | |
| an improved method for obtaining homogeneous and stable suspension of c. diphtheriae for bacterial agglutination. | 1980 | 6788014 | |
| slide agglutination assay in the c. diphtheriae identification. | 1980 | 6788015 | |
| chromogenic detection of antigen in bacteriophage plaques: a microplaque method applicable to large-scale screening. | we have developed a simple and rapid (24 h) enzyme-linked immuno-detection method to screen for rare antigen-positive phage among large numbers of antigen-negative ones. horse-radish peroxidase-antibody conjugate, incorporated into the soft agar layer of a plaque assay system, is precipitated locally by antigen produced during plaque formation, and is detected by standard chromogenic methods. the method has been used to screen plaques of bacteriophage beta tox+ for the presence of diphtheria tox ... | 1981 | 6790347 |
| appendix: a model of plaque formation. | equations describing plaque formation in soft agar have been based on certain simplifying assumptions, for which data are presented. the derived equations permit one to calculate (i) average plaque size as a function of the initial density of indicator cells (do), (ii) the number of cells lysed per plaque as a function of do, and (iii) the cumulative number of cells lysed at various stages of plaque development. the calculated values agree well with those determined experimentally. | 1981 | 6790348 |
| [diphtheria and tetanus antibodies in children and young adults (author's transl)]. | diphtheria and tetanus antibodies were determined in a random sample of 635 test persons of 1 to 25 years of age via the passive haemagglutination test. using sera and immunoglobulins standardized for protective capacity, titres were expressed as international units (iu). titres greater than or equal to 0.01 iu/ml diphtheria antitoxin were detected in 96% of children (1-17 years, n = 70), in 79% of adults aged 18-22 (n = 397), in 68% of adults aged 23-25 (n = 168); tetanus antitoxin was found in ... | 1981 | 6791906 |
| [microflora of the gastric and duodenal mucosa in peptic ulcer]. | 1981 | 6792417 | |
| [epidemiological surveillance in diphtheria infections]. | 1981 | 6797161 | |
| mutants of corynebacterium diphtheriae pw8 that produce toxin in medium with excess iron. | two mutants that produce toxin in medium with excess iron were isolated from the pw8 strain of corynebacterium diphtheriae. these mutants produced as much toxin in medium containing excess iron (3 mug of fe2+ per ml) as did the parent pw8 strain in iron-depleted medium, and they will be very useful for easy production of toxin. | 1981 | 6797351 |
| diphtheria toxin and related proteins: effect of route of injection on toxicity and the determination of cytotoxicity for various cultured cells. | the effect of route of injection on the toxicity of intact diphtheria toxin, cross-reacting material (crm45), and diphtherial fragment a was compared in several animal species. by ordinary routes of injection, neither crm45 nor fragment a was toxic, even in species for which 0.1 micrograms of toxin/kg of body weight was lethal. after intracerebral injection, however, small amounts of crm45 led to paralysis and death, even in mice and rats--species that are resistant to toxin administered intrave ... | 1982 | 6798133 |
| the exotoxins of corynebacterium ulcerans. | the exotoxins produced by ten strains of c. ulcerans (two human, six bovine and two equine) have been studied. on the criteria of toxin-antitoxin neutralisation and immunoprecipitation tests using highly specific diphtheria and c. ovis antitoxins with crude toxic filtrates, (nh4)2so4 concentrates, and partially purified chromatographic preparations of these, together with the presence or absence of inhibition of the action of staphylococcal beta-haemolysin, and the reaction produced when injecte ... | 1982 | 6801113 |
| [accelerated identification method for corynebacterium diphtheriae]. | a method for rapid isolation of c. diphtheriae and determination of its biovar is proposed. the method consists in the inoculation of the material under study into 5% blood agar, the determination of cystinase in the modified pisou medium prepared from agar d with sodium hyposulfite added and the fermentation of starch in dishes with serum starch agar. the proposed method allows one to reduce the time necessary for the identification of c. diphtheriae by 18 hours. | 1982 | 6805177 |
| preliminary crystallographic investigation of the protein toxin from corynebacterium diphtheriae. | crystals of diphtheria toxin have been obtained in vapor diffusion experiments from concentrated solutions of potassium tartrate as the precipitating agent. the crystals are trigonal with unit cell dimensions a = b = 97.90 a and c = 100.30 a. the space group is p31 12 or its enantiomorph. the unit cell contains 6 molecules of molecular weight 62,000 with 1 molecule/asymmetric unit. the crystals diffract beyond 2.8 a resolution and are able to withstand more than 100 h of x-ray exposure. the comp ... | 1982 | 6806258 |
| burns and corynebacterium diphtheriae. | 1982 | 6807498 | |
| production of corynebacterium diphtheriae toxin in a bubble column fermentor. | 1982 | 6807989 | |
| [evaluation of the fluorescence for the identification of corynebacterium diphtheriae in patients with diphtheria]. | 1981 | 6808630 | |
| tetracycline resistance of corynebacterium diphtheriae isolated from diphtheria patients in jakarta, indonesia. | of 133 corynebacterium diphtheriae isolates from diphtheria patients in jakarta, indonesia, 86% were resistant to greater than or equal to 32 micrograms of tetracycline per ml. all isolates were sensitive to ampicillin, cephalothin, chloramphenicol, clindamycin, penicillin, erythromycin, and kanamycin. the general resistance of c. diphtheriae to tetracycline in this part of indonesia appears to be unique compared with resistance reported in studies done in other parts of the world. | 1982 | 6808914 |
| [bacterial endocarditis due to corynebacterium diphtheriae (author's transl)]. | a case of bacterial endocarditis with unusual features in a 5 year-old boy is reported. there was no underlying heart disease or provocating factor. the organism was a non toxigenic corynebacterium diphtheriae which was difficult to classify. it appeared to be close to the jk group of corynebacterium described by the center for disease control and considered as possibly responsible for severe infections. however, it was sensitive to penicillin. the course of the disease was complicated by the de ... | 1982 | 6808967 |
| group jk diphteroids sepsis in a leukaemic patient. | 1982 | 6812335 | |
| diphtheria: studies on the biology of an infectious disease. | 1980 | 6813290 | |
| the lowly diphtheriod: nondiphtheria corynebacterial infections in humans. | 1982 | 6814070 | |
| fatal diphtheria--wisconsin. | 1982 | 6817057 | |
| [isolation and identification of corynebacterium, listeria and erysipelothrix]. | 1982 | 6817545 | |
| fatty acid composition of some mycolic acid-containing coryneform bacteria. | the fatty acid profiles of 74 strains of mycolic acid-containing coryneform bacteria were examined by gas-liquid chromatography. all of the strains contained major amounts of straight-chain and monounsaturated fatty acids although some also possessed substantial amounts of 10-methyloctadecanoic acid. iso- and anteiso-branched acids were not present. five distinct fatty acid patterns were evident: (i) corynebacterium diphtheriae, c. pseudotuberculosis and 'c. ulcerans' strains contained major amo ... | 1982 | 6818327 |
| biotypes, toxigenicity and antibiotic sensitivity of corynebacterium diphtheriae. | 1982 | 6819337 | |
| isolation of c. diphtheriae gravis in leeds. | 1982 | 6821100 | |
| diphtheria of the eye. a forgotten diagnosis. | 1982 | 6821237 | |
| [survey on corynebacterium diphtheriae carriers and susceptibility to schick's toxin in diphtheria contacts]. | 1982 | 6821284 | |
| [demonstration of a method of passive microhemagglutination for the titration of antidiphtheria antibodies]. | 1980 | 6821476 | |
| [purulent otitis media caused by corynebacterium diphtheriae]. | 1983 | 6887703 | |
| infective endocarditis due to corynebacterium diphtheriae: report of a case and review of the literature. | 1981 | 6893847 | |
| cotranslational secretion of diphtheria toxin and alkaline phosphatase in vitro: involvement of membrane protein(s). | crude messenger ribonucleic acid fractions isolated from corynebacterium diphtheriae and escherichia coli were translated in an e. coli in vitro protein-synthesizing system and yielded precursors of the secreted proteins diphtheria toxin and alkaline phosphatase, respectively. addition of inverted e. coli inner membrane vesicles to the system during the initial stages of translation resulted in the intravesicular segregation of mature diphtheria toxin and alkaline phosphatase. outer membrane ves ... | 1980 | 6988404 |
| corynebacterium diphtheriae endocarditis in an adult with congenital cyanotic heart disease. a case report. | corynebacterium diphtheriae is a very rare cause of both septicaemia and endocarditis. a case of endocarditis due to c. diphtheriae is reported and the clinical features and course of this case and other similar cases previously reported in the literature are compared. | 1982 | 7071685 |
| analysis of mycolic acids from a group of corynebacteria by capillary gas chromatography and mass spectrometry. | the cell wall of leprosy-derived corynebacteria (a group of 'diphtheroids' isolated from human leprosy lesions and patients' blood) was previously shown to contain, in addition to peptidoglycan and arabinogalactan, mycolic acids. these alpha-branched beta-hydroxy fatty acids were attributed to the corynomycolic group, according to their rf in monodimensional thin-layer chromatography. in the present work, mycolic acids from leprosy-derived and reference corynebacteria have been fractionated by m ... | 1982 | 7106128 |
| corynebacterium diphtheriae septicemia. | this report describes the case of a patient with chronic lymphocytic leukemia in whom septicemia caused by a nontoxigenic strain of corynebacterium diphtheriae developed. numerous abscesses were present in sections of liver and spleen taken at autopsy. the case is discussed, and the literature on infections caused by organisms of the genus corynebacterium is briefly reviewed. | 1981 | 7223724 |
| diphtheria in jordan: a diminishing yet important paediatric disease. | twenty-seven cases of diphtheria (15 male, 12 female) admitted over a 2-year period, 1977-1978, at the jordan university hospital were reviewed. the majority were admitted during autumn and were in the 2-9-year age group. eleven patients were not vaccinated and six were questionably vaccinated; of these seven died. in 10 patients who were partially or completely vaccinated, only one died. antitoxin was given to 23 patients at varying intervals after the onset of the disease. no antitoxin was giv ... | 1980 | 7381985 |
| [tonsils in diphtheria and the corynebacterium diphtheriae carrier state]. | investigations of 16 archive autopsy observations of diphtheria of the fauces confirmed bacteriologically revealed that corynebacteria diphtheriae multiplied mainly on the surface of the mucous membrane. fibrinous inflammation on the tonsillar surface is most typical for this agent. in all cases with manifestations of acute inflammation the lesion was caused by c. diphtheriae in combination with other bacteria, most frequently streptococci. the latter were harboured deep in the tonsills and caus ... | 1980 | 7406725 |
| diphtheria: changing patterns in the developing world and the industrialized world. | in the past, diphtheria was considered one of the most serious childhood diseases because it took a heavy toll in health and life among preschool-aged children. prior to the widespread availability of diphtheria toxoid, nearly 70% of cases were in children younger than 15 years of age. in the industrialized countries, immunization against diphtheria became widespread in the 1940s and 1950s. this led to a marked decrease in the incidence of diphtheria. there was also a decrease in circulating tox ... | 1995 | 7489768 |
| genomic organization of the mycobacterial sigma gene cluster. | we have previously described sigma a and sigma b and their structural genes, mysa and mysb, respectively, in mycobacterium smegmatis. we have now sequenced the corresponding regions in the m. tuberculosis and m. leprae chromosomes, and have found the two homologous genes. the chromosomal linkage and the deduced amino acid (aa) sequences of the two genes show very high similarity in the three species of mycobacteria. we also report the finding of two other open reading frames (orf) in these clust ... | 1995 | 7489918 |
| cutaneous diphtheria in bristol. | 1994 | 7520805 | |
| a randomized controlled trial of two acellular pertussis-diphtheria-tetanus vaccines in primary immunization in ghana: antibody responses and adverse reactions. | two acellular pertussis vaccines combined with diphtheria and tetanus toxoids (apdt vaccines) were compared with a whole cell pdt (wcpdt) vaccine in primary immunization in ghana. one is a liquid vaccine which is used for general immunization in japan and the other is a freeze-dried vaccine newly developed as a heat-stable vaccine. eighty-nine infants were recruited in the study. sixty-eight who completed three doses of the immunization were assessed for immunological responses. twenty-one dropp ... | 1994 | 7521636 |
| in vitro comparison of the effectiveness of three surface disinfectants. | choosing a general purpose surface disinfectant for the dental office environment is difficult because of the wide range of products available, varying claims by manufacturers and contradictory reports in the literature regarding product efficacy. this study tested the antimicrobial effectiveness of diluted-for-use o-phenyl-phenol (omni), didecyl dimethyl ammonium (basic g) and isopropanol (virahol). products were tested for antimicrobial activity at 0, 12 and 24 hours, 3, 6 and 8 days. o-phenyl ... | 1994 | 7523623 |
| expanded programme on immunization. diphtheria immunity in the adult french population. | 1995 | 7547210 | |
| [is extensive diphtheria vaccination necessary?]. | 1994 | 7555743 | |
| treatment of infection caused by toxigenic and non-toxigenic strains of corynebacterium diphtheriae. | 1995 | 7559184 | |
| the return of corynebacterium diphtheriae: the rise of non-toxigenic strains. | with the decline in incidence of diphtheria in europe and the usa, many laboratories no longer routinely culture throat swabs for corynebacterium diphtheriae. however, there is an outbreak of infection with toxigenic strains in russia and most adults do not have protective levels of antibody. non-toxigenic strains are known to cause local disease and lysogenic conversion probably occurs in vivo as well as in vitro. non-toxigenic c. diphtheriae var. gravis, formerly quite rare, has been isolated ... | 1995 | 7560966 |
| [endocarditis caused by corynebacterium diphtheriae tolerant to amoxicillin]. | 1995 | 7567828 | |
| structures of the apo- and the metal ion-activated forms of the diphtheria tox repressor from corynebacterium diphtheriae. | the diphtheria tox repressor (dtxr) of corynebacterium diphtheriae plays a critical role in the regulation of diphtheria toxin expression and the control of other iron-sensitive genes. the crystal structures of apo-dtxr and of the metal ion-activated form of the repressor have been solved and used to identify motifs involved in dna and metal ion binding. residues involved in binding of the activated repressor to the diphtheria tox operator, glutamine 43, arginine 47, and arginine 50, were locate ... | 1995 | 7568230 |
| the erythromycin resistance gene of the corynebacterium xerosis r-plasmid ptp10 also carrying chloramphenicol, kanamycin, and tetracycline resistances is capable of transposition in corynebacterium glutamicum. | the clinical isolate corynebacterium xerosis m82b carries the 50-kb r-plasmid ptp10 that confers resistance to the antibiotics chloramphenicol, kanamycin, erythromycin, and tetracycline. a detailed restriction map of ptp10 was constructed by cloning and analyzing restriction fragments of ptp10 in escherichia coli. the resistance determinants of ptp10 were located by studying the phenotype of the recombinant plasmids in e. coli and corynebacterium glutamicum. restriction patterns of fragments enc ... | 1995 | 7568464 |
| a simple "universal" dna extraction procedure using sds and proteinase k is compatible with direct pcr amplification. | 1995 | 7580932 | |
| diphtheria disaster relief. | 1995 | 7585198 | |
| [seroepidemiological surveillance and methodological study of pertussis, diphtheria and tetanus]. | in this paper, we studied the diagnostic method of dpt using pa method developed by japanese national institute of health (nih) in 1993-1994, meanwhile we surveyed the antibody level of dpt. we collected 900 sera samples and 300 sera samples from hebei province in 1993 and 1994. the result showed that pa method was simple, reliable and easy to observe, which can make up the shortages existed in pha method. the antitoxin titers obtained by the pa method correlated well with the pha method, while ... | 1995 | 7585903 |
| characterization of an iron-dependent regulatory protein (ider) of mycobacterium tuberculosis as a functional homolog of the diphtheria toxin repressor (dtxr) from corynebacterium diphtheriae. | the dtxr protein from corynebacterium diphtheriae is an iron-dependent repressor that regulates transcription from the tox, irp1, and irp2 promoters. a gene from virulent mycobacterium tuberculosis h37rv was recently shown to encode a protein, here designated iron-dependent regulator (ider), that is almost 60% homologous to dtxr from c. diphtheriae. a 750-bp pcr-derived dna fragment carrying the m. tuberculosis ider allele was subcloned to both high- and low-copy-number vectors. in escherichia c ... | 1995 | 7591059 |
| polymerase chain reaction for the detection of toxigenic corynebacterium diphtheriae. | several conventional methods have been described for the detection of corynebacterium diphtheriae toxin, including elek immunodiffusion, tissue culture using vero cells and guinea pig inoculation. all these methods have the disadvantage of being either slow to complete or technically demanding, particularly when performed infrequently. we examined 64 strains of c. diphtheriae by pcr and elek immunodiffusion, and strains showing a positive result in either assay were inoculated into guinea pigs. ... | 1995 | 7603758 |
| molecular epidemiology of corynebacterium diphtheriae from northwestern russia and surrounding countries studied by using ribotyping and pulsed-field gel electrophoresis. | a selection of 100 corynebacterium diphtheriae isolates from asymptomatic carriers and clinical cases from five regions in northwestern russia were examined. six additional isolates from patients in finland and estonia with epidemiological links to russia were also examined. all isolates were characterized by biotyping, toxigenicity testing, ribotyping, and pulsed-field gel electrophoresis (pfge). hybridization of genomic dna digested with bsteii revealed five ribotype patterns among the biotype ... | 1995 | 7615709 |
| [immunity to diphtheria in young adults in spain]. | 1995 | 7623498 | |
| transition metal ion activation of dna binding by the diphtheria tox repressor requires the formation of stable homodimers. | the diphtheria tox repressor (dtxr) is a transition metal ion-dependent regulatory element that controls the expression of diphtheria toxin and several genes involved in the synthesis of siderophores in corynebacterium diphtheriae. in the presence of transition metal ions apo-dtxr becomes activated and specifically binds to its target dna sequences. we demonstrate by glutaraldehyde cross-linking that monomeric apo-dtxr is in weak equilibrium with a dimeric form and that upon addition of activati ... | 1995 | 7624323 |
| corynebacterium diphtheriae osteomyelitis in an immunocompetent child: a case report. | septic osteomyelitis of the hip in a previously healthy child is described. a weakly toxigenic corynebacterium diphtheriae strain was isolated from the bone aspirate. the results of the treatment were rapidly satisfactory, after surgical drainage and antibiotic therapy with pristinamycin. conclusion: this case report shows that c. diphtheriae has not disappeared in the developed world and can be responsible of systemic infections. | 1995 | 7641771 |
| [corynebacterium diphtheriae endocarditis complicated by septic arthritis and cerebral abscess]. | the authors report a rare case of the mitis type corynebacterium diphteriae endocarditis on a prosthetic valve complicated by septic arthritis and cerebral abscess. the authors underline the importance of regular transoesophageal echocardiographic control and underline the diagnostic value of ultrafast computed tomography for the diagnosis of aortic annular and interventricular septal abscesses in patients with mechanical prosthetic valves. | 1995 | 7646303 |
| cutaneous diphtheria in a homeless man. | 1995 | 7651047 | |
| farnesylation of caax-tagged diphtheria toxin a-fragment as a measure of transfer to the cytosol. | diphtheria toxin binds to receptor-positive cells through its b-fragment, the toxin is then endocytosed, and the low ph in endosomes triggers the translocation of the enzymatically active a-fragment to the cytosol. a synchronous release of a-fragments into the cytosol can be induced by exposing cells with surface-bound toxin to low ph. we have used this protein translocation system to develop a novel method to study whether or not a protein is exposed to the cytosol. protein farnesylation is a c ... | 1995 | 7669773 |
| cerebral infarction in a case of diphtheria. | 1995 | 7673755 | |
| [diphtheria in poland. epidemiologic situation of diphtheria in poland before 1992]. | 1995 | 7676054 | |
| a diphtheria epidemic in lesotho, 1989. did vaccination increase the population's susceptibility? | we report a diphtheria outbreak which occurred in the 1st 6 months of 1989 in the quthing district of lesotho in southern africa. sixty-eight clinical cases were identified in patients with ages ranging from 14 months to 51 years. the 10-15-year age group represented 38% of the cases and the 15-20-year age group another 26%. age-adjusted incidence rates were higher in patients aged between 15 and 35 than in those aged 0-15 years (p < 0.001). the overall case fatality rate was 23%. most deaths oc ... | 1993 | 7681640 |
| diphtheria in the united kingdom--two recent incidents. | 1993 | 7691357 | |
| [the effect of toxic substances from diphtheria corynebacteria on human platelet aggregation]. | 1994 | 7703459 | |
| are we ready for diphtheria? a report from the diphtheria diagnostic workshop, atlanta, 11 and 12 july 1994. | 1995 | 7706801 | |
| effect of malnutrition in ecuadorian children on titers of serum antibodies to various microbial antigens. | the titers of serum antibodies to natural infection with enteric and respiratory pathogens, to a food antigen and to tetanus and diphtheria toxoid were evaluated by enzyme-linked immunosorbent assay in 1,554 ecuadorian children younger than 5 years of age. the nutritional status of the children was assessed by anthropometry and measurement of biochemical status indicators. the children were enrolled in a representative national nutrition and health survey. antibody titers were analyzed as a func ... | 1995 | 7719915 |
| taxonomy of corynebacterium diphtheriae and related taxa, with recognition of corynebacterium ulcerans sp. nov. nom. rev. | levels of genomic dna relatedness were determined using a s1 nuclease procedure for reference bacteria identified as biotypes of corynebacterium diphtheriae, biovars of corynebacterium pseudotuberculosis, and 'corynebacterium ulcerans'. these results showed that the three species are separate taxa at the genomospecies level whereas biotypes and biovars are closely related genomically within each species. phylogenetic analyses of small-subunit rdna sequences revealed that 'corynebacterium ulceran ... | 1995 | 7729671 |
| the in-vitro susceptibilities of toxigenic strains of corynebacterium diphtheriae isolated in northwestern russia and surrounding areas to ten antibiotics. | the in-vitro activities of ten antibiotics against 83 toxigenic strains of corynebacterium diphtheriae recently isolated in northwestern russia and surrounding areas were determined by an agar dilution method. all of the strains were susceptible to erythromycin, penicillin, ampicillin, cefuroxime, chloramphenicol, ciprofloxacin, gentamicin and tetracycline. trimethoprim and rifampicin were each active against 81 isolates, the two strains resistant to the latter agent having been isolated from tw ... | 1994 | 7730218 |
| [use of a dna-dna hybridization method for studying corynebacterium diphtheriae strains]. | in addition to traditional tests, a battery of dna probes was used to characterize 134 toxigenic and 125 nontoxigenic c.diphtheriae strains isolated in russia in 1986-1989. 2.5% of nontoxigenic strains carried the determinants of both toxin subunits a and b, and 20% possessed at least a fragment of the gene determining toxin synthesis. optimal conditions of hybridization technology were found. the method may be used as an alternative to traditional techniques in diagnostic studies and screenings ... | 1994 | 7739593 |
| three-dimensional structure of the diphtheria toxin repressor in complex with divalent cation co-repressors. | when corynebacterium diphtheriae encounters an environment with a low concentration of iron ions, it initiates the synthesis of several virulence factors, including diphtheria toxin. the diphtheria toxin repressor (dtxr) plays a key role in this iron-dependent, global regulatory system and is the prototype for a new family of iron-dependent repressor proteins in gram-positive bacteria. this study aimed to increase understanding of the general regulatory principles of cation binding to dtxr. | 1995 | 7743135 |
| [method of immunoenzyme analysis in the diagnosis of diphtheria]. | enzyme immunoassay (eia) was used for the diagnosis of diphtheria. the data indicate the specificity and reliability of the method and prove the necessity of its use for the diagnosis of diphtheria and with prophylactic purposes in screening of certain populations. | 1995 | 7757365 |
| high-yield production of diphtheria toxin mutants by high-density culture of c7 (beta)tox+ strains grown in a non-deferrated medium. | a high-density growth approach was utilized to produce mutated diphtheria toxin from two strains of corynebacterium diphtheria: c7 (beta)(tox-201,tox-9) and c7 (beta)(tox-107). the cross-reacting mutants (crm) of the diphtheria toxin are crm9 and crm107; both of them carry the mutation in their binding site and, as a result, have 1/300 of the systemic toxicity of the wild-type diphtheria toxin. since iron inhibits diphtheria toxin production, the traditional approach has been to grow the bacteri ... | 1995 | 7766139 |
| cloning of a corynebacterium diphtheriae iron-repressible gene that shares sequence homology with the ahpc subunit of alkyl hydroperoxide reductase of salmonella typhimurium. | to understand how corynebacterium diphtheriae responds to iron limitation, we compared the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) protein profiles of both wild-type cells and iron uptake mutants grown in either high- or low-iron medium. the removal of iron by ethylene diamine di-(o-hydroxy-phenyl acetic acid) from the growth medium of wild-type cells resulted in induction of at least 14 polypeptides. dira, a major iron-repressible polypeptide, was purified from wild ... | 1995 | 7768861 |
| [the adsorbed-dtp-m vaccination of children with allergically altered reactivity]. | immunization and booster immunization with adsorbed dpt-m vaccine was carried out, respectively, in 45 and 15 children with different severity of the course of an allergic disease, each child receiving simultaneously individually prescribed complex drug therapy. the postvaccinal period took a relatively favorable course. general and allergic reactions and complications observed after the injection of the preparation did not hinder the continuation of the course of immunization, while the appeara ... | 1995 | 7778374 |
| combination of dtp and haemophilus influenzae type b conjugate vaccines can affect laboratory evaluation of potency and immunogenicity. | a commonly used diphtheria-tetanus-pertussis (whole-cell) vaccine was combined with each of three different haemophilus influenzae type b (hib) capsular polysaccharide vaccines. each hib vaccine incorporated one of three different protein conjugates: tetanus toxoid, diphtheria crm197 toxoid or group b neisseria meningitidis outer membrane vesicles. the effects of these combinations on the subsequent laboratory control testing were examined. the addition of the hib vaccines had no significant eff ... | 1994 | 7779360 |
| definition of minimum standards for immunogenicity--potential problems of serological correlates--possible interference. serological correlates for diphtheria-tetanus-whole cell pertussis vaccines. | 1994 | 7779366 | |
| molecular cloning, dna sequence analysis, and characterization of the corynebacterium diphtheriae dtxr homolog from brevibacterium lactofermentum. | a homolog of the corynebacterium diphtheriae dtxr gene was isolated from brevibacterium lactofermentum. the product of the b. lactofermentum dtxr gene was immunoreactive with polyclonal anti-dtxr antibodies and functioned as an iron-activated repressor capable of regulating the expression of beta-galactosidase from a diphtheria tox promoter/operator transcriptional fusion in recombinant escherichia coli. the extents of induction by increasing concentrations of the chelator 2,2'-dipyridyl were id ... | 1995 | 7814338 |
| iron, dtxr, and the regulation of diphtheria toxin expression. | in recent years considerable advances have been made in the understanding of the molecular basis of iron-mediated regulation of diphtheria toxin expression. the tox gene has been shown to be regulated by the heavy metal ion-activated regulatory element dtxr. in the presence of divalent heavy metal ions, dtxr becomes activated and binds to a 9 bp interrupted palindromic sequence. the consensus-binding site has been determined by both the sequence analysis of dtxr-responsive operators cloned from ... | 1994 | 7830565 |
| [macrolides. new therapeutic prospects]. | the aim of the development of semisynthetic derivatives was to overcome the problem of chemical stability of erythromycin a in acid medium, with less variability in gastro-intestinal absorption and leading to renewed interest in macrolides. the new macrolides have the same antibacterial spectrum as erythromycin a including gram-positive and gram-negative cocci, intracellular bacteria, mycoplasma, campylobacter sp., helicobacter pylori, mycobacteria spp., gram-negative bacilli including haemophil ... | 1994 | 7831266 |
| effect of neonatal immunization with diphtheria and tetanus toxoids on antibody responses to haemophilus influenzae type b conjugate vaccines. | we randomly assigned 150 newborn infants to receive diphtheria and tetanus toxoids (dt) or hib oligosaccharide conjugate (hboc) at birth to determine whether exposure to the haemophilus influenzae type b (hib) conjugate vaccines' carrier proteins would enhance immune responses to subsequent administrations of hboc or prp-tetanus toxoid conjugate (prp-t) at 2, 4, and 6 months of age. their antibody responses were compared with those of 100 children immunized with hboc or prp-t beginning at 2 mont ... | 1995 | 7844665 |
| the superoxide dismutase gene, a target for detection and identification of mycobacteria by pcr. | the superoxide dismutase gene has been identified as a target in screening for the presence of mycobacteria on the genus level and differentiating relevant mycobacterial species from one another by pcr. consensus primers deduced from known superoxide dismutase gene sequences allowed the amplification of dnas from a variety of bacteria, fungi, and protozoa. selected amplicons from actinomyces viscosus, corynebacterium diphtheriae, corynebacterium pseudodiphtheriticum, mycobacterium avium, m. fort ... | 1994 | 7852575 |
| [corynebacterium diphtheriae endocarditis complicated by arthritis and cerebral abscess]. | 1994 | 7864526 | |
| interchangeability of conjugated haemophilus influenzae type b vaccines in infants. | to evaluate the safety and immunogenicity of two haemophilus influenzae type b (hib) conjugate vaccines when administered in serial combination. these vaccines consisted of hib capsular polysaccharide polyribosyl-ribitol phosphate (prp) conjugated to the meningococcal outer membrane protein (omp) complex (prp-omp) and h influenzae oligosaccharide conjugated to a mutant toxin (crm197) isolated from corynebacterium diphtheriae (hboc). | 1995 | 7869554 |
| [the characteristics of the immune response in children having different hla phenotypes after the first revaccination with adtp]. | 1994 | 7879562 | |
| [non-toxic corynebacterium diphtheriae septicemia with endocarditis in an earlier healthy adult. first case and review of the literature]. | corynebacterium diphtheriae septicaemia is rarely encountered, usually in very particular situations: children with severe congenital heart disease or after heart surgery. rare cases have been reported in immunodepressed adults or drug addicts. we observed a case in a formerly healthy 41-year-old woman who was hospitalized for fever unresponsive to bacampicillin. in this patient, no portal of entry could be identified; there was no history of past surgery nor drug abuse. the patient was not immu ... | 1994 | 7899317 |
| [a familial case of diphtheria of the skin]. | 1994 | 7900013 | |
| molecular evidence of person-to-person transmission of a pigmented strain of corynebacterium striatum in intensive care units. | during a 14-month period, a unique strain of corynebacterium striatum that produces a diffusible brown pigment was isolated from purulent sputa of nine patients and from nonrespiratory sites of two additional patients. seven nonpigmented clinical isolates from the same period and three reference strains of c. striatum were compared with the brown isolates. most patients had multiple sputum cultures with no coryneforms before the brown strain emerged, suggesting that the organism was hospital acq ... | 1994 | 7907342 |
| [detection by molecular biology techniques of diphtheria toxin production in corynebacteria]. | the ability to secrete a potent protein-toxin called diphtheria toxin (dt) is of outstanding importance for the outcome of diphtheria epidemics. rapid molecular-biological screening methods are shown that can improve the differentiation between toxinogenic and non-toxinogenic corynebacterium diphtheriae strains. the recently reported infection with an endemic, putative toxinogenic corynebacterium diphtheriae strain in lower saxony is discussed. | 1994 | 7919700 |
| determination of the minimal essential nucleotide sequence for diphtheria tox repressor binding by in vitro affinity selection. | the expression of diphtheria toxin in lysogenic toxigenic strains of corynebacterium diphtheriae is controlled by the heavy metal ion-activated regulatory protein dtxr. in the presence of divalent heavy metal ions, dtxr specifically binds to the diphtheria tox operator and protects a 27-bp interrupted palindromic sequence from dnase i digestion. to determine the consensus dna sequence for dtxr binding, we have used gel electrophoresis mobility-shift assay and polymerase chain reaction (pcr) ampl ... | 1994 | 7937822 |